Physiology Commons^™

Effect Of Eleven Weeks Training Program On Inflammatory, Hematological And Biochemical Parameters In Rat Skeletal Muscle, Liver And Serum, Fernando Oliveira Catanho Da Silva, Rejane Lucco, Rodrigo Ferraresso, Danilo Lopes Ferrucci

International Journal of Exercise Science: Conference Proceedings

Physical exercise is known causer of acute traumas in biological structures. Chronically, physical training or can lead to a coherent sequence of traumas leading to Adaptation (Functional Overreaching – FOR) or to an imbalance between stimulus and recovery leading to Overtraining Syndrome (OTS). In the present study we analyzed muscle and hepatic cytokines concentration: Interleukin-6 (IL6), Tumor Necrose Factor-Alpha (TNF-α), Interleukin-1Beta (IL1-β) and Interleukin-10 (IL-10); muscle and serum concentration of glutamate (GLU) and glutamine (GLN); serum concentration of C-reactive Protein (CRP), Alpha-1 Glicoprotein Acid (AGA), Albumin (ALB), Uric Acid (UA), Ferric Reducing Ability of Plasma (FRAP), Creatine Kinase (CK), Red …

Effect Of An Endurance Training-Overtraining Protocol On Rat Muscular Oxidative Capacity, Rodrigo Luiz Perroni Ferraresso, Denise Vaz De Macedo, Rodrigo Hohl

International Journal of Exercise Science: Conference Proceedings

Overtraining may outcomes for functional overreaching (FOR), a short term decline in performance that leads eventually to an improvement in performance after recovery, or nonfunctional overreaching (NFOR) when performance decline may be reversed only by a longer regenerative period. Recently we developed a training-overtraining protocol for rats with increased workload: eight weeks of daily exercise sessions, followed by three weeks of increasing daily training frequency (2, 3 and 4 times) with decreasing recovery time between sessions (4, 3 and 2h), characterized by analyses of performance before training (T1) and after the 4th(T2), 8th(T3), 9th(T4), 10th(T5) and 11th(T6) training weeks. All …

Determination Of Blood Glucose Threshold In Boys: Descriptive Analysis, Alex Garcia, Jorge Luis Domingues Honório, Runer Augusto Marson, Paulo Henrique Silva Marques De Azevedo

International Journal of Exercise Science: Conference Proceedings

The blood glucose threshold (GT) has been used for the assessment of the aerobic capacity for trained individuals in replace of the blood lactate and ventilatory parameters for anaerobic threshold determination. But, there are no studies with boys. The purpose of this study was to measure the intensity corresponding to the GT in a group of boys. Eight boys (11±1.12 years; 38±6.93 kg; 1.44±0.09 m; 18±1.79 kg/m2) performed a graded maximal exercise test on a cycloergometer to determine the Watts peak (WP), heart rate maximum (HRmax), Watts at GT (GTw) and heart rate at GT (GThr). The initial intensity was …

Comparison Of The Effects Of Different Anesthetics On Serum Haemolysis And Glycogenolysis In Rats, Eduarda F. A. Machado, Ana Carolina R. Normand, Lázaro A. S. Nunes, Denise V. Macedo

International Journal of Exercise Science: Conference Proceedings

The anesthetics can differently affect the structure and biological function of tissues and systems. The objective of this study was to compare the effects of three injectable anesthetics generally used in experiments with animals on the degree of haemolysis and glycogenolysis after deep anesthesia. 20 Wistar male rats (330-440g) were used. The animals were divided into three groups: Cloral Hydrate (CH), Ketamine + Xylazine (KX), Zoletil 50® (zolazepam and tiletamina) + Xylazine (ZTX). After deep anesthesia the total blood was collected. Immediately the liver, the white and red gastrocnemius muscles were also withdrawn. The degree of serum haemolysis was quantified …

Comparison Of Basal Metabolic Rate By Indirect Calorimetry And Predictive Model, Tatienne Neder F. Da Costa, Mateus Moraes Domingos, Patrícia Brassolatti, Shislene Batistela Tokuda

International Journal of Exercise Science: Conference Proceedings

The objective of this study was to compare the values of basal metabolic rate (BMR) obtained by indirect calorimetry (IC) and by predictive model (PM). Methodology: 6 women, age (23.8 ± 3.1 years), height (165 ± 5.8 cm), body mass (55.9 ± 4.9 kg), fat free mass (43 ± 2.1 kg), fat body mass (12.9 ± 3.3 kg), percentage of body fat (22.8 ± 4.2%) were subjected to the test of IC. The IC test was done early in the morning, after 12 hours of fasting, without attainment of physical activity within the last 24 hours and abstinence of alcohol …

Comparative Analysis Of The Energy Expenditure In Walking And The Running In Young Men, Lucas De Castro Cardoso, Marcelo De Castro Cesar, Pamela Roberta Gomes Gonelli, Claudio Alexandre Gobatto

International Journal of Exercise Science: Conference Proceedings

Walking below 6 km/h and running above 8 km/h are efficient effort intensities to maintain the economy of energy expenditure in the mechanical work. However, in the intermediate range (between the transition of the walking to running), the mechanical work against the energy expenditure still requires analysis. So, the purpose of the present study was to compare the energy expenditure between walking and running in the load work immediately below of the inversion point in the caloric expenditure. Ten young male subjects participated of the study (24.2 ± 2.04 years; 180.7 ± 3.8 cm; 79.5 ± 8.6 kg). The transition …

Comparative Study Of Injury Incidence In Soccer Players During Regional Games Of Sertãozinho – 2006 And Jaboticabal – 2008, Luiz Fernando Approbato Selistre, Luciano Maia Alves Ferreira, Oswaldo Stamato Taube, Edson Alves Barros Jr.

International Journal of Exercise Science: Conference Proceedings

Soccer is one of the most popular and practiced sports in the world. This sport has been changed over the years and instead of emphasis on technique, currently the physical components are more favored, increasing the injury risk. Regional Games were created to stimulate young athletes and sports practice. The aim of this study was to perform a comparative epidemiological survey of male sub-21 soccer players injuries in the Regional Games of 2006 and 2008. This is an epidemiological and comparative study of analytical type. Records of assessment used at Regional Games of Sertãozinho 2006 and Jaboticabal 2008 were consulted. …

Caffeine Supplementation And Intermittent Exercise: Effects On White Blood Cells, Marco Machado, Felipe Sampaio- Jorge, Juliano G. Barreto, Rodrigo A. Lazo-Osorio

International Journal of Exercise Science: Conference Proceedings

Caffeine is a common substance in the diets of most athletes and it is appearing in energy drinks, sport gels, alcoholic beverages and diet aids. The effects of caffeine ingestion prior to endurance training have been extensively studied. However, less is known concerning the effects of caffeine ingestion prior to intermittent exercise. The purpose of this study was to evaluate the effect of caffeine ingestion prior to intermittent exercise on white blood cells in soccer players. 15 professional soccer players completed a double-blind, placebo-controlled experiment. Forty-five minutes before exercise all subjects received 5.5 mg.kg-1 of caffeine (E+C, n=8) or placebo …

Behavior Of Bicarbonate Ion Between Capillary Blood And Saliva During Acute Exercise, Alexandre Fonseca Brandão, Roberto Mario Machado Verzola, Heloisa Sobreiro Selistre-De- Araujo, Rafael Nativio

International Journal of Exercise Science: Conference Proceedings

This study includes the monitoring of the behavior of the bicarbonate ions concentration in the capillary blood and in saliva, with the main objective of establishing reliable parameters of the relationship of this variable between these two fluid compartments. The tests were conducted under a modified Balk protocol in the cycloergometer with progressively increasing load of 50 to 50 watts and measurement of bicarbonate ion was held in a gas analyzer ABL 800 FLEX Radiometer, assigned by UNIMED of São Carlos. It was necessary to develop 70 glass capillary 110μL and one adapter to collect saliva, manufactured in PVC. The …

Aquatic Plyometric Training Associated With Androgenic Anabolic Steroids Do Not Increase Muscle Mass In Rats, Uliana Sbeguen Stotzer, Rita De Cássia Marqueti, Milena De Moura Paschoal, Heloisa Sobreiro Selistre De Araújo

International Journal of Exercise Science: Conference Proceedings

To improve athletic performance or for aesthetic reasons, athletes and no athletes may use androgenic anabolic steroids (AAS). Numerous studies have reported that aquatic plyometric training (APT) can improve muscular strength and vertical jump; however the effect of these training protocols on muscular mass are poorly investigated. The aim of this study was to investigate the effects of APT associated with AAS in the soleus, gastrocnemius (Gastro), extensor digitorium longus (EDL) and tibialis anterior (TA) skeletal muscles in rats. Animals were grouped into: sedentary (S); S with AAS (AAS); trained (T); and T with AAS (AAST). Exercised groups performed jumps …

Acute Effects Of A Short-Term And High Intensity Exercise On Serum Cytokines Concentrations Of Sedentary Women Submitted To A Circuit Resistance Training, Cristiane Nicioli, João Elias Nunes, Gabriela Bombarda, Fabiano Candido Ferreira

International Journal of Exercise Science: Conference Proceedings

The aim of this study was to evaluate the acute effects of short-term and high intensity exercise on serum cytokines concentrations, oxygen consumption and power of sedentary women pre and post 10 weeks of circuit resistance training (CRT). 14 women, 40.23 ± 3.9 years old, 164 ± 6.6 cm and 57.84 ± 7.7 kg were evaluated. Ventilatory threshold (WVT) and VO2peak (IVO2peak) were determined by ergoespirometric incremental test in a cycloergometer. In pre and post CRT, the women performed a test of cycling exercise with two stages: pedaling 30 minutes on WVT and 5 minutes on IVO2peak. The VO2peak was …

Proceedings Of The I International Meeting In Exercise Physiology, Sérgio Eduardo De Andrade Perez

International Journal of Exercise Science: Conference Proceedings

This meeting is the seventh in Exercise Physiology and the first International Workshop in Exercise Physiology of UFSCar, sponsored by the Exercise Physiology Laboratory, Department of Physiological Sciences – DCF, which is affiliated to the Post-Graduation Program in Physiological Sciences (PPGCFS/UFSCar). The event is designed to bring Brazilian scientists, especially from São Paulo state that work with Exercise Physiology and Health. The main objective is to put together these students and scientists interested in developing joint researches in this area. The general theme is Exercise Physiology, including athletic performance, nutrition and metabolism. Therefore, the central roundtable programmed in this year …

Overwintering Energetics Of Lepidoptera: The Effects Of Winter Warming And Thermal Variability., Caroline M. Williams

Caroline M Williams

Winter temperatures are changing rapidly, and although winter warming reduces cold stress for overwintering ectotherms, temperature-mediated increases in metabolic rate can decrease fitness in dormant insects by increasing consumption of energy reserves. Increases in thermal variability also increase energetic demands, due to non-linear thermal response curves. My objective was to quantify the negative effects of winter warming and increases in thermal variability on a range of Lepidopteran species. As overwintering insects rely on lipid catabolism, accurate lipid measurement is central to my dissertation; so I first compared four methods of lipid quantification; concluding thin layer chromatography was the only method …

Roles Of The Ion Channel Nalcn In Neuronal Excitability Control, Boxun Lu

Boxun Lu

The resting membrane potential (RMP) of a neuron is set by a complex balance between charged ions, ion channels and transporters. Many of the ion channels have been identified at the molecular level. Missing from the molecular identification has been the voltage-insensitive background sodium ‘‘leak’’ conductance that depolarizes the RMP from the equilibrium potential of potassium and provides a crucial contribution to neuronal excitability One candidate for the molecular identity of this conductance is the protein NALCN. NALCN is a previously uncharacterized orphan member in the sodium/calcium channel family. It is widely expressed in the nervous system. My thesis project …

The Proteomic Response Of Sea Squirts (Genus Ciona Congeners) To Hyposalinity Stress, James S. Koman

Master's Theses

The ascidian species Ciona savignyi and C. intestinalis are invasive species but show interspecific differences in their population response to hypo-saline stress associated with heavy winter-run off events that are predicted to become more frequent due to climate change. Despite an almost world-wide distribution, C. intestinalis seems to be more susceptible to hypo-saline stress than the geographically more limited C. savignyi. Given that the genomes of both species are fully sequenced, we were able to compare their proteomic response to both acute and chronic salinity to characterize the mechanisms that are responsible for setting tolerance limits to hyposaline conditions …

Ipla2Β Overexpression In Smooth Muscle Exacerbates Angiotensin Ii-Induced Hypertension And Vascular Remodeling, Lindsay E Calderon, Shu Liu, Wen Su, Zhongwen Xie, Zhenheng Guo, Wanda Eberhard, Ming C. Gong

Physiology Faculty Publications

OBJECTIVES: Calcium independent group VIA phospholipase A₂ (iPLA₂β) is up-regulated in vascular smooth muscle cells in some diseases, but whether the up-regulated iPLA₂β affects vascular morphology and blood pressure is unknown. The current study addresses this question by evaluating the basal- and angiotensin II infusion-induced vascular remodeling and hypertension in smooth muscle specific iPLA₂β transgenic (iPLA₂β-Tg) mice.

METHOD AND RESULTS: Blood pressure was monitored by radiotelemetry and vascular remodeling was assessed by morphologic analysis. We found that the angiotensin II-induced increase in diastolic pressure was significantly higher in iPLA₂β-Tg …

Introduction To Nanoscopy Nano-Talk, George Mcnamara

George McNamara

T7-1 is the designation for the LMRG Nanoscopy session at ABRF in Orlando, FL, on March 20, 2012. The PDF file here is a draft of my presentation.

May not be very helpful since (1) would probably help to know what is in my head and each slide will [hopefully] prompt me to say, and (2) 10 minute talk so I am going to push the "next slide" button after saying very little.

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Publisher statement:

The T7-1 Introduction to Nanoscopy Nano Talk is copyrighted (c) George McNamara, 2012. Except for (1) screenshots from research articles (which are copyrighted by …

Pubspectra - Open Data Access Fluorescence Spectra, George Mcnamara

George McNamara

The Internet is enabling greater access to spectral imaging publications, spectral graphs, and data than that was available a generation ago. The spectral imaging systems discussed in this issue of Cytometry work because reagent and hardware spectra are reproducible, reusable, and provide input to spectral unmixing and spectral components recognition algorithms. These spectra need to be readily available in order to determine what to purchase, how to use it, and what the output means. We refer to several commercially sponsored and academic spectral web sites and discuss our spectral graphing and data sites. Sites include fluorescent dye graph servers from …

Mcnamara 2011 Feature Extraction (Image Analysis), George Mcnamara

George McNamara

Feature Extraction presentation and movies in a ZIP file from a presentation I gave at ISAC 2011 in Baltomore, Md.

Feature extraction is one phrase for image analysis.

Spatial Distribution And Modulation Of Nitric Oxide Synthase In A Hypertensive Rat Model, Andrew Yannaccone

Theses and Dissertations

There are gaps in the fundamental understanding of the expression of nitric oxide synthases (NOS) in the microvasculature. We examined co-localization of NOS1 (nNOS), NOS2 (iNOS) and NOS3 (eNOS) in the spinotrapezius muscle of young adult male Wistar-Kyoto (WKY) and Spontaneously Hypertensive (SHR) rats according to fiber type using immunohistochemistry and brightfield microscopy. Data regarding fiber distribution, population and morphology data were collected. Alkaline phosphatase staining was used to determine capillary density and average number of capillaries around a fiber. Gel electrophoresis and Western blot techniques were used to compare myosin heavy chain (MHC) protein expression with fiber type population …