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Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott 2023 Southern Adventist University

Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott

Research in Biology

The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …


Method Developments To Identify Loci And Selection Patterns Associated With Genotype By Environment Interactions In Soybean, Mary M. Happ 2023 University of Nebraska - Lincoln

Method Developments To Identify Loci And Selection Patterns Associated With Genotype By Environment Interactions In Soybean, Mary M. Happ

Theses, Dissertations, and Student Research in Agronomy and Horticulture

For many complex traits such as grain yield, genotype by environment (GxE) interactions are a prevalent source of phenotypic variation. Exploring the capacity of different methodologies to help describe and quantify the GxE interaction landscape for grain yield is an important step in informing plant breeders what the most viable strategies for management and exploitation of GxE may be. In this endeavor, we compared the results from multiple genome wide association studies (GWAS) that used either stability estimators as a phenotype to capture GxE variance, or directly mapped GxE in a mixed model for yield. Leading into this study, a …


The Structural Characterization And Bioactivity Assessment Of Nonspecific Lipid Transfer Protein 1 (Nsltp1) From Caraway (Carum Carvi) Seeds, Taibah Aldakhil, Saud O. Alshammari, Bushra Siraj, Bishoy El-Aarag, Shamshad Zarina, David Salehi, Aftab Ahmed 2023 Chapman University

The Structural Characterization And Bioactivity Assessment Of Nonspecific Lipid Transfer Protein 1 (Nsltp1) From Caraway (Carum Carvi) Seeds, Taibah Aldakhil, Saud O. Alshammari, Bushra Siraj, Bishoy El-Aarag, Shamshad Zarina, David Salehi, Aftab Ahmed

Pharmacy Faculty Articles and Research

Background

Carum carvi (caraway) of the Apiaceae family has been used in many cultures as a cooking spice and part of the folk medicine. Previous reports primarily focus on the medicinal properties of caraway seed essential oil and the whole seeds extract. However, no effort has been made to study caraway proteins and their potential pharmacological properties, including nonspecific lipid transfer protein (nsLTP), necessitating further research. The current study aimed to characterize nonspecific lipid transfer protein 1 (nsLTP1) from caraway seed, determine its three-dimensional structure, and analyze protein–ligand complex interactions through docking studies. We also evaluated nsLTP1 in vitro cytotoxic …


Molecular Identification Of Oomycete Species Associated With Woody Plants In Louisiana And Survey Of Oomycete Species Associated With Live Oak Trees Planted On The Louisiana State University Campus, Hamilton Crockett 2023 Louisiana State University and Agricultural and Mechanical College

Molecular Identification Of Oomycete Species Associated With Woody Plants In Louisiana And Survey Of Oomycete Species Associated With Live Oak Trees Planted On The Louisiana State University Campus, Hamilton Crockett

LSU Master's Theses

Three Oomycetes genera, Phytophthora, Phytopythium and Pythium, contain many important plant pathogens. Historically, Phytophthora infestans, responsible for the great Irish potato famine, and Phytophthora ramorum, causal agent of sudden oak death disease, are the two better-known high impact pathogens. The LSU AgCenter Plant Diagnostic Center (PDC) has been isolating putative Phytophthora and related species from combined root and soil samples collected from various woody ornamentals and trees for over a decade. In August of 2016, Louisiana received torrential rains, which resulted in historic flooding in several southern parishes. During subsequent years, the PDC staff isolated a …


Controlling Soil Erosion After Wildfire And Guiding Recovery In Southern Utah, Kristina Young, Kara Dohrenwend, Matt McEttrick, Henry Grover 2023 Rim to Rim Restoration

Controlling Soil Erosion After Wildfire And Guiding Recovery In Southern Utah, Kristina Young, Kara Dohrenwend, Matt Mcettrick, Henry Grover

All Current Publications

Wildfire is a natural part of many ecosystems in the Four Corners region (Southern Utah, Northern Arizona, Southwest Colorado, and Northwest New Mexico). However, after decades of fire suppression, the intensity and size of wildfires is increasing. This fact sheet is intended to help those in dry, monsoonal regions understand what happens to land after a wildfire and explains the processes of soil erosion. It outlines factors that influence how wildfire can affect soils and plants and provides suggestions for reducing soil erosion when necessary. The information presented is relevant to both private landowners and public lands managers whose landscapes …


Pathogen Emergence As Complex Biological Invasion: Lessons From Dynamical Systems Modeling, Sudam Surasinghe, Marisabel Rodriguez, Victor Meszaros, Jane Molofksy, Salvador Almagro-Moreno, Brandon Ogbunugafor 2023 Yale University

Pathogen Emergence As Complex Biological Invasion: Lessons From Dynamical Systems Modeling, Sudam Surasinghe, Marisabel Rodriguez, Victor Meszaros, Jane Molofksy, Salvador Almagro-Moreno, Brandon Ogbunugafor

Northeast Journal of Complex Systems (NEJCS)

Infectious disease emergence has become the target of cross-disciplinary efforts
that aim to understand and predict the shape of outbreaks. The many challenges
involved with the prediction of disease emergence events is a characteristic that in-
fectious diseases share with biological invasions in many subfields of ecology (e.g.,
how certain plants are able to successfully invade a new niche). Like infectious
diseases, biological invasions by plants and animals involve interactions between
agents (pathogens and plants in their respective cases) and a recipient niche. In
this study, we examine the problem of pathogen emergence through the lens of a
framework first …


Updating The Dual C And O Isotope—Gas-Exchange Model: A Concept To Understand Plant Responses To The Environment And Its Implications For Tree Rings, Rolf T. W. Siegwolf, Marco M. Lehmann, Gregory R. Goldsmith, Olga V. Churakova (Sidorova), Cathleen Mirande-Ney, Galina Timoveeva, Rosmarie B. Weigt, Matthias Saurer 2023 Swiss Federal Institute for Forest, Snow and Landscape Research WSL

Updating The Dual C And O Isotope—Gas-Exchange Model: A Concept To Understand Plant Responses To The Environment And Its Implications For Tree Rings, Rolf T. W. Siegwolf, Marco M. Lehmann, Gregory R. Goldsmith, Olga V. Churakova (Sidorova), Cathleen Mirande-Ney, Galina Timoveeva, Rosmarie B. Weigt, Matthias Saurer

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The combined study of carbon (C) and oxygen (O) isotopes in plant organic matter has emerged as a powerful tool for understanding plant functional responses to environmental change. The approach relies on established relationships between leaf gas exchange and isotopic fractionation to derive a series of model scenarios that can be used to infer changes in photosynthetic assimilation and stomatal conductance driven by changes in environmental parameters (CO2, water availability, air humidity, temperature, nutrients). We review the mechanistic basis for a conceptual model, in light of recently published research, and discuss where isotopic observations do not match our …


Do Cover Crop Mixtures Improve Soil Physical Health More Than Monocultures?, Humberto Blanco-Canqui 2023 University of Nebraska-Lincoln

Do Cover Crop Mixtures Improve Soil Physical Health More Than Monocultures?, Humberto Blanco-Canqui

Agronomy & Horticulture -- Faculty Publications

Rationale and Purpose — Adding multispecies cover crop (CC) mixtures could diversify the current simplified crop rotations and enhance soil health more than monoculture CCs. Further, CC mixtures with diverse plant species could adapt better to changing climatic and environmental conditions than monoculture CCs. However, our current understanding of the soil benefits of CC mixtures is still limited. This review discussed whether CC mixtures are better than monoculture CCs to improve soil physical health.

Methods — All studies published up to May 25, 2023, comparing soil physical properties between CC mixtures and their constituents grown as monocultures were searched in …


Exogenous Application Of Growth Regulators Improves The Growth And Quality Of Dahlia Variabilis L., Shahbaz Khan, Naeem Abbas, Zuhair Hasnain, Sami Ullah, Imran Hussain, Afroz Rais, Muhammad Atif Ghafoor, Danish Ibrar, Sohail Irshad, Maham Saddique, Muhammad Amjad Bashir, Samra Naseem, Muhammad Musawir Khan, Jawaher Alkahtani 2023 Colorado Water Centre, Colorado State University, Fort Collins, CO 80523, USA

Exogenous Application Of Growth Regulators Improves The Growth And Quality Of Dahlia Variabilis L., Shahbaz Khan, Naeem Abbas, Zuhair Hasnain, Sami Ullah, Imran Hussain, Afroz Rais, Muhammad Atif Ghafoor, Danish Ibrar, Sohail Irshad, Maham Saddique, Muhammad Amjad Bashir, Samra Naseem, Muhammad Musawir Khan, Jawaher Alkahtani

The Philippine Agricultural Scientist

Dahlia (Dahlia variabilis L.), a bulb crop sold as cut flower, garden ornamental/potted plant, becomes a popular cut flower in the world. This experiment was designed to study the effects of foliar applied gibberellic acid, zinc, ascorbic acid, and thiamine on the growth and flower attributes of dahlia. The study was conducted under greenhouse conditions at the Floriculture Research Area, University of Agriculture, Faisalabad, Pakistan. There were thirteen treatment with three levels each; gibberellic acid, zinc sulphate, ascorbic acid, and thiamine. First, foliar application was done at vegetative stage (after 45 d of transplantation) and second, at the flowering …


Impact Of Nitrogen Availability On The Accumulation Of Vegetative Lipids In Sorghum, Leticia Felicio Pasqualino 2023 University of Nebraska - Lincoln

Impact Of Nitrogen Availability On The Accumulation Of Vegetative Lipids In Sorghum, Leticia Felicio Pasqualino

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Sorghum (Sorghum bicolor (L.) Moench) is a versatile and prosperous feedstock crop for renewable energy production. With the increase in bioenergy demand, the production of higher oil content per biomass in a feedstock crop is a highly desirable trait for the biofuel conversion process yield. Especially if this outcome can be achieved using fewer inputs in the field, such as nitrogen. In microalgae species, nitrogen limitation has been associated with changes in the carbon storage metabolic pathway favoring triacylglycerol (TAG) accumulation. In this context, this study aimed to assess whether nitrogen starvation would result in a similar outcome in …


Characterization Of Boreal-Arctic Vegetation Growth Phases And Active Soil Layer Dynamics In The High-Latitudes Of North America: A Study Combining Multi-Year In Situ And Satellite-Based Observations, Michael G. Brown 2023 The Graduate Center, City University of New York

Characterization Of Boreal-Arctic Vegetation Growth Phases And Active Soil Layer Dynamics In The High-Latitudes Of North America: A Study Combining Multi-Year In Situ And Satellite-Based Observations, Michael G. Brown

Dissertations, Theses, and Capstone Projects

This dissertation examined the seasonal freeze/thaw activity in boreal-Arctic soils and vegetation physiology in Alaska, USA and Alberta, Canada, using in situ environmental measurements and passive microwave satellite observations. The boreal-Arctic high-latitudes have been experiencing ecosystem changes more rapidly in comparison to the rest of Earth due to the presently warming climatic conditions having a magnified effect over Polar Regions. Currently, the boreal-Arctic is a carbon sink; however, recent studies indicate a shift over the next century to become a carbon source. High-latitude vegetation and cold soil dynamics are influenced by climatic shifts and are largely responsible for the regions …


A Foundational Population Genetics Investigation Of The Sexual Systems Of Solanum (Solanaceae) In The Australian Monsoon Tropics Suggests Dioecious Taxa May Benefit From Increased Genetic Admixture Via Obligate Outcrossing, Jason T. Cantley, Ingrid E. Jordon-Thaden, Morgan Roche, Daniel S. Hayes, Stephamie Kate, Christopher T. Martine 2023 San Francisco State University

A Foundational Population Genetics Investigation Of The Sexual Systems Of Solanum (Solanaceae) In The Australian Monsoon Tropics Suggests Dioecious Taxa May Benefit From Increased Genetic Admixture Via Obligate Outcrossing, Jason T. Cantley, Ingrid E. Jordon-Thaden, Morgan Roche, Daniel S. Hayes, Stephamie Kate, Christopher T. Martine

Faculty Journal Articles

Solanum section Leptostemonum is an ideal lineage to test the theoretical framework regarding proposed evolutionary benefits of outcrossing sexual systems in comparison to cosexuality. Theoretically, non-cosexual taxa should support more genetic diversity within populations, experience less inbreeding, and have less genetic structure due to a restricted ability to self-fertilize. However, many confounding factors present challenges for a confident inference that inherent differences in sexual systems influence observed genetic patterns among populations. This study provides a foundational baseline of the population genetics of several species of different sexual systems with the aim of generating hypotheses of any factor—including sexual system—that influences …


Desalination: Adapting To A Changing Climate And An Increasing Demand For Freshwater, Rebecca A. Acosta 2023 University of San Francisco

Desalination: Adapting To A Changing Climate And An Increasing Demand For Freshwater, Rebecca A. Acosta

Master's Projects and Capstones

The state of California is just one place in the world that is experiencing an increasing demand for freshwater while also experiencing increasingly hotter conditions and longer periods of drought. There are a number of plants slated for development in the state of California but have been met with resistance from the public with concerns regarding their impacts to the surrounding marine environments. This paper provides background on desalination plants, provides evidence for a potential indicator species, identifies potential impacts to marine environments, and addresses stakeholder concerns and perceptions around desalination plants.


Establishing Big Sagebrush Seedlings On The Colorado Plateau, Kari E. Veblen, Eric Thacker, Mark Larese-Casanova, Kyle Nehring, Mike Duniway, Colby Brungard 2023 Utah State University

Establishing Big Sagebrush Seedlings On The Colorado Plateau, Kari E. Veblen, Eric Thacker, Mark Larese-Casanova, Kyle Nehring, Mike Duniway, Colby Brungard

All Current Publications

Factors such as soil type and precipitation vary across rangeland landscapes, and these factors affect restoration outcomes and ultimately mean that “one size fits all” management strategies are not effective across large, complex landscapes. Big sagebrush (Artemisia tridentata) is a foundational rangeland species that is important to wildlife habitat across the western U.S. On the Colorado Plateau, sagebrush is important browse for ungulates, such as mule deer and pronghorn, which motivates a great deal of restoration effort. However, most scientific knowledge of big sagebrush comes from the Great Basin, and we know much less about how to restore sagebrush on …


Evaluating Planting Green And Herbicides For Integrated Weed Management And Their Effect On Soil Properties In Corn And Soybean In Nebraska, Trey Stephens 2023 University of Nebraska-Lincoln

Evaluating Planting Green And Herbicides For Integrated Weed Management And Their Effect On Soil Properties In Corn And Soybean In Nebraska, Trey Stephens

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Producers across the Midwest are finding new ways to implement cover crops into cropping systems and the practice of “Planting Green” is one of the newest uses of cover crops. When planting green, producers plant their row crops into actively growing cover crops and terminate the cover crop at time of planting or shortly after planting. This practice would allow for higher biomass accumulation of the cover crop and could aid in weed management of herbicide-resistant weeds. The objective of the first two studies was to evaluate planting green and its effect on soil-applied residual herbicides, weed management, dicamba/glyphosate-resistant soybean …


Genomic Selection For Yield And Seed Composition Stability In An Applied Soybean Breeding Program, Benjamin Harms 2023 University of Nebraska - Lincoln

Genomic Selection For Yield And Seed Composition Stability In An Applied Soybean Breeding Program, Benjamin Harms

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Stability traits are of primary importance in plant breeding to ensure consistency in phenotype across a range of environments. However, selection efficiency and accuracy for stability traits can be hindered due to the requirement of obtaining phenotype data across multiple years and environments for proper stability analysis. Genomic selection is a method that allows prediction of a phenotype prior to observation in the field using genome-wide marker data and phenotype data from a training population. To assess prediction of stability traits, two elite-yielding soybean populations developed three years apart in the same breeding program were used. The individuals in each …


Biotic And Abiotic Factors Contributing To The Invasion Success Of Microstegium Vimineum In Eastern Deciduous Forests., Kimberly Anne Koenig 2023 University of Louisville

Biotic And Abiotic Factors Contributing To The Invasion Success Of Microstegium Vimineum In Eastern Deciduous Forests., Kimberly Anne Koenig

Electronic Theses and Dissertations

Forest managers face multiple challenges in maintaining woodland systems, including climate change, habitat destruction and fragmentation, and the invasion of novel species. Invaders can change microclimates, alter nutrient cycling and understory habitat, and outcompete native species, leading to native species population declines and reduction in species richness. To effectively combat plant invasions it is necessary to first understand factors contributing to invasive species spread, including the complex interactions between invaders and native biota. This dissertation investigates the abiotic conditions and biotic interactions associated with invasion success of the non-native grass Microstegium vimineum, which is a species of great concern …


A Programmatic Geographic Information Systems Analysis Of Plant Hardiness Zones, Andrew Bowen 2023 East Tennessee State University

A Programmatic Geographic Information Systems Analysis Of Plant Hardiness Zones, Andrew Bowen

Electronic Theses and Dissertations

The Plant Hardiness Zone Map consists of thirteen geographical zones that describe whether a plant can survive based on average annual minimal temperatures. As climate change progresses, minimum temperatures in all regions are expected to change. This work programmatically evaluates predicted future climate projection data and converts it to United States Department of Agriculture-defined hardiness zones. Through the next 80 years, hardiness zones are projected to move poleward; in effect, colder zones will lose area and warmer zones will gain area globally. Some implications include changes in crop growing degree days, which could alter crop productivity, migration and settlement of …


Plasticity Of Sorghum Biomass And Inflorescence Traits In Response To Nitrogen Application, Kyle M. Linders 2023 University of Nebraska-Lincoln

Plasticity Of Sorghum Biomass And Inflorescence Traits In Response To Nitrogen Application, Kyle M. Linders

Theses, Dissertations, and Student Research in Agronomy and Horticulture

Nitrogen is an essential nutrient required for growth and development in plants. Insufficient nitrogen availability can reduce vegetative growth and grain yield. However, nitrogen is a costly input for farmers, is energy intensive to manufacture, and runoff of excess nitrogen fertilizer impacts water quality. Compared to its close relative, maize, sorghum has much greater resilience to nitrogen and water deficit, and heat stress, allowing sorghum to be grown with fewer inputs and on marginal land. Variation in total biomass accumulation and grain yield between sorghum accessions, as well as between nitrogen conditions, can be largely explained by differences in vegetative …


Characterization Of Physical And Biochemical Traits In Wheat And Corn Plants Using High Throughput Image Analysis, Kantilata Thapa 2023 University of Nebraska-Lincoln

Characterization Of Physical And Biochemical Traits In Wheat And Corn Plants Using High Throughput Image Analysis, Kantilata Thapa

Biological Systems Engineering--Dissertations, Theses, and Student Research

Plant phenotyping has been recognized as a rapidly growing field of research due to the labor-intensive, destructive, and time-consuming nature of traditional phenotyping methods. These phenotyping bottlenecks can be addressed by advancements in image-based phenotyping like RGB and hyperspectral imaging for the assessment of plant traits important for breeding purposes. This study aims (1) to characterize the physical and biochemical traits of wheat and corn plants using RGB and hyperspectral imaging in the greenhouse, and (2) to estimate leaf nitrogen (N), phosphorus (P), and potassium (K) content using hyperspectral imaging and an analytical spectral device (ASD spectrometer) and compare the …


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