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Palatal Mucosa Derived Fibroblasts Present An Adaptive Behavior Regarding Cytokine Secretion When Grafted Onto The Gingival Margin, Fabíola Pontes Azevedo, Ana Carolina Morandini, Carla Renata Sipert, Thiago José Dionísio, Carlos Ferreira Santos, Carla Andreotti Damante, Maria Lúcia Rubo de Rezende, Adriana Campos Passanezi Sant'ana, Sebastião Luiz Aguiar Greghi 2017 University of São Paulo

Palatal Mucosa Derived Fibroblasts Present An Adaptive Behavior Regarding Cytokine Secretion When Grafted Onto The Gingival Margin, Fabíola Pontes Azevedo, Ana Carolina Morandini, Carla Renata Sipert, Thiago José Dionísio, Carlos Ferreira Santos, Carla Andreotti Damante, Maria Lúcia Rubo De Rezende, Adriana Campos Passanezi Sant'ana, Sebastião Luiz Aguiar Greghi

Ana Carolina Morandini

BACKGROUND: Considering that grafted gingival tissue might have to be adapted to the receptor area and that fibroblasts have the ability to respond to bacterial stimuli through the release of various cytokines, this study investigated whether fibroblasts from the palatal mucosa behave differently when grafted onto the gingival margin regarding cytokine secretion. METHODS: Biopsies from the palatal mucosa were collected at the time of free gingival graft surgery, and after four months re-collection was performed upon surgery for root coverage. Fibroblasts were isolated by the explant technique, cultured and stimulated with Porphyromonas gingivalis (Pg) and Escherichia coli (Ec) LPS for ...


Periodontal Ligament And Gingival Fibroblasts Participate In The Production Of Tgf-Β, Interleukin (Il)-8 And Il-10, Ana Carolina Morandini, Carla Renata Sipert, Erivan Schnaider Ramos-Junior, Daniel Thomas Brozoski, Carlos Ferreira Santos 2017 University of São Paulo

Periodontal Ligament And Gingival Fibroblasts Participate In The Production Of Tgf-Β, Interleukin (Il)-8 And Il-10, Ana Carolina Morandini, Carla Renata Sipert, Erivan Schnaider Ramos-Junior, Daniel Thomas Brozoski, Carlos Ferreira Santos

Ana Carolina Morandini

The aim of this study was to quantify and compare the production of transforming growth factor beta (TGF-β), interleukin (IL)-8 and IL-10 by human cultured periodontal ligament and gingival fibroblasts both obtained from the same donors challenged with lipopolysaccharide (LPS) from Porphyromonas gingivalis. Fibroblasts were exposed to 0.1-10 µg/mL of LPS from P. gingivalis and after 24 h the supernatants were collected and analyzed by enzyme-linked immunosorbent assay (ELISA). TGF-β protein production was upregulated in a concentration-dependent manner, mainly in gingival fibroblasts, which was statistically significant when challenged by 10 µg/mL LPS. Additionally, at this concentration ...


A Multidisciplinary Treatment Of Congenitally Missing Maxillary Lateral Incisors: A 14-Year Follow-Up Case Report, Renato Rodrigues de Almeida, Ana Carolina Morandini, Renata Rodrigues de Almeida-Pedrin, Marcio Rodrigues de Almeida, Renata Cristina Faria Ribeiro Castro, Natalia Martins Insabralde 2017 University of São Paulo

A Multidisciplinary Treatment Of Congenitally Missing Maxillary Lateral Incisors: A 14-Year Follow-Up Case Report, Renato Rodrigues De Almeida, Ana Carolina Morandini, Renata Rodrigues De Almeida-Pedrin, Marcio Rodrigues De Almeida, Renata Cristina Faria Ribeiro Castro, Natalia Martins Insabralde

Ana Carolina Morandini

Absence of the maxillary lateral incisor creates an aesthetic problem which can be managed in various ways. The condition requires careful treatment planning and consideration of the options and outcomes following either space closure or prosthetic replacement. Recent developments in restorative dentistry have warranted a re-evaluation of the approach to this clinical situation. Factors relating both to the patient and the teeth, including the presentation of malocclusion and the effect on the occlusion must be considered. The objective of this study was to describe the etiology, prevalence and alternative treatment modalities for dental agenesis and to present a clinical case ...


Ccl3 And Cxcl12 Production In Vitro By Dental Pulp Fibroblasts From Permanent And Deciduous Teeth Stimulated By Porphyromonas Gingivalis Lps, Carla Renata Sipert, Ana Carolina Morandini, Karin Cristina da Silva Modena, Thiago José Dionísio, Maria Aparecida Andrade Moreira Machado, Sandra Helena Penha Oliveira, Ana Paula Campanelli, Carlos Ferreira Santos 2017 University of São Paulo

Ccl3 And Cxcl12 Production In Vitro By Dental Pulp Fibroblasts From Permanent And Deciduous Teeth Stimulated By Porphyromonas Gingivalis Lps, Carla Renata Sipert, Ana Carolina Morandini, Karin Cristina Da Silva Modena, Thiago José Dionísio, Maria Aparecida Andrade Moreira Machado, Sandra Helena Penha Oliveira, Ana Paula Campanelli, Carlos Ferreira Santos

Ana Carolina Morandini

OBJECTIVE: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). MATERIAL AND METHODS: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0-10 µg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA ...


Functional Local Renin-Angiotensin System In Human And Rat Periodontal Tissue, Carlos Ferreira Santos, Ana Carolina Morandini, Thiago José Dionísio, Flávio A. Faria, Marta C. Lima, Caio M. Figueiredo, Bella L. Colombini-Ishikiriama, Carla Renata Sipert, Rubens P. Maciel, Ana P. Akashi, Gabriela P. Souza, Gustavo P. Garlet, Camila O. Rodini, Sandra L. Amaral, Christiane Becari, Maria C. Salgado, Eduardo B. Oliveira, Isaac Matus, Daniela N. Didier, Andrew S. Greene 2017 University of São Paulo

Functional Local Renin-Angiotensin System In Human And Rat Periodontal Tissue, Carlos Ferreira Santos, Ana Carolina Morandini, Thiago José Dionísio, Flávio A. Faria, Marta C. Lima, Caio M. Figueiredo, Bella L. Colombini-Ishikiriama, Carla Renata Sipert, Rubens P. Maciel, Ana P. Akashi, Gabriela P. Souza, Gustavo P. Garlet, Camila O. Rodini, Sandra L. Amaral, Christiane Becari, Maria C. Salgado, Eduardo B. Oliveira, Isaac Matus, Daniela N. Didier, Andrew S. Greene

Ana Carolina Morandini

The initiation or progression of periodontitis might involve a local renin-angiotensin system (RAS) in periodontal tissue. The aim of this study was to further characterize the local RAS in human and rat periodontal tissues between healthy and periodontally-affected tissue. Components of the RAS were investigated using in vitro, ex vivo and in vivo experiments involving both human and Wistar rat periodontium. Although not upregulated when challenged with P. gingivalis-lipopolysaccharide, human gingival and periodontal ligament fibroblasts expressed RAS components. Likewise, healthy and inflamed human gingiva expressed RAS components, some of which were shown to be functional, yet no differences in expression ...


History Of Periodontitis Diagnosis And Edentulism As Predictors Of Cardiovascular Disease, Stroke, And Mortality In Postmenopausal Women, Michael J. LaMonte, Robert J. Genco, Kathleen M. Hovey, Robert B. Wallace, Jo L. Freudenheim, Dominique S. Michaud, Xiaodan Mai, Lesley Tinker, Christian R. Salazar, Christopher A. Andrews, Wenjun Li, Charles Eaton, Lisa W. Martin, Jean Wactawski-Wende 2017 SUNY University at Buffalo

History Of Periodontitis Diagnosis And Edentulism As Predictors Of Cardiovascular Disease, Stroke, And Mortality In Postmenopausal Women, Michael J. Lamonte, Robert J. Genco, Kathleen M. Hovey, Robert B. Wallace, Jo L. Freudenheim, Dominique S. Michaud, Xiaodan Mai, Lesley Tinker, Christian R. Salazar, Christopher A. Andrews, Wenjun Li, Charles Eaton, Lisa W. Martin, Jean Wactawski-Wende

Wenjun Li

BACKGROUND: Few studies have reported associations between periodontitis and cardiovascular disease (CVD) risk in older women, which is the objective of the present investigation. METHODS AND RESULTS: Participants were 57 001 postmenopausal women ages 55 to 89 years (mean 68 years; > 85% 60 and older) who were enrolled (1993-1998) in the Women's Health Initiative Observational Study, and were without known CVD when history of periodontitis and edentulism was assessed by questionnaire at study Year-5 (1998-2003). There were 3589 incident CVD events and 3816 total deaths during a mean follow-up of 6.7 years. In multivariable analysis, periodontitis was not ...


Todo Lo Que Debes Saber Sobre Los Dientes De Tus Hijos.Pdf, Javier Marco 2017 Selected Works

Todo Lo Que Debes Saber Sobre Los Dientes De Tus Hijos.Pdf, Javier Marco

Javier Marco

¿Queréis descubrir algunos de los falsos mitos más famosos de la salud y la higiene dental entorno a la odontopediatria? Para el cuidado dental de los más pequeño los mas importante es generar buenas costumbres para la prevención de enfermedades y problemas bucales. ¡Sigue leyendo!
 


Porcelain Fused To Metal (Pfm) Crowns And Caries In Adjacent Teeth, Durr-E-Sadaf, Zubair Ahmad 2017 Aga Khan University

Porcelain Fused To Metal (Pfm) Crowns And Caries In Adjacent Teeth, Durr-E-Sadaf, Zubair Ahmad

Zubair Ahmad

OBJECTIVE: To assess the discrepancies in marginal integrity and contact points of Porcelain Fused to Metal (PFM) crowns and its association with caries in adjacent teeth. STUDY DESIGN: Cross-sectional study. PLACE AND DURATION OF STUDY: Dental Section of the Aga Khan University Hospital, Karachi, from January to August 2009. METHODOLOGY: Ninety six (96) patients with PFM crowns on maxillary and mandibular premolars and molars were included in this study. Single unit PFM crowns that were part of fixed bridge with at least one natural adjacent tooth were studied. PFM crown with no adjacent tooth or badly broken down teeth were ...


Do Buffered Local Anesthetics Provide More Successful Anesthesia Over Non-Buffered Solutions In Patients Requiring Dental Therapy? – A Systematic Review & Meta-Analysis., Sereen Kattan, Bekir Karabucak, Elliot V. Hersh, Johnathan M. Korostoff, Paul Hunter 2017 University of Pennsylvania

Do Buffered Local Anesthetics Provide More Successful Anesthesia Over Non-Buffered Solutions In Patients Requiring Dental Therapy? – A Systematic Review & Meta-Analysis., Sereen Kattan, Bekir Karabucak, Elliot V. Hersh, Johnathan M. Korostoff, Paul Hunter

Dental Theses

Background:

The pH of commercially available local anesthetics (LAs) is purposefully low (pH 3–4). Decreasing the pH extends the shelf life of the solution and prevents its early oxidation. However, a low pH may produce a burning sensation on the injection site, a slower onset of anesthesia, and a decrease in its clinical efficacy. Buffering of local anesthetics (alkalinization) by adding sodium bicarbonate has been suggested to achieve better pain control, reduce the pain of injection and produce a faster onset of local anesthetics. The aim of this review is to utilize a systematic review to collate evidence on ...


Anti-Inflammatory Effect Of Secoisolariciresinol Diglucoside (Sdg) In Microglia, Kevin B. Seto, Kelly L. Jordan-Sciutto, Beth A. Winkelstein, Bekir Karabucak, Eric J. Granquist 2017 University of Pennsylvania

Anti-Inflammatory Effect Of Secoisolariciresinol Diglucoside (Sdg) In Microglia, Kevin B. Seto, Kelly L. Jordan-Sciutto, Beth A. Winkelstein, Bekir Karabucak, Eric J. Granquist

Dental Theses

Secoisolariciresinol diglucoside (SDG) is the main plant lignan in flaxseed and has been thoroughly researched in the past decades due to its unique health properties. SDG has been shown to have therapeutic benefits for an array of diseases including breast and prostate cancer, hyperlipidemia, atherosclerosis, hypertension, diabetes mellitus, and radiation pneumonopathy. With a number of studies recognizing the various health benefits of SDG, few have focused on its potential anti-inflammatory effect and, specifically, its interaction with the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. Thus, the aim of this study is to elucidate the interaction between SDG and ...


Got Teeth? How The Oral Microbiome And Diet Affects Our Oral Health And The Future Of Dentistry, Joellen Coates 2017 University of Wyoming

Got Teeth? How The Oral Microbiome And Diet Affects Our Oral Health And The Future Of Dentistry, Joellen Coates

Honors Theses AY 16/17

Within the last decade, the human microbiome, especially the gut and oral microbiomes, has been heavily researched to understand its relationship to our health and its diversity. The oral microbiome has become of increasing interest in the last few years to determine how the microbiome affects oral health and the presence of dental diseases, such as dental caries (cavities) and periodontitis. It has been suggested that the oral microbiome is in a state of imbalance, or dysbiosis, during oral diseases and can be affected by several factors, such as genetics, oral hygiene habits, and more importantly diet. Ancient dental remains ...


Dental Disparities And The Safety Net In Blount County, Micaela J. Cornett 2017 East Tennessee State University

Dental Disparities And The Safety Net In Blount County, Micaela J. Cornett

Undergraduate Honors Theses

This qualitative study focused on the dental disparities in Blount County, TN and sought to determine if there are enough dental clinics within the dental safety net. Interviews were conducted with 18 individuals who were either service providers or clients of organizations such as the Salvation Army, Alcoa Good Samaritan Clinic, the local health department, Trinity Dental Clinic, Volunteer Ministry Center, Remote Area Medical, and Blount Memorial Hospital. Inclusion criteria for clients included: homeless or living below the poverty level, uninsured, 18 to 65 years of age, has not seen a dentist in the past year and currently suffering a ...


Worldwide Use Of Triclosan: Can Dentistry Do Without This Antimicrobial?, Diana V. Macri 2017 CUNY Hostos Community College

Worldwide Use Of Triclosan: Can Dentistry Do Without This Antimicrobial?, Diana V. Macri

Publications and Research

No abstract provided.


Enhancing Nursing Students’ Understanding Of Oral Health: An Educational Intervention With An Interprofessional Component, Shelley Spurr Dr., Jill Bally Dr., Alyssa Hayes Dr., Marcella Ogenchuk Dr., Krista Trinder 2017 University of Saskatchewan, College of Nursing

Enhancing Nursing Students’ Understanding Of Oral Health: An Educational Intervention With An Interprofessional Component, Shelley Spurr Dr., Jill Bally Dr., Alyssa Hayes Dr., Marcella Ogenchuk Dr., Krista Trinder

Quality Advancement in Nursing Education - Avancées en formation infirmière

Oral health is integral to general health and essential for well-being, and therefore, should be prioritized in pediatric nursing education. The purpose of this pilot study was to examine if an oral health education intervention with an Interprofessional Education (IPE) component delivered to third-year baccalaureate nursing students would improve their knowledge of pediatric oral health care. Nursing students (n=99) from a Bachelor of Nursing program in a mid-Western Canadian university completed a survey before and after receiving the educational intervention which included a two-hour lecture from a Dentistry faculty member and a one-hour clinical lab in which nursing students ...


Viability Of Human Periodontal Ligament Fibroblasts After Time In Tooth Storage Media, Scott MacDonald 2017 Marquette University

Viability Of Human Periodontal Ligament Fibroblasts After Time In Tooth Storage Media, Scott Macdonald

Master's Theses (2009 -)

Periodontal ligament cell viability at the time of tooth replantation is of utmost importance for proper repair of the damaged tissues (1). Multiple different options are currently available for storage of an avulsed tooth. The goal of this study was to investigate new storage media and evaluate their ability to maintain cell viability over time. Materials and Methods: Cultured human periodontal ligament fibroblasts (hPDLF) were seeded on 96-well plates. These cells were exposed to different storage media including: Hank’s Balanced Salt Solution (HBSS), EMT Toothsaver (EMT), Save-A-Tooth (SAT), trehalose (T300), cell culture media, water, and different formulations of HBSS ...


History Of Periodontitis Diagnosis And Edentulism As Predictors Of Cardiovascular Disease, Stroke, And Mortality In Postmenopausal Women, Michael J. LaMonte, Robert J. Genco, Kathleen M. Hovey, Robert B. Wallace, Jo L. Freudenheim, Dominique S. Michaud, Xiaodan Mai, Lesley Tinker, Christian R. Salazar, Christopher A. Andrews, Wenjun Li, Charles Eaton, Lisa W. Martin, Jean Wactawski-Wende 2017 SUNY University at Buffalo

History Of Periodontitis Diagnosis And Edentulism As Predictors Of Cardiovascular Disease, Stroke, And Mortality In Postmenopausal Women, Michael J. Lamonte, Robert J. Genco, Kathleen M. Hovey, Robert B. Wallace, Jo L. Freudenheim, Dominique S. Michaud, Xiaodan Mai, Lesley Tinker, Christian R. Salazar, Christopher A. Andrews, Wenjun Li, Charles Eaton, Lisa W. Martin, Jean Wactawski-Wende

University of Massachusetts Medical School Faculty Publications

BACKGROUND: Few studies have reported associations between periodontitis and cardiovascular disease (CVD) risk in older women, which is the objective of the present investigation.

METHODS AND RESULTS: Participants were 57 001 postmenopausal women ages 55 to 89 years (mean 68 years; > 85% 60 and older) who were enrolled (1993-1998) in the Women's Health Initiative Observational Study, and were without known CVD when history of periodontitis and edentulism was assessed by questionnaire at study Year-5 (1998-2003). There were 3589 incident CVD events and 3816 total deaths during a mean follow-up of 6.7 years. In multivariable analysis, periodontitis was not ...


Oral Health Beliefs Of Alaska Native Dental Patients, Abigail N. Adams, Carly T. McKenzie 2017 University of Alabama - Birmingham

Oral Health Beliefs Of Alaska Native Dental Patients, Abigail N. Adams, Carly T. Mckenzie

Journal on Race, Inequality, and Social Mobility in America

This study investigated the oral health beliefs of Alaska Native dental patients via a questionnaire with 4-point Likert scales. Respondents (n = 136) reported high perceived importance of keeping natural teeth. Females viewed oral health as more important than males. Respondents generally did not view dentists as readily available (M = 2.86, SD = 0.66). Perceived availability and efficacy of dentists were positively correlated (r = 0.219, p = 0.021). Beliefs regarding seriousness of dental problems and importance of oral health were strongly correlated (r = 0.547, p < 0.001). Age predicted perceived seriousness of oral health problems, dentist availability, and prevention benefits. Access to dental care is perceived as a barrier among Alaska Natives generally and elders in particular. Oral health education and prevention messages should utilize the existing belief that keeping natural teeth is important. Public information about available services and transportation logistics may decrease perceived availability barriers.


Interview With Ralph Merrill, D.D.S., M.Sc.D., Ralph Merrill D.D.S., M.Sc.D. (interviewee), Louis G. Terkla D.M.D. (interviewer) 2017 Oregon Health & Science University

Interview With Ralph Merrill, D.D.S., M.Sc.D., Ralph Merrill D.D.S., M.Sc.D. (Interviewee), Louis G. Terkla D.M.D. (Interviewer)

Oral History Collection

Ralph Merrill, D.D.S., M.Sc.D., was born in Salt Lake City on December 24, 1932. He decided on a career in dentistry while studying at the University of Denver and during an externship at a Native American hospital in Alaska, his mentor's influence guided him into focusing on surgery. He attended the University of Washington School of Dentistry, completing residencies at Boston City Hospital, Boston University, and Henry Ford Hospital (Detroit). Merrill came to OHSU as he wished to work on the academic side of oral surgery and was particularly impressed with the ortho-pathology departments and ...


Correlation Between Numbers Of Cells In Human Dental Pulp And Age Implications For Age Estimation.Pdf, Mohammad Zakir Hossain, Sulinda Daud, Phrabkaran Nambiar, Fathilah Abdul Razak, Norintan Ab Murat, Roslan Saub, Marina Mohd Bakri 2017 Faculty of Dentistry, University of Malaya

Correlation Between Numbers Of Cells In Human Dental Pulp And Age Implications For Age Estimation.Pdf, Mohammad Zakir Hossain, Sulinda Daud, Phrabkaran Nambiar, Fathilah Abdul Razak, Norintan Ab Murat, Roslan Saub, Marina Mohd Bakri

Marina Mohd Bakri

OBJECTIVE:
The aim of this study was to investigate correlations between dental pulp cell count of odontoblasts, subodontoblasts and fibroblasts and age, within different age groups. Formulation of regression equations using the dental pulp cell count for predicting age was attempted.

DESIGN:
Eighty-one extracted teeth were grouped into two age groups (6-25 years, 26-80 years). The teeth were demineralized and histological sections were prepared for cell count. Regression equations were generated from regression analysis of cell count and tested for age estimation.

RESULTS:
The number of dental pulp cells were found to increase until around the third decade of life ...


Recurrent Rearrangements Of The Myb/Sant-Like Dna-Binding Domain Containing 3 Gene (Msantd3) In Salivary Gland Acinic Cell Carcinoma, Nicholas Barasch, Xue Gong, Kevin A. Kwei, Sushama Varma, Jewison Biscocho, Kunbin Qu, Nan (Tori) Xiao, Joseph S. Lipsick, Robert J. Pelham, Robert B. West, Jonathan R. Pollack 2017 University of the Pacific

Recurrent Rearrangements Of The Myb/Sant-Like Dna-Binding Domain Containing 3 Gene (Msantd3) In Salivary Gland Acinic Cell Carcinoma, Nicholas Barasch, Xue Gong, Kevin A. Kwei, Sushama Varma, Jewison Biscocho, Kunbin Qu, Nan (Tori) Xiao, Joseph S. Lipsick, Robert J. Pelham, Robert B. West, Jonathan R. Pollack

Dugoni School of Dentistry Faculty Articles

Pathogenic gene fusions have been identified in several histologic types of salivary gland neoplasia, but not previously in acinic cell carcinoma (AcCC). To discover novel gene fusions, we performed whole-transcriptome sequencing surveys of three AcCC archival cases. In one specimen we identified a novel HTN3-MSANTD3 gene fusion, and in another a novel PRB3-ZNF217 gene fusion. The structure of both fusions was consistent with the promoter of the 5' partner (HTN3 or PRB3), both highly expressed salivary gland genes, driving overexpression of full-length MSANTD3 or ZNF217. By fluorescence in situ hybridization of an expanded AcCC case series, we observed MSANTD3 rearrangements ...


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