Oral Biology and Oral Pathology Commons^™

The Effect Of Cheese On The Ph Levels In The Oral Cavity, Madison R. Hayden

Mahurin Honors College Capstone Experience/Thesis Projects

In the Fall of 2014, research was conducted with 20 participants on the campus of Western Kentucky University. Participants were given two ounces of Coca Cola^® to drink and the pH level in their mouth was tested one minute later. After waiting a minute, the participants were given a one ounce square of mild cheddar cheese to eat and the pH level was tested again one minute later. For further study, this project will be expanded to include more subjects as well as an increased exposure time to Coca Cola^®. This research is conducted to help support …

Oral Wellness: Using Occupational Therapy To Enhance Oral Hygiene Delivery In Long-Term Care, Lauryn J. Banovitz, Liberty Bellah, Rosemarie Lion

Dissertations, Masters Theses, Capstones, and Culminating Projects

Based on current literature, oral health in long-term care (LTC) facilities is frequently of low priority and does not follow evidence-based best practices. Poor oral health reduces the quality of life of older adult residents and patients and can lead to systemic diseases, such as cardiovascular disease, stroke, and pneumonia, the leading cause of death in LTC. Occupational therapists can play an instrumental role as oral care consultants, and educators, and can act as resource guides to raise the standards of oral care in LTC facilities.

This capstone project explored ways in which occupational therapy, a profession traditionally designated for …

Identification Of Transcription Factors Gzf3, Rfx1, Orf19.3928 As Being Implicated In Candida-Bacterial Interactions., Joni Watson

Undergraduate Honors Theses

Candida albicans is an opportunistic pathogen that is present in the normal flora in a majority of individuals. One key factor in C. albicans virulence is the ability to change its morphology from yeast to an elongated or hyphal form. The regulation of this morphogenesis relies in part upon quorum sensing (QS) molecules. C. albicans often exists as part of a mixed culture alongside other microbes and is influenced by their presence as well as the presence of QS molecules that they produce. In this study, a library of diploid homozygous transcriptional regulator knockout (TRKO) mutants were screened to identify …

Evaluating The Marginal Integrity Of Lithium Disilicate Veneers Fabricated By Digital Impressions And Cad/Cam Compared To Conventional Techniques., Michael C. Guzelian

Electronic Theses and Dissertations

This in vitro study compared marginal gap size in anterior lithium disilicate veneers produced by conventional and digital impressions. One typodont right central incisor was prepared for an all-ceramic cast. Ten conventional veneers were fabricated using Type IV stone, PVS, and IPS e.max press, while ten digital veneers were fabricated using Lava COS (3M ESPE) and IPS e.max CAD/CAM processing and milling. Samples were divided double-blindly, captured at 45X magnification, evaluated at three images per orientation (B-D-M-P), and measured at three distances (largest, smallest, best fit) per image. Data points were entered into SPSS code for one-way and two-way ANOVA, …

Vitamin A Is Essential For Proper Embryonic Submandibular Salivary Gland Growth., Deanna Ellene Buenger

Electronic Theses and Dissertations

Submandibular salivary glands (SMG) are important for the production of saliva. Salivary glands may be damaged by autoimmune disease, surgery, or radiation therapy. Retinoic acid (RA) is a signaling metabolite derived from Vitamin A that is essential for proper embryonic growth and development; specifically for cardiovascular, limb and craniofacial development. The goal of this study was to determine if there is a reproducible defect in the growth of SMGs in RA deficient mouse embryos compared to wild type. This study aims to characterize SMG growth in RA deficient embryos and determine if the growth could be stimulated by RA in …

Electronic Cigarettes: A New Generation Of Smoking, Claire M. Warren

Mahurin Honors College Capstone Experience/Thesis Projects

Electronic cigarette smoking is a trend that has become very popular in the United States in recent years. As e-cigarettes continue to gain popularity, it is important for dental professionals to be up-to-date on the dangers that they pose.

An interview session consisting of a questionnaire was the methodology used in this study. Ten dental hygienists who work full or part-time in private practice settings were interviewed. After the interview questions were asked, the researcher discussed the effects of electronic cigarettes can have on oral and overall health. Last, the data were analyzed to determine if dental hygienists included electronic …

Giant Cell Tumor Of The Larynx Treated By Surgery And Adjuvant Denosumab: Case Report And Review Of The Literature, A. E. Yancoskie, D. K. Frank, J. E. Fantasia, S. Savona, N. Eiseler, I. Reder, L. B. Kahn

Journal Articles

Giant cell tumor of the larynx (GCTL) is a rare entity; only 34 cases have been reported in the literature. We report a case of GCTL in a 46 year-old male presenting clinical, radiographic, histological and therapeutic features. Previously reported cases are also reviewed.

Immunohistochemical Assessment Of Cytokeratin 17 And Epcam Expression In Different Grades Of Oral Squamous Cell Carcinoma, Safaa El-Baz, Iman Helmy, Mohamed Hosam El Mallahy

Dentistry

Oral squamous cell carcinoma (OSCC), one of the most common malignancies in the head and neck region, is graded based on Broder’s classification into well-differentiated, moderately, and poorly differentiated. Cytokeratin 17 (K17, CK17) is an acid-type I cytokeratin with a low-molecular weight of 48.1 kDa. Epithelial cell adhesion molecule (EpCAM) is a hemophilic Ca2+ independent adhesion molecule. This study used an immunohistochemical technique (IHC) to examine the expression of CK17 and EpCAM in thirty-two specimens of OSCC (comprising 13 well-, 9 moderately, and 10 poorly differentiated cases), and 2 specimens of normal oral mucosa, used as control. It was found …

The Expression And Potential Significance Of Human Kallikreins 6, 7, 8, 10, 13, And 14 In The Epithelium Of Selected Odontogenic Cysts And Tumors Of Variably Aggressive Biological Behaviour, Rebecca Woodford

Electronic Thesis and Dissertation Repository

ABSTRACT

This study analyzed the immunohistochemical staining, localization and relative concentrations of human kallikreins (KLKs) 6,7,8,10,13,14 to determine if there is a predilection for odontogenic epithelium and if differential staining could predict biological behavior of selected odontogenic lesions.

We found that all KLKs studied were present in the epithelium of all lesions examined. However, there were significant differences in location of intra-epithelial staining and staining scores between the lesions. There was no significant, unexplainable difference in staining between odontogenic cysts and the control cyst indicating no specific role in odontogenic cystogenesis. However, the odontogenic neoplasms showed statistically significant increased expression …

Interleukin-6 Production And Epithelial Cell Signaling By Telomerase Immortalized Gingival Keratinocytes In Porphyromonas Gingivalis Infection., Himabindu Vuddaraju

Electronic Theses and Dissertations

P. gingivalis is a major periodontal disease causing pathogen. Gingival epithelial cells being the first line of defense, interact and internalize the bacteria evoking a cell signaling response that finally leads to the release of cytokines. IL-6 is a potent pro- inflammatory cytokine, which is one of the responsible factors for bone resorption, a clinical manifestation of periodontitis. The experimental model study hypothesizes that P. gingivalis infection increases the production of IL-6 in telomerase immortalized gingival keratinocytes (TIGKs) through up-regulation of c-Jun (AP-1 reporter). We observed the IL-6 production post-infection with P. gingivalis WT ATCC33277 and mutants ?0482, ?Ndk and …

Cholinergic Inhibition Of Lipopolysaccharide Induced Matrix Metalloproteinase In Immune Cells., Harrison Black,

Electronic Theses and Dissertations

Background: Matrix metalloproteinase (MMPs) are proteins that have a unique role in immunity, tissue remodeling, and tumorigenesis. MMPs can break down extracellular components, release bioactive molecules, and act on pro-inflammatory cytokines, chemokines and other proteins to regulate varied aspects of inflammation and immunity. Nicotine (3-(1-methyl-2-pyrrolidinyl) pyridine), a key toxic component of tobacco, is thought to dysregulate matrix metalloproteinase secretion in immune cells. Aim: Examine the effect of nicotine on MMP9 secretion and activity in primary monocytes. Methods: Primary monocytes were stimulated with LPS (1 µg/ml) and Nicotine (100 ng/ml) and MMP9 secretion were studied. Gel zymography and ELISA techniques were …

Chemical Dental Plaque Control: Chlorhexidine Tooth Staining And Efficacy Of Common Whitening Procedures, Zoe Kiklis

Scripps Senior Theses

Chlorhexidine mouth rinses remove dental plaque from teeth, preventing dental caries, dental decay, and more serious systemic infections. Tooth discoloration due to extrinsic staining is the most prominent side effect of regular chlorhexidine use. Decreasing the concentration of chlorhexidine reduces staining area and severity. Staining can also be prevented and treated by tooth bleaching, a common cosmetic dental procedure that diminishes extrinsic staining by oxidizing chromagens adsorbed onto the tooth surface. In the proposed trial, common bleaching agents will be investigated for their efficacies in preventing chlorhexidine staining. Results of the trial could further the development of a chlorhexidine mouth …

Effect Of Metallic Restoration Artifacts On Maxillofacial Cone Beam Computed Tomography Images., Mitali Binani 1987-

Electronic Theses and Dissertations

Artifacts due to high-density objects (HDO) such as metallic dental restorations on maxillofacial cone beam computed tomographic (CBCT) images can render certain areas unsuitable for diagnosis. It was hypothesized that image quality due to HDO artifacts was affected by CBCT acquisition parameters and the number and configuration of HDO. Simulated complete maxillary and mandibular dental arches were constructed using dental stone and extracted teeth. Conservative coronal dental amalgam (MOD) restorations on premolar and molar teeth were used as HDOs. Gray values (GV) measured on uniform dental stone test cylinders at specific levels from the occlusal plane at three tooth locations …

Neutrophils Alter Epithelial Response To Porphyromonas Gingivalis In A Gingival Crevice Model., Jessica Lynn Bondy-Carey 1987-

Electronic Theses and Dissertations

A gingival crevice model (epithelial cell-Porphyromonas gingivalis-neutrophil) was established and used to profile gingipain, matrix metalloproteinase (MMP), MMP mediators [neutrophil gelatinase-associated lipocalin (NGAL) and tissue inhibitor of metalloproteinases 1 (TIMP-1)] and cytokine networks. Smoking is the primary environmental risk factor for periodontitis. Therefore, the influence of cigarette smoke extract (CSE) was also monitored in the same model. Porphyromonas gingivalis alone induced low levels of interleukin-1b and interleukin-8 from epithelial cells, but high levels of both cytokines were produced on the addition of neutrophils. Exposure to CSE (100 and 1000 ng mL1 nicotine equivalency) significantly compromised P. gingivalis-induced cytokine secretion (both …

Clinical Efficacy Of Cross-Sectional Imaging Compared With Panoramic Imaging And Virtual 3d Models For The Assessment Of Dental Implant Placement., Moiz Ahmad Khan 1987-

Electronic Theses and Dissertations

Introduction: This study compared the clinical efficacy of panoramic imaging and Cone Beam Computed Tomography (CBCT) in the optimal placement of virtual dental implants in the posterior edentulous bounded regions of the jaws. Material and methods: From a retrospective audit of patient records, fifty-two subjects were recruited with sixty-one dental implant sites in the maxilla and mandible. Physical measurements of the residual alveolar ridge were performed and consensus decisions of optimal implant length and bone graft necessity were obtained using reformatted panoramic alone (RP) or cross-sectional imaging (XS). Results: Horizontal restorative space measurements greater for RP (p=0.001). Shorter implants were …

Implementation Of An Oral Health Intervention For Adults With Intellectual And Developmental Disabilities., Brigit Rene Zaksek

Electronic Theses and Dissertations

Often oral care is overlooked during the daily care of adults with intellectual and developmental disabilities (IDD). Due to the integration of adults with IDD from institutions into group homes and private homes, caregivers need to be taught to implement daily oral hygiene protocols. The purpose of this pilot study, conducted by the University of Louisville and the Pacific Institute for Research and Evaluation, was to develop an oral hygiene plan for adults with IDD residing in group homes in the Louisville, Kentucky area that would ultimately improve their oral health and quality of life. This thesis focuses on the …

The Influence Of Vitamin D Metabolism On Gene Expression, Matrix Production And Mineralization During Osteoprecursor Cell-Based Bone Development, Shelley S. Mason, Sean S. Kohles, Shelley R. Winn, Randy D. Zelick

Biology Faculty Publications and Presentations

Background: Multipotential precursor cell lines derived from human bone marrow, capable of differentiating into cartilage or bone, may provide a useful tissue development model for studying the regulation and metabolism of putative growth and differentiation factors necessary for tissue regeneration. In mammals, the process of bone development depends on the proliferation and differentiation of osteoblast lineage cells, and the subsequent synthesis and mineralization of bone extracellular matrix (ECM). Vitamin D metabolites play a pivotal role in bone and mineral homeostasis, and are positive factors on bone development. Recently, it was demonstrated that a human-derived engineered osteoblast precursor cell line (OPC1), …

Adult Scurvy In New France: Samuel De Champlain's "Mal De La Terre" At Saint Croix Island, 1604-1605, Thomas A. Crist, Marcella H. Sorg

Anthropology Faculty Scholarship

No abstract provided.

Fluorescent Technology Versus Visual And Tactile Examination In The Detection Of Oral Lesion: A Pilot Study, Hadeel Mohammed Ayoub

Dental Hygiene Theses & Dissertations

Purpose: The purpose of the study was to compare the effectiveness of the VELscope® Vx, versus a visual and tactile intraoral examination in detecting oral lesions in an adult, high risk population. Methods: A convenience sample of 30 participants (17 cigarette smokers and 13 dual addiction smokers) was enrolled. For the purpose of this study, dual addition was defined as cigarettes plus hookah usage. Two trained and calibrated dental hygienists conducted all examinations. Visual and tactile intraoral examinations were conducted, followed by VELscope® Vx florescence examinations. All subjects received an inspection of the lips, labial mucosa, buccal mucosa, floor of …

Rnai Therapeutics In Autoimmune Disease, Kaleb M. Pauley, Seunghee Cha

Science and Mathematics Faculty Publications

Since the discovery of RNA interference (RNAi), excitement has grown over its potential therapeutic uses. Targeting RNAi pathways provides a powerful tool to change biological processes post-transcriptionally in various health conditions such as cancer or autoimmune diseases. Optimum design of shRNA, siRNA, and miRNA enhances stability and specificity of RNAi-based approaches whereas it has to reduce or prevent undesirable immune responses or off-target effects. Recent advances in understanding pathogenesis of autoimmune diseases have allowed application of these tools in vitro as well as in vivo with some degree of success. Further research on the design and delivery of effectors of …