Physiology Commons^™

Sarcopenia And The Importance Of Resistance Training And Protein-Rich Diets For Prevention Of Muscle Loss In Older Adults: A Literature Review And Informational Pamphlet, Madison Steele

Honors Theses

The growing prevalence of sarcopenia among older adults as well as youths has prompted further research aimed at developing effective preventative measures and treatment plans. Sarcopenia is often thought to occur solely due to aging, but several other factors contribute to progressive muscle loss. Previous research studies have found an effect of physical activity and dietary protein levels on the risk of developing sarcopenia as well as its onset age. This study aims to review existing knowledge in the field to compile an extensive list of sarcopenia causes and methods of prevention. The main causes identified in this review are …

Effects Of Aging On Post-Exercise Oxygen Consumption: A Preliminary Study, Steven Klepacz

Health, Exercise, and Sports Sciences ETDs

Aging and excess post-exercise oxygen consumption (EPOC) is something that has not been adequately studied. Studies looking at EPOC mostly are compiled of young college age individuals. Aging in humans is a topic of much research with still more to be learned. The study in this paper attempts to answer the question of whether age affects EPOC when performing a certain exercise. Preliminary findings based on general age-related research suggest the possibility. This thesis will explore the current research related to age and EPOC, analyze the findings, and discuss additional avenues for future research.

Sex Differences And Physical Activity Status On The Hamstring: Quadriceps Ratio, Activities Of Daily Living, And Functional Movement In Older Adults, Brittany N. Followay, Haley A. Reierson, Emily M. Rigden

International Journal of Exercise Science

International Journal of Exercise Science 16(4): 1228-1243, 2023. The study aimed to investigate sex differences and the effect of physical activity on the hamstring:quadriceps (H:Q) ratio, activities of daily living (ADLs), and the Functional Movement Screen (FMS) in older adults. Thirty older adults (72.56 + 6.22) completed maximal voluntary isometric contractions (MVICs), ADLs, the FMS, and three closed-kinetic chain exercises (CKCs); front lunge (FL), side lunge (SL), and bilateral squat (BLSQ). Electromyography was recorded from the biceps femoris (BF) and vastus lateralis (VL) on the dominant and non-dominant limbs during the MVICs …

Impacts Of Dietary Restriction On A Drosophila Model Of Werner Syndrome, Eileen Sember

College of Arts & Sciences Senior Honors Theses

Werner syndrome (WS) is an autosomal recessive disorder that results in premature aging and occurs in 1 in 1,000,0000 to 1 in 10,000,000 people. In humans, WS is the result of mutations that render the WRN gene, that contains a helicase and an exonuclease domain, non-functional. Currently, there is no cure for WS in humans, making dietary and lifestyle interventions attractive for increasing the quality and longevity of lives. Diet restriction (DR) has been shown to extend the lifespan of several model organisms, including Drosophila melanogaster, making it a strong candidate for WS treatment. In this thesis, mutant flies …

Atrial Fibrillation In Aging: Mechanisms And Potential Therapeutics, Mengmeng Chang

USF Tampa Graduate Theses and Dissertations

Atrial fibrillation (AF) is one of the most common cardiac arrhythmias seen in the clinics, and currently available antiarrhythmic pharmacotherapies in AF are not very effective. Although AF has been recognized as an aging-mediated disease, our understanding of the electrophysiological pathways that link aging and AF remain incomplete, which limits breakthroughs in the development of novel antiarrhythmic treatments for this disease. Studies have shown that aging increases the generation of reactive oxygen species (ROS) in the heart, and high levels of ROS have been associated with development of AF in animals and patients. Additionally, in some forms of AF, the …

Functional Benefits Of Hard Martial Arts For Older Adults: A Scoping Review, Ian Miller, Mike Climstein, Luke Del Vecchio

International Journal of Exercise Science

International Journal of Exercise Science 15(3): 1430-1443, 2022. The present scoping review focused upon the functional benefits of hard martial arts training for older adults. The AXIS Critical Appraisal Tool was used to appraise all studies included in the review. A systematic literature search identified 265 papers via electronic database and ten papers from additional sources. Of these, six studies featuring 240 participants were included in the final scoping review. Of the included studies, three were randomized control trials and three were comparative studies with concurrent controls. The available studies outlined improvements in functional fitness parameters for older adults participating …

Acute Effects Of Velocity-Based Resistance Training On The Physical Functional Performance Of Older Adults, Suzy S. Pinto, Ewertton S. Bezerra, Keuly G. Silva, Rodrigo Ramirez-Campillo, Abderaouf B. Abderrahman

International Journal of Exercise Science

International Journal of Exercise Science 15(3): 399-413, 2022. The aim of this study was to analyse the acute effects of velocity-based resistance training on the physical and functional performance of older adults. Twenty participants (70.4 ± 7.4 years) performed the deadlift exercise, in two different resistance training protocols. The moderate-velocity protocol (MV) predicted maximum loads so that the movement velocity during the concentric phase remained in the range of 0.5 to 0.7 m/s and the high-velocity protocol (HV) predicted maximum loads so that the movement velocity remained between 0.8 and 1.0 m/s. The jump height (cm), handgrip strength (kg), and …

Acute Inflammatory Profiles Differ With Sex And Age After Spinal Cord Injury, Andrew N. Stewart, John L. Lowe, Ethan P. Glaser, Caitlin A. Mott, Ryan K. Shahidehpour, Katelyn E. Mcfarlane, William M. Bailey, Bei Zhang, John C. Gensel

Physiology Faculty Publications

Background

Sex and age are emerging as influential variables that affect spinal cord injury (SCI) recovery. Despite a changing demographic towards older age at the time of SCI, the effects of sex or age on inflammation remain to be elucidated. This study determined the sex- and age-dependency of the innate immune response acutely after SCI.

Methods

Male and female mice of ages 4- and 14-month-old received T9 contusion SCI and the proportion of microglia, monocyte-derived macrophages (MDM), and neutrophils surrounding the lesion were determined at 3- and 7-day post-injury (DPI) using flow cytometry. Cell counts of microglia and MDMs were …

Decreased Activity Of Phosphofructokinase-1 In Flight Muscle Cells Of Hawk Moth Manduca Sexta With Age, Owen G. Alvine

Honors Thesis

The phosphofructokinase-1 (PFK-1) enzyme is important for the catalyzation and regulation of glycolysis, especially in muscle. Investigating age-related changes in PFK-1 activity will provide insights into the metabolism shifts in muscle cells of our muscle aging model. The hawk moth, Manduca sexta, was chosen as the model organism because of its unique endothermic, synchronous flight muscles that are more analogous to vertebrates than invertebrate species. We hypothesized that PFK-1 activity will increase in muscle cells of aged moths due to dysregulation of the mitochondria. This was predicted to change the method of energy production, by hindering oxidative phosphorylation, making glycolysis …

Postprandial Exhaled Nitric Oxide Responses In Older And Younger Adults With And Without Acute Exercise, Molly Miller

Masters Theses, 2020-current

A single high fat meal (HFM) increases airway inflammation in young, healthy individuals. Additionally, aging increases airway resistance and inflammation, though the airway inflammatory response to a HFM has not been investigated. Exercise is a natural anti-inflammatory, but has yet to be administered with a HFM as a method to study postprandial airway inflammation in older adults. Purpose: To investigate whether older individuals have greater postprandial airway inflammation compared to younger counterparts, and to explore whether exercise may modify the postprandial airway response in older adults. Methods: 12 younger adults (23.3±3.9 years; 5 M/7 F) and 12 older adults (67.7±6 …

Self-Selected Maximum But Not Jogging Speed Decreases With Age In Male And Female Runners, Heather Hamilton, Rumit Singh Kakar

College of Health Sciences Posters

Introduction: Running velocity decreases with age, likely resulting from physiological and musculoskeletal changes associated with aging. Females experience a more rapid decline in physical performance during middle age than males, therefore it is important to consider separate-sex analyses when studying running biomechanics and running-related injury. The purpose of this study was to determine the relationship between age and different running speeds separately for female and male runners.

Methods: Male (n=33) and female (n=46) runners (ages 18–65 years) ran at their self-selected JOG (long distance) and MAX (maximal running) pace on a treadmill. Running velocity was recorded. Simple linear regression was …

Inhibition Of Grk2, But Not Hsp90 Reduces Mitochondrial Superoxide And Improves Vasodilation Capacity Of Coronary Arterioles From Aged Female Rats, Michaela N Dukes, Evan P. Tracy B.S., Amanda Jo Leblanc Ph.D.

Undergraduate Arts and Research Showcase

Introduction: In post-menopausal women, small coronary microvessel dysfunction is the predominant heart disease presentation.There is currently no single treatment to tackle the multifactorial etiology of micro vessel dysfunction including oxidative stress, hyper constricted state, endothelial dysfunction, and blunted beta-adrenergic function. In the heart, G protein-coupled receptors (GPCRs), such as the b-adrenergic receptor (bADR) investigated in this experiment, are active in mediating vessel contractility vasodilation to facilitate blood flow. The presence of GPCR kinase 2 (GRK2), which inhibit bADRs, increase in old age and may mediate blunted capacity to vasodilate. Furthermore, GRK2 can be translocated to the mitochondria via heat shock …

Insights On Ten Weeks Of Classical Ballet Training And Postural Stability In Older Adults, Hannah Weighart, Sarah Dipasquale

International Journal of Exercise Science

International Journal of Exercise Science 13(1): 101-112, 2020. Older adults show an increased risk of falling as they age, but dance interventions of various genres have been shown to improve postural stability in this population. The purpose of this study was to investigate the effects of a ten-week beginning ballet intervention on postural stability for older adults. Eleven participants enrolled in the Dance Group (DG; 73.3 ± 10.6 years) while six enrolled in the Control Group (CG; 69.5 ± 11.9 years) via convenience sample. Following the intervention, no significant differences were seen within the DG from pre- to post-testing or …

Divergence In Neuronal Calcium Dysregulation In Brain Aging And Animal Models Of Ad, Adam Ghoweri

Theses and Dissertations--Pharmacology and Nutritional Sciences

Neuronal calcium dysregulation first garnered attention during the mid-1980’s as a key factor in brain aging, which led to the formulation of the Ca²⁺ hypothesis of brain aging and dementia. Indeed, many Ca²⁺-dependent cellular processes that change with age, including an increase in the afterhyperpolarization, a decrease in long-term potentiation, an increased susceptibility to long-term depression, and a reduction in short-term synaptic plasticity, have been identified. It was later determined that increased intracellular Ca²⁺ with age was due to increased Ca²⁺ channel density, elevated release from intracellular Ca²⁺ stores, and decreased Ca²⁺ buffering …

Post-Acquisition Processing Confounds In Brain Volumetric Quantification Of White Matter Hyperintensities, Ahmed A. Bahrani, Omar M. Al-Janabi, Erin L. Abner, Shoshana H. Bardach, Richard J. Kryscio, Donna M. Wilcock, Charles D. Smith, Gregory A. Jicha

Neurology Faculty Publications

BACKGROUND: Disparate research sites using identical or near-identical magnetic resonance imaging (MRI) acquisition techniques often produce results that demonstrate significant variability regarding volumetric quantification of white matter hyperintensities (WMH) in the aging population. The sources of such variability have not previously been fully explored.

NEW METHOD: 3D FLAIR sequences from a group of randomly selected aged subjects were analyzed to identify sources-of-variability in post-acquisition processing that can be problematic when comparing WMH volumetric data across disparate sites. The methods developed focused on standardizing post-acquisition protocol processing methods to develop a protocol with less than 0.5% inter-rater variance.

RESULTS: A series …

The Association Of Handgrip Strength And Mortality: What Does It Tell Us And What Can We Do With It?, Samuel L. Buckner, Scott J. Dankel, Zachary W. Bell, Takashi Abe, Jeremy P. Loenneke

Faculty and Student Publications

© Copyright 2019, Mary Ann Liebert, Inc., publishers 2019. The relationship between grip strength and mortality is often used to underscore the importance of resistance exercise in physical activity guidelines. However, grip strength does not appear to appreciably change following traditional resistance training. Thus, grip strength could be considered reflective of strength independent of resistance exercise. If true, grip strength is not necessarily informing us of the importance of resistance exercise as an adult, but potentially highlighting inherent differences between individuals who are stronger at "baseline" compared to their weaker counterpart. The purpose of this article is to discuss: (1) …

Effects Of Life-Long Wheel Running Behavior On Plantar Flexor Contractile Properties, Alexander Nicholas Beechko

Electronic Theses, Projects, and Dissertations

Aging in skeletal muscle is characterized by a loss in muscular performance. This is in part related to the direct loss of muscle mass due to senescence, known as sarcopenia. With age, skeletal muscles lose force production, contractile speed, and power production. The force velocity relationship of muscle is a product of force production and contraction speed, both of which decline with age; however, the mechanisms and trajectory of this decline are not well understood. Exercise has positive effects on muscle, and thus may assist in maintaining performance in old age. However, few long-term studies have been performed to examine …

Mechanics Of Phenotypic Aging Trajectories In C. Elegans And Humans, William Zhang

Arts & Sciences Electronic Theses and Dissertations

Overall, my dissertation integrates longitudinal measurements of physiology to investigate the aging process. In the first half, I examine the surprising and largely unexplained degree of variation in lifespan within even homogeneous populations. I sought to understand how physiological aging differs between long- and short-lived individuals within a population of genetically identical C. elegans reared in a homogeneous environment. Using a novel culture apparatus, I longitudinally monitored aspects of aging physiology across a large population of isolated individuals. Aggregating several measures into an overall estimate of senescence, I find that long- and short-lived individuals start adulthood on an equal physiological …

Similar Effects Of 24 Weeks Of Resistance Training Performed With Different Frequencies On Muscle Strength, Muscle Mass, And Muscle Quality In Older Women, Fábio Luiz Cheche Pina, João Pedro Nunes, Matheus Amarante Nascimento, Alex Silva Ribeiro, Jerry L. Mayhew, Edilson Serpeloni Cyrino

International Journal of Exercise Science

International Journal of Exercise Science 12(6): 623-635, 2019. The purpose of this study was to analyze the effects of 24 weeks of resistance training (RT) performed 2 vs. 3 times per week on muscle strength, muscle mass, and muscle quality in older women. Thirty-nine older women (≥ 60 years old) were randomly assigned to two groups according to RT frequency (G2x = two sessions per week, n=19; and G3x = three sessions per week, n=20) and were submitted to 24 weeks of whole-body RT, divided into two stages of 12 weeks. In the first stage, participants performed 1 set of …

The Role Of Sirt1 In Skeletal Muscle Function And Repair Of Older Mice, Matthew John Myers

Graduate Theses, Dissertations, and Problem Reports

Human skeletal muscle is a highly metabolic tissue necessary for mobility and coordination. Responsible for approximately one-fifth of the resting human metabolism, skeletal muscle is also an important regulator of metabolites like glucose and contributes to the regulation of body temperature. Although there is a gradual decline in muscle mass associated with aging, a certain percentage of the population suffer from severe muscle mass and strength deterioration, classified as sarcopenia (5-13% for people aged 60-70 years old, 11-50% for those 80 or older). Sarcopenia is linked to increased morbidity and mortality rates in the elderly population, while annual healthcare costs …

Overexpression Of Cyb5r3 And Nqo1, Two Nad+-Producing Enzymes, Mimics Aspects Of Caloric Restriction, Alberto Diaz-Ruiz, Michael Lanasa, Joseph Garcia, Hector Mora, Frances Fan, Alejandro Martin-Montalvo, Andrea Di Francesco, Miguel Calvo-Rubio, Andrea Salvador-Pascual, Miguel A. Aon, Kenneth W. Fishbein, Kevin J. Pearson, Jose Manuel Villalba, Placido Navas, Michel Bernier, Rafael De Cabo

Pharmacology and Nutritional Sciences Faculty Publications

Calorie restriction (CR) is one of the most robust means to improve health and survival in model organisms. CR imposes a metabolic program that leads to increased stress resistance and delayed onset of chronic diseases, including cancer. In rodents, CR induces the upregulation of two NADH‐dehydrogenases, namely NAD(P)H:quinone oxidoreductase 1 (Nqo1) and cytochrome b₅ reductase 3 (Cyb5r3), which provide electrons for energy metabolism. It has been proposed that this upregulation may be responsible for some of the beneficial effects of CR, and defects in their activity are linked to aging and several age‐associated diseases. However, …

Body Composition, Muscular Performance, And Free Testosterone Following 12-Weeks Of Protein Supplementation And Resistance Training In Men Aged 35-55, Matthew Steven Stone

Graduate Theses and Dissertations

PURPOSE: The purpose of this study was to investigate the effects of 12 weeks of protein supplementation and Autoregulatory Progressive Resistance Exercise (APRE) training on measures of body composition, muscular performance, and free testosterone in middle-aged men. METHODS: Untrained males (n = 35; 43.3 ± 6.6 years) participated in this study. Lean mass (LM) and fat mass (FM) were measured via Dual Energy X-ray Absorptiometry (DEXA), while strength was measured utilizing one repetition maximum (1RM), endurance measured using maximum repetitions completed (REPS) at 75% 1RM for the bench and leg press, and free testosterone was measured using changes in serum …

Investigating The Role Of Pannexin 3 In Intervertebral Disc Health And Disease, Meaghan E. Serjeant

Electronic Thesis and Dissertation Repository

Pannexin 3 (Panx3) is a mechanosensitive, channel-forming glycoprotein implicated in the progression of post-traumatic osteoarthritis. Despite evidence for Panx3 expression in intervertebral discs (IVDs), its function here remained unknown. Using Panx3^-/- mice, this study investigated the role of Panx3 in age-associated and injury-induced models of IVD degeneration. While loss of Panx3 did not significantly impact the progression of age-associated IVD degeneration, it was associated with a protective phenotype in an injury model of IVD degeneration, specifically in the annulus fibrosus (AF). Following IVD injury, WT mice showed an accumulation of enlarged cells that appeared to contribute to disrupted AF …

Relative Handgrip Strength As A Simple Tool To Evaluate Impaired Heart Rate Recovery And A Low Chronotropic Index In Obese Older Women, Cristiane Rocha Da Silva, Dahan Da Cunha Nascimento, Ivo Sousa Neto, Ramires Tibana, Samuel Oliveira, Renato Valduga, Nayara Santos, Bruno Saraiva, Jonato Prestes, Jeffrey Willardson, Guilherme Borges Pereira

International Journal of Exercise Science

International Journal of Exercise Science 11(2): 844-855, 2018. The aim of the present study was to compare differences in heart rate response following a treadmill exercise test in elderly obese women categorized into groups based on relative handgrip strength. Eighty-eight obese elderly women who were between the ages of 60 and 87 participated in the study and were categorized and enrolled to one of two groups based on lower (< 1.51 m²) or higher (≥ 1.51 m²) relative handgrip strength, respectively. The heart rate recovery in the first and second minutes following the treadmill exercise test and the chronotropic index were compared between groups. The higher relative handgrip strength group presented a significantly higher peak heart rate during exercise and a quicker heart rate recovery following exercise versus the lower relative handgrip strength group (pp=0.059) toward a significantly greater chronotropic index in the higher versus the lower relative handgrip strength group. In conclusion, elderly women with greater relative handgrip strength also demonstrated a …

Serum Tumor Necrosis Factor-Alpha Associates With Myocardial Oxygen Demand And Exercise Tolerance In Postmenopausal Women, Stephen J. Carter, David R. Bryan, William H. Neumeier, Stephen P. Glasser, Gary R. Hunter

International Journal of Exercise Science

International Journal of Exercise Science 11(2): 42-54, 2018. The functional implications of serum tumor necrosis factor-alpha (TNF-a), a marker of oxidative stress, on hemodynamic parameters at rest and during physical exertion are unclear. The aims of this investigation were to examine the independent associations of TNF-a on myocardial oxygen demand at rest and during submaximal exercise, while also evaluating the association of TNF-a on exercise tolerance. Forty, postmenopausal women, provided blood samples and completed a modified-Balke protocol to measure maximal oxygen uptake (VO_2max). Large artery compliance was measured by pulse contour analyses while rate-pressure product (RPP), an index …

Examining Effects Of Arousal And Valence Across The Adult Lifespan In An Emotional Stroop Task, Samantha E. Tuft

ETD Archive

As age increases, there is evidence that people tend to pay less attention to negative information, pay more attention to positive information, or both. There are many theoretical accounts that attempt to explain this positivity bias. In the current study, I examined positivity effects across the adult lifespan by evaluating competing predictions of two theories: Socioemotional Selectivity Theory, which is based in motivation, and Dynamic Integration Theory, which is based in capacity. Computer mouse tracking was used to examine effects across levels of Valence (negative, neutral, and positive) and Arousal (low, medium, and high) in an emotional Stroop task. Participants …

Dysregulation Of Daf-16/Foxo3a-Mediated Stress Responses Accelerates T Oxidative Dna Damage Induced Aging, Aditi U. Gurkar, Andria R. Robinson, Yuxiang Cui, Xuesen Li, Shailaja K. Allani, Amanda Webster, Mariya Muravia, Mohammad Fallahi, Herbert Weissbach, Paul D. Robbins, Yinsheng Wang, Eric E. Kelley, Claudette M. St. Croix, Laura J. Niedernhofer, Matthew S. Gill

Faculty & Staff Scholarship

DNA damage is presumed to be one type of stochastic macromolecular damage that contributes to aging, yet little is known about the precise mechanism by which DNA damage drives aging. Here, we attempt to address this gap in knowledge using DNA repair-deficient C. elegans and mice. ERCC1-XPF is a nuclear endonuclease required for genomic stability and loss of ERCC1 in humans and mice accelerates the incidence of age-related pathologies. Like mice, ercc-1 worms are UV sensitive, shorter lived, display premature functional decline and they accumulate spontaneous oxidative DNA lesions (cyclopurines) more rapidly than wild-type worms. We found that ercc-1 worms …

Micrornas, Heart Failure, And Aging: Potential Interactions With Skeletal Muscle, Kevin A. Murach, John J. Mccarthy

Center for Muscle Biology Faculty Publications

MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by targeting mRNAs for degradation or translational repression. MiRNAs can be expressed tissue specifically and are altered in response to various physiological conditions. It has recently been shown that miRNAs are released into the circulation, potentially for the purpose of communicating with distant tissues. This manuscript discusses miRNA alterations in cardiac muscle and the circulation during heart failure, a prevalent and costly public health issue. A potential mechanism for how skeletal muscle maladaptations during heart failure could be mediated by myocardium-derived miRNAs released to the circulation is presented. An overview …

Moderate-Load Muscular Endurance Strength Training Did Not Improve Peak Power Or Functional Capacity In Older Men And Women, Simon J. Walker, Greg G. Haff, Keijo K. Häkkinen, Robert U. Newton

Research outputs 2014 to 2021

The present study determined the effects of muscular endurance strength training on maximum strength and power, functional capacity, muscle activation and hypertrophy in older men and women. Eighty-one men and women acted as an intervention group while 22 acted as non-training controls (age range 64-75 y). Intervention training included super-sets (i.e., paired exercises, immediately performing the second exercises following completion of the first) with short rest intervals (30-60 s between sets) at an intensity of 50-60% one-repetition maximum (1-RM) for 15-20 repetitions. Concentric leg press actions measured maximum strength (1-RM) and concentric peak power. Functional capacity was assessed by maximum …

Extracellular Matrix Remodeling And The Inflammatory Response During Skeletal Muscle Regeneration In Sarcopenic Obese Mice, Lemuel Arthur Brown

Graduate Theses and Dissertations

AIM: Sarocpenic obesity is a national concern within the United States because this metabolic syndrome is tied with reduced mobility and quality of life. Both obesity and aging are associated with insulin-resistance, chronic low-grade inflammation and muscle weakness. Skeletal muscle regeneration is a process that involves the coordinated effort of myogenic regulatory factors (MRFs), inflammatory signaling, and extracellular matrix (ECM) remodeling for optimal regeneration. It has been demonstrated that obesity and aging have a reduction in muscle regeneration. It has not been examined if sarcopenic obesity will further reduce muscle mass and the regenerative process. The purpose of this study …