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A Role For Heritable Transcriptomic Variation In Maize Adaptation To Temperate Environments, Guangchao Sun, Huihui Yu, Peng Wang, Martha Lopez‑Guerrero, Ravi V. Mural, Olivier N. Mizero, Marcin Grzybowski, Baoxing Song, Karin V. van Dijk, Daniel P. Schachtman, Chi Zhang, James C. Schnable 2023 University of Nebraska-Lincoln

A Role For Heritable Transcriptomic Variation In Maize Adaptation To Temperate Environments, Guangchao Sun, Huihui Yu, Peng Wang, Martha Lopez‑Guerrero, Ravi V. Mural, Olivier N. Mizero, Marcin Grzybowski, Baoxing Song, Karin V. Van Dijk, Daniel P. Schachtman, Chi Zhang, James C. Schnable

Agronomy & Horticulture -- Faculty Publications

Background: Transcription bridges genetic information and phenotypes. Here, we evaluated how changes in transcriptional regulation enable maize (Zea mays), a crop originally domesticated in the tropics, to adapt to temperate environments.

Result: We generated 572 unique RNA-seq datasets from the roots of 340 maize genotypes. Genes involved in core processes such as cell division, chromosome organization and cytoskeleton organization showed lower heritability of gene expression, while genes involved in anti-oxidation activity exhibited higher expression heritability. An expression genome-wide association study (eGWAS) identified 19,602 expression quantitative trait loci (eQTLs) associated with the expression of 11,444 genes. A …


Increased Light Exposure Decreases Lichen Abundance On Linden Trees In The Prairie Grassland Biome Of West Central Minnesota, Cameron Berthiaume, Sanjana Kidambi, Madelyn Schoenberger 2023 University of Minnesota - Morris

Increased Light Exposure Decreases Lichen Abundance On Linden Trees In The Prairie Grassland Biome Of West Central Minnesota, Cameron Berthiaume, Sanjana Kidambi, Madelyn Schoenberger

Scholarly Horizons: University of Minnesota, Morris Undergraduate Journal

Percent lichen cover on the four cardinal faces of linden trees on the University of Minnesota Morris campus was recorded in order to determine if lichen abundance is linked to the varying levels of sunlight each cardinal face receives.


Soybean Seed Protein Concentration Is Limited By Nitrogen Supply In Tropical And Subtropical Environments In Brazil, E. H. Figueiredo Moura da Silva, N. Cafaro La Menza, G. G. Munareto, A. J. Zanon, Santos Carvalho, F. R. Marin 2023 University of São Paulo

Soybean Seed Protein Concentration Is Limited By Nitrogen Supply In Tropical And Subtropical Environments In Brazil, E. H. Figueiredo Moura Da Silva, N. Cafaro La Menza, G. G. Munareto, A. J. Zanon, Santos Carvalho, F. R. Marin

Agronomy & Horticulture -- Faculty Publications

Soybean production contributes to ca. 60% of global plant-based protein used for food and feed. Brazil is the largest soybean producer and exporter, with 60% from tropical and 40% from subtropical environments. Nitrogen (N) can play an essential role in the storage of proteins in seeds; thus, it could be a key factor in increasing the quantity and quality of seeds in high-yielding soybean crops. Unlike in temperate environments, there is a gap of knowledge on whether soybean grown under tropical and subtropical climates are limited by N-fertilization to sustain the seed yield increase without detriments in seed protein concentration. …


An Innovative Approach To Improve Oil Production And Quality Of Mustard (Brassica Juncea L.) With Multi-Nutrient-Rich Polyhalite, Biswajit Pramanick, B.S. Mahapatra, Debarati Datta, Prithwiraj Dey, S.P. Singh, Ajay Kumar, Bappa Paramanik, Neeraj Awasthi 2023 University of Nebraska-Lincoln, Govind Ballabh Pant University of Agricultural & Technology, Dr. Rajendra Prasad Central Agricultural University

An Innovative Approach To Improve Oil Production And Quality Of Mustard (Brassica Juncea L.) With Multi-Nutrient-Rich Polyhalite, Biswajit Pramanick, B.S. Mahapatra, Debarati Datta, Prithwiraj Dey, S.P. Singh, Ajay Kumar, Bappa Paramanik, Neeraj Awasthi

Agronomy & Horticulture -- Faculty Publications

Polyhalite popularly known as POLY4 is a multi-nutrient fertiliser containing K, S, Mg, Ca, and micronutrients. POLY4 has a low carbon footprint, is certified for organic agriculture, and has the potential to improve crop productivity and quality attributes Indian mustard which often faces challenges due to imbalanced nutrition supplied in the current fertilisation schedule. The hypothesis of the study was that the multi-nutrient fartiliser POLY4 can ensure balanced nutrition for Indian mustard. Considering this, a field experiment was conducted during the winter seasons of 2017–18 and 2018–19 to evaluate the effect of POLY4 on Indian mustard (Brassica juncea L.) …


Pearl Millet Response To Drought: A Review, Nikee Shrestha, Hao Hu, Kumar Shrestha, Andrew N. Doust 2023 Oklahoma State University, University of Nebraska-Lincoln

Pearl Millet Response To Drought: A Review, Nikee Shrestha, Hao Hu, Kumar Shrestha, Andrew N. Doust

Agronomy & Horticulture -- Faculty Publications

The C4 grass pearl millet is one of the most drought tolerant cereals and is primarily grown in marginal areas where annual rainfall is low and intermittent. It was domesticated in sub-Saharan Africa, and several studies have found that it uses a combination of morphological and physiological traits to successfully resist drought. This review explores the short term and long-term responses of pearl millet that enables it to either tolerate, avoid, escape, or recover from drought stress. The response to short term drought reveals fine tuning of osmotic adjustment, stomatal conductance, and ROS scavenging ability, along with ABA and ethylene …


Pearl Millet Response To Drought: A Review, Nikee Shrestha, Hao Hu, Kumar Shrestha, Andrew N. Doust 2023 Oklahoma State University, University of Nebraska-Lincoln

Pearl Millet Response To Drought: A Review, Nikee Shrestha, Hao Hu, Kumar Shrestha, Andrew N. Doust

Agronomy & Horticulture -- Faculty Publications

The C4 grass pearl millet is one of the most drought tolerant cereals and is primarily grown in marginal areas where annual rainfall is low and intermittent. It was domesticated in sub-Saharan Africa, and several studies have found that it uses a combination of morphological and physiological traits to successfully resist drought. This review explores the short term and long-term responses of pearl millet that enables it to either tolerate, avoid, escape, or recover from drought stress. The response to short term drought reveals fine tuning of osmotic adjustment, stomatal conductance, and ROS scavenging ability, along with ABA and ethylene …


Screening Spring Wheat Genotypes For Tadreb-B1 And Fehw3 Genes Under Severe Drought Stress At The Germination Stage Using Kasp Technology, Elsayed A. Mohamed, Asmaa A. M. Ahmed, Matías Schierenbeck, Mohamed Y. Hussein, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam 2023 Assiut University

Screening Spring Wheat Genotypes For Tadreb-B1 And Fehw3 Genes Under Severe Drought Stress At The Germination Stage Using Kasp Technology, Elsayed A. Mohamed, Asmaa A. M. Ahmed, Matías Schierenbeck, Mohamed Y. Hussein, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam

Agronomy & Horticulture -- Faculty Publications

Drought stress is a major yield-limiting factor throughout the world in wheat (Triticum aestivum L.), causing losses of up to 80% of the total yield. The identification of factors affecting drought stress tolerance in the seedling stage is especially important to increase adaptation and accelerate the grain yield potential. In the current study, 41 spring wheat genotypes were tested for their tolerance to drought at the germination stage under two different polyethylene glycol concentrations (PEG) of 25% and 30%. For this purpose, twenty seedlings from each genotype were evaluated in triplicate with a randomized complete block design (RCBD) in …


Evaluation Of Pollination Traits Important For Hybrid Wheat Development In Great Plains Germplasm, Nicholas Garst, Vikas Belamkar, Amanda Easterly, Mary J. Guttieri, Hannah Stoll, Amir M. H. Ibrahim, P. Stephen Baenziger 2023 University of Nebraska-Lincoln

Evaluation Of Pollination Traits Important For Hybrid Wheat Development In Great Plains Germplasm, Nicholas Garst, Vikas Belamkar, Amanda Easterly, Mary J. Guttieri, Hannah Stoll, Amir M. H. Ibrahim, P. Stephen Baenziger

Agronomy & Horticulture -- Faculty Publications

Hybrid wheat (Triticum aestivumL.) offers potential yield advantages over conventional inbred cultivars. For hybrid wheat to be a commercial success, the cost to produce the hybrid seed needs to be minimized. Although wheat is naturally selfpollinated, hybrid wheat seed production can be improved by increasing the amount and availability of pollen for cross-pollination. This research examined 19 pollination traits using the Hard Winter Wheat Association Mapping Panel for 3 years. Anther extrusion, pollen 50 date (date at which a genotype has 50% of spikes pollinating), plant height, and pollination duration (last spike pollen 50 date minus first spike …


Screening Spring Wheat Genotypes For Tadreb-B1 And Fehw3 Genes Under Severe Drought Stress At The Germination Stage Using Kasp Technology, Elsayed A. Elsayed, Asmaa A. M. Ahmed, Matías Schierenbeck, Schierenbeck Y. Hussein, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam 2023 Assiut University

Screening Spring Wheat Genotypes For Tadreb-B1 And Fehw3 Genes Under Severe Drought Stress At The Germination Stage Using Kasp Technology, Elsayed A. Elsayed, Asmaa A. M. Ahmed, Matías Schierenbeck, Schierenbeck Y. Hussein, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam

Agronomy & Horticulture -- Faculty Publications

Drought stress is a major yield-limiting factor throughout the world in wheat (Triticum aestivum L.), causing losses of up to 80% of the total yield. The identification of factors affecting drought stress tolerance in the seedling stage is especially important to increase adaptation and accelerate the grain yield potential. In the current study, 41 spring wheat genotypes were tested for their tolerance to drought at the germination stage under two different polyethylene glycol concentrations (PEG) of 25% and 30%. For this purpose, twenty seedlings from each genotype were evaluated in triplicate with a randomized complete block design (RCBD) in …


Long-Term Manure Application Improves Soil Health And Stabilizes Carbon In Continuous Maize Production System, Saurav Das, Daniel Liptzin, Bijesh Maharjan 2023 University of Nebraska - Lincoln

Long-Term Manure Application Improves Soil Health And Stabilizes Carbon In Continuous Maize Production System, Saurav Das, Daniel Liptzin, Bijesh Maharjan

Agronomy & Horticulture -- Faculty Publications

Soil health lies at the core of a sustainable food production system. A comprehensive evaluation of different agronomic practices and their effect on soil health is essential to determine the best practices that support soil ecosystem services. However, it may take years or decades to observe measurable changes in soil health under varying management practices. The objective of this experiment was to evaluate the effects of long-term (>77 years) manure and inorganic nitrogen (N) fertilizer on soil health and determine the interrelationship among the measured soil bio-physicochemical indicators. The study also aims to understand the sustainability of the monocropping …


On Correlation Between Canopy Vegetation And Growth Indexes Of Maize Varieties With Different Nitrogen Efficiencies, Xia Zhao, ShuaiLi Wang, Tao Wen, Jiamin Xu, Bao Huang, Shufeng Yan, Gangqiang Gao, Yali Zhao, Hongping Li, Jiangfang Qiao, Jinliang Yang, Lianhai Wu, Hongwei Wang, Tianxue Liu, Xinyuan Mu 2023 Henan Academy of Agricultural Sciences/Henan International Joint Laboratory on Maize Precision Production

On Correlation Between Canopy Vegetation And Growth Indexes Of Maize Varieties With Different Nitrogen Efficiencies, Xia Zhao, Shuaili Wang, Tao Wen, Jiamin Xu, Bao Huang, Shufeng Yan, Gangqiang Gao, Yali Zhao, Hongping Li, Jiangfang Qiao, Jinliang Yang, Lianhai Wu, Hongwei Wang, Tianxue Liu, Xinyuan Mu

Agronomy & Horticulture -- Faculty Publications

Studying the canopy spectral reflection characteristics of different N-efficient maize varieties and analyzing the relationship between their growth indicators and spectral vegetation indices can help the breeding and application of N-efficient maize varieties. To achieve the optimal management of N fertilizer resources, developing N-efficient maize varieties is necessary. In this research, maize varieties, i.e., the low-N-efficient (Zhengdan 958, ZD958), the high-N efficient (Xianyu 335, XY335), the double-high varieties (Qiule 368, QL368), and the double inefficient-type varieties (Yudan 606 YD606), were used as materials. Results indicate that nitrogen fertilization significantly increased the vegetation indices NDVI, GNDVI, GOSAVI, and RVI of maize …


The Intertwining Of Zn-Finger Motifs And Abiotic Stress Tolerance In Plants: Current Status And Future Prospects, Debojyoti Moulick, Karma Landup Bhutia, Sukamal Sarkar, Anirban Roy, Udit Nandan Mishra, Biswajit Pramanick, Sagar Maitra, Tanmoy Shankar, Swati Hazra, Milan Skalicky, Marian Brestic, Viliam Barek, Akbar Hossain 2023 University of Kalyani

The Intertwining Of Zn-Finger Motifs And Abiotic Stress Tolerance In Plants: Current Status And Future Prospects, Debojyoti Moulick, Karma Landup Bhutia, Sukamal Sarkar, Anirban Roy, Udit Nandan Mishra, Biswajit Pramanick, Sagar Maitra, Tanmoy Shankar, Swati Hazra, Milan Skalicky, Marian Brestic, Viliam Barek, Akbar Hossain

Agronomy & Horticulture -- Faculty Publications

Environmental stresses such as drought, high salinity, and low temperature can adversely modulate the field crop’s ability by altering the morphological, physiological, and biochemical processes of the plants. It is estimated that about 50% + of the productivity of several crops is limited due to various types of abiotic stresses either presence alone or in combination (s). However, there are two ways plants can survive against these abiotic stresses; a) through management practices and b) through adaptive mechanisms to tolerate plants. These adaptive mechanisms of tolerant plants are mostly linked to their signalling transduction pathway, triggering the action of plant …


Editorial: Genetic Validation And Its Role In Crop Improvement, Ahmed Sallam, Ahmad M. Alqudah, P. Stephen Baenziger, Awais Rasheed 2023 Assiut University, Leibniz Institute of Plant Genetics and Crop Plant Research

Editorial: Genetic Validation And Its Role In Crop Improvement, Ahmed Sallam, Ahmad M. Alqudah, P. Stephen Baenziger, Awais Rasheed

Agronomy & Horticulture -- Faculty Publications

Gene discovery for economically important traits has remained a challenging Frontier in crop genomics and breeding. The recent advances in DNA sequencing technologies and genetic analysis approaches paved the way for discovering many genes and hotspot genomic regions controlling target traits. The detection of novel genomic regions or candidate genes is very useful for plant breeders and geneticists to improve crops, dissect the genetics of complex traits, and understand the biological mechanisms of genes underpinning traits of interest. Quantitative trait loci (QTL) mapping and genome-wide association studies (GWAS) dominated recent crop gene discovery research. These studies are becoming routine activities …


Transformation And Gene Editing In The Bioenergy Grass Miscanthus, Anthony Trieu, Mohammad B. Belaff, Pradeepa Hirannaiah, Shilpa Manjunatha, Rebekah Wood, Yokshitha Bathula, Rebecca L. Billingsley, Anjali Arpan, Erik J. Sacks, Thomas E. Clemente, Stephen P. Moose, Nancy A. Reichert, Kankshita Swaminathan 2022 HudsonAlpha Institute for Biotechnology, University of Illinois Urbana-Champaign

Transformation And Gene Editing In The Bioenergy Grass Miscanthus, Anthony Trieu, Mohammad B. Belaff, Pradeepa Hirannaiah, Shilpa Manjunatha, Rebekah Wood, Yokshitha Bathula, Rebecca L. Billingsley, Anjali Arpan, Erik J. Sacks, Thomas E. Clemente, Stephen P. Moose, Nancy A. Reichert, Kankshita Swaminathan

Agronomy & Horticulture -- Faculty Publications

Background: Miscanthus, a C4 member of Poaceae, is a promising perennial crop for bioenergy, renewable bioproducts, and carbon sequestration. Species of interest include nothospecies M. x giganteus and its parental species M. sacchariforus and M. sinensis. Use of biotechnology-based procedures to genetically improve Miscanthus, to date, have only included plant transformation procedures for introduction of exogenous genes into the host genome at random, non-targeted sites.

Results: We developed gene editing procedures for Miscanthus using CRISPR/Cas9 that enabled the mutation of a specific (targeted) endogenous gene to knock out its function. Classified as paleo-allopolyploids (duplicated ancient …


Genetic Variation In Common Bunt Resistance In Synthetic Hexaploid Wheat, Amira M. I. Mourad, Alexey Morgounov, P. Stephen Baenziger, Samar M. Esmail 2022 Leibniz Institute of Plant Genetics and Crop Plant Research, Assiut University

Genetic Variation In Common Bunt Resistance In Synthetic Hexaploid Wheat, Amira M. I. Mourad, Alexey Morgounov, P. Stephen Baenziger, Samar M. Esmail

Agronomy & Horticulture -- Faculty Publications

Common bunt (caused by Tilletia caries and T. Foetida) is a major wheat disease. It occurs frequently in the USA and Turkey and damages grain yield and quality. Seed treatment with fungicides is an effective method to control this disease. However, using fungicides in organic and low-income fields is forbidden, and planting resistant cultivars are preferred. Due to the highly effective use of fungicides, little effort has been put into breeding resistant genotypes. In addition, the genetic diversity for this trait is low in modern wheat germplasm. Synthetic wheat genotypes were reported as an effective source to increase the …


Inheritance Of 2,4‑Dichlorophenoxyacetic Acid (2,4‑D) Resistance In Amaranthus Palmeri, Chandrima Shyam, Dallas E. Peterson, Amit J. Jhala, Mithila Jugulam 2022 Kansas State University

Inheritance Of 2,4‑Dichlorophenoxyacetic Acid (2,4‑D) Resistance In Amaranthus Palmeri, Chandrima Shyam, Dallas E. Peterson, Amit J. Jhala, Mithila Jugulam

Agronomy & Horticulture -- Faculty Publications

In this study, the inheritance of 2,4-D resistance in a multiple herbicide-resistant Palmer amaranth (KCTR) was investigated. Direct and reciprocal crosses were performed using 2,4-D-resistant KCTR and susceptible KSS plants to generate F1 progenies. 2,4-D dose–response assays were conducted to evaluate the response of progenies from each F1 family along with KCTR and KSS plants in controlled environmental growth chambers. Additionally, 2,4-D-resistant male and female plants from each of the F1 families were used in pairwise crosses to generate pseudo-F2 families. Segregation (resistance or susceptibility) of progenies from the F2 families in response to a …


Early Detection, Rapid Response Invasives Management: Documenting Perennial Pepperweed In New Hampshire Using Eddmaps, Final Project Report, December 2022, Malin Clyde 2022 University of New Hampshire, Durham

Early Detection, Rapid Response Invasives Management: Documenting Perennial Pepperweed In New Hampshire Using Eddmaps, Final Project Report, December 2022, Malin Clyde

UNH Cooperative Extension

No abstract provided.


A Stealth Health Approach To Dietary Fibre, P. Stephen Baenziger, Katherine Anna Frels, Steve Greenspan, Julie Jones, Alison Lovegrove, Devin J. Rose, Peter Shewry, Rod Wallace 2022 University of Nebraska at Lincoln

A Stealth Health Approach To Dietary Fibre, P. Stephen Baenziger, Katherine Anna Frels, Steve Greenspan, Julie Jones, Alison Lovegrove, Devin J. Rose, Peter Shewry, Rod Wallace

Agronomy & Horticulture -- Faculty Publications

Average dietary fibre intakes have increased little in the past twenty years in many countries, including the USA1 . Multi-million-dollar campaigns promoting fruits, vegetables, whole grains and other foods high in fibre have delivered only small changes in diets2 , and consumers have not changed from traditional staples to whole-grain options3 . UK millers report that consumption of whole-wheat bread has actually declined over the past decade (P. Shewry, personal communication). In the US, white flour, which is lower in fibre than whole-wheat flour, accounts for nearly 40% of the fibre intake4 . We believe that …


Genetic Variations Of Narcissus Tazetta And Selected Narcissus Cultivars Based On The Sequence Analysis Of Nrits And TrnL‑Is‑TrnF Regions, Seo Young Park, Mi Jin Jeon, Young Hee Joung, Keenan L. Amundsen, Gianluca Burchi, Angelo Porcelli, Mark S. Roh 2022 Chonnam National University

Genetic Variations Of Narcissus Tazetta And Selected Narcissus Cultivars Based On The Sequence Analysis Of Nrits And TrnL‑Is‑TrnF Regions, Seo Young Park, Mi Jin Jeon, Young Hee Joung, Keenan L. Amundsen, Gianluca Burchi, Angelo Porcelli, Mark S. Roh

Agronomy & Horticulture -- Faculty Publications

Narcissus tazetta var. chinensis (N. taz. var. chi.) accessions collected from the coastal areas of China, Korea, and Japan were compared with accessions of lower ranks of Narcissus tazetta (N. taz.) from Italy to study the genetic variations and with several morphologically similar cultivars available in the trade. Sequences of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrITS) dendrogram and the chloroplast trnL-IS-trnF intergeneric spacer-gene (cpIS) were analyzed by maximum likelihood (ML) and posterior probability using STRUCTURE program. All single fower form of N. taz. var. chi. collected from China, Korea, and …


Evaluation For Using Expended Bioelectrochemical Systems As Soil Amendments For Improved Corn Plant Growth And A Drought Resistant Soil., Jackson Lee Sauers 2022 Mississippi State University

Evaluation For Using Expended Bioelectrochemical Systems As Soil Amendments For Improved Corn Plant Growth And A Drought Resistant Soil., Jackson Lee Sauers

Theses and Dissertations

A long-held practice is to mix agricultural soil with a soil amendment to improve growing conditions in crops. A common soil amendment is biosolids produced from both municipal and dairy wastewater due to the macro- and micronutrients within it. Both the agricultural and wastewater industries are participating in the Circular Economy concept (CEC). Two experiments explored using expended bioelectrochemical systems (BES) that treated either synthetic dairy wastewater (DWW) or synthetic municipal wastewater (SWW) as soil amendments to improve corn plant growth when treated with three different nutrient treatments: 100%- 50%- and 0% Hoagland Nutrient Solutions. Biochar and used terracotta clay …


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