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Marker-Trait Association Mapping Of Sorghum Mosaic Virus Resistance In Sugarcane, Chenie Zamora 2022 Louisiana State University and Agricultural and Mechanical College

Marker-Trait Association Mapping Of Sorghum Mosaic Virus Resistance In Sugarcane, Chenie Zamora

LSU Master's Theses

Mosaic is a potentially devastating disease of sugarcane that had severe economic impact on Louisiana’s sugarcane industry in the 1920’s and has caused periodic problems ever since. The disease is vectored by migrating aphids that transmit Sugarcane mosaic virus (SCMV) and Sorghum mosaic virus (SrMV) in a non-persistent manner. Mosaic in Louisiana is currently caused by strains of SrMV. Mosaic has been controlled primarily by developing resistant cultivars. Recently, mosaic was detected in some near-commercial clones in the cultivar selection program. This prompted extensive screening of breeding program parent populations that detected infiltration of susceptibility and revealed the need for …


Identification And Characterization Of Circular Rnas In Brassica Rapa In Response To Plasmodiophora Brassicae, Huishan Liu, Chinedu Charles Nwafor, Yinglan Piao, Xiaonan Li, Zongxiang Zhan, Zhongyun Piao 2022 Shenyang Agricultural University

Identification And Characterization Of Circular Rnas In Brassica Rapa In Response To Plasmodiophora Brassicae, Huishan Liu, Chinedu Charles Nwafor, Yinglan Piao, Xiaonan Li, Zongxiang Zhan, Zhongyun Piao

Center for Plant Science Innovation: Faculty and Staff Publications

Plasmodiophora brassicae is a soil-borne pathogen that attacks the roots of cruciferous plants and causes clubroot disease. CircRNAs are noncoding RNAs, widely existing in plant and animal species. Although knowledge of circRNAs has been updated continuously and rapidly, information about circRNAs in the regulation of clubroot disease resistance is extremely limited in Brassica rapa. Here, Chinese cabbage (BJN 222) containing clubroot resistance genes (CRa) against P. brassicae Pb4 was susceptible to PbE. To investigate the mechanism of cicRNAs responsible for clubroot disease resistance in B. rapa, circRNA-seq was performed with roots of ‘BJN 222’ at 0, 8, and 23 days …


Genetic Characterization Of Resistance To Bacterial Panicle Blight And Sheath Blight In Rice Using Qtl Linkage Analysis And Qtl-Seq, John Christian E. Ontoy 2022 Louisiana State University and Agricultural and Mechanical College

Genetic Characterization Of Resistance To Bacterial Panicle Blight And Sheath Blight In Rice Using Qtl Linkage Analysis And Qtl-Seq, John Christian E. Ontoy

LSU Master's Theses

Bacterial panicle blight (BPB) and sheath blight (SB) are major diseases of rice causing significant yield reduction in conducive conditions. Few cultivars have shown reliable resistance to these diseases. In this study, a recombinant inbred line (RIL) population derived from the U.S. cultivars Jupiter (moderately resistant) and Trenasse (susceptible) was investigated to identify genomic regions associated with BPB and SB resistance. Phenotypes in BPB and SB, as well as days to heading (DH), of the RIL population were evaluated in the field. DH was found to be correlated to BPB and SB diseases. BPB was also positively correlated to SB. …


Molecular Evidence Of Cryptic Hybridization In The Japanese Nezasa Bamboos (Pleioblastus Section Nezasa), Ryan Long 2022 Jacksonville State University

Molecular Evidence Of Cryptic Hybridization In The Japanese Nezasa Bamboos (Pleioblastus Section Nezasa), Ryan Long

Theses

The genus Pleioblastus is a complex group of Southeast Asian temperate bamboos with 7-21 species, depending on taxonomic authority. This study tests the hypothesis that taxonomic complexity in this group is due in part to hybridization and subsequent backcrossing in natural populations, resulting in a reticulate evolutionary history and a contemporary species assemblage that includes cryptic hybrids. This hypothesis is supported by recent research on the temperate bamboos that revealed intergeneric hybrids involving Pleioblastus and other genera of temperate bamboos (Triplett and Clark, 2021). The objective of the current study was to use data from Amplified Fragment Length Polymorphism (AFLP) …


Spectral Signature Description Of Some Wheat Genotypes And Study Their Relationship With Yield Prediction Spectraly In The Semi Arid Zones, توصيف البصمة الطيفية لبعض طرز القمح ودراسة علاقتها بتنبؤ الإنتاجية طيفياً في المناطق شبه الجافة, Nasser Ibrahem, Husam Farag 2022 Cereal Program, The Arab Center for the Studies of Arid Zones and Dry Lands / ACSAD.

Spectral Signature Description Of Some Wheat Genotypes And Study Their Relationship With Yield Prediction Spectraly In The Semi Arid Zones, توصيف البصمة الطيفية لبعض طرز القمح ودراسة علاقتها بتنبؤ الإنتاجية طيفياً في المناطق شبه الجافة, Nasser Ibrahem, Husam Farag

Arab Journal of Arid Environments المجلة العربية للبيئات الجافة

In this research, the spectral signature was determined within the wavelength range 350-2500 nm at every 1 nm during all growth stages of some wheat genotypes cultivated in the Arab Center for the Studies of Arid Zones and Dry lands (Sabboura, Damascus countryside, Syria) under the semi arid zones conditions in season 2018. This is to demonstrate the possibility of using them in preeding programs for productivity, as well as finding the relationship between the values of spectral reflectance during the different stages of growth with grain productivity according to mathematical equations based on the values of spectral indices recorded …


Effect Of Some Thermal Treatments In The Growth And Development Of Seedlings Carob (Ceratonia Siliqua L.), تأثير بعض المعاملات الحرارية في نمو وتطور بادرات الخرنوب Ceratonia Siliqua L., Samer Nasser, Talal Ameen, Hafez Mahfoud 2022 Department of Forestry and Ecology, Faculty of Agriculture, Tishreen University, Lattakia , Syria

Effect Of Some Thermal Treatments In The Growth And Development Of Seedlings Carob (Ceratonia Siliqua L.), تأثير بعض المعاملات الحرارية في نمو وتطور بادرات الخرنوب Ceratonia Siliqua L., Samer Nasser, Talal Ameen, Hafez Mahfoud

Arab Journal of Arid Environments المجلة العربية للبيئات الجافة

This research was conducted at the Scientific Agricultural Research Center (Lattakia/Syria), during the first half of 2015 In order to increase knowledge about the impact of several thermal treatments on some qualitative and quantitative indicators for the growth of shoot and root system of carob (Ceratonia siliqua L.) seedlings. three places were selected with different temperatures degree. The first (Lab, 25±0.5°C ) the second ( Room growth, 20.66±0.5°C ) the third (Open Field, 17.3±0.5°C ). And a private agricultural vessels (Minirhizotron) were used to follow the growth of seedlings roots. The results showed a superiority of Laboratory treatment at …


Multi-Year Data Analysis And Genomic Selection To Improve The Efficiency Of A Rice Breeding Program, Tommaso Cerioli 2022 Louisiana State University and Agricultural and Mechanical College

Multi-Year Data Analysis And Genomic Selection To Improve The Efficiency Of A Rice Breeding Program, Tommaso Cerioli

LSU Doctoral Dissertations

Plant breeding dramatically improved crops performances during human history and will play a pivotal role in shaping the future of agriculture. However, this activity requires large amount of time and resources. The availability of multi-year datasets and abundant DNA information enables new analyses and breeding approaches that can increase the productivity and efficiency of a breeding program. In recent years, the LSU Rice Breeding Program has implemented marker assisted selection (MAS) and genomic selection (GS) to predict performance of rice genotypes before field testing. The goal of this project was to conduct analyses and test molecular approaches to increase the …


Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism, Cintia Lucía Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah McHale, Thomas E. Clemente, Ana Paula Alonso, Chi Zhang 2022 University of North Texas

Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism, Cintia Lucía Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah Mchale, Thomas E. Clemente, Ana Paula Alonso, Chi Zhang

Center for Plant Science Innovation: Faculty and Staff Publications

Soybean oil is one of the most consumed vegetable oils worldwide. Genetic improvement of its concentration in seeds has been historically pursued due to its direct association with its market value. Engineering attempts aiming to increase soybean seed oil presented different degrees of success that varied with the genetic design and the specific variety considered. Understanding the embryo’s responses to the genetic modifications introduced, is a critical step to successful approaches. In this work, the metabolic and transcriptional responses to AtWRI1 and AtDGAT1 expression in soybean seeds were evaluated. AtWRI1 is a master regulator of fatty acid (FA) biosynthesis, and …


Arkansas Cotton Variety Test 2021, F. Bourland, A. Beach, B. Milano, C. Kennedy, L. Martin, B. Robertson 2022 University of Arkansas, Fayetteville

Arkansas Cotton Variety Test 2021, F. Bourland, A. Beach, B. Milano, C. Kennedy, L. Martin, B. Robertson

Arkansas Agricultural Experiment Station Research Series

The primary goal of the Arkansas Cotton Variety Test is to provide unbiased data regarding the agronomic performance of cotton varieties and advanced breeding lines in the major cotton-growing areas of Arkansas. This information helps seed companies establish marketing strategies and assists producers in choosing varieties to plant. These annual evaluations will then facilitate the inclusion of new, improved genetic material in Arkansas cotton production. Adaptation of varieties is determined by evaluating the lines at five University of Arkansas System Division of Agriculture research sites (Manila, Keiser, Judd Hill, Marianna, and Rohwer). The 2021 tests at Rohwer were abandoned due …


Fddm1 And Fddm2, Two Sgs3-Like Proteins, Function As A Complex To Affect Dna Methylation In Arabidopsis, Shengjun Li, Weilong Yang, Yunfeng Liu, Guangyong Li, Xiang Liu, Yaling Liu, James R. Alfano, Chi Zhang, Bin Yu 2022 Chinese Academy of Sciences; University of Nebraska-Lincoln

Fddm1 And Fddm2, Two Sgs3-Like Proteins, Function As A Complex To Affect Dna Methylation In Arabidopsis, Shengjun Li, Weilong Yang, Yunfeng Liu, Guangyong Li, Xiang Liu, Yaling Liu, James R. Alfano, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty and Staff Publications

DNA methylation is an important epigenetic modification required for the specific regulation of gene expression and the maintenance of genome stability in plants and animals. However, the mechanism of DNA demethylation remains largely unknown. Here, we show that two SGS3-like proteins, FACTOR OF DNA DEMETHYLATION 1 (FDDM1) and FDDM2, negatively affect the DNA methylation levels at ROS1-dependend DNA loci in Arabidopsis. FDDM1 binds dsRNAs with 50 overhangs through its XS (rice gene X and SGS3) domain and forms a heterodimer with FDDM2 through its XH (rice gene X Homology) domain. A lack of FDDM1 or FDDM2 increased DNA methylation levels …


Qteller: A Tool For Comparative Multi-Genomic Gene Expression Analysis, Margaret R. Woodhouse, Shatabdi Sen, David Schott, John L. Portwood II, Michael Freeling, Justin W. Walley, Carson M. Andorf, James Schnable 2022 USDA ARS, Ames, IA

Qteller: A Tool For Comparative Multi-Genomic Gene Expression Analysis, Margaret R. Woodhouse, Shatabdi Sen, David Schott, John L. Portwood Ii, Michael Freeling, Justin W. Walley, Carson M. Andorf, James Schnable

Center for Plant Science Innovation: Faculty and Staff Publications

Motivation: Over the last decade, RNA-Seq whole-genome sequencing has become a widely used method for measuring and understanding transcriptome-level changes in gene expression. Since RNA-Seq is relatively inexpensive, it can be used on multiple genomes to evaluate gene expression across many different conditions, tissues and cell types. Although many tools exist to map and compare RNA-Seq at the genomics level, few web-based tools are dedicated to making data generated for individual genomic analysis accessible and reusable at a gene-level scale for comparative analysis between genes, across different genomes and meta-analyses. Results: To address this challenge, we revamped the comparative gene …


Proceedings Of The 2022 Passion Fruit Conference: Growing The U.S. Passion Fruit Industry A Strategic Conference For: Growers, Marketers, Researchers, And Stakeholders, Eric T. Stafne 2022 Mississippi State University

Proceedings Of The 2022 Passion Fruit Conference: Growing The U.S. Passion Fruit Industry A Strategic Conference For: Growers, Marketers, Researchers, And Stakeholders, Eric T. Stafne

Coastal Research and Extension Center Publications

We are pleased to deliver these proceedings of the passion fruit conference that was held in Homestead, Florida on June 14 and 15, 2022. It was a culmination of a lot of hard work by many people, most of them listed in the previous page. However, it would not have been a success without the attendees who came from various parts of the country to share their experiences growing passion fruit. What follows in these proceedings are the slides of the presentations with by notes taken by attendees along with notes taken of discussion regarding the five priority topic areas: …


Berry Anthocyanin, Acid, And Volatile Trait Analyses In A Grapevine-Interspecific F2 Population Using An Integrated Gbs And Rhampseq Genetic Map, Dilmini Alahakoon, Anne Fennell, Zachary Helget, Terry Bates, Avainash Karn, David Manns, Anna Katherine Mansfield, Bruce I. Reisch, Gavin Sacks, Qi Sun, Cheng Zou, Lance Cadle-Dvidson, Jason P. Londo 2022 South Dakota State University

Berry Anthocyanin, Acid, And Volatile Trait Analyses In A Grapevine-Interspecific F2 Population Using An Integrated Gbs And Rhampseq Genetic Map, Dilmini Alahakoon, Anne Fennell, Zachary Helget, Terry Bates, Avainash Karn, David Manns, Anna Katherine Mansfield, Bruce I. Reisch, Gavin Sacks, Qi Sun, Cheng Zou, Lance Cadle-Dvidson, Jason P. Londo

Agronomy, Horticulture and Plant Science Faculty Publications

Increased map density and transferability of markers are essential for the genetic analysis of fruit quality and stress tolerance in interspecific grapevine populations. We used 1449 GBS and 2000 rhAmpSeq markers to develop a dense map for an interspecific F2 population (VRS-F2) that was derived by selfing a single F1 from a Vitis riparia x ‘Seyval blanc’ cross. The resultant map contained 2519 markers spanning 1131.3 cM and was highly collinear with the Vitis vinifera ‘PN40024’ genome. Quantitative trait loci (QTL) for berry skin color and flower type were used to validate the map. Four rhAmpSeq …


The Challenges Of Growing Orchids From Seeds For Conservation: An Assessment Of Asymbiotic Techniques, Devani Jolman, Martín I. Batalla, Alexis Hungerford, Pryce Norwood, Noah Tait, Lisa E. Wallace 2022 Old Dominion University

The Challenges Of Growing Orchids From Seeds For Conservation: An Assessment Of Asymbiotic Techniques, Devani Jolman, Martín I. Batalla, Alexis Hungerford, Pryce Norwood, Noah Tait, Lisa E. Wallace

Biological Sciences Faculty Publications

Lewis Knudson first successfully germinated orchid seeds asymbiotically on artificial medium in 1922. While many orchid species have since been grown asymbiotically, the tremendous variation in how species respond to artificial medium and growth conditions ex situ has also become apparent in the past century. In this study, we reviewed published journal articles on asymbiotic orchid seed germination to provide a summary of techniques used and to evaluate if these differ between terrestrial and epiphytic species, to identify areas where additional research is needed, and to evaluate whether asymbiotic germination could be used more often in ex situ conservation. We …


Keeping What You Sow: Intellectual Property Rights For Plant Breeders And Seed Growers, Paulina B. Jenney 2022 University of Montana

Keeping What You Sow: Intellectual Property Rights For Plant Breeders And Seed Growers, Paulina B. Jenney

Graduate Student Theses, Dissertations, & Professional Papers

Over the last 150 years, the food system in the present-day United States has undergone a transformational restructuring, from a diversified, decentralized, network of farmers and seed growers, to one in which the majority of crop production is controlled by a few industrial corporations. The consolidation of power has been under-girded by the application of intellectual property rights (IPR)—especially utility patents—to plant varieties and genetic traits, which are leveraged to exclude small-scale seed growers from accessing quality germplasm. Patents and restrictive licensing agreements recapitulate colonial structures by appropriating common and traditionally community-held resources for profit, and by creating reliance on …


Genomic Analysis Of Pollen Grains For Forensic Applications, Luz Kelley 2022 University of Central Florida

Genomic Analysis Of Pollen Grains For Forensic Applications, Luz Kelley

Electronic Theses and Dissertations, 2020-

With over 300,000 plant species on the planet and more than 90% of them relying on pollen for reproduction, palynology, the study of pollen grains plays a vital role in many research fields. One of them is forensic palynology, which uses pollen as a proxy to link individuals or objects to a location or instance. It relies on the fact that (1) pollen is an ever-present feature of the environment; (2) different locations have different pollen signatures, allowing for inference related to spatial tracking; (3) plants bloom at different times, allowing for temporal inference; and (4) pollen is exceptionally durable …


Determinants Of Population Genetic Structure In Chamaecrista Fasciculata (Michx.) Greene (Fabaceae) In The Southeastern United States, Mahboubeh Hosseinalizadeh Nobarinezhad, Lisa E. Wallace 2022 Old Dominion University

Determinants Of Population Genetic Structure In Chamaecrista Fasciculata (Michx.) Greene (Fabaceae) In The Southeastern United States, Mahboubeh Hosseinalizadeh Nobarinezhad, Lisa E. Wallace

Biological Sciences Faculty Publications

Chamaecrista fasciculata is a widely distributed, phenotypically variable species in the eastern U.S. Whereas studies have demonstrated genetic structure and local adaptation in northern areas of its distribution, there has been no comparison of genetic variability among populations at the southern extent where phenotypic variation is more complex. We characterized genetic variation at 14 microsatellite loci for populations in Mississippi and Alabama and compared this to variation in a phenotypic trait, leaf pubescence. Geographic distance, climatic variables, and elevation were evaluated as factors to explain the observed patterns of genetic diversity. A significant amount of variation (19%) resided among populations, …


Development Of Genomic Resources In Vitis Riparia For Discoveries On Pre- And Post-Transcriptional Molecular Regulators Of Early Induction Into Endodormancy, Michael Robben 2022 South Dakota State University

Development Of Genomic Resources In Vitis Riparia For Discoveries On Pre- And Post-Transcriptional Molecular Regulators Of Early Induction Into Endodormancy, Michael Robben

Electronic Theses and Dissertations

Grapevine is one of the most important fruit crops in the world, responsible for billions in global sales annually. The largest threat to grapevine and other crop production is global climate change resulting human activities. This brings unpredictable and drastic changes in ambient air temperatures to many climates in which grapes are grown. Lower temperatures and inclement weather are already responsible for millions in lost revenue due to tissue damage of established plants. Thus, protecting grapevine crops from weather-related damage is the biggest concern to growers aside from pathogen- and diseaserelated crop damage. The primary mechanism for winter survival in …


Genome-Wide Association Mapping And Genomic Prediction For Enhancing Fhb Resistance In Hard Winter Wheat, Jinfeng Zhang 2022 South Dakota State University

Genome-Wide Association Mapping And Genomic Prediction For Enhancing Fhb Resistance In Hard Winter Wheat, Jinfeng Zhang

Electronic Theses and Dissertations

Wheat is one of the most important staple crops providing 20% of energy for 35% of the world population. Fusarium head blight (FHB), primarily caused by the fugal Fusarium graminearum Schwabe, is a damaging disease in wheat that affects global wheat production every year and causes food safety issues. The disease not only reduces the grain yield and quality but also produces mycotoxin in the diseased kernels making them unsuitable for human consumption or as livestock feeds. Breeding FHB resistant cultivar is the most effective and economical approach to managing the disease. This study combines genome-wide association study (GWAS) and …


Characterizing Drought Adaptations, Phenotypic Plasticity, And Fixed Gene Expression Patterns Within Quercus, James K. Rauschendorfer 2022 Michigan Technological University

Characterizing Drought Adaptations, Phenotypic Plasticity, And Fixed Gene Expression Patterns Within Quercus, James K. Rauschendorfer

Dissertations, Master's Theses and Master's Reports

This dissertation was written on topics related to the genus Quercus with a primary focus on Quercus ellipsoidalis (northern pin oak) and Quercus rubra (northern red oak). Within this dissertation are chapters related to the setup of experimental common gardens within the Ford and Kellogg experimental forest, a literature review describing drought adaptations of Quercus sect. Lobatae (red oak group), identification of transcription factors within the Q. robur (English oak) and Q. rubra genomes, a study comparing leaf trait phenotypic plasticity of Q. ellipsoidalis and Q. rubra, and an RNA-seq experiment studying ecological speciation between Q. ellipsoidalis and Q. …


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