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Functionalizing Nanoparticles With Cafa Protein To Target Bar Peptide For Oral Delivery Applications., Hetal Desai 2020 University of Louisville

Functionalizing Nanoparticles With Cafa Protein To Target Bar Peptide For Oral Delivery Applications., Hetal Desai

Electronic Theses and Dissertations

Background: Porphyromonas gingivalis adherence to Streptococcus gordonii may be important for P. gingivalis colonization in the oral cavity. Nanoparticles encapsulating synthetic peptide BAR (BAR-NPs) inhibit P. gingivalis adherence more potently than free BAR. However, BAR-NPs would exhibit low retention in an open flow environment. Hypothesis: Targeting BAR-NPs to the streptococcal surface using CafA protein will enhance their efficacy. Methods: CafA-modified NPs encapsulating BAR were synthesized using double emulsion approach. Surface binding and retention, and release kinetics of BAR from CafA-modified NPs was assessed. Functional inhibition assays were performed using dual a species biofilm. Results: CafA-modified NPs demonstrated specificity of adhesion, …


The Emerging Oral Pathogen, Filifactor Alocis, Disrupts Neutrophil Functions To Enhance Survival And Dysregulate Inflammation., Irina Miralda Molina 2020 University of Louisville

The Emerging Oral Pathogen, Filifactor Alocis, Disrupts Neutrophil Functions To Enhance Survival And Dysregulate Inflammation., Irina Miralda Molina

Electronic Theses and Dissertations

Periodontitis is an irreversible, chronic inflammatory disease where pathogenic microbial communities accumulate in the gingival crevice. Advances in culture-independent techniques have facilitated the identification of new bacterial species in periodontal lesions, such as the Gram-positive anaerobe, Filifactor alocis. Neutrophils are a major component of the innate host response, and the outcome of their interaction with F. alocis may be a determinant of oral health status. While neutrophil functions typically protect the host against periodontal disease, oral pathogens have adapted to evade or disarm neutrophil microbicidal functions while promoting mechanisms that drive inflammation, which also provides a source of nutrients …


Health-Related Anxiety In The Management Of Oral Soreness In An Hivseropositive Patient, Masita Mandasari, Zulvia Oktanida Syarif, Febrina Rahmayanti 2020 Oral Medicine Residency Program, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

Health-Related Anxiety In The Management Of Oral Soreness In An Hivseropositive Patient, Masita Mandasari, Zulvia Oktanida Syarif, Febrina Rahmayanti

Journal of Dentistry Indonesia

The number of people living with HIV (PLHIV) has been increasing since the first known case in the early 1980s. However, PLHIV can also experience comorbidities, such as health anxiety. In the oral cavity, anxiety is often associated with the etiology of parafunctional habits. Anxiety can be measured using self-administered instruments, such as the Hospital Anxiety and Depression Scale (HADS) and Short-Health Anxiety Inventory (SHAI), which is specifically used in the medical setting. Objectives: We are describing a case of HIV positive patient with health anxiety who presented to the oral medicine clinic in our hospital with a complaint of …


Differential Diagnosis And Histopathological Analysis Of Localized Gingival Overgrowths: Study Of 218 Cases From Northeast Thailand, Lordjie Marr Morilla, Ajiravudh Subarnbhesaj, Poramaporn Klanrit 2020 Department of Clinical Dental Health Sciences, College of Dentistry, University of the Philippines, Manila

Differential Diagnosis And Histopathological Analysis Of Localized Gingival Overgrowths: Study Of 218 Cases From Northeast Thailand, Lordjie Marr Morilla, Ajiravudh Subarnbhesaj, Poramaporn Klanrit

Journal of Dentistry Indonesia

Localized gingival overgrowths (LGOs) are relatively common clinical findings. These lesions can be associated with chronic irritation, infections, or they can represent benign or malignant tumors that can either be primary or metastatic. The etiology of these lesions is multifactorial, but the most prevalent cause is reactive hyperplasia and inflammation induced by dental plaque. Objective: This study presents the prevalence of LGOs in Northeast Thailand and identifies the most common LGOs in the region. Methods: A series of 218 LGO cases was studied. Results: Pyogenic granuloma (PG), gingival squamous cell carcinoma (GSCC), and peripheral ossifying fibroma (POF) were the three …


Evaluation Of Clinical Single Dental Implants Made Between 2013 And 2017, Zozan Erdoğmuş 2020 Ministry of Health, Diyarbakır Oral and Dental Health Hospital, Diyarbakır, Turkey

Evaluation Of Clinical Single Dental Implants Made Between 2013 And 2017, Zozan Erdoğmuş

Journal of Dentistry Indonesia

Objective: This retrospective study aimed to determine the profile of patients who underwent single tooth implants between 2013 and 1017 and to evaluate long-term follow-up and success. Methods: The results of 79 patients who underwent single dental intra-bone dental implantation in Diyarbakır Oral and Dental Health Hospital between 2013 and 2017 were evaluated separately in terms of gender, age, implanted area and number, systemic diseases affecting implant health, and missing implants. Results: In this study, single tooth implants were evaluated in 79 patients with a mean age of 39.55 years in men and 30.44 years in women. …


Erupted Compound Odontoma Associated With Missing Tooth: A Rare Case Report, Weeraya Tantanapornkul, Ruchadaporn Kaomongkolgit, Sirilawan Tohnak, Lalitkorn Promma, Soranan Chantarangsu 2020 Department of Oral Diagnosis, Faculty of Dentistry, Naresiun University, Phitnasulok 65000, Thailand

Erupted Compound Odontoma Associated With Missing Tooth: A Rare Case Report, Weeraya Tantanapornkul, Ruchadaporn Kaomongkolgit, Sirilawan Tohnak, Lalitkorn Promma, Soranan Chantarangsu

Journal of Dentistry Indonesia

Odontomas are one of the most frequently found odontogenic tumors that consist of dental tissues. Odontomas have been classified into two types: compound and complex odontomas. They are usually discovered on routine radiographic examination. In rare cases, the odontoma erupt into the oral cavity, called an erupted odontoma. The first case was published in 1980, and since then, there have been not more than 50 cases being reported in the literature. Most of the reported cases are erupted complex odontoma. This case report represents a rare case of an erupted compound odontoma in a young patient who came with the …


The Effect Of Egf On The Ultrastructure Of Submandibular Salivary Glands Of Albino Rats Receiving Doxorubicin, Mohamed Shamel, Mohamed Mansy, Rabab Mubarak 2020 The British University in Egypt

The Effect Of Egf On The Ultrastructure Of Submandibular Salivary Glands Of Albino Rats Receiving Doxorubicin, Mohamed Shamel, Mohamed Mansy, Rabab Mubarak

Dentistry

Objectives: The present study was designed to detect the outcome of EGF injection on the cytotoxic effect of DXR induced in submandibular SG of rats regarding its ultrastructure. Methods: 30 male Albino rats, two months old and 200gm body weight were divided into three groups. Group I assisted as a control group. Group II (DXRgp), 20 mg/kg body weight DXR was injected intraperitoneally into the rats. Whereas group III (DXR+ EGF) the rats received the same dose of DXR and on the next day, they were injected intraperitoneally with EGF in a daily dose of 10µg/kg bodyweight for 7 consecutive …


Synthesis And Characterization Of Hemocompatible Antibiotic Nanoparticles, Elvin Muhindo Walemba 2020 Loma Linda University

Synthesis And Characterization Of Hemocompatible Antibiotic Nanoparticles, Elvin Muhindo Walemba

Loma Linda University Electronic Theses, Dissertations & Projects

Porphyromonas gingivalis is a causative agent of periodontal disease and a risk factor for cardiovascular disease. Novel treatment of periodontal disease using nanomaterials can supplement or replace the use of antibiotics and mechanical debridement. Nanomaterials can be optimized for effectiveness and specificity and minimum toxicity. Synthesized Ag nanoparticles stabilized in Pluronic F127 were used to produce bimetallic core/shell Ag/Au nanoparticles with optimized Ag+ release and reduced toxicity. We tested the F127-stabilized nanoparticles for their antimicrobial activity and hemocompatibility in planktonic bacteria and citrated whole blood, respectively. Both Ag and Ag/Au bimetallic nanoparticles inhibited P. gingivalis W83 growth. We then evaluated …


Relationship Between Oral Health And Clinical Osteoporosis Among Hospitalized Patients With And Without Diabetes, Kenneth Izoura, Gayle Allenback, Amber Champion, Civon Gewelber, Michael Neubauer 2020 University of Nevada, Las Vegas

Relationship Between Oral Health And Clinical Osteoporosis Among Hospitalized Patients With And Without Diabetes, Kenneth Izoura, Gayle Allenback, Amber Champion, Civon Gewelber, Michael Neubauer

School of Medicine Faculty Research

Objective: Diabetes mellitus (DM) is associated with poor oral health and osteoporosis (OP). The aim of this study was to assess the relationship between OP, periodontal disease (PD), and other dental and health outcomes in a cohort of hospitalized patients with and without DM. Method: Using a cross-sectional study design, we enrolled consecutive hospitalized patients. We administered a questionnaire to gather demographic information, oral health history, smoking history, and history of OP. We inspected their dentition and reviewed their charts. Data were analyzed using t-tests, chi-square tests, and logistic regression models. Result: Out of 301 patients enrolled, 275 had PD, …


The Effects Of Vaping On Oral Streptococci And Oral Inflammation, Matthew Caldwell 2020 University of Central Florida

The Effects Of Vaping On Oral Streptococci And Oral Inflammation, Matthew Caldwell

Honors Undergraduate Theses

E-cigarette (e-cig) use is rising, but much is unknown about the effects of its vapor. This vapor contains chemicals such as propylene glycol, a known antimicrobial, and nicotine, whose derivatives are carcinogenic. Here, we study the effects of vaping on resident bacteria of the oral cavity and on oral cell inflammation. Oral streptococci are major residents in the oral cavity, with S. mutans the primary cause of dental caries. Growth and biofilm formation have been shown to be enhanced upon exposure to traditional cigarette smoke in vitro. In this study, we analyzed the effects of e-cig vapor on growth …


The Role Of Manganese In Streptococcus Sanguinis, Tanya M. Puccio 2020 Virginia Commonwealth University

The Role Of Manganese In Streptococcus Sanguinis, Tanya M. Puccio

Theses and Dissertations

Streptococcus sanguinis is primarily associated with oral health as a commensal bacterium. As an opportunistic pathogen, S. sanguinis is capable of colonizing heart valve vegetations, leading to the disease infective endocarditis. Previous studies from our lab have identified the high-affinity manganese transporter SsaACB as important for endocarditis virulence. The impact that manganese depletion has on S. sanguinis had never been evaluated and a secondary manganese transporter has not been identified. Thus, we employed the use of a fermentor to control large-scale growth over time and depleted manganese in an ΔssaACB mutant using a metal chelator, EDTA. The changes in …


Raman Microspectroscopic Study For The Detection Of Oral Field Cancerisation Using Brush Biopsy Samples, Isha Behl, Genecy Calado, Anika Vishwakarma, Stephen Flint, Sheila Galvin, Claire Healy, Marina Leite Pimentel, Alison Malkin, Hugh Byrne, Fiona Lyng 2020 Technological University Dublin

Raman Microspectroscopic Study For The Detection Of Oral Field Cancerisation Using Brush Biopsy Samples, Isha Behl, Genecy Calado, Anika Vishwakarma, Stephen Flint, Sheila Galvin, Claire Healy, Marina Leite Pimentel, Alison Malkin, Hugh Byrne, Fiona Lyng

Articles

Field cancerisation (FC) is potentially an underlying cause of poor treatment outcomes of oral squamous cell carcinoma (OSCC). To explore the phenomenon using Raman microspectroscopy, brush biopsies from the buccal mucosa, tongue, gingiva and alveolus of healthy donors (n = 40) and from potentially malignant lesions (PML) of Dysplasia Clinic patients (n = 40) were examined. Contralateral normal samples (n = 38) were also collected from the patients. Raman spectra were acquired from the nucleus and cytoplasm of each cell, and subjected to partial least squares-discriminant analysis (PLS-DA). High discriminatory accuracy for donor and PML samples was achieved for both …


Effect Of Different Scaling Methods And Materials On The Enamel Surface Topography: An In Vitro Sem Study, Mahmoud Al Ankily, Fatma Makkeyah, Mohamed Shamel, Mahmoud Bakr 2020 The British University in Egypt

Effect Of Different Scaling Methods And Materials On The Enamel Surface Topography: An In Vitro Sem Study, Mahmoud Al Ankily, Fatma Makkeyah, Mohamed Shamel, Mahmoud Bakr

Dentistry

Aims and Objectives: Scaling is important for maintenance of gingival and periodontal conditions. These procedures have a harmful effect on the dental hard tissues. The aim of this study was to investigate the effects of hand and ultrasonic instruments made of stainless and titanium on the surface properties of enamel. Materials and Methods: Forty extracted premolars were used in this in vitro study and were randomly divided into four groups (n = 10). Group I received ultrasonic scaling with stainless steel tip, group II received ultrasonic scaling with titanium tip, group III hand scaling with stainless steel tip, and group …


Effects Of Aflatoxin B1 On The Submandibular Salivary Gland Of Albino Rats And Possible Therapeutic Potential Of Rosmarinus Officinalis: A Light And Electron Microscopic Study, Mohamed Shamel, Bassant ashraf 2020 The British University in Egypt

Effects Of Aflatoxin B1 On The Submandibular Salivary Gland Of Albino Rats And Possible Therapeutic Potential Of Rosmarinus Officinalis: A Light And Electron Microscopic Study, Mohamed Shamel, Bassant Ashraf

Dentistry

Background: Aflatoxin B1 (AFB1), a highly toxic mycotoxin, is one of the contaminants of food items such as corn, rice, nuts, and flour. This study aimed to evaluate the effect of AFB1 on the histology and ultrastructure of the submandibular salivary glands (SMSG) of albino rats and examine the possible therapeutic effect of Rosmarinus officinalis extract. Methods: This study used 21 adult male albino rats equally divided into three groups as follows: Group C (saline-treated control group); Group A (AFB1 treated group) subjected to intraperitoneal injection of AFB1 (2 mg/kg) once daily for four weeks; Group R …


Effect Of Different Scaling Methods And Materials On The Enamel Surface Topography: An In Vitro Sem Study, Mohamed Shamel, Mahmoud Bakr, Mahmoud Ankily, Fatma Makkeyah 2020 The British University in Egypt

Effect Of Different Scaling Methods And Materials On The Enamel Surface Topography: An In Vitro Sem Study, Mohamed Shamel, Mahmoud Bakr, Mahmoud Ankily, Fatma Makkeyah

Dentistry

Aims and Objectives: Scaling is important for maintenance of gingival and periodontal conditions. These procedures have a harmful effect on the dental hard tissues. The aim of this study was to investigate the effects of hand and ultrasonic instruments made of stainless and titanium on the surface properties of enamel. Materials and Methods: Forty extracted premolars were used in this in vitro study and were randomly divided into four groups (n= 10). Group I received ultrasonic scaling with stainless steel tip, group II received ultrasonic scaling with titanium tip, group III hand scaling with stainless steel tip, and group IV …


The Effect Of Smoking On Clinical Presentation And Expression Of Tlr-2 And Cd34 In Oral Lichen Planus Patients: Clinical And Immunohistochemical Study, Enji Ahmed Mahmoud, Nermin Yousif, Nermin Raouf 2020 The British University in Egypt

The Effect Of Smoking On Clinical Presentation And Expression Of Tlr-2 And Cd34 In Oral Lichen Planus Patients: Clinical And Immunohistochemical Study, Enji Ahmed Mahmoud, Nermin Yousif, Nermin Raouf

Dentistry

Background: Oral lichen planus is a chronic inflammatory disease which is considered as a potential precancerous condition. Numerous studies have confirmed that inflammation is a strong risk factor for cancer development. Smoking is associated with potentially malignant disorders of the oral and oropharyngeal mucosa. The adverse consequences of smoking in various pathologies are mediated by its effects on the immune-inflammatory system. Little is known about the influence of cigarette smoke content on the course of OLP and inflammatory response. Methods: Twenty oral lichen planus smoker patients, 20 oral lichen planus non-smoker patients and 20 control patients were included in this …


Selenomonas Sputigena: Growth Conditions And Projected Virulence Factors Of An Emerging Oral Pathogen, Kayla A. Keller 2020 Virginia Commonwealth University

Selenomonas Sputigena: Growth Conditions And Projected Virulence Factors Of An Emerging Oral Pathogen, Kayla A. Keller

Theses and Dissertations

Periodontal diseases are ubiquitous diseases. They can affect people through a chronic infection that happens over a lifetime, but also as an aggressive infection that afflicts the younger population. It not only results in tooth loss and a lower quality of life, but it can also lead to fatal secondary infections like cervical cancer, heart disease, and Alzheimer’s disease. This study looks at Selenomonas sputigena, an emerging oral pathogen, that has been hypothesized to contribute to periodontal disease. It has been linked to fatal septicemia and has been found in increased abundance within the oral biofilm during peak infection. …


Mageb2 Antibody As Potential Diagnostic And Predictive Tool In The Progression Of Oral Cancer, Awang Hasyim Noratikah 2020 Universiti Malaya

Mageb2 Antibody As Potential Diagnostic And Predictive Tool In The Progression Of Oral Cancer, Awang Hasyim Noratikah

Student Works (2020-2029)

Introduction: Oral squamous cell carcinoma (OSCC) represents more than 90% of all oral cancer. Despite advances in diagnostic and therapeutic modalities, mortality and morbidity rates have not improved in the last decade. Hence, alternative diagnostic and therapeutic approaches are urgently needed. Cancer testes antigens (CTA) are proteins that are expressed in various malignant tumours but its expression in normal tissue is restricted to testes and occasionally placental trophoblast. Due to its exclusive presence in different types of malignant tumors, and their ability to trigger immune responses, CTA are considered promising biomarkers for cancer vaccines. A previous protein array study reported …


Differential Expression Of Mucin In Salivary Gland Tumours, Mahamad Apandi Nurul Inaas 2020 Universiti Malaya

Differential Expression Of Mucin In Salivary Gland Tumours, Mahamad Apandi Nurul Inaas

Student Works (2020-2029)

Introduction: Varied alterations in types of secreted mucin may affect the regulation of cell growth, immune response and adhesion of cell. These changes may indirectly contribute to the ability of tumour invasion and metastasis. However, the expression of mucins in salivary gland tumours has not been explored in depth. Objectives: To investigate expressions of mucin in the salivary gland tumour microenvironment and make comparisons between benign and malignant, and minor and major salivary gland tumours. Methods: Special stains were used to stain neutral mucin (Periodic acid Schiff), sialomucin (Alcian Blue) and sulfomucin (Aldehyde Fuschin) within tissues from 6 normal salivary …


Investigation Of Inflammatory Responses Following The Use Of Novel Anisotropic Hydrogel Tissue Expander In The Skin Of Maxillofacial Region, Salim Igdayir Kholoud Mohamed Ali 2020 Universiti Malaya

Investigation Of Inflammatory Responses Following The Use Of Novel Anisotropic Hydrogel Tissue Expander In The Skin Of Maxillofacial Region, Salim Igdayir Kholoud Mohamed Ali

Student Works (2020-2029)

Background: The purpose of tissue expander is to grow healthy supplementary tissue under the controlled of mechanical force exerted by the tissue expander. In the field of reconstructive and plastic surgery, the use of soft tissue expanders are important for growing healthy supplementary tissue. However, the molecular mechanism associated with the inflammatory response associated with the force generated from the use of tissue expanders is still not characterized. Among the many types of tissue expanders, anisotropic type of hydrogel tissue expander was chosen due to its ability to produce controlled incremental inflation on the tissue that enhance cellular adaptation and …


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