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Secondary Traumatic Stress In Baccalaureate Nursing Students, Kristen N. Oakley, Linda Carman Copel, Jennifer Gunberg Ross Jul 2026

Secondary Traumatic Stress In Baccalaureate Nursing Students, Kristen N. Oakley, Linda Carman Copel, Jennifer Gunberg Ross

International Nursing Research Congress (INRC)

Nursing students undergoing clinical training are at risk of developing negative physical and psychological secondary traumatic stress (STS) symptoms. However, the study of STS in this population has been limited by both a lack of clarity on the concept of STS and the lack of generalizable findings. Therefore, the purpose of this multi-method study was to determine the prevalence, severity, and experience of STS in traditional, non-accelerated prelicensure baccalaureate nursing students in the United States. The relationship between STS and personal and clinical learning environment factors was also explored. A descriptive, correlational multi-method survey design was used. Instruments included a …


Co-Designing New Website Ideas In The Age Of Ai, Rami Huu Nguyen Jul 2026

Co-Designing New Website Ideas In The Age Of Ai, Rami Huu Nguyen

Paul English Applied Artificial Intelligence (AI) Institute Publications

This workshop introduced students to the principles of website co-design in the age of artificial intelligence through a collaborative, project-based learning experience. Participants first explored the mission of the Paul English Applied Artificial Intelligence Institute and the instructor's career journey before learning the fundamentals of e-commerce website design. Students were organized into five teams to develop original website concepts in areas including travel, fashion, sports, and gaming. Each team conducted research using search engines and generative AI tools, including ChatGPT, Gemini, Claude, and Perplexity, created website wireframes, and presented their ideas using Canva. The workshop emphasized teamwork, creativity, user-centered design, …


Donald Trump’S Latest Tariffs Are Part Of A New Trade Strategy. The U.S. Has A Much Bigger Target Than Our Wine And Cheese, Barry Appleton Jul 2026

Donald Trump’S Latest Tariffs Are Part Of A New Trade Strategy. The U.S. Has A Much Bigger Target Than Our Wine And Cheese, Barry Appleton

Other Publications

No abstract provided.


Principles Of Sociopolitical Competency In Psychological Practice, Research, And Teaching, Richard E. Redding, Nina C. Silander Jul 2026

Principles Of Sociopolitical Competency In Psychological Practice, Research, And Teaching, Richard E. Redding, Nina C. Silander

Psychology Faculty Articles and Research

Research shows that sociopolitical attitudes and values (SPAVs) often play an important role in people’s personal and social identity, life choices, psychological and behavioral functioning, and interpersonal relationships (including those between psychologists and their clients). Thus, SPAVs are frequently relevant to the work that psychologists do, whether as clinicians, researchers, or educators. After reviewing the research on the impact of SPAVs on people’s lives and relationships, we provide a set of evidence-based principles for effectively addressing SPAVs in clinical practice, psychological research, education and professional training, and professional ethics.


Innovations In Educational Leadership To Improve Nursing Student Attendance And Engagement, Ritin Santiago Fernandez, Eleni Kata Jul 2026

Innovations In Educational Leadership To Improve Nursing Student Attendance And Engagement, Ritin Santiago Fernandez, Eleni Kata

International Nursing Research Congress (INRC)

Background: Nurse education is adapting to an evolving healthcare landscape marked by technological advances, resource constraints, and shifting patient demographics (1) Absenteeism among nursing students in classroom and clinical settings poses significant challenges to academic performance, clinical competency, and program completion (2,3). Effective leadership and advances in education are essential to identify underlying causes and implement strategies that foster students’ engagement to improve their learning outcomes thus preparing a competent healthcare workforce (4).

Aim: This scoping review aimed to map the literature on absenteeism among undergraduate nursing students, highlighting leadership and advances in education. Objectives included: (a) identifying factors contributing …


Debunking Toxic Social Media Bias With The Implementation Of Evidence-Based Nursing Education, Brittany Nettles, Julie Baker-Townsend Jul 2026

Debunking Toxic Social Media Bias With The Implementation Of Evidence-Based Nursing Education, Brittany Nettles, Julie Baker-Townsend

International Nursing Research Congress (INRC)

Social media has become a powerful influence on student nurses in the United States, shaping both their personal values and professional behaviors. Its impact is multifaceted, with evidence pointing to both positive and negative effects, particularly in relationship to nursing student empathy (Nihayati et al., 2025; Tan, Ni, Liu, & Shorey, 2024). Empathy, which is to understand and value another person’s experiences, is the cornerstone of nursing practice and remains central to ethical patient care, particularly in relation to the principles of beneficence, nonmaleficence, autonomy, justice, fidelity, and dignity. In 2024, a new ideology emerged on social media platforms: “Toxic …


Arrythmias In Autoimmune Diseases: Immune-Mediated Mechanisms And Management, Kamala P. Tamirisa, Jorge A. Irizarry-Caro, Ian Curnutt, Devika Adusumilli, Mia Jose, Meenakshi Jolly, Estelle Torbey, Annabelle S. Volgman Jul 2026

Arrythmias In Autoimmune Diseases: Immune-Mediated Mechanisms And Management, Kamala P. Tamirisa, Jorge A. Irizarry-Caro, Ian Curnutt, Devika Adusumilli, Mia Jose, Meenakshi Jolly, Estelle Torbey, Annabelle S. Volgman

Student Publications

Autoimmune diseases substantially increase the risk of atrial and ventricular arrhythmias and sudden cardiac death through shared immune-mediated mechanisms. Pro-inflammatory cytokines, autoantibodies, and progressive myocardial fibrosis disrupt ion channel function, impair conduction, and create re-entrant substrates. Across systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and systemic sclerosis (SSc), these pathways manifest as atrial fibrillation, ventricular arrhythmias, conduction disease, and heightened arrhythmic mortality. Anti Ro/SSA antibodies, in particular, contribute to QT prolongation and atrioventricular (AV) block, while cytokines such as TNF α, IL 1β, and IL 6 remodel electrophysiological properties and promote fibrosis. Advanced cardiac imaging, especially cardiac magnetic resonance (CMR), …


Staying Relevant: Radio’S Shift Toward Gen Z’S Listening Habits, Rocky Prasetyo Jati Jul 2026

Staying Relevant: Radio’S Shift Toward Gen Z’S Listening Habits, Rocky Prasetyo Jati

JURNAL KOMUNIKASI INDONESIA

The digital transformation of media has reshaped audio consumption and challenged traditional radio to stay relevant, particularly among Generation Z, the digital-native cohort. This article examines how community and hyperlocal radio stations are adapting to shifting media dynamics by combining conventional broadcasting with digital innovations. The study employs qualitative methods. As Gen Z prefers flexible, personalized, and on-demand content, radio stations are repositioning broadcasters as multi-platform content creators and online community managers. Adaptation strategies include integrating radio with visually oriented social media platforms, offering curated playlists, and adopting participatory models such as digital tipping (saweran). Radio stations are …


Between Tradition And Profession: Intersectionality Of Javanese Women Journalists In Surakarta’S Newsroom, Rahman Hakim, Vanggi Eka Riski Jul 2026

Between Tradition And Profession: Intersectionality Of Javanese Women Journalists In Surakarta’S Newsroom, Rahman Hakim, Vanggi Eka Riski

JURNAL KOMUNIKASI INDONESIA

Javanese female journalists in Surakarta navigate the simultaneous pressures of professional journalism and deeply internalised Javanese cultural values such as nrimo (acceptance of one’s circumstances), andhap asor (humble and deferential conduct), and tepa selira (attentiveness to the feeling of others). These values shape their everyday conduct in the newsroom yet often remain unacknowledged in existing literature. Combining Intersectionality Theory, Face-Negotiation Theory (Ting-Toomey), and Self Theory (Rogers) as complementary frameworks, this qualitative study adopts a constructivist paradigm and employ phenomenological method to explore how female journalists make sense of the intersection between gender norms and local cultural values. Data were collected …


Stakeholder Perceptions Of Artificial Intelligence In The Indonesian Film Industry, Louisa Christine Hartanto, Patrisia Amanda Pascarina, Cosmas Gatot Haryono Jul 2026

Stakeholder Perceptions Of Artificial Intelligence In The Indonesian Film Industry, Louisa Christine Hartanto, Patrisia Amanda Pascarina, Cosmas Gatot Haryono

JURNAL KOMUNIKASI INDONESIA

This study explores how stakeholders in the Indonesian film industry perceive the emergence and use of artificial intelligence (AI). Framed by Roger's Diffusion of Innovation theory, this study explores how artificial intelligence (AI) is positioned as an emergent technological innovation, spreading via communication channels across the film industry's social ecosystem. This approach illuminates how distinct categories of adopters collectively construct the meaning of AI and shape their varied responses to its encroachment on creative labour. Employing a qualitative descriptive approach, the study draws on data from two Focus Group Discussions involving directors, actors, government experts, academics, emerging filmmakers, students, and …


Cultural Traits May Replace Human Mobility Data In Forecasting Covid-19 Mortality: A Deep Learning Approach, Saif Abbas, Tamer Oraby, Michael G. Tyshenko, Samit Bhattacharyya Jul 2026

Cultural Traits May Replace Human Mobility Data In Forecasting Covid-19 Mortality: A Deep Learning Approach, Saif Abbas, Tamer Oraby, Michael G. Tyshenko, Samit Bhattacharyya

School of Mathematical & Statistical Sciences Faculty Publications

The COVID-19 pandemic highlighted the need for accurate epidemic forecasting to support public health decision-making. Most existing approaches depend heavily on human mobility data, while largely neglecting population behavior shaped by socio-cultural norms. In this study, we analyze daily COVID-19 mortality and Google mobility data from 72 countries during the first 130 d of the pandemic, a period characterized by high uncertainty and behavioral heterogeneity. In particular, we examine whether Hofstede’s country-level cultural dimensions can serve as latent behavioral forecasters of mortality in lieu of dynamic mobility indicators. Using 100 d for training and 30 d for forecasting, we employ …


20260722: Academic Affairs, 1994-1991, Academic Affairs Jul 2026

20260722: Academic Affairs, 1994-1991, Academic Affairs

Guides to University Archives

These items include materials from the office of Academic Affairs at Marshall University from 1984-1991. Items were received in the early 1990s and include Marshall University Grants Reports for FY 1988-1989, 1990, and 1991. Additionally included are grant reports from 1984-1985, and some responsibilities and procedures for grant applications. This is not an exhaustive list. Please download the finding aid for a full list of contents.


Rosin V. Graso Jul 2026

Rosin V. Graso

All Decisions

A landlord commenced a Supreme Court breach of contract action for unpaid rent against an 80-year-old bedbound rent-stabilized tenant, obtained an ex parte TRO ordering immediate payment, and then moved for ejectment when the tenant did not comply — all before the first court appearance. The court denied ejectment as a drastic remedy, citing the landlord's erroneous rental amount, the tenant's medical hardship, a habitability dispute, and the tenant's willingness to tender rent. The court vacated the TRO, permitted the tenant to interpose a late answer, and declined to transfer the proceeding to Housing Court.


Belmont Holding Llc V. Corporan Jul 2026

Belmont Holding Llc V. Corporan

All Decisions

In this nonpayment proceeding, Respondent moved to dismiss under CPLR § 3211 on the ground that the rent ledger included a lump sum of $4,665.28 in undated 'arrears,' rendering the termination notice defective. The court granted the motion, holding that inclusion of the lump sum without identifying the periods it covered was a non-amendable defect requiring dismissal.


Accurate 3d Computational Modeling Of Multiphase Flows In Boiling Heat Transfer, Winston O. James Iii Jul 2026

Accurate 3d Computational Modeling Of Multiphase Flows In Boiling Heat Transfer, Winston O. James Iii

Theses

In the past ten years, the electronics industry has seen a sustained demand to reduce thermal resistances while increasing volumetric heat removal. The limitations of traditional air-cooled systems have caused a major industry shift toward liquid cooling. To maintain beam quality, high output efficiency, and continuous uptime, high-powered lasers require cooling to manage the waste heat of 5-55 kW. Small modular nuclear reactors (SMRs) have a capacity of 350 MW or less per unit and operate at an efficiency of 33.4% to 45%. As a result, 55% to 66% of the heat generated must be dissipated. Within data centers, a …


Exploring School Counselors’ Use Of Community Cultural Wealth To Support Latine Students’ Stem Aspirations: An Interpretative Phenomenological Approach, Valerie Leija, Eunice Lerma, Javier Cavazos Vela Jul 2026

Exploring School Counselors’ Use Of Community Cultural Wealth To Support Latine Students’ Stem Aspirations: An Interpretative Phenomenological Approach, Valerie Leija, Eunice Lerma, Javier Cavazos Vela

Human Development and School Services Faculty Publications

Despite growing diversity across many professions, Latine individuals remain underrepresented in the STEM workforce. School counselors are uniquely positioned to help eliminate these disparities by empowering Latine students to pursue STEM career pathways. Therefore, the purpose of this qualitative study was to explore how school counselors capitalize on Latine students’ community cultural wealth to support them in pursuing postsecondary STEM opportunities. We conducted 50-90-minute interviews with eight school counselors from South Texas to explore their lived experiences supporting Latine students with STEM aspirations. Interview transcripts were analyzed using interpretative phenomenological analysis. The following themes emerged: (a) linguistically responsive practices, (b) …


Administrative Appeal Decision - Genyard, Jasper (2026-06-30) Jul 2026

Administrative Appeal Decision - Genyard, Jasper (2026-06-30)

Parole Administrative Appeal Decisions

No abstract provided.


Global Time-Resolved Measurements Of Inlet/Isolator Unstart Induced By Mass Injection, Andrew N. Bustard, Benjamin L. Bemis, Aaron Marques, Matthew J. Zahr, Thomas J. Juliano Jul 2026

Global Time-Resolved Measurements Of Inlet/Isolator Unstart Induced By Mass Injection, Andrew N. Bustard, Benjamin L. Bemis, Aaron Marques, Matthew J. Zahr, Thomas J. Juliano

Publications

The inner surface pressure of an axisymmetric inlet/isolator model was measured using anodized-aluminum pressure-sensitive paint (PSP) viewing through cast acrylic. Temperaturesensitive paint was utilized to correct for the PSP’s temperature sensitivity. The model was tested under Mach 5.7 flow at 𝑹𝒆 = 7.1 ×106 /m under conventional noise conditions. Transverse jet injection with jet-to-inlet mass-flow ratios up to 0.8 was used to induce unstart in the inlet/isolator. Background-oriented schlieren visualization of the inlet shocks was collected simultaneously with the PSP to determine when the inlet unstarted. Computational fluid dynamics results were used to determine off-wall flow structures and quantify approach …


Getting Tolkien Right: Middle-Earth In The National Review, Craig Franson Jul 2026

Getting Tolkien Right: Middle-Earth In The National Review, Craig Franson

Journal of Tolkien Research

J.R.R. Tolkien’s The Lord of the Rings entered Anglo-American popular consciousness as an emblem of 1960s, leftist youth culture, but the novel slowly gained currency across the political right. In the U.S., conservative intellectuals published book notes and articles on Tolkien’s writing in their movement’s leading journals, gradually habituating their readers to seeing the once-obscure English fantasist as a canonical writer for American conservatism. The most prominent of these journals was William F. Buckley’s National Review, which recurrently promoted Tolkien’s writing and offered a distinctly partisan interpretation of his texts. This paper offers a partial outline of the conservative …


The Edge-Distinguishing Game, Nathaniel Benjamin, Elisa Benthem, Cooper Burkel, Marissa E. Chesser, Mike Janssen Jul 2026

The Edge-Distinguishing Game, Nathaniel Benjamin, Elisa Benthem, Cooper Burkel, Marissa E. Chesser, Mike Janssen

Communications on Number Theory and Combinatorial Theory

In this paper, we introduce a graph coloring game called the Edge-Distinguishing Game (EDGe). The edge-distinguishing chromatic number of a graph is used to determine the moves each player can make. We determine which player has a winning strategy for particular graphs and graph families. Additionally, utilizing principles from game theory as well as previous work on a computational solution for the Game of Cycles.


Missed Diagnoses Beyond Endemic Borders: How Knowledge And Resource Access Shape Testing For Cutaneous Leishmaniasis And Chagas Disease, Alyssa Agnew, Brandon Lopez, Cherry Mawii, Erin Aslan, Sreshta Sankar, Semené Clement Jul 2026

Missed Diagnoses Beyond Endemic Borders: How Knowledge And Resource Access Shape Testing For Cutaneous Leishmaniasis And Chagas Disease, Alyssa Agnew, Brandon Lopez, Cherry Mawii, Erin Aslan, Sreshta Sankar, Semené Clement

OneUSF Undergraduate Research Conference

Cutaneous Leishmaniasis (CL) and Chagas Disease (CD) are both tropical vector borne diseases most common in endemic regions. CL is caused by a parasite spread through sandfly bites. CD is caused by the parasite transmitted through the feces of Triatomine bugs. They are not common in non-endemic regions resulting in lack of training and knowledge required to properly diagnose and treat these diseases. This study assesses how clinician knowledge and access to diagnostic tools influence testing practices for CL and CD in non-endemic regions and contribute to diagnostic bias, underrecognition, and delayed diagnosis.

A comprehensive literature review was performed through …


Leveraging High School Students Knowledge Of Obesity Conundrum, Lauren R. Mcconnell, Nalani N. Galindez, Lauren R. Selent Jul 2026

Leveraging High School Students Knowledge Of Obesity Conundrum, Lauren R. Mcconnell, Nalani N. Galindez, Lauren R. Selent

OneUSF Undergraduate Research Conference

Adolescent obesity, defined as a BMI at or above the 95th percentile for age and sex, affects approximately 21% of US adolescents aged 12 to younger than 18 years. This chronic, progressive disease is associated with significant immediate and long-term health consequences and requires comprehensive, intensive treatment rather than "watchful waiting.Changes in diet, including decreased fast food and soda consumption are necessary to obesity reduction. The purpose of this study is to examine high school students knowledge and attitudes regarding obesity. A quantitative, causal-comparative design was used to identify differences across groups. The independent variable was the grade level of …


Leveraging Compliance With Infection Control Policies Among Healthcare Workers In A Multi-Hospital System, Lauren R. Mcconnell, Ethan C. Anthony, Lauren R. Selent Jul 2026

Leveraging Compliance With Infection Control Policies Among Healthcare Workers In A Multi-Hospital System, Lauren R. Mcconnell, Ethan C. Anthony, Lauren R. Selent

OneUSF Undergraduate Research Conference

Despite the national push, healthcare workers compliance with Standard Precautions (SP) and Transmission-Based Precautions show significant gaps in both understanding and implementation.1 The conundrum of hospital nosocomial infections obfuscates the threat to patient safety.2 The purpose of this study is to examine staff compliance with infection control policies by infection type within a multi-hospital system. A quantitative, causal-comparative design was used to identify differences across groups. The independent variable was the ‘type of isolation’ assigned by the infection control practitioner. The dependent variables were staff adherence to the infection control policies including: 1) Isolation sign on patient’s door ; 2) …


Patient Perspectives On Lung Cancer Screening Uptake: Findings From In-Depth Interviews, Patricia A. Falcon Jul 2026

Patient Perspectives On Lung Cancer Screening Uptake: Findings From In-Depth Interviews, Patricia A. Falcon

OneUSF Undergraduate Research Conference

Lung cancer remains the leading cause of cancer death in the United States and is primarily caused by tobacco use. Lung cancer screening with a Low-Dose Computed Tomography (LDCT) scan is an established method to detect this disease at an early stage and can reduce its death rate by up to 20%. However, the uptake of lung cancer screening across eligible individuals has been low, especially among underserved populations. The goal of this study was to have a better understanding of how to increase lung cancer screening among eligible underserved population. Eligible participants included adults aged 50 to 80 years …


Deutsche Musik: Exploring German Identity Through Music, Selah A. Thompson Jul 2026

Deutsche Musik: Exploring German Identity Through Music, Selah A. Thompson

OneUSF Undergraduate Research Conference

While Germany is recognized as the fourth-largest music market in the world,music has a complex role within the construction of German national andcultural identity. Tracing musical history back to the 12th century with theMinnesinger, to the great classical composers like Bach and Beethoven andinto the 20th and 21st centuries with German heavy metal groups like theScorpions, Germany's historical roots are heavily intertwined with music.Many historical events in Germany have featured music as a prominentcatalyst, such as the Swing counterculture during the Nazi regime to theresilience of the East German pop scene. This study traces the complex andrich history of music …


German Involvement In The European Space Agency, Amanda M. Chesney, Abbigail Geiger Jul 2026

German Involvement In The European Space Agency, Amanda M. Chesney, Abbigail Geiger

OneUSF Undergraduate Research Conference

As our technological advancements expand, so does our knowledge of the universe beyond Earth. And as technology becomes increasingly more accessible, more countries are becoming involved in space exploration and research. Germany, in particular, is a global leader in technological innovation and engineering—does this still apply to the field of space in particular? How much of recent innovation in space research has Germany been involved in? To answer this question, this study examined Germany’s contributions to the European Space Agency’s efforts in space research and exploration. Information was sourced from the European Space Agency’s recent projects. The study observed these …


Leveraging The Patient Experience: Satisfaction With Hospital Food Services, Daniel Hansen Harwin, Lauren Rae Selent, Lauren Reilly Mcconnel Jul 2026

Leveraging The Patient Experience: Satisfaction With Hospital Food Services, Daniel Hansen Harwin, Lauren Rae Selent, Lauren Reilly Mcconnel

OneUSF Undergraduate Research Conference

Patients want and expect better health care services than they did in the past. Within hospitals, consumption of proper nutrition is vital. Patients and nutrition share a unique relationship that is essential for health, this connection is complex including important variables such as food quality. This study examined how patients’ satisfaction with food differed by clinical units. Initially, a MANOVA was conducted. For significant findings ANOVA contrasts were conducted on each dependent variable. The independent variables are the individual clinical units to which the patients were admitted. The dependent variables were the patients' responses to survey questions including: 1) There …


Validation Of A Remote Collection Device For Human Milk Composition Analysis, Thi Yen Nhi Nguyen, Ryan Pace Jul 2026

Validation Of A Remote Collection Device For Human Milk Composition Analysis, Thi Yen Nhi Nguyen, Ryan Pace

OneUSF Undergraduate Research Conference

Human milk (HM) composition demonstrates remarkable variation across stage of lactation, time of day, and environmental exposures such as diet. Most research protocols require in-person collection and immediate freezing to maintain sample integrity, resources often unavailable in remote or low-resource settings. Validated alternative collection and storage protocols are needed. This study evaluated whether HemaSpot HF (HF), a “next-generation” dry blood spot device, is suitable for remote HM collection and protein analysis. HM was applied to HF devices and protein concentrations were measured using bicinchoninic acid assays. We assessed protein concentration stability across storage durations, elution times, and sample volumes. We …


How Do Different Receptor Targets In Glioblastoma Differentially Shape The Tumor Microenvironment And Influence Tumor Progression And Therapeutic Response?, Vedant A. Patel, Rohit V. Borra, Andrew P. Thomas, Emily Berger Jul 2026

How Do Different Receptor Targets In Glioblastoma Differentially Shape The Tumor Microenvironment And Influence Tumor Progression And Therapeutic Response?, Vedant A. Patel, Rohit V. Borra, Andrew P. Thomas, Emily Berger

OneUSF Undergraduate Research Conference

Glioblastoma (GBM) is one of the most aggressive brain tumors, with a median survival of approximately 15 months despite treatment and a recurrence rate near 90%. Therapeutic failure is largely driven by an immunosuppressive and therapy-resistant tumor microenvironment (TME). GBM progression depends on dysregulated receptor-mediated signaling pathways that regulate angiogenesis, immune evasion, stemness, metabolic adaptation, and phenotypic transition. Although targeting individual receptors has been explored, outcomes remain limited. This literature review evaluates how targeting receptor-mediated pathways—including neuromodulatory targets such as mGluR3—affects the TME and therapeutic response. It also compares the effectiveness of single versus multi-receptor targeting strategies. A PubMed search …


The Aging Muscle Phenotype Of C57bl/6j Wild-Type Mice, Gaurav Shingote Jul 2026

The Aging Muscle Phenotype Of C57bl/6j Wild-Type Mice, Gaurav Shingote

OneUSF Undergraduate Research Conference

Aging causes a slow and progressive loss of muscle fiber number and size. Sarcopenia is termed as an age-related loss of muscle mass and function. Here, we characterized the aging skeletal muscle phenotype of C57Bl/6J wildtype mice. 30 weeks old (young) and 2 years old (aged) C57Bl/6J wildtype male mice were utilized in this study. The body weight and hind leg muscle weight were recorded during collection. The hind leg muscle Tibialis anterior (TA) was OCT embedded, cut at the mid- belly region, and stained with hematoxylin and eosin (H&E) stain as per our previously published protocol. The 20X images …