Physiology Commons^™

Effect Of Reduced Cardiomyocyte O-Glcnacylation On The Expression And Activity Of The Cardiac Voltage-Gated Sodium Channel, Chiagozie Dawn Bede Paul-Onyia

Browse all Theses and Dissertations

Abnormal glycosylation can impact cardiac proteins, affecting signaling, contraction, and metabolism, which leads to compromised cardiac function. O-GlcNAcylation, the only form of intracellular glycosylation, involves adding a single GlcNAc molecule to proteins via enzymes: O-GlcNAc Transferase (OGT) for addition and O-GlcNAcase (OGA) for removal. The Ednie/Bennett lab has previously demonstrated that in both chronic (OGT KO) and acute (OGT inhibition) models of reduced cardiomyocyte O-GlcNAcylation, a disruption of Ca2+ homeostasis occurs. This study delved into the cardiac voltage-gated sodium channel (NaV 1.5) in these models. NaV 1.5 expression was analyzed through western blotting and immunocytochemistry, while its functional activity was …

Regulation Of Motile Ciliogenesis And The Targeting Of Meig1/Pacrg/Dnali1 Complex For The Development Of Male Contraceptive, Yi Tian Yap

Wayne State University Dissertations

Cilia are broadly categorized into primary and motile cilia. The intraflagellar transport(IFT) system, composed of two large multimeric IFT complexes, is universally present in all cilia and facilitates bidirectional cargo transport. While the role of IFT in primary cilia and eukaryotic motile cilia has been demonstrated, the association between IFT and mammalian motile ciliogenesis remains largely unknown. Aim 1 of the present study is to uncover the role of IFT in mammalian motile ciliogenesis. By using a motile cilia-specific Ift140 knock-out mice, the present study demonstrates the significance of IFT140 in the formation and maintenance of cilia in the airways, …

Dimerization Of Dopamine (D2) And Adenosine (A2a) Receptors, Elena Vukovic, Jackson M. Macatol

Williams Honors College, Honors Research Projects

This experiment will aim to explore the heterodimerization of Dopamine (D2) and Adenosine (A2A) receptors in cells when bound to different ligands. Borroto-Escuela et al. (2018) investigated the dimerization of the A2AR-D2R in rats when administered with cocaine, and they found that disrupting the A2AR-D2R complex restores the cocaine reward system in rats. This was a strong influence as to why we chose to investigate A2AR-D2R dimerization in Chinese hamster ovary (CHO) cells.

One goal that we have for this experiment is to combine our current laboratory skills with new laboratory techniques. There are constantly new techniques in labs, and …

Effects Of Electronic Cigarettes On The Standard Metabolic Rate Of Crayfish, Frank Shaffer

Williams Honors College, Honors Research Projects

This study aims to examine the relationship between e-cigarettes (both with nicotine and without) and the effects on the standard metabolic rates of crayfish. In this experiment, 42 crayfish were divided into three treatment groups and exposed to a designated solution of either a control solution or a solution containing e-cigarette (nicotine or non-nicotine) vapor. Their standard metabolic rates were then measured by using oxygen uptake as a proxy. The results of this experiment indicate that there is no statistically significant relationship between the use of electronic cigarettes and the standard metabolic rate of crayfish. However, this study can be …

Repeated Occupational-Level Exposure To The Pesticide Malathion Leads To Neuronal Atrophy In The Dorsal Root Ganglion, Arian K. Mcneil

Browse all Theses and Dissertations

Environmental exposure to organophosphate (OP) pesticides, such as malathion, is a risk factor for neuropathy and neurodegeneration. Toxic levels of OPs irreversibly inhibit acetylcholinesterase (AchE) activity, leading to acute paralysis and even death as the neurotransmitter acetylcholine accumulates at cholinergic synapses. In addition, there is compelling evidence that repeated low-level “occupational-like” exposure to OPs is associated with somatosensory defects but the cellular mechanisms for this effect are unclear. We show sensory neuron cell size in the dorsal root ganglia is significantly reduced in rats exposed to occupational level malathion. However, co-administration of a reversible AchE inhibitor, galantamine, prevented this effect.

The Investigation Of Novel Bovine Oocyte-Specific Long Non-Coding Rnas And Their Roles In Oocyte Maturation And Early Embryonic Development, Jaelyn Zoe Current

Graduate Theses, Dissertations, and Problem Reports (ETD)

Early embryonic loss is a significant factor in livestock species' infertility, resulting in an economic deficit. In cattle, the in vivo fertilization rate is ~90%, with an average calving rate of about 55%, indicating an embryonic-fetal mortality rate of roughly 35%. Further, 70-80% of total embryonic loss in cattle occurs during the first three weeks after insemination, particularly between days 7-16. Growing evidence indicates that the oocyte plays an active role in regulating critical aspects of the reproductive process required for successful fertilization, embryo development, and pregnancy. However, defining oocyte quality remains enigmatic. Recently, many have abandoned the notion that …

Sex Differences In Endothelial Function, Metabolism, And Sleep: Responses To Disrupted Circadian Rhythms, Olga Hecmarie Meléndez-Fernández

Graduate Theses, Dissertations, and Problem Reports (ETD)

Circadian rhythms are endogenous cycles that recur approximately every 24 h, and manifest in virtually every physiological and behavioral process among the vast majority of organisms. These rhythms, sustained by the central circadian clock, the suprachiasmatic nucleus (SCN) of the hypothalamus, allow for optimal timing of biological processes that ensure organisms’ homeostasis, and are entrained to precisely 24 h by daily exposure to light. Disruption of the molecular mechanisms that drive these rhythms have negative consequences on physiology and behavior that may impair survival. The molecular mechanisms that underlie these effects are widely studied and linked to multiple diseases and …

Multilevel Phenotypic Integration Of Metabolism And Behavior In House Sparrows And Mice, Tim Salzman

Theses and Dissertations--Biology

Patterns of multilevel variation in behavior, both within and among individuals, raise challenging questions about underlying mechanisms and the selective pressures acting on them. One intriguing hypothesis is that physiology shows parallel multilevel variation, and so might represent a latent trait that integrates multilevel behavioral responses. For example, foraging acquires the fuel needed to maintain metabolism, and in turn, an individual’s metabolism affects expression of foraging, and other, behaviors. Metabolism and behavior thus might coevolve to become integrated traits. Despite the appeal of this hypothesis, numerous investigations into the link between metabolism and behavior have yielded largely equivocal results.

To …

Bilirubin Levels Are Negatively Correlated With Adiposity In Obese Men And Women, And Its Catabolized Product, Urobilin, Is Positively Associated With Insulin Resistance, Zachary A. Kipp, Mei Xu, Evelyn A. Bates, Wang-Hsin Lee, Philip A. Kern, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

Bilirubin levels in obese humans and rodents have been shown to be lower than in their lean counterparts. Some studies have proposed that the glucuronyl UGT1A1 enzyme that clears bilirubin from the blood increases in the liver with obesity. UGT1A1 clearance of bilirubin allows more conjugated bilirubin to enter the intestine, where it is catabolized into urobilin, which can be then absorbed via the hepatic portal vein. We hypothesized that when bilirubin levels are decreased, the urobilin increases in the plasma of obese humans, as compared to lean humans. To test this, we measured plasma levels of bilirubin and urobilin, …

Use Of Hematological Markers To Assess Physiological Condition And Health Status In Free-Ranging Sand Tiger Sharks (Carcharias Taurus), Chestina N. Craig

College of Graduate Studies: Theses & Dissertations

The contents of blood can provide information about the physiological condition and health of vertebrates. This study seeks to better understand the stress physiology and blood bacteria presence of the sand tiger shark (Carcharias taurus), as sharks are known to have unique physiology and immune systems. In this study the blood metabolites glucose, lactate, and ketones (3-hydroxybuteric acid and acetoacetate), were used to understand how biotic and abiotic factors affect the acute stress response to capture and handling. Metabolite concentrations from blood plasma were analyzed using colorimetric assays. Glucose and ketones showed no significant responses to capture and …

The Association Of Vitamin D, Bone Mineral Density, And Injuries Among Acrobatics And Tumbling Athletes, K Lee, A Gallucci, J Forsse, L Cherpe De Souza, L Irvin, E Boyer, L Funderburk

Clinical Practice in Athletic Training

Introduction: Acrobatics and Tumbling (A&T) is an emerging sport incorporating various elements of gymnastics and competitive cheerleading. Anecdotal evidence suggests that the sport presents with high injury rates potentially due to the high impact nature of the activity. Due to the potential aesthetic sport related concerns, bone mineral density and risk of injury presents a concern for patients, athletic trainers and stakeholders. Increased understanding of the relationship between bone mineral density, serum vitamin D status, and injury incidence in A&T may be useful to athletic trainers, registered dietitians, and others working with these athletes. The objective of this study was …

A Case Study On Treatment Of Subcoracoid Impingement In An Overhead Athlete With Manual Therapy, M Mennell

Clinical Practice in Athletic Training

Introduction: Subcoracoid impingement of the shoulder is a condition that occurs when the tendons or soft tissues in the shoulder are compressed or pinched between the coracoid process and the humeral head. It is also suspected that subcoracoid impingement can occur when the humerus translates anteriorly, decreasing the subcoracoid space. This impingement can cause pain, inflammation, and limited mobility in the shoulder joint. All kinds of impingement are typically multifactorial, and have several areas that need to be addressed. The purpose of this case report is to examine the effects of therapeutic exercise as well as manual therapy on subcoracoid …

Childhood Mental Health Outcomes Following Mild Traumatic Brain Injury: An Evidence-To-Practice Review Leona J. Keller, Ms, Atc*; Sooji M. Berthiaume, Ms, Atc†; Karis J. L, Leona J. Keller, Sooji M. Berthiaume, Karis J. Landry, Alyssa L. Bolno, Zachary K. Winkelmann

Clinical Practice in Athletic Training

Concussions can have lasting symptoms in children such as depression, anxiety, hyperactivity, or failure to control anger. If diminished mental health outcomes are not monitored and do not improve after concussion, children may need additional treatment as a result of sequela. The purpose of the guiding systematic review was to determine if there are increased mental health symptoms in pediatric patients who sustained a concussion as compared to pediatric patients who have not sustained a concussion. The guiding systematic review and meta-analysis authors used seven different databases for articles published from 1980 to 2020. Selected articles had mental health outcomes …

Attitudes Toward Implicit Bias Among Ncaa Medical Support Staff, B J. Warner, A P. Jacobsen, M Decker, S A. Cage

Clinical Practice in Athletic Training

Introduction: Implicit biases are unconscious attitudes, emotions, or stereotypes that have the potential to negatively affect behaviors, actions, and decisions. Recent research has shown that healthcare workers do not provide equitable care to patients from different demographics. When patients are receiving different levels of care, there is a potential for different patient-related outcomes. One of their first steps in many implicit bias interventions for healthcare professionals is identifying one’s implicit bias. The purpose of this study was to describe the attitudes toward implicit bias among non-athletic training healthcare professionals who provide care to collegiate student-athletes. Methods: An electronic survey was …

Intrinsic Foot Muscle Training For Medial Tibial Stress Syndrome, Katherine Newsham, Michelle Boyd, Christine Lauber

Clinical Practice in Athletic Training

Medial tibial stress syndrome (MTSS), a common condition in physically active individuals, is widely studied, yet effective and efficient intervention is elusive. We compared the effects of a 2-week neuromuscular-based intervention to the “usual treatment” in a non-randomized trial involving intercollegiate athletes with complaints of MTSS involving soft tissue. The neuromuscular-based intervention focused on relieving trigger points in the deep compartment and improving intrinsic foot muscle (IFM) function. The control group received “usual treatment” that did not include the study exercises, but did include rest, modalities, stretching, and/or strengthening exercises. Pre- and post-intervention measures included pain at rest, during activities …

Interventions For The Mind, Body, And Spirit Of Ncaa Student-Athletes: An Evidence To Practice Review, Molly M. Osborn, Victoria A. Williams, Allison B. Smith, Zachary K. Winkelmann

Clinical Practice in Athletic Training

Life as an athlete in the National Collegiate Athletic Association (NCAA) can enrich and play an integral part in a young student-athlete's development. Consequently, this lifestyle comes with its own stressors that affect one’s physical, spiritual, and psychosocial well-being. These issues can affect various aspects of a student-athlete’s life, including their performance in sports, school, and social settings. Recent studies have begun connecting these domains directly to the health and well-being of NCAA student-athletes, leading to new evidence focused on the best intervention protocols for the biopsychosocial-spiritual (BPSS) model of health. A systematic review was conducted using five electronic databases. …

Providing Athletic Training Resources To Deaf And Hard-Of-Hearing Persons, Molly M. Osborn, Courtney M. Meyer, Zachary K. Winkelmann

Clinical Practice in Athletic Training

No abstract provided.

Does Pressure From Coaches Affect The Eating Habits Of Male Collegiate Athletes?, C Bynum, M Long

Clinical Practice in Athletic Training

Introduction: Research has shown that eating disorders tend to be most seen in females and athletes. The research on the prevalence of eating disorders in males is low compared to the research on females. This study examined male athletes at Abilene Christian University and determine if the pressure about weight from coaches and teammates could influence the eating habits of these athletes. The purpose of this study is to determine if pressure about weight from coaches or teammates affected eating habits of male collegiate athletes at a NCAA Division 1 institution. Methods: This study employed a cross-sectional survey design. Participants …

Slowing It Down: The Impact Of A Core Stabilization Program Of Hypermobile Sprinters, T Spears, J Gonzalez-Pons, J Debose

Clinical Practice in Athletic Training

Background: Low back pain (LBP) is a common complaint in sprinters, and it can present itself in many ways. One study published in 2019 surveyed 2539 runners; 22.6% of them reported LBP of some kind at least once within that year.5 LBP can occur in sprinters for many reasons. One study noted that muscle weakness, imbalance, and improper recruitment of hip and/or core musculature are a few sources of LBP in athletes.4 The aim of this study is to examine the effectiveness of a core stabilization program on athletes suffering from lumbar facet hypermobility. Differential Diagnosis: Conditions that are typically …

Low Ferritin Count In Female Collegiate Soccer Player Presents As Concussion, E N. Nelson, T D. Pitcock, M K. Knoblauch, L J. Harrison

Clinical Practice in Athletic Training

Background: Women’s soccer leads the National Collegiate Athletic Association for having the highest number of concussions in female sports. Common symptoms of concussion can include light headedness, headaches, and dizziness, but these symptoms are not exclusive to concussions. Female athletes may experience nutritional deficits that can lead to a wide range of symptoms mimicking those commonly experienced with a concussion such as headaches, dizziness, and fatigue. The patient in this case is a 21- year-old female soccer athlete who presented with sensitivity to light, light headedness, nausea, and fatigue after experiencing repeated ‘headers’ as well as a collision during a …