Characterizing The Role Of Pa5189 Of Pseudomonas Aeruginosa In Deletion And Overexpression Mutants, 2024 University of Nebraska at Omaha
Characterizing The Role Of Pa5189 Of Pseudomonas Aeruginosa In Deletion And Overexpression Mutants, Seh Na Mellick
Theses/Capstones/Creative Projects
In the context of rising multidrug resistance in biofilm-forming pathogens like Pseudomonas aeruginosa, this study investigates the role of the understudied transcription factor PA5189 in antibiotic resistance and biofilm formation. PA5189 deletion and overexpression mutants were created in a parent P. aeruginosa strain using pEX18Tc-based recombinant suicide vectors, with genotypic verification of putative triparental conjugants achieved through restriction digestion and PCR. The study revealed that PA5189 overexpression significantly increases resistance to commonly used broad spectrum antibiotics such as ciprofloxacin and imipenem. Additionally, differential expression of PA5189 was found to notably affect biofilm formation, with variations contingent on the nutrient …
Materials And Methods Developed For The Recording And Analysis Of Behavior In The Common Marmoset (Callithrix Jacchus)., 2024 University of Nebraska at Omaha
Materials And Methods Developed For The Recording And Analysis Of Behavior In The Common Marmoset (Callithrix Jacchus)., Christian Wintle
UNO Student Research and Creative Activity Fair
Materials and methods developed for the recording and analysis of behavior in the common marmoset (Callithrix jacchus).
Christian J. Wintlea, Jordan B. Hernandeza,b,c, Dobromir Dotovd, and Jonathan B. Claytona,b,e,f,g
aDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA
bNebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA
cDepartment of Genetics, Cell Biology, and Anatomy, University of Nebraska Medical Center, NE, USA
dDepartment of Biomechanics, University of Nebraska at Omaha, Omaha, NE, USA
eDepartment of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, …
Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, 2024 California State Polytechnic University, Humboldt
Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund
IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt
Out of the nearly one trillion species of microbiota estimated to inhabit Earth only ten thousand have been cultured in the laboratory. Culturing continues to play a vital role in determining the physiology and ecologic function of individual bacteria in microbial communities and for microbes associated with host organisms one of the major challenges is developing microbiological media that mimics the bacteria’s natural environment enough to promote growth. Here, we target bacteria associated with the estuary anemones Diadumene lineata and Metridium senile by developing a novel medium that uses anemone tissue as the sole source of nutrients. We further measured …
Investigating The Mechanisms Of Surface Sensing Using Motility Appendages By Pseudomonas Aeruginosa Pa14, 2024 Dartmouth College
Investigating The Mechanisms Of Surface Sensing Using Motility Appendages By Pseudomonas Aeruginosa Pa14, Christopher James Geiger
Dartmouth College Ph.D Dissertations
Biofilms are surfaced attached communities of cells encased in an extracellular matrix. The transition from free-swimming planktonic cells to a surface attached biofilm begins with cellular changes that occur after surface contact. This process is known as "surface sensing" and the opportunistic pathogen Pseudomonas aeruginosa PA14 uses its two motility appendages, type IV pili (T4P) and a single, polar flagellum to sense and traverse surfaces. The first cellular changes to occur within this organism upon surface contact is an increase in the second messengers cAMP and cdi- GMP. While the genes involved in surface sensing by P. aeruginosa are known, …
Knowledge, Attitude, And Practice Regarding Deep Dentinal Caries Removal Before Root Canal Treatment And Restoration Among Practicing Dental Surgeons, 2024 Department of Prosthodontics and Crown & Bridge, Institute of Dental Sciences, Siksha ‘O’ Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, India
Knowledge, Attitude, And Practice Regarding Deep Dentinal Caries Removal Before Root Canal Treatment And Restoration Among Practicing Dental Surgeons, Sangram Panda, Anamika Sinha, Shakti Rath, Kajal Kiran Sahoo
Palestinian Medical and Pharmaceutical Journal
This article aims to determine the knowledge, attitude, and practice regarding Deep Dentinal Caries Removal before Root Canal Treatment (RCT) and Restoration among practicing dental surgeons. A descriptive cross-sectional study was executed amongst dental surgeons practicing with undergraduate and post-graduate degrees. A Google questionnaire form was developed and distributed, and 111 authentic entries were short-listed. Data obtained from the study were recorded in Microsoft Excel 2007 version, and SPSS version 18.0 was used for statistical analysis. Most dental surgeons who participated in the study practiced for over 15-20 years. Among them, 60% believe in removing dentinal caries before RCT; 83.8% …
Antioxidant Properties Of Kombucha Beverage Infused With Ganoderma Lucidum And Green Tea From Camellia Sinensis (L.) Kuntze With Several Fermentation Times, 2024 Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN). Cibinong, 16911, West Java, Indonesia
Antioxidant Properties Of Kombucha Beverage Infused With Ganoderma Lucidum And Green Tea From Camellia Sinensis (L.) Kuntze With Several Fermentation Times, Rizki Rabeca Elfirta, Pamungkas Rizki Ferdian, Iwan Saskiawan, Tri Hadi Handayani, Kayla Faza Gustafri Mandalika, Rini Riffiani, Kasirah Kasirah, Ukhradiya Magharaniq Safira Purwanto
Karbala International Journal of Modern Science
Kombucha is a functional beverage produced through the fermentation of infused tea or Camelia sinensis (L.) Kuntze (CS) by a symbiotic culture of bacteria and yeast. Apparently, the substrate of kombucha can be substituted to enhance its functional properties. Ganoderma lucidum (GL) is a potential substrate reported to have health benefits. This study aims to evaluate antioxidant properties by comparing different formulations and fermentation times. The formulations prepared in the present study varied in the compositions of GL and CS substrates, namely F1 (100% GL), F2 (75% GL: 25% CS), F3 (50% GL: 50% CS), F4 (25% GL: 75% CS), …
Investigating Optimal Laboratory Growth Conditions Of Gracilibacillus Halotolerans In Media Supplemented With Salt, 2024 Roseman University of Health Sciences
Investigating Optimal Laboratory Growth Conditions Of Gracilibacillus Halotolerans In Media Supplemented With Salt, Isaac Young
Annual Research Symposium
As interest continues to grow in the field of persister cells and their morphology, there arises an ever-evolving desire to further understand specific strains of bacteria that exemplify the qualities of seemingly anomalous survival regardless of anti-bacterial treatment. In the case of the Gracilibacillus halotolerans, a halotolerant extremophile extracted from the Great Salt Lake with known persistent characteristics, uncovering its optimal growth conditions was essential for future investigations. Identifying the optimal salinity for the growth of G. halotolerans will allow us to standardize our growth methods, uncover several mechanisms of saline tolerance, and add to future investigations of persistence with …
Interplay Of Periodontal Bacterial Metabolites In The Progression Of Coronary Artery Disease: A System Biological Approach, 2024 Roseman University of Health Sciences
Interplay Of Periodontal Bacterial Metabolites In The Progression Of Coronary Artery Disease: A System Biological Approach, Janakiraman V
Annual Research Symposium
Purpose:
The purpose of this study is to investigate the intricate relationship between periodontal disease (PD) and coronary artery disease (CAD), as evidenced by epidemiological associations. Metalloproteinase inhibitor (TIMP1) plays a pivotal role in cellular signaling, differentiation, cell death, and migration by binding to target metalloproteinases, forming complexes with other molecules (collagenases) to inactivate them. However, the expression of TIMP1 is reduced in both PD and CAD, leading to an upregulation of other metalloproteinases. This research explores the hypothesis that metabolites released from (Porphyromonas gingivalis), a prevalent bacterium in atherosclerotic patients, may inhibit TIMP1, thereby influencing CAD progression. …
A Rationally Designed Antimicrobial Peptide From Structural And Functional Insights Of Clostridioides Difficile Translation Initiation Factor 1, 2024 The University of Texas Rio Grande Valley
A Rationally Designed Antimicrobial Peptide From Structural And Functional Insights Of Clostridioides Difficile Translation Initiation Factor 1, Elvira Alanis, Faith Aguilar, Niaz Banaei, Frank B. Dean, Alexa Villarreal, Miguel Alanis, Karen Lozano, James M. Bullard, Yonghong Zhang
School of Integrative Biological and Chemical Sciences Faculty Publications and Presentations
ABSTRACT: A significant increase of hospital-acquired bacterial infections during the COVID-19 pandemic has become an urgent medical problem. Clostridioides difficile is an urgent antibiotic-resistant bacterial pathogen and a leading causative agent of nosocomial infections. The increasing recurrence of C. difficile infection and antibiotic resistance in C. difficile has led to an unmet need for the discovery of new compounds distinctly different from present antimicrobials, while antimicrobial peptides as promising alternatives to conventional antibiotics have attracted growing interest recently. Protein synthesis is an essential metabolic process in all bacteria and a validated antibiotic target. Initiation factor 1 from C. difficile (Cd-IF1) …
College Of Natural Sciences 2023 Year-End Publication, 2024 South Dakota State University
College Of Natural Sciences 2023 Year-End Publication, College Of Natural Sciences
College of Natural Sciences Newsletters and Reports
Page 1 Dean's Message
Page 3 Department Highlights
Page 4 One Day for State
Page 5 Noble Prize Winner Speaks on Campus
Page 6-7 Faculty Excellence
Page 8-9 Student Excellence
Page 10 Outreach Program
Page 10 Events and Traditions
Page 11 Connections Abroad
Page 12 Student Spotlight
Page 13 Alumni Spotlight
Page 14 First Ever Drone Day
Page 15 Grand Opening of POET Bioproducts Center
Page 16 Work Anniversaries
Finding Gene Candidates That Interact With Mara To Control Hila Expression In Salmonella Enterica, 2024 University of Missouri, St. Louis
Finding Gene Candidates That Interact With Mara To Control Hila Expression In Salmonella Enterica, Kylee Hempel
Undergraduate Research Symposium
Salmonella is a well-known pathogen that is linked to food born illnesses common around the world. This pathogen can cause symptoms such as fever, diarrhea, and chills in the host. One of the mechanisms Salmonella uses to infect its host’s epithelial cells deals with the S. typhimurium pathogenicity island 1 (SPI-1). Expression of SPI-1 is activated in response to environmental signals that correlate with the ileum of the small intestine (low oxygen, high osmolarity, and neutral pH). HilA is the central activator of the SPI-1 complex, and when the transcription factor, MarA, is over-expressed in Salmonella, hilA transcription is …
A Survey Of Fusarium Oxysporum In Georgia Hemp Farms And Vegetable Gardens, 2024 Georgia College and State University
A Survey Of Fusarium Oxysporum In Georgia Hemp Farms And Vegetable Gardens, Wesley Demontigny, Christopher D. Burt, Jaiden Stidston
Georgia Journal of Science
Recent changes in U.S. federal law have recognized the genetic and chemical differences between hemp and marijuana (Cannabis sativa L.), resulting in the legalization of hemp in the United States. The barriers to this plant’s growth in the state of Georgia are not well understood due to its prohibition for the past 80 years. Among the many possible obstacles is fungal disease. Fusarium oxysporum (Schlecht. emend. Snyder & Hansen) is a fungus that damages various crops and was previously researched as a biocontrol agent for C. sativa. Thus, this study surveyed hemp farms in Georgia to determine the prevalence …
Effectiveness Of Mycorrhizae And Vermicompost Seed Inoculation For Germination, Vegetative Growth, Cannabinoid Content, And Cured Flower Weight Of Cbd-Rich Hemp (Cannabis Sativa L.), 2024 Texas State University
Effectiveness Of Mycorrhizae And Vermicompost Seed Inoculation For Germination, Vegetative Growth, Cannabinoid Content, And Cured Flower Weight Of Cbd-Rich Hemp (Cannabis Sativa L.), Hannah Boyer, Nicole Wagner, Merritt Drewery
Journal of Agricultural Hemp Research
Effective germination and vigorous growth of hemp cultivars is paramount to cultivators’ ability to produce high-quality hemp products. Beneficial bacteria and fungi are known symbionts to plants and are used in regenerative agriculture to increase plant health and crop yield. This pilot study investigated the effect of microbial seed inoculation on germination rate, plant height, cured flower weight, and cannabinoid content of cannabidiol (CBD)-rich hemp (Cannabis sativa L). The experiment included a control and the following treatments: 1) seed inoculation of Great White â Premium Mycorrhizae (GW), 2) seed inoculation of vermicompost (V), and 3) seed inoculation of Great …
The Ecological Responses To Hydroperiod Of Wetland Plant Species Determined By Manipulated Soil Surface Elevation (Marsh Organs), 2024 Louisiana State University
The Ecological Responses To Hydroperiod Of Wetland Plant Species Determined By Manipulated Soil Surface Elevation (Marsh Organs), Brandon Wolff
LSU Master's Theses
As sea level rise, subsidence, and abandonment of natural deltaic processes due to a highly engineered Mississippi River continue to threaten Louisiana’s coastal wetlands, the need for a system-wide understanding of natural wetland land-building and preservation processes has never been greater. A key component of any wetland is the ever-changing water environment that periodically floods and dries the marsh platforms. The flooding depth, duration, and frequency, known as the hydroperiod, along with salinity and soil fertility are key determining factors of vegetation and marsh types at a particular location. Different types of vegetation will have different growth characteristics such as …
Secreted Igm Modulates Il-10 Expression In B Cells, 2024 Thomas Jefferson University
Secreted Igm Modulates Il-10 Expression In B Cells, Shannon Mcgettigan, Lazaro Aira, Gaurav Kumar, Romain Ballet, Eugene Butcher, Nicole Baumgarth, Gudrun Debes
Department of Microbiology and Immunology Faculty Papers
IL-10+ B cells are critical for immune homeostasis and restraining immune responses in infection, cancer, and inflammation; however, the signals that govern IL-10+ B cell differentiation are ill-defined. Here we find that IL-10+ B cells expand in mice lacking secreted IgM ((s)IgM–/–) up to 10-fold relative to wildtype (WT) among all major B cell and regulatory B cell subsets. The IL-10+ B cell increase is polyclonal and presents within 24 hours of birth. In WT mice, sIgM is produced prenatally and limits the expansion of IL-10+ B cells. Lack of the high affinity …
The Inaugural Mbio Junior Editorial Board—Lessons Learned And The Path Forward Toward Improving The Peer Review Process, 2024 University of Cape Coast
The Inaugural Mbio Junior Editorial Board—Lessons Learned And The Path Forward Toward Improving The Peer Review Process, Cynthia Ayefoumi Adinortey, Stephen K. Dolan, Sarah Doore, Rebeccah Lijek, Diana Priscila Pires, Wenqi Yu, Elizabeth B. Draganova, Lennart Schada Von Borzyskowski
Molecular Biosciences Faculty Publications
The inaugural Junior Editorial Board (JEB) of mBio consisted of 64 early-career researchers active from 2022 to 2023. The goal of the JEB was to train early-career researchers in the art of peer review under the guidance of experienced editors. JEB members gained hands-on experience in peer review by participating in modules detailing the publishing process through the lenses of the journal, editor, and reviewer. Ultimately, JEB members applied this new knowledge by reviewing mBio manuscripts. Here, we summarize the background, the mission, and the achievements of the first mBio JEB. We also include possible trajectories for the future editions …
Why Should Early-Career Scientists Publish In Society Journals, 2024 Clemson University
Why Should Early-Career Scientists Publish In Society Journals, Stephen K. Dolan, Lori D. Banks, Wenqi Yu
Molecular Biosciences Faculty Publications
In this editorial, written by early-career scientists, we advocate for the invaluable role of society journals in our scientific community. By choosing to support these journals as authors, peer reviewers, and as editors, we can reinforce our academic growth and benefit from their re-investment back into the scientific ecosystem. Considering the numerous clear merits of this system for future generations of microbiologists and more broadly, society, we argue that early-career researchers should publish our high-quality research in society journals to shape the future of science and scientific publishing landscape.
Gut Dysbiosis Correlates With Covid-19 Severity, 2024 Claremont Colleges
Gut Dysbiosis Correlates With Covid-19 Severity, Ednita Escobar
CMC Senior Theses
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) impacts not only respiratory but also gut and oral microbiomes, influencing the manifestations of COVID-19. Each person’s unique microbiome consists of microorganisms such as bacteria, fungi, and viruses, some of which are symbiotic and others potentially harmful. Alterations in the gut microbiome, specifically, can affect the severity and symptoms of COVID-19. A review of studies indicate a pattern of gut dysbiosis in COVID-19 patients characterized by a decrease in beneficial microbiota and/or an increase in opportunistic pathogens. This gut imbalance correlates with disease severity, suggesting that the gut microbiome plays a significant role …
A Comparison Of Adenosine Triphosphate With Other Metrics Of Microbial Biomass In A Gradient From The North Atlantic To The Chesapeake Bay, 2024 Old Dominion University
A Comparison Of Adenosine Triphosphate With Other Metrics Of Microbial Biomass In A Gradient From The North Atlantic To The Chesapeake Bay, Alexander B. Bochdansky, Amber A. Beecher, Joshua R. Calderon, Alison N. Stouffer, Nyjaee N. Washington
OES Faculty Publications
A new, simplified protocol for determining particulate adenosine triphosphate (ATP) levels allows for the assessment of microbial biomass distribution in aquatic systems at a high temporal and spatial resolution. A comparison of ATP data with related variables, such as particulate carbon, nitrogen, chlorophyll, and turbidity in pelagic samples, yielded significant and strong correlations in a gradient from the tributaries of the Chesapeake Bay (sigma-t = 8) to the open North Atlantic (sigma-t = 29). Correlations varied between ATP and biomass depending on the microscopic method employed. Despite the much greater effort involved, biomass determined by microscopy correlated poorly with other …
Electrospun Tamarindus Indica-Loaded Antimicrobial Pmma/Cellulose Acetate/Peo Nanofibrous Scaffolds For Accelerated Wound Healing: In-Vitro And In-Vivo Assessments, 2024 The British University in Egypt
Electrospun Tamarindus Indica-Loaded Antimicrobial Pmma/Cellulose Acetate/Peo Nanofibrous Scaffolds For Accelerated Wound Healing: In-Vitro And In-Vivo Assessments, Elbadawy A. Kamoun, Elbadawy A. Kamoun
Nanotechnology Research Centre
In this work, Tamarindus indica (T. indica)-loaded crosslinked poly(methyl methacrylate) (PMMA)/cellulose acetate (CA)/poly(ethylene oxide) (PEO) electrospun nanofibers were designed and fabricated for wound healing applications. T. indica is a plant extract that possesses antidiabetic, antimicrobial, antioxidant, antimalarial and wound healing properties. T. indica leaves extract of different concentrations were blended with a tuned composition of a matrix comprised of PMMA (10 %), CA (2 %) and PEO (1.5 %), and were electrospun to form smooth, dense and continuous nanofibers as illustrated by SEM investigation. In vitro evaluation of T. indicaloaded nanofibers on normal human skin fibroblasts (HBF4) revealed a high …