Multimode Analysis Of Nanoscale Biomolecular Interactions,
2015
Florida International University
Multimode Analysis Of Nanoscale Biomolecular Interactions, Purushottam Babu Tiwari
FIU Electronic Theses and Dissertations
Biomolecular interactions, including protein-protein, protein-DNA, and protein-ligand interactions, are of special importance in all biological systems. These interactions may occer during the loading of biomolecules to interfaces, the translocation of biomolecules through transmembrane protein pores, and the movement of biomolecules in a crowded intracellular environment. The molecular interaction of a protein with its binding partners is crucial in fundamental biological processes such as electron transfer, intracellular signal transmission and regulation, neuroprotective mechanisms, and regulation of DNA topology. In this dissertation, a customized surface plasmon resonance (SPR) has been optimized and new theoretical and label free experimental methods with related analytical …
Sulfidic Spring In The Gypsum Karst System Of Monte Conca (Italy): Chemistry And Microbiological Evidences,
2015
Centro Speleologico Etneo, Catania, Italy
Sulfidic Spring In The Gypsum Karst System Of Monte Conca (Italy): Chemistry And Microbiological Evidences, Marianna Messina, Tiziana Grech, Fiorenzo Fiorenza, Alessandro Marlettta, Pietro Valenti, Salvatore Petralia
International Journal of Speleology
Monte Conca Cave is a karst system placed in Messinian evaporites, consisting of an active cave and a resurgence located on the massif of Monte Conca, Campofranco within the "Riserva Naturale Integrale di Monte Conca". A sulfidic spring is located in the terminal gallery of the cave. To characterize the physical and chemical parameters of the Monte Conca cave and of the sulfidic spring, air temperature, relative humidity, water pH, and concentrations of dissolved sulfides, nitrates and sulfates were monitored. The high sulfide consumption rate in the sulfidic spring, evaluated by a kinetic study, suggests that biotic consumption is dominant. …
A Study Of Volatile Organic Compounds From Transgenic Arabidopsis Thaliana And Solanum Lycopersicum Plants And Analytical Characterization Of Pyrolysis Bio-Oil,
2015
The University of Western Ontario
A Study Of Volatile Organic Compounds From Transgenic Arabidopsis Thaliana And Solanum Lycopersicum Plants And Analytical Characterization Of Pyrolysis Bio-Oil, Luis A. Caceres
Electronic Thesis and Dissertation Repository
A dynamic headspace method was developed for the non-targeted analysis of volatile organic compounds (VOC) in Arabidopsis and Micro-Tom plants. The method allowed to determine differences from wild type (WT) and transformed plants overexpressing the carotene cleavage dioxygenase 1 (CCD1) genes. In the Arabidopsis study it was revealed the presence of unsaturated hydrocarbons, aromatic derivatives, alcohols, and terpenes. Among extracted volatiles, the apocarotenoid compound β-ionone was also detected, four-fold higher in the transgenic plants than the WT. There were not significant differences in the VOCs between the HS of Micro-Tom tomato plants overexpressing the CCD1 genes and WT. …
Science Classics,
2015
University of Dayton
Science Classics, Mark Masthay
Mark Masthay
An essay on the impact of the works in the Imprints and Impressions: Milestones in Human Progress, an exhibition of rare books from the collection of Stuart Rose. Exhibition was held Sept. 29-Nov. 9, 2014, at the University of Dayton.
Synthetic Strategies For Tailoring The Physicochemical And Magnetic Properties Of Hydrophobic Magnetic Ionic Liquids,
2015
University of Toledo
Synthetic Strategies For Tailoring The Physicochemical And Magnetic Properties Of Hydrophobic Magnetic Ionic Liquids, Omprakash Nacham, Kevin D. Clark, Honglian Yu, Jared L. Anderson
Jared L. Anderson
Magnetic ionic liquids (MILs) are a subclass of ionic liquids (ILs) containing paramagnetic components and are readily manipulated by an external magnetic field. Due to their hydrophilic nature, very few applications of MILs in aqueous systems have been reported. In this study, three general classes of hydrophobic MILs including monocationic, symmetrical/unsymmetrical dicationic, and symmetrical/unsymmetrical tricationic MILs were synthesized and characterized. By tuning the structure of the MIL, various physicochemical properties including water solubility, magnetic susceptibility, and melting point were regulated. MILs synthesized with the benzimidazolium cation were shown to exhibit lower water solubility (0.1% (w/v)) when compared to those containing …
Infrared Spectra Of Substituted 1,10-Phenanthrolines ,
2015
Iowa State University
Infrared Spectra Of Substituted 1,10-Phenanthrolines , Robert Carl Smith
Dr Robert J Smith
No abstract provided.
Extraction Of Dna By Magnetic Ionic Liquids: Tunable Solvents For Rapid And Selective Dna Analysis,
2015
University of Toledo
Extraction Of Dna By Magnetic Ionic Liquids: Tunable Solvents For Rapid And Selective Dna Analysis, Kevin D. Clark, Omprakash Nacham, Honglian Yu, Tianhao Li, Melissa M. Yamsek, Donald R. Ronning, Jared L. Anderson
Jared L. Anderson
DNA extraction represents a significant bottleneck in nucleic acid analysis. In this study, hydrophobic magnetic ionic liquids (MILs) were synthesized and employed as solvents for the rapid and efficient extraction of DNA from aqueous solution. The DNA-enriched microdroplets were manipulated by application of a magnetic field. The three MILs examined in this study exhibited unique DNA extraction capabilities when applied toward a variety of DNA samples and matrices. High extraction efficiencies were obtained for smaller single-stranded and double-stranded DNA using the benzyltrioctylammonium bromotrichloroferrate(III) ([(C8)3BnN+][FeCl3Br–]) MIL, while the dicationic 1,12-di(3-hexadecylbenzimidazolium)dodecane bis[(trifluoromethyl)sulfonyl]imide bromotrichloroferrate(III) ([(C16BnIM)2C122+][NTf2–, FeCl3Br–]) MIL produced higher extraction efficiencies for larger …
Chloroamino Acids As A Chemical Explanation For Viking Labeled Release Soil Activity On Mars,
2015
California Polytechnic State University, San Luis Obispo
Chloroamino Acids As A Chemical Explanation For Viking Labeled Release Soil Activity On Mars, John C. Hironimus
STAR Program Research Presentations
The Viking missions to Mars, which took place in the 1970’s, performed several experiments on martian soil in an attempt to discern if there was microbial life present. In one set of experiments, a nutrient solution containing amino acids and organic substrates tagged with carbon-14 was injected into sample cells containing martian soil, and the evolution of labeled CO2 was monitored. The evolution of labeled CO2 indicated that there was a process occurring that broke down the nutrients in solution and released CO2 as a byproduct. Cellular metabolism releases CO2, so this result supported the idea of microbial life on …
Unraveling The Mechanism Of Selective Ion Transport In
Hydrophobic Subnanometer Channels,
2015
Chinese Academy of Sciences
Unraveling The Mechanism Of Selective Ion Transport In Hydrophobic Subnanometer Channels, Hui Li, Joseph S. Francisco, Xiao Cheng Zeng
Xiao Cheng Zeng Publications
Recently reported synthetic organic nanopore (SONP) can mimic a key feature of natural ion channels, i.e., selective ion transport. However, the physical mechanism underlying the K+/Na+ selectivity for the SONPs is dramatically different from that of natural ion channels. To achieve a better understanding of the selective ion transport in hydrophobic subnanometer channels in general and SONPs in particular, we perform a series of ab initio molecular dynamics simulations to investigate the diffusivity of aqua Na+ and K+ ions in two prototype hydrophobic nanochannels: (i) an SONP with radius of 3.2 Å, and (ii) single-walled …
Research-Based Implementation Of Peer Instruction:
A Literature Review,
2015
University of Nebraska-Lincoln
Research-Based Implementation Of Peer Instruction: A Literature Review, Trisha Vickrey, Kaitlyn Rosploch, Reihaneh Rahmanian, Matthew Pilarz, Marilyne Stains
Chemistry Department: Faculty Publications
Current instructional reforms in undergraduate science, technology, engineering, and mathematics (STEM) courses have focused on enhancing adoption of evidence-based instructional practices among STEM faculty members. These practices have been empirically demonstrated to enhance student learning and attitudes. However, research indicates that instructors often adapt rather than adopt practices, unknowingly compromising their effectiveness. Thus, there is a need to raise awareness of the research-based implementation of these practices, develop fidelity of implementation protocols to understand adaptations being made, and ultimately characterize the true impact of reform efforts based on these practices. Peer instruction (PI) is an example of an evidence-based instructional …
New Insight Into The Helium-Induced Damage In Max Phase Ti3Alc2
By First-Principles Studies,
2015
Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang
New Insight Into The Helium-Induced Damage In Max Phase Ti3Alc2 By First-Principles Studies, Yiguo Xu, Xiaojing Bai, Xianhu Zha, Qing Huang, Jian He, Kan Luo, Yuhong Zhou, Timothy C. Germann, Joseph S. Francisco, Shiyu Du
Chemistry Department: Faculty Publications
In the present work, the behavior of He in the MAX phase Ti3AlC2 material is investigated using first-principle methods. It is found that, according to the predicted formation energies, a single He atom favors residing near the Al plane in Ti3AlC2. The results also show that Al vacancies are better able to trap He atoms than either Ti or C vacancies. The formation energies for the secondary vacancy defects near an Al vacancy or a C vacancy are strongly influenced by He impurity content. According to the present results, the existence of trapped He atoms in primary Al vacancy can …
Communication: Spectroscopic Consequences Of Proton Delocalization
In Ochco+,
2015
Georgia Southern University
Communication: Spectroscopic Consequences Of Proton Delocalization In Ochco+, Ryan C. Fortenberry, Qi Yu, John S. Mancini, Joel M. Bowman, Timothy J. Lee, T. Daniel Crawford, William F. Klemperer, Joseph S. Francisco
Chemistry Department: Faculty Publications
Even though quartic force fields (QFFs) and highly accurate coupled cluster computations describe the OCHCO+ cation at equilibrium as a complex between carbon monoxide and the formyl cation, two notable and typical interstellar and atmospheric molecules, the prediction from the present study is that the equilibrium C∞v structure is less relevant to observables than the saddle-point D∞h structure. This is the conclusion from diffusion Monte Carlo and vibrational self-consistent field/virtual state configuration interaction calculations utilizing a semi-global potential energy surface. These calculations demonstrate that the proton “rattle” motion (ν6) has centrosymmetric delocalization of the …
Structural And Optical Properties Of Cobalt Slanted Nanopillars Conformally
Coated With Few-Layer Graphene,
2015
University of Nebraska—Lincoln
Structural And Optical Properties Of Cobalt Slanted Nanopillars Conformally Coated With Few-Layer Graphene, Peter M. Wilson, Alexey Lipatov, Daniel Schmidt, Eva Schubert, Mathias Schubert, Alexander Sinitskii, Tino Hofmann
Chemistry Department: Faculty Publications
Optical characterization of anisotropic multicomponent nanostructures is generally not a trivial task, since the relation between a material’s structural properties and its permittivity tensor is nonlinear. In this regard, an array of slanted cobalt nanopillars that are conformally coated with few-layer graphene is a particularly challenging object for optical characterization, as it has a complex anisotropic geometry and comprises several materials with different topologies and filling fractions. Normally, a detailed characterization of such complex nanostructures would require a combination of several microscopic and spectroscopic techniques. In this letter, we demonstrate that the important structural parameters of these graphene-coated sculptured thin …
A Comparison Of Energy-Resolved Vibrational
Activation/Dissociation Characteristics Of Protonated
And Sodiated High Mannose N-Glycopeptides,
2015
University of Nebraska-Lincoln
A Comparison Of Energy-Resolved Vibrational Activation/Dissociation Characteristics Of Protonated And Sodiated High Mannose N-Glycopeptides, Forouzan Aboufazeli, Venkata Kolli, Eric D. Dodds
Chemistry Department: Faculty Publications
Fragmentation of glycopeptides in tandem mass spectrometry (MS/MS) plays a pivotal role in site-specific protein glycosylation profiling by allowing specific oligosaccharide compositions and connectivities to be associated with specific loci on the corresponding protein. Although MS/MS analysis of glycopeptides has been successfully performed using a number of distinction dissociation methods, relatively little is known regarding the fragmentation characteristics of glycopeptide ions with various charge carriers. In this study, energy-resolved vibrational activation/ dissociation was examined via collision-induced dissociation for a group of related high mannose tryptic glycopeptides as their doubly protonated, doubly sodiated, and hybrid protonated sodium adduct ions. The doubly …
The Importance Of Context: An Exploration Of Factors Influencing The Adoption Of Student-Centered Teaching Among Chemistry, Biology, And Physics Faculty,
2015
Oregon Institute of Technology
The Importance Of Context: An Exploration Of Factors Influencing The Adoption Of Student-Centered Teaching Among Chemistry, Biology, And Physics Faculty, Travis J. Lund, Marilyne Stains
Chemistry Department: Faculty Publications
Background: Research at the secondary and postsecondary levels has clearly demonstrated the critical role that individual and contextual characteristics play in instructors’ decision to adopt educational innovations. Although recent research has shed light on factors influencing the teaching practices of science, technology, engineering, and mathematics (STEM) faculty, it is still not well understood how unique departmental environments impact faculty adoption of evidence-based instructional practices (EBIPs) within the context of a single institution. In this study, we sought to characterize the communication channels utilized by STEM faculty, as well as the contextual and individual factors that influence the teaching practices …
Design And Development Of Bodipy-Based Fluorescent Probes For Sensing And Imaging Of Cyanide, Zn (Ii) Ions, Lysosomal Ph And Cancer Cells,
2015
Michigan Technological University
Design And Development Of Bodipy-Based Fluorescent Probes For Sensing And Imaging Of Cyanide, Zn (Ii) Ions, Lysosomal Ph And Cancer Cells, Jingtuo Zhang
Dissertations, Master's Theses and Master's Reports - Open
BODIPY (4,4-Difluoro-3a,4a-diaza-s-indacene) dyes have gained lots of attention in application of fluorescence sensing and imaging in recent years because they possess many distinctive and desirable properties such as high extinction coefficient, narrow absorption and emission bands, high quantum yield and low photobleaching effect. However, most of BODIPY-based fluorescent probes have very poor solubilities in aqueous solution, emit less than 650 nm fluorescence that can cause cell and tissue photodamages compared with bio-desirable near infrared (650-900 nm) light. These undesirable properties extremely limit the applications of BODIPY-based fluorescent probes in sensing and imaging applications. In order to overcome these drawbacks, we …
Hfq Regulates Antibacterial Antibiotic Biosynthesis And Extracellular Lytic-Enzyme Production In Lysobacter Enzymogenes Oh11,
2015
Nanjing Agricultural University
Hfq Regulates Antibacterial Antibiotic Biosynthesis And Extracellular Lytic-Enzyme Production In Lysobacter Enzymogenes Oh11, Gaoge Xu, Yuxin Zhao, Liangcheng Du, Guoliang Qian, Fengquan Liu
Chemistry Department: Faculty Publications
Lysobacter enzymogenes is an important biocontrol agent with the ability to produce a variety of lytic enzymes and novel antibiotics. Little is known about their regulatory mechanisms. Understanding these will be helpful for improving biocontrol of crop diseases and potential medical application. In the present study, we generated an hfq (encoding a putative ribonucleic acid chaperone) deletion mutant, and then utilized a new genomic marker-free method to construct an hfq-complemented strain. We showed for the first time that Hfq played a pleiotropic role in regulating the antibacterial antibiotic bio- synthesis and extracellular lytic enzyme activity in L. enzymogenes. Mutation of …
Energetics, Structure, And Rovibrational Spectroscopic Properties
Of The Sulfurous Anions Sno− And Osn−,
2015
Georgia Southern University
Energetics, Structure, And Rovibrational Spectroscopic Properties Of The Sulfurous Anions Sno− And Osn−, Ryan C. Fortenberry, Joseph S. Francisco
Chemistry Department: Faculty Publications
The SNO− and OSN− anions are shown in this work to be very stable negatively charged species in line with other recent work [T. Trabelsi et al., J. Chem. Phys. 143, 164301 (2015)]. Utilizing established quartic force field techniques, the structural and rovibrational data for these anions are produced. The SNO− anion is less linear and has weaker bonds than the corresponding neutral radical giving much smaller rotational constants.OSN− is largely unchanged in these regards with inclusion of the additional electron.The S–N bond is actually stronger,and the rotational constants of OSN− versus OSN are …
Biotechnological Production
Of 1,2,4-Butanetriol: An Efficient
Process To Synthesize Energetic
Material Precursor From Renewable
Biomass,
2015
Chinese Academy of Sciences
Biotechnological Production Of 1,2,4-Butanetriol: An Efficient Process To Synthesize Energetic Material Precursor From Renewable Biomass, Yujin Cao, Wei Niu, Jiantao Guo, Mo Xian, Huizhou Liu
Chemistry Department: Faculty Publications
1,2,4-Butanetriol (BT) is a valuable chemical with extensive applications in many different fields. The traditional chemical routes to synthesize BT suffer from many drawbacks, e.g., harsh reaction conditions, multiple steps and poor selectivity, limiting its industrial production. In this study, an engineered Escherichia coli strain was constructed to produce BT from xylose, which is a major component of the lignocellulosic biomass. Through the coexpression of a xylose dehydrogenase (CCxylB) and a xylonolactonase (xylC) from Caulobacter crescentus, native E. coli xylonate dehydratase (yjhG), a 2-keto acid decarboxylase from Pseudomonas putida (mdlC) and …
Recyclable Magnetite Nanoparticle Catalyst For One-Pot
Conversion Of Cellobiose To 5-Hydroxymethylfurfural In Water,
2015
University of Nebraska - Lincoln
Recyclable Magnetite Nanoparticle Catalyst For One-Pot Conversion Of Cellobiose To 5-Hydroxymethylfurfural In Water, Anuja Bhalkikar, Zane C. Gernhart, Chin Li Cheung
Chemistry Department: Faculty Publications
Environmentally benign and easily recoverable magnetite nanoparticles (Fe3O4 NPs) were demonstrated to catalyze the one-pot conversion of cellobiose, a glucose disaccharide, to 5-hydroxymethylfurfural (5-HMF).Theconversion was achieved in water under hydrothermal conditions.The catalytic activity of Fe3O4 NPs surpassed those of iron (II) and iron (III) chlorides in this reaction. Optimized cellobiose conversion reactions catalyzed with Fe3O4 NPs gave the highest 5-HMF yields of 23.4 ± 0.6% at 160∘C for 24 hours. After three reuses, the Fe3O4 NP catalyst retained its catalytic activity with similar 5-HMF yields, demonstrating …
