Epigenomic Dysregulation-Mediated Alterations Of Key Biological Pathways And Tumor Immune Evasion Are Hallmarks Of Gingivo-Buccal Oral Cancer, 2019 National Institute of Biomedical Genomics
Epigenomic Dysregulation-Mediated Alterations Of Key Biological Pathways And Tumor Immune Evasion Are Hallmarks Of Gingivo-Buccal Oral Cancer, Debodipta Das, Sahana Ghosh, Arindam Maitra, Nidhan K. Biswas, Chinmay K. Panda, Bidyut Roy, Rajiv Sarin, Partha P. Majumder
Journal Articles
Background: Gingivo-buccal oral squamous cell carcinoma (OSCC-GB) is the most common cancer among men in India and is associated with high mortality. Although OSCC-GB is known to be quite different from tongue cancer in its genomic presentation and its clinical behavior, it is treated identically as tongue cancer. Predictive markers of prognosis and therapy that are specific to OSCC-GB are, therefore, required. Although genomic drivers of OSCC-GB have been identified by whole exome and whole genome sequencing, no epigenome-wide study has been conducted in OSCC-GB; our study has filled this gap, and has discovered and validated epigenomic hallmarks of gingivobuccal …
Part I: Complete Dentures And Diabetes Correlation, 2019 CUNY New York City College of Technology
Part I: Complete Dentures And Diabetes Correlation, Ibeth Erazo
Publications and Research
Diabetes is one of the most frequent pathologies that dentists encounter, due to its high prevalence worldwide. The aim of this study is to present the correlation between diabetes and complete denture wearers, intending to emphasize the importance of proper control and denture adaptation to oral environment for this type of patient. Within the sphere of the dental office, one of the main complications to consider in this type of patient is periodontal disease, which is their most frequent complication. Other important oral manifestations considered in the research are fungal infections, xerostomia, and burning mouth syndrome. It is common for …
Xerostomia: A Comprehensive Review, 2019 Wayne State University
Xerostomia: A Comprehensive Review, Danevin Putrus
Honors College Theses
This thesis will be highlighting the condition known as Xerostomia. Xerostomia is the condition also known as “dry mouth”. This condition affects many people all throughout the world with symptoms causing bad breath, discomfort, difficulty speaking and many other. The seriousness of this condition can lead to cardiovascular disease, dementia, respiratory infections, diabetes mellitus, rheumatoid arthritis, and even cancer (Absolutedental.com). Currently there is not any one specific solution for the condition although many have been proposed. Throughout the thesis I will closely examine former research studies, current solutions proposed, and propose my own study and solution based on research and …
Differential Microrna Expression Of Mir-21 And Mir-155 With Oral Cancer Extracellular Vesicles In Response To Melationin, 2019 University of Nevada, Las Vegas
Differential Microrna Expression Of Mir-21 And Mir-155 With Oral Cancer Extracellular Vesicles In Response To Melationin, Matthew Hunsaker, Greta Barba, Karl Kingsley, Katherine M. Howard
Dental Medicine Faculty Publications
Objective: Extracellular vesicles derived from oral cancer cells, which include Exosomes and Oncosomes, are membranous vesicles secreted into the surrounding extracellular environment. These extracellular vesicles can regulate and modulate oral squamous cell carcinoma (OSCC) progression through the horizontal transfer of bioactive molecules including proteins, lipids and microRNA (miRNA). The primary objective of this study was to examine the potential to isolate and evaluate extracellular vesicles (including exosomes) from various oral cancer cell lines and to explore potential differences in miRNA content. Methods: The OSCC cell lines SCC9, SCC25 and CAL27 were cultured in DMEM containing 10% exosome-free fetal bovine serum. …
The Q Rule In Bacteriodetes And The Identification And Characterization Of Porphyromonas Gingivalis Glutaminyl Cyclase., 2019 University of Louisville
The Q Rule In Bacteriodetes And The Identification And Characterization Of Porphyromonas Gingivalis Glutaminyl Cyclase., John Andrew Houston
Electronic Theses and Dissertations
Background: Porphyromonas gingivalis, a major pathogen associated with chronic periodontitis, secretes variety of proteins, majority of which begins with glutamine. Several of these proteins were found with pyroglutamate (pGlu) at N-terminus suggesting the presence of this posttranslational modification pathway in P.gingivalis. The observation that N-terminal glutamine is over-represented as the first amino acid after signal peptide cleavage, and subsequent confirmation of pGlu formation on the nascent protein via mass spectrometry, led us to conclude that an enzyme must be present as the executor of this reaction. Hypothesis: PG2157 is a glutaminyl cyclase and is responsible for the cyclization of …
Periodontitis And The Elderly: An Overview Of The Disease And Its Impact On An Aging Population, 2019 Liberty University
Periodontitis And The Elderly: An Overview Of The Disease And Its Impact On An Aging Population, Nicole Grace Weissenfluh
Senior Honors Theses
Periodontitis affects millions of Americans each year, and is especially prevalent among the elderly. Since periodontitis is a chronic, progressive condition, uninterrupted disease progression leads to irreversible oral damage; therefore, periodontitis often reduces the oral health-related quality of life. Furthermore, research strongly suggests a correlation between periodontitis and other systemic diseases (e.g., cardiovascular disease and diabetes mellitus), highlighting the importance of understanding, treating, and preventing periodontitis. This thesis explores the pathology and etiology of periodontitis, with special focus given to the prevalence and impact of the disease in the elderly population. Additionally, the connection between periodontitis and other systemic diseases …
Mass Spectrometry-Based Proteomics Analysis Of Bioactive Proteins In Emd That Modulate Adhesion Of Gingival Fibroblast To Improve Bio-Integration Of Dental Implants, 2019 The University of Western Ontario
Mass Spectrometry-Based Proteomics Analysis Of Bioactive Proteins In Emd That Modulate Adhesion Of Gingival Fibroblast To Improve Bio-Integration Of Dental Implants, David Zuanazzi Machado Jr
Electronic Thesis and Dissertation Repository
Titanium implants are used in dental practice to replace damaged or lost teeth. The implant needs to integrate with the surrounding gingiva to protect it against bacterial invasion that leads to implant loss. The biointegration is dependent on the implant surface that interacts with proteins from biological fluids to modulate tissues response. Tailoring the surface with specific proteins from the enamel matrix derivative (EMD) would be beneficial to improve the implant-gingiva interface since EMD can affect various cells including gingival fibroblasts. A surface-affinity approach using three different titanium surfaces and saliva was utilized as a model in combination with tandem …
Examination Of Strain-Dependent Differences In S. Sanguinis Virulence And Growth, 2019 Virginia Commonwealth University
Examination Of Strain-Dependent Differences In S. Sanguinis Virulence And Growth, Shannon Baker
Theses and Dissertations
Streptococcus sanguinis, an abundant and benign inhabitant of the oral cavity, is an important etiologic agent of infective endocarditis, particularly in people with pre-disposing cardiac valvular damage. Although commonly isolated from patients with IE, little is known about the factors that make any particular S. sanguinis isolate more virulent than another or, indeed, whether significant differences in virulence exist among isolates. To investigate the virulence of multiple isolates, a variation of the Bar-seq (barcode sequencing) method was employed. A conserved chromosomal site was identified for subsequent insertion of a barcode identifier, unique for each strain. Barcode insertion did not …
Put Your Money Where Your Mouth Is: Reforming Organized Dentistry To Address Persistent Oral Health Disparities In The U.S., 2019 Claremont Colleges
Put Your Money Where Your Mouth Is: Reforming Organized Dentistry To Address Persistent Oral Health Disparities In The U.S., Aparna Chintapalli
Pomona Senior Theses
The importance of oral health has been largely neglected from the conventional standards of healthcare in terms of public understanding & prioritization, the industrial infrastructure, and the scope of prevention & early-intervention services. Its adjunctive locus to the field of medicine has lead to the bifurcation of the oral cavity from the rest of the human body. As a result of this divide, there have been multiple factors that have allowed socially stratified oral health outcomes to manifest. This thesis examines the determinants of oral health disparities through a multidisciplinary lens (i.e. biology, public policy, infrastructure), and offers evidence of …
Effect Of Grape Components On Periodontal Disease., 2018 University of Louisville
Effect Of Grape Components On Periodontal Disease., Juili Subodh Shivde
Electronic Theses and Dissertations
Background: Half of the adult American population (47 %) are victims of periodontitis, a chronic inflammatory disease which leads to destruction of the tissues supporting the tooth. Periodontitis is initiated by periodontal pathogens and caused by excessive or prolonged host mediated inflammation. Porphyromonas gingivalisis one important agent implicated in the etiopathogenesis of chronic periodontitis. Pro-inflammatory cytokines induced by periodontal pathogens can cause alveolar bone loss and periodontitis. Bone loss is caused by the imbalance of bone formation and bone resorption. Osteoclasts are major cells that cause bone resorption. Moreover, periodontal inflammation also leads to bone resorption. Grapes are rich …
Influences Of Porphyromonas Gingivalis On The Viability Of Esophageal Cancer Cells., 2018 University of Louisville
Influences Of Porphyromonas Gingivalis On The Viability Of Esophageal Cancer Cells., Muddasir Mohammed
Electronic Theses and Dissertations
A recent study demonstrated an association between the Porphyromonas gingivalis (Pg)infection and the progression of ESCC (Gao et al., 2016). However, how Pg infection affects the nature of ESCC remains unknown. We examined the effects of Pg on the proliferation and chemotherapy drug-induced apoptosis of ESCC cells and elucidated the signaling molecules involved in these processes. Method: Apoptosis and cell proliferation were tested using Flow cytometry and Tetrazolium salt based proliferation assays respectively. Western Blot was utilized to detect the protein expression. CDK2 and Cyclin E were knocked down in the ESCC cells through transfection. Unpaired T-test and …
Synthesis And Characterization Of Targeted Bar Encapsulated Polylactic-Co-Glycolic Acid Nanoparticles To Inhibit Porphyromonas Gingivalis Biofilm Formation., 2018 University of Louisville
Synthesis And Characterization Of Targeted Bar Encapsulated Polylactic-Co-Glycolic Acid Nanoparticles To Inhibit Porphyromonas Gingivalis Biofilm Formation., Ranjith Radha Krishnan
Electronic Theses and Dissertations
Periodontal disease is one of the most prevalent infectious diseases worldwide. Between 30-50% of the global adult population suffers from periodontal disease. Some form of periodontitis is present in 46% of American adults, corresponding to annual expenditures in excess of 14 billion dollars for treatment and prevention. Current treatments for periodontal diseases involve mechanical removal of plaque, correction of risk factors, gingival surgery and/or antibiotic therapy. To our knowledge there is no effective therapeutic approach that aims to limit pathogen colonization of the oral biofilm or re-colonization after treatment. Interaction of the pathogen Porphyromonas gingivalis with oral streptococci is critical …
Green Tea Polyphenol Epigallocatechin-3-Gallate-Stearate Inhibits The Growth Of Streptococcus Mutans: A Promising New Approach In Caries Prevention, 2018 Montclair State University
Green Tea Polyphenol Epigallocatechin-3-Gallate-Stearate Inhibits The Growth Of Streptococcus Mutans: A Promising New Approach In Caries Prevention, Amy Lynn Melok, Lee Lee, Siti Ayuni Mohamed Yussof, Tinchun Chu
Department of Biology Faculty Scholarship and Creative Works
Streptococcus mutans (S. mutans) is the main etiological bacteria present in the oral cavity that leads to dental caries. All of the S. mutans in the oral cavity form biofilms that adhere to the surfaces of teeth. Dental caries are infections facilitated by the development of biofilm. An esterified derivative of epigallocatechin-3-gallate (EGCG), epigallocatechin-3-gallate-stearate (EGCG-S), was used in this study to assess its ability to inhibit the growth and biofilm formation of S. mutans. The effect of EGCG-S on bacterial growth was evaluated with colony forming units (CFU) and log reduction; biofilm formation was qualitatively determined by Congo red assay, …
Cannabidiol Alters The Immune Response To Porphyromonas Gingivalis In Mice., 2018 University of Louisville
Cannabidiol Alters The Immune Response To Porphyromonas Gingivalis In Mice., Shilpa Singh
Electronic Theses and Dissertations
Background: Porphyromonas gingivalis is an important etiologic agent of chronic periodontitis, an infectious disease defined by destruction of supporting tissues of the teeth. Marijuana is a risk factor for chronic periodontitis, although underlying mechanisms are poorly understood. As phytocannabinoids have been ascribed antiinflammatory properties, we hypothesized that cannabidiol exposure would lead to altered immune response to P. gingivalis infection facilitating bacterial persistence and, subsequently, increased alveolar bone loss in cannabidiol (CBD)-exposed mice. Methods: P. gingivalis-infected cannabinoid receptor 2 (CB2) knockout and wild type C57/Bl6 mice were exposed, or not exposed, to cannabidiol. Oral swabs from mice were used to monitor …
Development Of Blended Polymeric Fibers To Alter The Release Of Peptide Targeting Porphyromonas Gingivalis., 2018 University of Louisville
Development Of Blended Polymeric Fibers To Alter The Release Of Peptide Targeting Porphyromonas Gingivalis., Sonali Suresh Sapare
Electronic Theses and Dissertations
Periodontitis is a chronic inflammatory disease that infects the tissues of the periodontium. It is estimated that 47.2% or 67.2 million American adults suffer from mild, moderate and severe periodontitis. Globally, 30-50% of the adult population is afflicted with periodontal disease, making it one of the most prevalent infectious diseases in the world. Therapeutics targeting P. gingivalis may be effective to alter periodontitis progression. However, the current treatment modalities that target critical pathogens to maintain host-biofilm homeostasis are limited, urging the development of specifically targeted therapeutics to limit P. gingivalis recolonization of the oral cavity after periodontal treatment and healing. …
Kallikrein-Related Peptidases Are Dysregulated In Pleomorphic Adenoma, 2018 The University of Western Ontario
Kallikrein-Related Peptidases Are Dysregulated In Pleomorphic Adenoma, Matthew D. Morrison
Electronic Thesis and Dissertation Repository
Objectives: Pleomorphic adenoma (PA) is the most common benign salivary gland tumor. Kallikrein-related peptidases (KLKs) have been identified as biomarkers in many human tumors and may influence tumor behavior. We investigated KLK1-15 messenger ribonucleic acid (mRNA) and proteins in PA specimens to determine a KLK expression profile for this tumor.
Methods: Fresh frozen PA tissue specimens (n = 26) and matched controls were subjected to RT-qPCR to detect KLK1-15 mRNA. Expression of KLK1, KLK12, KLK13 and KLK8 proteins were then evaluated via immunostaining techniques. Statistical analyses were performed with the level of significance set at P < .05.
Results: We observed downregulation …
Influence Of The Oral Microbiome On General Health, 2018 Touro College of Pharmacy, New York Medical College
Influence Of The Oral Microbiome On General Health, Zvi G. Loewy, Shoshana Galbut, Ephraim Loewy, David A. Felton
Touro College of Pharmacy (New York) Publications and Research
The prevalence of edentulism is common worldwide. While improvements in access to healthcare and dental care are reducing the prevalence rate of edentulism, the rapidly growing number of elderly as a percent of the global population will sustain a need for denture therapy for the foreseeable future. While denture use has positive impacts on the quality of life, their use is associated with some problems and risks. Denture stomatitis, a chronic infection-related inflammatory disorder of the oral mucosa, is extremely common and has been reported to occur in up to two-thirds of denture wearers. Importantly, epidemiology studies have shown edentulism …
The Effects Of Drug Abuse On Oral Health, 2018 Touro College
The Effects Of Drug Abuse On Oral Health, Betzalel Krasnow
The Science Journal of the Lander College of Arts and Sciences
Drug abuse, currently a national epidemic affecting millions of Americans, causes numerous health issues including increased dental disease. There are several factors which can contribute to an increase in caries and missing teeth. One of the most discussed mechanisms is xerostomia. Drug abusers tend to have large sugar intakes which exacerbates the problems caused by xerostomia. Drug abusers are also at risk for oral infections associated with dental decay due to their altered saliva composition. Unfortunately, once the symptoms are present there is little that can be done to correct them and the goal of the treating dentist should be …
Aloe Vera: A Multipurpose Healer And Bacterial Eradicator, 2018 Virginia Commonwealth University
Aloe Vera: A Multipurpose Healer And Bacterial Eradicator, Mary X. Tran, Leira N. Jimenez, Linda K. Blackburn
Dental Hygiene Student Scholarship
The Aloe vera plant is a succulent known for its rich content in vitamins and minerals, thus gaining popularity over the years in healthcare products. With advancements in alternative medicine, it has been recently found useful in dentistry due to properties such as anti-inflammatory, antioxidant and antimicrobial actions that contribute to wound healing. The purpose of this study was to examine and discover how Aloe vera can be used as an alternative therapy in the dental field. The PubMed, Google Scholar and Dentistry & Oral Sciences (DOSS) databases were utilized to find current scientific evidence on the effects of Aloe …
Comparative Oral+Ent Biology, 2018 University of the Pacific
Comparative Oral+Ent Biology, Marcos Gridi-Papp
Pacific Open Texts
Comparative Oral+ENT Biology is designed for a semester-long course taken by undergraduate students who are preparing for careers in dentistry, medicine, veterinary, audiology, speech pathology or evolutionary biology. It explores the mouth, ears, nose and throat of humans and animals discussing their evolution, development, function, and some common clinical issues. The text provides a broad background through an integrative and organismal perspective. It crosses the boundaries of disciplines, anatomical regions and professions to present structures and mechanisms within an evolutionary context. This textbook is richly illustrated with images made available at Wikimedia Commons. It contains materials from and links to …