Chemical Engineering Commons^™

Sustained Cell Differentiation Of 2d H9 Human Embryonic Stem Cells Into Mesenchymal Stem Cells, Hannah M. Christian

UCARE: Research Products

This experiment consisted of the controlled differentiation of H9 embryonic stem cells to mesenchymal stem cells. Though this experiment was repeated twice and improvement was seen in these repetitions, the cells were only able to be partially differentiated. However, the morphology of the differentiated cells is similar to those of healthy adult mesenchymal stem cells.

The progression of the differentiation can be seen in the microscope slide photos below. Throughout the differentiation, there occurred a decrease in cell survival and reduction of cell growth, but an increase in mesenchymal stem cell morphology. Throughout the last week of the differentiation, very …

Optimizing Chemical & Rheological Properties Of Rejuvenated Bitumen, Dominic Nguyen, Hamzeh Haghshenas Fatmehsari, Santosh Kommidi, Yong-Rak Kim

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Bitumen has long been a material used in the construction of roadways, yet new pavement only consists of low fractions of recycled materials due to poor compatibility of aged bitumen and new materials. Thus, rejuvenators, chemical additives, have been used in an attempt to re-balance the chemical composition and restore the physical properties of aged bitumen back to its virgin state. A fundamental understanding of how one particular rejuvenator, soybean oil, revitalizes bitumen was investigated using a multi-scale approach.

Fourier-transform infrared spectroscopy (FTIR) was used to determine the changes in chemical properties of pure and rejuvenated virgin and aged samples. …

Protein Synthesis Directly From Pcr: Progress And Applications Of Cell-Free Protein Synthesis With Linear Dna, Song-Min Schinn, Andrew Broadbent, William T. Bradley, Bradley Charles Bundy

Faculty Publications

A rapid, versatile method of protein expression and screening can greatly facilitate the future development of therapeutic biologics, proteomic drug targets and biocatalysts. An attractive candidate is cell-free protein synthesis (CFPS), a cell-lysate-based in vitro expression system, which can utilize linear DNA as expression templates, bypassing time-consuming cloning steps of plasmid-based methods. Traditionally, such linear DNA expression templates (LET) have been vulnerable to degradation by nucleases present in the cell lysate, leading to lower yields. This challenge has been significantly addressed in the recent past, propelling LET-based CFPS as a useful tool for studying, screening and engineering proteins in a …

Effect Of Packaging Material On Color Properties Of Catsup Tomato Sauce, Naser Kohan-Nia, Babak Pakbin, Razzagh Mahmoudi, Omid Fakhri

Journal of Applied Packaging Research

Catsup and tomato sauce products currently are one of the most popular and commercially available table sauces particularly consumed by young people and families around the world in recent decades. In this study colorimetric properties of catsup sauce samples including L*, a* and b* values in different packaging materials including PP, PE, PET, Glass and Sachet have been investigated during and after 180 days storage time at 22̊ C (environmental temperature). Hunter Lab system has been used to evaluate the color values of sauce samples. According to obtained results of experimental measurements and sensory evaluations, PP and PE packaging materials …

Immobilization Of Cellulase For Large Scale Reactors To Reduce Cellulosic Ethanol Cost, Dezhi Zhang

Doctoral Dissertations

Cellulosic ethanol is an alternative renewable energy source. Cellulase used in the production of cellulosic ethanol is very expensive. The difficulty in separating cellulase from the cellulose solution after the hydrolysis process limits the reusability of the cellulase, which highly precludes the scales of this application because of the high cost of the enzyme. Immobilization of cellulase provides a promising approach to allow the enzyme to be recycled, thus reducing the production cost. This research focused on immobilizing cellulase for reuse to reduce the cellulosic ethanol cost.

Four immobilization techniques were explored for the immobilization of cellulase on four different …

Manufacturing Polyacrylonitrile Nanowires And Nanofibers For Sensing And Energy Storage Applications, Juan Chen

Doctoral Dissertations

A novel flow guided assembly approach is presented to well align and accurately position nanowire arrays in pre-defined locations with high throughput and large scale manufacturing capability. In this approach, polyacrylonitrile (PAN)/N, N-dimethylformamide (DMF) solution is first filled in an array of microfluidic channels. Then a gas flow is introduced to blow out most solutions while pushing a little leftover against the channel wall to assemble into polymer nanowires. In this way, highly-ordered nanowires are conveniently aligned in the flow direction and patterned along both sides of the microchannels. In this study, we demonstrated this flow-guided assembly process by producing …

Thermal Properties, Crystallinity, And Oxygen Permeability Of Na-Montmorillonite Reinforced Plasticized Poly(Lactic Acid) Film, Kurniawan Yuniarto, Yohanes Aris Purwanto, Setyo Purwanto, Bruce A. Welt, Hadi Karia Purwadaria, Titi Candra Sunarti

Makara Journal of Technology

Introducing unmodified organically clay/Na-montmorillonite (Na-MMT) was applied into plasticized poly(lactic acid) PLA to produce film composites by direct casting. Film composite structure, the crystallinity degree and form, and thermal properties were carried out using X-ray diffraction and differential scanning calorimetry. The effect of Na- MMT to the tortuous path and the crystallinity degree in the plasticized film composites were calculated in oxygen barrier properties. Chromatogram film composites resulted in an intercalated structure that showed peak diffraction angle shift at about 0.2o. Then, a peak diffraction pattern was indicated in α-form crystal structure. Plasticized PLA has a crystallinity degree at 34%, …

Residual Strength Analysisof Asymmetrically Damaged Ship Hull Girderusing Beam Finite Element Method, Muhammad Zubair Muis Alie

Makara Journal of Technology

The objective of the present study is to analyze the residual strength of asymmetrically damaged ship hull girder under longitudinal bending. Beam Finite Element Method is used for the assessment of the residual strength of two single hull bulk carriers (Ship B1 and Ship B4) and a three-cargo-hold model of a single-side Panamax Bulk Carrier in hogging and sagging conditions. The Smith’s method is adopted and implemented into Beam Finite Element Method. An efficient solution procedure is applied; i.e. by assuming the cross section remains plane, the vertical bending moment is applied to the cross section and three-cargo-hold model. As …

Discrete Power Control In Heterogenous Networks, Yuanyuan Wang, Wibowo Hardjawana, Branka Vucetic

Makara Journal of Technology

In heterogeneous networks (HetNets) where femtocell base stations (FBSs) are deployed within the radio coverage of macrocell base stations (MBSs) to increase network capacity, co-channel interference limits overall system performance with universal frequency reuse. This paper investigates new distributed downlink discrete power control scheme for FBSs in HetNets with FBSs cooperation. The objective of the proposed power control scheme is to maximize the number of simultaneous FBSs transmissions in a single transmission wireless channel where each FBS is allowed to transmit only if the signal-to-interference-noise ratio (SINR) requirements for both FBSs and MBS users are satisfied. We apply a stochastic …

Multi-Project Scheduling Cost Optimization In A Machine Manufacturer Engineer-To-Order, Farizal Farizal, Amar Rachman, Tifani Tandean, Sumarsono Sudarto, Katsuhiko Takahashi

Makara Journal of Technology

This paper discusses the utilization of mixed integer linear programming (MILP) model to optimize cost for multiproject scheduling in a machine maker company. The objective is to minimize total project’s penalty cost and labor cost. The model formulated shows how to achieve the objective i.e. whether to use outsourcing or overtime to finish all projects. The model of multi-project scheduling was solved by Branch & Bound algorithm coded in Lingo 14.0 software. The case study shows that if a company wants to minimize lateness, it should use overtime instead of outsourcing, which minimize total lateness of projects by 144 days …

Analysis Of Arm Movement Prediction By Using The Electroencephalography Signal, Reza Darmakusuma, Ary Setijadi Prihatmanto, Adi Indrayanto, Tati Latifah Mengko, Lidwina Ayu Andarini, Achmad Furqon Idrus

Makara Journal of Technology

Various technological approaches have been developed in order to help those people who are unfortunate enough to be afflicted with different types of paralysis which limit them in performing their daily life activities independently. One of the proposed technologies is the Brain-Computer Interface (BCI). The BCI system uses electroencephalography (EEG) which is generated by the subject’s mental activity as input, and converts it into commands. Some previous experiments have shown the capability of the BCI system to predict the movement intention before the actual movement is onset. Thus research has predicted the movement by discriminating between data in the “rest” …

Single Cell Encapsulation Using A Microfluidic Droplet Generator, Seleipiri Charles

Honors Capstones

No abstract provided.

Core-Shell Nanoparticles For The Desulfurization Of Biogas, Joshua Saltz

Honors Capstones

No abstract provided.

Modeling The Effects Of Mass Transport On The Electrochemical Reduction Of Co2 At Cu2o Surfaces, Edward Alexander Thistlethwaite Iii

Honors Capstones

No abstract provided.

The Exacerbating Effect Of Coastal Erosion To Orphan Well Risk Analysis In Terrebonne Parish, Taylor Reeves

Honors Capstones

No abstract provided.

Modeling Of Reaction Kinetics And Mass Transfer Of Carbon Dioxide Electrochemical Reduction, Sneha Seetharama

Honors Capstones

No abstract provided.

Targeted Metabolomics On The Shikimate And Aromatic Amino Acid Biosynthetic Pathways, Robert E. Wheeler

Open Access Theses

The shikimate and aromatic amino acid biosynthesis pathways are some of the most studied biosynthetic pathways in nature due to their fundamental importance. Interest in this field stems from synthesis of the essential amino acids, tryptophan, tyrosine and phenylalanine. The pathways also are important as generating the precursors of thousands of secondary metabolites and lignin. Inhibiting the pathway has led to discovery of herbicides, pesticides as well as tuberculosis drugs. However there is currently a lack of a comprehensive targeted method for detecting and quantifying the majority of the intermediates in these pathways. Often multiple extraction and detection methods are …

Brain Tumor In A Dish: Glioma/Astrocyte Co-Cultures As A Model For In Vitro Studies, Erin Eickman, Christina Wilson, Srivatsan Kidambi

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This study seeks to engineer an in vitro co-culture model to elucidate the role of glioma-astrocyte interactions on molecular changes in the tumor microenvironment. The use of patterned co-cultures created with polyelectrolyte multilayers and micromolding in capillaries will allow tthe investigation of cell-cell communication. This study will lead to better understanding of the role of healthy cells in cancer progression and potential treatment options.

Investigation Of A Nanoparticle Delivery System For Micronutrients In Corn, Zully Perez Sierra, Zachary P. Stewart, Hendrik J. Viljoen

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Pheroid Nanoparticle Delivery System1 • Cost effective for plant applications • Safe – multi-lamellar lipid system derived mostly from soybeans • Versatile – payloads delivered range from micronutrients to antibiotics Micronutrient Deficiency • Caused by the immobility of micronutrients through the plant. • Leads to malnutrition children in poor countries2 • As fertilizers deliver nutrients, only small percentage obtained by plant. Purpose of Nanoparticle Delivery System • Increase plant micronutrient content and yield • Reduces the effects of run-of, overuse and pollution Other Nano-Fertilizer Trials • Desert plant results – in a foliar application versus irrigation application, the foliar nano-fertilizer …

Ionic Driven Embedment Of Hyaluronic Acid Coated Liposomes In Polyelectrolyte Multilayer Films For Local Therapeutic Delivery, Stephen Lawrence Hayward, David Francis, Matthew J. Sis, Srivatsan Kidambi

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The ability to control the spatial distribution and temporal release of a therapeutic remains a central challenge for biomedical research. Here, we report the development and optimization of a novel substrate mediated therapeutic delivery system comprising of hyaluronic acid covalently functionalized liposomes (HALNPs) embedded into polyelectrolyte multilayer (PEM) platform via ionic stabilization. The PEM platform was constructed from sequential deposition of Poly-LLysine (PLL) and Poly(Sodium styrene sulfonate) (SPS) “(PLL/SPS)4.5” followed by adsorption of anionic HALNPs. An adsorption affinity assay and saturation curve illustrated the preferential HALNP deposition density for precise therapeutic loading. (PLL/SPS)2.5 capping layer on top of the deposited …