Oral Biology and Oral Pathology Commons^™

Selected Transcriptome Profiles Of Oral Cancer Suggestive Of Field Cancerisation Using Second Generation Sequencing, Rosnah Binti Zain, Anwar A., Karen-Ng L. P., Cheong S. C., Zaini Z., Prepagaran N., Hercus R., Idris N. M., Saidin A., Chia J. Y., Merican A. F., Abraham M. T., Tay K. K., Mustaffa W. M. W., Norain A. T., Rahman Z. A. A., Jallaludin A.

Prof. Dr. Rosnah Binti Zain

Objectives: To characterize differential gene expression between oral cancerous tissues and the normal mucosal tissues of oral cancer patients and oral mucosal tissues of cancer free patients using second generation sequencing. Methods: Five fresh frozen oral cancer tissue samples from smoking patients, with 2 samples of proximal normal were included in this study. An additional 8 samples consisting of normal oral mucosal tissues from the alveolar mucosa of non-cancer patients who had their wisdom tooth removed where 4 were smokers and another 4 were non-smokers was also included. The tissues and socio-demographic information were obtained from the Malaysian Oral Cancer …

Oral Mucositis: The New Paradigms, Rajesh V. Lalla, Douglas E. Peterson

UCHC Articles - Research

Purpose of review

Mucositis has long been viewed as an unavoidable consequence of high-dose chemotherapy and/or radiation. Management has been directed to supportive care including oral pain control, nutritional support, infection treatment and control of diarrhea. While these interventions have been valuable for clinical management, they have not been collectively directed to molecularly targeted prevention and treatment. This review addresses recent advances regarding mucosal injury in cancer patients, with emphasis on symptom clusters, genetically-based tissue susceptibility and risk prediction, imaging technology, and computational biology.

Recent findings

Modeling of symptom clusters in cancer patients continues to mature. Although integration of mucositis …

Transcriptional Profiling Of Oral Squamous Cell Carcinoma Using Formalin-Fixed Paraffin-Embedded Samples, Saleh A., Rosnah Binti Zain, Hussaini H., Ng F., Tanavde V., Hamid S., Chow A. T., Lim G. S., Abraham M. T., Teo S. H., Cheong S. C.

Prof. Dr. Rosnah Binti Zain

Despite the advances in cancer treatment, the 5-year survival rate for oral cancer has not changed significantly for the past 40 years and still remains among the worst of all anatomic sites. Gene expression microarrays have been used successfully in the identification of genetic alterations in cancer development, however, these have hitherto been limited by the need for specimens with good quality intact RNA. Here, we demonstrated the use of formalin-fixed paraffin-embedded tissues in microarray experiments to identify genes differentially expressed between cancerous and normal oral tissues. Forty-three tissue samples were macrodissected and gene expression analyses were conducted using the …

A Bootstrap-Anfis Framework For Oral Cancer Prognosis Based On Clinical And Genomic Markers, Chang S. W., Sameem A. K., Rosnah Binti Zain, Merican A. F. M. A.

Prof. Dr. Rosnah Binti Zain

The incidence of oral cancer is high in Malaysia especially for those of Indian ethnic origin. In this research, an oral cancer prognosis prediction model will be developed by using Bootstrap-ANFIS techniques. The aim of this research is to improve the prediction of oral cancer survival and to identify the key clinical and genomic markers for the survival prediction of oral cancer. The proposed model is designed to handle small medical dataset with limitations in terms of time, cost and tissue samples.

Cytotoxicity Of Polyurethane Dimethacrylate Derived From Palm Oil Polyol., Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

Objective: The aim of this study was to investigate the cytotoxicity effect of polyurethane dimethacrylate monomer derived from palm oil polyol (PUDMA) and 2 experimental composite resins based on these monomer PUDMA- based composites) compared to an experimental Bis-GMA/TEGDMA-based composite and EsthetX flowable composite (Dentsply, Caulk, USA). Methods: The experimental composite resins were prepared by mixing 0.25% and 0.75% by weight camphorquinone and ethyl (4-dimethyl amino) benzoate with 60% filler for each of the following monomer systems; I) PUDMA monomer (PUDMA-based composite resin), II) Bis-GMA/TEGDMA at ratio of 75:25 by weight, III) PUDMA/BisGMA-TEGDMA at ratio of 50:50 by weight. Eight …

Surface Reproduction Of Elastomeric Materials: Viscosity And Groove Shape Effects, Noor Hayaty Abu Kasim Abu Kasim N.H.

Noor Hayaty Abu Kasim Abu Kasim N.H.

Objective: To evaluate the effect of viscosity and type of grooves on surface detail reproduction of elastomeric impression materials. Methods: Express putty/light-, Impregum medium- and heavy/light-bodied and Aquasil medium- and putty/light-bodied elastomeric impression materials were chosen for this study. Five impressions were made using a cylindrical aluminum reference block with U- and V- shaped grooves and to produce 35 master dies. Each master die was immersed in distilled water at 370 C for 5 minutes prior to the impression making on moist surfaces. Surface topography of the dies and impressions were captured using Alicona Imaging System. The mean difference in …

Inherent Differential Propensity Of Dental Pulp Stem Cells Derived From Human Deciduous And Permanent Teeth, Govindasamy V., Abdullah A. N., Ronald V. S., Musa S., Aziz Z. A. C. A., Rosnah Binti Zain, Totey S., Bhonde R. R., Kasim N. H. A.

Prof. Dr. Rosnah Binti Zain

Introduction: Lately, several new stem cell sources and their effective isolation have been reported that claim to have potential for therapeutic applications. However, it is not yet clear which type of stem cell sources are most potent and best for targeted therapy. Lack of understanding of nature of these cells and their lineagespecific propensity might hinder their full potential. Therefore, understanding the gene expression profile that indicates their lineage-specific proclivity is fundamental to the development of successful cell-based therapies. Methods: We compared proliferation rate, gene expression profile, and lineage-specific propensity of stem cells derived from human deciduous (SCD) and permanent …