Physiology Commons^™

Tutorial For Using Sliceomatic To Calculate Thigh Area And Composition From Computed Tomography Images From Older Adults, Richard A. Dennis, Douglas E. Long, Reid D. Landes, Kalpana P. Padala, Prasad R. Padala, Kimberly K. Garner, James N. Wise, Charlotte A. Peterson, Dennis H. Sullivan

Center for Muscle Biology Faculty Publications

Objective

Area of muscle, fat, and bone is often measured in thigh CT scans when tissue composition is a key outcome. SliceOmatic software is commonly referenced for such analysis but published methods may be insufficient for new users. Thus, a quick start guide to calculating thigh composition using SliceOmatic has been developed.

Methods

CT images of the thigh were collected from older (69 ± 4 yrs, N = 24) adults before and after 12-weeks of resistance training. SliceOmatic was used to segment images into seven density regions encompassing fat, muscle, and bone from -190 to +2000 Hounsfield Units [HU]. The …

Effects Of Changing The Focus Of Attention On Accuracy, Acceleration, And Electromyography In Dart Throwing, Dakota R. Hitchcock, David E. Sherwood

International Journal of Exercise Science

International Journal of Exercise Science 11(1): 1120-1135, 2018. Research over the past 15 years or so has shown that an external focus on the effects of one’s movements improves performance relative to an internal focus of attention on bodily actions. More recent research has attempted to discover how the focus of attention (FOA) influences underlying motor control processes by using kinematic and EMG measures. Research has shown that an external FOA reduces EMG activity and the co-contraction between agonist and antagonist muscle groups relative to an internal FOA. The primary goal of the current study was to determine how the …

The Predictability Of Peak Oxygen Consumption Using Submaximal Ratings Of Perceived Exertion In Adolescents, Danilo V. Tolusso, Ward C. Dobbs, Michael Esco

International Journal of Exercise Science

International Journal of Exercise Science 11(4): 1173-1183, 2018. Rating of perceived exertion (RPE) extrapolation involves mathematically extending the submaximal relationship between RPE and oxygen consumption (VO₂) to maximal intensity. This technique allows practitioners to forego, potentially dangerous, maximal exertion testing while attaining accurate measures of maximal oxygen consumption used for exercise prescription. This method has been proven accurate in adults, but much less in known when applied to an adolescent population. The purpose of this study was to assess the accuracy of the RPE extrapolation as method for estimating VO_2max in adolescents. Twenty-two healthy, asymptomatic adolescents performed …

Characterizing The Aerobic And Anaerobic Energy Costs Of Polynesian Dances, Wei Zhu, David E. Lankford, Joel Reece, Daniel P. Heil

International Journal of Exercise Science

International Journal of Exercise Science 11(4): 1156-1172, 2018. This study characterized both aerobic and anaerobic energy expenditure (EE) for several Polynesian dances in a group of experienced professional Polynesian dancers. Thirteen men and 17 women were tested using indirect calorimetry to assess aerobic EE (and converted to METs), and fingertip blood lactate to estimate anaerobic EE, during both resting and dancing activities. Total EE was then computed as the sum of both aerobic and anaerobic activity energy expenditure (AEE, or EE above resting). One sample t-tests compared mean MET values for each type of dance to the 3-MET and 6-MET …

Foot-To-Foot Bioelectrical Impedance, Air Displacement Plethysmography, And Dual Energy X-Ray Absorptiometry In Resistance-Trained Men And Women, Justin Merrigan, Sina Gallo Phd, Jennifer Fields, Margaret T. Jones Phd

International Journal of Exercise Science

International Journal of Exercise Science 11(4): 1145-1155, 2018. Accurate assessment of body composition is important to athletic performance goal setting and nutritional program design. Dual-energy x-ray absorptiometry (DXA) is considered the “gold standard” in body composition assessment, yet the cost renders DXA unfeasible for many. Therefore, it is of interest to compare other body composition assessment methods to DXA in resistance-trained individuals whose focus is athletic performance. The purpose of the current study was to determine the agreement in estimates of body composition ((body fat (BF%); fat mass (FM); fat free mass (FFM)) by bioelectrical impedance analysis (BIA; Tanita SC-331S) …

The Influence Of Tissue Plasminogen Activator I/D Polymorphism On The Tpa Response To Exercise, Adam M. Coughlin

International Journal of Exercise Science

International Journal of Exercise Science 11(3): 1136-1144, 2018. The purpose was to determine if the Alu-insertion (I)/deletion (D) polymorphism of the tissue plasminogen activator (tPA) gene influences the tPA response to maximal exercise. Fifty male subjects (age = 23.6 ± 4.7 yrs) completed a maximal treadmill exercise test. Blood samples were drawn before and immediately after exercise for determination of plasma tPA antigen and activity. Isolated DNA was amplified via polymerase chain reaction, electrophoresed, and visually amplified to determine tPA genotype. Subjects were classified as possessing the D allele (D) (n = 28) or being homozygous for the I allele …

From The Editors: A Guide For Peer Review In The Field Of Exercise Science, Whitley J. Stone, James W. Navalta, Scott Lyons, Mark A. Schafer

International Journal of Exercise Science

International Journal of Exercise Science 11(1): 1112-1119, 2018. Since its inception, the mission of the International Journal of Exercise Science (IJES) has been to engage student researchers, to provide an outlet for peer-review and possible publication of their work, and to grant an opportunity for them to gain experience as peer-reviewers. The Editors of IJES take pride in providing these opportunities for student involvement, and we are constantly seeking new and innovative ways to enhance students’ professional development. As our readership has expanded across the globe and our scope has broadened to cross many Kinesiology related disciplines, we believe it …

Editorial: Ion Channel Trafficking And Cardiac Arrhythmias, Marcel A. G. Van Der Heyden, Brian P. Delisle, Hugues Abriel

Physiology Faculty Publications

No abstract provided.

Effect Of Larval Starvation On Lipid Content Of Drosophila Melanogaster Over 15 Days, Fabian Leija, Allen Gibbs

LSAMP Poster Presentations

Starvation-resistant Drosophila melanogaster, common fruit flies, deriving from 121 generations of starvation selection, have resulted in genetically, behaviorally, and physiologically different individuals compared to non-resistant counterparts. Starvation-resistant Drosophila are more obese, containing twice as much lipids as control flies.This is a result of starvation-resistant fly larva feeding for 5 days and then entering the pupa stage while non-resistant flies only feed for 4 days.

In this experiment, we hope to answer the question of whether the starvation-resistant flies are genetically predisposed to be substantially more obese than wild populations or if it is a result of this alternate behavior …

Reproductive Timing Of The Scarlet Shiner (Lythrurus Fasciolaris) In Northern Alabama, Bruce Stallsmith, Toacca Taylor, Chelsie Smith

Southeastern Fishes Council Proceedings

The Scarlet Shiner (Lythrurus fasciolaris) is a cyprinid species widely distributed in parts of the Ohio, Cumberland and Tennessee river drainages of the United States. The objective of this study was to determine the Scarlet Shiner’s reproductive schedule. Maturation of ovaries and oocytes was determined through the categorization of developmental stages from early maturing to ripe, along with the calculation of monthly gonadosomatic index (GSI) measurements over two reproductive seasons in 2012 and 2015. In both years reproductive competence began in April. Average monthly GSI for females peaked in May, followed by a slow decline through August. Average clutch …

Glutathione-Mediated Oxidative Response In Uvb-Irradiated Hacat Cells, Patrick Badger, Dr. Jason Hansen

Journal of Undergraduate Research

Ultraviolet light (UV) plays a critical role in the etiology of skin cancer, contributing to over 95% of both melanoma and non-melanoma diagnoses. It consists of several wavelengths, among which UVB is the most harmful, causing inflammation associated with sunburn and constituting the principal cause of skin cancer-related death.

An Efficient Method For Generating Conditional Cell Lines For Cellular And Molecular Research, Mark Roth, Dr. Jonathan Alder

Journal of Undergraduate Research

Conditional cell lines are widely used throughout biochemical research. The basic concept is that you can delete a gene you are studying upon the addition of a drug, to cells in culture, and then use the knockout cells to study the genes effects. This process has been facilitated by the advent of the CRISPR/Cas9 system that allows a broad range of genome editing in living cells. Cas9 introduces a double strand break in DNA at a site specified by the CRISPR (guide RNA), and by changing the CRISPR sequence researchers can introduce these double strand breaks almost wherever they want. …

B-Hydroxybutyrate Favorably Alters Muscle Cell Survival And Mitochondrial Bioenergetics, Brian Parker, Benjamin Bikman

Journal of Undergraduate Research

The growing worldwide incidence of obesity and its associated pathologies, like type 2 diabetes, has received much deserved attention. However, despite this attention and substantial research efforts, little meaningful progress has been made in slowing or reversing the growing cost and trends of obesity worldwide. As of 2008, almost 10% of medical costs in the United States were incurred due to obesity or other obesity-related diseases,ⁱ with the substantial amount of this cost stemming from the dramatically increased risk of diabetes with excess fat mass.ⁱⁱ According to the US Centers for Disease Control and Prevention, more than one …

Improving Health Through Diet And Exercise In Children, Elaine Rush, Tara Coppinger

Publications

We know that nutritious foods and quality physical activity are two of the fundamental needs for normal growth and development of children. Food based dietary guidelines and physical activity recommendations have universal evidence base but the teaching of fundamental movement skills is limited without support. Given the importance of prevention of childhood obesity the distal drivers also need to be examined including poverty. Interventions trialled in carefully controlled conditions while shown to have efficacy are not effective in the real world. There is a need for more research and rigorous evaluation of interventions in combinations of settings and with innovative …

Lipopolysaccharide‐Induced Inflammation Does Not Alter Muscle Spindle Afferent Mechanosensation Or Sensory Integration In The Spinal Cord Of Adult Mice, Dasha Zaytseva, Anusha Allawala, Joy Franco, Shea Putnam, Adam Abtahie, Nina Bubalo, Connor Criddle, Tuan Nguyen, Peter Nguyen, Shreejit Padmanabhan, Puneet Sanghera, Martina Bremer, Tzvia Abramson, Katherine A. Wilkinson

Faculty Publications, Biological Sciences

Inflammation is known to alter nervous system function, but its effect on muscle spindle afferent mechanosensation and sensory integration in the spinal cord has not been well studied. We tested the hypothesis that systemic inflammation induced by an intraperitoneal injection of the endotoxin lipopolysaccharide (LPS; 7.5 × 105 endotoxin units/kg 18 h before experiment) would alter muscle spindle afferent mechanosensation and spinal cord excitability to Group Ia input in male and female adult C57Bl/6 mice. LPS injection caused a systemic immune response, evidenced by decreased white blood cell, monocyte, and lymphocyte concentrations in the blood, increased blood granulocyte concentration, and …

Voltage Effects On Muscarinic Acetylcholine Receptor-Mediated Contractions Of Airway Smooth Muscle, Iurii Semenov, Robert Brenner

Bioelectrics Publications

Studies have shown that the activity of muscarinic receptors and their affinity to agonists are sensitive to membrane potential. It was reported that in airway smooth muscle (ASM) depolarization evoked by high K⁺ solution increases contractility through direct effects on M3 muscarinic receptors. In this study, we assessed the physiological relevance of voltage sensitivity of muscarinic receptors on ASM contractility. Our findings reveal that depolarization by high K⁺ solution induces contraction in intact mouse trachea predominantly through activation of acetylcholine release from embedded nerves, and to a lesser extent by direct effects on M3 receptors. We therefore devised …

End It Now, Melissa Ponce-Rodas

Lake Union Herald

No abstract provided.

Effect Of Exercise Volume On Hdl-Cholesterol: A 7-Year Case Study, David P. Swain

Human Movement Studies & Special Education Faculty Publications

Introduction: While exercise is known to increase HDL-cholesterol, it is not known if larger exercise volumes produce larger increases in HDL. However, the range of exercise volumes used in most training studies is limited. This report presents a case study of a middle-aged male who engaged in large variations of exercise volume over 7 years while frequently measuring HDL. The purpose was to determine if large increases in exercise volume were associated with larger increases in HDL than previously reported.

Methods: The subject maintained detailed logs of his main form of exercise, bicycling. These logs were analyzed to determine the …

Technology-Based Physical Activity Self-Monitoring Among College Students, Zack Papalia, Oliver Wilson, Melissa Bopp, Michele Duffey

International Journal of Exercise Science

International Journal of Exercise Science 11(7): 1096-1104, 2018. Understanding the relationship between college students’ physical activity (PA) self-monitoring and PA levels has the potential to inform initiatives to promote PA. This study’s purpose was to examine the prevalence of technology-based self-monitoring among college students the potential relationship between device usage, goal setting behaviors, PA enjoyment, and PA levels. An online survey assessed students' demographics, current PA level, technology-based PA self-monitoring, and psychosocial outcomes. Independent t-tests examined differences in PA level and psychosocial outcomes by device use. 55.5% of the final sample (N=1,154) reported technology-based self-monitoring. Mobile phone app-based PA tracking …

Can Heart Rate Variability Predict The Second Metabolic Threshold In Young Soccer Players?, Gustavo S. Ribeiro, Ubiratan R. Pereira, André L. Lopes

International Journal of Exercise Science

International Journal of Exercise Science 11(2): 1105-1111, 2018. Heart rate variability (HRV) is an effective method to assess the influence of the autonomic nervous system, which may be directly linked to metabolic demand. The aim of the study was to determine if the second metabolic threshold can be identified by HRV. Thirteen athletes were assessed in cardiopulmonary exercise test with concomitant gas analysis. The RR intervals (RRi) were plotted in a spreadsheet for graphics analysis and the point at which there was a shift in the RRi curve was determined as RRiT₂. The second ventilatory threshold (VT_{2 …}