Role Of Ampk In The Upregulation Of Steroidogenic Acute Regulatory Protein In The Zona Fasciculata Of The Adrenal Cortex,
2010
Brigham Young University - Provo
Role Of Ampk In The Upregulation Of Steroidogenic Acute Regulatory Protein In The Zona Fasciculata Of The Adrenal Cortex, Adam Wesley Dayton
Theses and Dissertations
Cortisol is a glucocorticoid produced by the zona fasciculata (ZF) of the adrenal cortex. Traditionally, cortisol production and release was seen as being regulated strictly by adrenocorticotropic hormone (ACTH). While this is true of baseline cortisol levels and in response to acute mental stress, the picture is somewhat more complicated in other situations.Interleukin-6 (IL-6) contributes to the maintenance of cortisol levels in situations of prolonged immune or inflammatory stress. AMP activated protein kinase (AMPK) was investigated as a possible mediator of the action of IL-6 or as an independent actor in raising cortisol levels in response to hypoxemic or hypoglycemic …
In Vivo Measurement Of Rat Skeletal Muscle Oxygen Consumption Following Brief Periods Of Ischemia With Reperfusion As Assessed By Phosphorescence Quenching Microscopy,
2010
Virginia Commonwealth University
In Vivo Measurement Of Rat Skeletal Muscle Oxygen Consumption Following Brief Periods Of Ischemia With Reperfusion As Assessed By Phosphorescence Quenching Microscopy, William Nugent
Theses and Dissertations
Brief periods of skeletal muscle ischemia (ischemic pre-conditioning) alter cellular metabolism in a way that confers protection over subsequent ischemic episodes. The mechanisms behind this effect have been studied indirectly through assays for the byproducts of ATP synthesis and in vitro studies of cellular signaling cascades and ROS generation. There have been no direct, in vivo assessments of the changes in respiration during reperfusion. We employed phosphorescence quenching microscopy in conjunction with a flow-arrest technique to assess the influences of external, pressure-induced 1- to 10-min focal ischemia on interstitial oxygenation (PISFO2) and the consumption of oxygen (VO2) in spinotrapezius muscles …
Kinematic Evidence For Superfast Locomotory Muscle In Two Species Of Teneriffiid Mites,
2010
Pomona College
Kinematic Evidence For Superfast Locomotory Muscle In Two Species Of Teneriffiid Mites, Grace C. Wu, Jonathan C. Wright, Dwight L. Whitaker, Anna N. Ahn
All HMC Faculty Publications and Research
Locomotory muscles typically operate over a narrow range of contraction frequencies, characterized by the predominant fiber types and functional roles. The highest documented frequencies in the synchronous sound-producing muscles of insects (550 Hz) and toadfish (200 Hz) far exceed the contraction frequencies observed in weight-bearing locomotory muscles, which have maximum documented frequencies below 15-30 Hz. Laws of scaling, however, predict that smaller arthropods may employ stride frequencies exceeding this range. In this study we measured running speed and stride frequency in two undescribed species of teneriffiid mites from the coastal sage scrub of southern California. Relative speeds of both species …
Discovery Of A Novel Ccr5 Antagonist As An Effective Therapeutic Agent For Prostate Cancer,
2010
Virginia Commonwealth University
Discovery Of A Novel Ccr5 Antagonist As An Effective Therapeutic Agent For Prostate Cancer, Tasrif Ahmed
Theses and Dissertations
Previously, the CCR5 receptor was found to be a good target for treating prostate cancer (PCa). Dr. Yan Zhang’s laboratory designed several CCR5 antagonists, which were screened for their inhibitory effect on the growth and invasion of the M12, DU145 and PC-3 PCa cell lines. Primary in vitro screening showed one compound (Drug 17) significantly inhibited the proliferation of PCa cells at 1μM concentration, with a half-maximal inhibitory concentration of 237.68 nM. Further in vitro assays including a proliferation, cytotoxicity and invasion assay confirmed the inhibitory effect of drug 17. The physiological effect of drug 17 was tested by the …
Poxa, Yjek And Elongation Factor P Coordinately Modulate Virulence And Drug Resistance In Salmonella Enterica,
2010
University of Toronto
Poxa, Yjek And Elongation Factor P Coordinately Modulate Virulence And Drug Resistance In Salmonella Enterica, William Wiley Navarre, Shicong Zou, Hervé Roy, Jinglin Lucy Xie, Alexei Savchenko, Alexander Singer, Elena Edvokimova, Lynne R. Prost, Runjun Kumar, Michael Ibba, Ferric C. Fang
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-β-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, including attenuated virulence in mice, an increased ability to respire under nutrient-limiting conditions, hypersusceptibility to a variety of diverse growth inhibitors, and altered expression of multiple proteins, including several encoded on the SPI-1 pathogenicity island. PoxA mediates posttranslational modification of bacterial elongation factor P (EF-P), analogous to the modification of the eukaryotic EF-P homolog, eIF5A, with …
Characterization And Crystallization Of The Mycobacterium Tuberculosis Trmd,
2010
Virginia Commonwealth University
Characterization And Crystallization Of The Mycobacterium Tuberculosis Trmd, Zohal Hamidi
Theses and Dissertations
One third of the world’s population is affected by Tuberculosis (TB), a disease caused by infection with Mycobacterium tuberculosis (MtB). The emergence of multidrug-resistant MtB makes this disease a major public health concern. New agents are needed to treat TB infections in a manner that circumvents existing pathways of resistance. One strategy is to target the organism at the translational level by inhibiting vital modifications of RNA. One gene responsible for these modifications is the tRNA (guanosine-1)-methyltransferase, trmD, which has been shown to be essential in several bacteria. The eukaryotic and bacterial m1G methyltransferases are structurally dissimilar, making this enzyme …
Distorting The Sarcomere,
2010
University of Kentucky
Distorting The Sarcomere, Kenneth S. Campbell
Physiology Faculty Publications
No abstract provided.
The Effect Of Cobalt Protoporphyrin And Cobalt Chloride On Heme Oxygenase Expression And Protection From Deoxycholate-Induced Apoptosis,
2010
Virginia Commonwealth University
The Effect Of Cobalt Protoporphyrin And Cobalt Chloride On Heme Oxygenase Expression And Protection From Deoxycholate-Induced Apoptosis, Tina Lawson
Theses and Dissertations
The inner surface of the stomach is lined by a mucous membrane known as the gastric mucosa. The integrity of the gastric mucosa is critical for protecting the stomach from the low pH and proteolytic environment within the lumen. Both clinically and experimentally, exposure of gastric mucosal cells to bile salts is known to cause injury. Bile salts present in duodenogastric reflux are thought to play a significant role in gastric ulcer formation and alkaline gastritis. In vitro, studies using physiologic concentrations of the secondary bile salt, deoxycholic acid, indicate that bile salts can induce apoptosis in cultured human gastric …
The Impact Of A Pilot Community Intervention On Health-Related Fitness Measures In Overweight Children,
2010
Middle Tennesee State University
The Impact Of A Pilot Community Intervention On Health-Related Fitness Measures In Overweight Children, Jenny G. Hutchens, Jennifer L. Caputo, Janet M. Colson, Richard S. Farley, Matthew S. Renfrow, Eric P. Seguin
International Journal of Exercise Science
The purpose of this study was to pilot a 5-week community-based intervention on improving measures of health-related fitness in overweight children. Data were obtained from 8 overweight and obese 8- to 14-year-old children. Measurements included muscular fitness (curl-ups and modified pull-ups), aerobic capacity (20 meter progressive aerobic cardiovascular endurance run [PACER] test), body composition (tricep and calf skinfolds), body mass index (BMI), and flexibility (back saver sit-and-reach). A significant reduction in BMI was observed at post-test compared to baseline (p = .03). There was a significant decrease in body fat at post-test for boys (p = .013).
Resistance And Aerobic Training Sequence Effects On Energy Consumption In Females,
2010
University of Central Missouri
Resistance And Aerobic Training Sequence Effects On Energy Consumption In Females, Rachael Cutts, Steve Burns
International Journal of Exercise Science
Purpose: The objective of this study was to investigate the effect of sequence of resistance and aerobic training on energy consumption on sedentary overweight females. Methods: Participants were 15 sedentary overweight females (age = 28.6 ±12 yrs; BMI = 28.1±7.8) Subjects did a counterbalanced intervention: resistance training (circuit training) first (intervention RT) or aerobic exercise first (intervention AT), while oxygen consumption was continuously measured for 80 min. Subjects performed a warm-up on the treadmill at 40% of their heart rate reserve for 5 minutes, then for 30 minutes did continuous walking or jogging on the treadmill at ~67% of their …
Longitudinal, Diet-Induced Weight Gain Is Associated With Increased Blood Monocytes And Reduced Tlr4 Expression,
2010
University of Houston - Main
Longitudinal, Diet-Induced Weight Gain Is Associated With Increased Blood Monocytes And Reduced Tlr4 Expression, Kelley Strohacker, Richard J. Simpson, Katie C. Carpenter, Whitney L. Breslin, Brian K. Mcfarlin
International Journal of Exercise Science
Excessive weight gain increases systemic inflammation resulting in increased disease risk. Toll-like receptor 4 (TLR4) reportedly mediates increases in inflammation; however, its role has not been fully evaluated. Objective. The purpose of this study was to determine the longitudinal effect of diet-induced weight gain on blood monocyte concentration and cell-surface TLR4 expression. Research Methods & Procedures. Male CD-1 mice were randomly assigned to high-fat (HF, n = 12) or low-fat (LF, n = 13) groups. Non-lethal, saphenous vein blood samples were collected at 0, 4, 8 and 12 weeks of treatment. Three-color flow cytometry was used to measure monocyte (CD11b+/CD14+) …
Does A Physical Education Bout Alter Percent Body Fat And The Adiposity Health Risk Classification When Using Leg-To-Leg Bioelectrical Impedance Analysis In Girls?,
2010
Bloomsburg University
Does A Physical Education Bout Alter Percent Body Fat And The Adiposity Health Risk Classification When Using Leg-To-Leg Bioelectrical Impedance Analysis In Girls?, Elise Fitzgerald, Angela Fatool, Curt B. Dixon, Joseph L. Andreacci
International Journal of Exercise Science
Purpose: The purposes of this investigation were to determine: 1) whether a structured in-school physical education exercise bout altered percent body fat (%BF) determined by leg-to-leg bioelectrical impedance analysis (LBIA); and 2) whether the potential exercise-induced %BF magnitude of change altered the health risk classification of the child. Methods: Seventy-six girls (age: 12.2 ± 1.0 yr; height: 153.9 ± 7.5 cm; body mass: 51.9 ± 15.5 kg; BMI: 21.7 ± 5.4 kg/m2) participated in this investigation. LBIA measured %BF values were obtained immediately before and within five minutes after completing a structured, in-school, physical education class. Results: Significant reductions (p < 0.0001) in mean %BF (25.0 ± 10.2 vs. 24.4 ± 10.3 %) were observed for the group following the physical education class. For the majority of the girls (88%), the %BF alteration was less than ± 2.0 %BF. More specifically, the %BF magnitude of change was ± 1.0 %BF in 64.5% of the girls, between 1.1 and 2.0 %BF in 23.7% of the girls, and by more than 2.0 %BF in 11.8% of the girls. Regardless of the %BF magnitude of change, all girls remained in the same adiposity classification category (healthy vs. unhealthy body fat) following exercise. Conclusion: Adhering to the pre-test exercise guideline appears unnecessary when using LBIA to categorize the health risk of an adolescent girl.
Does Standing On A Cycle-Ergometer, Towards The Conclusion Of A Graded Exercise Test, Yield Cardiorespiratory Values Equivalent To Treadmill Testing?,
2010
Southern Arkansas University-Magnolia
Does Standing On A Cycle-Ergometer, Towards The Conclusion Of A Graded Exercise Test, Yield Cardiorespiratory Values Equivalent To Treadmill Testing?, Joshua Mitchell, William B. Kist, Kendall Mears, Jesse Nalls, Kyle Ritter
International Journal of Exercise Science
Graded exercise testing (GXT), per a cycle-ergometer (CE), offers safety and monitoring advantages over treadmill (TM) GXT. Unfortunately, CE-VO2max and some other cardiorespiratory (CR) variables are frequently lower than TM-GXT values. It has been difficult to compare TM and CE-GXT values. However, it was hypothesized that standing towards the conclusion of the CE-GXT (Stand-CE) might increase CE values to those equal to TM-GXT. If Stand-CE and TM-GXT CR values were equal, Stand-CE-GXT could become the method of choice for GXT for the general population. The purpose of this investigation was to investigate the effect of Stand-CE on CR variables. An …
Perceived Muscle Soreness In Recreational Female Distance Runners,
2010
University of Central Missouri
Perceived Muscle Soreness In Recreational Female Distance Runners, Dave Burnett, Steve Burns, Kevin Smith
International Journal of Exercise Science
ABSTRACT PERCEIVED MUSCLE SORENESS IN FEMALE RUNNERS D. Burnett†, K. Smith†, S. Burns‡, C. Smeltzer*, K. Young†. Health and Human Performance. University of Central Missouri, Warrensburg, Mo; email: [email protected] Purpose: The purpose of this study was to determine if rating of perceived exertion correlated with perceived muscle soreness during delayed onset muscle soreness (DOMS) in female runners. This study examined the pre and post running economy measures and perceived muscle soreness before and after a 30 minute downhill run (DHR) at - 15% grade and 70% of the subjects predetermined maximum oxygen uptake (VO2 peak). Methods: Six female recreational runners …
Monitoring Training Load In Indian Male Swimmers.,
2010
Sports Authority of India,Bangalore.
Monitoring Training Load In Indian Male Swimmers., Pralay Majumdar Dr., Sri Vidhya Jr.
International Journal of Exercise Science
The present study was taken up to monitor the training load and the impact of training load to the hormone concentrations such as testosterone, cortisol and T/C (Testosterone/Cortisol) ratio during the three phases of training viz preparatory phase, pre-competitive phase and competitive phase in Indian male swimmers. Blood samples were collected at the completion of each phase and the hormone concentrations were quantified. Our results reveal that the testosterone and T/C ratio significantly decreases whereas cortisol increases in the subsequent periodised cycle and concludes that the hormone concentration changes with the intensity and duration of exercise i.e. when the intensity …
Association Of Body Surface Area And Body Composition With Heart Structural Characteristics Of Female Swimmers,
2010
IA University, Najafabad Branch, Isfahan
Association Of Body Surface Area And Body Composition With Heart Structural Characteristics Of Female Swimmers, Bahreh Sheikh Saraf, Hojat Allah Nikbakht, Mohammad Ali Azarbayjani
International Journal of Exercise Science
In healthy nonathletic populations, some left ventricle (LV) parameters such as LV mass (LVM) and LV end diastolic dimension (LVEDD) can be predicted by some of body size parameters such as body surface area (BSA), fat-free mass (FFM), and height (H). These body size parameters use to remove covariate influence of body size from cardiac dimension variables and allow comparisons to be made between individuals and groups of different body size. Endurance exercise has been associated with changes in LV size and body composition of athletes. The aim of this study was to evaluate in 30 trained female swimmers (age …
Objective And Subjective Assessments Of ‘Normal’ Walking Pace, In Comparison With That Recommended For Moderate Intensity Physical Activity,
2010
Teesside University, Middlesbrough, UK
Objective And Subjective Assessments Of ‘Normal’ Walking Pace, In Comparison With That Recommended For Moderate Intensity Physical Activity, Kathryn L. Taylor, Claire Fitzsimons, Nanette Mutrie
International Journal of Exercise Science
Abstract Despite its common application and widely reported health benefits, walking, in relation to pace and intensity, is under-researched. Few studies have addressed whether people normally walk at a pace which meets the public health recommendations for moderate intensity physical activity (1.34-1.79 ms-1) and there is no known research on individuals’ perceptions of factors which influence walking pace. This study aimed to objectively assess if participants were reaching the pace required for moderate intensity physical activity during ‘normal’ walking. This was examined via a Global Positioning System (GPS) over a 1 km outdoor walk and a timed 150 m trial. …
Prevalence Of Plagiarism In Manuscript Submissions And Solutions,
2010
University of Houston - Main
Prevalence Of Plagiarism In Manuscript Submissions And Solutions, Brian K. Mcfarlin, T Scott Lyons, James W. Navalta
International Journal of Exercise Science
No abstract provided.
Recreational Cyclists: The Relationship Between Low Back Pain And Training Characteristics,
2010
James Cook University
Recreational Cyclists: The Relationship Between Low Back Pain And Training Characteristics, Samantha J. Schulz, Susan J. Gordon
International Journal of Exercise Science
This study investigated the relationship between low back pain (LBP) and training characteristics in recreational cyclists. Purposive sampling was used to recruit sixty-six recreational cyclists from nine cycling clubs. Participants completed a survey reporting training characteristics and LBP behaviour during a usual week of cycling. This included percent of time spent cycling in three common riding positions, cycling terrain, average cycling pace, number of gears, days per week cycled and number of cycling events per year. Fifty percent reported LBP during or after cycling or smoking and LBP. Cyclists who reported LBP cycled significantly further in a usual week of …
Delayed Wound Healing: Can Exercise Accelerate It?,
2010
Bowling Green State University
Delayed Wound Healing: Can Exercise Accelerate It?, K Todd Keylock, Hilary Young
International Journal of Exercise Science
Poorly healing wounds affect millions around the world, yet preventive methods and low-cost, effective treatments are few. Wounds heal quickly through well-coordinated phases in those who are healthy and active but can become chronically nonhealing as a result of disease and inactivity. Recently it has been reported that moderate aerobic exercise accelerated healing rates in the aged. High levels of inflammation are known to delay wound healing, and aging and disease are associated with chronically increased inflammation. Therefore, exercise may help speed healing by reducing inflammation to healthier levels not only in the aged, but also in other populations with …
