Digital Commons Network^™

Early Adolescent Nicotine Exposure Has Long-Lasting Effects On Cocaine-Induced Behaviors In Mice, Mai Alajaji

Theses and Dissertations

Nicotine is one of the most commonly used drugs among adolescent populations. Given the fact that adolescence is a unique developmental stage, during which nicotine has long-term effects on future drug-taking behavior, it is essential to understand how early exposure to nicotine during adolescence may affect the abuse liability of other drugs. We hypothesize that repeated exposure to low doses of nicotine in adolescence induce age-specific enhancement of the rewarding effects of several drugs of abuse in the conditioned place preference (CPP) test. Furthermore, we predict that these changes in behavioral responses are mediated by nicotine-induced brain region-specific increases in …

Kinetics And Mechanisms Of Accumulation For Liposomal Ciprofloxacin Into Rat Alveolar Macrophages, Sayeed Mossadeq

Theses and Dissertations

The kinetics and mechanism of accumulation for liposomal ciprofloxacin (Lipo-CPFX) into the rat alveolar macrophage NR8383 cells were studied in vitro, in comparison to unformulated ciprofloxacin (CPFX). Upon incubation with CPFX or Lipo-CPFX, cellular drug accumulation was determined from the cell lysates or efflux was from the extracellular media by fluorescence-HPLC. The accumulation for Lipo-CPFX reached the asymptotic values at ≥ 2 hours, which was a result of uptake and efflux. The uptake appeared to be due to liposomes, mediated via cellular energy-independent mechanism like lipid fusion. In contrast, the efflux appeared to be due to ciprofloxacin, partly cellular energy-dependent, …

The Impact Of Emotional Intelligence On Auditor Judgment, Yang Ling

Theses and Dissertations

Emotions are an important underlying factor that may interact with pressure and other situational variables to influence auditors’ judgments and decisions. This study seeks to identify emotional intelligence (EI) as a key factor in dealing with emotions and pressures in an audit context. In this paper, I focus on how EI may influence the relation between job pressures (i.e., time budget pressure and client incentive pressure) on the auditor’s judgment. Specifically, I investigate the moderating effect of emotional intelligence on auditor judgments when auditors experience both internal and external pressures. The results suggest that the moderating influence of EI on …

Elucidation Of Mechanisms Generating 5-Hydroxymethylcytosine (5hmc) In Mammalian Mitochondria, Prashant Thakkar

Theses and Dissertations

DNA methylation plays a pivotal role in governing cellular processes including genomic imprinting, gene expression, and development. Recently, the Tet family of methylcytosine dioxygenases(Tet1, Tet2 and Tet3) was found to catalyze the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), an intermediate in the pathway of DNA demethylation. Tet enzymes catalyze this hydroxylation in a 2-oxoglutarate and Fe2+ dependent manner. We have recently reported significant levels of 5mC and 5hmC modification in immunoprecipitates of mammalian mitochondrial DNA(mtDNA). We provide the first evidence that a DNA Methyltransferase-1 isoform (mtDNMT1) translocates to the mitochondria using an N-terminal mitochondrial targeting sequence. mtDNMT1 expression is …

Calcium/Calmodulin-Dependent Protein Kinase Ii Beta (Camkiiβ): A Regulator Of Oligodendrocyte Maturation And Myelination, Christopher Waggener

Theses and Dissertations

Oligodendrocytes are cells located in the central nervous system (CNS) that are responsible for the production of the lipid rich membrane, myelin. Myelin and the process of making and wrapping myelin around an axon (also known as myelination) are critical for normal development since they ensure proper signal conduction in the vertebrate CNS. The loss or damage of this myelin, which is typically associated with the demyelinating disease multiple sclerosis (MS), is associated with improper axonal protection along with disrupted nerve signaling which can lead to a variety of different debilitating phenotypic responses. It has been shown that there are …

The Role Of The Nucleosome Remodeling And Histone Deacetylase (Nurd) Complex In Fetal Γ-Globin Expression, Maria Amaya

Theses and Dissertations

An understanding of the human fetal to adult hemoglobin switch offers the potential to ameliorate β-type globin gene disorders such as sickle cell anemia and β-thalassemia through activation of the fetal γ-globin gene. Chromatin modifying complexes, including MBD2-NuRD and GATA-1/FOG-1/NuRD play a role in γ-globin gene silencing, and Mi2β (CHD4) is a critical component of NuRD complexes. In the studies presented in Chapter 2, we observed that the absence of MBD2 in a sickle cell mouse model leads to a decrease in the number of sickled cells observed in the peripheral blood, and significantly increases survival in these mice. Although …

Hormone Epimers Regulate Er Stress And Core Regulatory Genes: Network Analysis With Applications To Glioma And Chronic Pressure Ulcers, Thomas L. Shaak

Theses and Dissertations

DHEA has been determined to have medically significant activity and is the parent compound to the more active metabolites; 17α-AED, 17β-AED and 17β-AET, which exhibit strong biological activity that has been attributed to androgenic, estrogenic or anti-glucocorticoid activity in vivo and in vitro. This study compared DHEA, 17α-AED, 17β-AED and 17β-AET for their ability to activate the human AR, ER and GR receptors and determine the relative androgenicity, estrogenicity and glucocorticoid activity. The results show that, at the receptor level, these androstene hormones are weak AR and even weaker ER activators. Direct androstene hormone activation of the human AR, ERα, …

Automated Detection Of Incomplete Exhalation As An Indirect Detection Of Auto-Peep On Mechanically Ventilated Adults, Nyimas Arief

Theses and Dissertations

Auto-PEEP is auto positive end-expiratory pressure due to excessive amounts of alveolar gas produced by sustained recurrent incomplete exhalation. Incomplete exhalation occurs when the exhaled breath never reaches a flow rate of 0 L/min. The objective of this dissertation is to develop an automated detection system of auto-PEEP through incomplete exhalation as revealed by ventilator graphics for mechanically ventilated adults. Auto-PEEP can cause adverse effects if allowed to linger and if not quickly identified. An automated detection system will be instrumental in helping to quickly identify auto-PEEP. A computerized algorithm was developed to detect incomplete exhalation based on the following …

In-Vitro Pk/Pd Profiling And Modeling Of The Anti-Sickling Agents, 5-Hydroxymethyl Furfural (5-Hmf) And Novel Synthetic Allosteric Effectors Of Hemoglobin (Aeh) In Human Whole Blood, Apurvasena Parikh

Theses and Dissertations

Introduction. 5-HMF and novel INN-compounds are left-shifting AEH, shown to have anti-sickling action by forming transiently covalent Schiff-base adducts with hemoglobin (Hb), thereby increasing the Hb O2-affinity. They are hypothesized to be substrates for aldehyde dehydrogenase (ALDH) in the liver and red blood cells (RBC). Methods. Biopharmaceutical assessments were made for AEH, using calculated physicochemical properties. Their in-vitro hepatic metabolism (mediated by ALDH) was characterized using hepatic cytosol, and in-vitro-in-vivo extrapolations (IVIVE) were made. Inter-species differences in hepatic cytosolic ALDH activity were investigated using acetaldehyde as a model substrate in different mammalian species. Time- and concentration-dependent in-vitro disposition of 5-HMF …

Association Between Concomitant Use Of Bisphosphonates And Serotonin Reuptake Inhibitors And Increased Risk Of Osteoporotic-Related Fractures: Among Community-Dwelling Postmenopausal Women, Abner Nyandege

Theses and Dissertations

Osteoporosis and depression are prevalent among older postmenopausal women 65 years or older. Bisphosphonates (BPs) and selective serotonin reuptake inhibitors (SSRIs) or serotonin norepinephrine reuptake inhibitors (SNRIs) are commonly used medications to treat these conditions. Inhibitory effects of BPs on osteoclasts are responsible for the reduction in fracture risk. SSRIs, however, are associated with increased fracture risk through decreasing osteoblasts and increasing osteoclastic activity. These effects of SSRIs could attenuate the beneficial effects of BPs. This dissertation describes the concomitant use of BPs and SSRIs among postmeopausa women and reports findings from examining the association between concomitant use of BPs …

The Metabolic Transitions Regulated By The Estrogen-Related Receptor (Err) In Drosophila Melanogaster, Yan Li

Theses and Dissertations

In multicellular organism, bioenergetic metabolism is strictly regulated toward efficient generation of ATP. However, in certain situations, such as in limiting oxygen or in the rapidly proliferating system like growing juvenile or cancer cells, organisms apply the metabolic strategy that favors the production of biomass (e.g., nucleotides, amino acids, and lipids) over efficiency of ATP generation. The conserved estrogen-related receptors (ERRs) are master regulators in controlling metabolic homeostasis, and good candidates for mediating the metabolic transition induced by hypoxia and development. First, we investigate how dERR influences hypoxic adaptation in Drosophila melanogaster. We find that dERR is required for a …

Association Of Family Structure And Glycemic Control In Adolescents With Type 1 Diabetes: Risk And Protective Factors, Laura Jean Caccavale

Theses and Dissertations

Youth with type 1 diabetes (T1D) from single-parent families are more likely to be in poorer glycemic control (HbA1c). Demographic trends indicate more households are composed of unmarried adults and fewer youths. Family density, or a youth: adult ratio, may be a more salient factor than single-parent status in the association with glycemic control. Data from 257 adolescents aged 11-14 years (M = 12.84) at two different sites were collected as part of a randomized control trial of a treatment intervention designed to increase parent involvement and prevent deterioration of adolescent diabetes disease care. Single-parent status was determined by parental …

In Vitro Lung Epithelial Cell Transport And Anti-Inflammatory Activity For Liposomal Ciprofloxacin, Ruba Darweesh

Theses and Dissertations

Liposomal ciprofloxacin (Lipo-CPFX) is being developed for inhalation, with a goal of sustaining the therapeutic activity, compared to unformulated ciprofloxacin (CPFX). However, the kinetics and mechanism of its sustained local lung retention and pharmacological activity are yet to be fully characterized. This project hypothesized that Lipo-CPFX enables slower and sustained lung epithelial transport and uptake, compared to CPFX, thereby producing prolonged local pharmacological actions. The human bronchial epithelial Calu-3 cells were used as monolayers to characterize the kinetics and mechanism of transport and/or uptake, and to assess the effects of such slow kinetics for Lipo-CPFX on its inhibition against lipopolysaccharide …

Are There Carcinogens In Your Workplace? It's Time To Act!, France Labrèche, Brigitte Roberge, Sabrina Gravel, Marie-France D'Amours

Guides

No abstract provided.

Organizational Practices Leading To A Positive Safety Culture: A Delphi Approach, Andrew Matthew Cwalina

HCBE Theses and Dissertations

A positive safety culture has been shown to contribute to a firm's ability to avoid or reduce the occurrence of occupational accidents and injuries. In American workplaces alone 3,582 people died and 5.1 million people were disabled in 2009 and the cost to corporate America was $169 billion and an additional productivity loss of 95 million work days. The economic cost to each American household is about $1,200. Firms that establish and maintain a positive safety culture are able to achieve a competitive advantage in the market.

While much research exists showing the relationship between safety culture and accident reduction, …

Internal Brand Training In The Learning Industry, Audrey Ellison

HCBE Theses and Dissertations

Companies in the learning industry are in the business-to-business marketplace and the companies focus on providing services to their customers. In service businesses delivering on the brand promise is aligned with the services employees deliver to customers. The learning industry was used to research whether a commitment to internal brand training impacts business performance. Two of the initial hypotheses were supported; a learning orientation increases commitment to internal brand training and internal brand training increases employee commitment to the brand. The third hypothesis, employee brand commitment improves business performance, was not supported. A re-specified model found.

* Learning orientation directly …

Time Resolved Gain And Excess Noise Properties Of Ingaas/Inalas Avalanche Photodiodes With Cascaded Discrete Gain Layer Multiplication Regions, Georges M. Williams, David A. Ramirez, Majeed M. Hayat, Andrew S. Huntington

Electrical and Computer Engineering Faculty Research and Publications

To predict pulse detection performance when implemented in high speed photoreceivers, temporally resolved measurements of a 10-stage InAlAs/InGaAs single carrier multiplication (SCM) avalanche photodiode (APD)'s avalanche response to short multi-photon laser pulses were explained using instantaneous (time resolved) pulse height statistics of the device's impulse response. Numeric models of the junction carrier populations as a function of the time following injection of a primary photo-electron were used to create the probability density functions (pdfs) of the instances of the avalanche buildup process. The numeric pdfs were used to generate low frequency gain and excess noise models, which were in good …

Distinguished Brief: People Of The State Of Michigan V. David Mark Cole, J.J. Prescott, Mirian J. Aukerman, Michael J. Steinberg, Kary Moss, John Minock

Articles

This amicus brief on behalf of the Criminal Defense Lawyers of Michigan and the American Civil Liberties Union (ACLU) of Michigan argues that defendants accused of first- or second-degree criminal sexual conduct in Michigan must be made aware that pleading guilty will result in mandatory, lifetime electronic monitoring after their release in order for any plea to be valid under Michigan law and the U.S. Constitution. To be lawful, guilty pleas must be knowing and voluntary, which requires that defendants be informed of any punishment that would be a direct consequence of their guilty plea. Lifetime electronic monitoring is a …

Boilerplate, Freedom Of Contract, And "Democratic Degradation", Brian H. Bix

Articles

In Margaret Jane Radin's provocative new book, Boilerplate: The Fine Print, Vanishing Rights, and the Rule of Law, 1 the author offers scathing observations regarding the motivation and effects of the terms placed in consumer and employee form contracts and on-line agreements. She argues that the current contracting practices make a mockery of consent, and undermine the rule of law. 2 Boilerplate's essential claim is that for many contracting parties, freedom of contract is less an ideal than a sham. 3 The book properly criticizes theories of contract law (and courses in contract law) that largely ignore boilerplate and its …

Costly Mistakes: Undertaxed Business Owners And Overtaxed Workers, Mary Louise Fellows, Lily Khang

Articles

This Article advocates fundamental changes in the federal income tax base by systematically challenging conventional understandings of consumption and investment. As signaled by its title, “Costly Mistakes,” this Article's thesis has to do with the disparate treatment of expenditures incurred by business owners and workers. Where the current tax law treats a business owner's expenditure as investment, the Article sometimes finds consumption and questions why the law should allow the expenditure to be deducted. Where the tax law treats a worker's expenditure as consumption, the Article sometimes finds investment and questions why the law does not allow at least a …

Erie's Starting Points: The Potential Role Of Default Rules In Structuring Choice Of Law Analysis, Allan Erbsen

Articles

This contribution to a symposium marking the seventy-fifth anniversary of Erie Railroad Company v. Tompkins is part of a larger project in which I seek to demystify a decision that has enchanted, entangled, and enervated commentators for decades. In prior work I contended that the “Erie doctrine” is a misleading label encompassing four distinct inquiries that address the creation, interpretation, and prioritization of federal law and the adoption of state law when federal law is inapplicable. This article builds from that premise to argue that courts pursuing Erie’s four inquiries would benefit from default rules that establish initial assumptions and …

Strategic And Educational Leadership Of Al-Ain School Principals In Accordance With The New School Model., Aysha Saeed Mohammed Al Nuaimi

Theses

This study aims at identifying the roles of school principal according the new school model applied by Abu Dhabi Educational Council, and the extent of applying these roles in the field; more to the point, identifying any gaps between what is requested from the principals and what is really presented. The aforementioned aims are to be reached through answering the following questions:

1. To what extent do principals in Al Ain apply the strategic and educational leadership standards according to the new school model, from principals’ perspective?
2. To what extent do principals in Al Ain apply the strategic and educational leadership …

Investigating English Teachers’ Views, Actual Practice And The Connection Between Them Regarding The Use Of Arabic During English Classes In The Uae High Schools, Ameirah Hassan Al Mohamed

Theses

This study investigated English Teachers’ views, actual practices and the connections among them regarding the use of Arabic during teaching English as foreign language in one of the Northern Emirates in the UAE high schools. The present study which took place in one Emiratis in UAE adopted Activity Theory (AT) to investigate English teachers’ views and their real practices on using Arabic while teaching English. The study employed a mixed method research design involving a survey, semi-structured interview and classroom observation. The study focused on the following research questions: 1) What are the English teachers’ views on using Arabic during …

Intraguild Relationships Between Sympatric Predators Exposed To Lethal Control: Predator Manipulation Experiments, Benjamin L. Allen, Lee R. Allen, Richard M. Engeman, Luke K.-P. Leung

United States Department of Agriculture Wildlife Services: Staff Publications

Introduction: Terrestrial top-predators are expected to regulate and stabilise food webs through their consumptive and non-consumptive effects on sympatric mesopredators and prey. The lethal control of top-predators has therefore been predicted to inhibit top-predator function, generate the release of mesopredators and indirectly harm native fauna through trophic cascade effects. Understanding the outcomes of lethal control on interactions within terrestrial predator guilds is important for zoologists, conservation biologists and wildlife managers. However, few studies have the capacity to test these predictions experimentally, and no such studies have previously been conducted on the eclectic suite of native and exotic, mammalian and reptilian …

Immunological And Clinical Response Of Coyotes (Canis Latrans) To Experimental Inoculation With Yersinia Pestis, Laurie A. Baeten, Ryan Pappert, John Young, Martin E. Schriefer, Thomas Gidlewski, Dennis Kohler, Richard A. Bowen

United States Department of Agriculture Wildlife Services: Staff Publications

Multiple publications have reported the use of coyotes (Canis latrans) in animalbased surveillance efforts for the detection of Yersinia pestis. Coyotes are likely exposed via flea bite or oral routes and are presumed to be resistant to the development of clinical disease. These historic data have only been useful for the evaluation of the geographic distribution of Y. pestis in the landscape. Because the canid immunologic response to Y. pestis has not been thoroughly characterized, we conducted experimental inoculation of captive-reared, juvenile coyotes (n58) with Y. pestis CO92 via oral or intradermal routes. We measured the humoral response …

Spatial Ecology Of Urban Raccoons In Northeastern Ohio: Implications For Oral Rabies Vaccination, Are R. Berentsen, Mike R. Dunbar, Chadd E. Fitzpatrick, W. David Walter

United States Department of Agriculture Wildlife Services: Staff Publications

In 1977, rabies was detected in a raccoon (Procyon lotor) in West Virginia, and since the mid-1980s raccoon variant rabies has spread throughout the eastern United States and moved west as far as the eastern edge of Cleveland, Ohio. The primary tool to combat this spread is the distribution of oral rabies vaccine (ORV) baits. A thorough knowledge of raccoon space use is critical in determining bait placement, particularly in urban areas. We monitored nine raccoons in urban areas of Cleveland, Ohio, calculated home range sizes, monitored raccoon movement with respect to potential movement barriers, and used resource …

Rabies, Canine Distemper, And Canine Parvovirus Exposure In Large Carnivore Communities From Two Zambian Ecosystems, Are R. Berentsen, Mike R. Dunbar, Matthew S. Becker, Jassiel M'Soka, Egil Droge, Nicholas M. Sakuya, Wigganson Matandiko, Rachel Mcrobb, Cathleen A. Hanlon

United States Department of Agriculture Wildlife Services: Staff Publications

Disease transmission within and among wild and domestic carnivores can have significant impacts on populations, particularly for threatened and endangered species. We used serology to evaluate potential exposure to rabies virus, canine distemper virus (CDV), and canine parvovirus (CPV) for populations of African lions (Panthera leo), African wild dogs (Lycaon pictus), and spotted hyenas (Crocuta crocuta) in Zambia’s South Luangwa National Park (SLNP) and Liuwa Plain National Park (LPNP) as well as community lands bordering these areas. In addition, domestic dogs in the study region were evaluated for exposure to CDV and rabies. We provide …

The Two-Phase Arterial Blood Flow With Or Without A Catheter And In The Presence Of A Single Or Multi Stenosis, Ani E. Garcia, Daniel N. Riahi

School of Mathematical & Statistical Sciences Faculty Publications

We consider the problem of blood flow in an artery with or without a catheter and in the presence of single or multi stenosis whose shape is based on the available experimental data for the stenosis in a human’s artery. The presence of stenosis in the artery, which locally narrows portion of the artery, can be a result of fatty materials such as cholesterol in the blood. The use of catheter is important as a standard tool for diagnosis and treatment in patience whose blood flow passage in the artery is affected adversely by the presence of the stenosis within …

Rethinking Critical Mass In The Federal Appellate Courts., Laura Moyer

Faculty and Staff Scholarship

This article draws from critical mass studies of gender in other political institutions to inform an application to the US Courts of Appeals. The results demonstrate the utility of considering court-level aspects of diversity. As mixed-sex panels become more common within a circuit, both male and female judges increasingly support plaintiffs in civil rights claims, though the magnitude of the effect is larger for women. The presence of a female chief judge is also positively associated with pro-plaintiff decisions by men and women in sex discrimination cases.

Study Of Dynamic Features Of Highly Energetic Reactions By Dsc And High-Speed Temperature Scanner (Hsts), Mkhitar Hobosyan, Kh. G. Kirakosyan, S. L. Kharatyan, Karen S. Martirosyan

Physics & Astronomy Faculty Publications

The dynamic features of Al2O3 - polytetrafluoroethylene (PTFE) and Al - PTFE reactions in non-isothermal conditions are presented. The Differential Scanning Calorimetry (DSC) and High-Speed Temperature Scanner (HSTS) were used to characterize the Al2O3/Al – PTFE reactions at different heating rates. The study shows that the HSTS instrument can give more information about the reaction mechanism and kinetics than the conventional DSC measurements. In this work we show that high heating rates may reveal exothermic reaction between Al2O3 and PTFE that were previously unidentified. The PTFE can potentially remove the oxide layer from aluminum in the initial period of the …