Biomedical Engineering and Bioengineering Commons^™

Investigation Of A Neuro-Stimulator Retrieved Posthumously, Megan Markl, Rodney J. Gutherie, Tarun Goswami

Biomedical, Industrial & Human Factors Engineering Faculty Publications

A neurostimulator was investigated in this paper posthumously. Device was presented to our anatomical gift program. Investigation was multi-fold and contained visual inspection, using an optical microscope, and mechanical and electrical testing of leads and its insulator. It was concluded that the device could have been damaged during implantation, in vivo, during removal, and/or during transportation to author’s laboratories. The damage observed on the lead insulation is similar to that which can occur due to anchoring of the lead and hardening due to oxidation. Insulation stiffness was determined to be 1/10 of new insulator. The results reported here on the …

How Degrees Of Freedom Affects Sense Of Agency, Akima Connelly, Jungsu Pak, Tian Lan, Uri Maoz

Student Scholar Symposium Abstracts and Posters

Can the rubber-hand illusion be extended to a moving robotic arm in different degrees of freedom (DOF), inducing sense of ownership & agency over the arm? We hypothesize that DOF closer to what humans possess will result in a stronger sense of ownership and agency.

Simulation Of Skull Fracture Due To Falls, Anthony Vicini, Tarun Goswami

Biomedical, Industrial & Human Factors Engineering Faculty Publications

This study presents novel predictive equations for von Mises stress values of bones in the frontal and lateral regions of the skull. The equations were developed based on results of a finite element model developed during this research. The model was validated for frontal and lateral loading conditions with input values mimetic to fall scenarios. Using neural network processing of the information derived from the model achieved R2 values of 0.9990 for both the stress and deflection. Based on the outcome of the fall victims, a threshold von Mises stress of 40.9 to 46.6 MPa was found to indicate skull …

A Multi-Scale Approach To Study Biochemical And Biophysical Aspects Of Resveratrol On Diesel Exhaust Particle-Human Primary Lung Cell Interaction, Wei Zhang, Qifei Li, Mingjie Tang, Han Zhang, Xiaoping Sun, Sige Zou, Judy L. Jensen, Theodore G. Liou, Anhong Zhou

Biological Engineering Faculty Publications

Diesel exhaust particles (DEPs) are major air pollutants that lead to numerous human disorders, especially pulmonary diseases, partly through the induction of oxidative stress. Resveratrol is a polyphenol that ameliorates the production of reactive oxygen species (ROS) and delays aging-related processes. Herein we studied the cytoprotective effect of resveratrol on DEP-exposed human lung cells in a factorial experimental design. This work investigates biophysical features including cellular compositions and biomechanical properties, which were measured at the single-cell level using confocal Raman microspectroscopy (RM) and atomic force microscopy (AFM), respectively. Principal component analysis (PCA), hierarchical cluster analysis (HCA) and partial least square …

An Algorithm For Coupling Multibranch In Vitro Experiment To Numerical Physiology Simulation For A Hybrid Cardiovascular Model, Ehsan Mirzaei, Masoud Farahmand, Ethan Kung

Publications

The hybrid cardiovascular modeling approach integrates an in vitro experiment with a computational lumped‐parameter simulation, enabling direct physical testing of medical devices in the context of closed‐loop physiology. The interface between the in vitro and computational domains is essential for properly capturing the dynamic interactions of the two. To this end, we developed an iterative algorithm capable of coupling an in vitro experiment containing multiple branches to a lumped‐parameter physiology simulation. This algorithm identifies the unique flow waveform solution for each branch of the experiment using an iterative Broyden's approach. For the purpose of algorithm testing, we first used mathematical …

Development Of Medical Devices For Osteoarthritis Diagnosis, Antimicrobial, Wound Closure, And Wound Ph Milieu Monitoring, Shuxin Li

Bioengineering Dissertations - Archive

Nanotechnology has been widely used in the field of biomedical engineering. For example, nanoparticles have been employed for targeted drug delivery due to their retention effect, increased permeability, site-specific targeting, and avoiding phagocytosis as foreign materials. Moreover, nanotools, such as liposomes, Q-dots, dendrimers, micelles, and carbon nanotubes, have been developed for disease diagnosis and treatment. Generally, the application of nanotechnology can be categorized as follows: nanomedicine, tissue regeneration, biomarker/biosensors, nanorobots, and gene therapy. In the past four years, I have been focusing on developing nanotechnology for diagnosis and treatment of many diseases such as osteoarthritis, open wound, and infection. Based …

Electropermeabilization Does Not Correlate With Plasma Membrane Lipid Oxidation, Olga Michel, Andrei G. Pakhomov, Maura Casciola, Jolanta Saczko, Julita Kulbacka, Olga N. Pakhomova

Bioelectrics Publications

The permeabilized condition of the cell membrane after electroporation can last minutes but the underlying mechanisms remain elusive. Previous studies suggest that lipid peroxidation could be responsible for the lasting leaky state of the membrane. The present study aims to link oxidation within the plasma membrane of live cells to permeabilization by electric pulses. We have introduced a method for the detection of oxidation by ratiometric fluorescence measurements of BODIPY-C11 dye using total internal reflection fluorescence (TIRF) microscopy, limiting the signal to the cell membrane. CHO-K1 cells were cultured on glass coverslips coated with an electroconductive indium tin oxide (ITO) …

Blood Glucose Predictor, Jessica Patterson

Electrical Engineering

For my senior project, I perform data analysis using statistical methods to determine body metrics that correlate with blood glucose levels. Working with Dr. Tina Smilkstein, I take repeat measurements from 6 different volunteers to establish trends in bodily metric data. The data taken includes weight, body fat, pulse rate, VO2, blood glucose, blood pressure, hours slept, and quality of sleep. Using these values, I use the program MiniTab to view results.

A few examples of correlations with blood glucose found in this project are:

• Systolic blood pressure for females had a regression line of 124.0 -0.3366*Blood Pressure. This indicates …

The Design And Development Of A Device To Assist In Boosting Patients, Taylor A. Rieckhoff

Masters Theses

A common task a nurse is required to perform is called boosting patients. Boosting a patient is defined as lifting or sliding a patient back up in the bed after having slid down (Mannheim, Zieve, & Conaway, 2017). The current method for boosting patients involves a minimum of two personnel and an 11-step process. The 11-step process requires the person to manually lift and pull the patient using an existing half sheet on the bed (Mannheim, Zieve, & Conaway, 2017). Patients who cannot move or support themselves are moved every two to six hours or upon request (Bihn, Rieckhoff, Burkman, …

Differentiating Epileptic From Psychogenic Nonepileptic Eeg Signals Using Time Frequency And Information Theoretic Measures Of Connectivity, Sarah Barnes

Masters Theses

Differentiating psychogenic nonepileptic seizures from epileptic seizures is a difficult task that requires timely recording of psychogenic events using video electroencephalography (EEG). Interpretation of video EEG to distinguish epileptic features from signal artifacts is error prone and can lead to misdiagnosis of psychogenic seizures as epileptic seizures resulting in undue stress and ineffective treatment with antiepileptic drugs. In this study, an automated surface EEG analysis was implemented to investigate differences between patients classified as having psychogenic or epileptic seizures. Surface EEG signals were grouped corresponding to the anatomical lobes of the brain (frontal, parietal, temporal, and occipital) and central coronal …

Spark Plasma Sintering Of Low Modulus Titanium-Niobium-Tantalum-Zirconium (Tntz) Alloy For Biomedical Applications, Nicholas Mavros, Taban Larimian, Javier Esqivel, Rajeev Kumar Gupta, Rodrigo Contieri, Tushar Borkar

Mechanical Engineering Faculty Publications

In metallurgy, titanium has been a staple for biomedical purposes. Its slow toxicity and alloying versatility make it an attractive choice for medical applications. However, studies have shown the difference in elastic modulus between titanium alloys (116 GPa) and human bone (10–40 GPa), which contributes to long term issues with loose hardware fixation. Additionally, long term studies have shown elements such as vanadium and aluminum, which are commonly used in Ti-6Al-4V biomedical alloys, have been linked to neurodegenerative diseases like Alzheimer and Parkinson. Alternative metals known to be less toxic are being explored as replacements for alloying elements in titanium …

Optical Spectrsocopy Of Murine Breast Tumor To Distinguish Indolent From Aggressive Disease, Joel Rodriguez Troncoso

Biomedical Engineering Undergraduate Honors Theses

Breast cancer accounts for 30% of all cancer. Metastasis is the primary cause of death among breast cancer patients. Additionally, current molecular profiling methods such as Oncotype DX, which are expensive and not widely available at all clinical facilities, only determine the risk of recurrence after treatment. Therefore, there are no current method capable of identifying metastatic patients in advance.As a result, there is an unmet clinical need to develop a cost-effective prognostic to differentiate between indolent and aggressive breast tumors. In this study, we implemented diffuse reflectance spectroscopy (DRS) system to evaluate functional changes in tumor xenografts originated from …

Expression Systems For Synthetic Spider Silk Protein Production, Michaela R. Hugie

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Spider silk is a biodegradable and biocompatible natural material that is stronger than steel and more elastic than nylon. These properties make spider silk a desirable material for many commercial products, ranging from textiles to biomedical materials. Due to spiders’ cannibalistic and territorial nature it is impossible to farm them to produce spider silk at a high enough yield to meet product demands. Therefore, a bioengineered synthetic process is necessary to produce spider silk. Synthetic spider silk has been produced in bacteria, goats, yeast, plants, mammalian cells and silkworms, but none of these processes provided a commercially viable yield or …

Controlling Strain Energy Density In 3d Cellular Collagen Constructs During Complex Loads, Katherine Hollar

Boise State University Theses and Dissertations

Mechanical stimulation applied to damaged soft tissues, such as ligament, can promote tissue remodeling to accelerate healing. To help identify treatments that encourage ligament healing, bioreactors have been designed to subject 3D cellularized constructs to various loading conditions in order to determine the mechanical mechanisms that trigger cell-mediated repair. An innovative approach is to use a bioreactor to apply controlled states of biaxial stress to study the effects of strain energy density and distortion energy on cell activity. Tissue distortion has been linked to changes in the structure and function of ligament, yet the specific impact of distortion energy on …

Effect Of Corrective Surgery On Lower Limb Mechanics In Patients With Crouch Gait, Adelle Milholland

Boise State University Theses and Dissertations

Crouch gait a progressively degrading gait deviation associated with the neurological disorder cerebral palsy. If left untreated it can lead to anterior knee pain and a loss of ambulation. At present there exists no agreed upon metric for determining the surgical procedures used to treat crouch gait and there is insufficient means to analytically compare the results of different procedures. The aims of this thesis work were to create a pipeline to transform a patient’s gait analysis data into a finite element model, develop a model of sufficient complexity to evaluate a range of outcomes by which to judge the …

Single-Cell Impedance Spectroscopy, David Paul Lange

Master's Theses

Impedance spectroscopy (IS) is an important tool for cell detection and characterization in medical and food safety applications. In this thesis, the Cal Poly Biofluidics Lab’s impedance spectroscopy system was re-evaluated and optimized for single-cell impedance spectroscopy. To evaluate the IS system, an impedance spectroscopy bioMEMS chip was fabricated in the Cal Poly Microfabcrication lab, software was developed to run IS experiments, and studies were run to validate the system. To explore IS optimization, Maxwell’s mixture theorem and the Schwartz-Christoffel transform were used to calculate an analytic impedance solution to the co-planar electrode system,a novel volume fraction to account for …

Development Of A Nucleus Pulposus Implant For Intervertebral Disc Repair, Joshua Dillon Walters

All Dissertations

The intervertebral disc (IVD) is a fibrocartilaginous tissue connecting adjacent vertebrae in the spinal column. It comprises three distinct tissues: a gelatinous core known as the nucleus pulposus (NP), concentric fibrous rings encircling the NP known as the annulus fibrosus (AF), and the superior/inferior cartilaginous endplates (CEPs). The complex mechanical interplay of these tissues allows the IVD to withstand complex loading in the spine while maintaining trunk stability and flexibility. IVD pathologies, such as IVD degeneration (IDD) and herniation, are associated with cell-mediated inflammation in vivo. This inflammation creates a catabolic environment which degrades the extracellular matrix (ECM) of the …

Production And Purification Of Basic Fibroblast Growth Factor Fused To Two Collagen Binding Domains Expressed In E. Coli Bl21 Using Flask And Fed-Batch, Hazim Aljewari

Graduate Theses and Dissertations

Delivering effective and non-toxic doses of bioactive materials that can aid in activating tissue regeneration to wounded tissue has proven to be an enormous challenge. This study was designed to produce a potential therapeutic recombinant protein by fusing two collagen binding domains to basic fibroblast growth factors (bFGF) through a collagenase cleavage site linker, so it can release the bFGF in a wound site by the action of this enzyme. The novel fusion protein was expressed in Escherichia coli BL-21 (E. coli) using traditional flask shaker and fed-batch cultivation. Cell lysate was purified by FPLC using Immobilized metal affinity chromatography …

Method For Spatial Overlap Estimation Of Electroencephalography And Functional Magnetic Resonance Imaging Responses, N. Heugel, E. Liebenthal, Scott A. Beardsley

Biomedical Engineering Faculty Research and Publications

Background

Simultaneous functional magnetic resonance imaging (fMRI) and electroencephalography (EEG) measurements may represent activity from partially divergent neural sources, but this factor is seldom modeled in fMRI-EEG data integration.

New method

This paper proposes an approach to estimate the spatial overlap between sources of activity measured simultaneously with fMRI and EEG. Following the extraction of task-related activity, the key steps include, 1) distributed source reconstruction of the task-related ERP activity (ERP source model), 2) transformation of fMRI activity to the ERP spatial scale by forward modelling of the scalp potential field distribution and backward source reconstruction (fMRI source simulation) …

An Evaluation Of The Host Response To An Interspinous Process Device Based On A Series Of Spine Explants: Device For Intervertebral Assisted Motion (Diam®), Jeffrey M. Toth, Justin D. Bric

Biomedical Engineering Faculty Research and Publications

Background:

The objective of this study was to evaluate the host response to an interspinous process device [Device for Intervertebral Assisted Motion (DIAM®)] based on a series of nine spine explants with a mean post-operative explant time of 35 months.

Methods:

Explanted periprosthetic tissues were processed for histology and stained with H&E, Wright-Giemsa stain, and Oil Red O. Brightfield and polarized light microscopy were used to evaluate the host response to the device and the resultant particulate debris. The host response was graded per ASTM F981-04. Quantitative histomorphometry was used to characterize particle size, shape, and area per ASTM F1877-05. …

Vastly Extended Drug Release From Poly(Pro-17Β-Estradiol) Materials Facilitates In Vitro Neurotrophism And Neuroprotection, Anthony R. D'Amato, Devan L. Puhl, Samuel A.T. Ellman, Bailey Balouch, Ryan J. Gilbert, Edmund F. Palermo

Chemical and Biochemical Engineering Faculty Research & Creative Works

Central nervous system (CNS) injuries persist for years, and currently there are no therapeutics that can address the complex injury cascade that develops over this timescale. 17β-estradiol (E2) has broad tropism within the CNS, targeting and inducing beneficial phenotypic changes in myriad cells following injury. To address the unmet need for vastly prolonged E2 release, we report first-generation poly(pro-E2) biomaterial scaffolds that release E2 at nanomolar concentrations over the course of 1–10 years via slow hydrolysis in vitro. As a result of their finely tuned properties, these scaffolds demonstrate the ability to promote and guide neurite extension ex vivo and …

Quantitative Analysis Techniques For Assessing Organelle Organization And Dynamics In Individual Cells, Isaac Vargas

Graduate Theses and Dissertations

In biomedical optics and microscopy, the organization and morphology of organelles have been widely studied. In spite of novel imaging techniques, there is still a lack of quantitative tools to easily measure cellular characteristics from image data. Previous studies have explored multiple approaches to assess organelle organization and alignment, resulting in complicated and extensive algorithms that are both subject to multiple steps of image processing and influenced by non-cellular artifacts. In this thesis, a technique called the Modified Blanket Method (MBM) is introduced to quantify organelle organization through measurements of fractal dimension (FD) on a pixel-by-pixel basis. With the use …

In Vivo Metabolic And Vascular Response To Hypoxia In Twist Knockdown Murine Breast Cancer, Brandon Sturgill

Graduate Theses and Dissertations

Twist transcription factor is often overexpressed in aggressive tumors. Although needed in early embryonic development for organogenesis, Twist is known to induce an epithelial to mesenchymal transition in cells. In cancer, epithelial to mesenchymal transitions can lead to increased motility and invasiveness. It has also been linked to metabolic reprogramming and increased metastatic risk. Furthermore, metabolic preferences can increase proliferation, enhance metastatic potential, and influence the site of metastasis. We hypothesize that Twist directly affects the metabolism of cancer cells. We expect to see in vivo what we have seen in vitro; Twist overexpression should promote a shift away from …

Optimized Production And Evaluation Of Cellulose Nanocrystals Derived From Pre-Extracted Kraft Pulp Of Different Wood Species, Gurshagan Kandhola

Graduate Theses and Dissertations

Production of nanocellulose from a variety of naturally abundant, locally available and industrially significant wood species provides an opportunity for diversifying the portfolio of traditional pulp and paper industries. The U.S. has a prolific forest products industry with a well-established infrastructure that could be utilized for optimized and customized production of cellulose nanomaterials. However, to achieve that, it is important to a) understand how biorefining strategies for complete fractionation of biomass affect the downstream processing of pulp into nanocellulose, b) maximize the yields of cellulose nanocrystals and nanofibers (CNCs and CNFs) from pretreated raw materials, and c) evaluate if the …

Development Of A Novel System For The Analysis Of The Cardiovascular And Autonomic Nervous Systems, Seyedmohsen Dehghanojamahalleh

Theses and Dissertations

Chapter 1 - An introduction to autonomic nervous system (ANS) and cardiovascular system (CVS): The performance of the ANS can be evaluated by quantizing the changes made by the autonomic nervous system. The most common parameters that are being used in autonomic nervous system assessment tests are blood pressure, heart rate, pressure pulse wave velocity, heart rate variability, and pulse waveform. In this dissertation, we designed and developed a system that assesses the activity of these two systems. Therefore, we represented four methods to 1- evaluate the dependence of neuro- and cardiovascular systems on sex, 2- non-invasively estimate the blood …

Microwave-Assisted And One-Step Synthesis Of Peg Passivated Fluorescent Carbon Dots From Gelatin As An Efficient Nanocarrier For Methotrexate Delivery, Nasser Arsalani, Parinaz Nezhad-Mokhtari, Esmaiel Jabbari

Faculty Publications

A green and simple process for preparing the polyethylene glycol passivated fluorescent carbon dots (CDs-PEG) have been studied by a microwave pyrolysis method, using gelatin and PEG as starting materials. This method is very effective for development of carbon-based quantum dots from gelatin with high quantum yield (QY). The synthesized CDs-PEG were found to emit blue photoluminescence (PL) with a maximum QY of 34%. At the following research, we investigated the effect of the presence of PEG on PL intensity, and the result showed that CDs-PEG becomes stronger PL properties than pure CDs from gelatin. The synthesized CDs-PEG were characterized …

Deep Brain Stimulation: Prediction Model For Volume Of Tissue Stimulated, Melissa Jones, Tarun Goswami

Biomedical, Industrial & Human Factors Engineering Faculty Publications

An attempt has been made to understand deep brain stimulation in humans via marketable devices that are approved by the US Food and Drug Administration. The electrode characteristics were presented to influence the volume of tissue stimulated. Experimental data were digitized and VTS data were presented a function of electrode diameter, aspect ratio, applied current, pulse duration, voltage and frequency. Amongst other trends voltage and VTS were found to exhibit a three-stage relation, where Stage II characteristics were represented by a linear equation. It is in this stage, the VTS was found to be stable and where the most effective …

Effects Of Cryopreservation And Next Generation Sequencing Of Adipose Derived Stem Cells, Mulla Shahensha Shaik

LSU Doctoral Dissertations

The research in this thesis is divided into two areas. 1) Studying the effects of cryopreservation and developing freezing protocols suitable for adipose derived stem cells and 2) Identifying the underlying mechanisms involved in the differentiation of ASCs especially into osteogenesis and endothelial-genesis using next generation sequencing.

In the part one (Chapter 2 and 3), a novel protocol to freeze ASCs and the effects of long-term cryopreservation on ASCs were studied. Heat shocking ASCs at 43°C for 1 hour resulted in the temporal expression of HSPs particularly HSP70 and HSP32 to reach maximum expression at 3 hours post heat shock …

Fluid Shift And Fluid Resuscitation In Burn Patients With The Use Of Bio-Electrical Impedance Spectroscopy To Monitor Fluid Levels, Temitope D. Obielodan

Honors College Theses

The purpose of this research is to explore the current methods of fluid resuscitation and other possible methods of measuring the body fluid levels of burn patients in order to fully understand the fluid increase patterns in the torso area. This will be done primarily by focusing on the concept of bio-electrical impedance spectroscopy to measure the fluid levels only in the human torso area. Three similar tests were carried out by measuring the resistance values after ingesting 500ml of water. This was repeated until a total of 1500ml of water was ingested. It was found that the resistance in …

How Unstructured Data From The Data Warehouse Can Be Used With Machine Learning And Visualization To Develop Novel Medical Technologies, Alfred A. Cecchetti

Faculty Research Day

Machine Learning (ML) can accurately classify and accurately predict disease as well as other medical events.

Device Programming, especially smartphone applications, can provide new ways to acquire, transport, store, process, and secure personalized patient data to deliver meaningful results.