Plant Breeding and Genetics Commons^™

Arkansas Soybean Research Studies 2023, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

The 2023 Arkansas Soybean Research Studies includes research reports on topics pertaining to soybean across several disciplines from breeding to post-harvest processing. Research reports contained in this publication may represent preliminary or only data from a single year or limited results; therefore, these results should not be used as a basis for long-term recommendations.

Several research reports in this publication will appear in other University of Arkansas System Division of Agriculture’s Arkansas Agricultural Experiment Station publications. This duplication is the result of the overlap in research coverage between disciplines and our effort to inform Arkansas soybean producers of the research …

A Barley Pan-Transcriptome Reveals Layers Of Genotype-Dependent Transcriptional Complexity, Wenbin Guo, Miriam Schreiber, Vanda B. Marosi, Paolo Bagnaresi, Morten Egevang Jorgensen, Katarzyna B. Braune, Ken J. Chalmers, Brett Chapman, Viet Hoang Dang, Christoph Dockter, Anne Fiebig, Geoffrey B. Fincher, Agostino Fricano, John Fuller, Allison Haaning, Georg Haberer, Axel Himmelbach, Murukarthick Jayakodi, Yong Jia, Nadia Kamal, Peter Langridge, Chengdao Li

Grain and Other Field Crops Research Articles

A pan-transcriptome describes the transcriptional and post-transcriptional consequences of genome diversity from multiple individuals within a species. We developed a barley pan-transcriptome using 20 inbred genotypes representing domesticated barley diversity by generating and analyzing short- and long-read RNA-sequencing datasets from multiple tissues. To overcome single reference bias in transcript quantification, we constructed genotype-specific reference transcript datasets (RTDs) and integrated these into a linear pan-genome framework to create a pan-RTD, allowing transcript categorization as core, shell or cloud. Focusing on the core (expressed in all genotypes), we observed significant transcript abundance variation among tissues and between genotypes driven partly by RNA …

Arkansas Corn And Grain Sorghum Performance Tests 2024, J. F. Carlin, R. D. Bond, J. C. Mccoy, T. C. Swanson

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

The 2024 corn performance tests contained 44 hybrids and were conducted at the Northeast Rice Research and Extension Center (NERREC) at Harrisburg, the Lon Mann Cotton Research Station (LMCRS) near Marianna, the Rohwer Research Station (RRS) near Rohwer, and the Rice Research and Extension Center (RREC) near Stuttgart. The 2024 grain sorghum performance tests contained 16 …

Spatial Analysis Of American Chestnut (Castanea Dentata) Distribution And Ecology In Kentucky’S Federal Lands, Jacob Raymond Pease

Murray State Theses and Dissertations

Anthropogenic factors have been the cause of the decline of tree species across the globe. From unsustainable logging and development to disease and improper management, threats to forests have taken their toll. The American chestnut (Castanea dentata) was once an integral basal tree species in Appalachian forests, but chestnut blight drove the species to functional extinction by the mid-1900s. The recent creation of genetically modified chestnuts gives hope for future restoration of the species throughout its historical range. Despite blight resistance, effective restoration will require optimal site selection for finding and planting chestnuts, which will maximize limited resources. …

Identification Of A Stable Qtl For Juice Color In A Vitis Interspecific Hybrid ‘Chambourcin’- Based Population, Qiuni Yang

Graduate Theses/Dissertations

Berry juice color is one of the key factors influencing grape and wine quality, consumer preference, and marketability. While most previous studies on berry color have focused on the skin, research on juice color remains limited. This study investigated the genetic basis of berry juice color in a hybrid population derived from the Vitis interspecific hybrid ‘Chambourcin’ and V. vinifera ‘Cabernet Sauvignon’. A new nine-category visual scoring system was developed to assess juice color based on OIV 225. To achieve comprehensive precision and quantitative analysis, CIELAB parameters (L*, a*, b*), Hue, and Chroma were extracted from image-based measurements. Using berries …

2025 Standard-Sized Seedless Watermelon Cultivar Evaluation In Indiana, Wenjing Guan, Dennis Nowaskie, Cesar Escalante

Midwest Vegetable Trial Reports

Indiana ranked fifth in watermelon production in the United States. In 2024, Indiana growers planted approximately 8,100 acres of watermelons. Watermelon cultivar evaluation trials have been conducted annually at the Southwest Purdue Agricultural Center (SWPAC) in Vincennes, IN, for over 30 years. In 2025, 25 standard-sized seedless watermelons were evaluated, and yield and fruit quality parameters were assessed.

Optimization Of Transformation And Regeneration In The Sunflower (Helianthus Annuus), Sara M. Kilar

Masters Theses

In addition to their value as prized ornamentals, sunflowers are important crop species due to the nutritional value and high oil content of the seeds. Sunflower oil is a significant contributor to global seed oil production, which can be used as a food additive and cooking agent as well as a raw material for biodiesel production. While traditional breeding efforts have led to the development of many unique cultivars, genetically modified variations have proven more difficult due to the recalcitrance of sunflower plants to genetic transformation. As a result, facile and efficient transformation methods for sunflowers have the potential to …

Divergent Floral And Vegetative Trait Responses To Abiotic Factors Drive Phenotypic Diversity In Platanthera Dilatata (Orchidaceae) Across Alaska, Marlin L. Bowles, Lisa E. Wallace

Biological Sciences Faculty Publications

Multiple traits make up an integrated phenotype, but traits may be shaped by differing drivers. It is often assumed that selection by biotic agents drives floral variation, but abiotic factors are also known to influence floral traits. Understanding trait covariations and their association with diverse environmental factors is a central question in evolution and ecology. Here, we studied covariation in floral and vegetative traits in relation to climate and topo-edaphic variation in the orchid Platanthera dilatata. In Platanthera, nectar spur length is associated with pollinator tongue length, but much variation exists in other traits too, likely to be …

The Effect Of Thidiazuron Treatment On The Growth Of Vanda Limbata Blume In Vivo, Novika Ditasya Putri, Nintya Setiari, Yulita Nurchayati, Sri Widodo Agung Suedy

Makara Journal of Science

Vanda limbata is one of the most popular ornamental orchids. Currently, its growth is slow, making it necessary to improve its cultivation techniques. One of the possible ways to achieve this is to use plant growth regulator (PGR) treatment. Thidiazuron (TDZ) is a cytokinin used to increase the growth of V. limbata. This study aimed to determine the effect of TDZ concentration on the growth rate of V. limbata. The study involved cultivating V. limbata on planting media and spraying it with TDZ at various concentrations. This research uses a CRD (Completely Randomized Design) single-factor, which is TDZ concentration at …

Maize Hybrids’ Genetic Variability Based On Qualitative And Quantitative Traits, Slamet Bambang Priyanto, Herawati, Suwarti, Ayyub Ar Rahman, Nining Nurini Andayani, Abdul Fattah, Muhammad Azrai

Jurnal Kultivasi

Genetic variability was a prerequisite to doing a plant breeding program. A broad genetic variability allows plant breeders to select a desired genotype. This research aims to assess the maize hybrid's genetic variability based on qualitative and quantitative traits. This research was conducted in the Bone district, south Sulawesi, from November 2022 to March 2023. Fifteen maize hybrids were arranged in a randomized complete block design with three replications. The variables observed are qualitative and quantitative traits. Principal component (PCA) and cluster analyses assessed the genetic variability. The result indicated that based on a loading factor greater than 0.70, the …

Diversity Of Gibberellin 2-Oxidase Genes In The Barley Genome Offers Opportunities For Genetic Improvement, Jingye Cheng, Yong Jia, Camilla Hill, Tianhua He, Ke Wang, Ganggang Guo, Sergey Shabala, Meixue Zhou, Yong Han, Chengdao Li

Grain and Other Field Crops Research Articles

Introduction

Gibberellin (GA) is a vital phytohormone in regulating plant growth and development. During the “Green Revolution”, modification of GA-related genes created semi-dwarfing phenotype in cereal crops but adversely affected grain weight. Gibberellin 2-oxidases (GA2oxs) in barley act as key catabolic enzymes in deactivating GA, but their functions are still less known.

Objectives

This study investigates the physiological function of two HvGA2oxgenes in barley and identifies novel semi-dwarf alleles with minimum impacts on other agronomic traits.

Methods

Virus-induced gene silencing and CRISPR/Cas9 technology were used to manipulate gene expression of HvGA2ox9 and HvGA2ox8ain barley and RNA-seq …

Investigate The Possibility Of Producing Healthier, Safer Peanuts Through Molecular Breeding And Novel Fungal Pathogen Management Strategies, Tariq Alam

All Dissertations

Peanut allergy, a critical health concern with high morbidity and mortality rates, is a leading cause of fatal anaphylactic reactions in the United States. Managing peanut allergy is challenging due to the prevalence of peanuts in processed foods and the risk of contamination. This dissertation evaluates the U.S. peanut mini-core collection for four major immunogenic proteins, Ara h1, Ara h2, Ara h3, and Ara h6, using gel electrophoresis, enzyme-linked immunosorbent assay, liquid chromatography, and mass spectrometry. The study identified 26 genotypes with reduced concentrations of allergenic proteins. Genome-wide association studies (GWAS) of protein traits revealed 10 significant SNPs and identified …

Morphological And Physiological Changes Of Katuray (Sesbania Grandiflora) During Seed Maturation [Research Note], Alangelico San Pascual, Lucille Elna De Guzman, Maurice Gravidez, Elijah Estante

The Philippine Agricultural Scientist

Katuray (Sesbania grandiflora), whose flowers are consumed as steamed vegetables, is an important green manure crop in the Philippines. Understanding different processes leading to seed maturity can aid in determining S. grandiflora’s seed quality, preventing premature harvesting for seed increase, ensuring efficient propagation and production of seedlings, and breeding new varieties of this crop. Multiple flowers were tagged, and three to five pods were collected five days after anthesis (DAA) and at a five-day interval thereafter until 100% germination was achieved. Seed size, moisture content (MC), dry weight, germination percentage, and embryo development were observed and recorded. Seed length was …

Root Plasticity Of Selected Corn (Zea Mays L.) Varieties Grown For Food And Forage In Response To Fertigroe®-N And Urea, Vanica Apostol, Edna Aguilar, Lilia Fernando, Lourdes Edano Ma., Tonette Laude, Eureka Teresa Ocampo

The Philippine Agricultural Scientist

Root plasticity, the capacity of a corn root system to modify its growth and development in response to varying environmental conditions, is a crucial adaptability trait for optimizing water and nutrient uptake, especially in a challenging environments. Understanding root plasticity provides insights into the pattern basis of crop adaptation, contributing to the development of improved corn varieties and agricultural practices that optimize the production of both ear shoots and forage. This study assessed root traits and other morphological-physiological responses of three corn varieties (NK8840, IPB Var 6, and Pearl Sweet) grown for food (ear shoots) and forage (baby corn fodder) …

Comparison Of Statistical And Machine Learning Genomic Prediction Methods In Plant Breeding: Case Studies In Maize And Soybean, Igor Kuivjogi Fernandes

Graduate Theses and Dissertations

Plant breeding is essential to increase genetic gain and food production worldwide. This study was conducted to evaluate new ways to use machine learning (ML) to tackle plant breeding challenges, where two ideas were tested — the first chapter focuses on how to combine genetic and environmental data using ML to improve the prediction of maize grain yield in multi-environment trials, while the second chapter centers on how to couple feature selection of molecular markers with ML to enhance prediction of yield in soybean, and, in both cases, ML approaches were compared to well-established statistical methods greatly adopted by the …

Pangenome And Pantranscriptome As The New Reference For Gene-Family Characterization: A Case Study Of Basic Helix-Loop-Helix (Bhlh) Genes In Barley, Cen Tong, Yong Jia, Haifei Hu, Zhanghui Zeng, Brett Chapman, Chengdao Li

Grain and Other Field Crops Research Articles

Genome-wide identification and comparative gene-family analyses have commonly been performed to investigate species-specific evolution linked to various traits and molecular pathways. However, most previous studies have been limited to gene screening in a single reference genome, failing to account for the gene presence/absence variations (gPAVs) in a species. Here, we propose an innovative pangenome-based approach for gene-family analyses based on orthologous gene groups (OGGs). Using the basic helix-loop-helix (bHLH) transcription factor family in barley as an example, we identified 161–176 bHLHs in 20 barley genomes, which can be classified into 201 OGGs. These 201 OGGs were further classified into 140 …

Evaluation Of Cotton Advanced Lines/Varieties For Genetic Diversity And Correlation Studies Of Cotton Leaf Curl Virus Disease With Yield Contributing Traits In Cotton, Nouman Bilal, Wajid Nazeer, Abdul Latif Tipu, Shoaib Liaqat, Muhammad Sarwar, Ansar Ali, Khadim Hussain, Maria Basheer

Journal of Bioresource Management

Plant genetic diversity aids in the creation of new varieties that are more resistant to pests and unfavorable climatic conditions. In order to discover varied parents and assess their cross-performance, the current study was carried out. The findings showed a substantial positive connection (0.81) between green bolls, plant height (0.57), and open bolls, as well as a significant positive correlation (0.52) between open bolls. On the other hand, a highly significant negative connection was discovered between the number of open bolls (-0.59), number of green bolls (-0.72), plant height (-0.78), and major stem nodes (-0.70) for the cotton leaf curl …

Effects Of Soil Type, Irrigation Method, And Biomass Application On Grain Yield And Its Determinants In Irrigated Lowland Rice Of Agusan [Research Note], Jehru C. Magahud

The Philippine Agricultural Scientist

Information about the influence of soil type, irrigation method, and biomass use on rice productivity in Agusan is insufficient, despite their importance in developing or improving irrigated lowland rice production. Hence, this study determined the influence of these factors on grain yield and yield-contributing crop traits. It also explained grain yield based on yield determinants and recommended water management for improving grain yield. The greenhouse experiment was laid out in a three-factor randomized complete block design with 10 replications. The treatments were [a] Butuan sandy loam soil (SLS) and Butuan loam soil (LS); [b] continuously flooded (CF) and controlled irrigation …

Are You Planting Resistant Weeds With Your Crop Seed?, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Key messages

• West Australian (WA) grain growers have significant weed seed contamination in their crop seed.

• WA growers are sowing herbicide resistant weed seed into their cropping paddocks.

• Crop hygiene (harvest, seeding, transporting) is important to minimise spread of weeds.

Harvest is well underway throughout the WA grainbelt, and growers will be harvesting grain and collecting seed for next year’s crop.

However, this crop seed can be contaminated with weed seeds and, if seed cleaning operations are only partially effective, this weed seed may be sown into paddocks with crop seed in the following season.

The Effect Of Various Doses Of Gamma Ray Irradiation On Growth And Chlorophyll Changes Of Three Adlay Genotypes, Warid Ali Qosim, Anas, Suseno Amien, Meddy Rachmadi, Sidik Ramdani, Romi Zamhir Islami

Jurnal Kultivasi

Adlay has great potential to be developed into an alternative food source in Indonesia because it contains carbohydrates and high fat. The aim was to effect of various doses of gamma-ray irradiation on growth,chlorophyll changes, and determination of lethal dose (LD50) three adlay genotypes in M1 generation. This study was conducted by using the experimental quantitative method without design. The material used consists of three adlay genotypes, including genotypes #28, #37, and #38. The seeds were treated with 0 Gy (control), 100 Gy, 200 Gy, 300 Gy, 400 Gy, and 500 Gy doses of gamma-ray irradiation by the Research Center …