The Effect Of Temperature Extremes On Cardiorespiratory Function In The Grass Shrimp Palaemonetes Pugio: Oxygen Limited Thermal Tolerance,
2013
University of Nevada, Las Vegas
The Effect Of Temperature Extremes On Cardiorespiratory Function In The Grass Shrimp Palaemonetes Pugio: Oxygen Limited Thermal Tolerance, Teresa Mika
UNLV Theses, Dissertations, Professional Papers, and Capstones
Aquatic poikilothermic animals must either cope with or compensate for the mismatch in oxygen supply and demand present at high temperatures. Oxygen limited thermal tolerance explains how aerobic scope is limited by insufficient oxygen supply and sets the performance window in animals. This work explores the effects of temperature on the different components of the oxygen delivery system, in both normoxic and hyperoxic conditions to determine if supplying more oxygen to the system mitigates the effects of temperature effects on the cardiorespiratory system and extends oxygen limited thermal tolerance to higher temperatures. The effect of temperature and oxygen condition was …
Dexamethasone Rapidly Increases Gaba Release In The Dorsal Motor Nucleus Of The Vagus Via Retrograde Messenger-Mediated Enhancement Of Trpv1 Activity,
2013
University of Kentucky
Dexamethasone Rapidly Increases Gaba Release In The Dorsal Motor Nucleus Of The Vagus Via Retrograde Messenger-Mediated Enhancement Of Trpv1 Activity, Andrei V. Derbenev, Bret N. Smith
Physiology Faculty Publications
Glucocorticoids influence vagal parasympathetic output to the viscera via mechanisms that include modulation of neural circuitry in the dorsal vagal complex, a principal autonomic regulatory center. Glucocorticoids can modulate synaptic neurotransmitter release elsewhere in the brain by inducing release of retrograde signalling molecules. We tested the hypothesis that the glucocorticoid agonist dexamethasone (DEX) modulates GABA release in the rat dorsal motor nucleus of the vagus (DMV). Whole-cell patch-clamp recordings revealed that DEX (1-10 µM) rapidly (i.e. within three minutes) increased the frequency of tetrodotoxin-resistant, miniature IPSCs (mIPSCs) in 67% of DMV neurons recorded in acutely prepared slices. Glutamate-mediated mEPSCs were …
Direction Of Aminoacylated Transfer Rnas Into Antibiotic Synthesis And Peptidoglycan-Mediated Antibiotic Resistance,
2013
The Ohio State University
Direction Of Aminoacylated Transfer Rnas Into Antibiotic Synthesis And Peptidoglycan-Mediated Antibiotic Resistance, Jennifer Shepherd, Michael Ibba
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Prokaryotic aminoacylated‐transfer RNAs often need to be efficiently segregated between translation and other cellular biosynthetic pathways. Many clinically relevant bacteria, including Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa direct some aminoacylated‐tRNA species into peptidoglycan biosynthesis and/or membrane phospholipid modification. Subsequent indirect peptidoglycan cross‐linkage or change in membrane permeability is often a prerequisite for high‐level antibiotic resistance. In Streptomycetes, aminoacylated‐tRNA species are used for antibiotic synthesis as well as antibiotic resistance. The direction of coding aminoacylated‐tRNA molecules away from translation and into antibiotic resistance and synthesis pathways are discussed in this review.
Paired Interactions Between Kir Channels And Tertiapin-Q,
2013
Virginia Commonwealth University
Paired Interactions Between Kir Channels And Tertiapin-Q, Chul Ho Yang
Theses and Dissertations
Kir channels serve diverse and important roles throughout the human body and malfunctions of these channels are implicated in various channelopathies. Specific inhibitors for different subtypes of Kir channels are not available. However, Tertiapin-Q (TPNQ), a polypeptide isolated from honey bee venom, differentially inhibits certain subtypes of Kir channels with nanomolar affinity: ROMK1 (Kir1.1) and GIRK1/GIRK4 (Kir3.1/Kir3.4). Modification of TPNQ to increase selectivity for target channels bears great therapeutic potential. The in silico studies based on TPNQ-docked channel models, ROMK1_IRK2 (Kir1.1_Kir2.2) and GIRK2 (Kir3.2), predicted specific paired residue interactions and were experimentally validated here. In ROMK1 E123A mutant, the TPNQ …
Lipid Ii-Independent Trans Editing Of Mischarged Trnas By The Penicillin Resistance Factor Murm,
2013
The Ohio State University
Lipid Ii-Independent Trans Editing Of Mischarged Trnas By The Penicillin Resistance Factor Murm, Jennifer Shepherd, Michael Ibba
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Streptococcus pneumoniae is a causative agent of nosocomial infections such as pneumonia, meningitis, and septicemia. Penicillin resistance in S. pneumoniae depends in part upon MurM, an aminoacyl-tRNA ligase that attaches l-serine or l-alanine to the stem peptide lysine of Lipid II in cell wall peptidoglycan. To investigate the exact substrates the translation machinery provides MurM, quality control by alanyl-tRNA synthetase (AlaRS) was investigated. AlaRS mischarged serine and glycine to tRNAAla, as observed in other bacteria, and also transferred alanine, serine, and glycine to tRNAPhe. S. pneumoniae tRNAPhe has an unusual U4:C69 mismatch in its acceptor stem that …
Acquisition Delays Affect Lymphocyte Subset Counts But Not Markers Of Exercise-Induced Apoptosis,
2013
Western Kentucky University
Acquisition Delays Affect Lymphocyte Subset Counts But Not Markers Of Exercise-Induced Apoptosis, Holly B. Kell, Guilherme Borges Pereira, Ramires Alsamir Tibana, Michael Jarrett, Mark A. Schafer, Jonato Prestes, Antonio Santo, James W. Navalta
International Journal of Exercise Science
International Journal of Exercise Science 6(3) : 250-255, 2013. With the emergence of shared facilities, the possibility of a processing delay is increased as time on instrumentation becomes less available. The purpose of this investigation was the evaluate the effect of a 24h time delay on lymphocyte subset concentration, as well as for the apoptotic marker annexin V. Fourteen healthy individuals completed an incremental treadmill test to exhaustion, and blood samples were obtained before and after exercise. The samples were labeled in duplicate with mixtures consisting of flow cytometry staining buffer, the biomarker for early-phase cell death (annexin V), and …
Activity-Specific Effects Of Fatigue Protocols May Influence Landing Kinematics: A Pilot Study,
2013
Arizona State University
Activity-Specific Effects Of Fatigue Protocols May Influence Landing Kinematics: A Pilot Study, Adrian Chavez, Duane Knudson, Rod Harter, Kevin Mccurdy
International Journal of Exercise Science
International Journal of Exercise Science 6(3) : 242-249, 2013. Fatigue is a common neuromuscular factor examined in relation to risk of ACL injury. Unfortunately, variations between the protocols used to induce fatigue in studies examining this phenomenon may have contributed to reported inconsistencies in the effects of fatigue on movements with high-risk of ACL injury. In addition, the ecological validity of fatigue experienced as a result of protocols commonly administered in the experimental setting is unclear. The purpose of this study was to examine the ecological validity, using basketball competition as the criterion measure, of two fatigue protocols commonly used …
Manuscript Has Been Retracted,
2013
Gonzaga University
Manuscript Has Been Retracted, Michael Smith, Nicholas Hanson, Alexander Lucas, Steven Devor
International Journal of Exercise Science
International Journal of Exercise Science 6(3) : 236-241, 2013. Manuscript has been retracted.
Children And Pedometers: A Study In Reactivity And Knowledge,
2013
University of Utah
Children And Pedometers: A Study In Reactivity And Knowledge, Steven L. Prewitt, James C. Hannon, Timothy A. Brusseau
International Journal of Exercise Science
International Journal of Exercise Science 6(3) : 230-235, 2013. Pedometers are simple inexpensive tools that are widely used for the measurement of physical activity. Reactivity is always a concern when using pedometers. An issue not yet addressed is the role previous knowledge has on the impact of reactivity. The primary purpose of this study was to examine if reactivity exists in 4th-6th grade students and if prior knowledge about pedometers had an effect on reactivity. Participants included 109 (54 females) 4th- 6th grade students from a suburban charter school. Prior to data collection, students …
Measuring Activity Energy Expenditure: Accuracy Of The Gt3x+ And Actiheart Monitors,
2013
University of Aberdeen
Measuring Activity Energy Expenditure: Accuracy Of The Gt3x+ And Actiheart Monitors, David Mcminn, Rijan Acharya, David A. Rowe, Stuart R. Gray, Julia L. Allan
International Journal of Exercise Science
International Journal of Exercise Science 6(3) : 217-229, 2013. The purpose was to determine the accuracy of the GT3X+ and Actiheart monitors for estimating energy expenditure (EE) and steps. Additionally, to investigate agreement between waist- and wrist-mounted GT3X+ EE outputs. Nineteen participants (mean age=30) completed three treadmill walking trials at self-selected slow, medium, and fast speeds while wearing two GT3X+ (waist and wrist) and an Actiheart. Activity monitor EE was compared to indirect calorimetry criterion EE using Pearson correlations and ANOVAs. A Bland-Altman plot was used to investigate agreement between GT3X+ waist- and wrist-determined EE. GT3X+ determined steps were compared …
Preseason And Post Season Body Composition Does Not Change Relative To Playing Time In Division I Female Basketball Players,
2013
University of South Dakota
Preseason And Post Season Body Composition Does Not Change Relative To Playing Time In Division I Female Basketball Players, Emma Ladwig, Andrew L. Shim, Jae P. Yom, Patrick Cross, Justin Beebe
International Journal of Exercise Science
International Journal of Exercise Science 6(3) : 208-216, 2013. The purpose of the study was to determine if preseason and postseason body fat percentages (BF%) change relative to playing time in Division I women’s basketball players. Subjects for the study included 11 National Collegiate Athletic Association (NCAA) Division I female collegiate basketball athletes over the age of 18 from a Midwest public university. Demographic data of each subject (age 20.09 +1.81 yrs., weight 71.13 +10.85 kg., height 176.48 + 8.33 cm.) was taken before the initial body fat assessment. The subjects underwent preseason and postseason BOD POD® testing to …
Measurements Of Heart Rate And Accelerometry To Determine The Physical Activity Level In Boys Playing Paintball,
2013
University of Nebraska Medical Center
Measurements Of Heart Rate And Accelerometry To Determine The Physical Activity Level In Boys Playing Paintball, Michelle Jarvi, Gregory A. Brown, Brandon S. Shaw, Ina Shaw
International Journal of Exercise Science
International Journal of Exercise Science 6(3) : 199-207, 2013. Paintball is a popular recreational sport played by 3.655 million Americans and may be sufficient physical activity to promote health. Paintball has been played as an organized sport since the 1980’s and is essentially a game of tag, except instead of touching an opponent by hand opponents are tagged by shooting them with a paintball that leaves a mark indicating who has been eliminated. A previous evaluation of paintball as physical activity had 13 subjects undergo a VO2max test to develop a heart rate (HR) /oxygen consumption relationship, and …
Evaluation Of A Circumference-Based Prediction Equation To Assess Body Composition Changes In Men,
2013
Pennington Biomedical Research Center
Evaluation Of A Circumference-Based Prediction Equation To Assess Body Composition Changes In Men, John M. Schuna Jr, Sarah J. Hilgers-Greterman, Trista L. Manikowske, Jared M. Tucker, Gary Liguori
International Journal of Exercise Science
International Journal of Exercise Science 6(3) : 188-198, 2013. This study evaluated the validity of the current U.S. Department of Defense (DOD) circumference-based prediction equation for males to detect body composition changes in comparison to air-displacement plethysmography (ADP). Body composition was assessed using ADP and the DOD equation at the beginning and end of an academic school year among 21 male (18-29 years-old) Army ROTC cadets. Body mass significantly increased (+1.8 Kg) after 9 months. Significant method by time interactions for percent body fat (percent body fat), fat mass (FM), and fat-free mass were found (p = 0.022, p …
The Effect Of Active Vs. Supine Recovery On Heart Rate, Power Output, And Recovery Time,
2013
Samford University
The Effect Of Active Vs. Supine Recovery On Heart Rate, Power Output, And Recovery Time, Lars M. Larson, Ryan M. Smeltzer, John K. Petrella, Alan P. Jung
International Journal of Exercise Science
International Journal of Exercise Science 6(3) : 180-187, 2013. The purpose of this research was to compare the effectiveness of two different post-exercise recovery methods, active and passive, on the heart rate (HR), peak power (PP), average power (AP) and time to Baseline Active Heart Rate (BAHR) following three short (10 s) bicycle sprints. Fourteen males (mean age: 21.0±0.7 yrs) participated in the study. Each participant performed two separate trials that included three maximal Wingate rides of 10 s each. In one trial each ride was followed by a two-minute supine recovery. In the second trial each ride was followed …
Down's Syndrome, Neroinflammation, And Alzheimer Neuropathogenesis,
2013
University of Kentucky
Down's Syndrome, Neroinflammation, And Alzheimer Neuropathogenesis, Donna M. Wilcock, W. Sue T. Griffin
Physiology Faculty Publications
Down syndrome (DS) is the result of triplication of chromosome 21 (trisomy 21) and is the prevailing cause of mental retardation. In addition to the mental deficiencies and physical anomalies noted at birth, triplication of chromosome 21 gene products results in the neuropathological and cognitive changes of Alzheimer's disease (AD). Mapping of the gene that encodes the precursor protein (APP) of the β-amyloid (Aβ) present in the Aβ plaques in both AD and DS to chromosome 21 was strong evidence that this chromosome 21 gene product was a principal neuropathogenic culprit in AD as well as DS. The discovery of …
Beta-Amyloid Inhibition Of Alpha 7 Nicotinic Acetylcholine Receptors And Factors That Potentially Influence The Aî²/Nachr Interaction,
2013
Brigham Young University - Provo
Beta-Amyloid Inhibition Of Alpha 7 Nicotinic Acetylcholine Receptors And Factors That Potentially Influence The Aî²/Nachr Interaction, Christopher L. Jacobsen
Theses and Dissertations
Alzheimer's disease (AD) is a neurodegenerative disorder that manifests in the form of deficiencies in cognitive processes such as memory and learning. The pathological features of AD include hyperphosphorylated tau proteins that form neurofibrillary tangles as well as senile plaques composed primarily of the peptide β-amyloid (Aβ). When present in high concentrations in the brain, Aβ inhibits certain subtypes of neuronal nicotinic acetylcholine receptors (nAChRs) in the hippocampus. The effects of Aβ in the hippocampus have proven to be neurotoxic, resulting in reduced functionality of nAChRs and the subsequent death of neurons in the cholinergic pathway. The early stages of …
Susceptibility Of Apoptotic Cells To Hydrolysis By Spla2: Molecular Basis And Mechanisms Defined,
2013
Brigham Young University - Provo
Susceptibility Of Apoptotic Cells To Hydrolysis By Spla2: Molecular Basis And Mechanisms Defined, Elizabeth Gibbons
Theses and Dissertations
Secretory phospholipase A2 hydrolyzes phospholipids at a lipid-water interface, resulting in pro-inflammatory products being released from cell membranes. Healthy cells are resistant to cleavage by this enzyme, but apoptotic cells become susceptible to its activity. Only bilayers with certain characteristics are able to be hydrolyzed. Most recently, studies in this lab have emphasized the idea that the biophysical state of the bilayer (in terms of lipid order, spacing, and fluidity) is relevant in determining the probability of one phospholipid escaping the membrane to be hydrolyzed. Prior to this study, it had been shown that apoptotic cells undergo biophysical alterations that …
S3-S4 Linker Modulation Of Voltage Sensor Relaxation In Voltage Gated Potassium Channels,
2013
Virginia Commonwealth University
S3-S4 Linker Modulation Of Voltage Sensor Relaxation In Voltage Gated Potassium Channels, W. Everett Fox
Theses and Dissertations
It is commonly assumed that the voltage dependence for activation of voltage-gated channels is an intrinsic characteristic of the protein that remains unchanged during electrical activity. However, sporadic reports have suggested otherwise by showing that voltage dependence changes in a use-dependent manner resulting in a voltage dependence shift towards negative potentials (Bezanilla et al., 1982; Bruening-Wright and Larsson, 2007; Kuzmenkin et al., 2004; Labro et al., 2012; Larsson and Elinder, 2000; Olcese et al., 1997; Piper et al., 2003; Shirokov et al., 1992). Although the mechanism underlying the shift in voltage dependence remains unclear, this process seems to have two …
The Effects Of Gain Of Function Mutant P53 And P63 On Eps8 And Cxcl5 Expression In Head And Neck Squamous Cell Carcinoma,
2013
Virginia Commonwealth University
The Effects Of Gain Of Function Mutant P53 And P63 On Eps8 And Cxcl5 Expression In Head And Neck Squamous Cell Carcinoma, Rubana Masood
Theses and Dissertations
Head and neck squamous cell carcinoma (HNSCC) is one of the ten most common cancers worldwide, with a survival rate of less than 50%. A class of mutant p53, known as gain of function (GOF) mutant p53, has been found to be expressed in tumors in these patients. GOF mutant p53 not only loses the wild type tumor suppressor functions, but also gains aberrant functions that have been linked to tumorigenesis. In this current study, we utilized a model system consisting of cells derived from HNSCC tumors in order to investigate our hypothesis that GOF mutant p53 enhances, and p63 …
Review Of Ourselves Unborn: A History Of The Fetus In Modern America, By Sara Dubow,
2013
University of Nebraska - Lincoln
Review Of Ourselves Unborn: A History Of The Fetus In Modern America, By Sara Dubow, Rose Holz
Women's and Gender Studies Program: Faculty Publications
With the publication of the Bancroft Prize-winning Ourselves Unborn, Sara Dubow offers a long overdue analysis and historicization of what has become a central feature in battles over reproductive rights: the fetus. Drawing upon legal and legislative records as well as educational tracts, museum exhibits, medical textbooks and journals, personal memoirs, and the popular press, Dubow traces what she calls “fetal stories” (4) in America from the late nineteenth century through the early twenty-first. In so doing, she persuasively reminds her readers the following: First, that our understanding of the fetus is not simply a product of biology or theology. …
