Physiology Commons^™

Higher Body Mass Index Values Do Not Impact Physical Function And Lower-Extremity Muscle Strength Performance In Active Older Individuals, Vitor A.A.A. Siqueira, Emerson Sebastião, Clayton L. Camic, Dalmo R.L. Machado

International Journal of Exercise Science

International Journal of Exercise Science 15(3): 330-340, 2022. This study examined the potential impact of BMI on physical function and lower-extremity muscle strength (leg extension and flexion peak torque) performance in active/trained older individuals. Sixty-four active/trained older individuals were enrolled, and later allocated to groups according to BMI categories (normal [≤ 24.9 kg/m²], overweight [25 to 29.9 kg/m²] and obese [≥ 30 kg/m²]). Sixty-four active/trained older individuals were enrolled, and later allocated to groups according to BMI categories (normal [≤ 24.9 kg/m²], overweight [25 to 29.9 kg/m²] and obese [≥ …

Us Army Reserve Officers’ Training Corps Cadets’ Knowledge Of Exercise-Related Musculoskeletal Injuries, Joshua D. Wooldridge, Noelle M. Selkow, Todd A. Mcloda, Kara N. Radzak

International Journal of Exercise Science

International Journal of Exercise Science 15(3): 300-312, 2022. Musculoskeletal injuries, especially resulting from physical training, are a significant threat to military readiness. Due to costs related to treating injuries and the high probability of chronic, recurrent injuries, prevention should be a primary focus to maximize human performance and military success. However, in the US Army, many personnel are uninformed on injury prevention topics, and no research has identified injury prevention knowledge gaps in military leaders. This study examined the current knowledge of US Army ROTC cadets on injury prevention topics. This cross-sectional study was conducted at two university ROTC programs …

Glycogen Kinetics Of Wistar Rats: Different Exercise Intensities And Tissue Analyzed Influence, Carlos Dellavechia De Carvalho, Carlos Augusto Kalva-Filho, Alisson Luiz Da Rocha, Adelino Sanchez Ramos Da Silva, Marcelo Papoti

International Journal of Exercise Science

International Journal of Exercise Science 15(2): 289-299, 2022. The aim of this study is to verify the influence of the intensity on muscle and hepatic glycogen depletion and recovery kinetics of Wistar rats, submitted to three acute training sessions with equalized loads. 81 male Wistar rats performed an incremental test to determine maximal running speed (MRS) and divided into 4 groups: baseline group (Control; n = 9); low intensity training session (G_Z1; n = 24; 48 minutes at 50% of MRS); moderate intensity group (G_Z2; n = 24; 32 minutes at 75% of MRS) and high …

An Analysis Of The Body Drag Test In Law Enforcement Recruits With Consideration To Current Population Demographics, Robert G. Lockie, Matthew R. Moreno, J. Jay Dawes, Robin Marc Orr, Karly A. Rodas, Joseph M. Dulla

International Journal of Exercise Science

International Journal of Exercise Science 15(7): 276-288, 2022. An essential job task for law enforcement officers is a body drag, where they must drag a person from a hazardous environment. In California, a 9.75-m body drag with a 74.84-kg dummy must be completed within 28 s to graduate academy. This mass is less than the average US adult, which may suggest it should be increased. This has not happened due to concerns regarding a potential increase in recruit injuries and failure rates. However, if recruits can complete the drag without formal training, this could leave scope for increasing the mass. …

A Comparison Of Three Different Warm-Ups On 800-Meter Running Performance In Elite Division I Track Athletes – A Pilot Study, Roger Keesling, Andreas N. Kavazis, Benjamin Wax, Matthew W. Miller, Brad Vickers

International Journal of Exercise Science

International Journal of Exercise Science 14(6): 1400-1407, 2021. Track and Field athletes perform different types of warm-ups at varying levels of volume and/or intensity prior to competition. Theoretically, this prepares sport specific muscles for activity by increasing muscle temperature, thus mitigating the chance for injury. There is a paucity of information regarding the optimum level for warm-ups regarding maximizing performance in middle distance events. The aim of this study was to examine the effects of three different warm-ups on 800-meter performance. Thirteen Division I student-athletes (seven males and six females) from the Southeastern Conference (SEC) who were middle distance runners …

Apoe Genetics Influence Murine Gut Microbiome, Diana J. Zajac, Stefan J. Green, Lance A. Johnson, Steven Estus

Physiology Faculty Publications

Apolipoprotein E (APOE) alleles impact pathogenesis and risk for multiple human diseases, making them primary targets for disease treatment and prevention. Previously, we and others reported an association between APOE alleles and the gut microbiome. Here, we evaluated effects of APOE heterozygosity and tested whether these overall results extended to mice maintained under ideal conditions for microbiome analyses. To model human APOE alleles, this study used APOE targeted replacement (TR) mice on a C57Bl/6 background. To minimize genetic drift, homozygous APOE3 mice were crossed to homozygous APOE2 or homozygous APOE4 mice prior to the study, and the resulting …

Sex Differences In Endurance Exercise Capacity And Skeletal Muscle Lipid Metabolism In Mice, Lola Holcomb, Patrick Rowe, Caitlin O'Neill, Elizabeth Dewitt, Stephen C. Kolwicz Jr.

Health Sciences Faculty Publications

Previous studies suggest that sex differences in lipid metabolism exist with females demonstrating a higher utilization of lipids during exercise, which is mediated partly by increased utilization of muscle triglycerides. However, whether these changes in lipid metabolism contribute directly to endurance exercise performance is unclear. Therefore, the objective of this study was to investigate the contribution of exercise substrate metabolism to sex differences in endurance exercise capacity (EEC) in mice. Male and female C57BL/6-NCrl mice were subjected to an EEC test until exhaustion on a motorized treadmill. The treadmill was set at a 10% incline, and the speed gradually increased …

Reducing Fear Overgeneralization With Safety Learning: Attention Bias As A Moderator, Boyang Fan

Dissertations, Theses, and Capstone Projects

Anxiety disorders are the most prevalent mental health condition and affect one out of nine individuals around the world. Fear generalization is a neurocognitive mechanism thought to maintain and exacerbate anxiety, and thus is an important target of therapeutic interventions. Yet, intervention research and practice place relatively little emphasis on its importance. Given that a significant proportion of individuals do not respond to extant treatments of anxiety disorders, a strengthened focus on fear generalization may inform the development and personalization of new treatment approaches. Recent notions have linked fear generalization to failures in distinguishing between signals that predict the occurrence …

Muscle Activity And User-Perceived Exertion During Immersive Virtual Reality Exergaming Incorporating An Adaptive Cable Resistance System, David Gomez, Jonathan D. Browne, Abdulrahman Almalouhi, Max Abundex, Jonathan Hu, Sally Nason, Nicholas Kull, Cade Mills, Quincy Harris, Richard Ku, Eric V. Neufeld, Brett A. Dolezal

International Journal of Exercise Science

International Journal of Exercise Science 15(7): 261-275, 2022. The purpose of this exploratory study was to characterize muscle activation via surface electromyography (sEMG), user-perceived exertion, and enjoyment during a 30-minute session of immersive virtual reality (IVR) cable resistance exergaming. Ten healthy, college-aged males completed a signature 30-minute exergaming session using an IVR adaptive cable resistance system that incorporated six traditional compound exercises. Muscle activation (sEMG) was captured during the session with a wearable sEMG system. Rated of Perceived Exertion (RPE) and Physical Activity Enjoyment Scale (PACES) were recorded following the session. Pectoralis major showed the highest activation during chest press, …

The Coxsackievirus And Adenovirus Receptor Has A Short Half-Life In Epithelial Cells, Poornima Kotha Lakshmi Narayan, James M. Readler, Mahmoud S. Alghamri, Trisha L. Brockman, Ray Yan, Priyanka Sharma, Vladislav Snitsarev, Katherine J.D.A Excoffon, Abimbola O. Kolawole

Department of Biology Faculty Scholarship and Creative Works

The coxsackievirus and adenovirus receptor (CAR) is an essential cellular protein that is involved in cell adhesion, cell signaling, and viral infection. The 8-exon encoded isoform (CAREx8) resides at the apical surface of polarized epithelia, where it is accessible as a receptor for adenovirus entering the airway lumen. Given its pivotal role in viral infection, it is a target for antiviral strategies. To understand the regulation of CAREx8 and determine the feasibility of receptor down regulation, the half-life of total and apical localized CAREx8 was determined and correlated with adenovirus transduction. Total and apical CAREx8 has a relatively short half-life …

Gut Microbiome And Metabolome Variations In Self-Identified Muscle Builders Who Report Using Protein Supplements, Lauri O. Byerley, Karyn M. Gallivan, Courtney J. Christopher, Christopher M. Taylor, Meng Luo, Scot E. Dowd, Gregory M. Davis, Hector F. Castro, Shawn R. Campagna, Kristin S. Ondrak

School of Graduate Studies Faculty Publications

Muscle builders frequently consume protein supplements, but little is known about their effect on the gut microbiota. This study compared the gut microbiome and metabolome of selfidentified muscle builders who did or did not report consuming a protein supplement. Twenty-two participants (14 males and 8 females) consumed a protein supplement (PS), and seventeen participants (12 males and 5 females) did not (No PS). Participants provided a fecal sample and completed a 24-h food recall (ASA24). The PS group consumed significantly more protein (118 ± 12 g No PS vs. 169 ± 18 g PS, p = 0.02). Fecal metabolome and …

Relationships Between Physiological And Self-Reported Assessment Of Cancer-Related Fatigue, Trista Olson, Kevin D. Dames, Jeremy D. Smith, Reid Hayward

International Journal of Exercise Science

International Journal of Exercise Science 15(3): 177-190, 2022. The purpose of this study was to evaluate the relationships between subjective, self-reported cancer related fatigue (CRF) and objective measures of muscular strength and fatigability in cancer survivors. A total of 155 cancer survivors (60 ± 13 years of age) completed a questionnaire for the assessment of CRF, along with assessments of handgrip strength, quadriceps strength and fatigability (reduced force/torque). Fatigability was measured by completing 15 maximal isokinetic contractions of the knee extensors (QFI). Spearman’s rho correlation coefficients were calculated as pairwise combinations of the numerical and categorical dependent measures. Categorical variables …

Evaluating The Effect Of Shoes With Varying Mass On Vertical Ground Reaction Force Parameters In Short-Term Running, Bahador Keshvari, Steven Alevras, Juergen Mitternacht, Veit Senner

International Journal of Exercise Science

International Journal of Exercise Science 15(1): 191-205, 2022. Past investigations have revealed that running shoes affect ground reaction force parameters. However, these studies are unclear as to whether these changes, which occur while running in different shoe types of differing masses, are the result of the structural design or the mass of the shoe. The main aim of this study is to evaluate the effect of shoe mass on vertical ground reaction force parameters: active peak and impulse. Methods. 21 male runners (24.52 years old (± 3.09) and 77.13kg (± 7.9)) participated in the experiment. A baseline shoe (BS) …

Affective Responses To Repeated Endurance Training Sessions With Different Intensities: A Randomized Trial, Solfrid Bratland-Sanda, Anine Elieson, Martine Kråkemo, Michael Reinboth

International Journal of Exercise Science

International Journal of Exercise Science 15(5): 152-165, 2022. The purpose was to examine differences in affective responses to repeated sessions of endurance training with different intensities in healthy adults. Thirty young, healthy, and recreationally physically active adults (50% women, age 24.4 ± 6.0 years, VO_2max 48.6 ± 7.4 ml^-1×kg^-1×min^-1, BMI 23.5 ± 2.4 kg×m²) performed a VO_2maxtest. They were randomized to four sessions of either high intensity sprint interval training (SPRINT, n=10, 5 × 30-sec at >95 of HR_peak, 4-min recovery between intervals), high intensity aerobic interval …

Dyadic Effects Of Pokémon Go On Physical Activity And Sedentary Behavior In Mothers And Children, Shirlene D. Wang, Eldin Dzubur, Christine H. Naya, Tyler B. Mason, Genevieve F. Dunton

International Journal of Exercise Science

International Journal of Exercise Science 15(5): 142-151, 2021. Family-based mobile health applications may be an opportunity to increase children’s physical activity (PA) levels. Researchers have highlighted Pokémon GO as a potential model for future PA interventions as it integrates PA with social gamification. This study provides descriptive data on Pokémon GO usage among mothers and their children and examines differences in moderate to vigorous PA (MVPA) over time among individuals playing Pokémon GO compared to non-players using a dyadic subsample from a three-year longitudinal study. After the release of Pokémon Go in July 2016, 156 mother-child dyads completed questionnaires about …

Behavior Change Trajectories And Metabolic Syndrome Risk Factor Clustering During The Transition To College: A Feasibility Pilot Study, Alexandra N. Smith, Lauren Q. Higgins, Simon Higgins

International Journal of Exercise Science

International Journal of Exercise Science 15(5): 125-141, 2022. Metabolic syndrome (MetS) is typically diagnosed in adults; however, MetS risk factors are growing in prevalence during youth and young adulthood. Though the transition from high school to college is associated with adverse changes in lifestyle behaviors that may contribute to MetS risk factor development, the relationship between pre-college MetS risk status and transition-related behavior change is unknown. This prospective study aimed to describe the relationship between pre-college MetS risk status and transition-related behavior change trajectories in college-bound students. Moreover, it aimed to assess the feasibility of the study design, including acceptability …

Acute Effects Of Inter-Set Stretching On Performance And Metabolic Parameters Of Resistance-Trained Men, Julio Benvenutti Bueno De Camargo, Felipe Alves Brigatto, Moises Diego Germano, Raphael Machado Conceicao, Ivan Teixeira, Rebecca Duarte, Luiza Fellet, Tiago Volpi Braz, Jonato Prestes, Paulo Henrique Marchetti, Jeffrey Willardson, Charles Ricardo Lopes

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 231-244, 2022. The present study investigated acute muscular performance and metabolic responses to resistance training (RT) with inter-set stretching (ISS). Seventeen resistance-trained men (age: 30.0 ± 5.6 years; body mass: 81.8 ± 13.4 kg; height: 173 ± 6.2 cm; RT experience: 4.6 ± 1.7 years) completed the ISS and traditional training (TT) protocol seven days apart in a randomized order. In both protocols, 7 sets of the seated cable fly exercise were performed with a 10-repetition maximum (10-RM) load. During the ISS protocol, subjects completed inter-set passive static stretching of the agonist muscles for …

A Submaximal Field Test Of Aerobic Capacity Does Not Accurately Reflect Vo2max In Career Firefighters, Davis Hale‡, Roger O. Kollock, Jacob Thomas, Gabriel J. Sanders, Will Peveler, Andrew Mangan, Blake Landon

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 221-230, 2022. Adequate aerobic capacity is crucial to maintaining firefighter safety. The purpose of this study was to compare predicted VO_2max scores from a submaximal and maximal step test. Eighteen career male firefighters from a medium sized urban municipality completed both a submaximal Forestry step test and a maximal laboratory WFI step test. A lack of association (p = .017) and low level of agreement (p = .015) was determined between step test protocols producing a mean bias of ± 5.61 mL.kg-1/min-1 with most scores being overestimated. Use of the Forestry …

Muscle Daily Undulating Periodization For Strength And Body Composition: The Proposal Of A New Model, Douglas Leão Peixoto, Bruno Magalhães De Castro, Anderson Geremias Macedo, Christiano Bertoldo Urtado, Paulo Soares Lima, Richard Leite, James W. Navalta, Jonato Prestes

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 206-220, 2022. The traditional linear periodization model is designed for modifications to be performed over several weeks, whereas alterations in the undulating model are applied on a more frequent basis. The study investigated a novel periodization scheme, the muscle daily undulating periodization model (mDUP). Thirty-seven men were randomly assigned into 2 groups: (a) a group that performed 12 weeks of daily undulating periodization with fix overload (DUP-F) resistance training (n = 19) and (b) a group that performed 12-weeks of muscle daily undulating periodization with variation overload (mDUP) (n = 18). Body …

Analysis Of Total And Segmental Body Composition Relative To Fitness Performance Measures In Law Enforcement Recruits, Kyle S. Collins, Bryan Christensen, Robin Marc Orr, Joe Dulla, J. Jay Dawes, Robert G. Lockie

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 245-260, 2022. Law enforcement agencies often test the fitness performance and body composition of incoming recruits. This study investigated the relationships between whole and segmental body composition, and fitness tests in law enforcement recruits. A retrospective analysis of 72 male and 11 female recruits was performed. Bioelectrical impedance analysis (BIA) variables were: lean mass (LM), upper-extremity lean mass (UELM), trunk LM, lower-extremity lean mass (LELM), fat mass (FM), upper-extremity fat mass (UEFM), trunk FM, and lower-extremity fat mass (LEFM). Fitness tests included: vertical jump (VJ), peak anaerobic power (PAPw), 75-yard pursuit run (75PR), push-ups, …

Fluid Restriction Dehydration Increase Core Temperature During Endurance Exercise Compared To Exercise Induced Dehydration, Jessica E. Sansone, Mary S. Guyer, Elizabeth M. Mullin, Brian Thompson

International Journal of Exercise Science

International Journal of Exercise Science 15(2): 166-176, 2022. This study aimed to evaluate the difference in heart rate and core temperature during aerobic exercise between two forms of dehydration: exercise-induced (EI) and fluid restricted (FR). Twenty-two subjects (N = 22; 83.35 ± 13.92 kg) completed the current study, performing a familiarization session, a pre-experimental exercise session, and two exercise testing sessions. The EI exercise trial (81.52 ± 13.72 kg) was conducted after performing exercise in a hot environment to lose three to four percent of body weight and partial rehydration. The FR exercise trial (81.53 ± 14.14 kg) …

The Effects Of A 2-Hour Surfing Session On The Hydration Status Of Male Recreational Surfers, Bryon O'Neill, Eric Leon, James Furness, Ben Schram, Kevin Kemp-Smith

International Journal of Exercise Science

International Journal of Exercise Science 14(6): 1388-1399, 2021. Surfing is a popular sport globally which is performed in varied environmental conditions. With limited research in the field exploring hydration, monitoring the effect of surfing on subject hydration is warranted. The purpose of this study was to determine the relationship between surfing intensity and hydration status. A total of ten recreational male surfers were recruited for this study where hydration status was assessed pre-and post-surf session by measures of body mass (BM) and urine specific gravity (USG). Intensity of the surf session was quantified by Global Positioning Systems and Heart Rate …

Inflammatory Bowel Disease Causing Retroperitoneal Varicosity Mimicking A Renal Artery Aneurysm: A Novel Case Report And Literature Review, Areez Shafqat, Shameel Shafqat, Belal Nedal Sabbah, Abdullah Shaik, Wael Khalil Alfehaid, Syed Shafqat Ul Islam

Medical College Documents

A 17-year-old female presented to our hospital complaining of bloody diarrhea 4-6 times per day for the past month. She was a known case of inflammatory bowel disease noncompliant to her medications. Abdominal computed tomography revealed an unusually dilated mass in the retroperitoneum at L2 vertebral level connecting the lumbar and left renal veins. The renal artery was visualized separately, and a diagnosis of communicating vein varicosity was made. This lesion can be misleading on imaging, hence our aim to disseminate our findings to practicing radiologists. The differential diagnosis of these lesions include retroperitoneal lymphadenopathy, renal artery aneurysms, and testicular …

Navigating The “Covid Hangover” In Physiology Courses, Jennifer E. Schaefer

Biology Faculty Publications

Undergraduate educators and students must navigate lingering aftereffects of the COVID pandemic on education in the 2021–2022 academic year even as COVID continues to impact delivery of undergraduate science education. This article describes ongoing difficulties for undergraduate science, technology, engineering, and mathematics (STEM) students and educators and suggests strategies and easy-to-use resources that may help educators navigate the “COVID hangover” and ongoing COVID-related disruptions.

Comparative Genomics, Evolutionary Epidemiology, And Rbd-Hace2 Receptor Binding Pattern In B.1.1.7 (Alpha) And B.1.617.2 (Delta) Related To Their Pandemic Response In Uk And India, Chiranjib Chakraborty, Ashish Ranjan Sharma, Manojit Bhattacharya, Bidyut Mallik, Shyam Sundar Nandi, Sang-Soo Lee

Journal Articles: Cellular & Integrative Physiology

BACKGROUND: The massive increase in COVID-19 infection had generated a second wave in India during May-June 2021 with a critical pandemic situation. The Delta variant (B.1.617.2) was a significant factor during the second wave. Conversely, the UK had passed through the crucial phase of the pandemic from November to December 2020 due to B.1.1.7. The study tried to comprehend the pandemic response in the UK and India to the spread of the B.1.1.7 (Alpha, UK) variant and B.1.617.2 (Delta, India) variant.

METHODS: This study was performed in three directions to understand the pandemic response of the two emerging variants. First, …

T-Lymphocyte Tyrosine Hydroxylase Regulates T H 17 T-Lymphocytes During Repeated Social Defeat Stress, Safwan K. Elkhatib, Cassandra M. Moshfegh, Gabrielle F. Watson, Adam J. Case

Journal Articles: Cellular & Integrative Physiology

Posttraumatic stress disorder (PTSD) is a debilitating psychiatric disorder which results in deleterious changes to psychological and physical health. Patients with PTSD are especially susceptible to life-threatening co-morbid inflammation-driven pathologies, such as autoimmunity, while also demonstrating increased T-helper 17 (T_H17) lymphocyte-driven inflammation. While the exact mechanism of this increased inflammation is unknown, overactivity of the sympathetic nervous system is a hallmark of PTSD. Neurotransmitters of the sympathetic nervous system (i.e., catecholamines) can alter T-lymphocyte function, which we have previously demonstrated to be partially mitochondrial redox-mediated. Furthermore, we have previously elucidated that T-lymphocytes generate their own catecholamines, and strong …

Validation Of Prmr Equations In A Non-Caucasian Sample – Ethnicity As A Variable In Predicting Resting Metabolic Rate, Erin N. Bannister

Graduate Research Theses & Dissertations

This study intends to identify a predictive resting metabolic rate (pRMR) equation that is predictive of the measured resting metabolic rate (mRMR) in a sample that includes a group of Black Americans. The handful of commonly used pRMR equations, such as the Mifflin- St. Jeor or Harris-Benedict equations, were created without defining the demographics of their populations, while validation of these equations is typically done with almost exclusively Caucasian subjects. Black and brown Americans require the same evidence of precision in predictions of daily energy needs as Caucasian Americans. When applied to non-Caucasian samples, these equations appear in the literature …

Comparison Of Effects Of Sleep Fragmentation On Pro-Inflammatory Cytokine Il-1Β Of Male And Female Adult Mice, Manzar Rzayeva

Mahurin Honors College Capstone Experience/Thesis Projects

Sleep plays an essential role throughout the body by affecting the physiological function and regulation of many systems. One of these systems that receives effects as a result of the adequacy of sleep is the immune system. Previous studies have demonstrated effects of sleep fragmentation upon the immune system; however, sexual differences of these effects have not been studied in depth. To analyze these variances amongst the genders, male and female adult mice were subjected to acute sleep fragmentation (SF) for 24 hours in an automated SF cage that includes a bar sweeping across the cage every two minutes. Meanwhile, …

One Cell’S Trash Is Another Cell’S Treasure: The Role Of Exosomes In Stress Physiology Of The Brown Anole (Anolis Sagrei), Anna Kaza

Honors Program Theses

Exosomes are a type of extracellular vesicle that is produced by cells through an endosomal pathway and secreted into bodily fluids. Exosomes deliver bioactive cargo from their parent cells to target cells as a form of intercellular communication. While most research has studied exosomes in the context of disease, less is known about their role in normal physiology when disease is absent. Using the brown anole (Anolis sagrei) as a model organism, we investigated how stress exposure affects exosome concentrations in the plasma of anoles, and whether exosome release is necessary for the response to stress. We hypothesized that stress …

Examining The Effects Of Exercise-Induced, Physical Stress Overtraining On Stress Biomarkers In Adolescent, C57bl/6 Mice, Curtis Scotty Davis

Theses, Dissertations and Capstones

Exercise has long been established as a therapeutic modality to improve health and related physical fitness, sports performance, and injury/risk reduction in both adults and adolescents (Haff & Triplett, 2015; Kaminsky, 2010). Nonetheless, there is a lack of research investigating the negative effects of exercise overstress (i.e., overtraining syndrome) in these populations, particularly adolescents (Brenner & American Academy of Pediatrics Council on Sports Medicine and Fitness, 2007; Matos & Winsley, 2007). The objective of this study was to examine hormone biochemical markers, anthropometric markers, and behavioral traits, which are associated with overtraining syndrome, in adolescent C57BL/6 mice. A total of …