Physiology Commons^™

Does Epa Cause A Decrease In Inflammation Of Bend.3 Cells Through Ffar4?, Clay J. Weidenhamer

Masters Theses

Atherosclerosis is an inflammatory disease initiated by low and oscillatory shear stress on the endothelium. The inflammatory process recruits leukocytes to the vessel wall by expression of the adhesion molecule VCAM-1. Activation of the NF-κB inflammatory signaling pathway is responsible for the increase in VCM-1 expression. Omega 3 FAs, such as EPA, reduce the risk of atherosclerosis by decreasing this inflammatory response. The pathway by which omega 3 FAs is proposed to inhibit inflammation includes activating FFAR4 to decrease NF-κB activation thereby reducing expression of adhesion molecules. We hypothesized that treatment of endothelial cells with 30 μM EPA would decrease …

Intravesical Cd74 And Cxcr4, Macrophage Migration Inhibitory Factor (Mif) Receptors, Mediate Bladder Pain, Shaojing Ye, Fei Ma, Dlovan F. D. Mahmood, Katherine L. Meyer-Siegler, Raymond E. Menard, David E. Hunt, Lin Leng, Richard Bucala, Pedro L. Vera

Physiology Faculty Publications

BACKGROUND: Activation of intravesical protease activated receptor 4 (PAR4) leads to release of urothelial macrophage migration inhibitory factor (MIF). MIF then binds to urothelial MIF receptors to release urothelial high mobility group box-1 (HMGB1) and elicit bladder hyperalgesia. Since MIF binds to multiple receptors, we investigated the contribution of individual urothelial MIF receptors to PAR4-induced HMGB1 release in vivo and in vitro and bladder pain in vivo.

METHODOLOGY/PRINCIPAL FINDINGS: We tested the effect of intravesical pre-treatment with individual MIF or MIF receptor (CD74, CXCR4, CXCR2) antagonists on PAR4-induced HMGB1 release in vivo (female C57/BL6 mice) and in vitro (primary …

Immune Cell Profile In The Epididymis Of Leptin And Leptin Receptor Knockout Mice, Hilary Skalski

Masters Theses

Genetically-modified mice lacking the leptin hormone (Ob) or leptin receptor (Db) have reduced testosterone and decreased sperm production leading to infertility. Leptin receptors are also expressed by immune cells, suggesting leptin can regulate immune responses. Recent studies have focused on identifying the immune cells present within the epididymis and found cells expressing markers typically associated with dendritic and macrophage cells. These cells likely function against infection and to induce tolerance to sperm. The effects of leptin on the immune system and the presence of immune cells within the epididymis raises the possibility that alterations in the immune cell profile and/or …

Reduced Injury Prevalence In Soccer Athletes Following Gps Guided Acclimatization, Montgomery Bertschy, Jeffrey Howard, Sakiko Oyama, Kelly Cheever

International Journal of Exercise Science

International Journal of Exercise Science 14(7): 1070-1077, 2021. GPS technology has been used to retrospectively correlate injury risk to changes in training load, however the use of GPS technology to plan and monitor training load over an acclimatization period to prevent musculoskeletal injury remains unexplored. This article reports the utility of GPS technology to help develop and monitor incremental increases in training load while transitioning from off-season to in season to reduce musculoskeletal injury. A series of daily minimum standards were established based on observed training loads in year 1 to gradually acclimate soccer athletes over a 5-week period prior …

Effects Of Different Types Of Lower Body Resistance Exercise On Upper-Body Strength In Men And Women, With Special Reference To Anabolic Hormones, Johan Jakobsson, Apostolos Theos, Christer Malm

International Journal of Exercise Science

International Journal of Exercise Science 14(3): 1052-1069, 2021. Resistance exercise has been shown to induce an acute hormonal response. The purpose of this study was to examine upper-body adaptations and the endocrine response to strength training in men and women when subjected to two different types of lower-body resistance training protocols. Nine males and eight females were assigned to either a Heavy Load (HL) (N = 10) or Mixed Load (ML) (N = 7) training routine three times per week for ten weeks. The HL-group executed low-volume, high-load resistance exercise for both lower and upper-body (4-6 reps at …

Threshold Concentration And Random Collision Determine The Growth Of The Huntingtin Inclusion From A Stable Core, Sen Pei, Theresa C. Swayne, Jeffrey F. Morris, Lesley Emtage

Publications and Research

The processes underlying formation and growth of unfolded protein inclusions are relevant to neurodegenerative diseases but poorly characterized in living cells. In S. cerevisiae, inclusions formed by mutant huntingtin (mHtt) have some characteristics of biomolecular condensates but the physical nature and growth mechanisms of inclusion bodies remain unclear. We have probed the relationship between concentration and inclusion growth in vivo and find that growth of mHtt inclusions in living cells is triggered at a cytoplasmic threshold concentration, while reduction in cytoplasmic mHtt causes inclusions to shrink. The growth rate is consistent with incorporation of new material through collision and coalescence. …

Comparative Analysis Of Longevity And Stress Resiliency Benefits Of Supplementation With High-Antioxidative Capacity Juices In Organisms That Are Representative Of Humans, Meghan Long

Honors Scholar Theses

Neurodegenerative disease presents itself in a complex array of illnesses that is increasingly affecting the geriatric population. There has not been research on a common cause since there are a culmination of reasons that are probable ranging from genetic to environmental factors. Antioxidants have been known to prevent and protect against the damage that is otherwise caused by reactive oxygen species (ROS). Thus, high-capacity antioxidant juices supplemented into the diet of animal models such as Caenorhabditis elegans, Drosophila melanogaster, rats, and humans will allow for the study of the possible benefits.

Although a conclusion and common causation on the usage …

Mechanism Of The Superoxide Dismutase Mimic Mntnbuoe-2-Pyp5+ And Its Nanoformulation On Blood Pressure Regulation, Sarah L. Schlichte

Theses & Dissertations

It is well-established that reactive oxygen species (ROS), particularly superoxide (O₂^·-), produced in the vasculature and brain contribute to the pathogenesis of hypertension. We, and others, have previously reported that in vivo scavenging of O₂^·- via overexpression of superoxide dismutase (SOD) protein or SOD mimics decreases blood pressure in hypertensive animals. While few SOD mimics have transitioned to clinical trials, MnTnBuOE-2-PyP⁵⁺ (BuOE), a manganese porphyrin SOD mimic, is currently in clinical trials for normal tissue protection in cancer patients exposed to radiation therapy. Furthermore, our collaborators developed a nanoformulated BuOE (nanoBuOE) in which BuOE …

The Effect Of Compression Socks On Maximal Exercise Performance And Recovery In Insufficiently Active Adults, Alexander H.K. Montoye, Alexandra A. Mithen, Haven L. Westra, Summer S. Besteman, Brian C. Rider

International Journal of Exercise Science

International Journal of Exercise Science 14(7): 1036-1051, 2021. In athletic populations, compression socks (CS) may improve exercise performance recovery. However, their potential to improve performance and/or recovery following exercise in non-athletic populations is unknown. Our study evaluated the effects of CS on exercise performance and recovery from a graded maximal treadmill test. Insufficiently active adults (n = 10, 60% female, average physical activity ~60 minutes/week) performed two graded maximal exercise tests; one while wearing below-knee CS, and the other trial with regular socks (CON). Order of trials was randomized. For both trials, heart rate, lactate, and rating of perceived …

Epoc Comparison Between Resistance Training And High-Intensity Interval Training In Aerobically Fit Women, Beau Kjerulf Greer, Julie O'Brien, Lyndsey Hornbuckle, Lynn Panton

International Journal of Exercise Science

International Journal of Exercise Science 14(2): 1027-1035, 2021. Previous research has shown that various modes of exercise may elicit significant increases in resting metabolism for up to 24 hours post-exercise, but typically using untrained or moderately active subjects. The purpose of the present study was to compare excess post-exercise oxygen consumption (EPOC) between circuit-style resistance training (RT) and high-intensity interval training (HIIT) in young, aerobically fit women. During the follicular phase of the menstrual cycle, seven participants reported to the laboratory for evening and morning baseline resting metabolic rate (RMR) measurements via indirect calorimetry. Participants fasted and slept overnight in …

Mejore Su Salud, Celina Wille, Laura Licon, Laura Paola Johnson, Anny Galvin

All Current Publications

Create Better Health/Mejore su salud es una versión cultural adaptada de Create Better Health evaluada por expertos y escrita en español, Create Better Health es un currículo del programa Create Better Health Utah SNAP-Ed originalmente adaptado de Creates: Essentials in Creating Family Meals por Debra Christofferson, M.D.A., R.D., C.D. Los miembros del equipo de publicación de la versión en inglés son: Casey Coombs, M.S., R.D., C.D., subdirectora de Create Better Health Utah; Jaqueline Neid-Avila, M.D.A., R.D., C.D .; y editores / colaboradores Heidi LeBlanc, M.S., directora de Create Better Health Utah.

Reverse Myocardial Remodeling In Hypertrophic Cardiomyopathy: Little Explored Benefit Of Exercise, Jefferson Petto, Edna C. De Oliveira, Raissa Va De Almeida, Alice M. De Oliveira, Danielly Sn Do Amaral, Bráulio Jb De Pinna Jr.

International Journal of Exercise Science

International Journal of Exercise Science 14(2): 1018-1026, 2021. Hypertrophic cardiomyopathy (HCM) is an autosomal dominant disease that causes myocardial remodeling. Physical exercise (PE) is a therapeutic resource used in Supervised Cardiac Rehabilitation (SCR) to improve Quality of Life (QL), reducing cardiovascular morbidity and mortality. Therefore, the aim of this study is to report how SCR using a personalized exercise prescription, promoted Reverse Myocardial Remodeling (RMR), improved functionality and QL of a patient with HCM. This is a case report of a 43-year-old sedentary female patient with a Body Mass Index (BMI) of 24.7 kg/m². The patient was diagnosed …

Identifying The Molecular Pathways That Drive Retinal Degeneration In The Childhood Neurodegenerative Disease: Mucolipidosis Type Iv, Michael Pamonag

Masters Theses, 2020-current

Humans, like many other vertebrates, possess five Aristotelian senses (vision, olfaction, hearing, taste, and touch) which we use to experience and navigate our environment. Our visual system is the only source of light detection and light signaling in our bodies. This makes our visual system essential for detecting movement, distance, time of day, and seasonal changes in the length of days within our environment. The visual systems of most animals are designed to capture photons of visible light and convert that energy into a neurological signal (visual signal) to be transmitted to brain regions responsible for visual perception1 . This …

The Influence Of Incubation Conditions On Oxygen Consumption During The Development Of Pantherophis Guttatus, Celeste Gallardo

Electronic Theses and Dissertations

The flexible shell of some oviparous reptiles has led to differences in nutrient mobilization and water relations when compared to their rigid-shelled counterparts. Flexible-shelled eggs gain more water during development, and because of the shell structure, yolk calcium content is higher than that of eggshells. When water availability is altered for reptiles with flexible-shelled eggs, differences in both energy and nutrient utilization are observed. This study was designed to determine the baseline metabolic rate during development for the snake, Pantherophis guttatus under normal conditions, and to observe how changes in eggshell calcium and water availability impact embryonic oxygen consumption. Eggshell …

Anatomy And Physiology Preparatory Course Textbook (2nd Edition), Carlos Liachovitzky

Open Educational Resources

The goal of this preparatory textbook is to give students a chance to become familiar with some terms and some basic concepts they will find later on in the Anatomy and Physiology course, especially during the first few weeks of the course.

Organization and functioning of the human organism are generally presented starting from the simplest building blocks, and then moving into levels of increasing complexity. This textbook follows the same presentation. It begins introducing the concept of homeostasis, then covers the chemical level, and later on a basic introduction to cellular level, organ level, and organ system level. This …

Modulation Of Glucose Homeostasis By Nucleotide P2y2 Receptor And Biological Sex, Hailee Anne Marino

Graduate Theses/Dissertations

Recent insights into the pathological role of Nucleotide P2Y₂ receptor suggest P2Y₂R involvement in high fat diet-induced obesity and potentiates insulin resistance. However, these recent insights do not demonstrate how P2Y₂R modulates glucose homeostasis under physiological conditions. Further, it remains unknown how sex biological factors influence P2Y₂R receptor signaling in the regulation of glucose homeostasis. The research objective for the present study is to elucidate the novel roles of P2Y₂ in fasting blood glucose and glucose tolerance (basal insulin sensitivity) under resting conditions in males and females. We expected that under physiological …

Exercise Training Rescues Increased Brain Isf Aβ Caused By Chronic Isolation Stress In App/Ps1 Transgenic Mice, Lydia M. Holtmann

Graduate Theses/Dissertations

Alzheimer’s Disease (AD) is a progressive brain disorder that destroys memory and cognition thought to be initiated through the build-up of the amyloid-beta (A) peptide. The A peptide aggregates, slowly forming into insoluble plaque substances that destroy the brain and worsen patient’s symptoms over time. Studies have shown that chronic isolation stress (CIS) increases the A peptide soluble and insoluble levels in the brain and that exercise training decreases these levels in transgenic mouse models of AD. We sought to determine if an exercise training regimen would rescue the increase in Aβ levels caused by CIS in the APP/PS1 transgenic …

Ecological Correlates Of Alligator Snapping Turtle Bite Performance, Ashley Herrin Gagnon

Graduate Theses/Dissertations

The Alligator Snapping Turtle (Macrochelys temminckii) is one of many turtle species facing conservation challenges. Nearly extirpated in the 1980s, it is currently the subject of a head-start initiative, of which, any lasting behavioral or physiological effects were—until now—unknown! To evaluate the ability of captive-reared individuals to excel in natural habitats, and to foresee any future research or conservation challenges regarding this animal, I explored a suite of variables that influence bite performance and behaviors including captive or free-ranging status, and environmental conditions including body temperature and season. My results indicated that free-ranging M. temminckii outperform those residing …

Understanding The Pathogenesis Of Renal Medullary Carcinoma, Melinda Soeung

Dissertations and Theses (Open Access)

Renal medullary carcinoma (RMC) is a lethal cancer that predominantly affects young individuals with sickle cell trait (SCT). It is not currently understood why RMC only affects certain individuals with SCT. We found that patients with RMC more frequently participated in high-intensity exercise than matched controls. Using mouse models of SCT, we demonstrated the significant increase of renal hypoxia in the right kidney following high- but not moderate-intensity exercise. We also demonstrated in cell culture studies that SMARCB1 is ubiquitinated for proteasome-mediated degradation in hypoxia, and the re-expression of SMARCB1 leads to compromised proliferation in renal cells specifically in the …

Histological Analysis And Gene Expression Of Satellite Cell Markers In The Pectoralis Major Muscle In Broiler Lines Divergently Selected For Percent 4-Day Breast Yield, Sara K. Orlowski, Sami Dridi, Elizabeth S. Greene, Cynthia S. Coy, Sandra G. Velleman, Nicholas B. Anthony

Poultry Science Faculty Publications and Presentations

Muscle development during embryonic and early post-hatch growth is primarily through hyperplastic growth and accumulation of nuclei through satellite cell contribution. Post-hatch, muscle development transitions from hyperplasia to hypertrophic growth of muscle fibers. Commercial selection for breast yield traditionally occurs at ages targeting hypertrophic rather than hyperplastic growth. This has resulted in the production of giant fibers and concomitant challenges with regard to muscle myopathies. The current study investigates the impact of selection during the period of hyperplastic growth. It is hypothesized that selection for percentage breast yield during hyperplasia will result in an increased number of muscle cells at …

Physical Performance Assessments Of Strength And Power In Women Collegiate Athletes, Brian Guthrie, Jennifer B. Fields, Brian Thompson, Margaret T. Jones

International Journal of Exercise Science

International Journal of Exercise Science 14(6): 984-993, 2021. Limited research exists on physical performance assessments for women collegiate athletes. The current cross-sectional study compared field-based tests of muscular strength and power and investigated their relationship. Sports included field hockey, volleyball, soccer, and softball. Tests of one repetition maximum (1-RM) back squat, 1-RM bench press, vertical jump, and standing long jump were administered. A one-way analysis of variance (ANOVA) assessed differences across sports. Bivariate Pearson correlation coefficients examined relationships among tests. It was hypothesized sports with a higher anaerobic nature (volleyball, softball) would outperform those with higher aerobic nature (field hockey, …

Nontraumatic Injuries In The Ncaa: Collegiate Football Strength Coaches Should Exercise Caution This Off-Season, Andreas Stamatis, Marshall Magnusen

International Journal of Exercise Science

International Journal of Exercise Science 14(6): 980-983, 2021. Strength and conditioning coaches were allegedly involved in pre-COVID-19 nontraumatic injuries/deaths (e.g., exertional heat illness, exertional rhabdomyolysis, cardiorespiratory failure) of NCAA (mainly football) student-athletes during off-season training sessions. During the COVID-19 health crisis, non-pharmaceutical interventions against the SARS-CoV-2 virus included suspension of NCAA seasons, which led to student-athletes exhibiting lower levels of mental health. All NCAA championships have now been reinstated. This summer the off-season is unique, because as several programs had stopped practicing/competing for several months, it may foster additional threats to the wellbeing of the student-athletes. Immediate supplementary action may …

Combined Physical Training Increases Plasma Brain-Derived Neurotropic Factor Levels, But Not Irisin In People Living With Hiv/Aids, Joice Cristina Dos Santos Trombeta, Roberto Carlos Vieira Junior, Cláudia Regina Cavaglieri, Ivan Luiz Padilha Bonfante, Jonato Prestes, Ramirez Alsamir Tibana, Morteza Ghayomzadeh, Vinicius Carolino Souza, Seyedahmad Seyedalinaghi, James Wilfred Navalta, Fabrício Azevedo Voltarelli

International Journal of Exercise Science

International Journal of Exercise Science 14(3): 1004-1017, 2021. This study evaluated plasma levels of brain-derived neurotropic factor (BDNF), irisin, and lactate in people living with HIV/AIDS who completed a combined physical training program. Nineteen HIV⁺ participants (age: 39.60 ± 10.96 years; carrier time: 7.75 ± 7.88 years; time of ART: 6.41 ± 5.93 years) performed strength/aerobic training (combined physical training) in the same session for 8 weeks and levels of BDNF, irisin, and lactate were assessed. BDNF (pg/mL) was higher post-CPT (Pre: 1258.73 ± 372.30; Post: 1504.17 ± 322.30; p < 0.001). Irisin (ng/mL) showed no change (Pre: 115.61 ± 72.41; Post: 125.87 ± 81.14; p = 0.973). There was positive correlation between irisin …

Beta-Alanine Supplementation For Four Weeks Increases Volume Index And Reduces Perceived Effort Of Resistance-Trained Men: A Pilot Study., Julio Benvenutti Bueno De Camargo, Felipe Brigatto, Rafael Zaroni, Moises Germano, Raphael Conceicao, Alexandre Martins, Marcelo Aoki, Tiago Braz, Charles Lopes

International Journal of Exercise Science

International Journal of Exercise Science 14(2): 994-1003, 2021. The aim of the present study was to assess the short-term effects of four weeks of beta-alanine supplementation (BA) (6.4 g/day) on the total volume performed and perceived effort of resistance-trained individuals. Sixteen trained men (age: 27.3 ± 5.0 years, height: 1.78 ± 0.1 cm, total body mass: 84.3 ± 8.4 kg, RT experience: 5.9 ± 3.3 years) were allocated in one of the following groups: BA or Placebo (PLA). In addition, during the same period, participants were submitted to a resistance training program. Volume index (VI) and the rate of perceived …

A Systematic Review And Meta-Analysis On The Efficacy Of Stem Cell Therapy On Bone Brittleness In Mouse Models Of Osteogenesis Imperfecta, Lauren Battle, Shoshana Yakar, Alessandra Carriero

Publications and Research

There is no cure for osteogenesis imperfecta (OI), and current treatments can only partially correct the bone phenotype. Stem cell therapy holds potential to improve bone quality and quantity in OI. Here, we conduct a systematic review and meta-analysis of published studies to investigate the efficacy of stem cell therapy to rescue bone brittleness in mouse models of OI. Identified studies included bone marrow, mesenchymal stem cells, and human fetal stem cells. Effect size of fracture incidence, maximum load, stiffness, cortical thickness, bone volume fraction, and raw engraftment rates were pooled in a random-effects meta-analysis. Cell type, cell number, injection …

Evaluation Of Load Velocity Profiles With Varying Warm-Up Sets And Relative Intensities, Gabriel L. Perez, Samantha V. Narvaez, Joshua Carrillo, Misael Duque, Noel F. Medrano, Brett S. Nickerson

International Journal of Exercise Science

International Journal of Exercise Science 14(4): 971-979, 2021. The purpose of this study was to determine whether the number of warm-up sets and relative intensity impacts the prediction of velocity-based one-repetition maximum (1RM) values. Twenty resistance-trained individuals (males: n = 10, females: n = 10) participated in this study. Warm-up sets consisted of subject’s bench-pressing loads at 50 (five-repetitions), 70 (three-repetitions), and 90% (one-repetition) of estimated 1RM. A maximum of four attempts were performed to determine 1RM, while recording mean concentric velocity (MCV)using a linear position transducer during warm-up and 1RM trials in order to develop load-velocity profiles. Specifically, four …

Transcranial Direct Current Stimulation With The Halo Sport Does Not Improve Performance On A Three-Minute High Intensity Cycling Test, Collin Garner, Rachel Dykstra, Nicholas J. Hanson, Michael G. Miller

International Journal of Exercise Science

International Journal of Exercise Science 14(3): 962-970, 2021. Transcranial direct current stimulation (tDCS) uses a weak electrical current that is sent through the cerebral cortex. The Halo Sport headphones are a user-friendly form of tDCS that is implemented by many athletes purportedly to improve performance. The purpose of this study was to determine the effect of tDCS, using the Halo Sport, on performance variables associated with a high-intensity three-minute cycling test. Eighteen healthy, active individuals (ten men, eight women) volunteered for this study. The Halo Sport headphones were worn during a 20-minute warmup before completing a high-intensity three-minute cycling test. …

Notch Signaling Represses Cone Photoreceptor Formation Through The Regulation Of Retinal Progenitor Cell States, Xueqing Chen, Mark M. Emerson

Publications and Research

Notch signaling is required to repress the formation of vertebrate cone photoreceptors and to maintain the proliferative potential of multipotent retinal progenitor cells. However, the mechanism by which Notch signaling controls these processes is unknown. Recently, restricted retinal progenitor cells with limited proliferation capacity and that preferentially generate cone photoreceptors have been identified. Thus, there are several potential steps during cone genesis that Notch signaling could act. Here we use cell type specific cis-regulatory elements to localize the primary role of Notch signaling in cone genesis to the formation of restricted retinal progenitor cells from multipotent retinal progenitor cells. Localized …

Late Embryogenesis Abundant Proteins And Mitochondrial Membranes From An Animal Extremophile: Insights Into Severe Water Stress, John Marcus Anderson

LSU Doctoral Dissertations

This dissertation focuses on two major topics germane to mechanisms by which animals tolerate extreme water stress. First, the impact of transgenic expression of late embryogenesis abundant proteins (LEA) on water stress tolerance of the fruit fly Drosophila melanogaster was investigated. The overall aim was to extend current understanding of the protective properties of LEA proteins documented with isolated cells to a desiccation-sensitive organism during exposure to drying and hyperosmotic stress. Four lines of D. melanogaster were created that expressed transgenes encoding selected LEA proteins originally identified in embryos of the extremophile Artemia franciscana. After 80% tissue water loss, …

Temperature-Induced Activation Of The Reproductive Axis Through Melatonin-Mediated Changes In Thyrotropin, Treven J. Winters

Dissertations and Theses

An animal's ability to synchronize life-history events or stages with optimal environmental conditions is paramount to successfully reproducing and maximizing fitness. Additional events including migration, foraging, rearing of young, and emergence from hibernation are all examples of processes under environmental control in one species or another. An exciting new mechanism involving neural thyroid hormone metabolism has been elucidated that links environmental control to the neuroendocrine reproductive axis. In birds and mammals with seasonal breeding cycles, this neuroendocrine pathway is activated by photoperiod-induced changes in thyroid stimulating hormone (TSH, also known as thyrotropin) production within the pars tuberalis region of the …