Oral Biology and Oral Pathology Commons^™

C7: Comparison Of Four Dental Pulp-Capping Agents By Cone-Beam Computed Tomography And Histological Techniques—A Split-Mouth Design Ex Vivo Study, Muruganandhan J, Sujatha G

Annual Research Symposium

Dental pulp-capping is done to preserve vital teeth when the pulp is exposed due to caries, trauma or instrumentation. Various materials are used as pulp-capping agents. The introduction of newer materials requires scientific studies to assess their clinical efficacy. The study was designed as a split-mouth randomized analysis of four pulp-capping agents (calcium hydroxide, mineral trioxide aggregate (MTA), Biodentine and EndoSequence root repair material (ERRM)). Based on selection criteria, 15 orthodontic patients requiring the extraction of four premolars (60 teeth total) were included in the study. After pulp-capping, the teeth were extracted after 8 weeks. We analyzed the extracted teeth …

Histological And Ultrastructural Study Of Silver Nanoparticles Toxicity And The Possible Protective Effect Of Vitamin C On Submandibular Salivary Glands Of Albino Rats, Mohamed Shamel, Dalia Riad, Mahmoud Al Ankily

Dentistry

Introduction: Despite silver nanoparticles (AgNPs) beneficial effect in many fields, it has been shown that AgNPs can induce toxic effects. Cytotoxic effect of AgNPs is related to the increased release of reactive oxygen species (ROS) which may lead to cell death. Natural antioxidant scavengers like vitamin C have the capacity to bind and neutralize ROS thus having a vital role in cellular defense against oxidative stress. Objective: The objective of the current study was to assess the cytotoxic impact of AgNPs on the submandibular salivary glands of Albino rats and to reveal the potential antioxidant influence of vitamin C to …

Streptococcus Mutans And The Oral Microbiome, Danielle Pintone

Honors Program Contracts

No abstract provided.

Histological And Ultrastructural Study Of Silver Nanoparticles Toxicity And The Possible Protective Effect Of Vitamin C On Submandibular Salivary Glands Of Albino Rats, Mohamed Shamel, Mahmoud Ankily

Dentistry

Introduction: Despite silver nanoparticles (AgNPs) beneficial effect in many fields, it has been shown that AgNPs can induce toxic effects. Cytotoxic effect of AgNPs is related to the increased release of reactive oxygen species (ROS) which may lead to cell death. Natural antioxidant scavengers like vitamin C have the capacity to bind and neutralize ROS thus having a vital role in cellular defense against oxidative stress. Objective: The objective of the current study was to assess the cytotoxic impact of AgNPs on the submandibular salivary glands of Albino rats and to reveal the potential antioxidant influence of vitamin C to …

Immediate And Early Loading Of Hydrothermally Treated, Hydroxyapatite-Coated Dental Implants: A 7-Year Prospective Randomized Clinical Study, Afarin Arghami, David Simmons, Jeanne St Germain, Pooja Maney

School of Dentistry Faculty Publications

Background Existing research on marginal bone stability around hydroxyapatite (HA)-coated implants often lacks adequate long-term follow-up. The purpose of this randomized prospective study was to evaluate the 7-year outcome of patients with immediate and early loaded single-tooth restorations supported by implants with plasma-sprayed, partially HA-coated surfaces. Forty-two patients in need of 50 single implants were treated in in the Postgraduate Periodontics Clinic of Louisiana State University School of Dentistry. Implants were randomly divided into 2 groups: Group A was immediately loaded, and Group B was early loaded. Continuous follow-up with periodic maintenance care and radiographic evaluations was performed. The primary …

The Potential Of Raman Spectroscopy In The Diagnosis Of Dysplastic And Malignant Oral Lesions, Ola Ibrahim, M. Toner, Steven Flint, Hugh Byrne, Fiona Lyng

Articles

Early diagnosis, treatment and/or surveillance of oral premalignant lesions are important in preventing progression to oral squamous cell carcinoma (OSCC). The current gold standard is through histopathological diagnosis, which is limited by inter and intra observer and sampling errors. The objective of this work was to use Raman spectroscopy to discriminate between benign, mild, moderate and severe dysplasia and OSCC in formalin fixed paraffin preserved (FFPP) tissues. The study included 72 different pathologies from which 17 were benign lesions, 20 mildly dysplastic, 20 moderately dysplastic, 10 severely dysplastic and 5 invasive OSCC. The glass substrate and paraffin wax background were …

Biomedical Applications Of Vibrational Spectroscopy: Oral Cancer Diagnostics, Hugh Byrne, Isha Behl, Genecy Calado, Ola Ibrahim, M. Toner, Sheila Galvin, Claire M. Healy, Steven Flint, Fiona Lyng

Articles

Vibrational spectroscopy, based on either infrared absorption or Raman scattering, has attracted increasing attention for biomedical applications. Proof of concept explorations for diagnosis of oral potentially malignant disorders and cancer are reviewed, and recent advances critically appraised. Specific examples of applications of Raman microspectroscopy for analysis of histological, cytological and saliva samples are presented for illustrative purposes, and the future prospects, ultimately for routine, chairside in vivo screening are discussed.

The Influence Of Infant Formula On The Growth Of Commensal And Pathogenic Streptococcus Species In The Infant Oral Cavity, Geneva Waynick

Undergraduate Honors Theses

The oral microbiome is a complex community of microorganisms that can both reflect and greatly influence the health of the human host. A number of diseases are associated with dysbiotic oral microflora in infants and children, including dental (e.g. dental caries, gingivitis, and periodontal disease), and gastrointestinal diseases (e.g. pediatric appendicitis, celiac disease, and pediatric inflammatory bowel disease). A variety of factors can influence the composition of the oral microbial community in infants, including gestation length, mode of delivery, feeding method, and diet. This study focuses on the effects of diet on the growth of a commensal bacterium (Streptococcus …

Establishing The Minimally Important Clinical Difference For The Oral Health Impact Profile In Malaysians Provided With Removable Partial Dentures, Siti Fauzza Ahmad

Student Works (2020-2029)

Future demand for a removable partial denture (RPD) remains as more adults retain their natural teeth into old age. Partial tooth loss often leads to masticatory function disturbances and negatively affects the quality of life (QoL). Prosthodontic rehabilitation with RPD improves QoL; however, the statistically significant change with many Oral Health-Related Quality of Life (OHRQOL) instruments, may not be clinically meaningful. The Malaysian version of Oral Health Impact Profile, OHIP(M)-45 questionnaires has been validated and adapted; however, its clinical interpretability has not been established. The smallest change in OHRQoL score perceived by a patient as beneficial, following an intervention, is …

The Effect Of Egf On Vegf Expression On Submandibular Salivary Gland Of Albino Rats Receiving Doxorubicin, Mohamed Shamel, Mohamed Mansy, Rabab Mubarak

Dentistry

Background: Vascular endothelial growth factor (VEGF) has two prominent biological effects of inducing mitogenesis and migration of vascular endothelial cells and to produce permeability on the microvasculature. Epidermal growth factor (EGF), known by its mitogenic activity on different types of cells, could induce the proliferation and differentiation of the epithelial cells and stimulate its regeneration. Doxorubicin (DXR) is a widely used anticancer drug and is one of most commonly used chemotherapeutic drugs, however it may cause some serious complications such as toxicity in various tissues in the body. Aims: The present study aims to investigate the effect of EGF on …

S. Gordonii-Produced Hydrogen Peroxide Modulates Mir-663a And Ccl20 Expression In Oral Epithelial Cells, Marshall Houston Maynard

Theses and Dissertations--Medical Sciences

The mechanisms through which a persistent recognition of commensal bacteria by oral epithelial cells (OECs) mitigates an uncontrolled inflammatory response of the oral mucosa remain unknown. CCL20 secretion by OECs in response to pathogenic bacteria is regulated by S. gordonii (Sg)-induced miR-663a; nevertheless, the mechanisms involved in these Sg-modulated responses remain to be elucidated. Since Sg is a hydrogen peroxide (H₂O₂) producer, and H₂O₂ has been shown to stimulate miRNA expression, we hypothesized that H₂O₂ could be involved in Sg-induced miR663a and CCL20 responses. Expression of miR663a …

Evaluation Of Anti-Cancer Effect Of Different Concentrations Of Black Raspberries Extract On Oral Squamous Cell Carcinoma Cell Line (In Vitro Study), Sara Abdelwahed

Dentistry

Background: Black Raspberry has a wide range of pharmacological and therapeutic aspects including anti-inflammatory and antioxidant activities. Moreover, the effect of Black Raspberry has been studied in many cancers such as human cervical, esophageal and colorectal cancer and showed cytotoxic and anti-proliferative effects against these types of cancer. Aim: To identify the possible anti-cancer effect of Black Raspberry on Oral Squamous Cell Carcinoma cell line. Material and methods: Human tongue squamous cell carcinoma cell line was divided into 3 groups: one control group, second group treated with Black Raspberry, and the third group treated with Doxorubicin, each of the two …

Systemic Versus Local Injection Of Bone Marrow Mesenchymal Stem Cells On 5-Fluorouracil Treated Parotid Glands Of Albino Rats, Caroline Maged Massieh, Medhat El Zainy, Reham Magdy Amin, Iman Fathy

Dentistry

ABSTRACT Objective: Head and neck cancer is the sixth leading cancer by incidence worldwide and eighth by death. Recent reports revealed that, not only radiotherapy but also chemotherapy may induce xerostomia. The aim of this study was to compare the possible regenerative effect of BMSCs through systemic and local injections. Material and Methods: 52 male Albino rats were randomly divided into 4 groups: Group 1: 10 rats received 0.5 ml of PBS by injection. Group 2: 14 rats received an intraperitoneal injection of 5-FU drug. Group 3: 14 rats were injected the same dose of 5-FU then received an intraglandular …

Oral Mucormycosis In Post-Covid- 19 Patients: A Case Series, Enji Ahmed Mahmoud, Asmaa Abobakr, Radwa Raghib, Ayman El-Gawish, Dalia Galwash

Dentistry

No abstract provided.

Comparisons Of Oral, Intestinal, And Pancreatic Bacterial Microbiomes In Patients With Pancreatic Cancer And Other Gastrointestinal Diseases, Mei Chung, Naisi Zhao, Richard Meier, Devin C. Koestler, Guojun Wu, Erika De Castillo, Bruce J. Paster, Kevin Charpentier, Jacques Izard, Karl T. Kelsey, Dominique S. Michaud

Department of Food Science and Technology: Faculty Publications

Background: Oral microbiota is believed to play important roles in systemic diseases, including cancer. Methods: We collected oral samples (tongue, buccal, supragingival, and saliva) and pancreatic tissue or intestinal samples from 52 subjects, and characterized 16S rRNA genes using high-throughput DNA sequencing.

Results: Bray–Curtis plot showed clear separations between bacterial communities in the oral cavity and those in intestinal and pancreatic tissue samples. PERMANOVA tests indicated that bacterial communities from buccal samples were similar to supragingival and saliva samples, and pancreatic duct samples were similar to pancreatic tumor samples, but all other samples were significantly different from each …

Chitosan/Biphasic Calcium Phosphate Composite Scaffold Incorporated With Trichostatin A For Bone Regeneration, Teerawat Sukpaita

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study synthesized a chitosan-based scaffold incorporated with trichostatin A (TSA), an epigenetic modifier molecule, using a freeze-drying technique to achieve promising bone regeneration potential. The scaffolds with various biphasic calcium phosphate (BCP) proportions: 0%, 10%, 20%, and 40% were characterized. The addition of BCP improved the scaffolds' mechanical properties and delayed the degradation rate. From all physicochemical parameters, 20% BCP scaffold matched the appropriate bone scaffold requirements and was selected for further development. The proper concentration of TSA was also tested. Our developed scaffold released TSA and sustained them for up to 3 days. The scaffold with 800 nM …

Role Of Extracellular Adenosine Triphosphate (Eatp) On Immunomodulatory Function Of Human Periodontal Ligament Cells, Maythwe Kyawsoewin

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Adenosine triphosphate, a nucleotide that acts as an important extracellular messenger, is released into the extracellular environment during various physiologic and pathological conditions. Human periodontal ligament cells (hPDLCs) can release adenosine triphosphate into the extracellular environment in response to mechanical stress. Extracellular adenosine triphosphate (eATP) plays role in both inflammation and osteogenic differentiation processes. eATP also participates in immunosuppressive action on immune cells. However, the role of eATP on the immunomodulatory function of hPDLCs is still unclear. This study aims to investigate the effects of eATP on the immunomodulatory function of hPDLCs and the participation of specific purinergic P2 …

Anti-Inflammatory And Regeneration Properties Of Iloprost On Inflamed Human Dental Pulp Cells Model: An In Vitro Study, Rahman Wahyudi

Chulalongkorn University Theses and Dissertations (Chula ETD)

In the clinical situation, pulp exposure can happen during caries removal procedures. Inflammation can occur on the pulp of the exposed area because of infiltration of bacteria from caries or as a result of pulp reaction to caries removal drilling. When the pulp was exposed during the caries removal procedure, traditional methods to preserve the pulp such as pulp capping and pulpotomy were performed. The ideal treatment outcome of the pulp exposure is to regain the primary structure of tubular dentin as well as maintain the vitality and healthiness of the dental pulp Thus, good dental materials or drugs used …

Effect Of Shear Force On Immunosuppressive Property Of Human Periodontal Ligament Cells, Ravipha Suwittayarak

Chulalongkorn University Theses and Dissertations (Chula ETD)

The immunomodulatory property is to regulate immune cell function. IDO and TGF-β1 inhibited T cell proliferation and induced regulatory T (Treg) cells differentiation. However, this property must be activated by inflammation. Recently, mechanical forces have also activated this property. Hence, this study investigated effect of shear stress on immunosuppressive property of hPDL cells. Cells were subjected to shear stress then expression of IDO, TGF-β1, IFN-gamma and COX2 mRNA and protein was examined by qRT-PCR, ELISA, and IDO activity. Conditioned media derived shear stress were treated with CD4+ T cells. The proliferative T cell effect was determined using resazurin. The FOXP3, …

Effects Of Small Molecules In Wnt Signaling On Human Dental Pulp Cells, Chatvadee Kornsuthisopon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Osteoblast differentiation requires interaction of several cell signaling to coordinately modulate cell responses. Understanding the integration between signaling pathways provides an insight into molecular mechanism that governs stem cell differentiation. Notch, Wnt, and Interleukin15 (IL15) signaling are among the crucial pathways that control biological processes including osteogenic differentiation. Therefore, this dissertation was focused on modulating these signaling pathways in human dental pulp stem cells (hDPSCs). First, the roles of Wnt signaling in dental pulp homeostasis and dentin regeneration were reviewed. Previous evidence suggested that Wnt might be a promising target cell signaling for dentin regeneration. Thus, effect of Tideglusib, a …