Physiology Commons^™

Regular Physical Activity Is Correlated With A Reduced Frequency Of Senescent T-Lymphocytes In Middle-Aged Men, Guillaume Spielmann, Brian K. Mcfarlin, Paula J.W. Smith, Keith Guy, Richard J. Simpson

International Journal of Exercise Science: Conference Proceedings

No abstract provided.

Age-Related Differences In Monocyte Toll-Like Receptor 2/4 And Cd54 Expression In Mice, Kelley Strohacker, Whitney L. Breslin, Brian K. Mcfarlin

International Journal of Exercise Science: Conference Proceedings

No abstract provided.

The Muscular Benefits Of 13 Weeks Of Functional Electrical Stimulation Cycling After Spinal Cord Injury, John Ward, Stanley Fisher, Adam Thrasher

International Journal of Exercise Science: Conference Proceedings

No abstract provided.

The Relationship Between Body Image And Physical Activity In African American Women, Scherezade K. Mama, Beth E. Quill Mph, Maria E. Fernandez-Esquer Phd, Jacqueline Y. Reese-Smith, Jorge A. Banda, Rebecca E. Lee

International Journal of Exercise Science: Conference Proceedings

No abstract provided.

Sphingolipid-Induced Activation Of The Volume-Sensitive Cl− Current Is Mediated By Mitochondrial Reactive Oxygen Species, Frank Raucci

Theses and Dissertations

Swelling-activated Cl− current (ICl,swell) is an outwardly-rectifying current that plays an important role in cardiac electrical activity, cellular volume regulation, apoptosis, and acts as a potential effector of mechanoelectrical feedback. Persistent activation of ICl,swell has been observed in a number of models of cardiovascular disease. Previously we showed that angiotensin II (Ang II), endothelin-1 (ET-1), endothelial growth factor receptor (EGFR), and reactive oxygen species (ROS) produced by NADPH oxidase (NOX) and mitochondria are involved in the activation of ICl,swell by both osmotic swelling and Beta1 integrin stretch. Sphingolipid metabolism is modulated in several cardiopathologies and because sphingolipids are bioactive lipids …

Multiple Models Can Concurrently Explain Fatigue During Human Performance, C. Matthew Laurent†, Matt Green‡

International Journal of Exercise Science

One of the most commonly and thoroughly studied paradigms of human performance is fatigue. However, despite volumes of research there remains considerable controversy among scientists regarding definitive conclusions about the specific mechanism(s) contributing to fatigue. Within the literature there are three primary yet distinctly different governing ideas of fatigue; the traditionally referenced central model and peripheral model as well as the emerging central governor model (CGM). The CGM has recently been advocated by a limited number of researchers and is suggestive of a more integrative model of fatigue when compared the traditional peripheral and central models. However, more work is …

Determination Of Force Corresponding To Maximal Lactate Steady State In Tethered Swimming, Marcelo Papoti‡, Ricardo Vitório†, Sérgio A. Cunha†, Gustavo G. Araújo†, Adelino S.R. Silva‡, Vanessa Santhiago‡, Luiz E.B. Martins‡, Claudio A. Gobatto‡

International Journal of Exercise Science

The main aim of the present investigation was to verify if the aerobic capacity (AC) measured in tethered swimming corresponds to the maximal lactate steady state (MLSS) and its correlation with 30 min and 400m free style swimming. Twenty-five swimmers were submitted to an incremental tethered swimming test (ITS) with an initial load of 20N and increments of 10N each 3min. After each stage of 3min, the athletes had 30s of interval to blood sample collections that were used to measure blood lactate concentrations ([La-]). The ACBI was determined by the abrupt increase in [La-] versus force (F). The points …

The Short-Term Effect Of Whole Body Vibration Training On Sprint Start In Collegiate Athletes, Brad Roberts*, Iain Hunter‡, Ty Hopkins‡, Brent Feland‡

International Journal of Exercise Science

Whole body vibration (WBV) is characterized by a vibratory stimulus emitted throughout the body through the use of a vibrating platform on which the subject stands. Studies have shown over 30% increases in maximal explosive strength such as maximal speed biceps curl as well as increases in maximum dynamic force such as maximal sitting bench pull as the result of vibration. The purpose of this study was to investigate the effects of short term whole-body vibration on sprint starts among collegiate track athletes. On the first day eleven subjects were randomly assigned to either a non-vibration or vibration group for …

Evaluation Of Hand-To-Hand Bioelectrical Impedance Analysis For Estimating Percent Body Fat In Young Adults, Aaron M. Weaver*, Ashley C. Hill*, Joseph L. Andreacci‡, Curt B. Dixon‡

International Journal of Exercise Science

Purposes were to (a) to examine the validity and precision of a hand-to-hand bioelectrical impedance analyzer (HBIA) and (b) to determine the effect of an acute sub-maximal aerobic exercise bout on HBIA percent body fat (%BF) measures. Forty-one young adults (21 women; 20 men) visited the laboratory for body composition assessment on two separate occasions. During the control session, %BF was assessed by HBIA twice, before and immediately after 30 min of rest, and once by air-displacement plethysmography (ADP), using the BOD POD, which was considered the criterion method for comparison. During the exercise session, HBIA %BF measurements were determined …

A Kinematic And Kinetic Case Study Of A Netball Shoulder Pass, Sharon A. Hetherington*, Sibella G. King*, Denis Visentin‡, Marie-Louise Bird‡

International Journal of Exercise Science

The majority of studies analysing netball skills using force platforms have focused on reducing the risk of injury from compression and torsion forces on the knee and ankle joints during landing and pivoting. In this preliminary case study our aim was to investigate the efficacy of a combination of tools to describe the kinematic and kinetic mechanisms underlying the netball shoulder pass. The segmental movements of the netball shoulder pass were analysed from video and force platform data in order to develop a suitable methodology for use in a larger study. Peak vertical ground reaction force of 850 N was …

Psychosocial Correlates Of Physical Activity In Children And Adolescents In A Rural Community Setting, Kelly Crimi†, Larry D. Hensley‡, Kevin J. Finn‡

International Journal of Exercise Science

The purpose of this study was to evaluate the relationships between selected psychosocial factors and the physical activity behaviors of children (grade 4-8) and adolescents (grades 9-12) in a rural community setting. The Children’s Physical Activity Scale (CPAC)was used to measure the psychosocial factors of physical activity, The Physical Activity Questionnaire-Children (PAQ-C), and Physical Activity Questionnaire-Adolescents (PAQ-A) were used to measure the physical activity behaviors of the 167 participants. Results indicated that male and female physical activity behaviors were not significantly different. However, physical activity declined with age [F(8,147) = 5.44, p < 0.05, ES = 0.23]. All psychosocial factors were significantly correlated with physical activity in youth with the single highest correlation for males being “liking of exercise” (r = .61) and the highest correlation for females was “liking of games and sports”(r = .44). Stepwise regression analyses identified three subscales (liking of games and sport, liking of exercise, and parental support) in a significant prediction model of physical activity in both genders. The results indicate that children's physical activity is associated with a variety of psychosocial variables that represent import predisposing and reinforcing factors.

Balance Across Boundaries: Service Learning From An International Perspective, Teresa C. Fitts

International Journal of Exercise Science

Service-Learning provides students, faculty and community partners an opportunity to go beyond classroom and laboratory instruction to the application of theoretical principles, and the development of civic responsibility by forging meaningful relationships with community partners. Service-learning also enables faculty to broaden the cultural sensitivity and international exposure of students by engaging students in directed, purposeful projects aimed at supporting the goals of both the college and the international partners. We have a unique opportunity in Exercise Science to make lasting changes and improve global conditions relating to student learning and the physical inactivity crisis. The purpose of this article is …

Motivational Profiles And Their Associations With Achievement Outcomes, Bo Shen, Nate Mccaughtry, Jeffrey J. Martin, Mariane Fahlman

Kinesiology, Health and Sport Studies

With the belief that theoretical integration in motivation may help us better understand motivational behavior, we designed this study to explore adolescents’ motivational profiles and their associations with knowledge acquisition, leisure-time exercise behaviors, and cardiorespiratory fitness. Middle school students from a large urban inner-city school district (N = 603, ages 12–14) completed questionnaires assessing motivational constructs and leisure-time exercise behavior. Knowledge and cardiorespiratory fitness were also assessed with a knowledge test and the Progressive Aerobic Cardiovascular Endurance Run (PACER) test, respectively. Using hierarchical cluster analysis, we found that students’ motivation in physical education could be explained from a multi-theoretical …

Prediction Of Birth Weight By Cotinine Levels During Pregnancy In A Population Of African American Smokers, Ayman A.E. El-Mohandes, Michele Kiely, Marie G. Gantz, Susan M. Blake, M Nabil El-Khorazaty

Publications and Research

Objective—The goal was to investigate the association between maternal salivary cotinine levels (SCLs) and pregnancy outcome among African Americans smokers

Methods—In a randomized controlled trial conducted in 2001-2004 in Washington, D.C. 714 women (126 active smokers (18%)) were tested for SCLs at the time of recruitment and later in pregnancy. Sociodemographic health risks and pregnancy outcomes were recorded.

Results—Birth weights were significantly lower for infants born to mothers with baseline SCLs of ≥20 ng/ml compared to/ml (p=0.024), ≥50 ng/ml compared to/ml (p=0.002), ≥100 ng/ml compared to/ml (p=0.002), in bivariate analyses. In linear regression analyses adjusting for sociodemographic …

Broad Range Amino Acid Specificity Of Rna-Dependent Lipid Remodelling By Multiple Peptide Resistance Factors, Hervé Roy, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Aminoacylphosphatidylglycerol synthases (aaPGSs) are multiple peptide resistance factors that transfer amino acids from aminoacyl-tRNAs to phosphatidylglycerol (PG) in the cytoplasmic membrane. Aminoacylation of PG is used by bacteria to decrease the net negative charge of the cell envelope, diminishing affinity for charged molecules and allowing for adaptation to environmental changes. Lys-PGS, which transfers lysine to PG, is essential for the virulence of certain pathogens, providing resistance to both host cationic antimicrobial peptides and therapeutic antibiotics. Ala-PGS was also recently described, but little is known about the possible activities of other members of the highly diverse aaPGS family of proteins. Systematic …

The Cca Anticodon Specifies Separate Functions Inside And Outside Translation In Bacillus Cereus, Sandro F. Ataide, Theresa E. Rogers, Michael Ibba

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Bacillus cereus 14579 encodes two tRNAs with the CCA anticodon, tRNA^Trp and tRNA^Other. tRNA^Trp was separately aminoacylated by two enzymes, TrpRS1 and TrpRS2, which share only 34% similarity and display different catalytic capacities and specificities. TrpRS1 was 18-fold more proficient at aminoacylating tRNA^Trp with Trp, while TrpRS2 more efficiently utilizes the Trp analog 5-hydroxy Trp. tRNA^Other was not aminoacylated by either TrpRS but instead by the combined activity of LysRS1 and LysRS2, which recognized sequence elements absent from tRNA^Trp. Polysomes were found to contain tRNA^Trp, consistent with its role in …

The Role Of Porphyromonas Gingivalis Lysine Specific Protease, Kgp In Hemin Transport And Pigment Accumulation, Anuj Phull

Theses and Dissertations

Porphyromonas gingivalis, a gram-negative anaerobic bacterium, is implicated as a major etiological agent in the initiation and progression of severe forms of periodontal disease. It has been reported that gingivitis and periodontal disease affect roughly 50.3% and 35% of the adult population, respectively. Therefore, approximately over 85% of the adult population may suffer from some form of gingival disease. Porphyromonas gingivalis, an established periodontopathogen, requires hemin for growth. Although multiple hemin uptake systems appear to be present in this organism, their specific role in hemin uptake and virulence remains unknown. Pigmentation is thought to result from the accumulation of iron …

Tumor Necrosis Factor-Alpha (Tnf-Α) Enhances Functional Thermal And Chemical Responses Of Trp Cation Channels In Human Synoviocytes, Mikhail Y. Kochukov, Terry A. Mcnearney, Huaizhi Yin, Liping Zhang, Fei Ma, Larissa Ponomareva, Sarah Abshire, Karin N. Westlund

Physiology Faculty Publications

Background: We have shown functional expression of several TRP channels on human synovial cells, proposing significance in known calcium dependent proliferative and secretory responses in joint inflammation. The present study further characterizes synoviocyte TRP expression and activation responses to thermal and osmotic stimuli after pre-treatment with proinflammatory mediator tumor necrosis factor alpha (TNF-α, EC50 1.3221 x 10^-10 g/L).

Results: Fluorescent imaging of Fura-2 loaded human SW982 synoviocytes reveals immediate and delayed cytosolic calcium oscillations elicited by (1) TRPV1 agonists capsaicin and resiniferatoxin (20-40% of cells), (2) moderate and noxious temperature change, and (3) osmotic stress TRPV4 activation (11.5% of …

Ion Channel Modulation By Photocaged Dioctanoyl Pip2, Junghoon Ha

Theses and Dissertations

Phosphatidylinositol bisphosphate (PIP2) directly regulates electrophysiological activity in a diverse family of ion channels whether the effect is stimulatory or inhibitory. Much has been unveiled about the apparent affinity and modulatory function of PIP2 using a chemically modified dioctanoyl PIP2 (diC8), a membrane delimited cytosolic co-factor in inside-out macropatch experiments. Yet, the scarcity of molecular tools that permit fine external control in whole-cell systems has precluded future studies from probing the physiological role of PIP2 in cells in the presence of a fully intact cytoplasm. Here we introduce light as an external control for PIP2 through photocaging of diC8, and …

Manipulating Testosterone To Assess Links Between Behavior, Morphology, And Performance In The Brown Anole Anolis Sagrei, Robert M. Cox, Derek S. Stenquist, Justin P. Henningsen, Ryan Calsbeek

Dartmouth Scholarship

Survival and reproductive success are determined by the complex interplay between behavior, physiology, morphology, and performance. When optimal trait combinations along these various phenotypic axes differ between sexes or across seasons, regulatory mechanisms such as sex steroids can often facilitate sex‐specific and/or seasonal trait expression. In this study, we used surgical castration and replacement of exogenous testosterone in adult male brown anoles (Anolis sagrei) to simultaneously examine the effects of testosterone on a suite of morphological (dewlap area, body size), physiological (immune function), behavioral (dewlap, head bob, and push‐up displays), and performance (stamina, sprint speed, bite force) traits. …

Molecular And Cellular Mechanisms Whereby The Apical Ectodermal Ridge (Aer), Via Wnt5a, Mediates Directional Migration Of The Adjacent Mesenchyme During Vertebrate Limb Development, Kate E. Kmetzsch

Theses and Dissertations

The vertebrate embryonic limb is a key model in elucidating the genetic basis underlying the three dimensional morphogenesis of structures. Despite the wealth of insights that have been generated from this model, many long-standing questions remain. For example, it has been known for over 70 years that the apical ectodermal ridge (AER) of the embryonic limb is essential for distal outgrowth and patterning of the adjacent limb mesenchyme. The mechanisms whereby the AER does accomplish outgrowth and patterning are still poorly understood. We propose that secreted FGFs from the AER activate Wnt5a expression in gradient fashion, which in turn provides …

Zyxin Regulates Epithelial-Mesenchymal Transition By Mediating Actin-Membrane Linkages At Cell-Cell Junctions, Liv Rebecca Sperry

Theses and Dissertations

Development is punctuated by morphogenetic rearrangements of epithelial tissues, including complete detachment of individual motile cells during epithelial-mesenchymal transition (EMT). Dramatic actin rearrangements occur as cell-cell junctions are dismantled and cells become independently motile during EMT. Characterizing dynamic actin rearrangements and identifying actin machinery driving these rearrangements is essential for understanding basic mechanisms of cell-cell junction remodeling; yet, neither the precise series of actin rearrangements at cell-cell junctions that accompany EMT, nor the machinery that controls actin rearrangement during EMT, have been identified. This work represents a detailed study of junctional actin reorganization in cells undergoing EMT, identifies actin regulatory …

Marrying Immunotherapy And Chemotherapy: A Cancer Therapy Based On T Lymphocyte Expansion Augmented By Alternate Gamma Chain Cytokines And Gemcitabine-Mediated Inhibition Of Myeloid Derived Suppressor Cells, Esther Cha

Theses and Dissertations

Successful adoptive immunotherapy (AIT) for cancer relies on the infusion of in vitro expanded, tumor-reactive lymphocytes with a goal of generating productive tumor immunity. Previously, our lab has developed a protocol to activate selectively tumor-reactive T lymphocytes in vitro using two pharmacologic agents, bryostatin-1 and ionomycin. Following the pharmacological stimulation, conventionally, IL-2 is added to stimulate in vitro proliferation. In this report, alternate cytokines from the common cytokine receptor γ-chain family, namely IL-7 and IL-15, were explored as the alternative cytokine supplements. We found that tumor DLN cells activated in vitro with B/I and cultured in IL-7/15 alternate common γ-chain …

The Effects Of Fluorescent Tracking Powder On Oxygen Consumption In Salamanders Using Either Cutaneous Or Bimodal Respiration, Sarah A. Orlofske, Kristine L. Grayson, William A. Hopkins

Biology Faculty Publications

Fluorescent powder is gaining attention as an effective method for tracking terrestrial amphibian movements, particularly for species that are too small for conventional tracking equipment. The technique requires coating portions of an animal with fluorescent powder, releasing the animal, and following the trail of powder as it is progressively lost during movement. Recent studies have shown that fluorescent powder has no negative effects on survival or growth. However, a substance that coats the skin, a major respiratory organ in most amphibians, may have sublethal effects on performance and consequently behavior. We tested the effect of fluorescent powder application on the …

The Effects Of Repeated Anaerobic Bouts On Immune Parameters, James C. Sivley

Masters Theses & Specialist Projects

To date, the exercise-induced immune response in has only been evaluated in subjects performing aerobic exercise. The primary purpose of this study is to determine if repeated bouts of anaerobic exercise will induce a similar immune response in human subjects as exhibited by aerobic exercise. Secondary to immune function, an analysis of performance from trial to trial will be made. Nine males between the ages of 18-25 were selected on a volunteer basis to participate in this study. Subjects performed Wingate tests set in 3 series with 120 seconds between trials within series and 405 seconds between series for an …

Adding Upstream Sequence And A Downstream Reporter To The Bile Acid Inducible Promoter Of Clostridium Scindens Vpi 12708, Bryan Patrick Mason

Masters Theses & Specialist Projects

Bile acids in the small intestines of animals serve to breakdown fats and fatsoluble vitamins. Most of the bile acids are reabsorbed into the enterohepatic circulation, but approximately five percent of these bile acids pass into the large intestine. These bile acids are swiftly deconjugated by the bacterial population, and then subjected to further intestinal bacterial chemical modifications. The most significant of these modifications are 7α-dehydroxylations which form secondary bile acids (deoxycholate and lithocholate). Much research has illuminated the 7α-dehydroxylation pathway: of particular interest is the bile acid inducible operon, for which Clostridium scindens VPI 12708 serves as the model …

Trunk Rotation Movement In Tennis Backhand, Hsin-Chen Fanchiang

All-Inclusive List of Electronic Theses and Dissertations

This study analyzed the effects of gender, strike technique, power level, and trial factors on tennis athletes performing backhand strokes with the incoming ball launched by a serving-stand. Five male and five female college tennis athletes volunteered as subjects. Each subject had 25 data points including joint centers of right and left sides for the 5th metatarsal, lateral malleolus, knees, hips, shoulders, elbows, wrists and MP joints, chin, forehead, seven points on the racket (handle, head, neck, upper right and left rims, and lower right and left rims), and the ball were recorded by two video cameras (60Hz). Each of …

Schizophrenia Candidate Genes Study, Grace Lee

Theses and Dissertations

Schizophrenia is a debilitating disorder caused by the interaction of genetic and environmental factors. In this study, we identified candidate genes and single nucleotide polymorphisms from two genome-wide association studies, GAIN and CATIE. Nine SNPs representing four candidate genes were selected for replication studies with our Irish samples: Irish Case-Control Study of Schizophrenia (ICCSS), the Irish Study of High-Density Schizophrenia Families (ISHDSF), and the Irish Trio Study of Schizophrenia (ITRIO). In the ITRIO sample, rs4704591 (CMYA5 gene) showed nominal significance (p = 0.0447947). Combining ICCSS, ISHDSF, and ITRIO samples for rs4704591 increased sample size and power and yielded a p-value …

Evaluation Of Association Of Microrna-122 With Histological Severity Of Recurrent Hcv Infection In Liver Transplant Recipients, Jihee Suh

Theses and Dissertations

Hepatitis C virus recurrence (which is defined by detection of HCV RNA in serum) in post-transplanted liver is universal but the progression of infection remains unpredictable, varying from case to case. It has been estimated that 75%-80% of the HCV recurrence patients will suffer chronic hepatitis C infection and up to a third of them will progress into the development of fibrosis and cirrhosis within 5 years post-transplantation. Therefore, finding ways to predict early on the progression of fibrosis can contribute to better prognoses. Recent literatures have mentioned that the hepatitis C virus relies on the host microRNA-122 (miR-122) for …

Comparison Of The Power Plate And Free Weight Exercises On Upper Body Muscle Endurance In Young Athletes, Elisabeth Boland*, Dan Boland*, Thomas Carroll‡, William R. Barfield‡

International Journal of Exercise Science

The power plate (PP) is designed to reduce training time while providing a muscle stimulus that leads to positive changes in muscle mass. This study investigated the effect that training on the PP has compared to a free-weight (FW) program, on upper body endurance, defined as the number of push-ups completed at one time prior to failure. Following IRB approval a pre-test was used to assess push-up endurance in PP and FW cohorts. Each group exercised for six consecutive weeks, working out three times per week, on non-consecutive days performing five exercises of two sets of 8-12 repetitions. Twenty-two females …