Physiology Commons^™

Effects Of Target-Controlled Infusion Of High-Dose Naloxone On Pain And Hyperalgesia In A Human Thermal Injury Model: A Study Protocol, Anders D. Springborg, Elisabeth K. Jensen, Bradley K. Taylor, Mads U. Werner

Physiology Faculty Publications

Mu-opioid-receptor antagonists have been extensively studied in experimental research as pharmacological tools uncovering mechanisms of pain modulation by the endogenous opioid system. In rodents, administration of high doses of mu-opioid-receptor antagonists after the resolution of an inflammatory injury has demonstrated reinstatement of nociceptive hypersensitivity indicating unmasking of latent sensitization. In a recent human study, pain hypersensitivity assessed as secondary hyperalgesia area (SHA), was reinstated 7 days after a mild thermal injury, in 4 out of 12 subjects after a naloxone infusion.

The aims of the present study are first, to replicate our previous findings in a larger-sized study; second, to …

Regulation Of Microvascular Flow And Metabolism: An Overview, Michelle A. Keske, Renee M. Dwyer, Ryan D. Russell, Sarah J. Blackwood, Aascha A. Brown, Donghua Hu, Dino Premilovac, Stephen M. Richards, Stephen Rattigan

Health & Human Performance Faculty Publications

Skeletal muscle is an important site for insulin to regulate blood glucose levels. It is estimated that skeletal muscle is responsible for ~80% of insulin-mediated glucose disposal in the post-prandial period. The classical action of insulin to increase muscle glucose uptake involves insulin binding to insulin receptors on myocytes to stimulate glucose transporter 4 (GLUT 4) translocation to the cell surface membrane, enhancing glucose uptake. However, an additional role of insulin that is often under-appreciated is its action to increase muscle perfusion thereby improving insulin and glucose delivery to myocytes. Either of these responses (myocyte and/or vascular) may be impaired …

Cardiovascular Regulation By Kvβ1.1 Subunit, Jared Tur

USF Tampa Graduate Theses and Dissertations

Heterologous expression systems such as COS-7 cells have demonstrated the profound effects of KCNAB1-3 or Kvβ1-3 proteins on voltage gated potassium channels (Kv) channels. Indeed, in the presence of these β-subunits transiently expressed Kv channels are often modulated in multiple ways. Kv channel membrane expression is often increased in the presence of β-subunits. In addition, non-inactivating Kv currents suddenly become fast-inactivating and fast-inactivating channels become even faster. While much research has demonstrated the profound effects the β-subunits in particular the Kvβ1 subunit have on transiently expressed Kv currents little to date is known of the physiological role it may play. …

Human Anatomy & Physiology Society ( Haps ) Southern Regional Meeting 2016, Cheryl Purvis

Faculty Proceedings, Presentations, Speeches and Lectures

No abstract provided.

Unusual Presentation Of A Young Man With Disseminated Tuberculosis And Right Ventricular Mass, Shahrukh Hashmani, Fateh Ali Tipoo Sultan, Muhammad Qamar Masood

Section of Cardiology

We report a 22-year-old man who presented to the emergency department with worsening shortness of breath and chronic fever for 2 months. Physical examination was unremarkable except for raised jugular venous pressure and palpable liver. Echocardiogram showed a large right ventricular mass causing obstruction at tricuspid valve. A subsequent chest CT scan confirmed the presence of a large mass in the right ventricle. There were multiple enlarged lymph nodes and consolidation in the right upper lobe. Diagnosis of disseminated tuberculosis(TB) was made and later confirmed by histopathology of lymph node biopsy along with positive sputum culture for acid-fast bacilli. Remarkable …

Maintenance Of The Ph Gradient In The Gastric Mucus Layer., Owen Lewis

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

A 2-D Compartmental Model For Multi-Capillary Supply, Liang Sun, Junkoo Park, Alessandra L. Barrera

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Understanding Adipocyte Dynamics Through Mathematical Modeling, Katrina Johnson, Fred Adler

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Mitochondrial Superoxide Signaling Contributes To Norepinephrine-Mediated T-Lymphocyte Cytokine Profiles., Adam J. Case, Colton T. Roessner, Jun Tian, Matthew C. Zimmerman

Journal Articles: Cellular & Integrative Physiology

Norepinephrine (NE) produces multifaceted regulatory patterns in T-lymphocytes. Recently, we have shown that NE utilizes redox signaling as evidenced by increased superoxide (O2●-) causally linked to the observed changes in these cells; however, the source of this reactive oxygen species (ROS) remains elusive. Herein, we hypothesized that the source of increased O2●- in NE-stimulated T-lymphocytes is due to disruption of mitochondrial bioenergetics. To address this hypothesis, we utilized purified mouse splenic CD4+ and CD8+ T-lymphocytes stimulated with NE and assessed O2●- levels, mitochondrial metabolism, cellular proliferation, and cytokine profiles. We demonstrate that the increase in O2●- levels in response to …

Effect Of Music Tempo On Attentional Focus And Perceived Exertion During Selected Self-Paced Walking, Aldo Coelho Silva, Sandro Dos Santos Ferreira, Sergio Gregorio Da Silva

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 536-544, 2016. This study investigated the influence of music on the rating of perceived exertion (RPE) and attentional focus during walking at a self-selected pace. Fifteen overweight and obese women volunteered to participate in the study. They underwent four sessions: the first for incremental maximal test and anthropometric measurement followed by three experimental sessions. After the first session, they were exposed to three 30-minute walking sessions at a self-selected pace in a counterbalanced order: fast-tempo music (FT), medium-tempo music (MT) and no-music control (NM). Borg’s RPE Scale and an Attentional Focus Questionnaire were used …

Effect Of And Acute Bout Of Kettlebell Exercise On Glucose Tolerance In Sedentary Men: A Preliminary Study, Samantha L. Greenwald, Edward Seger, David Nichols, Andrew Ray, Todd C. Rideout, Luc E. Gosselin

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 524-535, 2016. Impaired glucose tolerance can have significant health consequences. The purposes of this preliminary study were to examine whether a single session of kettlebell exercise improves acute post-exercise glucose tolerance in sedentary individuals, and whether it was as effective as high-intensity interval running. Six sedentary male subjects underwent a two-hour oral glucose tolerance test following three different conditions: 1) control (no exercise); 2) kettlebell exercise (2 sets of 7 exercises, 15 repetitions per exercise with 30 seconds rest between each exercise); or 3) high-intensity interval running (10 one-minute intervals at a workload corresponding …

Sex Differences In Time Perception During Self-Paced Running, Nicholas J. Hanson, Janet Buckworth

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 514-523, 2016. Time perception during exercise may be affected by chosen intensity, and may also affect enjoyment of exercise and subsequent long-term adherence. However, little is known about how individuals perceive the passage of time during exercise, or if factors such as sex are influential. The purpose of this study was to determine if there are sex related differences in perception of time during a bout of exercise in experienced runners. Twenty-two recreational runners (11 men, 11 women) participated in a bout of treadmill running where they were allowed to select their intensity. Sixty …

Acute Effects Of Walking With Nordic Poles In Persons With Mild To Moderate Low-Back Pain, Landon P. Revord, Karen V. Lomond, Peter V. Loubert, Roger L. Hammer

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 507-513, 2016. Regular walking with or without Nordic poles is effective over time at reducing discomfort in individuals with chronic low back pain (LBP). Nordic pole use increases balance and stability, distributes weight through the arms and torso, and decreases loading of the spine and lower limbs. The purpose of this study was to determine if Nordic poles would reduce perceived acute discomfort while self-paced walking in individuals with LBP. We also examined whether walking with or without poles increased heart rate (HR) and ratings of perceived exertion (RPE) or speed of movement. Subjects …

How Feedback And Goal-Setting Impact Children’S Recess Physical Activity, Ryann Koufoudakis, Heather Erwin, Aaron Beighle, Michelle L. Thornton

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 497-506, 2016. In recent years, schools desire to promote physical activity (PA) for their students but are restricted due to resources being expended in other areas of their curriculum, including standardized testing preparation. Recess/lunch periods have potential to contribute important amounts of PA to youth’s overall levels. Interventions to maximize PA during recess are warranted. The purpose of this study was to demonstrate the impact of feedback and goal-setting on students’ PA during recess. A sample of 136 (67 females, 69 males) 4^th and 5^th grade students in the Southeast United States …

The Acute Effect Of Walking On Ultrasound Measurements From The Achilles Insight Ultrasonometer In College-Aged Individuals, Kristin J. Heumann, Jacob Cimolino, Jeremy R. Hawkins, Robert W. Pettitt, Steven Ross Murray

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 491-496, 2016. The Achilles InSight bone ultrasonometer is a portable ultrasound device for quantitatively measuring bone composition both safely and inexpensively via the calcaneus. The effect of acute, brisk walking as a possible source of error on the reliability of quantitative ultrasound (QUS) measurements was investigated. Forty-seven participants (17 women, 30 men; age M ± SD = 20.44 ± 1.16) had their calcaneus measured with the Achilles InSight both before and after a 15-min bout of walking at (5.63 km·h-1 (3.5 mph). The Achilles InSight was deemed reliable via a test-and-retest protocol (ICC α …

Carotid Artery Imt, Blood Pressure, And Cardiovascular Risk Factors In Males And Females, Christina M. Cromwell, Kristin R. Aichele, Joyann E. Oakman, Michael P. Neal, Jessica M. Lenzo, Avery N. Perez, Naomi L. Bye, Erica L. Santaniello, Jessica A. Hill, Rachel C. Evans, Karla A. Thiele, Lauren N. Chavis, Allyson K. Getty, Tia R. Wisdo, Deborah L. Feairheller, Phd

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 482-490, 2016. Previous studies have investigated carotid artery intima-media thickness (IMT) and blood pressure and found a direct correlation between the two. It is known that adult females have better cardiovascular health than males until a certain stage of life, yet limited research has examined gender differences in vascular function. Thus, the purpose of this study was to investigate vascular structure and function, blood pressure, and blood glucose/cholesterol levels in relation to gender differences in young healthy adults. On three separate days, 44 adults (26.30 ±11.9yrs; 24M, 20F) completed a carotid IMT ultrasound, a …

Effects Of A Nighttime Multi-Ingredient Supplement On Recovery From A Damaging Exercise Protocol, Parker Hyde, Ciaran Fairman, Nicholas A. Coker, Keagan Kiely, Mary Beth Yarbrough, Charles Purser, Kristina L. Kendall

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 471-481, 2016. The purpose of this study was to determine the efficacy of a nighttime multi-ingredient supplement on noninvasive markers of recovery in resistance trained and untrained individuals. Forty-nine participants, both trained (n=25) and untrained (n=24) completed the randomized, double blind, placebo-controlled study. Trained participants were randomly divided into supplement (n=12) and placebo (n=13) groups. Untrained participants were randomly divided into supplement (n=14) and placebo (n=10) groups. Two, 2 (supplement group) x 2 (training status) x 5 (time points) repeated measures analysis of variance (ANOVA) were utilized to determine if an interaction for supplement …

The Effect Of A Moderately-Low And High Carbohydrate Intake On Crossfit Performance, Kurt A. Escobar, Jacobo Morales, Trisha A. Vandusseldorp

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 460-470, 2016. CrossFit is a metabolically demanding strength and conditioning method which performance may benefit from a carbohydrate (CHO)-rich diet. This study investigated the effect of three consecutive days of high CHO intake on CrossFit performance and corresponding metabolically -related variables in strength trained individuals. Eighteen subjects with a CHO intake of <6 g/kg/day were randomly assigned into a CHO (n = 9) or control (C) group (n =9) and underwent a 9-day training protocol. During days 1, 5, and 9, performance was measured as repetitions completed during a 12 minute CrossFit workout. Oxygen consumption (VO2), respiratory exchange ratio (RER), and blood lactate (BL) were also measured. Days 6-8, the CHO group increased CHO intake from <6 g/kg/day to 6-8 g/kg/day; the C group maintained their current intake of <6 g/kg/day. On days 6 and 7 both groups performed CrossFit workouts followed by a day of rest prior to day 9. There was a significant increase in repetitions completed in both groups in day 9 (vs. means score of day 1 + 5) (p = 0.002), but no differences between C and CHO groups (p = 0.111). However, the CHO group displayed a 15.2 repetition increase (+10.9%) in day 9, compared to 5.7 (+4.2%) by the C group. VO2, RER, and BL were not influenced by the experimental intervention. Our results suggest that the CrossFit-embraced practice of moderately-low CHO diets may be adequate in CHO during short periods of training, however, given the noted trend, extended training periods may be effected.

Physical Fitness And Self-Image: An Evaluation Of The Exercise Self-Schema Questionnaire Using Direct Measures Of Physical Fitness, Jafra D. Thomas, J. Mark Vanness, Bradley J. Cardinal

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 445-459, 2016. The purpose of this study was to perform a construct validity assessment of Kendzierski’s exercise self-schema theory questionnaire using objective measures of health-related physical fitness. This study tested the hypothesis that individuals with an exercise self-schema would possess significantly greater physical fitness than those who did not across three domains of health-related physical fitness: Body composition, cardiovascular fitness, and upper-body muscular endurance. Undergraduate student participants from one private university on the west coast of the United States completed informed consent forms and the exercise self-schema questionnaire within a classroom setting or at …

Eight Weeks Of Kettlebell Swing Training Does Not Improve Sprint Performance In Recreationally Active Females, Michael E. Holmstrup Ph.D., Brock T. Jensen Ph.D., William S. Evans, Emily C. Marshall

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 437-444, 2016. The kettlebell swing (KBS), emphasizing cyclical, explosive hip extension in the horizontal plane, aligns with movement- and velocity-specificity of sprinting. The present study examined the effect of an eight-week KBS intervention on sprinting in recreationally-active females, in comparison to an eight-week intervention using the stiff-legged deadlift (SDL). Following a pre-testing session measuring 30 meter sprint and countermovement vertical jump performance, participants were divided evenly by sprint time into KBS (n=8) and SDL (n=10) cohorts. Following familiarization with the exercises, KBS met twice weekly to perform swings using the Tabata interval (20s work, …

Inter-Investigator Reliability Of Anthropometric Prediction Of 1rm Bench Press In College Football Players, Jerry L. Mayhew

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 427-436, 2016. The purpose of this study was to determine the effect of inter-investigator differences in anthropometric assessments on the prediction of one-repetition maximum (1RM) bench press in college football players. Division-II players (n = 34, age = 20.4 ± 1.2 y, 182.3 ± 6.6 cm, 99.1 ± 18.4 kg) were measured for selected anthropometric variables and 1RM bench press at the conclusion of a heavy resistance training program. Triceps, subscapular, and abdominal skinfolds were measured in triplicate by three investigators and used to estimate %fat. Arm circumference was measured around a flexed biceps …

Cardiovascular Responses Between Low Cadence/High Force Vs. High Cadence/Low Force Cycling, Randy G. Canivel, Frank B. Wyatt

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 419-426, 2016. The purpose of this study was to determine the cardiovascular responses during sustained power output comparing low cadence (LC) high force (HF) vs. high cadence (HC) Low force (LF) cycle ergometry. Nine participants (N = 9) volunteered for this study. Participants signed a Human Participants consent form. A power output of 150 watts (W) for 20 minutes was estimated as the sustainable workload. Participants were instructed to pedal steadily at 50 rpm (LC) or 100 rpm (HC) with resistance adjusted to sustain 150 W. The following measures were obtained each minute during …

The Effect Of Metronome Use On Heart Rates And Rpe For Drum Corps Percussionists, Danielle Dye, Vaughn Barry

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 412-418, 2016. The purpose was to evaluate if playing a musical selection without a metronome would affect the heart rates and rate of perceived exertion for drum corps percussionists. Heart rate and RPE values were collected from 11 battery and 12 front ensemble members from one drum corps. The music consisted of two portions of the show at different tempos of 96 and 176 beats per minute. The music was performed at "standstill" with the battery members lined up behind the front ensemble marching, but not moving around or carrying their instruments. The participants …

Adolescents’ Perception Of The Psychosocial Factors Affecting Sustained Engagement In Sports And Physical Activity, James Gavin, Madeleine Mcbrearty, Kit Malo, Michael Abravanel, Tatiana Moudrakovski

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 384-411, 2016. The purpose of this study was to explore adolescents’ perceptions of psychosocial influences – personal characteristics, environmental factors and behavioural undertakings – influencing their prolonged involvement in sports and physical activity (PA). A qualitative approach was adopted wherein 16 adolescents (8 boys, 8 girls; mean age 15.9 years), who had been physically active for at least the last 8 years, and sixteen adults identified as their ‘parents’ or ‘guardians’ participated in semi-structured interviews. Interviews were transcribed verbatim and coded using the HyperRESEARCH software. Data were analysed using thematic analysis procedures. Four main …

The Effect Of Skinfold On The Assessment Of The Mean Power Frequency At The Fatigue Threshold, Alyssandra N. Baniqued,, Jorge M. Zuniga, Thomas C. Strunc, Katie M. Keenan, Agrini K. Boken, Jeff J. Anderson

International Journal of Exercise Science

International Journal of Exercise Science 9(4): 376-383, 2016. The purpose of this study was to determine if the amount of subcutaneous tissue over the quadriceps affects the assessment of mean power frequency at the fatigue threshold (MPF_FT). It was hypothesized that greater skinfold values will result in lower power outputs associated to the MPF_FT. Fourteen adults (Mean ± SD age = 20.7 ± 0.99; body weight = 72.8 ± 12.6 kg) performed an incremental cycle ergometry test to exhaustion while surface electromyographic (EMG) signals were measured from the vastus lateralis. The skinfold thickness of each leg …

An Excitatory Cortical Feedback Loop Gates Retinal Wave Transmission In Rodent Thalamus, Yasunobu Murata, Matthew T. Colonnese

Pharmacology and Physiology Faculty Publications

Spontaneous retinal waves are critical for the development of receptive fields in visual thalamus (LGN) and cortex (VC). Despite a detailed understanding of the circuit specializations in retina that generate waves, whether central circuit specializations also exist to control their propagation through visual pathways of the brain is unknown. Here we identify a developmentally transient, corticothalamic amplification of retinal drive to thalamus as a mechanism for retinal wave transmission in the infant rat brain. During the period of retinal waves, corticothalamic connections excite LGN, rather than driving feedforward inhibition as observed in the adult. This creates an excitatory feedback loop …

Neuromuscular Alterations After Ankle Sprains: An Animal Model To Establish Causal Links After Injury, Lindsey K. Lepley, Patrick O. Mckeon, Shane G. Fitzpatrick, Catherine L. Beckemeyer, Timothy L. Uhl, Timothy A. Butterfield

Physical Therapy Faculty Publications

Context: The mechanisms that contribute to the development of chronic ankle instability are not understood. Investigators have developed a hypothetical model in which neuromuscular alterations that stem from damaged ankle ligaments are thought to affect periarticular and proximal muscle activity. However, the retrospective nature of these studies does not allow a causal link to be established.

Objective: To assess temporal alterations in the activity of 2 periarticular muscles of the rat ankle and 2 proximal muscles of the rat hind limb after an ankle sprain.

Design: Controlled laboratory study.

Setting: Laboratory.

Patients or Other Participants: …

Effects Of Short‐Term Free‐Weight And Semi‐Block Periodization Resistance Training On Metabolic Syndrome, Mark A. South, Andrew S. Layne, N. Travis Triplett, William A. Sands, Satoshi Mizuguchi, W. Guy Hornsby, Ashley Kavanaugh, Michael H. Stone

ETSU Faculty Works

The effects of short-term resistance training on performance and health variables associated with prolonged sedentary lifestyle and metabolic syndrome (MS) were investigated. Resistance training may alter a number of health-related, physiological, and performance variables. As a result, resistance training can be used as a valuable tool in ameliorating the effects of a sedentary lifestyle including those associated with MS. Nineteen previously sedentary subjects (10 with MS and 9 with nonmetabolic syndrome [NMS]) underwent 8 weeks of supervised resistance training. Maximum strength was measured using an isometric midthigh pull and resulting force-time curve. Vertical jump height (JH) and power were measured …

Stable Engraftment Of Bifidobacterium Longum Ah1206 In The Human Gut Depends On Individualized Features Of The Resident Microbiome, María X. Maldonado-Gómez, Inés Martínez, Francesca Bottacini, Amy O’Callaghan, Marco Ventura, Douwe Van Sinderen, Benjamin Hillmann, Pajau Vangay, Dan Knights, Robert W. Hutkins, Jens Walter

Department of Food Science and Technology: Faculty Publications

Live bacteria (such as probiotics) have long been used to modulate gut microbiota and human physiology, but their colonization is mostly transient. Conceptual understanding of the ecological principles as they apply to exogenously introduced microbes in gut ecosystems is lacking. We find that, when orally administered to humans, Bifidobacterium longum AH1206 stably persists in the gut of 30% of individuals for at least 6 months without causing gastrointestinal symptoms or impacting the composition of the resident gut microbiota. AH1206 engraftment was associated with low abundance of resident B. longum and underrepresentation of specific carbohydrate utilization genes in the pre-treatment microbiome. …

Abiotic Factors Contributing To The Survival Of Three Tick Species In Southeastern Virginia, Amblyomma Americanum (Lone Star Tick), Dermacentor Variabilis (American Dog Tick), And Amblyomma Maculatum (Gulf Coast Tick), Lindsey A. Bidder

Biological Sciences Theses & Dissertations

Amblyomma americanum, Amblyomma maculatum, and Dermacentor variabilis are hard-bodied ticks in the Hampton Roads area of southeastern Virginia. This study consisted of two field projects focused on these tick species. To estimate the off-host survival of local tick species, a capture-mark-recapture (CMR) study was performed. An environmental survival study was performed to quantify the ability of these three tick species to survive in situ. Four field sites were used in the Hampton Roads region covering a variety of habitat types and vegetation; specifically two drier, upland field sites and two flood-prone sites. CMR was conducted from May through …