Veterinary Pathology and Pathobiology Commons^™

Intradermal Vaccination With A Phytoglycogen Nanoparticle And Sting Agonist Induces Cytotoxic T Lymphocyte-Mediated Antitumor Immunity, Juan F. Hernandez-Franco, Imran M. Jan, Bennett D. Elzey, Harm Hogenesch

Purdue University Libraries Open Access Publishing Fund

A critical aspect of cancer vaccine development is the formulation with effective adjuvants. This study evaluated whether combining a cationic plant-derived nanoparticle adjuvant (Nano-11) with the clinically tested STING agonist ADU-S100 (MIW815) could stimulate anticancer immunity by intradermal vaccination. Nano-11 combined with ADU-S100 (NanoST) synergistically activated antigen-presenting cells, facilitating protein antigen cross-presentation in vitro and in vivo. Intradermal vaccination using ovalbumin (OVA) as a tumor antigen and combined with Nano-11 or NanoST prevented the development of murine B16-OVA melanoma and E.G7-OVA lymphoma tumors. The antitumor immunity was abolished by CD8+ T cell depletion but not by CD4+ T cell depletion. …

A History Of The Development Of Alternatives To Animals In Research And Testing, John Parascandola

New Directions in the Human-Animal Bond

Growing public interest in animal welfare issues in recent decades has prompted increased attention to the efforts to develop alternative, nonanimal methods for use in biomedical research and product testing. In A History of the Development of Alternatives to Animals in Research and Testing, the first book-length study of the subject, John Parascandola traces the history of the concept of alternatives to the use of animals in research and testing in Britain and the United States from its beginnings until it had become firmly established in the scientific and animal protection communities by the end of the 1980s. This …

Cross-Sectional Study Of Personal Protective Equipment Use, Training And Biosafety Preparedness Among Healthcare Workers During The First Months Of The Sars-Cov-2 Pandemic In Brazil, Kerstin Muner, Julia Kilgour, Tatiana Ometta, Ana Perola Drulla Brandao, Andrea Pires Dos Santos, Ana Marcia Sa Guimaraes

Purdue University Libraries Open Access Publishing Fund

Objectives Brazil has high rates of COVID-19 and tuberculosis among healthcare workers (HCWs). Personal protective equipment (PPE) is essential for their protection. We aimed to evaluate PPE use, training, and preparedness among HCWs in the early months of the SARS-CoV-2 pandemic in Brazil.

Methods A cross-sectional study was performed using questionnaires available to HCWs through a website created to provide PPE guidelines. χ2 test and robust Poisson regression identified factors associated with HCWs treating COVID-19 patients (TCOVID-19), lack of training on PPE use and N95 respirator reuse. The speech content of open-ended questions was analysed.

Results We analysed 1410 questionnaires …

Nuclear Phosphoproteome Reveals Prolyl Isomerase Pin1 As A Modulator Of Oncogene-Induced Senescence, Rodrigo Mohallem, Uma K. Aryal

Purdue University Libraries Open Access Publishing Fund

Mammalian cells possess intrinsic mechanisms to prevent tumorigenesis upon deleterious mutations, including oncogene-induced senescence (OIS). The molecular mechanisms underlying OIS are, however, complex and remain to be fully characterized. In this study, we analyzed the changes in the nuclear proteome and phosphoproteome of human lung fibroblast IMR90 cells during the progression of OIS induced by oncogenic RASG12V activation. We found that most of the differentially regulated phosphosites during OIS contained prolyl isomerase PIN1 target motifs, suggesting PIN1 is a key regulator of several promyelocytic leukemia nuclear body proteins, specifically regulating several proteins upon oncogenic Ras activation. We showed that PIN1 …

“Dying Alone And Being Eaten”: Dog Scavenging On The Remains Of An Elderly Animal Hoarder-A Case Report, Louise Bach Kmetiuk, Paulo Cesar Maiorka, Alan M. Beck, Alexander Welker Biondo

Purdue University Libraries Open Access Publishing Fund

Animal hoarding and human consumption by dogs have been important but often understudied aspects of the human-animal bond that can be addressed within a One Health framework. No scientific report has focused on dog scavenging on animal hoarders to date, despite isolated reports of dog scavenging on human remains, mostly due to starvation. The phenomenon has been approached as a confounding factor for human forensics. In 2014, the Animal Protection Department of Curitiba City was called to rescue and handle ten small dogs which had scavenged for a week on the human remains of their elderly owner, a potential animal …

Assistance Dogs For Military Veterans With Ptsd: A Systematic Review, Meta-Analysis, And Meta-Synthesis, Sarah C. Leighton, Leanne O. Nieforth, Marguerite E. O'Haire

Purdue University Libraries Open Access Publishing Fund

Psychiatric assistance dogs for military veterans with posttraumatic stress disorder (PTSD) currently make up over 19% of assistance dog partnerships globally. We conducted a systematic review of the literature relating to these partnerships, with specific aims to (1) summarize their characteristics, (2) evaluate the quality of existing evidence, and (3) summarize outcomes. A total of 432 records were independently screened (Cohen’s kappa = 0.90). Of these, 41 articles (29 peer-reviewed publications and 12 unpublished dissertations) met inclusion criteria. Data extraction was conducted to address the research aims, including a meta-analysis (quantitative outcomes) and meta-synthesis (qualitative outcomes). All peer-reviewed publications on …

Pioneer Science And The Great Plagues: How Microbes, War, And Public Health Shaped Animal Health, Norman F. Cheville, Purdue University Press

Purdue University Press Books

Pioneer Science and the Great Plagues covers the century when infectious plagues—anthrax, tuberculosis, tetanus, plague, smallpox, and polio—were conquered, and details the important role that veterinary scientists played. The narrative is driven by astonishing events that centered on animal disease: the influenza pandemic of 1872, discovery of the causes of anthrax and tuberculosis in the 1880s, conquest of Texas cattle fever and then yellow fever, German anthrax attacks on the United States during World War I, the tuberculin war of 1931, Japanese biological warfare in the 1940s, and today’s bioterror dangers.

Veterinary science in the rural Midwest arose from agriculture, …

Structural Disruption Of The Blood–Brain Barrier In Repetitive Primary Blast Injury, Gozde Uzunalli, Seth Herr, Alexandra M. Dieterly, Riyi Shi, L. Tiffany Lyle

Purdue University Libraries Open Access Publishing Fund

Background

Blast-induced traumatic brain injury (bTBI) is a growing health concern due to the increased use of low-cost improvised explosive devices in modern warfare. Mild blast exposures are common amongst military personnel; however, these women and men typically do not have adequate recovery time from their injuries due to the transient nature of behavioral symptoms. bTBI has been linked to heterogeneous neuropathology, including brain edema, neuronal degeneration and cognitive abnormalities depending on the intensity of blast overpressure and frequency. Recent studies have reported heterogeneity in blood–brain barrier (BBB) permeability following blast injury. There still remains a limited understanding of the …

Do Animals Engage Greater Social Attention In Autism? An Eye Tracking Analysis, Georgitta J. Valiyamattam, Harish Katti, Vinay K. Chaganti, Marguerite E. O'Haire, Virender Sachdeva

Purdue University Libraries Open Access Publishing Fund

Background: Visual atypicalities in autism spectrum disorder (ASD) are a well documented phenomenon, beginning as early as 2–6 months of age and manifesting in a significantly decreased attention to the eyes, direct gaze and socially salient information. Early emerging neurobiological deficits in perceiving social stimuli as rewarding or its active avoidance due to the anxiety it entails have been widely purported as potential reasons for this atypicality. Parallel research evidence also points to the significant benefits of animal presence for reducing social anxiety and enhancing social interaction in children with autism. While atypicality in social attention in ASD has been …

Laboratory Animal Welfare And Human Attitudes: A Cross-Sectional Survey On Heterospecific Play Or “Rat Tickling”, Megan R. Lafollette, Sylvie Cloutier, Colleen Brady, Brianna N. Gaskill, Marguerite E. O'Haire

Purdue University Libraries Open Access Publishing Fund

Introduction

Laboratory rat welfare is critically influenced by laboratory animal personnel through their implementation, or lack of implementation, of various enrichment techniques. One such promising technique is heterospecific play, or “rat tickling”, which mimics aspects of rat rough-and-tumble play and can contribute to improving welfare, but may be infrequently implemented. The theory of planned behavior can be used to study implementation by measuring intentions and beliefs about rat tickling, including behavioral attitudes (whether it is good or bad), subjective norms (whether there is social/professional pressure to provide it), and control beliefs (whether they feel in control of providing it). Therefore, …

Repurposing Celecoxib As A Topical Antimicrobial Agent, Shankar Thangamani, Waleed Younis, Mohamed N. Seleem

Department of Comparative Pathobiology Faculty Publications

There is an urgent need for new antibiotics and alternative strategies to combat multidrug-resistant bacterial pathogens, which are a growing clinical issue. Repurposing existing approved drugs with known pharmacology and toxicology is an alternative strategy to accelerate antimicrobial research and development. In this study, we show that celecoxib, a marketed inhibitor of cyclooxygenase-2, exhibits broad-spectrum antimicrobial activity against Gram-positive pathogens from a variety of genera, including Staphylococcus, Streptococcus, Listeria, Bacillus, and Mycobacterium, but not against Gram-negative pathogens. However, celecoxib is active against all of the Gram-negative bacteria tested, including strains of, Acinetobacter, and Pseudomonas, when …

Antibacterial Activity Of Novel Cationic Peptides Against Clinical Isolates Of Multi-Drug Resistant Staphylococcus Pseudintermedius From Infected Dogs, Mohamed F. Mohamed, G. Kenitra Hammac, Lynn F. Guptil, Mohamed N. Seleem

Department of Comparative Pathobiology Faculty Publications

Staphylococcus pseudintermedius is a major cause of skin and soft tissue infections in companion animals and has zoonotic potential. Additionally, methicillin-resistant S. pseudintermedius (MRSP) has emerged with resistance to virtually all classes of antimicrobials. Thus, novel treatment options with new modes of action are required. Here, we investigated the antimicrobial activity of six synthetic short peptides against clinical isolates of methicillin-susceptible and MRSP isolated from infected dogs. All six peptides demonstrated potent antistaphylococcal activity regardless of existing resistance phenotype. The most effective peptides were RRIKA (with modified C terminus to increase amphipathicity and hydrophobicity) and WR-12 (α-helical peptide consisting exclusively …

Mycoplasma Haemocanis – The Canine Hemoplasma And Its Feline Counterpart In The Genomic Era, Naila C. Do Nascimento, Andrea P. Santos, Ana Ms Guimaraes, Phillip J. Sanmiguesl, Joanne Messick

Department of Comparative Pathobiology Faculty Publications

Mycoplasma haemocanis is a hemotrophic mycoplasma (hemoplasma), blood pathogen that may cause acute disease in immunosuppressed or splenectomized dogs. The genome of the strain Illinois, isolated from blood of a naturally infected dog, has been entirely sequenced and annotated to gain a better understanding of the biology of M. haemocanis. Its single circular chromosome has 919 992 bp and a low G + C content (35%), representing a typical mycoplasmal genome. A gene-by-gene comparison against its feline counterpart, M. haemofelis, reveals a very similar composition and architecture with most of the genes having conserved synteny extending over their entire chromosomes …

The Effect Of Infection Order Of Porcine Circovirus Type 2 And Porcine Reproductive And Respiratory Syndrome Virus On Dually Infected Swine Alveolar Macrophages, Yi-Chieh Tsai, Hui-Wen Chang, Chian-Ren Jeng, Tsang L. Lin, Chun-Ming Lin, Cho-Hua Wan, Victor Fei Pang

Department of Comparative Pathobiology Faculty Publications

Abstract Background: Concurrent infection with porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) is known as one of the major causes for porcine respiratory disease complex (PRDC). Dual infection with PCV2 and PRRSV is consistently to have more severe clinical presentations and pulmonary lesions than infection with PCV2 alone or PRRSV alone. However, it is not known if dual infections with PCV2 and PRRSV in different infection order may lead to different clinical symptoms in the host. To mimic the possible field conditions, swine alveolar macrophages (AMs) were inoculated with PCV2 and PRRSV in vitro …

Zwitterionic Chitosan Derivative, A New Biocompatible Pharmaceutical Excipient, Prevents Endotoxin-Mediated Cytokine Release, Gaurav Bajaj, William G. Van Alstine, Yoon Yeo

Department of Comparative Pathobiology Faculty Publications

Chitosan is a cationic polymer of natural origin and has been widely explored as a pharmaceutical excipient for a broad range of biomedical applications. While generally considered safe and biocompatible, chitosan has the ability to induce inflammatory reactions, which varies with the physical and chemical properties. We hypothesized that the previously reported zwitterionic chitosan (ZWC) derivative had relatively low pro-inflammatory potential because of the aqueous solubility and reduced amine content. To test this, we compared various chitosans with different aqueous solubilities or primary amine contents with respect to the intraperitoneal (IP) biocompatibility and the propensity to induce proinflammatory cytokine production …

Genome Of Mycoplasma Haemofelis, Unraveling Its Strategies For Survival And Persistence, Andrea P. Santos, Ana Ms Guimaraes, Naila C. Do Nascimento, Phillip J. Sanmiguel, Samuel W. Martin, Joanne Messick

Department of Comparative Pathobiology Faculty Publications

Mycoplasma haemofelis is a mycoplasmal pathogen (hemoplasma) that attaches to the host’s erythrocytes. Distributed worldwide, it has a significant impact on the health of cats causing acute disease and, despite treatment, establishing chronic infection. It might also have a role as a zoonotic agent, especially in immunocompromised patients. Whole genome sequencing and analyses of M. haemofelis strain Ohio2 was undertaken as a step toward understanding its survival and persistence. Metabolic pathways are reduced, relying on the host to supply many of the nutrients and metabolites needed for survival. M. haemofelis must import glucose for ATP generation and ribose derivates for …

Complete Genome Sequence Of Mycoplasma Suis And Insights Into Its Biology And Adaption To An Erythrocyte Niche, Ana Ms Guimaraes, Andrea P. Santos, Phillip Sanmiguel, Thomas Walter, Jorge Timenetsky, Joanne Messick

Department of Comparative Pathobiology Faculty Publications

Mycoplasma suis, the causative agent of porcine infectious anemia, has never been cultured in vitro and mechanisms by which it causes disease are poorly understood. Thus, the objective herein was to use whole genome sequencing and analysis of M. suis to define pathogenicity mechanisms and biochemical pathways. M. suis was harvested from the blood of an experimentally infected pig. Following DNA extraction and construction of a paired end library, whole-genome sequencing was performed using GS-FLX (454) and Titanium chemistry. Reads on paired-end constructs were assembled using GS De Novo Assembler and gaps closed by primer walking; assembly was validated by …

Spontaneous Feline Mammary Intraepithelial Lesions As A Model For Human Estrogen Receptor- And Progesterone Receptor-Negative Breast Lesions, Giovanni P. Burrai, Sulma Mohammed, Margaret A. Miller, Vincenzo Marras, Salvatore Pirino, Maria F. Addis, Sergio Uzzau, Elisabetta Antuofermo

Department of Comparative Pathobiology Faculty Publications

Background: Breast cancer is the most frequently diagnosed cancer in women. Intraepithelial lesions (IELs), such as usual ductal hyperplasia (UH), atypical ductal hyperplasia (ADH), and ductal carcinoma in situ (DCIS) are risk factors that predict a woman’s chance of developing invasive breast cancer. Therefore, a comparative study that establishes an animal model of pre-invasive lesions is needed for the development of preventative measures and effective treatment for both mammary IELs and tumors. The purpose of this study was to characterize the histologic and molecular features of feline mammary IELs and compare them with those in women. Methods: Formalin-fixed, paraffin-embedded specimens …

Epithelial Expression Of Mrna And Protein For Il-6, Il-10 And Tnf-Α In Endobronchial Biopsies In Horses With Recurrent Airway Obstruction, Miia Riihimaki, Amanda Raine, Jamshid Pourazar, Thomas Sandstrom, Tatiana Art, Pierre Lekeux, Laurent Couetil, John Pringle

Department of Comparative Pathobiology Faculty Publications

Page 1 of 7 (page number not for citation purposes) BMC Veterinary Research Research article Open Access Epithelial expression of mRNA and protein for IL-6, IL-10 and TNF-α in endobronchial biopsies in horses with recurrent airway obstruction Miia Riihimäki*1,6, Amanda Raine2, Jamshid Pourazar3, Thomas Sandström3, Tatiana Art4, Pierre Lekeux4, Laurent Couëtil5 and John Pringle1 Address: 1Department of Clinical Sciences, Equine Internal Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden, 2Dept. of Cell & Molecular Biology, Biomedical Centre, Uppsala, Sweden, 3Department of Respiratory Medicine and Allergy, University Hospital, Umeå, Sweden, 4Equine Sports Medicine Centre, Faculty of Veterinary Medicine, University of Liège, …