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Machine Learning And Rna Bioinformatics, Jason Rafe Miller 2024 West Virginia University

Machine Learning And Rna Bioinformatics, Jason Rafe Miller

Graduate Theses, Dissertations, and Problem Reports (ETD)

The applied science of bioinformatics encompasses computational analysis of molecular biology data. Advances in genomics and DNA sequencing technology have enabled computational analysis of ribonucleic acids (RNAs), which play diverse and critical roles in most cells. To assist the study of human RNA, we trained machine learning models on RNA nucleotide sequences, devoid of domain knowledge. We built models that distinguish long non-coding lncRNA from protein-coding mRNA, and models that predict the cytoplasmic vs. nuclear preferences of lncRNAs. In a review of published lncRNA subcellular localization classifiers, we show that the commonly used validation protocol generates optimistic performance measures, and …


Evaluation Of Five Roma Tomato Cultivars In Southwest Michigan In 2023, Ben Phillips, Jenny Schoonmaker 2023 Michigan State University

Evaluation Of Five Roma Tomato Cultivars In Southwest Michigan In 2023, Ben Phillips, Jenny Schoonmaker

Midwest Vegetable Trial Reports

A Roma tomato cultivar trial was planted at the Southwest Michigan Research and Extension Center (42.081985, -86.354087, Benton Harbor, Michigan). HM Claus (HM), PanAmerican (PA), and Sakata (SK) seed companies donated five Roma tomato cultivars for plastic-mulch bedded and trellised hand harvest. Overall trial quality was excellent, despite a dry start that brought in insects, and hard rains that flared bacterial diseases.


Evaluation Of 13 Round Tomato Cultivars In Southwest Michigan In 2023, Ben Phillips, Jenny Schoonmaker 2023 Michigan State University

Evaluation Of 13 Round Tomato Cultivars In Southwest Michigan In 2023, Ben Phillips, Jenny Schoonmaker

Midwest Vegetable Trial Reports

A round tomato cultivar trial was planted at the Southwest Michigan Research and Extension Center (42.081985, -86.354087, Benton Harbor, Michigan). Bejo (BJ), Enza Zaden (EZ), Seminis (SM), Sakata (SK), and Seedway (SW) seed companies donated round tomato cultivars for plastic-mulch bedded and trellised hand harvest. Overall trial quality was excellent, despite a dry start that brought in insects, and hard rains that flared bacterial diseases.


Evaluation Of Five Cauliflower Cultivars For Summer Harvest In Southwest Michigan In 2023, Ben Phillips, LEah Freeman 2023 Michigan State University

Evaluation Of Five Cauliflower Cultivars For Summer Harvest In Southwest Michigan In 2023, Ben Phillips, Leah Freeman

Midwest Vegetable Trial Reports

A cauliflower cultivar trial was planted at the Southwest Michigan Research and Extension Center (42.081985, -86.354087, Benton Harbor, Michigan). Bejo (BJ), Clifton (CL), Sakata (SK), and Tozer (TZ) seed companies donated five cauliflower cultivars for plastic-mulch bedded hand harvest. The trial quality was good for collecting data on stressed plants. A dry start and cabbage maggot attack reduced stand with replacement plants lagging behind, and heat around head formation created a high proportion of non-marketable heads.


Evaluation Of 11 Broccoli Cultivars For Summer Harvest In Southwest Michigan In 2023, Ben Phillips, Leah Freeman 2023 Michigan State University

Evaluation Of 11 Broccoli Cultivars For Summer Harvest In Southwest Michigan In 2023, Ben Phillips, Leah Freeman

Midwest Vegetable Trial Reports

A broccoli cultivar trial was planted at the Southwest Michigan Research and Extension Center (42.081985, -86.354087, Benton Harbor, Michigan). Bejo (BJ), Clifton (CL), Sakata (SK), and Tozer (TZ) seed companies donated 11 broccoli cultivars for plastic-mulch bedded hand harvest. The trial quality was good for collecting data on stressed plants. A dry start and cabbage maggot attack reduced stand with replacement plants lagging behind, and heat around head formation created a high proportion of non-marketable heads.


Evaluation Of Six Slicing Cucumber Cultivars In Southwest Michigan In 2023, Ben Phillips, Jenny Schoonmaker 2023 Michigan State University

Evaluation Of Six Slicing Cucumber Cultivars In Southwest Michigan In 2023, Ben Phillips, Jenny Schoonmaker

Midwest Vegetable Trial Reports

A slicing cucumber cultivar trial was planted at the Southwest Michigan Research and Extension Center (42.081985, -86.354087, Benton Harbor, Michigan). Clifton (CL), Seedway (SW), Seminis (SM), and Syngenta (SY) seed companies donated five slicing cucumber cultivars for plastic-mulch bedded hand harvest. The trial quality was OK, with a dry start that created an uneven stand, and some deer browse.


Soybean Genetics, Genomics, And Breeding For Improving Nutritional Value And Reducing Antinutritional Traits In Food And Feed, William M. Singer, Yi-Chen Lee, Zachary Shea, Caio Canella Vieira, Dongho Lee, Xiaoying Li, Mia Cunicelli, Shaila S. Kadam, Mohammad Amir Waseem Khan, Grover Shannon, M. A. Rouf Mian, Henry T. Nguyen, Bo Zhang 2023 Virginia Polytechnic Institute and State University

Soybean Genetics, Genomics, And Breeding For Improving Nutritional Value And Reducing Antinutritional Traits In Food And Feed, William M. Singer, Yi-Chen Lee, Zachary Shea, Caio Canella Vieira, Dongho Lee, Xiaoying Li, Mia Cunicelli, Shaila S. Kadam, Mohammad Amir Waseem Khan, Grover Shannon, M. A. Rouf Mian, Henry T. Nguyen, Bo Zhang

Agriculture Faculty Publications

Soybean [Glycine max(L.) Merr.] is a globally important crop due to its valuable seed composition, versatile feed, food, and industrial end-uses, and consistent genetic gain. Successful genetic gain in soybean has led to widespread adaptation and increased value for producers, processors, and consumers. Specific focus on the nutritional quality of soybean seed composition for food and feed has further elucidated genetic knowledge and bolstered breeding progress. Seed components are historical and current targets for soybean breeders seeking to improve nutritional quality of soybean. This article reviews genetic and genomic foundations for improvement of nutritionally important traits, such as protein and …


Origin, Rise And Development Of American Upland Cotton And Their Status At Present. Second Edition, Ed. By F.M. Bourland, J. O. Ware 2023 University of Arkansas, Fayetteville

Origin, Rise And Development Of American Upland Cotton And Their Status At Present. Second Edition, Ed. By F.M. Bourland, J. O. Ware

AAES Research Reports and Research Bulletins

American Upland cotton (Gossypium hirsutum) is a Dixie product. Although the stocks of the species were brought from elsewhere, new types (through series of adaptational changes) formed this distinctive group—the final characteristics of which are a product of the Cotton Belt of the United States. These biological processes were considerably aided by man and the steps of development possibly were about as follows: (a) natural selection took place in the earlier introductions, (b) seed were saved from the more choice stocks, (c) series of subsequent introductions were obtained from the better sources, (d) these were acclimatized and the superior ones …


Mycorrhizal Feedbacks Influence Global Forest Structure And Diversity, Camille S. Delavaux, Joseph A. LaManna, Jonathan A. Myers, Richard P. Phillips, Salomón Aguilar, David Allen, Alfonso Alonso, Kristina J. Anderson-Teixeira, Matthew E. Baker, Jennifer L. Baltzer, Pulchérie Bissiengou, Mariana Bonfim, Norman A. Bourg, Warren Y. Brockelman, David F.R.P. Burslem, Li Wan Chang, Yang Chen, Jyh Min Chiang, Chengjin Chu, Keith Clay, Susan Cordell, Mary Cortese, Jan den Ouden, Christopher Dick, Sisira Ediriweera, Erle C. Ellis, Anna Feistner, Amy L. Freestone, Thomas Giambelluca, Christian P. Giardina, Gregory S. Gilbert, Fangliang He, Jan Holík, Robert W. Howe, Walter Huaraca Huasca, Stephen P. Hubbell, Faith Inman, Patrick A. Jansen, Daniel J. Johnson, Kamil Kral, Sabrina E. Russo, et al. 2023 ETH Zürich

Mycorrhizal Feedbacks Influence Global Forest Structure And Diversity, Camille S. Delavaux, Joseph A. Lamanna, Jonathan A. Myers, Richard P. Phillips, Salomón Aguilar, David Allen, Alfonso Alonso, Kristina J. Anderson-Teixeira, Matthew E. Baker, Jennifer L. Baltzer, Pulchérie Bissiengou, Mariana Bonfim, Norman A. Bourg, Warren Y. Brockelman, David F.R.P. Burslem, Li Wan Chang, Yang Chen, Jyh Min Chiang, Chengjin Chu, Keith Clay, Susan Cordell, Mary Cortese, Jan Den Ouden, Christopher Dick, Sisira Ediriweera, Erle C. Ellis, Anna Feistner, Amy L. Freestone, Thomas Giambelluca, Christian P. Giardina, Gregory S. Gilbert, Fangliang He, Jan Holík, Robert W. Howe, Walter Huaraca Huasca, Stephen P. Hubbell, Faith Inman, Patrick A. Jansen, Daniel J. Johnson, Kamil Kral, Sabrina E. Russo, Et Al.

Center for Plant Science Innovation: Faculty Publications

One mechanism proposed to explain high species diversity in tropical systems is strong negative conspecific density dependence (CDD), which reduces recruitment of juveniles in proximity to conspecific adult plants. Although evidence shows that plant-specific soil pathogens can drive negative CDD, trees also form key mutualisms with mycorrhizal fungi, which may counteract these effects. Across 43 large-scale forest plots worldwide, we tested whether ectomycorrhizal tree species exhibit weaker negative CDD than arbuscular mycorrhizal tree species. We further tested for conmycorrhizal density dependence (CMDD) to test for benefit from shared mutualists. We found that the strength of CDD varies systematically with mycorrhizal …


Enabling Breeding For Fruitlet Freeze Tolerance In Peach, Ufuk Caglar 2023 Clemson University

Enabling Breeding For Fruitlet Freeze Tolerance In Peach, Ufuk Caglar

All Theses

Climate change is affecting the production of temperate fruit crops, with cold temperatures emerging as a critical abiotic stressor that limits plant growth and performance. Freeze damage, particularly in spring, has resulted in significant economic losses in peach production in the southeastern United States. Research efforts in peach and other Prunus species have primarily focused on studying dormancy-related traits associated with bloom time, such as chill and heat requirement, with fruitlet freeze tolerance not equally represented. Breeding for climate resilience in peach requires a combination of these traits to allow for late bloom via targeted chill requirement and high heat …


Evaluation Of Vegetative Indices To Determine Canopy Ground Cover For Winter Survival And Hybrid Necrosis In Winter Wheat, Micheal Young 2023 University of Nebraska-Lincoln

Evaluation Of Vegetative Indices To Determine Canopy Ground Cover For Winter Survival And Hybrid Necrosis In Winter Wheat, Micheal Young

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The benefit of unmanned aircraft systems and image processing methods in agronomic research across numerous crops has been well documented as has the importance of wheat, Triticum aestivum L., on the global food supply. Hence there is great interest in digital solutions applied to aspects of wheat breeding. A major trait of importance to winter wheat breeders in higher latitudes is winter survival, which can result in poor yield and performance if lines do not survive extreme cold. Scoring winter survival is most commonly based on visual score of 0% to 100% with the higher percentage conveying higher winter survival …


Evaluation Of Oxyfluorfen For Use In Roxy Rice, Casey Hunter Arnold 2023 University of Arkansas, Fayetteville

Evaluation Of Oxyfluorfen For Use In Roxy Rice, Casey Hunter Arnold

Graduate Theses and Dissertations

Mid-southern U.S. rice (Oryza sativa L.) producers are challenged every year to control problematic barnyardgrass [Echinochloa crus-galli (L.) Beauv.] and weedy rice (Oryza sativa L.) because they have evolved resistance to most labeled herbicides. Oxyfluorfen is a Herbicide Resistance Action Committee (HRAC)/Weed Science Society of America (WSSA) Group 14 herbicide that inhibits protoporphyrinogen oxidase within the plant. Currently, there are no HRAC/WSSA Group 14 herbicides labeled for barnyardgrass or weedy rice control in midsouthern rice production. Therefore, oxyfluorfen is being evaluated for barnyardgrass and weedy rice control. Plant breeders at the University of California – Davis were able to breed …


Arkansas Soybean Research Studies 2022, Jeremy Ross 2023 University of Arkansas, Fayetteville

Arkansas Soybean Research Studies 2022, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading soybean-producing state in the mid-southern United States. Arkansas ranked 11th in soybean production in 2022 when compared to the other soybean-producing states in the U.S. The state represented 3.04% of the total U.S. soybean production and 3.64% of the total acres planted in soybean in 2022. The 2022 state soybean average yield was 52.0 bushels per acre, tying the previous state yield record of 52 bushels per acre set in 2021. The top five soybean-producing counties in 2022 were Mississippi, Crittenden, Phillips, Poinsett, and Arkansas (Table 1). These five counties accounted for over 35.7% of the …


A Genome Wide Association Study Of Heat Tolerance In Snap Bean (Phaseolus Vulgaris), Morgan Stone 2023 Clemson University

A Genome Wide Association Study Of Heat Tolerance In Snap Bean (Phaseolus Vulgaris), Morgan Stone

All Theses

Bean production in the United States has decreased while the temperatures have been steadily increasing, reaching new highs each year. Heat stress is detrimental to common bean (also known as snap bean) production. Symptoms of heat stress include decrease in pollen viability, shriveling of pods, and pod abortion making them unmarketable. Pod production of 323 snap bean accessions from a large diversity panel was assessed in a randomized complete block design with field trials at two different times in the spring season. The results show a significant decrease in the number of pods produced per plant and weight of pods …


Corn And Grain Sorghum Performance Tests 2023, J. F. Carlin, R. B. Mulloy, R. D. Bond, J. C. McCoy 2023 University of Arkansas, Fayetteville

Corn And Grain Sorghum Performance Tests 2023, J. F. Carlin, R. B. Mulloy, R. D. Bond, J. C. Mccoy

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

The 2023 corn performance tests contained 46 hybrids and were conducted at the Northeast Rice Research and Extension Center (NERREC) at Harrisburg, the Northeast Rice Research and Extension Center (NEREC) at Keiser, the Lon Mann Cotton Research Station (LMCRS) near Marianna, the Rohwer Research Station (RRS) near Rohwer, and the Rice Research and Extension Center (RREC) …


Integrated Techniques To Identify And Characterize Resistance To Fusarium Wilt Caused By Fusarium Oxysporum, F. Sp. Vasinfectum Race 4 In Two Domesticated Gossypium Species, Stephen Parris 2023 Clemson University

Integrated Techniques To Identify And Characterize Resistance To Fusarium Wilt Caused By Fusarium Oxysporum, F. Sp. Vasinfectum Race 4 In Two Domesticated Gossypium Species, Stephen Parris

All Dissertations

Fusarium wilt of cotton (Gossypium hirsutum and G. barbadense), caused by Fusarium oxysporum f. sp. vasinfectum race 4 (FOV4), is the most threatening disease faced by today’s cotton breeders. During the past 20 years, over $7 million have been dedicated to efforts in breeding upland cotton for resistance to FOV4 while yielding little to no results. To counteract this, we conducted a study in Clint, TX, that utilized control plots and geostatistical methods to adjust phenotypic data for the heterogeneous distribution of FOV4 in the field, improving the accuracy of genetic resistance assessments. Concurrently, early-generation genetic mapping in …


Advances And Opportunities In Unraveling Cold-Tolerance Mechanisms In The World's Primary Staple Food Crops, Sofora Jan, Sachin Rustgi, Rutwik Barmukh, Asif B. Shikari, Brenton Leske, Amanuel Bekuma, Darshan L. Sharma, Wujun Ma, Upendra Kumar, Uttam Kumar, Abhishek Bohra, Rajeev K. Varshney, Reyazul Rouf Mir 2023 Faculty of Agriculture (FoA), Sopore Kashmir, India

Advances And Opportunities In Unraveling Cold-Tolerance Mechanisms In The World's Primary Staple Food Crops, Sofora Jan, Sachin Rustgi, Rutwik Barmukh, Asif B. Shikari, Brenton Leske, Amanuel Bekuma, Darshan L. Sharma, Wujun Ma, Upendra Kumar, Uttam Kumar, Abhishek Bohra, Rajeev K. Varshney, Reyazul Rouf Mir

Grain and Other Field Crops Research Articles

Temperatures below or above optimal growth conditions are among the major stressors affecting productivity, end-use quality, and distribution of key staple crops including rice (Oryza sativa), wheat (Triticum aestivum), and maize (Zea mays L.). Among temperature stresses, cold stress induces cellular changes that cause oxidative stress and slowdown metabolism, limit growth, and ultimately reduce crop productivity. Perception of cold stress by plant cells leads to the activation of cold-responsive transcription factors and downstream genes, which ultimately impart cold tolerance. The response triggered in crops to cold stress includes gene expression/suppression, the accumulation of sugars upon chilling, and signaling molecules, among …


Janus , A Spliceosome-Associated Protein, Promotes Mirna Biogenesis In Arabidopsis, Mu Li, Huihui Yu, Bangjun Zhou, Lu Gan, Shengjun Li, Chi Zhang, Bin Yu 2023 University of Nebraska-Lincoln

Janus , A Spliceosome-Associated Protein, Promotes Mirna Biogenesis In Arabidopsis, Mu Li, Huihui Yu, Bangjun Zhou, Lu Gan, Shengjun Li, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

MicroRNAs ( miRNAs ) are important regulators of genes expression. Their levels are precisely controlled through modulating the activity of the microprocesser complex ( MC ) . Here, we report that JANUS, a homology of the conserved U2 snRNP assembly factor in yeast and human, is required for miRNA accumulation. JANUS associates with MC components Dicer-like 1 ( DCL1 ) and SERRATE ( SE ) and directly binds the stem- loop of pri-miRNAs. In a hypomorphic janus mutant, the activity of DCL1, the numbers of MC, and the interaction of primary miRNA transcript ( pri-miRNAs ) with MC are reduced. …


Quizalofop Resistance In Weedy Rice (Oryza Sativa L.) Is Mainly Conferred By An Ile1781leu Mutation, Fidel Gonzalez-Torralva, Jason K. Norsworthy 2023 University of Arkansas, Fayetteville

Quizalofop Resistance In Weedy Rice (Oryza Sativa L.) Is Mainly Conferred By An Ile1781leu Mutation, Fidel Gonzalez-Torralva, Jason K. Norsworthy

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Weedy rice (Oryza sativa L.) is an economically important weed species in rice (Oryza sativa L.) cropping systems. Two weedy rice samples (acc7 and acc8) suspected to be resistant to quizalofop-ethyl (quizalofop) were collected in Arkansas. In this research, susceptibility to quizalofop and resistance mechanisms have been explored. Dose-response assays displayed a resistance index of 42- and 58-fold for the acc7 and acc8, respectively. Experiments with metabolism inhibitors demonstrated that NBD-Cl (4-chloro-7-nitrobenzofurazan) increased quizalofop efficacy slightly in acc8, whereas malathion did not improve effectiveness in resistant samples. Sequencing of the ACCase gene displayed an Ile1781Leu substitution in the …


Arkansas Wheat Performance Tests 2022-2023, J. F. Carlin, R. B. Mulloy, R. D. Bond 2023 University of Arkansas, Fayetteville

Arkansas Wheat Performance Tests 2022-2023, J. F. Carlin, R. B. Mulloy, R. D. Bond

Arkansas Agricultural Experiment Station Research Series

Wheat variety performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture’s Arkansas Crop Variety Improvement Program.

The tests provide information to companies developing cultivars and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating variety recommendations for small-grain producers. The tests were conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Pine Tree Research Station near Colt, and the Rohwer Research Station near Rohwer. Specific location and cultural practice information accompany each …


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