The Role Of Mas-Related G-Protein Receptor X2 (Mrgprx2) On Neuropeptide Induced Reponses In Human Mast Cells, 2017 University of Pennsylvania School of Dental Medicine
The Role Of Mas-Related G-Protein Receptor X2 (Mrgprx2) On Neuropeptide Induced Reponses In Human Mast Cells, Wichayapha Manorak
Rationale: Substance P (SP) and hemokinin-1 (HK-1) are neuropeptides (NPs) that promote inflammatory responses by signaling through the neurokinin-1 receptor (NK-1R). Antagonists of NK-1R are highly effective in allergic inflammation and airway hyperresponsiveness in mice but lack efficacy in humans. The reason for this difference is unknown. Human mast cells express both the NK-1R and Mas-related G protein receptor X2 (MRGPRX2) which are both activated by SP. The objective of this study was to determine if HK-1 activates human mast cells via MRGPRX2. Moreover, since MRGPRX2 contains a cholesterol recognition amino acid consensus (CRAC) domain the interaction between MRGPRX2 and ...
P2x7 Receptor Primes Il-1Β And The Nlrp3 Inflammasome In Astrocytes Subjected To Mechanical Strain, 2017 University of Pennsylvania
P2x7 Receptor Primes Il-1Β And The Nlrp3 Inflammasome In Astrocytes Subjected To Mechanical Strain, Farraj S. Albalawi, Wennan Lu, Jonarhan M. Beckel, Jason C. Lim, Stuart A. Mccaughey, Claire H. Mitchell
Inflammatory responses play a key role in many neural pathologies, with localized signaling from non-immune cells making critical contributions. The NLRP3 inflammasome is an important component of innate immune signaling and can link neural insult to chronic inflammation. Stimulation of the NLRP3 inflammasome is a two-stage process. The priming stage involves upregulation of the biosynthesis of the structural components while activation results in their assembly into the actual inflammasome complex and subsequent activation. The priming step can be rate limiting and can connect insult to chronic inflammation but our knowledge of the signals that regulate NLRP3 inflammasome priming in sterile ...
Idiopathic Condylar Resorption: The Current Understanding In Diagnosis And Treatment, 2017 University of the Pacific
Idiopathic Condylar Resorption: The Current Understanding In Diagnosis And Treatment, Andrew L. Young
Andrew L. Young
Idiopathic condylar resorption (ICR) is a condition with no known cause, which manifests as progressive malocclusion, esthetic changes, and often pain. Cone‑beam computed tomography and magnetic resonance imaging are the most valuable imaging methods for diagnosis and tracking, compared to the less complete and more distorted images provided by panoramic radiographs, and the higher radiation of 99mtechnetium‑methylene diphosphonate. ICR has findings that overlap with osteoarthritis, inflammatory arthritis, physiologic resorption/remodeling, congenital disorders affecting the mandible, requiring thorough image analysis, physical examination, and history‑taking. Correct diagnosis and determination of whether the ICR is active or inactive are ...
Caries Classification, 2017 CUNY Hostos Community College
Caries Classification, Diana V. Macri, Annie Chitlall
Publications and Research
It has been 10 years since Featherstone and colleagues presented their evidence-based model of caries management: caries management by risk assessment (CAMBRA). This approach relies on a careful analysis of a patients’ risk factors and protective factors. International and national organizations have long recognized the need for a new caries classification system that incorporates evidence-based approaches to halt progression of caries lesions and minimize the need for surgical intervention. Thus, the International Caries Detection and Assessment System (ICDAS) and the American Dental Association Caries Classification System (ADA CCS) were created. The purpose of this article is to briefly review the ...
Effects Of Palatal Expansion On Speech Production, 2017 Marquette University
Effects Of Palatal Expansion On Speech Production, Jason Milton Meinhardt
Master's Theses (2009 -)
Introduction: Rapid palatal expanders (RPEs) are a commonly used orthodontic adjunct for the treatment of posterior crossbites. RPEs are cemented to bilateral posterior teeth across the palate and thus may interfere with proper tongue movement and linguopalatal contact. The purpose of this study was to identify what specific role RPEs have on speech sound production for the child and early adolescent orthodontic patient. Materials and Methods: RPEs were treatment planned for patients seeking orthodontics at Marquette University. Speech recordings were made using a phonetically balanced reading passage (“The Caterpillar”) at 3 time points: 1) before RPE placement; 2) immediately after ...
Effects Of Mechanical Stretch On Stem Cells From Human Exfoliated Deciduous Teeth (Shed), 2017 University of Pennsylvania
Effects Of Mechanical Stretch On Stem Cells From Human Exfoliated Deciduous Teeth (Shed), Katherine Zhang
No abstract provided.
Establishment Of Innovative Shared Departments To Advance Interdisciplinary Education, 2017 University of New Haven
Establishment Of Innovative Shared Departments To Advance Interdisciplinary Education, Ronald S. Harichandran, Brian Kench, Summer Mcgee, Michael A. Collura, Jean Nocito-Gobel, Charles David Skipton
Engineering and Applied Science Education Faculty Publications
More and more universities are pursuing interdisciplinary academic activities that span across department and college boundaries. Administrative structures to facilitate such programs are difficult to establish within traditional university frameworks consisting of disciplinary departments and colleges. Often interdisciplinary programs are housed in a traditional disciplinary department or college, or in a standalone center reporting to a college dean or the provost. The difficulty of these structures is obtaining broad buy-in from faculty across departments and having disciplinary degree programs include interdisciplinary coursework.
To overcome the difficulties described above, an innovative shared department structure that fosters collaborations to advance interdisciplinary education ...
Palatal Mucosa Derived Fibroblasts Present An Adaptive Behavior Regarding Cytokine Secretion When Grafted Onto The Gingival Margin, Fabíola Pontes Azevedo, Ana Carolina Morandini, Carla Renata Sipert, Thiago José Dionísio, Carlos Ferreira Santos, Carla Andreotti Damante, Maria Lúcia Rubo De Rezende, Adriana Campos Passanezi Sant'ana, Sebastião Luiz Aguiar Greghi
Ana Carolina Morandini
BACKGROUND: Considering that grafted gingival tissue might have to be adapted to the receptor area and that fibroblasts have the ability to respond to bacterial stimuli through the release of various cytokines, this study investigated whether fibroblasts from the palatal mucosa behave differently when grafted onto the gingival margin regarding cytokine secretion. METHODS: Biopsies from the palatal mucosa were collected at the time of free gingival graft surgery, and after four months re-collection was performed upon surgery for root coverage. Fibroblasts were isolated by the explant technique, cultured and stimulated with Porphyromonas gingivalis (Pg) and Escherichia coli (Ec) LPS for ...
Periodontal Ligament And Gingival Fibroblasts Participate In The Production Of Tgf-Β, Interleukin (Il)-8 And Il-10, 2017 University of São Paulo
Periodontal Ligament And Gingival Fibroblasts Participate In The Production Of Tgf-Β, Interleukin (Il)-8 And Il-10, Ana Carolina Morandini, Carla Renata Sipert, Erivan Schnaider Ramos-Junior, Daniel Thomas Brozoski, Carlos Ferreira Santos
Ana Carolina Morandini
The aim of this study was to quantify and compare the production of transforming growth factor beta (TGF-β), interleukin (IL)-8 and IL-10 by human cultured periodontal ligament and gingival fibroblasts both obtained from the same donors challenged with lipopolysaccharide (LPS) from Porphyromonas gingivalis. Fibroblasts were exposed to 0.1-10 µg/mL of LPS from P. gingivalis and after 24 h the supernatants were collected and analyzed by enzyme-linked immunosorbent assay (ELISA). TGF-β protein production was upregulated in a concentration-dependent manner, mainly in gingival fibroblasts, which was statistically significant when challenged by 10 µg/mL LPS. Additionally, at this concentration ...
Functional Local Renin-Angiotensin System In Human And Rat Periodontal Tissue, 2017 University of São Paulo
Functional Local Renin-Angiotensin System In Human And Rat Periodontal Tissue, Carlos Ferreira Santos, Ana Carolina Morandini, Thiago José Dionísio, Flávio A. Faria, Marta C. Lima, Caio M. Figueiredo, Bella L. Colombini-Ishikiriama, Carla Renata Sipert, Rubens P. Maciel, Ana P. Akashi, Gabriela P. Souza, Gustavo P. Garlet, Camila O. Rodini, Sandra L. Amaral, Christiane Becari, Maria C. Salgado, Eduardo B. Oliveira, Isaac Matus, Daniela N. Didier, Andrew S. Greene
Ana Carolina Morandini
The initiation or progression of periodontitis might involve a local renin-angiotensin system (RAS) in periodontal tissue. The aim of this study was to further characterize the local RAS in human and rat periodontal tissues between healthy and periodontally-affected tissue. Components of the RAS were investigated using in vitro, ex vivo and in vivo experiments involving both human and Wistar rat periodontium. Although not upregulated when challenged with P. gingivalis-lipopolysaccharide, human gingival and periodontal ligament fibroblasts expressed RAS components. Likewise, healthy and inflamed human gingiva expressed RAS components, some of which were shown to be functional, yet no differences in expression ...
A Multidisciplinary Treatment Of Congenitally Missing Maxillary Lateral Incisors: A 14-Year Follow-Up Case Report, 2017 University of São Paulo
A Multidisciplinary Treatment Of Congenitally Missing Maxillary Lateral Incisors: A 14-Year Follow-Up Case Report, Renato Rodrigues De Almeida, Ana Carolina Morandini, Renata Rodrigues De Almeida-Pedrin, Marcio Rodrigues De Almeida, Renata Cristina Faria Ribeiro Castro, Natalia Martins Insabralde
Ana Carolina Morandini
Absence of the maxillary lateral incisor creates an aesthetic problem which can be managed in various ways. The condition requires careful treatment planning and consideration of the options and outcomes following either space closure or prosthetic replacement. Recent developments in restorative dentistry have warranted a re-evaluation of the approach to this clinical situation. Factors relating both to the patient and the teeth, including the presentation of malocclusion and the effect on the occlusion must be considered. The objective of this study was to describe the etiology, prevalence and alternative treatment modalities for dental agenesis and to present a clinical case ...
Ccl3 And Cxcl12 Production In Vitro By Dental Pulp Fibroblasts From Permanent And Deciduous Teeth Stimulated By Porphyromonas Gingivalis Lps, Carla Renata Sipert, Ana Carolina Morandini, Karin Cristina Da Silva Modena, Thiago José Dionísio, Maria Aparecida Andrade Moreira Machado, Sandra Helena Penha Oliveira, Ana Paula Campanelli, Carlos Ferreira Santos
Ana Carolina Morandini
OBJECTIVE: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). MATERIAL AND METHODS: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0-10 µg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA ...
Patient Related Outcomes For Dental Implant Therapy With Fixed Prostheses: A Systematic Review, 2017 University of Pennsylvania
Patient Related Outcomes For Dental Implant Therapy With Fixed Prostheses: A Systematic Review, Ahmed M. Kabli
Aim: To explore the available literature compiling studies that discuss patient related outcomes in terms of function and comfort for dental implants with fixed restorations. Materials and Methods: To identify studies relevant to the field of patient related outcomes in terms of function and comfort for treatment with dental implants in fixed restorations, literature review search was done via PubMed, Scopus, Ovid and Cochrane. Review articles were searched for any related studies that could be included in this systematic review. Results: 107 studies were initially identified from the search; 13 studies met the inclusion criteria and were selected for this ...
Foxo1 Deletion Reverses The Effect Of Diabetic-Induced Impaired Fracture Healing, 2017 King Abdulaziz University, Saudi Arabia
Foxo1 Deletion Reverses The Effect Of Diabetic-Induced Impaired Fracture Healing, Mohammed A. Alharbi
Diabetes impairs fracture healing. Our laboratory previously suggested that a dominant mechanism was the premature loss of cartilage during endochondral bone formation. Based on these results we tested the hypothesis that chondrocytes regulate osteoclast formation in diabetes-impaired fracture healing and that it is controlled by the transcription factor FOXO1.
Closed fracture of the femur was induced in mice with lineage-specific FOXO1 deletion in chondrocytes Col2α1Cre+/FOXO1L/L and Col2α1Cre-/ FOXO1L/L as control mice that had FOXO1 gene present. Mice were rendered diabetic by multiple streptozotocin injections. The normoglycemic group received vehicle alone. Specimens were collected at ...
History Of Periodontitis Diagnosis And Edentulism As Predictors Of Cardiovascular Disease, Stroke, And Mortality In Postmenopausal Women, Michael J. Lamonte, Robert J. Genco, Kathleen M. Hovey, Robert B. Wallace, Jo L. Freudenheim, Dominique S. Michaud, Xiaodan Mai, Lesley Tinker, Christian R. Salazar, Christopher A. Andrews, Wenjun Li, Charles Eaton, Lisa W. Martin, Jean Wactawski-Wende
BACKGROUND: Few studies have reported associations between periodontitis and cardiovascular disease (CVD) risk in older women, which is the objective of the present investigation. METHODS AND RESULTS: Participants were 57 001 postmenopausal women ages 55 to 89 years (mean 68 years; > 85% 60 and older) who were enrolled (1993-1998) in the Women's Health Initiative Observational Study, and were without known CVD when history of periodontitis and edentulism was assessed by questionnaire at study Year-5 (1998-2003). There were 3589 incident CVD events and 3816 total deaths during a mean follow-up of 6.7 years. In multivariable analysis, periodontitis was not ...
Expression Of Human Tissue Kallikreins (Klks) In Polymorphous Low Grade Adenocarcinoma (Plga), 2017 The University of Western Ontario
Expression Of Human Tissue Kallikreins (Klks) In Polymorphous Low Grade Adenocarcinoma (Plga), Jacqueline M. Cox
Electronic Thesis and Dissertation Repository
Polymorphous low grade adenocarcinoma (PLGA) is the second most common malignant salivary gland tumour of the minor salivary glands. Human tissue kallikreins (KLKs) are a family of highly conserved serine proteases expressed by various tissues throughout the body. KLKs have become powerful tumour markers for the diagnosis of the cancer patient (e.g. PSA (KLK3)). The literature demonstrates a link between KLKs and salivary gland neoplasms. The purpose of this study is to determine levels of KLK mRNA in tissue samples of formalin fixed paraffin embedded polymorphous low grade adenocarcinoma (PLGA). Secondly, we wish to determine if KLK expression is ...
Todo Lo Que Debes Saber Sobre Los Dientes De Tus Hijos.Pdf, 2017 Selected Works
Todo Lo Que Debes Saber Sobre Los Dientes De Tus Hijos.Pdf, Javier Marco
Porcelain Fused To Metal (Pfm) Crowns And Caries In Adjacent Teeth, 2017 Aga Khan University
Porcelain Fused To Metal (Pfm) Crowns And Caries In Adjacent Teeth, Durr-E-Sadaf, Zubair Ahmad
OBJECTIVE: To assess the discrepancies in marginal integrity and contact points of Porcelain Fused to Metal (PFM) crowns and its association with caries in adjacent teeth. STUDY DESIGN: Cross-sectional study. PLACE AND DURATION OF STUDY: Dental Section of the Aga Khan University Hospital, Karachi, from January to August 2009. METHODOLOGY: Ninety six (96) patients with PFM crowns on maxillary and mandibular premolars and molars were included in this study. Single unit PFM crowns that were part of fixed bridge with at least one natural adjacent tooth were studied. PFM crown with no adjacent tooth or badly broken down teeth were ...
Do Buffered Local Anesthetics Provide More Successful Anesthesia Over Non-Buffered Solutions In Patients Requiring Dental Therapy? – A Systematic Review & Meta-Analysis., 2017 University of Pennsylvania
Do Buffered Local Anesthetics Provide More Successful Anesthesia Over Non-Buffered Solutions In Patients Requiring Dental Therapy? – A Systematic Review & Meta-Analysis., Sereen Kattan, Bekir Karabucak, Elliot V. Hersh, Johnathan M. Korostoff, Paul Hunter
The pH of commercially available local anesthetics (LAs) is purposefully low (pH 3–4). Decreasing the pH extends the shelf life of the solution and prevents its early oxidation. However, a low pH may produce a burning sensation on the injection site, a slower onset of anesthesia, and a decrease in its clinical efficacy. Buffering of local anesthetics (alkalinization) by adding sodium bicarbonate has been suggested to achieve better pain control, reduce the pain of injection and produce a faster onset of local anesthetics. The aim of this review is to utilize a systematic review to collate evidence on ...
Anti-Inflammatory Effect Of Secoisolariciresinol Diglucoside (Sdg) In Microglia, 2017 University of Pennsylvania
Anti-Inflammatory Effect Of Secoisolariciresinol Diglucoside (Sdg) In Microglia, Kevin B. Seto, Kelly L. Jordan-Sciutto, Beth A. Winkelstein, Bekir Karabucak, Eric J. Granquist
Secoisolariciresinol diglucoside (SDG) is the main plant lignan in flaxseed and has been thoroughly researched in the past decades due to its unique health properties. SDG has been shown to have therapeutic benefits for an array of diseases including breast and prostate cancer, hyperlipidemia, atherosclerosis, hypertension, diabetes mellitus, and radiation pneumonopathy. With a number of studies recognizing the various health benefits of SDG, few have focused on its potential anti-inflammatory effect and, specifically, its interaction with the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. Thus, the aim of this study is to elucidate the interaction between SDG and ...