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Veterinary Pathology and Pathobiology Commons™

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2005

Articles 1 - 9 of 9

Full-Text Articles in Veterinary Pathology and Pathobiology

5-Ht1b Receptor-Mediated Presynaptic Inhibition Of Gaba Release In The Suprachiasmatic Nucleus, Jayne R. Bramley, Patricia J. Sollars, Gary E. Pickard, F. Edward Dudek Feb 2005

5-Ht1b Receptor-Mediated Presynaptic Inhibition Of Gaba Release In The Suprachiasmatic Nucleus, Jayne R. Bramley, Patricia J. Sollars, Gary E. Pickard, F. Edward Dudek

School of Veterinary and Biomedical Sciences: Faculty Publications

The suprachiasmatic nucleus (SCN) receives a dense serotonergic innervation that modulates photic input to the SCN via serotonin 1B (5-HT1B) presynaptic receptors on retinal glutamatergic terminals. However, the majority of 5-HT1B binding sites in the SCN are located on nonretinal terminals and most axonal terminals in the SCN are GABAergic. We therefore tested the hypothesis that 5-HT1B receptors might also be located on SCN GABAergic terminals by examining the effects of the highly selective 5-HT1B receptor agonist CP-93,129 on SCN miniature inhibitory postsynaptic currents (mIPSCs). Whole cell patch-clamp recordings of mIPSCs were obtained from rat …

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Voluntary Exercise In The C57b1/6j Mouse: Phenotypic Effects Of Varying Dietary Fat Levels And Hippocampal Gene Expression Differences Between High-Level And Low-Level Exercisers, Leslie Debardeleben Mclaughlin Jan 2005

Voluntary Exercise In The C57b1/6j Mouse: Phenotypic Effects Of Varying Dietary Fat Levels And Hippocampal Gene Expression Differences Between High-Level And Low-Level Exercisers, Leslie Debardeleben Mclaughlin

LSU Doctoral Dissertations

The drive to exercise voluntarily likely results from complex interactions between genes in many organ systems and various psychological parameters, such as motivation and the perception of fatigue. Reproducible variations in exercise intensity and duration are well established in laboratory rodents, but the genes responsible remain largely unknown. Also, to date, studies addressing the adaptive changes to exercise that might prevent dietary-induced obesity have focused primarily on energy intake and nutrient oxidation/partitioning, as opposed to genetics. We hypothesize that increased voluntary physical activity may be a normal mechanism in certain rodent strains to deter dietary-induced obesity and that in an …

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Neural Stem Cells As A Delivery Vector For Chemokine Expression In The Central Nervous System, Mark Winston Stalder Jan 2005

Neural Stem Cells As A Delivery Vector For Chemokine Expression In The Central Nervous System, Mark Winston Stalder

LSU Master's Theses

Increased expression of cytokines and chemokines in the central nervous system (CNS) is closely associated with the development of retroviral-induced neurological diseases such as HIV-associated dementia, as well as other neuropathologies such as Alzheimer’s Disease and Multiple Sclerosis. The specific functions of many of these pro-inflammatory factors have yet to be elucidated in the disease process, and it is unclear whether the nature of their effects is protective, pathogenic, or both. Additionally, current models of chemokine function have inherent limitations, with direct injection resulting in a brief response that doesn’t accurately represent the effects of chronic production, and transgenic mice …

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Role Of The Hypervariable Hinge Region Of Phosphoprotein P Of Vesicular Stomatitis Virus In Viral Rna Synthesis And Assembly Of Infectious Virus Particles, Subash C. Das, Asit K. Pattnaik Jan 2005

Role Of The Hypervariable Hinge Region Of Phosphoprotein P Of Vesicular Stomatitis Virus In Viral Rna Synthesis And Assembly Of Infectious Virus Particles, Subash C. Das, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein (P protein) of vesicular stomatitis virus (VSV) is an essential subunit of the viral RNAdependent RNA polymerase and has multiple functions residing in its different domains. In the present study, we examined the role of the hypervariable hinge region of P protein in viral RNA synthesis and recovery of infectious VSV by using transposon-mediated insertion mutagenesis and deletion mutagenesis. We observed that insertions of 19-amino-acid linker sequences at various positions within this region affected replication and transcription functions of the P protein to various degrees. Interestingly, one insertion mutant was completely defective in both transcription and replication. Using …

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Cutting Edge: Cd4+Cd25+ Regulatory T Cells Contribute To Gender Differences In Susceptibility To Experimental Autoimmune Encephalomyelitis, Jay Reddy, Hanspeter Waldner, Xingmin Zhang, Zsolt Illes, Kai W. Wucherpfennig, Raymond A. Sobel, Vijay K. Kuchroo Jan 2005

Cutting Edge: Cd4+Cd25+ Regulatory T Cells Contribute To Gender Differences In Susceptibility To Experimental Autoimmune Encephalomyelitis, Jay Reddy, Hanspeter Waldner, Xingmin Zhang, Zsolt Illes, Kai W. Wucherpfennig, Raymond A. Sobel, Vijay K. Kuchroo

Jay Reddy Publications

Female B10.S mice are highly resistant to proteolipid protein (PLP) 139–151-induced experimental autoimmune encephalomyelitis (EAE) and depletion of PLP 139–151- reactive CD4+CD25+regulatory T (Treg) cells can slightly increase their EAE susceptibility. Although male B10.S mice are moderately susceptible to EAE, we report that depletion of Treg cells in male B10.S mice before immunization with PLP 139–151 renders them highly susceptible to severe EAE with more CNS neutrophil infiltrates than nondepleted controls. Increased susceptibility is associated with an enhanced PLP 139–151-specific T cell response and greater production of IFN-γ, IL-6, and IL-17. Male CD4+CD25+ effector cells depleted of Treg cells proliferate …

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Development And Pathogenesis Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) In Experimentally Infected Thinhorn Sheep (Ovis Dalli), Emily J. Jenkins, Eric P. Hoberg, L. Polley Jan 2005

Development And Pathogenesis Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) In Experimentally Infected Thinhorn Sheep (Ovis Dalli), Emily J. Jenkins, Eric P. Hoberg, L. Polley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Recently, the protostrongylid nematode Parelaphostrongylus odocoilei has been reported in a new host species, thinhorn sheep (Ovis dalli). For the first time, we completed the life cycle of P. odocoilei in three Stone’s sheep (O. dalli stonei) and two thinhorn hybrids (O. dalli stonei × O. dalli dalli), each infected with 200 third-stage larvae from slugs (Deroceras laeve). The prepatent period ranged from 68 days to 74 days, and shedding of first-stage larvae (L1) peaked at >10,000 L1 per gram of feces between 90 and 110 days postinfection. A total of 75, …

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Cytokines And Aeroallergens In The Pathogenesis Of Summer-Pasture Associated Obstructive Pulmonary Disease: Effects On Endothelin Production, Neutrophil Activation And Chemotaxis, Lais Rosa Rodrigues Costa Jan 2005

Cytokines And Aeroallergens In The Pathogenesis Of Summer-Pasture Associated Obstructive Pulmonary Disease: Effects On Endothelin Production, Neutrophil Activation And Chemotaxis, Lais Rosa Rodrigues Costa

LSU Doctoral Dissertations

Summer pasture-associated obstructive pulmonary disease (SPAOPD), a naturally occurring airway disease of horses, is characterized by clinical exacerbation associated with exposure to pasture environment during the summer. Aeroallergens are believed to trigger exacerbation of SPAOPD, cytokines are likely associated with the anamnestic response to aeroallergens, and endothelin (ET)-1 is a potential mediator of airway obstruction. The goal of this dissertation was to describe and explore the interaction of aeroallergens triggering inflammation and T lymphocytes cytokine profile with the recruitment and activation of neutrophils and synthesis of ET-1 by mononuclear leukocytes and airway epithelial cells. The temporal pattern of clinical exacerbation …

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Genetics And Functions Of The Sars Coronavirus Spike Protein, Chad Michael Petit Jan 2005

Genetics And Functions Of The Sars Coronavirus Spike Protein, Chad Michael Petit

LSU Doctoral Dissertations

The SARS-Coronavirus (SARS-CoV) is the etiological agent of the severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. Investigations, described herein, have focused on the genetic manipulation of the SARS-CoV S glycoprotein in order to delineate functional domains within the protein. This was accomplished by incorporating single point mutations, cluster-to-lysine and cluster-to-alanine mutations, as well as carboxyl terminal truncations into the protein and investigating these mutants in transient expression experiments. Mutagenesis of either the coiled-coil domain of the S glycoprotein amino terminal heptad repeat, the predicted fusion peptide, …

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The Role Of Urease In The Pathogenesis Of Edwardsiella Ictaluri, Natha Joy Booth Jan 2005

The Role Of Urease In The Pathogenesis Of Edwardsiella Ictaluri, Natha Joy Booth

LSU Doctoral Dissertations

An Edwardsiella ictaluri strain with disruption of ureG was identified through the use of signature tagged insertion mutagenesis as being attenuated for virulence in the channel catfish host. Sequencing of the flanking regions surrounding the insert showed that the gene was part of a urease gene complex that included ureE, ureF, ureG, ureD, ureI, and an ammonium transporter homologue. The ureG gene encodes a GTP-binding accessory protein which is thought to function in energy-dependent urease assembly. The ureG mutant strain was found to be attenuated for mortality, persistence, and for the ability to establish infection in a competition challenge during …

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