Veterinary Pathology and Pathobiology Commons^™

Epidemiological Survey Of Abortion In Camels (Camelus Dromedarius) And Histopathological Investigation Of Toxoplasmosis And Neosporosis In Those Animals From Slaughterhouses In Southeastern Algeria, Amira Djeddou-Benabid, Hind Houssou, Omar Bennoune, Ferhat Nouicer, Amel Djaout

The Thai Journal of Veterinary Medicine

Toxoplasma gondii and Neospora caninum are heteroxenous apicomplexan parasites recognized as major causes of reproductive disorders in numerous mammalian species. The present study aimed to assess through an epidemiological survey, the prevalence of reproductive losses in 75 dromedary herds during the period 2022-2025, as well as the associated risk factors, and to detect these protozoa by histopathological analysis in 63 dromedaries slaughtered at the slaughterhouse of El Oued province (126 samples: 63 hearts and 63 diaphragms). The survey revealed that 80% of the herds had a history of abortions, of which 60% also reported cases of stillbirth. Risk factor analysis …

Microrna Expression In Putative Cancer Stem Cells Isolated From Canine Mammary Gland Tumour Cells With Differing Sensitivity To Doxorubicin, Kabiru Sahabi, Gayathri Thevi Selvarajah, Mohd Mokrish Md Ajat, Rasedee Abdullah, Cheah Y. Kqueen

The Thai Journal of Veterinary Medicine

Cancer stem cells (CSCs) are capable of initiating tumour development and causing recurrence after treatment. The objective of this study was to determine the miRNAs, downstream target mRNAs, and signalling pathways in CSCs isolated from doxorubicin-sensitive and doxorubicin-resistant canine mammary adenocarcinoma cell lines. MicroRNA expression was profiled using Agilent SurePrint^® microarrays. MicroRNA data were analyzed using Agilent GeneSpring^® software, and TargetScan and WikiPathways were used to identify genes and pathways targeted by the differentially expressed miRNAs. Six miRNAs were differentially expressed in CSCs isolated from doxorubicinsensitive cells, while fourteen miRNAs were differentially expressed in CSCs isolated from doxorubicin-resistant …

Surveillance Of A Novel Vesivirus Discovered In An Urban Mephitis Mephitis Population, Amanda E. Diggins

Honors Theses and Capstones

Caliciviridae is a family of non-enveloped, positive-sense, single stranded RNA viruses, including eleven defined genera that contain important pathogens of humans and animals. Within the Caliciviridae family, the genus Vesiviridae includes feline calicivirus, a leading cause of upper respiratory disease in cats, and vesicular exanthema of swine virus, a historical disease of domestic swine that has now been implicated in several marine mammal vesicular diseases, one of which has been confirmed zoonotic. Urban landscapes create a unique environment for rapid intra- and interspecies transmission of viruses. Clustered resources attract high-density populations of synanthropic wildlife species, increasing spatial overlap and heightening …

Production, Characterization, And Diagnostic Application Of Monoclonal Antibodies Against The N-Protein Of Avian Metapneumovirus Subgroup B, Ravi Gupta

Electronic Theses and Dissertations

Avian metapneumovirus (aMPV) is a respiratory pathogen that causes Turkey Rhinotracheitis (TRT) in turkeys and Swollen Head Syndrome (SHS) in chickens. The infection leads to facial swelling, nasal discharge, reduced egg production, and poor growth. This virus is classified into four subgroups: aMPV-A, B, C, and D, with two other novel subgroups, in which aMPV-A, B, and C are most prevalent in the commercial flocks. In early 2024, aMPV-A and B outbreaks have been reported in the US, resulting in severe economic losses. An economic assessment estimates around $112 million in losses in Minnesota and up to $434 million in …

Recovery And Characterization Of Moraxella Species From Bovine Specimens, Laura G. Leger

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The family Moraxellaceae is made up of a diverse group of 24 named species that fall into three phylogenetic groups. While most Moraxella spp. act as commensal respiratory and/or ocular flora of mammals, some are associated with disease. One of these diseases, infectious bovine keratoconjunctivitis (IBK), has significant welfare and economic impacts to both cattle and cattle producers. Moraxella bovis is the only experimentally determined cause of IBK, however, an association of Moraxella bovoculi with disease has been proposed. Currently, available vaccines do not have high levels of efficacy in the field. Culture based methods are the gold standard for …

Prevalence Of Cytauxzoon Felis In Ticks From South-Central Kansas And Northwest Arkansas, Nicole E. Mcilvain

Animal Science Undergraduate Honors Theses

Cytauxzoon felis is a protozoan that causes an often-fatal illness in felines known as cytauxzoonosis, or bobcat fever. Ticks are vectors of this pathogen, with Amblyomma americanum being the most common vector. Previous studies have noted a correlation between peak tick season and peak cytauxzoonosis diagnosis. For cytauxzoonosis, minimal treatments exist, and scientists have not found a cure, which is why focus needs to be on preventing disease. Knowledge on the prevalence of C. felis in different tick species helps veterinarians and pet owners be aware of the potential for their cat developing cytauxzoonosis if one or more ticks are …

Biocompatibility And Anti-Inflammatory Effects Of Thai Cannabidiol-Containing Topical Anesthetic Gel Prototype In A Dermal Irritation Test, Pasutha Thunyakitpisal, Supattarayan Thongjit, Apitchaya Pruksametanan, Wijit Banlunara Assoc. Prof.

The Thai Journal of Veterinary Medicine

The objective of this study is to investigate and compare the effect of cannabidiol (CBD)-containing and non-CBD topical anesthetic gel prototypes on skin irritation in rabbits at 24, 48, and 72 h post-application. Topical anesthetic gel prototypes containing 0.1% CBD and without CBD were developed. Six rabbits (Oryctolagus cuniculus) were used for skin irritation testing according to ISO 10993-23: 2021. The animals were randomly and equally divided into two groups (CBD and non-CBD gel groups). The gels were applied to the animals’ skin at the dorsal area of the flank with good contact for 4 h. Distilled water …

Hematologic And Serum Biochemical Reference Intervals For Formosan Sika Deer (Cervus Nippon Taiouanus) From A Semi-Free-Ranging Population In Taiwan, Chen-Wei Wang, Ke-Lun Chen, Hung-Yi Wu, Chia-Ying Lin

The Thai Journal of Veterinary Medicine

The Formosan sika deer (Cervus nippon taiouanus), an endemic Taiwanese subspecies, holds a pivotal role in local conservation. This study addresses a significant knowledge gap by establishing population-specific reference intervals (RIs) for this species, as existing RIs are based on limited captive populations and may not accurately reflect the health of semi-free-ranging cohorts. We analyzed 632 sampling events from 232 healthy adult deer within Kenting National Park over 12 years (2013–2024). This study adhered to ASVCP guidelines, establishing instrument-specific RIs and employing linear mixed-effects models (LMMs) to evaluate the complex interactions among analytical instruments, sex, and body weight. …

Epidemiological Inferences Of The Impacts Of Winter Ticks On Moose Health, Krishna S. Woerheide

Department of Entomology: Dissertations, Theses, and Student Research

The North American Moose (mooz in Anishinaabemowin, Ojibwe language, Alces alces), a culturally significant subsistence species for the Anishinaabe people, faces population decline due to calf predation, pathogen transmission, and parasitism by the winter tick (Dermacentor albipictus), all exacerbated by climate change. This study explores the impact of winter tick infestations on moose health, with a focus on natural mineral seeps as potential transmission hotspots in moose habitats within the Grand Portage Indian Reservation (Gichi Onigaming), on Isle Royale National Park (Minong), and within the 1854 Ceded Territory (greater northeastern Minnesota) - ancestral and present homelands of …

Proteomic Comparison Of Canine Gallbladder Versus Hepatic Neuroendocrine Neoplasms, Nadia Richmond

LSU Master's Theses

Hepatobiliary neuroendocrine neoplasms (HBNENs) are uncommon tumors in people and dogs, and the limited understanding of their biologic behavior often poses a diagnostic and prognostic challenge in the clinical setting. Though only a low number of cases have been described in dogs, there is evidence to indicate that primary gallbladder NENs may carry a more favorable prognosis compared to primary hepatic NENs. The emergence of liquid chromatography-mass spectrometry (LC-MS) in proteomic analysis has permitted large-scale analysis of neoplastic proteomes, allowing for better understanding of tumor pathophysiology while simultaneously enabling the rapid identification of protein biomarkers. Previous work in our laboratory …

Region-Specific Hematological And Biochemical Reference Intervals For Southern Thai Fighting Bulls, Dhiravit Chantip, Nantaporn Chooruang, Kitikarn Sakuna, Niramol Thongtem, Wiruntita Bohman

The Thai Journal of Veterinary Medicine

Accurate health assessments are vital for the performance and welfare of fighting bulls. Existing hematological and biochemical reference intervals (RIs) for cattle may not adequately reflect the unique characteristics of fighting bulls due to breed, age, and geographical differences. This study aimed to develop and apply region-specific RIs for hematological and biochemical parameters in fighting bulls from southern Thailand. A retrospective analysis of laboratory records was conducted on 1,460 healthy fighting bulls enrolled in an annual health check program at the Livestock Animal Hospital, Faculty of Veterinary Sciences, Rajamangala University of Technology Srivijaya, from January 2016 to December 2023. Blood …

Correlation Of Classical Swine Fever (Csf) Antibody Protective Level Detected By Enzyme-Linked Immunosorbent Assay And Serum Neutralization Test, Chairani Ridha Maghfira, Nguyen Thanh Che, Apisit Kittawornrat, Roongroje Thanawongnuwech, Yaowalak Panyasing

The Thai Journal of Veterinary Medicine

Protective antibody against classical swine fever virus (CSFV) is an important parameter used for disease monitoring in CSFV-endemic areas. Enzyme-linked immunosorbent assay (ELISA) is a simple and practical serological assay for CSFV antibody detection. However, the use of CSFV ELISAs in CSFV antibody-positive herds, i.e., CSFVvaccinated or -previously infected herds, was limited by an application of the test results. This study aimed to evaluate the correlation and estimation of protective antibody levels detected by ELISA and serum neutralization assay. A total of 522 negative and positive serums were tested by SN and ELISA in parallel. Comparisons of sample-to-positive (S/P) values …

Molecular Detection And Characterization Of Histomonas Meleagridis In Fighting Cocks Thailand, Piyanan Taweethavonsawat, Mangsatabam Norjit Singh, Sawang Kesdangsakonwut, Wanarit Jitsamai, Chongtham Sonia, Cherdpong Phupolpan

The Thai Journal of Veterinary Medicine

Histomoniasis caused by the protozoa Histomonas meleagridis is a disease of gallinaceous birds which is of special importance to the poultry industry. The turkey is highly susceptible and results in high mortality. However, the disease is less severe in chickens but sometimes leads to mortality especially in those reared in the free-range system. During the present study, four dead fighting cocks were presented to the Veterinary Diagnostic Laboratory, Livestock Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University for post-mortem examination. Based on history and pathological changes, it was preliminarily diagnosed as histomoniasis. The affected livers and caeca samples were collected …

A Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia In A Scottish Fold Cat: A Case Report, Rattanin Chiewcharnvalijkit, Weerapat Chawanlawuthi, Kittara Chanmongkolpanit, Tanit Kasantikul, Nlin Arya, Sirintra Sirivisoot, Sirima Chatrungruengkul, Duangtip Chatchaisak

The Thai Journal of Veterinary Medicine

A 2-year-old intact female Scottish fold cat was presented to the Prasu-Arthorn Animal Hospital, Faculty of Veterinary Science, Mahidol University, for a right nephrectomy because a renal mass was suspected. During the physical examination, the animal had an intra-abdominal mass with abdominal discomfort. No history of vomiting and diarrhea was reported. The blood chemistry and hematology revealed a markedly low albumin/globulin ratio and mild anemia with normal leukogram. The abdominal ultrasonographic examination and CT scan revealed an intestinal mass at the ileocecocolic junction. The mass was surgically excised, and jejuno-colic anastomosis was performed. Grossly, the mass was firm, very well …

Synchronous Tri-Cavitary Biphasic Malignant Mesothelioma In A Dog Presenting With Dyspnea, Nawarus Prapaiwan, Tawewan Issarankura Na Ayudhaya, Poonavit Pichayapaiboon, Onrampha Tanglakmankhong

The Thai Journal of Veterinary Medicine

Malignant mesothelioma is an uncommon tumor that has a challenging diagnosis and a tendency for late diagnosis. A 7-year-old intact male dog presented with respiratory distress. Thoracic radiography revealed pleural effusion and bone reactions at multiple ribs. A computerized tomography scan with rib biopsy was suggested as a diagnosis plan. Unfortunately, the dog died. Gross pathological findings demonstrated multifocal to coalescing, pinkish-white firm masses scattered over the parietal pleural surface, extended to the serosa of the diaphragm and the pericardium, making them adhere to each other and causing the coalescence of a white irregular mass at the diaphragm. Multiple white …

An Accidental Discovery Of Ductal Adenocarcinoma Of The Pancreas In An Adult Horse With Severe Abdominal Pain, Thita Wonghanchao, Kanokpan Sanigavatee, Chanoknun Poochipakorn, Onjira Huangsaksri, Phudit Maneesaay, Rungrueang Yodsheewan, Metha Chanda

The Thai Journal of Veterinary Medicine

A native pony, eight years old and male, was tentatively diagnosed with abdominal pain and treated immediately. Sequential hematological profiles revealed increased levels of aspartate aminotransferase (AST), alkaline phosphatase (ALP), and creatinine, as well as reduced albumin. After the pony's death, which occurred eight days after treatment began, a necropsy was performed. The liver's external surface showed small foci, but no gross lesion was observed in the pancreas, which was enlarged. Haematoxylin and eosin (H&E) staining of the liver showed multifocal inflammation in the hepatic parenchyma, coinciding with bile duct hyperplasia. The pancreas H&E staining showed well-differentiated tubular glands with …

Quantitative Evaluation Of Different Fixatives For Masson Trichrome Staining In Canine Intestinal Tissue: An Image Analysis-Based Comparative Study, Siriluck Juntausa, Kongkiat Srisuwatanasagul, Kittipot Kongsonthana, Punnarat Vibulchan, Sayamon Srisuwatanasagul

The Thai Journal of Veterinary Medicine

Neutral buffer formalin (NBF) has been widely used as a fixative for histological staining for ages. However, for some special staining, such as Masson trichrome staining, it has been proposed that the suitable fixative was Bouin’s solution. The purpose of the present study was to compare the different fixatives and post-fixative protocols on the quality of Masson trichrome staining. The canine intestines (n = 10), which were the duodenum and colon, were used as the candidate organs since they comprised of various tissue components. The fixatives and staining protocols were divided into four groups, which were Groups 1 and 2; …

Intradermal Vaccination With A Phytoglycogen Nanoparticle And Sting Agonist Induces Cytotoxic T Lymphocyte-Mediated Antitumor Immunity, Juan F. Hernandez-Franco, Imran M. Jan, Bennett D. Elzey, Harm Hogenesch

Purdue University Libraries Open Access Publishing Fund

A critical aspect of cancer vaccine development is the formulation with effective adjuvants. This study evaluated whether combining a cationic plant-derived nanoparticle adjuvant (Nano-11) with the clinically tested STING agonist ADU-S100 (MIW815) could stimulate anticancer immunity by intradermal vaccination. Nano-11 combined with ADU-S100 (NanoST) synergistically activated antigen-presenting cells, facilitating protein antigen cross-presentation in vitro and in vivo. Intradermal vaccination using ovalbumin (OVA) as a tumor antigen and combined with Nano-11 or NanoST prevented the development of murine B16-OVA melanoma and E.G7-OVA lymphoma tumors. The antitumor immunity was abolished by CD8+ T cell depletion but not by CD4+ T cell depletion. …

Seroprevalence Of Lawsonia Intracellularis Antibodies Among Large-Scale Swine Farming Systems In Thailand, Thanh Che Nguyen, Chairani Ridha Maghfira, Nathaya Young, Kanokwan Samatiwat, Chaiyan Kaewpaluk, Apisak Laothanakit, Pawarisa Mungprasittichai, Roongroje Thanawongnuwech, Yaowalak Panyasing

The Thai Journal of Veterinary Medicine

Gastrointestinal infectious diseases caused by bacteria have been a major problem in the swine industry, including Thailand. Porcine proliferative enteropathy (PPE), also known as porcine ileitis, is an enteric infectious disease caused by Lawsonia intracellularis – an obligate intracellular bacterium. PPE has been an economic threat to the global pork production system due to various types of diarrheas. Serological diagnosis can be applied to the seroprevalence of pathogen-antibodies and the timing of L. intracellularis infection when introduced to the herd. This present study showed the current serological status and the seroprevalence of antibodies against L. intracellularis in large-scale Thai swine …

Effects Of Prostaglandin Administration After Farrowing On Milk Output, Vaginal Discharge, And Mastitis During The Early Postpartum Period In Sows Under Field Conditions, G. Romano, R. Saleri, F. De Rensis, E. Dall’Olio, Padet Tummaruk, M. Andrani, C. Mazzoni

The Thai Journal of Veterinary Medicine

The present study evaluates the clinical effects of early post-partum prostaglandin administration on milk output, vaginal discharge and mastitis in sow. Two groups of sows were used: sows (n = 25) treated with prostaglandin F-2alpha (PGF-2α) intramuscularly 36 h after farrowing and a control group (n = 48) that did not receive any treatment. Seventy-two hours after farrowing, all animals were scored for rectal temperature, respiratory rate, feed ingestion, milk output, vaginal discharge and mastitis. Rectal temperature, feed intake, and milk output did not differ between the groups (P = 0.43). Regarding the clinical data, vaginal discharge was detected in …

A History Of The Development Of Alternatives To Animals In Research And Testing, John Parascandola

New Directions in the Human-Animal Bond

Growing public interest in animal welfare issues in recent decades has prompted increased attention to the efforts to develop alternative, nonanimal methods for use in biomedical research and product testing. In A History of the Development of Alternatives to Animals in Research and Testing, the first book-length study of the subject, John Parascandola traces the history of the concept of alternatives to the use of animals in research and testing in Britain and the United States from its beginnings until it had become firmly established in the scientific and animal protection communities by the end of the 1980s. This …

Concepts In Animal Parasitology, Scott L. Gardner, Sue Ann Gardner

Zea E-Books Collection

Note that this version of the textbook has been superceded by the one updated in 2026, available at the UNL Pressbooks site.

The hard copy version that is for sale at lulu.com is the old version.

"This is a textbook covering concepts in animal parasitology. It is meant to be used by students, teachers, professors, researchers, and members of the public who are interested in learning about animal parasite biology, systematics, taxonomy, zoogeography, and ecology. The primary intended audience is upper-level undergraduate or graduate university students who have knowledge of basic biology and, particularly, basic animal biology. (863 pages, …

Targeting Cancers With Ohsv-Based Oncolytic Viral Immunotherapy, Rakin Tammam Nasar, Ifeanyi Kingsley Uche, Konstantin G. Kousoulas

School of Medicine Faculty Publications

The recent success of cancer immunotherapies, such as immune checkpoint inhibitor (ICIs), monoclonal antibodies (mAbs), cancer vaccines, and adoptive cellular therapies (ACTs), has revolutionized traditional cancer treatment. However, these immunotherapeutic modalities have variable efficacies, and many of them exhibit adverse effects. Oncolytic viral Immunotherapy (OViT), whereby viruses are used to directly or indirectly induce anti-cancer immune responses, is emerging as a novel immunotherapy for treating patients with different types of cancer. The herpes simplex virus type-1 (HSV-1) possesses many characteristics that inform its use as an effective OViT agents and remains a leading candidate. Its recent clinical success resulted in …

Screening For Potential Swine-Origin Probiotics With Antimicrobial Activities, Christina Lim

Animal Science Undergraduate Honors Theses

The prevalence of antibiotic resistant bacteria is a leading issue worldwide. Recently, there was a new law banning the use of antibiotics within the swine industry. For decades swine producers have mixed subtherapeutic amounts of antibiotics in swine feed to prevent potential infections and to increase growth rate. However, due to the constant exposure of antibiotics to these ever-evolving pathogens, the population of antibiotic resistant bacteria has increased exponentially. Currently, the swine industry is scrambling to find a suitable alternative to antibiotics that possesses antimicrobial activity and potentially promotes growth. The objective of this study was to investigate the ability …

Stem Cell Therapy For Canine And Feline Patients With Chronic Kidney Disease, Seth Clark

Honors College Theses

This research aimed to statistically analyze the relationship between stem cell therapy and chronic renal failure in a sample of canine and feline patients. Stem cell therapy is a burgeoning field in both human and veterinary medicine, seeking mainly to better understand disease incidence and repair damaged tissues, such as those involved in spinal cord injuries and Alzheimer’s disease. In relation to veterinary medicine, prior research suggests that stem cell therapy has potential to be an effective tool against diseases such as acute kidney injury and chronic kidney disease. This project examined how autologous adipose-derived mesenchymal stem cells affected the …

Pathology And Osteological Observations Of Early Pliocene Rhinoceros, Teleoceras Aepysoma (Perissodactyla, Rhinocerotidae) From Gray Fossil Site, Tennessee, Thomas Scaife

Electronic Theses and Dissertations

Rhinoceroses were an important part of North America’s Paleogene and Neogene ecosystems, with Teleoceras aepysoma being one of the last representatives of this family. Specimens of T. aepysoma from the Gray Fossil Site (GFS) possess distinct/peculiar pathologies: including a pair of fused ribs and ankylosed phalanges. A qualitative description of the pathologies in the GFS T. aepysoma, including new material, was conducted to accurately identify pathologies and make interpretations about the life history of the GFS rhinos. Analysis suggests that rheumatoid arthritis is common in the lower limb bones of GFS rhinos. Additionally, the rib and toe pathologies are more …

Cross-Sectional Study Of Personal Protective Equipment Use, Training And Biosafety Preparedness Among Healthcare Workers During The First Months Of The Sars-Cov-2 Pandemic In Brazil, Kerstin Muner, Julia Kilgour, Tatiana Ometta, Ana Perola Drulla Brandao, Andrea Pires Dos Santos, Ana Marcia Sa Guimaraes

Purdue University Libraries Open Access Publishing Fund

Objectives Brazil has high rates of COVID-19 and tuberculosis among healthcare workers (HCWs). Personal protective equipment (PPE) is essential for their protection. We aimed to evaluate PPE use, training, and preparedness among HCWs in the early months of the SARS-CoV-2 pandemic in Brazil.

Methods A cross-sectional study was performed using questionnaires available to HCWs through a website created to provide PPE guidelines. χ2 test and robust Poisson regression identified factors associated with HCWs treating COVID-19 patients (TCOVID-19), lack of training on PPE use and N95 respirator reuse. The speech content of open-ended questions was analysed.

Results We analysed 1410 questionnaires …

Identification Of Novel Small Molecule Inhibitors Of Twin Arginine Translocation (Tat) Pathway And Their Effect On The Control Of Campylobacter Jejuni In Chickens, Loïc Deblais, Mary Drozd, Anand Kumar, Janet Antwi, James Fuchs, Rahul Khupse, Yosra A. Helmy, Gireesh Rajashekara

School of Veterinary and Biomedical Sciences: Faculty Publications

Introduction: Control of Campylobacter from farm to fork is challenging due to the frequent emergence of antimicrobial-resistant isolates. Furthermore, poultry production systems are known reservoirs of Campylobacter. The twinarginine translocation (Tat) pathway is a crucial bacterial secretion system that allows Campylobacter to colonize the host intestinal tract by using formate as the main source of energy. However, Tat pathway is also a major contributing factor for resistance to copper sulfate (CuSO₄).

Methods: Since mammals and chickens do not have proteins or receptors that are homologous to bacterial Tat proteins, identification of small molecule (SM) inhibitors …

Isothiocyanates Potentiate Tazemetostat-Induced Apoptosis By Modulating The Expression Of Apoptotic Genes, Members Of Polycomb Repressive Complex 2, And Levels Of Tri-Methylating Lysine 27 At Histone 3 In Human Malignant Melanoma Cells, Ioannis Anestopoulos, Ioannis Paraskevaidis, Sotiris Kyriakou, Lambrini E. Giova, Dimitrios T. Trafalis, Sotiris Botaitis, Rodrigo Franco, Aglaia Pappa, Mihalis L. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

In this study, we utilized an in vitro model consisting of human malignant melanoma as well as non-tumorigenic immortalized keratinocyte cells with the aim of characterizing the therapeutic effectiveness of the clinical epigenetic drug Tazemetostat alone or in combination with various isothiocyanates. In doing so, we assessed markers of cell viability, apoptotic induction, and expression levels of key proteins capable of mediating the therapeutic response. Our data indicated, for the first time, that Tazemetostat caused a significant decrease in viability levels of malignant melanoma cells in a dose- and time-dependent manner via the induction of apoptosis, while non-malignant keratinocytes were …

Gene Editing Of Pigs To Control Influenza A Virus Infections, Taeyong Kwon, Bianca L. Artiaga, Chester D. Mcdowell, Kristin M. Whitworth, Kevin D. Wells, Randall S. Prather, Gustavo Delhon, Mark Cigan, Stephen N. White, Jamie Retallick, Natasha N. Gaudreault, Igor Morozov, Juergen A. Richt

School of Veterinary and Biomedical Sciences: Faculty Publications

Proteolytic activation of the hemagglutinin (HA) glycoprotein by host cellular proteases is pivotal for influenza A virus (IAV) infectivity. Highly pathogenic avian influenza viruses possess the multibasic cleavage site of the HA which is cleaved by ubiquitous proteases, such as furin; in contrast, the monobasic HA motif is recognized and activated by trypsin-like proteases, such as the transmembrane serine protease 2 (TMPRSS2). Here, we aimed to determine the effects of TMPRSS2 on the replication of pandemic H1N1 and H3N2 subtype IAVs in the natural host, the pig. The use of the CRISPR/Cas 9 system led to the establishment of homozygous …