Physiology Commons^™

An Archaeal Trna-Synthetase Complex That Enhances Aminoacylation Under Extreme Conditions, Vlatka Godinic-Mikulcic, Jelena Jaric, Corinne D. Hausmann, Michael Ibba, Ivana Weygand-Durasevic

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Aminoacyl-tRNA synthetases (aaRSs) play an integral role in protein synthesis, functioning to attach the correct amino acid with its cognate tRNA molecule. AaRSs are known to associate into higher-order multi-aminoacyl-tRNA synthetase complexes (MSC) involved in archaeal and eukaryotic translation, although the precise biological role remains largely unknown. To gain further insights into archaeal MSCs, possible protein-protein interactions with the atypical Methanothermobacter thermautotrophicus seryl-tRNA synthetase (MtSerRS) were investigated. Yeast two-hybrid analysis revealed arginyl-tRNA synthetase (MtArgRS) as an interacting partner of MtSerRS. Surface plasmon resonance confirmed stable complex formation, with a dissociation constant (KD) of 250 nm. Formation of the MtSerRS·MtArgRS complex …

Proteoliposome Proton Flux Assays Establish Net Conductance, Ph-Sensitivity, And Functional Integrity Of A Novel Truncate Of The M2 Ion "Channel" Of Influenza A, Emily Peterson

Theses and Dissertations

A novel truncate of Influenza A M2 protein (residues 22-62), incorporated into a uniquely tailored proteoliposome proton uptake assay, demonstrated proton flux more characteristic of an ion transporter than a traditional ion "channel." The liposome paradigm was essential for testing the conductance activity of this M2 truncate at a range of extraphysiological pHs appropriate for channel vs. transport function determination. In addition to transporter-typical proton flux, M2(22-62) showed the key characteristics of functional integrity: selective proton uptake into liposomes and block of uptake by amantadine. Two sets of proteoliposome proton flux assays were carried out, Set 1 at pH values …

Characterization Of The Lkb1-Mo25-Strad Ampkk Complex In Adult Mouse Skeletal Muscle, Cody Don Smith

Theses and Dissertations

In liver tissue, the AMP-activated protein kinase kinase (AMPKK) complex was identified as the association of LKB1, MO25α/β, and STRADα/β proteins; however, this complex has yet to be characterized in skeletal muscle. In this report, we demonstrate the expression of the LKB1-MO25-STRAD AMPKK complex in adult skeletal muscle, confirm the absence of mRNA splice variants, and report the relative mRNA expression levels of these complex-forming proteins. To facilitate this characterization we used control (ctrl) and muscle-specific LKB1 knockout (LKB1-/-) mice. LKB1 detection in untreated ctrl and LKB1-/- muscle lysates revealed two protein bands at approximately 50 and 60 kDa; although, …

Evaluation Of Metabolic Stress Between Jumping At Different Cadences On The Digi-Jump Machine, Thomas S. Lyons, James W. Navalta, Zachary J. Callahan

International Journal of Exercise Science

The American College of Sports Medicine (ACSM) recommends that healthy adults achieve a minimum of thirty minutes of moderate intensity aerobic exercise five days per week. While cycling, walking, and jogging are commonly observed methods of achieving these recommendations, another option may be repetitive jumping. The purpose of this study was to examine the metabolic responses between repetitive jumping at a cadence of 120 jumps per minute (JPMs) vs. 100 JPMs when utilizing the Digi-Jump machine. Twenty-eight subjects completed two jumping trials, one at 120 JPMs and one at 100 JPMs. Subjects jumped until volitional exhaustion, or for a maximum …

Aerobic And Anaerobic Changes In Collegiate Male Runners Across A Cross-Country Season, Cory W. Baumann, Thomas J. Wetter

International Journal of Exercise Science

The purpose of this study was to assess the physiological characteristics of trained NCAA Division III male runners across a competitive season of cross-country. Eight male distance runners (age 20.6±1.4 y) were administered a battery of aerobic and anaerobic laboratory tests at the beginning and end of an 8-10 week racing season. Aerobic testing included maximal oxygen uptake (VO2max), running economy (RE), ventilatory threshold (VT) and the onset of blood lactate accumulation (OBLA). Anaerobic testing consisted of the vertical jump (VJ) and the Wingate test. Final testing revealed anaerobic Wingate peak power significantly declined (11.8±1.1 to 10.7±1.0 W·kg-1) (P = …

Effect Of Single Set Dynamic And Static Stretching Exercise On Jump Height In College Age Recreational Athletes, Jeffrey C. Murphy Dr., Elizabeth F. Nagle, Robert J. Robertson Dr., Jean L. Mccrory Dr.

International Journal of Exercise Science

This study examined the effects of single set dynamic and static stretching on vertical jump height and hip and knee range of motion in a sample of college age recreational males. Forty-two males (aged 18-24) healthy, physically active volunteers participated as subjects in this investigation and were randomly assigned to one of three groups (1 set of 20 seconds dynamic stretch, 1 set of 20 second static stretch, or control). The knee and hip range of motion, sit and reach, and jump height were measured before and after the treatment condition. The same measures were performed on the control group …

Challenging The Accuracy Of A Single-Test Lactate Threshold Protocol In Collegiate Rowers, Erica A. Soma, Michael M. Lockard, Stasinos Stavrianeas

International Journal of Exercise Science

Elite rowers use lactate threshold (LT) estimates as a basis for training intensity in order to achieve the greatest training volume. For convenience, LT is usually determined in a maximal LT/VO_2max test. This simultaneous test is problematic because it requires a large power increment, which may not give the most accurate LT. PURPOSE: To challenge the validity of a simultaneous LT/VO_2max test to estimate LT in rowers. METHODS: Collegiate rowers (n=20, 16F and 4M, age 19.3±1.3 years, height 171.5±7.1 cm, weight 70±14 kg, VO_2max 44.6±5.5 ml•kg^-1•min^-1) performed two LT tests. Participants completed an …

Racial/Ethnic Differences In Bone Mineral Density Of Young Adults, Linda D. Wilkin, Tristan D. Sims, Matthew C. Jackson, Bryan L. Haddock

International Journal of Exercise Science

An estimated 1.5 million people suffer a bone disease-related fracture every year. Most work investigating bone mineral density (BMD) focuses on post-menopausal females but a report from the Surgeon General in 2004 stated that of particular concern are men, racial and ethnic minorities, poor individuals, individuals with disabilities, and individuals living in rural areas. The purpose of this study was to examine the racial/ethnic differences in bone mineral density of young adults and to investigate any correlations with variables suggested to influence BMD. BMD was assessed at a younger age than most studies based on the assumption that osteoporosis is …

The Association Between Soft Drink Consumption And Body Fat In Females Age 16 To 24, Megan M. Costello, Kate A. Heelan, Deborah A. Mowry, Bryce Abbey

International Journal of Exercise Science

American soft drink consumption (SDC) has increased since the 1960’s surpassing all other kinds of beverage consumption. In recent years, the scientific literature has suggested that SDC has been linked to the rising epidemic of obesity in children and adolescents. However, there is lack of information in scientific literature on the effects of SDC on body fat (BF%) in young females. The purpose of this study was to determine the association between SDC and BF% in young women, ages 16-24 years. Sixty-six females were asked to complete a 3-day food record, food frequency questionnaire, and an assessment BF% by dual-energy …

An Investigation Of Lower-Extremity Functional Asymmetry For Non-Preferred Able-Bodied Walking Speeds, John Rice, Matthew K. Seeley

International Journal of Exercise Science

Functional asymmetry is an idea that is often used to explain documented bilateral asymmetries during able-bodied gait. Within this context, this idea suggests that the non-dominant and dominant legs, considered as whole entities, contribute asymmetrically to support and propulsion during walking. The degree of functional asymmetry may depend upon walking speed. The purpose of this study was to better understand a potential relationship between functional asymmetry and walking speed. We measured bilateral ground reaction forces (GRF) for 20 healthy subjects who walked at nine different speeds: preferred, +10%, +20%, +30%, +40%, -10%, -20%, -30%, and -40%. Contribution to support was …

Riding Position And Lumbar Spine Angle In Recreational Cyclists: A Pilot Study., Samantha J. Schulz, Susan J. Gordon

International Journal of Exercise Science

This pilot study investigated the reliability of an inclinometer to assess lumbar spine angle in three different cycling positions, and explored the relationship between lumbar spine angle and riding position, anthropometry, bike measures and low back pain (LBP).

Cyclists were recruited from two cycle clubs. Anthropometric variables and bike set-up were measured before participants’ bikes were secured in a wind trainer. Cyclists then adopted three positions for riding, upright on the handlebars, on the brake levers and on the drops, according to a random allocation. The angle of the lumbar spine was measured, using an inclinometer, at zero minutes and …

Type Of Encouragement Influences Peak Muscle Force In College-Age Women, Ruth M. Amagliani, John K. Petrella, Alan P. Jung

International Journal of Exercise Science

Purpose: To investigate if the type of encouragement during a maximal voluntary isometric contraction (MVIC) had an influence on peak muscle force in strength-trained versus untrained collegiate women. Methods: Eleven strength-trained (20.4 + 1 yr) and twelve untrained (20.8 + 1 yr) women participated in three, five-second MVICs of seated knee extension. The three trials consisted of verbal only encouragement, verbal + visual encouragement, and verbal + pain avoidance encouragement. In all three trials, the participants received the same verbal encouragement. Trials were counterbalanced to minimize any possible order effects. A repeated measure ANOVA was used to analyze data. Any …

Exercise Science Academic Programs And Research In The Philippines, Norberto Madrigal, Josephine Joy Reyes, Jeffrey Pagaduan, Reil Vinard Espino

International Journal of Exercise Science

In this invited editorial, professors from leading institutions in the Philippines, share information regarding their programs relating to Exercise Science. They have provided information on academic components such as entrance requirements, progression through programs, and professional opportunities available to students following completion; as well as details regarding funding available to students to participate in research, collaboration, and specific research interests.

Full Abstract Program

International Journal of Exercise Science: Conference Proceedings

2010 International e-Conference on Kinesiology and Integrated Physiology. Full Abstract Program.

The Expression Of Matrix Metalloproteinase-9 And -2 In Olfactory Injury And Recovery, Stephen Bakos

Theses and Dissertations

The olfactory system has the remarkable capacity for neurogenesis following injury. However, the molecular mechanisms important for reinnervation of the olfactory bulb (OB) remain unknown. The matrix metalloproteinases (MMPs) are important components in many central nervous system (CNS) injury paradigms, yet remain unexplored in olfactory injury and recovery. To address the role of MMPs, the temporal expressions of MMP-9 and MMP-2 were examined in 3 olfactory injury models: nerve transection (NTx), methyl bromide gas (MeBr) exposure, and nerve transection with Teflon barrier (NTx-TB). Each injury model represents a different degree of olfactory injury and neuronal recovery. In NTx, sensory axons …

Isoform Of Apoe With Retained Intron 3; Quantitation And Identification Of An Associated Single Nucleotide Polymorphism, Laura S. Dieter, Steven Estus

Physiology Faculty Publications

BACKGROUND: Alleles of apolipoprotein E (APOE) are the major genetic risk factor for late onset Alzheimer's Disease (LOAD). Recently, an APOE splice variant that retains intron 3 (APOE-I3) was identified. To gain insight into the possible role of this isoform in LOAD, we quantified its expression in a cohort of 56 human brain specimens by using quantitative RT-PCR.

RESULTS: We found that APOE-I3 generally represents a low percentage (< 0.5%) of overall APOE expression. However, in one specimen, the proportion of APOE-I3 was increased about ~13 fold. This specimen was unique in the cohort for possessing the minor allele of an intron 3 single nucleotide polymorphism (SNP), rs12982192. Additionally, an allelic expression imbalance study indicated that the rs12982192 minor allele was associated with increased APOE-I3 expression.

CONCLUSIONS: Overall, we interpret our results as suggesting that APOE-I3 represents a minor portion of APOE expression and that rs12982192 is associated with APOE intron 3 retention. Since the minor allele …

Association Between Chronic Liver And Colon Inflammation During The Development Of Murine Syngeneic Graft-Versus-Host Disease, Jason Anthony Brandon, Jacqueline Perez-Rodriguez, C. Darrell Jennings, Donald A. Cohen, Vishal J. Sindhava, Subbarao Bondada, Alan M. Kaplan, J. Scott Bryson

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The murine model of cyclosporine A (CsA)-induced syngeneic graft-versus-host disease (SGVHD) is a bone marrow (BM) transplantation model that develops chronic colon inflammation identical to other murine models of CD4⁺ T cell-mediated colitis. Interestingly, SGVHD animals develop chronic liver lesions that are similar to the early peribiliary inflammatory stages of clinical chronic liver disease, which is frequently associated with inflammatory bowel disease (IBD). Therefore, studies were initiated to investigate the chronic liver inflammation that develops in the SGVHD model. To induce SGVHD, mice were lethally irradiated, reconstituted with syngeneic BM, and treated with CsA. All of the SGVHD animals …

Experimental Models Of Brain Hemorrhage Using Clostridial Collagenase, Tim Lekic

Loma Linda University Electronic Theses, Dissertations & Projects

Stroke is the third leading cause of death and is the leading cause of disability in the United States, and the most severe form is known as intracerebral hemorrhage, which is bleeding into the brain. The goal of this study is to establish novel animal models to buildup foundations for translational research of intracerebral hemorrhage. We, for the first time, established four animal models, including cerebellum hemorrhage, pontine hemorrhage, neonatal matrix hemorrhage and maternal postpartum hemorrhage. Those models are established according to intracerebral hemorrhage patient subpopulations, and characterized the neurobehavioral and morphological outcomes. These studies have established the requisite for …

Mip/Mtmr14 And Muscle Aging, Scott K. Powers, Michael B. Reid

Physiology Faculty Publications

No abstract.

A Phenomenological Investigation Of Women's Experiences With Personal Training, Melissa N. Madeson, Cherilyn Hultquist, Amy Church, Leslee A. Fisher

International Journal of Exercise Science

Personal training is a rapidly growing industry in a country that is in dire need of physical fitness and health improvements. The purpose of this phenomenological study was to better understand women’s experiences with personal training. To address the research question, eight female participants ages 24 to 54 years were interviewed using the following phenomenological question: “When you think about your current experience with personal training what stands out for you?” The interviews were conducted, transcribed, and qualitatively analyzed to identify themes in participants’ responses. The ground that emerged was positive experience within which existed four figural themes: Relationships, trainer …