Microbiology Commons^™

A Study Of Soil Amoebae Infective To Mice, Pamela Mcdaniel

Masters Theses

On January 1, 1973, 10 soil samples were collected from areas in Coles County, Illinois, in an effort to isolate soil amoebae. All 10 soil samples were cultured on bacto-nutrient agar and colonies of amoebae genera isolated were Acanthamoeba and Hartmannella. Amoebabacteria cultures prepared from soil isolates were injected into each of 10 adult mice which were sacrificed at the end of the nine days and examined for evidence of infection and pathogenicity. Lesions were observed in two mice and amoebae cysts and bacterial colonies were present in nine mice. Amoeba-bacteria cultures were purified using streptomycin antibiotic discs and …

The Molecular Nature And Replication Of R Factor 222 In Proteus Mirabilis, Dennis Jon Kopecko

Theses and Dissertations

The molecular nature and replicative behavior of R factor 222 was examined in Proteus mirabilis . In deoxyribonucleic acid (DNA} from R+ P. mirabilis , R factor 222 was identified by CsCl density gradient centrifugation as 2 satellite DNA bands at densities corresponding to 50 and 58 moles percent guanine plus cytosine (% GC) . Replication of the 50 and 58% GC components of R factor 222 in P. mirabilis was analyzed during growth in the presence and absence of chloramphenicol (CAM} and after shifting exponentialand stationary- phase cells to conditions which inhibit host protein or DNA synthesis . CAM …

Studies Of Teratogenic Viruses: Minute Virus Of Mice And Colorado Tick Fever Virus, Robert Eldred Harris

Theses and Dissertations

Studies were conducted in DUB/ICR mice with minute virus of mice (MVM), a single stranded DNA virus, and Colorado tick fever (CTF) virus, a double stranded RNA Virus, to determine if CTF virus had teratogenic potential and to characterize further MVM and its teratogenic effects. MVM is a known teratogen, whereas CTF virus had never been investigated previously for teratogenic potential.

In characterization studies of MVM infectivity, the following were determined: (a) Rat embryo cells proved to be superior to six cell lines in assaying for MVM infectivity; (b) MVM infectivity could be inactivated with common laboratory disinfectants, ultraviolet radiation, …

The Organ-Specific Mammalian Host-Mediated Microbial Mutagen Assay (Oshma), Roger D. Beyer

Masters Theses

No abstract provided.

An Investigation Of The Froth Flotation Process As A Means Of Concentrating Pulp And Paper Mill Sludge, Merwin Lee Miller

Masters Theses

No abstract provided.

Phytoplankton Successions And Lake Dynamics In Las Vegas Bay, Lake Mead, Nevada, Ervon R. Koening, Richard W. Tew, James E. Deacon

Publications (WR)

Phytoplankton successions, applications of the general growth equation, and physical measurements have been employed to investigate events occurring at the interface between industrial and sewage effluent contained in Las Vegas Wash and the waters of Lake Mead, Nevada. The data indicate that the entering waters tend to form a density current interrupted at intervals by dynamic effects generated in the lake. The dynamic relationships described here for the spring months suggest that a much more thorough understanding of physical, chemical and biological interactions is necessary to permit solution of the numerous problems of Las Vegas Bay discussed or alluded to …

A Mathmatical Model Of Primary Productivity And Limnological Patterns In Lake Mead, Lorne G. Everett

Publications (WR)

The temporal and spatial changes in chemical and biological properties of Lake Mead have been investigated, thereby indicating the sources of water pollution and the time of highest pollution potential. Planktonic organisms have been shown to indicate the presence of water problems. Macro- and micro-nutrient analyses have shown that primary productivity is not inhibited by limiting concentrations. A mathematical model has been developed, tested with one set of independent data, and shown worthy of management utility. Although the model works very well for the Lake Mead area, the physical reality of the Multiple Linear Regression equation should be tested on …

Phospholipid And Fatty Acid Analysis Of The Membrane Of Acetobacter Aceti Var. Liquefaciens, Cathie S. W. Kuoh

Masters Theses

No abstract provided.

Lipids Of Fungal Spores, Identification And Metabolism, Jim L. Bushnell

Theses and Dissertations

The lipids of the spores of the Basidiomycetes: Ustilago bullata, Lycoperdon perlatum and Calvatia gigantea were investigated. The spores of U. bullata were found to have hydrocarbons ranging from C_9 to C_24, methyl esters of fatty acids ranging from C_14 to C_24, and free fatty acids from C_14 to C_24. The spores of L. perlatum were found to have few hydrocarbons (C_22 being the predominent one); no methyl esters were detected and the fatty acids were from C_15 to C_18. Long chain aldehydes (C_30) were also detected in low concentration. At the ultrastructural level the Lycoperdon spores were found to …

The Effect Of Various Nutrients On The Sporulation Of Trichophyton Rubrum, P. Jerry Austin

Loma Linda University Electronic Theses, Dissertations & Projects

Trichophyton rubrum was grown on a chemically defined media in an effort to find a substance that would give the maximum production of macroconidia. Twenty-one amino acids were tested and about one half of them were active in stimulating macroconidial formation. Eight purines and pyrimidines were also tested but none of them gave more than scanty sporulation. The base media used was Czapek solution agar (modified): the saccharose was replaced with an equal amount of glucose. The various nutrients were tested at two concentrations, 1.25 mg/ml and 0.25 mg/ml. Yeast extract and peptone were also included in the study since …

The Germfree Culture Of Three Species Of Triatominae: Triatoma Protracta (Uhler), Triatoma Rubida (Uhler), And Rhodnius Prolixus Stål, Stephen A. Nyirady

Loma Linda University Electronic Theses, Dissertations & Projects

Triatoma protracta protracta, Triatoma riibida rubIda, and two strains of Rhodnius prolixus were successfully reared germfree into the fourth generation. Triatominae eggs were disinfected externally with iodine and the nymphs reared in vinyl plastic germfree modules. Germfree mice were used as the host. This is the first report of Triatominae having been reared in a germfree environment. These bugs were never exposed to bacteria internally or externally to the fourth generation.

Comparisons of conventional control bugs with replicate germfree groups in the first two generations revealed but slight differences in developmental periods. The mean adult longevity of all …

The Life Cycle Of Lissorchis Heterorchis, Macy And Krygier (1969), Brendan P. H. Onyejekwe

Dissertations and Theses

Many Flumenicola virens (Lea) collected from Crystal Springs Creek and Tualatin River, Portland, Oregon, were found to shed large numbers of a species of microcercous cercariae containing refractile granules. They resembled the cercariae of Triganodistomum mutabile (=Lissorchis mutabile Cort, 1918 as described by Wallace (1939), and subsequent experiments proved that they were the larval stages of what was described by Macy and Krygier (1969), as Lissorchis heterorchis. Encystment of these cercariae was induced experimentally in uninfected adult brown planarians collected partly from Crystal Springs Creek and partly from Carolina Biological Research Station in Gladstone, Portland, Oregon. Adult flukes …

Spatial Distribution & Temporal Occurrence Of Rotifers In The Main Pool & Tailwater Of Barren Lake, Kentucky, David Abel

Biology Graduate Theses

A study of the spatial distribution and temporal occurrence and diversity of rotifers in relation to certain chemicophysical parameters was conducted in Barren Lake, Kentucky, a flood control lake, from January, 1970, through January, 1970. Paired samples were collected from six depths in the main pool area of the reservoir and from an automatic plankton sampler installed in the tailwater area where, from April through September, the effluent was derived from the upper 3 m of the pool . Diel studies were conducted in April, July, 1970, and January, 1971.

Barren Lake began to stratify in late April, was completely …

Euryhelmis Cotti N. Sp. (Trematoda: Heterophyidae) With Observations On Its Life Cycle, Michael Joseph Simon

Dissertations and Theses

Fish of the genus Cottus were found infected with heterophyid metacercariae. Laboratory animals were infected with the metacercariae, and adult heterophid trematodes were recovered. These flukes were found to represent an undescribed species of the genus Euryhelmis. Various streams in the Willamette Valley and coastal areas were sampled for infected Cottus sp. Snails of the genera Oxytrema and Fluminicola were collected. Several possible definitive hosts were examined. A partial review of the subfamily Apophallinae and a complete review of the genus Euryhelmis are presented. Euryhelmis cotti n. sp. is placed in the subfamily Apophallinae, and its life cycle …

The Nutritional Adequacy Of Five Strains Of Bacteria For Vorticella Microstoma And Oxytricha Sp., Michael J. Sinsko

Masters Theses

No abstract provided.

Repression Of Β-Galactosidase Synthesis In Escherichia Coli By Salicylates, Joan Carlyn Olson

Dissertations and Theses

Salicylic acid, and to a lesser extent aspirin, have been shown to repress β-galactosidase synthesis in Escherichia coli. The repression is not due to decreased inducer uptake, nor does it result from competition with inducer for repressor. Dinitrophenol does not exert similar effects on β-galactosidase synthesis. Cyclic adenosine 31, 51-monophosphate partially relieves the repression. The extent or the relief by cyclic adenosine monophosphate seems to depend on the concentration of salicylic acid. This indicates there may be direct interaction between the actions of cyclic adenosine monophosphate and salicylic acid in E. coli cells.

The Role Of D-Gluconic Acid In The Regulation Of The Synthesis Of The Enzymes Of The Entner-Doudoroff Pathway In Pseudomonas Fluorescens, Steven C. Quay

Honors Theses

Induction of Entner-Duodoroff pathway enzymes in Pseudomonas fluorescens was investigated to study the role of gluconate as a possible inducer. Glucose oxidase-deficient mutants were isolated and characterized. One of these mutants, gox-7, was deficient in particulate glucose oxidase; another mutant, gox-17, was deficient in particulate glucose and gluconate oxidase activities. Gluconate, but not glucose, induced synthesis of gluconokinase and 6-phosphogluconate dehydratase in both mutants. High constitute levels of 2-keto-3-deoxy-6-phosphogluconate aldolase were found when both mutants were grown on glucose. Growth of parent and both mutant strains on glycerol also resulted in high levels of Entner-Doudoroff pathway enzymes. It was concluded …

A Pectin Histochemical Study Of The Host Pathogen Relationship Between Pyrenochaeta Terrestris And Allium Cepa, Leroy Richard Cobia

Theses and Dissertations

The involvement of pectin and pectinases in the host pathogen interaction of P. terrestris and Allium cepa were investigated by use of pectin histochemical stains at the electron microscope level. Several different pectin histochemical stains were used, but only two (the hydroxylamine reaction, and the pectin stain developed in this study) gave reliable results. It was observed that by the time the fungus has reached the host cell wall pectinesterase and endoplygalacturonase have fully removed the pectin from the host cell wall. When this has occurred, cellulases are released which continue to degrade the host cell wall. Electron transparent areas …

Histochemical Investigation On The Distribution And Localization Of Digestive Enzymes In The Alimentary Canal Of The Caddis Fly Larvae, Brachycentrus Occidentalis Banks, Walter B. Hollow

All Master's Theses

A histochemical study of the digestive enzymes of Brachycentrus occidentalis Banks showed that the insect contains the enzymes necessary to utilize the major foods, viz., carbohydrates, lipids and proteins. There appears to be a correlation between the types of digestive enzymes found and the type of food eaten by the larvae. Values of pH for extracts from the different parts of the digestive system were: foregut, 7.6-8.4, midgut, 7.6-8.4, and hindgut, 7.0-7.6. Living micro-organisms in the gut lumen was also shown.

Ultrastructural And Histochemical Investigations Of Ipomoea Batatus Lam. Infected By Rhizopus Stolonifer (Fr.) Lind, Kendall O. Smith

Theses and Dissertations

The ultrastructural aspects of Rhizopus stolonifer (Fr.) Lind. Infection in Ipomoea batatus Lam. Roots and the histochemistry of cell walls affected by enzymes secreted by the fungus were investigated. Specimens were prepared for viewing in the electron microscope by thin-sectioning techniques. Degradation of cellular membreanes and host tissues in advance of the fungus was evident. Breakdown of the middle lamella was detected by special histochemical stains for pectin. Breakdown occurred in the following was: (1) maceration of the middle lamella before maceration of the cell wall, (2) simultaneous degradation of both the middle lamella and the cell wall, (3) maceration …

The Effect Of Dimethylsulfoxide On The Infectivity Of The T1-Coliphage, Grergory Kyle Reeder

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Gregory Kyle Reeder on July 22, 1971.

Characteristics Of A Bacteriophage Isolated For Pseudomonas Tabaci, Linda R. Nichols

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Linda R. Nichols on July 19, 1971.

Effects Of Insulin, Sodium And D-Glucose On Amino Acid Absorption In The Intestine Of Rats, Andre-Gerard Craan

Dissertations and Theses

Amino acid absorption across rat intestine in vitro was inhibited or stimulated by insulin depending on the hormone serosal concentration.

The absence of sodium ions from the incubating solution resulted in a significant decrease of L-alanine absorption. However, L-alanine was enhanced in a sodium-free medium by the addition of insulin on the serosal side of the intestine.

A sizable decrease in L-alanine and L-lysine absorption was produced by introduction of D-glucose (0.2 % and 2 %) in the Krebs-Ringer's incubating buffer. Nevertheless, the presence of D-glucose did not prevent the stimulatory effect of insulin on amino acid absorption.

The Effect Of Antibiotics On Thermophilic Blue-Green Algae, Erleen Blanche Christenson

Dissertations and Theses

The nature of the action of penicillin on the cell walls of bacteria is reviewed. The composition of bacterial cell walls is compared to cell walls of blue-green algae.

The test organisms used were thermophilic: Anacystis nidulans grew optimally at 35⁰C, whereas Synechococcus lividus grew best at 45⁰ C. Growth was recorded by reading optical densities.

Cells of these two thermophilic blue-green algae were treated with varying dosages of penicillin and streptomycin. Penicillin inhibited growth of Anacystis nidulans and Synechococcus lividus in concentrations of 3.0 µg/ml and 0.03 µg/ml respectively. However, when 0.3 µg/ml of penicillin was added to Anacystis …

Bacteriuria As A Precursor Of Intracatheter Encrustation, Namcy Moll

Loma Linda University Electronic Theses, Dissertations & Projects

The problem with which this study was concerned was the development of criteria that could be used to determine when urinary catheter changes were necessary. By determining when encrustation occurred, it was felt that some complications could be eliminated by changing catheters before obstruction occurred due to encrustation.

It was the purpose of this study to: 1) explore some indications for catheter change, 2) determine if encrustation of urinary catheters necessitates catheter change, and 3) estimate potential criteria for a routine time for catheter changes.

Forty-five catheters were examined after their removal from patients. Flow rates were determined on 33 …

The Effect Of Elevated Glucose On Neutrophilic Phagocytosis In Genetically Diabetic Mice, Larry B. Thompson

Loma Linda University Electronic Theses, Dissertations & Projects

Infiltration to an injury site or area of bacterial contamination by polymorphonuclear leucocytes (or neutrophils) is an early and essential phase of the early inflammatory reaction. Their action on foreign matter is the most critical step taken by body defense mechanisms in the first 24 hours. Anything which interferes with that action, phagocytosis, greatly hampers early inflammation. This study was designed to see the effect of elevated glucose levels on neutrophil phagocytosis in a unique strain of genetically diabetic mice.

Twenty-two diabetic and twenty-four control female mice were used. Blood glucose determinations and total white counts were made on each …

Histopathological Variations Of Periapical Granulomas And Radicular Cysts, Thanapen Kanchanasut

Loma Linda University Electronic Theses, Dissertations & Projects

The periapical dental granuloma and cyst is the most frequent sequela of pulpitis. These lesions are always intraosseous and often become quiescent after the acute phases are over, forming a space which primarily consists of chronic inflammatory tissue. This chronic inflammatory tissue usually appears as a one-centimeter area of punched out bone on the x-ray film and is referred to as a dental granuloma. Should epithelium choose to proliferate in this inflammatory area, it may undergo subsequent cystic degeneration forming the typical periapical cyst. While much has been written with regard to the etiology and treatment of the apical cyst …

Properties Of Salmonella Phage P3 After Subjection To Host-Controlled Modification By Passage Through E. Coli K-12, Benjamin J. Siapco

Loma Linda University Electronic Theses, Dissertations & Projects

Five new bacteriophages were isolated from lysogenic S. potsdam in this laboratory. Group B phages (P3 and P9a) were found to have unique properties, including their capability to infect strains of E. coli K-12 and E. coli C⁺/L. Passage of the phages through these hosts modified them (host-controlled modification) so that their ability to infect their original host, S. potsdam, was reduced and only 1-4 per million were able to form plaques.

To help elucidate the biochemical basis of host-controlled modification in the Salmonella potsdam group of bacteriophages, the clear plaque mutant of one of them, P3-c …

Investigations Into The Microbial Ecology And Limnology Of Hyrum Reservoir, In Northern Utah, Kenneth Maxwell Green

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A series of preliminary investigations was carried out to determine the factors promoting the dense, late summer waterblooms of Aphanizomenon flos-aguae in Hyrum Reservoir in northern Utah. Attempts were made to culture the Aphanizomenon in the ASM-8a medium of O'Flaherty and Phinney (J. Phycol. 6:95-97. 1970), but no growth was obtained and the algae soon lysed. Cultures were maintained, without growth, for more than four months in a lake water--lake sediment medium at 17 C under 1500 lux flourescent light.

Dissolved oxygen measurements using an in situ probe revealed the development of a sharp oxycline during the summer months; on …

Peptidoglycan Differences In Strains Of Bacillus Cereus Constitutive And Inducible For Penicillinase Production, Kenneth W. Nickerson, Richard A. Day

Papers in Microbiology

Strains of Bacillus cereus differing in penicillinase production were shown to possess cell walls of differing muramic acid contents.