Other Plant Sciences Commons^™

A Global-Temporal Analysis On Phytophthora Sojae Resistance-Gene Efficacy, Austin G. Mccoy, Richard R. Belanger, Carl A. Bradley, Daniel G. Cerritos-Garcia, Vinicius C. Garnica, Loren Giesler, Pablo E. Grijalba, Eduardo Guillin, Maria A. Henriquez, Yong Min Kim, Dean K. Malvick, Rashelle L. Matthiesen, Santiago X. Mideros, Zachary A. Noel, Alison E. Robertson, Mitchell G. Roth, Clarice L. Schmidt, Damon L. Smith, Adam H. Sparks, Darcy E.P. Telenko, Vanessa Tremblay, Owen Wally, Martin I. Chilvers

Papers in Plant Pathology

Plant disease resistance genes are widely used in agriculture to reduce disease outbreaks and epidemics and ensure global food security. In soybean, Rps (Resistance to Phytophthora sojae) genes are used to manage Phytophthora sojae, a major oomycete pathogen that causes Phytophthora stem and root rot (PRR) worldwide. This study aims to identify temporal changes in P. sojae pathotype complexity, diversity, and Rps gene efficacy. Pathotype data was collected from 5121 isolates of P. sojae, derived from 29 surveys conducted between 1990 and 2019 across the United States, Argentina, Canada, and China. This systematic review shows a loss of efficacy of …

Multiple Light Signaling Pathways Control Solar Tracking In Sunflowers, Christopher J. Brooks, Hagop S. Atamian, Stacey L. Harmer

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Sunflowers are famous for their ability to track the sun throughout the day and then reorient at night to face east the following morning. This occurs by differential growth patterns, with the east sides of stems growing more during the day and the west sides of stems growing more at night. This process, termed heliotropism, is generally believed to be a specialized form of phototropism; however, the underlying mechanism is unknown. To better understand heliotropism, we compared gene expression patterns in plants undergoing phototropism in a controlled environment and in plants initiating and maintaining heliotropic growth in the field. We …

Lichens Of Iller Creek: A Checklist For The Iller Creek Unit, A Division Of Dishman Hills Conservation Area, Spokane Valley, Wa, Devin M. Mumey, Giovanna Bishop, Jessica L. Allen

2023 Symposium

The field of biodiversity documentation encompasses a broad range of research including new species discovery and description, compilation of species present in a given area, and investigation of interspecies interaction. In an era of increasingly devastating and rapid environmental change, documenting biodiversity has become increasingly important. Anthropogenic effects on urban-adjacent natural areas are especially significant, as they can cause numerous, often drastic, responses in ecosystems. Our objective here was to document the lichen biodiversity in a large urban-adjacent protected area: the Iller Creek Unit of the Dishman Hills Conservation Area in Spokane Valley, Washington. This unit encompasses a diversity of …

Development And Characterization Of Rice Lines Carrying Both Sub1 And Anaerobic Germination Tolerance: Sub1a Does Not Inhibit Ag [Research Note], Georgina V. Vergara, Abdelbagi M. Ismail

The Philippine Agricultural Scientist

Accelerated coleoptile elongation in flooded soils allowed seedling shoots to reach the water surface to maintain gas exchange and CO₂ fixation. Rice genotypes with tolerance of flooding during germination (anaerobic germination, AG) exhibited fast coleoptile elongation as an escape mechanism. On the other hand, tolerance of complete submergence during the vegetative stage, conferred by SUB1A, involved growth retardation to conserve energy for maintenance metabolism, with resumption of growth upon de-submergence. Combining genes controlling flood tolerance at these two stages with contrasting mechanisms was necessary in flood-prone areas for protection during germination under direct seeding and during vegetative stage. …

Dicamba Off-Target Movement From Applications On Soybeans At Two Growth Stages, Greg R. Kruger, Guilherme S. Alves, Kasey Schroeder, Jeffrey A. Golus, Daniel B. Reynolds, Darrin M. Dodds, Ashli E. Brown, Bradley K. Fritz, Wesley C. Hoffmann

Agronomy and Horticulture: Faculty Publications

The objective of this study was to evaluate dicamba off-target movement during and after applications over soybean at two growth stages. Dicamba-tolerant soybean [Glycine max (L.) Merr.] at V3 and R1 growth stages in Nebraska and Mississippi fields were treated with diglycolamine salt of dicamba (560 g ae ha⁻¹), potassium salt of glyphosate (1260 g ae ha⁻¹), and a drift-reducing adjuvant (0.5% v v⁻¹). Filter papers positioned outside the sprayed area were used to determine primary movement and air samplers positioned at the center of sprayed area were used to calculate dicamba flux …

Climate Interactions Drive Tree Physiology And Growth In A Northeastern Forest Ecotone, Alexandra M. Barry

Electronic Theses and Dissertations

Climate change is a threat to global forest ecosystems. In the northeastern United States, forest trees are facing rising temperatures and increasingly inconsistent moisture regimes. In addition to long-term changes in climate conditions, there is concern about the potential for more frequent and intense climate extremes, which can have severe and rapid negative effects on tree physiology and growth. Further, climate extremes may co-occur to produce a greater magnitude of effect than the sum of their parts, with a prominent example being hot droughts, which are increasing in occurrence and severity. The impact of these and other extreme climate interactions …

Long-Term Influence Of Commercial Thinning On Spruce-Fir Forests, Bishnu H. Wagle Mr.

Electronic Theses and Dissertations

This study investigates long-term influences of pre-commercial thinning (PCT) and commercial thinning (CT) treatments in spruce-fir forests, a key forest type in northeastern North America. Utilizing repeated measurements from replicated experimental research sites (n=15) in Maine, both stand- and tree-level responses to contrasting CT with and without prior PCT (NoPCT) have been quantified. In addition, an in-depth economic analysis, exploring the impact of different thinning treatments, associated costs, merchantability specifications, varying timber and carbon prices, and discount rates, on net present value (NPV) has been carried out using both measured and projected data. Thinning treatments at nine PCT sites combined …

Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott

Research in Biology

The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …

Effect Of Cutting Management On The Forage Production And Quality Of Tepary Bean (Phaseolus Acutifolius A. Gray), Travis W. Witt, Brian K. Northup, Timothy G. Porch, Santos Barrera, Carlos A. Urrea

Agronomy and Horticulture: Faculty Publications

Tepary bean (Phaseolus acutifolius A. Gray) is an underutilized drought tolerant annual legume, originating from the Sonoran Desert, that may be a beneficial forage/hay for beef cattle in the Southern Great Plains of the US (SGP). The SGP has erratic rainfall and periods of intermittent drought exacerbated by high summer temperatures. In 2020 and 2021, a split-plot design was used to evaluate 13 genotypes of tepary bean and a forage soybean (control) at El Reno, OK, USA to compare production of plant biomass and forage nutritive value parameters under seven harvest regimes. Genotypes were used as the main plot …

Lunchbox Hydroponics, Stacy A. Adams

Agronomy and Horticulture: Faculty Publications

Growing plants with hydroponics is less complicated than it may appear when the fundamentals of the system are understood. This publication provides instruction on the construction of a simple hydroponic prototype suitable for school or youth education programs. The fundamentals of hydroponics, basic design of a solution system, seed starting, creating a nutrient solution, and plant care are explored through this engaging project. This prototype can successfully be used to grow small edible and herb plants in the classroom or at home.

Method Developments To Identify Loci And Selection Patterns Associated With Genotype By Environment Interactions In Soybean, Mary M. Happ

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

For many complex traits such as grain yield, genotype by environment (GxE) interactions are a prevalent source of phenotypic variation. Exploring the capacity of different methodologies to help describe and quantify the GxE interaction landscape for grain yield is an important step in informing plant breeders what the most viable strategies for management and exploitation of GxE may be. In this endeavor, we compared the results from multiple genome wide association studies (GWAS) that used either stability estimators as a phenotype to capture GxE variance, or directly mapped GxE in a mixed model for yield. Leading into this study, a …

Increasing Rye Cover Crop Biomass Production After Corn Residue Removal To Balance Economics And Soil Health, Sabrina J. Ruis, Humberto Blanco-Canqui, Paul J. Jasa, Glen Slater, Richard B. Ferguson

Agronomy and Horticulture: Faculty Publications

Low or variable cover crop (CC) biomass production could limit CC benefits. Longer CC growing periods via late termination could increase CC benefits, especially under limited crop residue return. We studied whether early (2–3 wk before planting)- or late (at planting)-terminated winter rye (Secale cereale L.) CC maintains soil properties, crop yields, and farm income under 0%, 25%, 50%, 75%, and 100% corn (Zea mays L.) residue removal in rainfed and irrigated no-till in the U.S. Great Plains after 6 yr. Early-terminated CCs produced < 1 Mg ha^-1 of biomass while late-terminated CCs averaged 1.6 Mg ha^-1 at the rainfed …

Soybean Tolerance To Ultra-Low Doses Of Dicamba: Hormesis Or Not, Luka Milosevic, O. Adewale Osipitan, Jon E. Scott, Stevan Z. Knezevic

Agronomy and Horticulture: Faculty Publications

The widespread use of dicamba across the soybean growing regions of the United States resulted in increased off-target movement of this herbicide to susceptible vegetations. There are speculations that a drift of sublethal (ultra-low) doses of dicamba can enhance soybean growth and yield through a phenomenon called hormesis. Field studies were conducted during 2018 and 2019 to determine whether soybean growth and yield can be enhanced with ultra-low doses (0.0112–56 g ae ha^-1) of dicamba, applied at three growth stages (V2, R1 and R2). There was no evidence that the ultra-low doses of dicamba (0.0112–56 g ae ha …

The Structural Characterization And Bioactivity Assessment Of Nonspecific Lipid Transfer Protein 1 (Nsltp1) From Caraway (Carum Carvi) Seeds, Taibah Aldakhil, Saud O. Alshammari, Bushra Siraj, Bishoy El-Aarag, Shamshad Zarina, David Salehi, Aftab Ahmed

Pharmacy Faculty Articles and Research

Background

Carum carvi (caraway) of the Apiaceae family has been used in many cultures as a cooking spice and part of the folk medicine. Previous reports primarily focus on the medicinal properties of caraway seed essential oil and the whole seeds extract. However, no effort has been made to study caraway proteins and their potential pharmacological properties, including nonspecific lipid transfer protein (nsLTP), necessitating further research. The current study aimed to characterize nonspecific lipid transfer protein 1 (nsLTP1) from caraway seed, determine its three-dimensional structure, and analyze protein–ligand complex interactions through docking studies. We also evaluated nsLTP1 in vitro cytotoxic …

Genomes To Fields 2022 Maize Genotype By Environment Prediction Competition, Dayane Cristina Lima, Jacob D. Washburn, José Ignacio Varela, Qiuyue Chen, Joseph L. Gage, Maria Cinta Romay, James Holland, David Ertl, Marco Lopez-Cruz, Fernando M. Aguate, Gustavo De Los Campos, Shawn Kaeppler, Timothy Beissinger, Martin Bohn, Edward Buckler, Jode Edwards, Sherry Flint-Garcia, Michael A. Gore, Candice N. Hirsch, Joseph E. Knoll, John Mckay, Richard Minyo, Seth C. Murray, Osler A. Ortez, James C. Schnable, Rajandeep S. Sekhon, Maninder P. Singh, Erin E. Sparks, Addie Thompson, Mitchell Tuinstra, Jason Wallace, Teclemariam Weldekidan, Wenwei Xu, Natalia De Leon

Agronomy and Horticulture: Faculty Publications

Objectives The Genomes to Fields (G2F) 2022 Maize Genotype by Environment (GxE) Prediction Competition aimed to develop models for predicting grain yield for the 2022 Maize GxE project field trials, leveraging the datasets previously generated by this project and other publicly available data.

Data description This resource used data from the Maize GxE project within the G2F Initiative [1]. The dataset included phenotypic and genotypic data of the hybrids evaluated in 45 locations from 2014 to 2022. Also, soil, weather, environmental covariates data and metadata information for all environments (combination of year and location). Competitors also had access to ReadMe …

Role Of Biotechnology In Creating Sustainable Agriculture, Saurav Das, Manjit Kumar Ray, Dinesh Panday, Piyush Kumar Mishra

Agronomy and Horticulture: Faculty Publications

This narrative review paper discusses the role of biotechnology in the development of sustainable agriculture. The paper begins by defning sustanability and highlights the importance of biotechnology in establishing sustainable agriculture. Sustainable agriculture is an approach that prioritizes meeting current food and fiber production needs while conserving and enhancing natural resources for future generations. To achieve agricultural sustainability, it is necessary to strike a balance between economic viability, environmental stewardship, and social responsibility. This can be difficult, especially in the face of biotic and abiotic stresses such as pests, diseases, climate change, soil degradation, and water depletion. The prevalence of …

A Protein Kinase Coordinates Cycles Of Autophagy And Glutaminolysis In Invasive Hyphae Of The Fungus Magnaporthe Oryzae Within Rice Cells, Gang Li, Ziwen Gong, Nawaraj Dulal, Margarita Marroquin-Guzman, Raquel O. Rocha, Michael Richter, Richard Wilson

Papers in Plant Pathology

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these …

A Protein Kinase Coordinates Cycles Of Autophagy And Glutaminolysis In Invasive Hyphae Of The Fungus Magnaporthe Oryzae Within Rice Cells, Gang Li, Ziwen Gong, Nawaraj Dulal, Margarita Marroquin-Guzman, Raquel O. Rocha, Michael Richter, Richard Wilson

Papers in Plant Pathology

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these …

A Protein Kinase Coordinates Cycles Of Autophagy And Glutaminolysis In Invasive Hyphae Of The Fungus Magnaporthe Oryzae Within Rice Cells, Gang Li, Ziwen Gong, Nawaraj Dulal, Margarita Marroquin-Guzman, Raquel O. Rocha, Michael Richter, Richard A. Wilson

Papers in Plant Pathology

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these …

Molecular Identification Of Oomycete Species Associated With Woody Plants In Louisiana And Survey Of Oomycete Species Associated With Live Oak Trees Planted On The Louisiana State University Campus, Hamilton Crockett

LSU Master's Theses

Three Oomycetes genera, Phytophthora, Phytopythium and Pythium, contain many important plant pathogens. Historically, Phytophthora infestans, responsible for the great Irish potato famine, and Phytophthora ramorum, causal agent of sudden oak death disease, are the two better-known high impact pathogens. The LSU AgCenter Plant Diagnostic Center (PDC) has been isolating putative Phytophthora and related species from combined root and soil samples collected from various woody ornamentals and trees for over a decade. In August of 2016, Louisiana received torrential rains, which resulted in historic flooding in several southern parishes. During subsequent years, the PDC staff isolated a …