Oral Biology and Oral Pathology Commons^™

Critical-Size Defects Reconstruction With Four Different Bone Grafts Associated With E-Ptfe Membrane: A Histomorphometric Experimental In Vivo Study, Gustavo Fernandes, Filipe Castro, Rafael Martins Pereira, Wendel Teixeira, Sergio Gehrke, Julio Cesar Joly, Juan Blanco Carrion, Juliana Campos Hasse Fernandes

MOSDOH Faculty Publications

Objectives: The goal of this study was to assess the newly formed bone and the remnant biomaterial by comparing four different bone grafts used to treat critical-size defects, associated or not with the non-resorbable membrane.

Materials and methods: Two calvaria critical-size bone defects were created in 50 male Wistar rats. They were divided into blood (G1), autogenous (G2), bioglass (G3), hydroxyapatite (G4), and xenograft (G5) groups, associated or not with e-PTFE. The experimental periods were 15 and 45 days. Sections were prepared for histomorphometric assessment. All data were analyzed by the mixed-effects model with multiple comparisons (significance level, p < .05).

Results: …

Oral Focal Mucinosis: A Case Report, Hala El-Kammar Dr, Yara Youssef, Bassant Mahmoud Moustafa, Iman M. Mostafa, Noha Adel Hassanine, Adham Fahmy, Rami Guiha Dr.

Future Dental Journal

Oral focal mucinosis is a rare lesion with less than 60 cases reported in English literature so far, to the best of our knowledge. It is considered to be the oral counterpart of cutaneous focal mucinosis and/or cutaneous myxoid cyst. Histopathologically, Oral focal mucinosis is demonsterates a well-circumscribed area of myxomatous connective tissue containing mucinous material, surrounded by denser collagenous connective tissue. We present a case of Oral focal mucinosis occurring on the buccal gingiva of the lower jaw.

Oral Manifestations Of Metastatic Diseases: A Clinical And Histologic Analysis Of 7 Cases, Zeming Zheng Dds, Elizabeth Philipone Dmd

The New York State Dental Journal

Metastatic diseases comprise 1-3% of malignant oral neoplasms with nearly one-third of patients being unaware of having a primary cancer1-4,9. This retrospective analysis identified all oral lesions diagnosed as metastatic disease in the CDM Department of Oral Pathology’s oral biopsy database from 2015-2020 and reported on patient demographics, clinical presentation and diagnosis, origin of the primary, and whether the patient had known history of the disease. From 17,500 total cases, 7 cases were identified as metastatic disease and described in detail. Clinicians’ recognition and awareness of these lesions’ often evasive, ambiguous oral presentations may help facilitate diagnosis, treatment, and prognosis.

Sars-Cov-2 Infectivity, And Interaction With Ace2 In The Human Oral Cavity: A Comprehensive Study, Blake Joseph Henkel

Undergraduate Honors Theses

The oral cavity has the ability to transport infections throughout the body and be infected by bacteria, fungi, protozoa, and viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the virus that led to the most recent pandemic. SARS-CoV-2 has unique ways of interacting with the human body to infect individuals, which has led researchers and scientists to have limited knowledge on its long-term impact on human health. However, there are ways to identify various oral cavity infections and prevent infection now and in the future. Many studies have been conducted in an attempt to understand the body’s …

The Role Of Hdac6 In Periodontal Inflammation, Hannah S. Lohner

Theses and Dissertations

Periodontal disease is a polymicrobial infection-induced chronic inflammatory disease and results in irreversible tissue damage and bone loss. While bacteria play a large role in the initiation of this disease, excessive and prolonged inflammatory immune responses are fundamental for its progression, making host directed therapy (HDT) a possible and potentially valuable treatment. Histone Deacetylase 6 (HDAC6) is an enzyme that removes acetyl groups from both histone and non-histone proteins, giving it a potential role in regulating inflammation initiated by pathogenic oral bacteria. Previous studies indicated that HDACs act as a bivalent regulator in inflammatory responses; however, the role that HDAC6 …

Histological Evaluation Of Pulp Response To Pulpine Ne Versus Biodentine As Direct Pulp Capping Materials In A Dog Model, Hagar Bastawy, Maha Ahmed Niazy, Mona Farid, Asmaa Harhash, Mona Aboelwafa, Ashraf Abu-Seida

International Arab Journal of Dentistry

Introduction: The material used for pulp capping has a significant impact on the outcome of vital pulp therapy. This study compared the pulp tissue response to Pulpine NE versus Biodentine as direct pulp capping materials in a dog model.

Methods: Twenty-four teeth in two mongrel dogs (1-2-year-old) were used. In each dog (n=12 teeth), the dental pulps were exposed in 8 teeth (2 experimental groups, 4 teeth each) and left unexposed in 4 teeth (control group, n=4 teeth). A class V cavity was performed on the buccal surface of the selected teeth in the experimental groups. The exposed …

การเพาะเลี้ยงเซลล์ 3 มิติด้วยแม่เหล็กของเซลล์ต้นกำเนิดจากเซลล์เนื้อเยื่อในฟันมนุษย์และศักยภาพในการนำไปใช้ในทันตกรรมฟื้นฟู, นิก้า จอร์เจีย ซาลาซ่าร์

Chulalongkorn University Theses and Dissertations (Chula ETD)

การศึกษานี้พัฒนาระบบไฮโดรเจลอัลจิเนตที่ถูกออกซิไดซ์ เพื่อส่งผ่านปัจจัยกระตุ้นการเจริญของเซลล์ประสาททั้งแบบต่อเนื่องและทางอ้อม เพื่อส่งเสริมการเปลี่ยนแปลงไปเป็นเซลล์ประสาทของสเต็มเซลล์ที่ได้จากจากเนื้อเยื่อในฟันของมนุษย์ โดยได้ทำการวิเคราะห์ลักษณะของไฮโดรเจลในด้านระดับการออกซิไดซ์ การดูดซับน้ำ การย่อยสลาย และการปลดปล่อยโปรตีน ผลแสดงให้เห็นถึงการปลดปล่อยโปรตีนต้นแบบและ ปัจจัยกระตุ้นการเจริญของเซลล์ประสาทได้ตามช่วงเวลา ในการเพาะเลี้ยงเซลล์แบบ สองมิติ การร่วมเลี้ยงแบบทางอ้อมกับไฮโดรเจลที่พัฒนา พบว่าการแสดงออกของยีนและโปรตีนของตัวบ่งชี้เซลล์ประสาท (เนสติน, ดีซีเอกซ์และ เบต้าทรี ทุบูลิน) เพิ่มขึ้นอย่างมีนัยสำคัญ ความมีชีวิตของเซลล์ยังคงสูงตลอดการทดลอง แสดงถึงความเข้ากันได้ทางชีวภาพของไฮโดรเจลนี้ สำหรับการเปลี่ยนแปลงเป็นเซลล์ประสาทในระบบ สามมิติ ได้มีการสร้างสเฟียร์รอยด์ที่มีชีวิตและมีโครงสร้างแน่นหนาสำเร็จด้วยเทคนิค การปรินต์สามมิติด้วยแม่เหล็กผลการทดลองพบว่าสเฟียร์รอยด์ที่ใช้จำนวน10,000 เซลล์จะมีรูปร่างและความมีชีวิตที่เหมาะสมที่สุด นอกจากนี้ผลการทดลองปัจจุบันแสดงให้เห็นว่ามีศักยภาพของไฮโดรเจลในฐานะเป็นแพลตฟอร์มในการส่งผ่านโปรตีนเพื่อกระตุ้นการพัฬนาไปเป็นเซลล์ประสาทโดย ไม่ต้องใช้โครงร่างและสนับสนุนการประยุกต์ใช้งานในอนาคตกับงานวิจัยสเต็มเซลล์ระดับสามมิติและในระดับคลินิกในเชิงเวชศาสตร์ฟื้นฟูในสาขาทันตกรรมเอนโดดอนต์

Biological Effects Of Photobiomodulation On C2c12 Cell Culture Under Inflammatory Environment, Duong Le Dai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Backgrounds: In the last decade, photobiomodulation (PBM) has been utilized as a complementary therapeutic option for the management of musculoskeletal disorders such as temporomandibular disorders (TMD). However, its mechanism of action in skeletal muscle cells like the C2C12 myoblasts remains poorly understood. Thus, this study aimed to investigate the effects of PBM, using low-level light therapy (LLLT), on the proliferation and differentiation capacity of C2C12 cells cultured under normal media and compared with cells under proinflammatory environments. Materials and Methods: PBM treatment consisted of the following LLLT dose regimens: 1-day of 3, 5, and 7 J/cm2/day single doses and 2-day …

Studying The Relationship Between Polyherbal Mouthwash And Oral Microbiome, Waris Wattanawongwan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: This study aimed to comparatively evaluate the effects of polyherbal, chlorhexidine (CHX), and normal saline (NCC) mouthwashes on oral microbiome composition, microbial metabolic pathways, and patient-reported outcomes, exploring polyherbal alternatives in response to CHX-associated dysbiosis. Methods: A randomized, double-blind trial was conducted among 27 gingivitis patients, randomly assigned to polyherbal, CHX, or NCC mouthwash groups. Participants used the mouthwash twice daily for 7 days. Saliva samples were analyzed via 16S rRNA sequencing. Microbial diversity (alpha and beta diversity) and composition were statistically evaluated. Differential taxa and pathways were identified using FDR-corrected Wilcoxon tests and log₂ fold-change analysis. Patient-reported outcomes …

The Impact Of Gestational Diabetes Mellitus And Maternal Oral Microbiome: A Scoping Review, Jaclyn P. Langan

Honors Undergraduate Theses

Background: Gestational diabetes mellitus (GDM) is a common pregnancy-related metabolic disorder associated with adverse maternal and fetal outcomes. Recent research has highlighted the potential role of the oral microbiome in the pathophysiology of various systemic conditions, including diabetes mellitus. However, the impact of GDM on the maternal oral microbiome remains relatively understudied. Understanding alterations in the oral microbiome during pregnancy complicated by GDM could provide valuable insights into the mechanistic links between systemic metabolic disorders and oral health.

Objective: The objective of this scoping review was to comprehensively examine the existing literature on the relationship between GDM and maternal oral …

Digital Assessment Of Soft And Hard Tissue Alterations After Anterior Maxillary Tooth Extraction, Robin Jalkh, Stephanie Mrad, Emile Chrabieh, Joe Bassil

International Arab Journal of Dentistry

Objectives: This study aimed to assess dimensional alterations in soft and hard tissues 12 weeks post-extraction using novel computerized methods. Secondary objectives included exploring potential correlations between soft tissue changes and underlying bone variations, as well as investigating relationships between soft and hard tissue alterations and various contributing factors.

Methods: For all patients, preoperative and postoperative CBCT scans and intra-oral scans were obtained. Soft and hard tissue dimensions before extraction and at 12 weeks post-extraction were measured using 3D-slicer software. Both DICOM and STL files were superimposed, and measurements were taken at 2mm and 4mm from the facial …

Ai And Radiologist Assessments On The Prevalence Of Tmj Osteoarthritis Using Radiographic Images: A Comparative Study, Louloua Mourad, Nayer Abo El Saad, Yehia El Mahallawy

International Arab Journal of Dentistry

Objectives: This study aims to address the significant discomfort and functional impairment associated with temporomandibular joint osteoarthritis (TMJ OA), which negatively impacts the quality of life. It emphasizes the importance of prompt diagnosis and explores the potential of an Artificial Intelligence (AI) system to enhance TMJ OA diagnosis.

Methods: The prevalence of TMJ OA was evaluated using 3 diagnostic tools: the gold standard, the AI model, and an examiner. In total, 132 patients who performed 190 cone-beam computed tomography (CBCT) images were included.

Results: The prevalence of TMJ OA was 62.11% using the gold standard, 63.68% using …

Assessment And Quantification Of The Microbial Flora In The Output Water Of Two Types Of Dental Chairs: A Comparative Study, Khalil Kharma, Louis Hardan, Cynthia Kassis, Rim Bourgi, Regina Geitani, May Mallah, Elie Maalouf, Carina Mehanna Zogheib

International Arab Journal of Dentistry

Objectives: Dental Unit Waterlines (DUWL) have shown to be a perfect host for different pathogenic microorganisms. The purpose of this study was to analyze and compare the level of bacterial contamination in the output water of 2 types of DUWL.

Methods: Dental unit water samples from the air/water syringe of the A-dec (A-dec™ Performer 200, Newberg, USA) DUWL type and KaVo (ESTETICA™ E30/E70/E80 Vision, Kavo, Biberach, Germany) type were collected and analyzed for total aerobic flora, Pseudomonas aeruginosa, faecal coliforms, total coliforms, faecal streptococci and sulfite-reducing anaerobic flora.

Results: The bacteriological analysis of water samples shows the …

Pdgfra And B Copositive Fibroblasts Drive Fibrosis In Mouse Salivary Glands Through Tgfβ Signaling, Renae Williams Atkinson

Electronic Theses & Dissertations (2024 - present)

Fibrosis is of significant concern to the medical community as numerous disease processes are characterized by progressive fibrosis leading to organ damage. We begin the process of examining the mechanism of fibrosis to salivary gland hypofunction and briefly consider Sjögren’s Disease (SjD). Method: We employed single-cell RNA sequencing data from a reversible mouse salivary gland injury model and from NOD/ShiLtJ mice, a model of secondary SjD. We performed treatment comparisons with the help of Seurat dotplots and UMAPS. Using differential gene expression analysis and the publicly available R packages: clusterProfiler, WikiPathways and Cytoscape, we identified the processes and pathways …

Effect Of Triple Antibiotic Paste, Double Antibiotic Paste, And Calcium Hydroxide On Antibiotic Resistance Of Tet Repressor Proteins, Tetracycline Resistance Gene W, And Tetracycline Resistance Gene Q: A Randomized Controlled Clinical Trial, Ertuğrul Karataş, Özgen Kırmızıbekmez, Mustafa Özkan Baltacı, Ahmet Adıgüzel

Journal of Dentistry Indonesia

Objective: To investigate the effect of the use of Calcium Hydroxide (Ca(OH)2), Triple Antibiotic Paste (TAP), and Double Antibiotic Paste (DAP) as intra-canal medicaments on the development of antibiotic resistance by evaluating tetracycline genes. Methods: Forty five patients were randomly assigned into 3 groups according to the intracanal medication using a web program (www.randomizer.org), (Ca(OH)2, TAP and DAP). Root canal bacteriological samples were collected before root canal treatment (S1) and after chemo-mechanical procedures (S2). Following root canal shaping procedures, intra-canal medication of the root canals was performed with selected intra-canal medicament. A second appointment was planned for the …

The Effect Of Several Electric Cigarette Puffs On Nanohybrid Composite Resin Surface Roughness, Alvi Rizqi Amaliyah, Haris Budi Widodo, Irfan Dwiandhono

Journal of Dentistry Indonesia

Nanohybrid composite resin is a dental restorative material comprising micro and nano-sized fillers. When accompanied by smoking habits, it can alter the surface roughness of composite resin. Objective: This study aimed to determine the effect of several electric cigarette (e-cigarette) puffs on the surface roughness of nanohybrid composite resin. Methods: This study was conducted in the experimental laboratory with a pretest-posttest control group design using 48 nanohybrid composite resin specimens divided into six groups. Subsequently, the experimental groups were exposed to 75, 150, 225, 300, and 450 puffs of e-cigarette, and the control group was given artificial saliva …

Management Of Ankyloglossia In A Non-Syndromic Patient Using A Super Pulsed Laser: A Life-Changing Treatment- A Case Report, Urfi Jawed, Afaf Zia, Ramniwas Kumawat, Afshan Bey, Maahin Mahmood

Journal of Dentistry Indonesia

Objective: Ankyloglossia affects a person’s overall well-being and life, from malnutrition due to poor feeding and mechanical problems to psychological stress and low self-esteem, thus affecting social life. This case reports the use of non-scalpel techniques, such as a super pulsed laser, to manage ankyloglossia in a non-syndromic patient suffering from speech incompetence and restricted tongue mobility and highlights its advantages over other conventional techniques. Case Report: A 17-year-old male patient presented with a complaint of restricted tongue mobility, due to which he could not pronounce certain words clearly. As the patient was apprehensive about the use of …

Contents Vol 30 No 3 2023, Dr. Yuniardini Septorini Septorini Wimardhani

Journal of Dentistry Indonesia

This is the content of Vol. 30 number 3 2023.

Case Report And X-Ray Microtomography And Scanning Electron Microscopy Analysis Of Teeth With Hypocalcified Amelogenesis Imperfecta, Rodrigo W. Marinoni, Thiago G. Silva, Leonardo S. Benato, Anelize M B Rumbelsperger, Antonio Adilson Soares De Lima

Journal of Dentistry Indonesia

Amelogenesis imperfecta (AI) is a genetic disease characterized by producing morphological and structural changes in tooth enamel. Objective: To highlight the morphological changes observed in teeth with hypocalcified AI based on scanning electron microscopy and microtomography analysis. Case report: An adult male had teeth seriously compromised by hypocalcified AI. The treatment plan involved removal of third molars followed by restorative and prosthetic treatment. After extraction, the teeth were analyzed by SEM and X-ray microtomography (microCT). Teeth with AI revealed the presence of microfractures arranged from the outer surface to the center of the enamel. Microfractures had a cracked or “cracked …

Comparison And Assessment Of Design, Handling Characteristics, And Efficacy Of Hand Instruments In Calculus Removal, Mohan Kumar Pasupuleti, Krishna Ganesh, Gautami S Penmetsa, Boddeda Anusha, Haripriya Narukurthi, Kommina Mouna Sahith

Journal of Dentistry Indonesia

Objective: primary objective of periodontal therapy is to remove supra and subgingival deposits from the tooth surface to stop disease progression. The purpose of the study is to address the instrument selection and its efficacy in calculus removal. Methods: A total of 50 subjects who fulfilled the criteria were included in the study. Participants were subjected to a complete periodontal examination, and the responses from 50 dental house surgeons were compared based on the instrument design, handling characteristics, and efficacy of calculus removal by using two sets of instruments. Results: The LM instrument was rated as best …