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A Fine Structure Comparison Of Three Species Of Argulus (Crustacea: Branchiura) Ectoparasites Of Farmed And Wild Fish, Adam Bowen, Richard A. Heckmann 2013 Brigham Young University

A Fine Structure Comparison Of Three Species Of Argulus (Crustacea: Branchiura) Ectoparasites Of Farmed And Wild Fish, Adam Bowen, Richard A. Heckmann

Journal of Undergraduate Research

Argulus, commonly called fish-lice, are crustacean ectoparasites of fish. This study compared three members of the genus Argulus: A. pugetensis from Nanaimo, British Columbia; A. coregoni from Curitiba, Brazil; and A. siamensis from Bangalore, India. It was hypothesized that argulids of different taxons must have morphological variations of commonly held structures. For example, two species of Argulus possess marginal membranes on their suckers, but the membranes should have different quantities and qualities of substructures. To test this hypothesis, all specimens were photographed with the JEOL 6100 scanning electron microscope; the micrographs of A. pugetensis and A. siamensis are the first …


Histological And Ultrastructural Evidence Of Osteoarthritis In Mice Carrying A Semi-Dominant Mutation Of Col2a1, Victoria Ryder, Dr. Robert Seegmiller 2013 Brigham Young University

Histological And Ultrastructural Evidence Of Osteoarthritis In Mice Carrying A Semi-Dominant Mutation Of Col2a1, Victoria Ryder, Dr. Robert Seegmiller

Journal of Undergraduate Research

Mice heterozygous for the semi-dominant mutation disproportionate micromelia (Dmm) express mild chondrodysplasia by three weeks postnatally. This phenotype is attributed to a deletion of three contiguous nucleotides within the Col2A1 gene on mouse chromosome 15. The phenotype of Dmm/+ mice resembles that of human Stickler syndrome, which can be caused by mutations in the human COL2A1 gene.


The Structural And Functional Characterization Of Cef-5 And Snk, Donna Melinda Earl Ashton, Dr. William S. Bradshaw 2013 Brigham Young University

The Structural And Functional Characterization Of Cef-5 And Snk, Donna Melinda Earl Ashton, Dr. William S. Bradshaw

Journal of Undergraduate Research

The malignant transformation of chicken embryo fibroblasts (CEF) following infection with the Rous sarcoma virus is mediated through the oncogene v-src. One of the first responses of infected cells is the transcriptional activation of a set of immediate-early genes. Among these are CEF-5 (homologue of EGRI, early growth response, in mammals) and serum induced kinase (snk).


Techniques And Analysis Of Mitochondrial Dna Of Local Populations Of Cottusbairdi, Amara Earley Bray, Dr. Dennis K. Shiozawa 2013 Brigham Young University

Techniques And Analysis Of Mitochondrial Dna Of Local Populations Of Cottusbairdi, Amara Earley Bray, Dr. Dennis K. Shiozawa

Journal of Undergraduate Research

Recent studies of native trout in Utah have found that populations in separate stream drainages tend to have unique mitochondrial DNA lineages. The association of lineage with drainage implies that the trout tend to remain within their own streams and do not readily migrate to adjacent streams.


The Locating Of Col11a2 Cell-Specific Enhancers, Jennifer L. Potter, Dr. Laura C. Bridgewater 2013 Brigham Young University

The Locating Of Col11a2 Cell-Specific Enhancers, Jennifer L. Potter, Dr. Laura C. Bridgewater

Journal of Undergraduate Research

The human skeletal structure is formed first by cartilage which later ossifies to bone. It follows, therefore, that if cartilage formation is aberrant, the resulting bone formation will be affected. Cartilage is composed of fibrils consisting of three types of collagen: type II, type IX, and type XI. These fibrils are only produced by chondrocyte (cartilage) cells.


Construction Of A Col11a1 Transgene And The Study Of The Col11a1 Mutation In Mice, Dana L. Murphy, Dr. Laura C. Bridgewater 2013 Brigham Young University

Construction Of A Col11a1 Transgene And The Study Of The Col11a1 Mutation In Mice, Dana L. Murphy, Dr. Laura C. Bridgewater

Journal of Undergraduate Research

Collagen fibrils play an important role in proper skeletal development. Defective collagen can cause human skeletal disorders such as osteoarthritis that affects an estimated 20.7 million Americans. A particular mouse line, housed here at BYU, contains a mutation in the Col11a1 gene. This mutation results in defective type XI collagen, which is an important component of articular cartilage, and eventually leads to osteoarthritis (1).


Angiotensin Ii-Mediated Switching Of Corticotropin-Releasing Hormone To Arginine Vasopressin In The Paraventricular Nucleus Under Chronic Stress Conditions, Jason T. Rich, Dr. James D. Porter 2013 Brigham Young University

Angiotensin Ii-Mediated Switching Of Corticotropin-Releasing Hormone To Arginine Vasopressin In The Paraventricular Nucleus Under Chronic Stress Conditions, Jason T. Rich, Dr. James D. Porter

Journal of Undergraduate Research

The purpose of my study was to quantify the effects of angiotensin II on young rats under extreme stress. We hypothesized that we would observe an angiotensin II-mediated switch in brain hormonal expression from corticotropin-releasing hormone (CRH) to arginine vasopressin (AVP). Ten rats were equipped with osmotic ventricular pumps. Five rats received losartan (an angiotensin II antagonist) and the other five received a saline solution. Three rats where left untreated, to act as a control for the experiment. Two losartan treated rats and one saline treated rat died during the stressing period. However the losartan pumps were salvaged and implanted …


Proton Conductance Through The Membrane Protein Gramicidin M, Jason D. Merrell, David D. Busath 2013 Brigham Young University

Proton Conductance Through The Membrane Protein Gramicidin M, Jason D. Merrell, David D. Busath

Journal of Undergraduate Research

After discussion with my faculty mentor, together we decided that my original ORCA proposal would not best suit the needs of the laboratory in which I am allowed to work. Upon reaching this conclusion, a new research plan was organized. The following outlines the progress of this new plan and demonstrates the on-going research that was facilitated via my ORCA scholarship.


Effect Of Phytoestrogens On Brain Development And Function, Jacob C. Ong, Dr. Edwin D. Lephart 2013 Brigham Young University

Effect Of Phytoestrogens On Brain Development And Function, Jacob C. Ong, Dr. Edwin D. Lephart

Journal of Undergraduate Research

Phytoestrogens are estrogen-like compounds derived from plants such as the soy plant and can mimic the function of the human estrogen hormone by binding to estrogen receptors. Estrogen plays a major role in the prevention of osteoperosis and cardiovascular disease in woman. Evidence also suggests that estrogen influences various parts of the memory process and that its impact on an organism is dependent on the developmental stage at which exposure occurs [1].


Determining Homology Of Turkey Round Heart Disease Marker Sequences: A Computer-Based Study, Ryan D. McAllister, Dr. David Kooyman 2013 Brigham Young University

Determining Homology Of Turkey Round Heart Disease Marker Sequences: A Computer-Based Study, Ryan D. Mcallister, Dr. David Kooyman

Journal of Undergraduate Research

Round heart disease (RHD) is a spontaneous occurring cardiomyopathy in turkeys. The domestic turkey Meleagris gallopavo is affected by low body weight and premature death due to congestive heart failure from RHD (1). RHD results in decreased profitability for the animal agriculturists and higher prices for turkey products. DNA markers have been identified for both normal turkeys and for turkeys affected by RHD. These genetic markers have been amplified using Random Amplified Polymorphic DNA (RAPD) analysis (2). 16 DNA marker sequences amplified by RAPD have now been determined (3). Basic Local Alignment Search Tool (BLAST) is a computer program designed …


Comparison Of Gramicidin A And Gramicidin M Channel Properties In Kcl, Nacl, And Hcl, Jeffrey C. Markham, David D. Busath 2013 Brigham Young University

Comparison Of Gramicidin A And Gramicidin M Channel Properties In Kcl, Nacl, And Hcl, Jeffrey C. Markham, David D. Busath

Journal of Undergraduate Research

The oligopeptide gramicidin is one of the simplest proteins which can form channels in cell membranes to conduct ions into or out of a cell. The peptide is produced by the bacteria Bacillus brevis. It can also be synthesized by scientists for research purposes. By producing slightly modified variants of the standard gramicidin structure, conclusions can be drawn about structurefunction relationships based on experimentation with the variants.


Walking Stick (Insect: Phasmida) Phylogeny And The Evolution Of Insect Flight Based On Molecular Data, Taylor J. Maxwell, Dr. Michael F. Whiting 2013 Brigham Young University

Walking Stick (Insect: Phasmida) Phylogeny And The Evolution Of Insect Flight Based On Molecular Data, Taylor J. Maxwell, Dr. Michael F. Whiting

Journal of Undergraduate Research

The evolution and development of wings and flight has fascinated scientists for decades. From our research we happened upon an exciting discovery concerning flight in the phylogeny of walking sticks. A basic phylogeny was to be reconstructed from molecular data with the purpose of understanding the classification of the group. The phylogeny enabled us to discover the initial loss of wings and flight in phasmid history and the consequent gain of flight in later lineages. We are not aware of any other organism, which has lost and subsequently regained such a complex character.


Non-Equilibrium Molecular Dynamics Study Of An Ion Channel With Aqueous Nacl: A Slab Geometry Approach, Nathan B. Holladay, David D. Busath 2013 Brigham Young University

Non-Equilibrium Molecular Dynamics Study Of An Ion Channel With Aqueous Nacl: A Slab Geometry Approach, Nathan B. Holladay, David D. Busath

Journal of Undergraduate Research

An ion channel is a tunnel that allows ions to cross a barrier. Ion channels are common components of cellular membranes, helping to regulate ionic concentrations in different areas inside or outside cells. Electrical and/or concentration differences between one side of a membrane and the other often drive the net flow of ions in one direction or the other across the membrane. Theoretical biophysicists have used computer-based molecular dynamics simulations for years now to study the behavior of ions within ion channels under equilibrium conditions. Before our study, however, few or no studies had been done in which molecular dynamics …


Increasing Bilayer Membrance Fusion Rates By Increasing Cholesterol Content, David Lee, Dr. Dixon Woodbury 2013 Brigham Young University

Increasing Bilayer Membrance Fusion Rates By Increasing Cholesterol Content, David Lee, Dr. Dixon Woodbury

Journal of Undergraduate Research

A bilayer is a two-layered membrane made of lipids that form around cells. These membranes are formed by hydrophobic tails (water fearing) and hydrophilic heads (water loving) that align such that the tails are isolated from water, while the heads are exposed to water to form micelles, liposomes, or sheets (Fig. 1). They are used to regulate what can enter and exit a cell. A fusion event occurs when a liposome fuses with a sheet. In our experiments, we are able to see the effects on fusion rate by altering the cholesterol content of the sheets.


Colour And Odour Drive Fruit Selection And Seed Dispersal By Mouse Lemurs, Kim Valenta, Ryan J. Burke, Sarah A. Styler, Derek A. Jackson, Amanda D. Melin, Shawn M. Lehman 2013 University of Toronto

Colour And Odour Drive Fruit Selection And Seed Dispersal By Mouse Lemurs, Kim Valenta, Ryan J. Burke, Sarah A. Styler, Derek A. Jackson, Amanda D. Melin, Shawn M. Lehman

Dartmouth Scholarship

Animals and fruiting plants are involved in a complex set of interactions, with animals relying on fruiting trees as food resources, and fruiting trees relying on animals for seed dispersal. This interdependence shapes fruit signals such as colour and odour, to increase fruit detectability, and animal sensory systems, such as colour vision and olfaction to facilitate food identification and selection. Despite the ecological and evolutionary importance of plant-animal interactions for shaping animal sensory adaptations and plant characteristics, the details of the relationship are poorly understood. Here we examine the role of fruit chromaticity, luminance and odour on seed dispersal by …


Phytoestrogens: Effects On Male Sexual Health Parameters, Trevor Hansen, Dr. Edwin Lephart 2013 Brigham Young University

Phytoestrogens: Effects On Male Sexual Health Parameters, Trevor Hansen, Dr. Edwin Lephart

Journal of Undergraduate Research

Phytoestrogens are estrogen-like molecules derived from soy-based foodstuffs such as soybeans, tofu and even certain cereals. In particular, our study focused on a subclass of phytoestrogens called isoflavones. Of the many isoflavones, daidzene and genistein are thought to exert the most potent estrogenic hormone activity, and thus have been the subject of much recent research.


The Role Of Rage In Inflammatory Lung Disease Induced By Diesel Particulate Matter, Karisa Wasley, Dr. Paul Reynolds 2013 Brigham Young University

The Role Of Rage In Inflammatory Lung Disease Induced By Diesel Particulate Matter, Karisa Wasley, Dr. Paul Reynolds

Journal of Undergraduate Research

The causes of high morbidity and mortality associated with inflammatory respiratory diseases are not well understood. My research project helped to shed light on cellular signaling pathways associated with inflammatory disease, particularly those caused by or worsened by air pollutants. Asthmatics, in particular, have a difficult time dealing with common pollutants found in cities, including diesel particulate matter (PM). I focused specifically on the inflammation induced by air pollutants such as diesel PM and the activation of signaling pathways initiated by their binding with the receptors for advanced glycation end-products (RAGE). RAGE is involved developmentally in the lungs and specifically …


There’S No Wrong Way For A Cell To Die: Caspase-Independent Programmed Cell Death Pathways Resulting In Apoptotic Characteristics, Katalyn Pickett, Dr. John D. Bell 2013 Brigham Young University

There’S No Wrong Way For A Cell To Die: Caspase-Independent Programmed Cell Death Pathways Resulting In Apoptotic Characteristics, Katalyn Pickett, Dr. John D. Bell

Journal of Undergraduate Research

There are a number of ways that cells can die. One way is through apoptosis, also known as programmed cell death. This death pathway is characterized by orderly signals and mechanisms that essentially tell the cell to die without “making a mess”. Necrosis, another form of cell death, can be thought of as cell “homicide”; cells, generally in response to some environmental stress, are simply unable to survive and therefore die quickly and seemingly without regulation. Previously, the line between apoptosis and necrosis was thought to be distinct, but recent studies have shown that there are cell death pathways that …


Destabilization Of The Medial Meniscus: A Non-Genetic Approach To Inducing Osteoarthritis In Mice, Christopher Stockdale, Joshua Lloyd, Dr. Robert E. Seegiller 2013 Brigham Young University

Destabilization Of The Medial Meniscus: A Non-Genetic Approach To Inducing Osteoarthritis In Mice, Christopher Stockdale, Joshua Lloyd, Dr. Robert E. Seegiller

Journal of Undergraduate Research

Osteoarthritis (OA) is a common disease that causes discomfort and pain due to the degradation of the articular cartilage found on the surface of bones within the body’s joints. A properly functioning joint has a strong network of articular cartilage that can handle the constant strain caused by the use and movement of the joints. Individuals who suffer with OA have a compromised cartilage network that can result in softening of the cartilage, rough joint surfaces, fissures in the cartilage, and eventually direct bone on bone contact within the joint.


A Study Of Biological Photonic Crystals In The Effects Of Beta Amyloid On Nicotinic Acetylcholine Receptors In Alzheimer’S: Internal Opal Scale Structures Of Iridescent Beetle Species In The Cerambycidae Family, Andrew Romney, Dr. Sterling Sudweeks 2013 Brigham Young University

A Study Of Biological Photonic Crystals In The Effects Of Beta Amyloid On Nicotinic Acetylcholine Receptors In Alzheimer’S: Internal Opal Scale Structures Of Iridescent Beetle Species In The Cerambycidae Family, Andrew Romney, Dr. Sterling Sudweeks

Journal of Undergraduate Research

For the past two years, I have worked in Dr. Sterling Sudweeks’ research lab experimenting with neurotransmitter receptors that are implicated in the development of Alzheimer’s Disease. When functioning normally, these receptors act as ion channels which open upon binding nicotine or acetylcholine and are thus appropriately named nicotinic acetylcholine receptors (nAcRs). nAcRs’s are found at the neuromuscular junction as well as in nervous tissue and are comprised of 5 subunits. Dr. Sudweeks’ previous research suggests that the alpha 3 and beta 2 subunits are the most common subunits in receptors in the hippocampus, which is the primary area of …


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