Oral Biology and Oral Pathology Commons^™

Case Report And X-Ray Microtomography And Scanning Electron Microscopy Analysis Of Teeth With Hypocalcified Amelogenesis Imperfecta, Rodrigo W. Marinoni, Thiago G. Silva, Leonardo S. Benato, Anelize M B Rumbelsperger, Antonio Adilson Soares De Lima

Journal of Dentistry Indonesia

Amelogenesis imperfecta (AI) is a genetic disease characterized by producing morphological and structural changes in tooth enamel. Objective: To highlight the morphological changes observed in teeth with hypocalcified AI based on scanning electron microscopy and microtomography analysis. Case report: An adult male had teeth seriously compromised by hypocalcified AI. The treatment plan involved removal of third molars followed by restorative and prosthetic treatment. After extraction, the teeth were analyzed by SEM and X-ray microtomography (microCT). Teeth with AI revealed the presence of microfractures arranged from the outer surface to the center of the enamel. Microfractures had a cracked or “cracked …

Comparison And Assessment Of Design, Handling Characteristics, And Efficacy Of Hand Instruments In Calculus Removal, Mohan Kumar Pasupuleti, Krishna Ganesh, Gautami S Penmetsa, Boddeda Anusha, Haripriya Narukurthi, Kommina Mouna Sahith

Journal of Dentistry Indonesia

Objective: primary objective of periodontal therapy is to remove supra and subgingival deposits from the tooth surface to stop disease progression. The purpose of the study is to address the instrument selection and its efficacy in calculus removal. Methods: A total of 50 subjects who fulfilled the criteria were included in the study. Participants were subjected to a complete periodontal examination, and the responses from 50 dental house surgeons were compared based on the instrument design, handling characteristics, and efficacy of calculus removal by using two sets of instruments. Results: The LM instrument was rated as best …

Elastic Recovery Properties Of Alginate Impression Materials Based On Red Algae Kappaphycus Alvarezii, Didin Erma Indahyani, Izzata Barid, Nafisah Nafisah, Depi Praharani, Agus Sumono, Niken Probosari, Sulistiyani Sulistiyani

Journal of Dentistry Indonesia

Alginate impression materials in dentistry are used to form details of the oral cavity organs. The alginate content in the impression material functions to form a gel. One source of alginate is algae. Objective: This study aims to determine the recovery elasticity and setting time of alginate impression materials made from red algae K. alvarezii. Methods: The sodium alginate in the composition of this alginate impression material was extracted from red algae K. alvarezii. The alginate impression material was tested for setting time and recovery elasticity and compared with commercial impression material (Hygedent®). Results: The elastic …

Enigmatic Cervicofacial Actinomycosis Post Radiotherapy - A Case Report, Amanda Phoon Nguyen

Journal of Dentistry Indonesia

Although thought to be uncommon, it is believed that cervicofacial actinomycosis is underreported due to frequent misdiagnosis. It has been called the “most misdiagnosed disease by experienced clinicians”. A wide range of dental procedures or trauma, including tooth eruption, may precipitate actinomycosis by enabling microorganisms to penetrate the deeper tissue layers via an entry point. This infective disease is significant due to its potentially aggressive and locally destructive nature. This case describes a patient diagnosed with cervicofacial actinomycosis. Her history prior to this was significant for proliferative verrucous leukoplakia, and T4aN0M0 (AJCC 7th Edition) squamous cell carcinoma of the right …

Clinically Effective Regenerative Therapy For Primary Endodontic Lesions With Secondary Periodontal Lesions: A Case Report, Martina Amalia, Budi Budi, Iceu Estu Kurmaena, Nevi Yanti, Hesty Nurcahyanti, Aini Hariyani Nasution

Journal of Dentistry Indonesia

The diagnosis and prognosis of teeth with endo-perio lesions present a major challenge for dentists. Proper diagnosis is critical in determining treatment and the long-term prognosis. However, treating complex endodontic and periodontal lesions is one of the most common challenges in current clinical practice. The coexistence of pulpal and periodontal tissue damage may complicate diagnosis and further influence dental prognosis. This article consists of a case report evaluating the efficacy of xenografts and resorbable membrane pericardium in treating bifurcation lesions associated with periodontal lesions of the left mandibular first molar. A 22-year-old female patient was referred by conservative dentistry with …

Genetically Engineering Gingipains For High Yield Large Scale Purification., Andrew E. Fuchs

Electronic Theses and Dissertations

Porphyromonas gingivalis is a keystone oral pathogen in the progression of periodontal disease and has been implicated in the etiology of many other systemic human diseases. P. gingivalis produces primary virulence factors termed the gingipains, a group of cysteine proteases. There is a high demand for high purity gingipains for downstream in vitro studies where P.g. is implicated, both oral and systemic. Gingipain production candidate mutant P.g. strains were generated to isolate each gingipain for large scale purification and the purified gingipains characterized. Two mutants were determined not to be viable for large scale purification of gingipains, each for different …

Verruciform Xanthoma, Khanh Trinh Dmd, Daniel Nassimi Dds, Daria Vaislyeva Dds, Scott Peters Dds

The New York State Dental Journal

Verruciform xanthoma (VX) is a benign epithelial proliferation typically found on the masticatory mucosa. VX usually affects patients in their 5 th to 6 th decade of life. VX is treated with simple excision with a low chance of recurrence after surgery.

Adenoid Ameloblastoma With Dentinoid, Eric Silver Dmd, Md, Stephen Roth Dds, Lydia Lam Dds, John Fantasia Dds, Steve Yusupov Dds, Md

The New York State Dental Journal

Adenoid ameloblastoma with dentinoid (AAD) is a rare benign neoplasm of odontogenic epithelium, with under 50 cases having been reported in the literature. AAD shares histopathological characteristics with both ameloblastomas and adenomatoid odontogenic tumors. They are unique because of dentinoid deposition without enamel. We present a case of AAD in the maxilla of a 13 year old. The patient was treated with left infrastructure maxillectomy and left partial infratemporal fossa dissection. This case reports on an extremely rare disease process in an uncommon location in a pediatric patient.

N Y State Dent J November 2023

The New York State Dental Journal

In the November 2023 issue, the reader will find the following feature articles:

• Verruciform Xanthoma
• Use of a 3D Intraoral Scanner for Prosthetic Rehabilitation of Cocaine-Induced Oronasal Fistula
• Adenoid Ameloblastoma with Dentinoid

This issue includes regular columns with regional news impacting the New York membership including: editorial and perspectives columns, legal, association activities, component news, continuing education opportunities, and classifieds.

Cost-Effectiveness Of [99mtc]Tilmanocept Relative To [99mtc]Sulfur Colloid For Sentinel Lymph Node Biopsy In Early Stage Oral Cavity Cancer, Karen Y Choi, Qiang Hao, Kathryn Carlisle, Christopher S Hollenbeak, Stephen Y Lai

Faculty, Staff and Student Publications

BACKGROUND: Several studies have demonstrated varying rates of efficacy, reliability, and sensitivity of sentinel lymph node biopsy (SLNB) in identifying occult nodal disease for early stage oral cavity squamous cell carcinoma (OCSCC) depending on the radionuclide agent utilized. No head-to-head comparison of cost or clinical outcomes of SLNB when utilizing [99mTc]tilmanocept versus [99mTc]sulfur colloid has been performed. The goal of this study was to develop a decision model to compare the cost-effectiveness of [99mTc]tilmanocept versus [99mTc]sulfur colloid in early stage OCSCC.

PATIENTS AND METHODS: A decision model of disease and treatment as a function of SLNB was created. Patients with …

Natural Remedies To Combat Aberrant Hallmark Signatures Including Altered Glycosylation In Oral Carcinoma, Kruti A. Mehta, Jayendra B. Patel, Prabhudas S. Patel

Research Symposium

Background: Tobacco associated oral cancers remain a major concern in India with higher incidence and mortality making it an Indian-centric burning issue. To combat this dreadful disease, we investigated effects of certain natural compounds on the hallmark signatures including glycosylation transcripts levels in oral carcinoma.

Methods: The tongue carcinoma cells- SAS cells were treated with tobacco compounds, natural compounds and Cisplatin. RNA was isolated from the cells and converted to cDNA. RT-qPCR was performed to evaluate expression levels of various genes.

Results: The treatment of tobacco compounds resulted in similar pattern of altered makers (ST3GAL1, NEU3, FUT5, FUT6, MMP2, BCL2) …

Candida-Induced Granulocytic Myeloid-Derived Suppressor Cells Are Protective Against Polymicrobial Sepsis, Shannon Esher Righi, Amanda J. Harriett, Elizabeth A. Lilly, Paul L. Fidel Jr., Mairi C. Noverr

School of Dentistry Faculty Publications

Polymicrobial intra-abdominal infections (IAI) can lead to life-threatening sepsis with significant morbidity and mortality, especially when pathogenic fungi are involved. We have employed an established clinically relevant mouse model of fungal/bacterial IAI and shown that immunization with low-virulence Candida species, that is, Candida dubliniensis, can induce responses that protect against sepsis via the suppression of lethal inflammation. This protection is dependent on long-lived Gr-1(+) polymorphonuclear leukocytes that display characteristics consistent with myeloid-derived suppressor cells (MDSCs) and trained innate immunity. Here we aimed to functionally and phenotypically characterize these protective Gr-1(+) leukocytes. Compared to nonimmunized control mice, we observed increased levels …

Spatially-Aware Clustering Improves Ajcc-8 Risk Stratification Performance In Oropharyngeal Carcinomas, Guadalupe Canahuate, Andrew Wentzel, Abdallah S R Mohamed, Lisanne V Van Dijk, David M Vock, Baher Elgohari, Hesham Elhalawani, Clifton D Fuller, G Elisabeta Marai

Faculty, Staff and Student Publications

OBJECTIVE: Evaluate the effectiveness of machine learning tools that incorporate spatial information such as disease location and lymph node metastatic patterns-of-spread, for prediction of survival and toxicity in HPV+ oropharyngeal cancer (OPC).

MATERIALS & METHODS: 675 HPV+ OPC patients that were treated at MD Anderson Cancer Center between 2005 and 2013 with curative intent IMRT were retrospectively collected under IRB approval. Risk stratifications incorporating patient radiometric data and lymph node metastasis patterns via an anatomically-adjacent representation with hierarchical clustering were identified. These clusterings were combined into a 3-level patient stratification and included along with other known clinical features in a …

The Biocompatibility Of A Ginger-Containing Herbal Toothpaste On Developing Zebrafish Embryos, Simge Meseli, Unsal Veli Ustundag, Perihan Seda Ates, Ismail Unal, Ebru Isik Alturfan, Dilek Tağtekin, Funda Yanikoglu

Journal of Dentistry Indonesia

The biocompatibility of toothpaste in an oral cavity should be approved by clinical trials. Nowadays, herbal toothpaste is increasing in popularity due to its natural ingredients. Being genetically similar to humans, zebrafish are used in potential toxicity testing. The zebrafish embryotoxicity test is a fast and straightforward method to study chemical toxicity during embryogenesis.

Objective: This study aimed to evaluate if there was any biocompatibility of the toothpaste on zebrafish embryos.

Methods: Adult AB strain zebrafish were used according to Institutional Animal Care and Use Committee protocols. Normally dividing, spherical embryos were exposed to herbal toothpaste with ginger (Gumgumix, Turkey) …

Oral Leukoplakia: Diagnosis And Management Revisited, Isaäc Van Der Waal

Journal of Dentistry Indonesia

The definition of oral leukoplakia has not much changed during the past five decades and is still a definition by exclusion of ‘known’ lesions. Therefore, a diagnosis of leukoplakia is not always a straightforward one for the clinicians and, to some extent, also for the pathologists. The traditional clinical classification in homogeneous and nonhomogeneous leukoplakia may just be simplified into leukoplakia (thin and thick/verrucous) and erythroleukoplakia. In spite of numerous reported predictive molecular and genetic parameters of malignant transformation, the presence and grade of epithelial dysplasia as assessed by histopathological examination is still the most important one. Of the various …

Salivary Changes In Patients Hospitalized For Complications Of Hiv Infection/Aids: A Study Using Inductively Coupled Plasma Optical Emission Spectrometry, Vanessa Cavassin Klamas, Mateus Anhaia Ventura, Loana Mara Baika, Adryano Arana Kamei, Jhonatan Yukio Naka, Cassiano Lima Chaiben, Marco Tadeu Grassi, Antonio Adilson Soares De Lima

Journal of Dentistry Indonesia

Objective: The aim of this study was to analyze the salivary flow and levels of major salivary ions of HIV-infected individuals. Methods: One hundred and two participants (18 - 74 years of age) were divided into two groups (51 HIV - infected patients and 51 controls) and had a saliva sample collected. Salivary flow rate was measured gravimetrically. Levels of salivary sodium, potassium, magnesium, calcium and phosphorus were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). Results: The mean salivary flow of subjects with HIV infection was significantly higher when compared to controls (Case group = …

Perceived Stress, Severity Of Xerostomia, And Periodontal Status In Undergraduate Dental Students, Engku Ahmad Muzhaffar, Zurairah Berahim, Norsila Abdul Wahab, Norkhafizah Saddki

Journal of Dentistry Indonesia

Dentistry is a highly stressful program; stress is associated with xerostomia and periodontal disease.

Objective: This study aims to investigate the association of perceived stress, severity of xerostomia, and periodontal status in dental students.

Methods: This was a two-phase cross-sectional study of 245 Universiti Sains Malaysia (USM) undergraduate dental students. Phase 1 involved administration of the Perceived Stress Scale (PSS-10) and Summated Xerostomia Inventory (SXI). In phase 2, the Community Periodontal Index (CPI) was performed on 150 students to determine their periodontal status.

Results: Mean PSS-10 and SXI scores were 19.6 (SD 5.47) and 7.9 (SD 2.04), respectively. …

How To Manage Fan’S C Shape Canals Along With S Shape Morphology In Indian Population: A Case Series And Narrative Review, A Lavanya, Rajendra Kumar Tewari

Journal of Dentistry Indonesia

The C-shaped root canals offer greater negotiation, debridement, and obturation difficulty as they present with intercommunications, lateral canals, and reduced dentin thickness.

Objective: This case series elaborates on the recognition that these varying configurations is important to enhance adequate cleaning, shaping, and sealing of the root canal.

Case Reports: On clinical examination, all the teeth showed caries were nonresponsive to sensibility tests and toother diagnostic tests, suggestive of pulp necrosis with apical periodontitis. Conventional root canal treatment with different techniques and obturation systems have been used. This case series presents the successful management of various C-shaped configurations classified by Fan …

Association Of Inflammatory Markers And Growth Factors With Radiographically Assessed Wound Healing Of Extraction Sockets, Sasha Mohammadi

Theses & Dissertations

The purpose of this prospective cohort pilot study was to investigate the association of the periodontal status and inflammatory markers from the gingival crevicular and wound fluid with the healing outcomes following tooth extraction. The central research hypothesis is that extraction sites and the adjacent teeth that exhibit increased markers of inflammation, or decreased growth factors, at baseline or 2 weeks will be associated with reduced healing outcomes observed radiographically 3 months following surgery. Nine patients at the UNMC College of Dentistry who were seeking care to extract a posterior tooth due to advanced periodontal bone loss, non-restorable caries or …

Expression Of Vimentin, Α-Sma And Tgf-Β In Different Grades Of Oral Squamous Cell Carcinoma, Hala El-Kammar, Waleed F. Esmael, Nermeen S. Afifi

Future Dental Journal

Abstract

Background: Oral Squamous cell carcinoma (OSCC) is typically discovered at an early stage; nevertheless, regional lymph node metastases are prevalent; therefore, surgical excision of the primary tumor is commonly coupled with neck dissection and radiotherapy. Cancer cells become invasive by acquiring a mesenchymal phenotype, a process known as the epithelial-to-mesenchymal transition. Vimentin, α-SMA and TGF-β expressions are believed to take part in EMT and tumor progression. Aim: this work aimed to evaluate the expression of Vimentin, α-SMA and TGF-β on OSCC progression and hence propose potential role as potential prognostic markers or therapeutic targets. Materials and methods: twenty-four specimens …