Periodontics and Periodontology Commons^™

Accuracy Of Volumetric Analysis Software Packages In Assessment Of Tooth Volume Using Cbct, Saylee Nimbalkar

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to evaluate the accuracy of volumetric analysis software packages in assessment of tooth volume using cone beam computed tomography and compare them to the volume obtained using an optical digital scanner. Twenty-four single rooted teeth indicated for extraction were collected. Cone beam computed tomography scans of these teeth resting in a custom scan jig were taken. Three dimensional digital models were obtained from the DICOM image cone beam computed tomography files using segmentation software packages (Anatomage, Mimics, and Amira) and volumes were calculated for each of the segmented teeth. The teeth were then scanned …

Influence Of Low Temperature Degradation On Microstructural Integrity Of Zirconia Dental Implants, Mona Monzavi Rahimi

Loma Linda University Electronic Theses, Dissertations & Projects

Zirconia dental implants have become more popular in the field of implant dentistry. However, concerns have been raised regarding low temperature degradation and its influence on phase stability and micro-structural integrity in terms of increased micro-crack formation. In the case of dental implants, this could possibly result in delamination and interference with bone to implant contact and eventually lead to loss of integration and failure. Nevertheless, no study has reported the effects on commercially available Zirconia dental implants. The primary aims of this study was to determine if there is a correlation between ageing duration and 1) depth of tetragonal-monoclinic …

Filifactor Alocis, A Newly Appreciated Oral Pathogen, Fails To Induce The Respiratory Burst Response Of Human Neutrophils., Jacob S. Edmisson

College of Arts & Sciences Senior Honors Theses

Almost 50% of adult Americans suffer from periodontitis which is a bacterially induced inflammation of the tissue that surround and support the tooth. The accumulation of neutrophils, a critical cell component of the innate immune system, in the gingival crevice contributes to tissue damage. Filifactor alocis is a newly appreciated pathogen present in oral biofilms at periodontal disease sites. Studying the interactions between neutrophils and F. alocis will provide valuable information for delineating the role of this bacterium in periodontal disease and enhance our understanding of bacterial strategies to evade leukocytes’ antimicrobial mechanisms. The hypothesis that F. alocis modulates human …

Tobacco-Gene Activity Profile In Porphyromonas Gingivalis, Filifactor Alocis And Treponema Denticola., Neelima Chowdary Cherukumalli

Electronic Theses and Dissertations

Smoking is an established risk factor for periodontitis. Prior studies have shown that cigarette smoke extract (CSE) can induce profound phenotypic changes in Porphyromonas gingivalis and alters the virulence of this important periodontal pathogen. We hypothesized that CSE might also alter gene expression in established periodontal pathogens, Porphyromonas gingivalis and Treponema denticola, as well as in the emerging pathogen, Filifactor alocis. Oral bacteria were grown in CSE-conditioned medium (1000 ng/ml nicotine equivalents) or in unconditioned control medium. Total RNA was extracted and CSE-regulated genes were identified by comparison of the mRNA profiles of CSE with control cultures using RNA-Seq …

Porphyromonas Gingivalis Gingipains Induce A Pro-Inflammatory Extracellular Microenvironment : The Role Of Par-2 And Fibronectin., Jeffrey S. Marschall

Electronic Theses and Dissertations

Periodontitis is a chronic inflammatory disease that is characterized by severe tissue destruction of the gingiva and other tooth supporting structures; if left untreated, tooth loss and disintegration of the alveolar bone occurs. This chronic inflammatory state has been linked to other systemic diseases such as cardiovascular disease, diabetes, rheumatoid arthritis, and Alzheimer’s disease. Porphyromonas gingivalis is the major pathogenic microbe in periodontitis. The main virulence factors of P. gingivalis are the Arg-aa and Lys-aa gingipains, which are proteolytic enzymes implicated in a plethora of activities that allow P. gingivalis to subvert the human immune system in the oral cavity …

Finasteride Adverse Effects And Post-Finasteride Syndrome; Implications For Dentists, Stana Paunica, Marina Giurgiu, Andrei Vasilache, Ioana Paunica, Ion Motofei, Adriana Vasilache, Horia Traian Dumitriu, Anca Silvia Dumitriu

Journal of Mind and Medical Sciences

Finasteride is a 5α-reductase inhibitor widely used in present in the therapeutic approach of androgenic alopecia. Adverse effects consist in variable sign and symptoms, the most common being represented by mental troubles (reduced feeling of life pleasure or emotions, depression), physical impairments (loss of muscle tone and/or mass) and sexual complains (loss of libido and sexual potency). An increasing number of studies identify and describe even a post-finasteride syndrome (persistent adverse affects three months or more after finasteride cessation) or new adverse effects including but not limited at the skin level or oral cavity (marginal periodontium).

We intend to present …

Trabecular Bone Microarchitecture In Association With Systemic And Local Factors As Potential Predictors Of Implant Failure, Diego A. Camacho Dmd

Theses and Dissertations

Clinicians have described the success rates of dental implants. The use of implants is projected to increase in the future. With a 5-10% failure rate, it is unclear the exact factors that are associated with implant failures. To improve upon these success rates, it is critical to understand parameters associated with implant failure including: periodontitis, peri-implantitis, systemic diseases, site preparation, dental history of the implant site, bone quality, materials, occlusion, and treatment timelines. While bone quality is associated with failure, objective measures to assess bone quality and characteristics are scarce. Therefore, the aim of this study is to determine whether …

Treatment Of A Periodontic-Endodontic Lesion In A Patient With Aggressive Periodontitis, Mina D. Fahmy, Paul G. Luepke, Mohammed Ibrahim, Arndt Guentsch

School of Dentistry Faculty Research and Publications

Case Description: This case report describes the successful management of a left mandibular first molar with a combined periodontic-endodontic lesion in a 35-year-old Caucasian woman with aggressive periodontitis using a concerted approach including endodontic treatment, periodontal therapy, and a periodontal regenerative procedure using an enamel matrix derivate. In spite of anticipated poor prognosis, the tooth lesion healed. This case report also discusses the rationale behind different treatment interventions. Practical Implication. Periodontic-endodontic lesions can be successfully treated if dental professionals follow a concerted treatment protocol that integrates endodontic and periodontic specialties. General dentists can be the gatekeepers in managing …

Synthesis And Functional Evaluation Of Peptide Modified Poly (Lactic-Co-Glycolic Acid) Nanoparticles To Inhibit Porphyromonas Gingivalis Biofilm Formation., Paridhi Kalia

Electronic Theses and Dissertations

Periodontal disease is an oral inflammatory disorder that afflicts roughly 46% of the adults in the U.S. Currently, treatment of periodontal disease involves the removal of plaque from the gingival pocket (with possible antibiotic treatment) and if necessary, gingival surgery. To our knowledge, no therapeutic approach exists that promotes host-biofilm homeostasis by limiting pathogen recolonization of the oral cavity after prophylaxis or treatment. The interaction of the pathogen Porphyromonas gingivalis with commensal streptococci is critical for initiation of periodontitis and represents a target for limiting P. gingivalis colonization of the oral cavity. Previous studies showed that a synthetic peptide …

Prevention Of Experimental Periodontitis In Rats With A Simvastatin Prodrug, Aaron Bradley

Theses & Dissertations

Introduction: Simvastatin (SIM) is a hypolipidemic drug that has been shown to have anti-inflammatory and bone anabolic properties. The aim of this study was to examine the effects of novel locally-applied SIM conjugated with methoxypolyethylene glycol to form micelles (SIM/SIM-mPEG) in an experimental periodontitis model in rats using micro-computed tomography (µ-CT) and histologic evaluation.

Methods: Experimental periodontitis was induced using silk ligatures around the maxillary right second molar (M2) in 40 Sprague-Dawley rats. Rats were divided into five groups using SIM/SIM-mPEG at 0.5, 1.0, or 1.5 mg SIM doses, mPEG-alone or no drug (ligature-alone) injected into the palatal M1-M2 gingiva …

Effect Of Chemical Pocket Disinfection As An Adjunct To Non-Surgical Maintenance Therapy Of Inflamed Periodontal Pockets, Chad Riggs

Theses & Dissertations

Purpose: Scaling and root planing with adjunctive chemical pocket disinfection (SRP+C) utilizing sodium hypochlorite solution has been used to treat inflamed pockets of periodontal maintenance patients for many years, without evidence of its benefits. The primary objective was to determine if SRP+C is more effective than scaling and root planing alone (SRP) in improving clinical outcomes. The secondary objective was to compare the effect of SRP+C and SRP on pro-inflammatory IL-1β, anti-inflammatory IL-1ra, and anti-inflammatory index (IL-1ra/IL-1β ratio) found in gingival crevicular fluid (GCF).

Materials and Methods: Pockets (≥ 5 mm and BOP) of 31 maintenance patients were included. Test …

Sustained Release Of Calcium Hydroxide From Poly(Dl-Lactide-Co-Glycolide) Acid Microspheres For Apexification, Bernardino Isaac Cerda-Cristerna, Alejandro Breceda-Leija, Verónica Méndez-González, Daniel Chavarría-Bolaños, Héctor Flores-Reyes, Arturo Garrocho-Rangel, Takashi Komabayashi, Aniket S. Wadajkar, Amaury J. Pozos-Guillén

Dental Medicine Faculty Publications

Calcium hydroxide (Ca(OH)2) loaded poly(DL-lactide-co-glycolide) acid (PLGA) microspheres (MS) might be employed for apexification requiring a sustained release of Ca++. The aim of this study was to formulate and characterize Ca(OH)2-PLGA-MS. The Ca(OH)2-loaded MS were prepared by either oil-in-water (O/W) or water-in-oil/in-water (W/O/W) emulsion solvent evaporation technique. MS produced by the O/W technique exhibited a larger diameter (18.63 ± 7.23 μm) than the MS produced by the W/O/W technique (15.25 ± 7.37 μm) (Mann Whitney U test P < 0.001). The Ca(OH)2 encapsulation efficiency and Ca++ release were calculated from data obtained by absorption techniques. Ca++ release profile was evaluated for 30 days. The percentage of encapsulation efficiency of the O/W-produced MS was higher (24%) than the corresponding percentage of the W/O/W-produced MS (11%). O/W- and W/O/W-produced MS released slower and lower Ca++ than a control Ca(OH)2 paste with polyethylene glycol 400 (ANOVA 1 way, Tukey HSD P < 0.01). O/W-produced MS released higher Ca++ than W/O/W-produced MS (statistically significant differences with t-Student test). We concluded that Ca(OH)2-PLGA-MS were successfully formulated; the technique of formulation influenced on the size, encapsulation efficiency and release profile. The MS were better sustained release system than the Ca(OH)2 paste.

Periodontal Disease: Molecular Visualization Through Computer Modeling And 3d Printing, Catherine Chatowsky

Senior Honors Projects

Periodontal disease is a common infection that damages gingival tissue and creates jawbone destruction that can lead to the loosening or loss of teeth. Hundreds of diverse bacterial species reside in the harsh environment of the oral cavity. The creation of plaque and biofilms serve to allow the bacteria to avoid immune-mediated destruction. This project analyzed periodontal disease pathogens using molecular printing 3D visualization. Two bacterial species (T. denticola and P. gingivalis) were chosen from the public resource: Research Collaboratory for Structural Bioinformatics: Protein Data Bank. T. denticola was a bacteria found to have a binding protein (FhbB) …

Chemokine Function In Periodontal Disease And Oral Cavity Cancer, Sinem Esra Sahingur, W. Andrew Yeudall

Periodontics Publications

The chemotactic cytokines, or chemokines, comprise a superfamily of polypeptides with a wide range of activities that include recruitment of immune cells to sites of infection and inflammation, as well as stimulation of cell proliferation. As such, they function as antimicrobial molecules and play a central role in host defenses against pathogen challenge. However, their ability to recruit leukocytes and potentiate or prolong the inflammatory response may have profound implications for the progression of oral diseases such as chronic periodontitis, where tissue destruction may be widespread. Moreover, it is increasingly recognized that chronic inflammation is a key component of tumor …

The Role Of Leukocyte-Platelet Rich Fibrin In Human Alveolar Ridge Preservation: A Randomized Clinical Trial, Thomas F. Glazier, Thomas C. Waldrop, John C. Gunsolley, Robert Sabatini

Theses and Dissertations

PURPOSE: The aim of this study is to examine the healing of intact extraction sockets grafted with leukocyte-platelet rich fibrin (L-PRF) as compared to sockets grafted with freeze-dried bone allograft (FDBA) and a resorbable collagen barrier membrane (RCM).

METHODS: This prospective randomized, examiner blinded pilot study included 17 subjects randomized to two treatment groups. Serum total cholesterol, low density lipoprotein (LDL), 25-hydroxyvitamin-D₃, and platelet counts were determined preoperatively in all subjects. The experimental arm consisted of 8 posterior tooth-bounded intact extraction sites receiving L-PRF plugs. The control group consisted of 9 posterior tooth-bounded intact extraction sites receiving FDBA …

Peracetic Acid: A Practical Agent For Sterilizing Heat-Labile Polymeric Tissue-Engineering Scaffolds, William R. Trahan

Theses and Dissertations

Advanced biomaterials and sophisticated processing technologies aim to fabricate tissue-engineering scaffolds that can predictably interact within a biological environment at a cellular level. Sterilization of such scaffolds is at the core of patient safety and is an important regulatory issue that needs to be addressed prior to clinical translation. In addition, it is crucial that meticulously engineered micro- and nano- structures are preserved after sterilization. Conventional sterilization methods involving heat, steam and radiation are not compatible with engineered polymeric systems because of scaffold degradation and loss of architecture. Using electrospun scaffolds made from polycaprolactone (PCL), a low melting polymer, and …

Characterization Of Leukocyte-Platelet Rich Fibrin, A Novel Biomaterial, Fadi K. Hasan

Theses and Dissertations

Autologous platelet concentrates represent promising innovative tools in the field of regenerative medicine and are successfully used in oral surgery. Several commercial systems exist that generate various forms of platelet concentrates including Platelet-rich plasma (PRP) and Platelet-rich fibrin (PRF). The alpha- granules of entrapped platelets release a variety of peptide growth factors that promotes healing. Usually PRP is a suspension that can be injected into the site of injury or used as a gel with the addition of thrombin (PRP-gel). In contrast Choukroun’s L-PRF is a dense fibrin based biomaterial enriched with platelets and growth factors. The physical state of …

Cytokine Gene Expression Profiles During Initiation, Progression And Resolution Of Periodontitis, Jeffrey L. Ebersole, Sreenatha S. Kirakodu, Michael John Novak, Arnold J. Stromberg, Shu Shen, Luis Orraca, Janis Gonzalez-Martinez, Armando Burgos, Octavio A. Gonzalez

Center for Oral Health Research Faculty Publications

AIM: Variations in the expression of cytokines during the progression of periodontitis remain ill-defined. We evaluated the expression of 19 cytokine genes related to T-cell phenotype/function during initiation, progression and resolution of periodontitis, and related these to the expression of soft and bone tissue destruction genes (TDGs).

MATERIALS AND METHODS: A ligature-induced periodontitis model was used in rhesus monkeys (M. mulatta) (n = 18). Gingival tissues were taken at baseline pre-ligation, 2 weeks and 1 month (Initiation) and 3 months (progression) post ligation. Ligatures were removed and samples taken 2 months later (resolution). Total RNA was isolated and the Rhesus …

Correlating Periodontal Disease And Cardiovascular Disease: A Comparative Study Of Research Methodology, Alexandra Hensen

Honors Theses

This meta-analysis compares and contrasts three types of population-based research methodology currently used to analyze correlations between Periodontal disease and Cardiovascular disease, the most wide spread diseases in the Western industrialized world. The three studies that were analyzed in this thesis include: "Gender Differences in the Relationship Between Periodontal Disease, Tooth Loss, and Atherosclerosis," published in the Journal of Stroke and Cerebrovascular Diseases (2013), this was a cross-sectional population-based study; "Periodontal Disease and Mortality in Type 2 Diabetes" published in Diabetes Care (2005), this was a longitudinal population-based study; and "Identification of a Shared Genetic Susceptibility Locus for Coronary Heart …

Novel Approach To Assess Periodontal Disease Severity Using Volumetric Analysis, Meera George Maveli

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to evaluate if the absolute change in linear measurements of bone loss on the facial plane of a maxillary central incisor at full and half root length along the vertical axis is directly proportional to volumetric change. The secondary aim was to evaluate if the variation of root volume is influenced by difference in root morphology.

Three dimensional digital models of 70 random patients were obtained from

DICOM CBCT files using volumetric software, InVivoDental (Anatomage, San Jose,

CA). One central incisor of average root length was selected per patient and total length

(L1) and …