Physiology Commons^™

Comparison Of Recovery Methods After Resistance Exercise For Power Performance, Isaac D. Huerta

Theses and Dissertations

Elite athletes engage in intense training to secure marginal performance advantages, placing considerable physical strain on their bodies. Effective recovery techniques are critical to rapidly restore physical function. Conventional methods like ice baths, hydrotherapy, and massages are often impractical due to cost and logistical barriers. This study examined the efficacy of voluntary deep slow breathing (VDSB) as a cost-effective recovery method compared to static stretching (SS) and no recovery method (control) in untrained male athletes aged 18–26. Using a within-subjects, randomized crossover design over four weeks, participants visited the lab seven times to complete a standardized full-body workout involving plyometric …

Investigation Of The Hdl Molecular Architecture Before And After Exercise Training., Prasun Kumar Dev

Theses and Dissertations

The functional characteristics of high-density lipoprotein (HDL) particles are influenced by their structure, including their molecular composition (e.g. HDL lipidome and proteome). However, the effects of exercise training on HDL molecular composition and its relationship with HDL-related traits are largely unknown. In this ancillary study, we investigated the HDL lipidome and proteome of 154 adults before and after 20 weeks of exercise training in the HERITAGE Family Study. The HDL-sized plasma fraction was isolated utilizing FPLC-SEC, followed by untargeted lipidomic and proteomic analysis using LC/MS. We identified 341 known lipid species while 409 HDL associated proteins. Clinical and functional related …

The Effects Of Acute Nutrient Intake On Raw Segmental Bioelectrical Impedance Values And Fluid Shifts In Premenopausal Women, Callie Unrein

Theses and Dissertations

Background: Raw bioimpedance measures—impedance (Z), resistance (R), reactance (Xc), and phase angle (PhA)—are emerging tools to assess hydration, cellular integrity, and muscle quality. While multifrequency bioimpedance analysis (MF-BIA) can detect acute physiological shifts, its sensitivity to nutrient intake, especially across hormonal phases in women, remains underexplored. Purpose: This study investigated how essential amino acids (EAA), carbohydrates (CHO), and water (CON) influence postprandial segmental bioimpedance and fluid distribution in healthy women, and whether these responses vary by menstrual hormone phase. Methods: Twenty-two recreationally active women (18–40 years) underwent serial MF-BIA (InBody 970) assessments during high- and low-hormone phases, randomized to one …

Physiological Responses And Plant Trait Interactions With Coastal Dune And Swale Development, Emily C. Riffe

Theses and Dissertations

Vegetation is crucial to coastal ecosystems experiencing climate change and storm-induced disturbance. Plant species interact with the physical environment and are considered “ecosystem engineers.” Sand dunes and barrier islands are at the forefront of climate change impacts as they are barriers that protect landward habitat and communities. Nature based solutions are utilized to protect infrastructure on developed coastlines. Understanding how dominant plant species interact with and alter the physical environment enhances our understanding of the mechanisms of change and consequences to the system. The goal of this research was to quantify physiological and functional trait differences in dominant species found …

Caspases Protect Against Hyperglycemia Induced Necroptosis-Like Cell Death In Brain Endothelial Cells, Aleena S. Mathew

Theses and Dissertations

Acute diabetes characterized by high spikes in blood glucose levels in association with severe hyperglycemia can lead to an increased risk of ischemic stroke in the brain. To better understand high blood glucose levels, hyperglycemia, in the brain we explored its effects on endothelial cells. Endothelial cells line the inner walls of blood vessels and are directly impacted in hyperglycemia. Brain endothelial cells are a major component of the blood brain barrier (BBB) which tightly regulates the brain’s microenvironment. The type of cell death such as apoptosis, caspase dependent, or necroptosis, caspase independent, can exacerbate diabetes associated complications. There is …

Stress And Behavior In Beef Calves During Weaning, Kathleen E. Tierney

Theses and Dissertations

Animal welfare in commercial livestock operations has been an increasing topic of concern. It is well known that stress can have significant behavioral and physiological repercussions. It is especially important in commercial operations to reduce stress, as animal efficiency is the leading priority. In an effort to reduce stress on animals and improve welfare in livestock operations, several studies have evaluated common husbandry practices. One of the most widely studied practices is weaning, which encapsulates the process of removing the young from the dam. Several studies have evaluated the effects of weaning on beef cattle through a variety of behavioral …

Effects Of Metoprolol And Propranolol Beta-Blockers Mixture On Morphological And Physiological Responses In The American Oyster, Andrew Salinas

Theses and Dissertations

Beta-blockers are extensively used to treat human cardiovascular conditions yet pose significant risks to non-target aquatic organisms. This study aimed to investigate the effects of short-term exposure (one week) to a beta-blocker’s mixture (low-dose: 50 ng/L propranolol and 250 ng/L metoprolol, and high-dose: 250 ng/L propranolol and 650 ng/L metoprolol) in the American oysters. Histopathological assessments revealed structural damage to gills, connective tissues, and digestive glands in both low- and high-dose treatment groups. Additionally, glucose concentration and pH of the extrapallial fluid significantly declined in the high-dose treatment group. Hemocyte density within connective tissues increased proportionally with dosage. The beta-blocker …

Sphingosine Kinase 2 Depletion Dysregulates Hepatic Redox Metabolism And Enhances Inflammation In Early Metabolic Dysfunction-Associated Steatohepatitis (Mash), Kaitlyn Georgene Jackson

Theses and Dissertations

Metabolic dysfunction-associated steatotic liver disease (MASLD) currently affects one in three people worldwide; its prevalence is growing alongside other cardiometabolic disorders. Aberrations to redox and sphingolipid metabolism contribute to MASLD pathologic progression. Sphingosine-1-phosphate (S1P) is an active lipid, meaning that S1P participates in biochemical processes within the cell. S1P is generated by two distinct isoenzymes, sphingosine kinase (SphK)1 and SphK2. The localization of the SphK enzyme dictates S1P function. While cytosolic SphK1-generated S1P is well-characterized, nuclear and mitochondrial SphK2-generated S1P is less known. Moreover, SphK2 depletion effects on cellular redox processes and inflammation are not well-defined.

This study defines the …

Hypothalamic Manipulation Of The Anuran Hpg Axis: Alternative Hormones And A Non-Invasive Administration Route For Amphibian Assisted Reproductive Technologies (Art), Erin Michelle Saylor

Theses and Dissertations

Amidst the amphibian extinction crisis, in situ and ex situ amphibian species conservation initiatives utilize assisted reproductive technologies for optimal genetic management of captive and wild populations. Development of effective, simple, low-cost methods for obtaining gametes for artificial fertilization, sperm biobanking, or natural breeding alleviates obstacles for institutions or programs to apply reproductive technologies. Objectives herein include investigating the efficacy of alternative hormones for inducing spermiation, an alternative hormone administration route for inducing ovulation, exploring the physiological effects of hormone therapy, and utilizing sperm from deceased animals for biobanking.

Effects Of Short-Term Pesticides Exposure On Swimming Behavior, Tissue Morphology, Oxidative-Nitrative Stress, Antioxidant Expression, And Cellular Apoptosis In Kidneys Of Goldfish, Esmirna Cantú

Theses and Dissertations

The prevalence of pesticides in the aquatic environment continues to increase due to anthropogenic activities and poses a threat to aquatic organisms. The aim of this study was to determine the short-term exposure (one-week) of pesticide mixtures (metolachlor, linuron, isoproturon, tebucanazole, alconifen, atrazine, pendimethalin; azinphos-methyl) on swimming behaviors, kidney morphology, and expression of nitrotyrosine protein (NTP), dinitrophenyl protein (DNP), superoxide dismutase (SOD), catalase (CAT), renin, and cellular apoptosis in goldfish (Carassius auratus). Results indicated that both low- and high-dose pesticide mixtures decreased the distance swimming behavior. Histological analysis showed pesticide exposure alters kidney morphology. DNP, NTP, and renin expressions, and …

Effectiveness Of Intervention Studies Based On Diet And/Or Physical Activity In Treatment, Prevention, And Management Of Diabetes Among South Asians: A Systematic Review, Ishaan Arora

Theses and Dissertations

The purpose of this study was to describe diet and/or physical activity-based interventions and their control in cardiometabolic risk factors of diabetes for the South Asian (SA) population. A systematic review was conducted in accordance with the PRISMA guidelines. Seventeen randomized control trials meeting the inclusion criteria were included for analysis. Interventions with type-2 diabetic SA showed significant improvements in HbA1c% (4/5 studies), fasting blood glucose (3/4 studies), and postprandial glucose (2/2 studies). Interventions with at-risk/prediabetic and non-diabetic SA showed a modest change in overall outcomes, with significant change reported for post-prandial insulin (3/4 studies), BMI and weight (2/4 studies), …

Remote Physiological Monitoring Of The Giant Panda And Red Panda Using Near Infrared Spectroscopy And Machine Learning, Qingyu Sheng

Theses and Dissertations

Appropriate conservation decisions and efforts must be based on real−time and accurate information about wildlife populations. However, it is extremely challenging to monitor the population demography and physiological traits of many threatened and secretive animal species through direct observation and capture. Near infrared spectroscopy (NIRS) has the potential to be a remote tool to address questions concerning wildlife physiology and demography by analyzing “signs” of animals without seeing or capturing them. In this dissertation, two species, the giant panda (Ailuropoda melanoleuca) and red panda (Ailurus fulgens) are used as a case study, to demonstrate NIRS’ feasibility …

Effects Of Temperature On Growth, Metabolic Rate, And Lower Dissolved Oxygen Tolerance Of Speckled Peacock Bass Cichla Temensis, Manuel E. Coffill-Rivera

Theses and Dissertations

I examined the effects of temperature (25, 30, and 35°C) on growth, standard metabolic rate (SMR), and lower dissolved oxygen tolerance (LDOT) of juvenile Speckled Peacock Bass Cichla temensis. Fish were acclimated to 150-L aquaria for 7 weeks before the growth, SMR, and LDOT experiments. The growth study lasted 58 days and fish acclimated to 25 and 30°C displayed similar growth rates, while fish acclimated to 35°C had very poor growth rates. The SMR and LDOT experiments were performed using intermittent respirometers. Fish acclimated to 25°C had the lowest SMR, followed by 30°C, and finally 35°C. The highest LDOT …

Targeting Bcl-2 Family Proteins In Therapy Induced Senescent Cancer Cell Models, Wade Cook

Theses and Dissertations

Non-small Cell Lung Cancer (NSCLC) originates from numerous different cell types in the lungs and is among the deadliest of cancers. Head and Neck Squamous Cell Carcinomas (HNSCC) are derived from the mucosal membranes of the oral cavity, pharynx, and larynx. Both NSCLC and HNSCC are predominately caused by tobacco smoke inhalation and as such mutations in the tumor suppressor gene TP53 are common. Since similarities exist in the root cause of NSCLC and HNSCC, they may also share similarities in treatment methods. Cisplatin is a platinum-based DNA damaging agent that has been used as a cancer chemotherapy for decades. …

Relationship Of Winter Hair Coat Shedding Ability Of Purebred Angus Dams With Mineral Status, Apparent Forage Digestibility, Uterine Artery Hemodynamics, And Calf Growth And Development, Randy Hunter Burnett

Theses and Dissertations

Winter hair coat shedding ability in southern U.S. cattle production has previously been associated with differences in adjusted birth and weaning weights of calves from dams that shed by June or later (Gray et al., 2011; Cauble et al., 2019). This study was developed to research possible avenues of why these relationships exist with winter hair coat shedding ability. The primary objectives of the following experiments were to: 1) evaluate how mineral status relates to winter hair coat shedding ability, 2) evaluate how winter hair coat shedding ability relates to apparent digestibility of forage, 3) evaluate winter hair coat shedding …

The Art Of Amphibian Conservation: Linking In-Situ And Ex-Situ Populations Of Endangered Species Through Genome Banking, Isabella Joann Burger

Theses and Dissertations

Limited breeding success in captive breeding programs has necessitated the development of assisted reproductive technologies (ART) to preserve and increase genetic variation and population numbers of both captive and wild amphibian groups. ART has been shown to be successful in numerous anuran species, and current studies focus on the application of ART in ex-situ populations. The focus of this project is to show that linking in-situ and ex-situ amphibian populations through sperm cryopreservation, genome banking, and in-vitro fertilization is possible, with the goal of increasing gene diversity throughout groups in order to produce self-sustaining, wild populations in the future. Specific …

Handgrip Strength In Cancer Survivors: 2011-2014 National Health And Nutrition Examination Survey, Ayla Monet Kibler

Theses and Dissertations

Cachexia, or a loss of muscle mass, has been identified as a potential indicator of cancer survival rates. Previous explorations in research have connected muscular strength to muscle loss and cancer survivorship, but nationally generalizable findings are limited. PURPOSE: To investigate handgrip strength in cancer survivors using the National Health and Nutrition Examination Survey (NHANES), a nationally representative sample of US adults. METHODS: The analysis included 368 males and 425 females from the 2011-2014 NHANES dataset. Cancer survivor information (type of cancer and age of diagnosis) was determined by questionnaire. Cancer types were grouped into the following: skin cancers, reproductive …

Effects Of Exercise Intensity And Type On Executive Function In Elementary-Aged Children, Patrick Pommier

Theses and Dissertations

The interactions of exercise on executive functioning (EF) have been studied thoroughly in previous literature, focusing mostly on the interactions of aerobic exercise (AE) intensity and its effects on inhibition and working memory. Although multiple categories of EF have been shown to improve based on aerobic exercise, the foundation of brain development relies within its ability to utilize all three areas of EF simultaneously. Therefore, the purpose of this study was to determine the effects of exercise intensity and type of exercise (cognitive activation) on EF in elementary-aged children. Design: Eighteen children (mean age=10.4, SD=0.5, 61.1% girls) participated in a …

Modeling Individual Muscle Forces In The Lower Extremity During Loaded And Unloaded Hexbar Vertical Jumps, Abigail Salvadore

Theses and Dissertations

Hexagonal barbell (HB) loaded jumps are often used in training to increase lower extremity power. Given the importance of coordinated muscular effort in achieving maximal power output, an understanding of how the lower extremity musculature individually performs during loaded jumps would be advantageous. The purpose of this study is to describe the effect of load on individual muscle forces, muscle torques, and the contribution to the net joint moment (NJM) during the concentric phase of loaded HB jumps.10 male collegiate athletes performed 5 maximal HB jumps at 0%, 20%, 40% and 60% of their HB deadlift 1-repetition maximum. Filtered Ground …

Role Of P53 Deficient Clonogenic Endothelial Cells In The Pathogenesis Of Pulmonary Hypertension, Aneel Roy Bhagwani

Theses and Dissertations

Pulmonary Artery Hypertension (PAH) is a progressive disorder of the small vessels of the pulmonary vasculature. It is characterized by occlusive vascular remodeling leading to right heart failure and ultimately death. PAH pathogenesis involves a multifactorial etiology leading to formation of occlusive lesions in small (preacinary) pulmonary arteries. These lesions contain hyper-proliferative, apoptosis resistant endothelial cells (ECs), smooth muscle cells and fibroblasts. ECs play a major role in the development of PAH. These ECs in vascular lesions are primitive and express progenitor cell markers, including CD117 (c-kit).

We have isolated clonally expanded, c-kit+ ECs from the lungs of Green Fluorescent …

A Novel Review Of Heat Shock Protein 110 Kda: A Basis For Research And Continued Experimentation Through Biochemical Analysis, Crist W. Cuffee

Theses and Dissertations

Heat shock protein 110 kDa, Hsp110, is a distinct cellular protector, different in form and function from Hsp70, a close relative of Hsp110. Functioning primarily as a holdase or in tandem with other molecular chaperones, a review of current accomplishments elucidates the uniqueness of this protein and the continued mysteries that surrounds it. Found only in eukaryotes, Hsp110 has been linked to many diseases, ranging from parasitic infection to neurodegenerative disorders. While still lacking, studies of this protein have provided much in the realm of speculation on the mechanisms behind Hsp110s’ contribution to different pathologies. This review will serve as …

Muscarinic Excitation Of Dopamine Neurons In The Ventral Tegmental Area Via Activation Of A Trpc-Like Cation Conductance, Yu Tzu Chen

Theses and Dissertations

Dopaminergic (DA) neurons in the ventral tegmental area (VTA) play a crucial role in reward and motivational behaviors, including the development of drug addictions. VTA DA neurons receive excitatory cholinergic inputs from the mesopontine tegmentum. Blockage of the M5 muscarinic receptor in DA neurons has been shown to attenuate drug-induced DA release and abuse-related behaviors, but the molecular mechanism is unknown. In this study, experiments were designed to identify the electrophysiological effects of muscarinic agonism in the modulation of action potential kinetics and firing patterns in VTA DA neurons of mice. Pharmacology of the muscarinic receptor-evoked current was also characterized. …

A Computational Model Relating Tissue Oxygen Consumption To Oxygen Delivery In A Krogh Cylinder Model Of Skeletal Muscle, Raghad A. Alqahtani

Theses and Dissertations

Oxygen transport from a capillary to skeletal muscle tissue is a complex process that involves convective and diffusive mechanisms to deliver adequate oxygen to meet tissue metabolic activities. Typically, oxygen uptake in tissue is set by oxygen demand, which is set by metabolic activity. The relationship between the oxygen consumption (VO₂) of an isolated perfused tissue and the rate of delivery of oxygen (QO₂) to the tissue has been a subject of interest to many investigators over the past century. Experiments have shown that there is a critical value of QO₂ below which tissue VO …

Impact Of Physical Activity And Cardiorespiratory Fitness On Vascular Homeostasis In Premenopausal Women Consuming A Westernized High-Fat Meal, Lauren N. Pedersen

Theses and Dissertations

A large percentage of adults in industrialized nations consume a Westernized dietary pattern, which contributes to fasting dyslipidemia and increased risk for atherosclerotic cardiovascular disease. Evidence suggests that a single Westernized meal containing high saturated fat may induce alterations in blood lipids as well as disturb multiple factors regulating peripheral vascular homeostasis, including innate immune cell function, oxidative stress, and vascular function. Conversely, participation in acute and chronic exercise may beneficially impact an individual’s postprandial response. Therefore, this dissertation contains three manuscripts seeking to describe how acute exercise, cardiorespiratory fitness, and physical activity level impact the lipemic and vascular homeostatic …

Reconsidering Undergraduate Anatomy And Physiology Curriculum, Eric Michael Walsh

Theses and Dissertations

In the past 50 years several convergent factors have spurred unprecedented change in anatomy and physiology (A&P) instruction. An explosion of biomedical knowledge, an increased demand for students well-versed in A&P, and decreased resources for the course has provoked a vast array of research. However, most studies employ one of three strategies to enact change: testing the effectiveness of new pedagogies, exploring the utility of new technologies, or documenting learning in new contexts. By comparison, a much smaller body of research focuses on changing the A&P curriculum to improve student learning. To address this gap, the musculoskeletal curriculum for an …

Genetic And Cellular Models For The Study Of Ptk2b And Adam10 In Adaptive Thermogenesis And Metabolism, Jared S. Farrar

Theses and Dissertations

In a cellular model, we demonstrate that the non-receptor protein tyrosine kinase 2 beta (PTK2B) plays a critical role in mouse cultured beige adipocyte differentiation. CRISPR/Cas9-mediated knock-out of Ptk2b results in non-differentiating white adipocytes and differentiated beige adipocytes with significantly reduced thermogenic gene and protein expression, enlarged lipid droplet size, and altered mitochondrial respiration. Together, these data in a cell culture system provide evidence for a role of PTK2B in the differentiation of mouse beige adipocytes.

In the process of developing a new mouse model utilizing the adipocyte selective Adipoq-Cre transgenic mouse, strong genetic linkage between a gene …

Focal Augmentation Of Somatostatin Interneuron Function And Subsequent Circuit Effects In Developmentally Malformed, Epileptogenic Cortex, Nicole Ekanem

Theses and Dissertations

Drug-resistant epilepsy (DRE) is a common clinical sequela of developmental cortical malformations such as polymicrogyria. Unfortunately, much remains unknown about the aberrant GABA-mediated circuit alterations that underlie DRE's onset and persistence in this context. To address this knowledge gap, we utilized the transcranial freeze lesion model in optogenetic mice lines (Somatostatin (SST)-Cre or Parvalbumin (PV)-Cre x floxed channelrhodopsin-2) to dissect features of the SST, PV, and pyramidal neuron microcircuit that are potentially associated with DRE. Investigations took place within developmental microgyria’s known pathological substrate, the adjoined and epileptogenic paramicrogyral region (PMR). As well, microcircuit relationships within the previously unexplored range …

Structural Features Contributing To Methylenedioxypyrovalerone (Mdpv) Analog Inhibition Of The Human Dopamine Transporter, Zachary R. Spires

Theses and Dissertations

Monoamine transporters (MATs) are transmembrane neurotransmitter sodium symporters consisting of the dopamine, norepinephrine, and serotonin transporters: DAT, NET, and SERT, respectively. Following an action potential and neurotransmitter vesicular release, MAT-mediated reuptake of these neurotransmitters in the presynaptic membrane contributes to the termination of monoaminergic neurotransmission. MATs are the targets of many drugs providing therapeutic benefits or taken recreationally. One of these drugs is methylenedioxypyrovalerone (MDPV), an abused synthetic cathinone that is a high potency uptake inhibitor of the human DAT (hDAT). In contrast, MDPV is a low potency uptake inhibitor of the Drosophila melanogaster dopamine transporter (dDAT). The reasons for …

Novel Duel Mtor Inhibitors/Ampk Activators Improve Therapeutic Efficacy Of Doxorubicin And Ameliorate Its Associated Cardiotoxicity In Mice, Sahak Eric Hovsepian

Theses and Dissertations

Triple-negative breast cancer (TNBC) is a particularly aggressive subset of breast cancer that has a molecular expression profile which lacks the estrogen receptor (ER), progesterone receptor (ER), and human epidermal growth factor receptor type 2 (HER2). No targeted treatment options currently exist for TNBC, unlike other types of breast cancer, and therefore survival rates are far lower for these patients. The current treatment for TNBC is systemic anthracycline chemotherapy, typically doxorubicin (DOX), which has great clinical efficacy in increasing survival. However, DOX induces a dose-dependent progressive cardiomyopathy, which can present years and up to decades after the last treatment. This …

An Identification Of Tgf-B'S Role In Ischemic Cholangiopathy, Courtney Chmielewski

Theses and Dissertations

Organ transplants are a necessary intervention for many diseases that result in end stage organ failure. The donation pool cannot match the demands of the transplant list, so expanding the pool to include donation after cardiac death (DCD) is desired. However, there are increased odds of graft failure and ischemic cholangiopathy leading to inferior outcomes when DCD livers are used. Ischemic cholangiopathy consists of multiple diffuse strictures and fibrosis of the bile ducts leading to a loss of epithelialization and fibrosis. The cellular mechanism is proposed to be epithelial-to-mesenchymal transition (EMT). TGF-�� is seen as a key initiator of EMT, …