Plant Breeding and Genetics Commons^™

Genetic Improvement Of Tocotrienol Content Enhances The Oxidative Stability Of Canola Oil, Min Deng, Hao Chen, Wei Zhang, Edgar B. Cahoon, Yongming Zhou, Chunyu Zhang

Center for Plant Science Innovation: Faculty Publications

Background: Tocotrienols and tocopherols, which are synthesized in plastids of plant cells with similar functionalities, comprise vitamin E to serve as a potent lipid-soluble antioxidant in plants. The synthesis of tocopherols involves the condensation of homogentisic acid (HGA) and phytyl diphosphate (PDP) under the catalysis of homogentisate phytyltransferase (HPT). Tocotrienol synthesis is initiated by the condensation of HGA and geranylgeranyl diphosphate (GGDP) mediated by homogentisate geranylgeranyl transferase (HGGT). As one of the most important oil crops, canola seed is regarded as an ideal plant to efficiently improve the production of vitamin E tocochromanols through genetic engineering approaches. However, only …

An Improved Digestion And Analysis Procedure For Silicon In Plant Tissue, Noah James Langenfeld, Bruce Bugbee

Plants, Soils, and Climate Faculty Publications

Silicon (Si) in plant tissues reduces abiotic and biotic stress, but it is incorporated as silica (SiO₂), which is difficult to solubilize for analysis. We modified an oven-induced tissue-digestion and analysis method to improve Si solubilization and validated its accuracy by quantifying the mass-balance recovery of Si from the hydroponic solution and plant tissues of cucumber (Cucumis sativus). Leaf, stem, and root tissues were dried, finely-ground, and digested in 12.5 molar sodium hydroxide at 95°C for 4 hours. Solutions were then acidified with 6 molar hydrochloric acid to achieve a pH below 2 for measurement of …

Driscoll’S Water-Use Efficiency (Wue) Strawberry Breeding Trial, Aidan Inoue

Plant Sciences

California’s water supply is seeing stress and growers are increasingly under pressure to lower their water use. While reducing irrigation to strawberry plants can be used to raise sugars (°bx), research on the impact of deficit irrigation on plant growth, fruit yield and quality are not yet quantified. The purpose of this experiment is to observe the effects of a ~50% deficit irrigation treatment on tabletop strawberry plants where drainage measurements and plant absorption can be recorded. Results indicate that plant vigor and total marketable yield are predominantly treatment dependent whereas fruit characteristics, including culls and berry size are reliant …

Summaries Of Arkansas Cotton Research 2022, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

The current economic environment continues to drive the need to produce record or near-record yields to be profitable. Price volatility in 2022 added another level of difficulty in the quest for being profitable. The cotton market saw significant movement after reaching a $1.5802 per pound high in May 2022, cotton prices corrected and more than halved in value at the October 2022 low, and then consolidated around the 80 cents per pound level in December 2022 (Cotton is Consolidating). Great uncertainties still exist for the upcoming season, most of which are outside of our control. These include, but are not …

Maize Leaf Angle Genetic Gain Is Slowing Down In The Last Decades, Elvis F. Elli, Jode Edwards, Jianming Yu, Slobodan Trifunovic, Douglas M. Eudy, Kevin R. Kosola, Patrick S. Schnable, Kendall R. Lamkey, Sotirios V. Archontoulis

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Quantifying historical changes in maize leaf angle and factors affecting it can enhance our understanding of canopy architecture and light capture, and hence crop productivity. Our objectives were to (1) quantify leaf angle genetic gain per canopy position in Bayer's legacy maize (Zea mays L.) hybrids; (2) dissect the contribution of breeding from plant density on historical changes in leaf angle; and (3) synthesize our findings with literature to determine leaf angle changes over a century of breeding. We measured leaf angle in 78 maize hybrids released between 1980 and 2020 across eight environments in the US Corn Belt. …

Exome-Based New Allele-Specific Pcr Markers And Transferability For Sodicity Tolerance In Bread Wheat (Triticum Aestivum L.), Roopali Bhoite, Rosemary Smith, Urmil Bansal, Mirza Dowla, Harbans Bariana, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Targeted exome-based genotype by sequencing (t-GBS), a sequencing technology that tags SNPs and haplotypes in gene-rich regions was used in previous genome-wide association studies (GWAS) for sodicity tolerance in bread wheat. Thirty-nine novel SNPs including 18 haplotypes for yield and yield-components were identified. The present study aimed at developing SNP-derived markers by precisely locating new SNPs on ~180 bp allelic sequence of t-GBS, marker validation, and SNP functional characterization based on its exonic location. We identified unknown locations of significant SNPs/haplotypes by aligning allelic sequences on to IWGSC RefSeqv1.0 on respective chromosomes. Eighteen out of the target 39 SNP locations …

Induction Of Ploidy Level On Three Patchouli (Pogostemon Cablin Benth) Cultivars By Colchicine In Vitro, Suseno Amien, Nikkita Putri Darmawan, Daniya Fathya

Jurnal Kultivasi

Patchouli plants produce essential oils and are used as a raw material and fixative agent for perfumes. The most widely cultivated patchouli plant in Indonesia is Aceh patchouli. Sexual propagation in Aceh patchouli plants is impossible as it does not flower. Genetic diversity can be elevated through another method, such as polyploidy induction using colchicine. This research aimed to evaluate the state-of-the-art application of polyploidization techniques as a breeding tool for callus-based explants. A completely randomized design with a factorial pattern was used in this experiment consisting of two factors, patchouli cultivars (Sidikalang, Tapak Tuan, and Lhokseumawe) and colchicine concentration …

Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott

Research in Biology

The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …

Testing Of Resistance To Downy Mildew On Several Sweet Corn Inbred Lines Of 7th Generation, Heri Kustanto

Jurnal Kultivasi

Sweet corn is one of the most well-known horticultural crop commodities and can adapt to various climates and environments. Efforts to control downy mildew disease continue to be carried out in collaboration with various parties in different ways, including genetic improvement of the varieties planted. The purpose of this study was to test the resistance of the 7th generation of sweet corn inbred lines to downy mildew and to find out the phenotypic characters that affect downy mildew. The new inbred lines obtained from the results of a long breeding process will be better tested for resistance to downy mildew …

Breeding Cotton For Determinate Growth Habit: Increasing Cotton Fiber Yield By Remobilization Of End-Of-Season Perennial Reserves, Salman Naveed

All Dissertations

Cotton (Gossypium spp.) is a major export commodity for the U.S. Despite advances in plant breeding and management practices, the cotton yield gains in the U.S. have stagnated. Therefore, to increase cotton fiber yield, we undertook a novel approach by altering the growth habit of cotton from perennial to annual. In this effort, eight genotypes showing strong expression alleles for five floral induction and meristem identity genes were selected from 44 Upland cotton mini-core collection genotypes and genetically crossed in various combinations to stack the strong expression alleles in a genotype. These efforts have allowed us to develop high-yielding, …

Identifying Genetic Sources Of Anthracnose Resistance In Global Sorghum Lines, Mary-Frances Behnke

All Theses

Anthracnose of sorghum (causal agent: Colletotrichum sublineola) is the most detrimental disease of sorghum (Sorghum bicolor) worldwide, significantly reducing grain yield. This study includes a literature review of the disease and a meta-analysis of genome-wide association studies (GWAS) of anthracnose-resistance in sorghum as means to elucidate genetic sources of the trait. The meta-analysis includes genotypic and phenotypic data from 5 unique studies and 1,071 sorghum lines. Original genotyping-by-sequencing data from were obtained primarily from National Center for Biotechnology Information’s Sequence Read Archive (NCBI-SRA) and prepared and processed using the Tassel 5 GBS v2. pipeline. Phenotypic data were …

B. R. Wells Arkansas Rice Research Studies 2022, J. Hardke, X. Sha, N. Bateman

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading rice producer in the United States. The state represents 50.1% of total U.S. rice production and 49.8% of the total acres planted to rice in 2022. Rice cultural practices vary across the state and across the U.S. However, these practices are also dynamic and continue to evolve in response to changing political, environmental, and economic times. This survey was initiated in 2002 to monitor and record changes in the way Arkansas rice producers approach their livelihood. The survey was conducted by polling county extension agents in each of the counties in Arkansas that produce rice. Questions …

Genetic Dissection Of Alkalinity Tolerance In Rice (Oryza Sativa L.), Lovepreet Singh

LSU Doctoral Dissertations

Alkaline stress is a major threat to rice production globally. The resources for genetic improvement of alkalinity tolerance in rice are limited. We performed genome-wide association study (GWAS) and QTL mapping integrated with whole genome sequencing and expression analysis to identify and confirm QTLs and candidate genes. We developed a near-isogenic line (NIL) population to fine map the two important QTLs. In GWAS experiment, we genotyped and phenotyped a panel of indica and japonica genotypes for alkalinity tolerance and identified genomic regions and candidate genes conferring alkalinity tolerance. Principal component analysis revealed that alkalinity tolerance score, shoot dry weight, and …

Genetic Resources And Precise Gene Editing For Targeted Improvement Of Barley Abiotic Stress Tolerance, Sakura Karunarathne, Esther Walker, Darshan Sharma, Chengdao Li, Yong Han

Grain and Other Field Crops Research Articles

Abiotic stresses, predominately drought, heat, salinity, cold, and waterlogging, adversely affect cereal crops. They limit barley production worldwide and cause huge economic losses. In barley, functional genes under various stresses have been identified over the years and genetic improvement to stress tolerance has taken a new turn with the introduction of modern gene-editing platforms. In particular, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is a robust and versatile tool for precise mutation creation and trait improvement. In this review, we highlight the stress-affected regions and the corresponding economic losses among the main barley producers. We collate about …

Likelihood-Based Tests Of Species Tree Hypotheses, Richard Adams, Michael Degiorgio

Entomology and Plant Pathology Faculty Publications and Presentations

Likelihood-based tests of phylogenetic trees are a foundation of modern systematics. Over the past decade, an enormous wealth and diversity of model-based approaches have been developed for phylogenetic inference of both gene trees and species trees. However, while many techniques exist for conducting formal likelihood-based tests of gene trees, such frameworks are comparatively underdeveloped and underutilized for testing species tree hypotheses. To date, widely used tests of tree topology are designed to assess the fit of classical models of molecular sequence data and individual gene trees and thus are not readily applicable to the problem of species tree inference. To …

Arkansas Corn And Grain Sorghum Research Studies 2022, Victor Ford, Jason Kelley, Nathan Mckinney Ii

Arkansas Agricultural Experiment Station Research Series

The 2022 edition of the Arkansas Corn and Grain Sorghum Research Studies Series includes research results on topics pertaining to corn and grain sorghum production, including weed, disease, and insect management; economics; sustainability; irrigation; post-harvest drying; soil fertility; mycotoxins; cover crop management; and research verification program results. Our objective is to capture and broadly distribute the results of research projects funded by the Arkansas Corn and Grain Sorghum Board. The intended audience includes producers and their advisors, current investigators, and future researchers. The Series serves as a citable archive of research results.

Reports in this publication are 2–3 year summaries. …

Development And Issues Of Biotech Seed Industry In China, Peijuan Chi, Hualing Xie, Ping Zhao, Fang Chen, Ning Wu, Zhixi Tian, Weicai Yang, Yanping Yang

Bulletin of Chinese Academy of Sciences (Chinese Version)

Biotech seed industry is a strategic core industry. Biotechnology combined with digital technology has promoted the seed industry into an intelligent era, and the breeding paradigm has changed from “experimental selection” to “computational selection”. Biotech seed industry has become a research and development intensive industry, and the market is highly concentrated, which is controlled by large multinational enterprises. The scientific and technological output of China and the United States is in the first echelon, and the number of papers and authorized patents ranks among the top two in the world. From the perspective of core competitiveness, the United States is …

Genomic Approaches To Enhance Adaptive Plasticity To Cope With Soil Constraints Amidst Climate Change In Wheat, Roopali Bhoite, Yong Han, Alamuru Krishna Chaitanya, Rajeev K. Varshney, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Climate change is varying the availability of resources, soil physicochemical properties, and rainfall events, which collectively determines soil physical and chemical properties. Soil constraints—acidity (pH < 6), salinity (pH ≤ 8.5), sodicity, and dispersion (pH > 8.5)—are major causes of wheat yield loss in arid and semiarid cropping systems. To cope with changing environments, plants employ adaptive strategies such as phenotypic plasticity, a key multifaceted trait, to promote shifts in phenotypes. Adaptive strategies for constrained soils are complex, determined by key functional traits and genotype × environment × management interactions. The understanding of the molecular basis of stress tolerance is particularly challenging for plasticity traits. Advances in sequencing and high-throughput genomics …

International Rice Outlook: International Rice Baseline Projections 2022-2032, A. Durand-Morat, S. Bairagi, W. Mulimbi

AAES Research Reports and Research Bulletins

Rice prices in Asia increased since the Summer of 2022 mainly due to worries about a reduction in rice production in India caused by an abnormal monsoon season (Fig. 1). The upward trend in export prices solidified after India implemented a 20% export tariff on brown and milled long-grain rice, and a complete ban on exports of broken rice, in September 2022 to curve down exports and release the pressure on domestic rice prices. India’s rice export prices increased since then and proportionally to the value of the export tax. The latest estimates put rice production at 128 million metric …

Fluidity And Inconstancy: Australian Bush Tomatoes As An Exemplar Of Non-Normative Sex Expression, Christopher T. Martine

Faculty Journal Articles

Solanum, a genus of ~1500 global species, is one of the more interesting plant groups in which to study reproductive biology and ecology. Overwhelmingly, species in this group express full cosexuality, where individual plants have flowers containing both fully-functioning “male” (staminate) and “female” (carpellate) organs. However, there have been multiple and widespread evolutionary transitions within the genus to non-normative variations on this ancestral condition. Australian bush tomatoes (ca. 40 species) are especially diverse in this regard, with uncommon variation and combinations of unisexuality and cosexuality -- including, most notably, two sexual systems known as dioecy (unisexual male or female …