Digital Commons Network^™

Lymphodepletion, Tumor-Infiltrating Lymphocytes, And High Versus Low Dose Il-2 Followed By Pembrolizumab In Patients With Metastatic Melanoma, Merve Hasanov, Simin Kiany, Marie-Andrée Forget, Roland Bassett, Michael A Davies, Adi Diab, Jeffrey E Gershenwald, Isabella C Glitza, Jeffrey E Lee, Anthony Lucci, Jennifer L Mcquade, Sapna P Patel, Merrick I Ross, Hussein A Tawbi, Jennifer A Wargo, Michael K Wong, Chantale Bernatchez, Patrick Hwu, Cara Haymaker, Rodabe N Amaria

Faculty, Staff and Student Publications

This study evaluated the efficacy and safety of unengineered tumor-infiltrating lymphocytes (TILs) combined with pembrolizumab and either high (HD, Arm-1) or low (LD, Arm-2) doses of IL-2 in patients with metastatic melanoma (MM). Patients were lymphodepleted with cyclophosphamide and fludarabine, followed by TIL infusion and IL-2 (Arm-1: 720,000 IU/kg IV q 8 hrs up to 15 doses; Arm-2: 2 million IU SC for 14 days). Patients received pembrolizumab 200 mg IV starting 21 days post-TIL infusion, and every 3 weeks for up to 2 years. The primary endpoint was overall response rate (ORR) per RECIST 1.1. Blood samples were collected …

The Kiva System Versus Balloon Kyphoplasty For Vertebral Compression Fracture: A Meta-Analysis Of Randomized Control Trials, Humaid Al Farii, Nikhil Gattu, Caleb M Yeung, Christopher A Alvarez-Breckenridge, Robert Y North, Claudio E Tatsui, Laurence D Rhines, Valerae O Lewis, Justin E Bird, Shalin S Patel

Faculty, Staff and Student Publications

Background: Vertebral compression fractures (VCFs) are the most common type of vertebral body fracture. The Kiva VCF Treatment System is a relatively novel technique to manage VCFs. The aim of this study was to compare the efficacy of Kiva versus standard Balloon Kyphoplasty (BK) through evaluation of published randomized controlled trials (RCTs).

Methods: This study was performed following the guidelines for PRISMA. We performed a systematic literature search using PubMed and MEDLINE in June 2023. The search keywords were "Kiva" and "Kyphoplasty" which yielded a total of 112 articles. Outcome measures included pain, measured through the Visual Analogue Scale (VAS) …

The Role Of Neutrophils In Vasospasm And Delayed Cerebral Ischemia After Aneurysmal Subarachnoid Hemorrhage: Is It Time For A Sah Clinical Trial Targeting Neutrophils?, William W Wroe, Hussein A Zeineddine, Spiros L Blackburn, Jaroslaw Aronowski, Devin W Mcbride

Faculty, Staff and Student Publications

Aneurysmal subarachnoid hemorrhage (aSAH) is a devastating neurological disease, and one of the primary drivers of morbidity after aneurysm rupture is the phenomenon of delayed cerebral ischemia (DCI). Significant knowledge has been gained over the past two decades of the impact of neuroinflammation in DCI; and neutrophils are now believed to play a major role. There is significant human subject data showing the rise of neutrophil related inflammatory markers and neutrophil's association with poor outcome after aSAH, but as of yet no trials involving human subjects have been done specifically targeting neutrophils. There is however a growing body of evidence …

Sars-Cov-2 Rna-Binding Protein Suppresses Extracellular Mirna Release, Hyejin Mun, Chang Hoon Shin, Qingxuan Fei, Andrea Estefania Lopez Giraldo, Kyoung-Min Choi, Ji Won Lee, Kyungmin Kim, Kyung-Won Min, Leilei Shi, Mark T Bedford, Dong-Chan Kim, Yoo Lim Chun, Seonghyun Ryu, Dongin Kim, Jeong Ho Chang, Ryan T Westrope, Michelle Shay, Edward Nguyen, Junho K Hur, Abigail Agyenda, Nam Chul Kim, Sung-Ung Kang, Woonghee Lee, Je-Hyun Yoon

Faculty, Staff and Student Publications

SARS-CoV-2 is the betacoronavirus causing the COVID-19 pandemic. Although the SARS-CoV-2 genome and transcriptome were reported previously, the function of individual viral proteins is largely unknown. Utilizing biochemical and molecular biology methods, we identified that four SARS-CoV-2 RNA-binding proteins (RBPs) regulate the host RNA metabolism by direct interaction with mature miRNA let-7b revealed by Nuclear Magnetic Resonance spectroscopy (NMR). SARS-CoV-2 RBP Nsp9 primarily binds mature miRNA let-7b, a direct ligand of the Toll-like Receptor 7 (TLR7), one of the potential SARS-CoV-2 therapeutics. Nsp9 suppresses host gene expression possibly by promoting let-7b-mediated silencing of a cellular RNA polymerase, POLR2D. In addition, …

Normalization Of Temperature Effects For Quality Assurance Of Quantitative Prostate Apparent Diffusion Coefficient Imaging Across Multiple Sites, Ken-Pin Hwang, Joshua Yung, R Jason Stafford, Caroline Chung, Aradhana M Venkatesan

Faculty, Staff and Student Publications

Background: Apparent Diffusion Coefficient (ADC) as measured by diffusion weighted imaging is known to negatively correlate with prostate tumor aggressiveness. Heterogeneity in system and protocol performance causes potential variability in ADC acquired across a large scanner network, prompting a need to evaluate quantitative ADC from a prostate-specific MR diffusion protocol as part of quality assurance (QA). Due to the temperature dependence of ADC, repeatability and reproducibility assessments typically require phantoms to maintain a temperature of 0°C, imposing a considerable burden when assessing large numbers of scanners.

Purpose: To develop a QA procedure at room temperature for assessing the reproducibility of …

Addressing Controversy In Fusobacterium Nomenclature: What Exactly Does “F Nucleatum” Refer To?, Martha A Zepeda-Rivera, Floyd E Dewhirst, Susan Bullman, Christopher D Johnston

Faculty, Staff and Student Publications

The F. nucleatum group (sensu lato) is historically composed of four subspecies (F. nucleatum subsp. animalis, F. nucleatum subsp. nucleatum, F. nucleatum subsp. polymorphum, F. nucleatum subsp. vincentii). Although F. nucleatum sensu lato members are typically associated with oral disease, they have recently been shown to disseminate to the gastrointestinal tract and are associated with adverse health conditions such as inflammatory bowel disease and colorectal cancer (CRC). A growing debate over the nomenclature applied to Fusobacterium taxonomy has resulted in different names for these lineages, shifting them from their historic subspecies designations to …

Fusobacterium Sphaericum Sp Nov, Isolated From A Human Colon Tumor Adheres To Colonic Epithelial Cells And Induces Il-8 Secretion, Martha A Zepeda-Rivera, Yannick Eisele, Alexander Baryiames, Hanrui Wu, Claudia Mengoni, Gianmarco Piccinno, Elsa F Mcmahon, Kaitlyn D Lacourse, Dakota S Jones, Hans Hauner, Samuel S Minot, Nicola Segata, Floyd E Dewhirst, Christopher D Johnston, Susan Bullman

Faculty, Staff and Student Publications

Cancerous tissue is a largely unexplored microbial niche that provides a unique environment for the colonization and growth of specific bacterial communities, and with it, the opportunity to identify novel bacterial species. Here, we report distinct features of a novel Fusobacterium species, F. sphaericum sp. nov. (Fs), isolated from primary colon adenocarcinoma tissue. We acquire the complete closed genome and associated methylome of this organism and phylogenetically confirm its classification into the Fusobacterium genus, with F. perfoetens as its closest neighbor. Fs is phenotypically and genetically distinct, with morphological analysis revealing its coccoid shape, that while similar to …

A Review Of Engraftment Assessments Following Fecal Microbiota Transplant, Chloe Herman, Bridget M Barker, Thais F Bartelli, Vidhi Chandra, Rosa Krajmalnik-Brown, Mary Jewell, Le Li, Chen Liao, Florencia Mcallister, Khemlal Nirmalkar, Joao B Xavier, J Gregory Caporaso

Faculty, Staff and Student Publications

Fecal Microbiota Transplant (FMT) is a treatment for recurrent Clostridium difficile infections and is being explored for other clinical applications, from alleviating digestive and neurological disorders, to restoring microbiomes impacted by cancer treatment. Quantifying the extent of engraftment following an FMT is important in understanding a recipient's response to treatment. Engraftment and clinical response need to be investigated independently to evaluate an FMT's role (or lack thereof) in achieving a clinical response. Standardized bioinformatics methodologies for quantifying engraftment extent would not only improve assessment and understanding of FMT outcomes, but also facilitate comparison of FMT results and protocols across studies. …

Piezoelectric Energy Harvesting From Roadways: Challenges, Advances, And Future Directions, Heba Mohamed Gaber, Mohamed Abdel-Raheem

Civil Engineering Faculty Publications

As the global demand for renewable energy intensifies, piezoelectric energy harvesting from roadways has emerged as a promising avenue for sustainable power generation. This systematic literature review analyzes 61 peer-reviewed studies to assess the feasibility, performance, and potential of integrating piezoelectric systems into roadway infrastructure. While technology faces challenges, such as high installation costs, limited energy output, and a scarcity of thorough economic evaluations, findings suggest it holds considerable promise as a supplementary renewable energy source. The review analyzes the operational characteristics and efficiencies of various piezoelectric transducers, identifies key factors influencing system performance, and evaluates recent technological advances. It …

The Equity Implications Of Pecuniary Externalities On An Electric Grid, Charles Sims, Gasser G. Ali, J Scott Holladay, Tim Roberson, Chien-Fei Chen, Islam H. El-Haddad

Civil Engineering Faculty Publications

The adoption of rooftop photovoltaic (PV) systems can create upward pressure on retail electricity rates as utilities are forced to spread their fixed costs of generation and transmission across a smaller customer base. Since high-income households are more likely to purchase PV systems, low-income households may be disproportionately impacted by these rate increases. Using a novel combination of agent-based computational economic modeling and a choice experiment of rooftop solar adoption, we show how this pecuniary externality between low- and high-income customers increases low-income electricity bills by 10% in an area with some of the highest poverty rates in the United …

Advancements In Sinkhole Remediation: Field Data-Driven Sinkhole Grout Volume Prediction Model Via Machine Learning-Based Regression Analysis, Bubryur Kim, Yuvaraj Natarajan, K. R. Sri Preethaa, V. Danushkumar, Ryan Shamet, Jiannan Chen, Rui Xie, Timothy Copeland, Boo Hyun Nam, Jinwoo An

Civil Engineering Faculty Publications

Sinkhole formation poses a significant geohazard in karst regions, where unpredictable subsurface erosion often necessitates costly grouting for stabilization. Accurate estimation of grout volume remains a persistent challenge due to spatial variability, site-specific conditions, and the limitations of traditional empirical methods. This study introduces a novel machine learning-based regression model for grout volume prediction that integrates cone penetration test (CPT)-derived Sinkhole Resistance Ratio (SRR) values, spatial correlations between CPT and grouting points (GPs), and field-recorded grout volumes from six sinkhole sites in Florida. Three data transformation methods, the Proximal Allocation Method (PAM), the Equitable Distribution Method (EDM), and the Threshold-based …

Seasons Of Change, Danielle Wyenberg

Pro Rege

No abstract provided.

The Antirevolutionary: Life And Works Of Groen Van Prinsterer (Book Review), Keith C. Sewell

Pro Rege

Reviewed Title: The Antirevolutionary: Life and Works of Groen van Prinsterer by Roel Kuiper. Eugene, OR: Pickwick Publications, 2025. Translated by Harry Van Dyke. ISBN: 9798385224562.

A Prayer For The Needs Of The World, Brad Hickey

Pro Rege

No abstract provided.

Little Did I Know, Christopher V. Hollister

Communications in Information Literacy

Christopher Hollister, co-founder and co-editor-in-chief, reflects on his time with CIL in this farewell editorial.

Searching For Change: Exploring Historical Silences And Counterstorytelling Through Youth-Led Social Justice Movements, Ariana Varela

Communications in Information Literacy

During a first-year engagement program, a librarian and a cohort of students worked towards a shared understanding of dominant narratives, historical silences, and creating counterstories through research to center marginalized stories and complicate existing narratives. As information literacy professionals, we should design interactive lessons that demonstrate critical search and evaluation skills through topics that are relevant to students and encourage them to work with issues that impact their communities. Incorporating critical information literacy, feminist pedagogy, and critical race theory, this two-day micro-seminar introduced first-year students to the legacy of youth community activism in the neighborhoods around their university. They practiced …

Privacy-By-Design: Case Studies In Interactive Record Linkage Using A Hybrid Human-Computer System, Hye-Chung Kum, Eric Ragan, Mahin Ramezani, Gurudev Ilangovan, Theodoros Giannouchos, Qinbo Li, Adam D'Souza, Elmer V Bernstam, Jeffrey R Curtis, Alva O Ferdinand, Cason Schmit

Faculty, Staff and Student Publications

Objective: High-quality patient matching from several sources without a common identifier (ID) requires interactive record linkage (RL) using a hybrid human-computer system. MiNDFIRL (MInimum Necessary Disclosure For Interactive Record Linkage) is a hybrid prototype software system that facilitates maximizing linkage accuracy while minimizing information disclosure. We present and evaluate MiNDFIRL using two real-world case studies.

Materials and methods: Two user studies were conducted linking 10,000 data pairs from EHR data and 18,240 unique patient IDs from patient generated data. After automated RL, manual review was conducted by three teams of four reviewers (12 total) using MiNDFIRL to resolve potential matches …

Communication And Privacy Management Between Female Veterans, Their Military Voluntary Kin, And Biolegal Family Members, Carol Lee Tschampl-Diesing

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Military voluntary kin (MVK) refer to relationships between Service members and/or Veterans that they consider to be “like family,” despite lacking blood or legal ties. The purpose of this qualitative/interpretive communication study was to explore how female Veterans, who are the largest growing population of U.S. Veterans, discursively construct MVK relationships, navigate communication, privacy management, and boundary turbulence with their MVK and biolegal families (BLF). The researcher undertook in-depth interviews with nine female U.S. Veterans who reported having at least one MVK. The researcher posed four research questions, engaging in an interpretive analysis.

Participants described three ways they navigated interactions …

Eyewitness Consent To Be Video Recorded: Implications For Identification Accuracy And Confidence, Sarah Argüelles

Student Theses

Recently, a recommendation has emerged for law enforcement to record the full eyewitness identification procedure, recognizing the critical role eyewitness testimony plays in the justice system (Wells et al., 2020). However, in some states, such as New York, law enforcement is required to seek consent from witnesses before recording (Municipal Police Training Council & Division of Criminal Justice Services, 2017). Since this practice is relatively new, there has been limited research on how obtaining consent may affect eyewitness accuracy. It was hypothesized that obtaining consent to video record could be linked to a witness’s confidence in their identification, with higher …

A Contemporary Guide To An International Aortic Super-Fellowship For Surgical Trainees And Surgeons In The United States, Roberto G. Aru, Adam W. Beck, Stéphan Haulon

Department of Surgery Faculty Papers

Fenestrated and branched technology for endovascular aortic repair of the thoracoabdominal aorta and aortic arch has evolved significantly over the last decade. The spectrum of these devices is largely available outside the United States (US), while patient-specific company manufactured devices are limited to ten sites in the US, with ongoing expanding use of market-approved off-the-shelf devices. The necessary environment for appropriate education to ensure safe dissemination of these technologies is limited, and the current centralization of both device availability and skillsets affects trainee education. In order to refine their endovascular treatment algorithm and planning/implantation skillsets at these multidisciplinary aortic centers, …

Impacts Of Halophila Stipulacea Invasion On Organic Matter Dynamics In Thalassia Testudinum Dominated Seagrass Meadows, Puerto Rico, Jonathan D. Herald

Master's Theses

Halophila stipulacea (Forsskål & Niebuhr) is a tropical seagrass native to the Red Sea, Persian Gulf, and Indian Ocean. In 2002, invasive populations of H. stipulacea were identified in the Caribbean Sea where it now forms large meadows, displaces native seagrasses, alters invertebrate and fish communities, and potentially affects organic matter dynamics in invaded meadows. This study investigated differences in organic matter dynamics between meadows of H. stipulacea and native Thalassia testudinum within the Jobos Bay National Estuarine Research Reserve (JBNERR) on the southern coast of Puerto Rico. From March 2023-March 2024, sampling was conducted across four sites to investigate …

Effects Of Urbanization On The Population Genetics And Morphology Of The White-Footed Mouse (Peromyscus Leucopus) Across An Urban–Rural Gradient, Sydney A. Morton

Master's Theses

Urbanization alters landscapes in ways that can reshape wildlife populations by changing habitat connectivity, resource availability, and selective pressures. To investigate how urbanization influences both genetic differentiation and morphology in small mammals, I examined Peromyscus leucopus (PELE) across an urban–rural gradient in northern Georgia. Using RADseq SNP data from PELE individuals across 13 sites, I quantified pairwise genetic differentiation (F_ST) and tested how various environmental variables, including impervious surface cover, human population density, and tree canopy cover, predict genetic differentiation after accounting for geographic distance. Genetic differentiation increased with geographic distance and, after controlling for isolation by …

Peer Mentoring And Teacher Collaboration: A Case Study Of Practices And Self-Efficacy In A Rural Title I School, Whitney Ensley

Dissertations

This qualitative case study examined how peer mentoring influences teacher self-efficacy at a rural elementary school in southeastern Georgia. Using Bandura's self-efficacy theory and Wenger's communities of practice framework, the research investigated how teachers engage in mentoring relationships and how these practices contribute to professional growth.

Semi-structured interviews and reflection journals were conducted with ten elementary teachers representing diverse experience levels (1-21+ years) and grade levels. Findings revealed that meaningful peer mentoring occurred primarily through informal, trust-based relationships within grade-level teams rather than formal programs. Teachers engaged collaboratively in lesson planning, data analysis, and problem-solving.

Results demonstrated that peer mentoring …

The Interplay Between Structure And Teacher Agency In The Detracking Of Algebra 1: A Qualitative Case Study, Kristine Norris

Dissertations

This single qualitative case study examined how detracking Algebra 1 at Mountainview High School (pseudonym) restructured the course’s rules and resources and how teachers exercised agency in response. Guided by Giddens’ structuration theory and Weis and Fine’s critical bifocality, the study explored how institutional structures both shape and are reshaped by teacher action within broader systems of inequality. Participants were seven Algebra 1 teachers involved in the first two years of detracking. Data was gathered using semi-structured interviews and analysis focused on two research questions: (1) In what ways did detracking alter the structures of Algebra 1 courses, and (2) …

Teacher Perceptions Of Culturally Relevant Pedagogy In High School Social Studies Classrooms: An Ethnographic Case Study, Sheronda L. Hardy

Dissertations

Historically, scholarly research regarding the education of Black, Indigenous, and People of Color (BIPOC) has been written through a deficit lens. Research that acknowledges positive learning experiences and outcomes is limited. This qualitative ethnographic case study aims to contribute to the growing body of social studies literature that offers insights into the need for asset-based pedagogies, such as Gloria Ladson-Billings' Culturally Relevant Pedagogy (CRP), as well as the impact that this framework has on BIPOC student populations. By exploring the perceptions of high school social studies teachers regarding CRP, teacher preparation programs, and professional development, this study of five teacher …

Imagine The Peace: Exploring The Intimacy Of Imagined Intergroup Contact In Kosovo, Eric Manley

Dissertations

Peacebuilders have utilized positive intergroup contact in various settings to help groups encounter each other. I implemented three different intimate imagined contact scenarios in Peja, Kosovo with Albanian and Serb participants. I conducted this research to understand how multiple intimate social settings could impact individuals’ encounters with the outgroup during each imagined scenario, thus gathering better operationalization efforts regarding intimacy and imagined contact. Using written responses, interviews, and one-year follow-up interviews, the café and workplace scenarios created a positive experience for most participants, and while half of participants had a positive experience with the fiancé scenario, many more had negative …

Teaching For Global Ecological Citizenship: A Case Study Of Rural Teachers’ Experiences And Practices, Sarah L. Tennyson

Dissertations

The purpose of this qualitative case study is to examine the experiences and practices of rural educators incorporating global ecological citizenship education into the social studies classroom. The systematic themes of social studies education in the past must evolve with the diversity of the classroom. Traditional themes taught must become more globally relevant, including ecological citizenship, the responsibility of citizens to sustain and participate in global ecological issues. One on one interviews with four rural Georgia educators along with artifacts they provided were examined for themes related to two research questions. How do rural educators adapt their instructional strategies to …

Raise Your Digital Hand – Multilingual Learner Student And Teacher Perceptions Of Live Digital Feedback, Naveed Khan

Dissertations

Teachers of Multilingual Learners (MLs) must support students with varying English proficiency as they work toward meaningful academic progress. One promising support is live digital feedback, which can be multimodal—written, typed, drawn, or audiovisual—and serves as an added scaffold as MLs work within their zone of proximal development (ZPD). This qualitative case study examined ML student and teacher perceptions of live teacher and peer digital feedback in one-to-one (1:1) classrooms, grounded in sociocultural theory, effective feedback, and multimodality. The research questions focused on the types of feedback provided by teachers and peers and how ML students—both recipients and peer helpers—and …

Establishing Procedures For Primary Human Myoblast Cell Culture, Erica Duffy

Biomedical Engineering

Peripheral Artery Disease (PAD) affects an estimated 200 million people worldwide and is the leading cause of amputation in the United States. PAD is characterized by atherosclerotic narrowing of arteries, resulting in ischemia from reduced blood flow to distal tissues. Although surgical and percutaneous interventions can restore perfusion, fewer than 50% of patients experience sustained benefit, highlighting the need for alternative therapeutic strategies. One promising approach is promoting arteriogenesis, the growth and outward remodeling of pre-existing collateral vessels that improve blood flow around the occluded arteries. Arteriogenesis is regulated by regenerative, M2-polarized macrophages, making macrophage polarization a key therapeutic target. …

A Human Factors Analysis And Classification System (Hfacs) Approach To Compare Cardiovascular, Orthopedics, Trauma Care, And Neurosurgery, Asfandyar Khan

Doctoral Dissertations and Master's Theses

Background: Despite significant progress in patient safety, human error continues to occur at high rates in procedural settings. Traditional safety models tend to focus on retrospective incident analysis, often overlooking the systemic conditions that allow errors to occur. The Human Factors Analysis and Classification System (HFACS) offers a proactive lens to understand how and where errors emerge and is useful for identifying active and latent failures.

Objective: To evaluate the utility and reliability of the Human Factors Analysis and Classification System (HFACS) in categorizing and comparing the pattern of frequencies (events) in cardiovascular, orthopedic, trauma care, and neurosurgical procedure.

Design, …