Biochemical and Biomolecular Engineering Commons^™

Development, Validation And Implementation Of Multiple Radioactive Particle Tracking Technique, Mehul S. Vesvikar, Thaar M. Aljuwaya, Mahmoud M. Taha, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

Computer Automated Radioactive Particle Tracking (CARPT) technique has been successfully utilized to measure the velocity profiles and mixing parameters in different multiphase flow systems where a single radioactive tracer is used to track the tagged phase. However, many industrial processes use a wide range of particles with different physical properties where solid particles could vary in size, shape and density. For application in such systems, the capability of current single tracer CARPT can be advanced to track more than one particle simultaneously. Tracking multiple particles will thus enable to track the motion of particles of different size shape and density, …

Kinetic Assessment Of Light Hydrocarbons Separation Over Fe-Doped 13x Composite Sorbents Under Multicomponent Feed Conditions, Khaled Baamran, Jimmy D.L. Moreno, Ali A. Rownaghi, Fateme Rezaei

Chemical and Biochemical Engineering Faculty Research & Creative Works

Current sorbents investigated for light olefin/paraffin separation usually suffer from low selectivity. Besides, multicomponent analysis of this important separation is usually overlooked in the literature. To enhance the separation efficiency of zeolite 13X, we developed a series of Fe₂O₃/13X composite sorbents and assessed their separation performance using binary, ternary, and multicomponent gas mixtures of C₂H₄, C₂H₆, CH₄, and H₂. In these composites, nano- and micro Fe₂O₃ particles (NPs and MPs) with varied loadings were used, while Fe was also ion-exchanged into …

Hydrothermal Stability Of Na-Lta Shaped With Clay Binder, Jimmy D.L. Moreno, Daniele Da S. Pereira, Thalita M. Azevedo, Dárley C. De Melo, Debora A.S. Maia, Fateme Rezaei, Moisés Bastos-Neto, Diana C.S. De Azevedo

Chemical and Biochemical Engineering Faculty Research & Creative Works

Gas drying by adsorption on molecular sieves is one of the first unit operations in natural gas processing. Nevertheless, the thermal swings during regeneration combined with the presence of a complex gas mixture may lead to early adsorbent fouling. The shaping of adsorbent materials is crucial not only to reduce pressure drop in columns, but also for enhanced thermal and mechanical properties. In this work, synthesized sodium-based zeolite Linde Type A (Na-LTA) in powder was shaped by extrusion into cylindrical pellets using a clay binder followed by calcination at 673 K for 24 h. The produced 2-mm pellets had binder …

Insights To Surfactant Huff-Puff Design In Carbonate Reservoirs Based On Machine Learning Modeling, Ya Yao, Yue Qiu, Yue Cui, Mingzhen Wei, Baojun Bai

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Surfactants could react with adsorbates on carbonate rock surface to alter wettability from oil-wetness to water-wetness, which is effective to enhance oil recovery. Surfactant huff-puff treatment is mostly applied for this purpose and the resulting surfactant performance is the outcome of complex interfacial processes. Currently, the effect of important parameters on surfactant performance is not completely reported and the contribution of each parameter to surfactant performance is hard to be quantified. Traditional methods to optimize surfactant performance are time-consuming and show strong dependency on extensive experiments. in this paper, we address these problems from machine learning (ML) perspectives. Several ML …

Effect Of Eggshell Powder And Nano-Hydroxyapatite On The Surface Roughness And Microhardness Of Bleached Enamel, Shahinaz Hassan, Lamiaa Moharam

Chemical and Biochemical Engineering Faculty Research & Creative Works

Background: The aim of the study was to evaluate the remineralizing potential of prepared solutions of eggshell powder (ESP) and nano-hydroxyapatite (nHA) on the surface roughness and microhardness of bleached enamel. Materials and Methods: Fifty bovine anterior teeth were selected and cleaned then bleached using a chemically activated in-office bleaching agent then the teeth were randomly allocated into five groups (n = 10) according to the tested remineralizing agents (10% ESP solution, 10% nHA solution, and MI Paste Plus) into; control, bleached, bleached + ESP solution, bleached + nHA solution, and bleached + MI Paste Plus groups. Then, the teeth …

Investigation Of Mechanical Regulation On Stat3 Activity And Mmp Production, Jaxson R. Libby

Honors Theses and Capstones

Transcription factor, STAT3, is inappropriately expressed in cancer cells, and has contrasting activation in 2D versus 3D microenvironments. 2D plates are often used for drug screening and do not always recapitulate in vivo responses. To combat inaccurate 2D drug studies, a 3D hydrogel was created to support the growth of cancer cells into a tumor-like environment. The hydrogel consists of a biocompatible dextran homopolysaccharide, cell adhesion RGD sequences, and crosslinker MMP labile peptides. A pH dependent reaction couples the RGD sequences to dextran then the polymers are crosslinked into a gel. Crosslinking is accomplished using terminal cysteine peptide sequences, allowing …

Comparative Study Of Proppant Transport In The Vertical Fracture For High Viscosity Friction Reducers And Slickwater Fracturing With High-Tds Marcellus Shale Formation Water, Xiaojing Ge, Abdulmohsin Imqam, Baojun Bai

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

High viscosity friction reducers (HVFRs) have been gaining more attention and increasing in use as proppant carriers. Reusing of produced water has also been driven by both environmental and economic benefits. However, high total dissolved solids (TDS) of produced water and high reservoir temperature significantly decrease the viscoelastic properties of HVFRs even for the cationic ones, which further reduce their proppant transport capabilities. Increasing loadings of HVFRs also has very limited effects on improving their proppant transport performances under high-TDS and high-Temperature conditions. The ultimate objective of this experimental study is to investigate the injection rate effects on the proppant …

Exploring Ph Gradient Phenomena In Non-Linear Electrokinetic Microfluidic Devices, Azade Tahmasebi

Dissertations, Master's Theses and Master's Reports

Electrokinetic microfluidics is a versatile technology utilized within lab on a chip (LOC) devices for diagnostic and analytical applications; advantages include reduced resource demands, flexibility, and simplicity of use. Dielectrophoresis (DEP) is a precision nonlinear electrokinetic tool utilized within microfluidic microdevices to induce polarization and control bioparticle motions for applications that range from hemoglobin separations to cancer cell isolation and detection. Despite promising results, undesired side phenomena can occur in electrokinetic systems which impede reproducibility and accuracy. These unfavorable phenomena have not been comprehensively explored in the literature. Prior preliminary research suggests the fundamental phenomena originate from microelectrodes utilized in …

A Comprehensive Review Of The Influence Of Heat Exchange Tubes On Hydrodynamic, Heat, And Mass Transfer In Bubble And Slurry Bubble Columns, Dalia S. Makki, Hasan Sh Majdi, Amer A. Abdulrahman, Abbas J. Sultan, Zahraa W. Hasan, Laith S. Sabri, Bashar J. Kadhim, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

Bubble and slurry bubble column reactors (BCRs/SBCRs) are used for various chemical, biochemical, and petrochemical applications. They have several operational and maintenance advantages, including excellent heat and mass transfer rates, simplicity, and low operating and maintenance cost. Typically, a catalyst is present in addition to biochemical processes where microorganisms are used to produce industrially valuable bio-products. Since most applications involve complicated gas-liquid, gas-liquid-solid, and exothermic processes, the BCR/SBCR must be equipped with heat-exchanging tubes to dissipate heat and control the reactor's overall performance. In this review, past and very recent experimental and numerical investigations on such systems are critically discussed. …

Effect Of Alkali Metal Functionalization On Hydrogen Storage Performance Of Highly Porous Activated Carbons, Ruthradharshini Murugavel, Ali A. Rownaghi, Fateme Rezaei

Chemical and Biochemical Engineering Faculty Research & Creative Works

With increasing energy demand and fast-paced decarbonization methods adopted to curb the adverse effects of greenhouse gas emissions, hydrogen (H2) is considered a sustainable, clean, and potential renewable energy carrier for the future. To substitute the energy needs required for the physical methods of H2 storage, adsorbed gas storage (AGS) is the next viable option that provides the opportunity to adsorb and desorb gases under mild conditions. In this work, we studied the effect of light alkali metal functionalization comprising Li and K on the H2 storage performance of porous graphitic carbon adsorbents. The effect of metal doping on the …

Impact Of Composition And Salinity On Swelling And Gel Strength Of Poly (Acrylamide-Co-Acrylic Acid) Preformed Particle Gel, Ahmed Ben Ali, Reem Elaf, Mohammed Saad, Ibnelwaleed A. Hussein, Baojun Bai

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

The effects of various material compositions and reservoir environments on the ultimate strength and swelling kinetics of a commercial preformed particle gel (PPG) have been investigated. This study used different ratios of acrylamide and acrylic acid copolymers with a specific crosslinker concentration. Results have indicated that increasing the acrylic acid proportion enhances the PPGs' ability to swell but weakens their network structure. In contrast, increasing the crosslinker content decreases the swelling ratio and increases the gel strength. The highest equilibrium swelling capacity among the six preformed particle gel samples was obtained for PPG2, which has the highest acrylic acid amount …

The Success Story Of First Ever Polymer Flood Field Pilot To Enhance The Recovery Of Heavy Oils On Alaska's North Slope, Abhijit Dandekar, Baojun Bai, John Barnes, Dave Cercone, Reid Edwards, Samson Ning, Randy Seright, Brent Sheets, Dongmei Wang, Yin Zhang

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

The primary goal of the first ever polymer flood field pilot at Milne Point is to validate the use of polymers for heavy oil Enhanced Oil Recovery (EOR) on Alaska North Slope (ANS). The specific objectives are systematic evaluation of advanced technology that integrates polymer flooding, low salinity water flooding, horizontal wells, and numerical simulation based on polymer flood performance data. Accordingly, under the co-sponsorship of the US Department of Energy and Hilcorp Alaska LLC the first ever polymer field pilot commenced on August 28, 2018 in the Schrader Bluff heavy oil reservoir at the Milne Point Unit (MPU) on …

Transport And Plugging Performance Evaluation Of A Novel Re-Crosslinkable Microgel Used For Conformance Control In Mature Oilfields With Super-Permeable Channels, Adel Alotibi, T. Song, Baojun Bai, Thomas P. Schuman

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Preformed particle gels (PPG) have been widely applied in oilfields to control excessive water production. However, PPG has limited success in treating opening features because the particles can be flushed readily during post-water flooding. We have developed a novel micro-sized Re-crosslinkable PPG (micro-RPPG) to solve the problem. The microgel can re-crosslink to form a bulk gel, avoiding being washed out easily. This paper evaluates the novel microgels' transport and plugging performance through super-permeable channels. Micro-RPPG was synthesized and evaluated for this study. Its storage moduli after fully swelling are approximately 82 Pa. The microgel characterization, self-healing process, transportation behavior, and …

Cross-Seeding Of Protegin-1 And Amyloid Beta To Inhibit Amyloid Beta Aggregation, Sarah Robinson

Williams Honors College, Honors Research Projects

Amyloid beta (Aβ) is considered a major component in the progression of degenerative disease like Alzheimer’s disease. In an attempt to cure Alzheimer’s before it has presented, this project proposes a study on the effects of protegrin-1 (PG-1) on the aggregation of Aβ. By cross-seeding to form a PG-1- Aβ heterocomplex, it is believed that aggregation of Aβ on the human brain can be inhibited, thus helping to stop the neurodegeneration caused by Alzheimer’s disease. To complete this project, an initial literature review of previous work done by the graduate student leading the project will be completed. After this, experiments …

Microscopic And Laboratory Scale Characterization Methods To Evaluate Biomass Deconstruction, Meenaa Chandrasekar

Dissertations, Master's Theses and Master's Reports

Renewable fuels from lignocellulosic biomass are an appealing option because they can seamlessly integrate into the existing fuel distribution infrastructure. Lignocellulosic biomass constitutes nonedible plant material obtained from plant cell walls. The natural recalcitrance of lignocellulosic biomass poses a challenge in accessing the cell wall carbohydrates during biochemical conversion. Despite various approaches, enzymatic hydrolysis of lignocellulosic biomass remains economically impractical due to incomplete knowledge about biomass recalcitrance and the influence of environmental factors on biomass quality.

The first goal of this dissertation was to construct a microfluidic imaging reactor to better understand the tissue-specific deconstruction of plant materials. Confocal laser …

Increased Liver Stiffness Promotes Hepatitis B Progression By Impairing Innate Immunity In Ccl4-Induced Fibrotic Hbv+ Transgenic Mice, Grace Bybee, Youra Moeun, Weimin Wang, Kusum K. Kharbanda, Larisa Y. Poluektova, Srivatsan Kidambi, Natalia A. Osna, Murali Ganesan

Department of Chemical and Biomolecular Engineering: Faculty Publications

Background: Hepatitis B virus (HBV) infection develops as an acute or chronic liver disease, which progresses from steatosis, hepatitis, and fibrosis to end-stage liver diseases such as cirrhosis and hepatocellular carcinoma (HCC). An increased stromal stiffness accompanies fibrosis in chronic liver diseases and is considered a strong predictor for disease progression. The goal of this study was to establish the mechanisms by which enhanced liver stiffness regulates HBV infectivity in the fibrotic liver tissue. Methods: For in vitro studies, HBV-transfected HepG2.2.15 cells were cultured on polydimethylsiloxane gels coated by polyelectrolyte multilayer films of 2 kPa (soft) or 24 kPa (stiff) …

Mesenchymal Stromal Cells And Alpha-1 Antitrypsin Have A Strong Synergy In Modulating Inflammation And Its Resolution, Li Han, Xinran Wu, Ou Wang, Xiao Luan, William Velander, Michael Aynardi, E. Scott Halstead, Anthony S. Bonavia, Rong Jin, Guohong Li, Yulong Li, Yong Wang, Cheng Dong, Yuguo Lei

Department of Chemical and Biomolecular Engineering: Faculty Publications

Rationale: Trauma, surgery, and infection can cause severe inflammation. Both dysregulated inflammation intensity and duration can lead to significant tissue injuries, organ dysfunction, mortality, and morbidity. Anti-inflammatory drugs such as steroids and immunosuppressants can dampen inflammation intensity, but they derail inflammation resolution, compromise normal immunity, and have significant adverse effects. The natural inflammation regulator mesenchymal stromal cells (MSCs) have high therapeutic potential because of their unique capabilities to mitigate inflammation intensity, enhance normal immunity, and accelerate inflammation resolution and tissue healing. Furthermore, clinical studies have shown that MSCs are safe and effective. However, they are not potent enough, alone, to …

Prediction Of Single Proppant Terminal Settling Velocity In High Viscosity Friction Reducers By Using Artificial Neural Networks And Xgboost, Xiaojing Ge, Rong Lu, Ghith Biheri, Abdulmohsin Imqam, Baojun Bai

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

High viscosity friction reducers (HVFRs) have been recently gaining more attention and increasing in use, not only as friction-reducing agents but also as proppant carriers. The settling velocity of the proppant is one of the key outputs to describe their proppant transport capability. However, it is influenced by many factors such as fluid properties, proppant properties, and fracture properties. Many empirical/physics-based models and correlations to predict particle settling velocity have been developed. However, they are usually based on certain assumptions and have applicable limits. In contrast, machine learning models can be considered as a black box. The objective of this …

Leveraging Cell-Substrate Adhesion And Cell Migratory Properties In Skeletal Muscle Constructs And Cancer Metastasis Assays, Lauren E. Mehanna

Theses and Dissertations--Chemical and Materials Engineering

Volumetric muscle loss (VML) remains one of the few skeletal muscle injuries without a reliable and repeatable treatment. In large volume muscle injuries, muscle fibers as well as the surrounding connective tissue are damaged, preventing therapeutic muscle stem cells, called myogenic progenitor cells (MPCs), from reaching the injury site and initiating repair. There is a clinical need to rapidly fabricate in vitro muscle tissue constructs that mimic the native tissue organization, with aligned myotubes, for insertion and integration at the patient’s injury site. In this dissertation, we utilize the MPC’s natural propensity to close gaps across an injury site to …

Chemical Decomposition Of Flexible Polyurethane Foam To Generate A Media For Microbial Upcycling, Kaushik Baruah

Dissertations, Master's Theses and Master's Reports

Polyurethane waste is becoming a global concern as a large amount is being disposed of in landfills every year, and only a fraction is being recycled. Several polyurethane recycling techniques exist, of which ammonolysis and base-catalyzed hydrolysis is the least explored. Flexible polyurethane foam (FPUF) decomposition can generate amines that can act as a carbon source for the growth of microbial consortia. This study aims to generate a novel media capable of microbial upcycling via ammonolysis and base-catalyzed hydrolysis of flexible polyurethane foams (FPUFs) using ammonium hydroxide and subsequently determine the reaction conditions for maximum solubilization of polyurethane foam in …