Open Access. Powered by Scholars. Published by Universities.®
Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Molecular, Cellular, and Tissue Engineering (46)
- Life Sciences (44)
- Biological Engineering (41)
- Biomedical Devices and Instrumentation (32)
- Medicine and Health Sciences (20)
-
- Cell and Developmental Biology (18)
- Physical Sciences and Mathematics (17)
- Biomechanics and Biotransport (15)
- Electrical and Computer Engineering (15)
- Bioimaging and Biomedical Optics (14)
- Cell Biology (14)
- Chemical Engineering (13)
- Biomaterials (12)
- Bioresource and Agricultural Engineering (11)
- Biochemistry, Biophysics, and Structural Biology (10)
- Environmental Sciences (10)
- Biochemical and Biomolecular Engineering (8)
- Bioelectrical and Neuroengineering (7)
- Biomedical (7)
- Materials Science and Engineering (7)
- Nanoscience and Nanotechnology (7)
- Systems and Integrative Engineering (7)
- Cancer Biology (6)
- Diseases (6)
- Food Science (6)
- Microbiology (6)
- Polymer and Organic Materials (6)
- Biochemistry (5)
- Keyword
-
- Applied sciences (28)
- Biological sciences (11)
- Biosensor (5)
- Breast Cancer (5)
- Metabolism (5)
-
- Tissue engineering (5)
- Biomaterials (4)
- Cancer (4)
- Diffuse reflectance spectroscopy (4)
- Scaffold (4)
- Aptamer (3)
- Biomechanics (3)
- Breast cancer (3)
- Cytokines (3)
- Earth sciences (3)
- Extracellular Matrix (3)
- Extracellular matrix (3)
- Nanocellulose (3)
- Pure sciences (3)
- Tissue Engineering (3)
- Volumetric muscle loss (3)
- Azoxymethane (2)
- Biochemistry (2)
- Bioenergy (2)
- Bioengineering (2)
- Biomedical imaging (2)
- Cellulose Nanocrystals (2)
- Decellularization (2)
- Deep learning (2)
- Dilute acid pretreatment (2)
Articles 1 - 30 of 133
Full-Text Articles in Biomedical Engineering and Bioengineering
Design, Fabrication, And Testing Of A Pdms-Based Nanochannel Using Nano Embossing, Cooper James Haskamp
Design, Fabrication, And Testing Of A Pdms-Based Nanochannel Using Nano Embossing, Cooper James Haskamp
Graduate Theses and Dissertations
DNA sequencing has become increasingly important in the field of biomedical research and clinical applications. Currently, the most well-established sequencing technique is ‘Sequencing by Synthesis’ with accuracy in the range of 99.2% to 99.74% and sequencing lengths of only 300 base pairs. Sequencing by Synthesis is a lab-based method that requires expensive equipment and fast parallel computing. It is not suitable for portable, on-site applications. A new technique known as ‘nanopore’ was developed to meet these requirements. Nanopore is an electrical sequencing method that relies on the translocation of a DNA molecule through a nanoscale pore, where the blockage current …
Extending Touch: Investigating Sensorimotor Integration, Cognitive Workload, And Presence With Neuro-Haptic Feedback In Virtual Reality, Aliyah Khadijah Shell
Extending Touch: Investigating Sensorimotor Integration, Cognitive Workload, And Presence With Neuro-Haptic Feedback In Virtual Reality, Aliyah Khadijah Shell
Graduate Theses and Dissertations
Sensory feedback from the hand is fundamental for the coordination of fine motor skills and overall sensorimotor function. Impaired somatosensation during task execution has been associated with the development of inaccurate motor plans, which can hinder motor learning. Virtual Reality (VR) has emerged as a promising tool for motor rehabilitation; however, most VR-based interventions overlook the critical role of tactile feedback in motor control. The absence of touch feedback, which is integral to conventional rehabilitation and training, may limit the full therapeutic potential of VR. Integrating functionally relevant neurostimulation based haptic feedback (neuro-haptic feedback) into VR-based interventions may enhance functional …
Tissue Engineered Combinatorial Therapeutics For Spinal Cord Injury Repair, Inha Baek
Tissue Engineered Combinatorial Therapeutics For Spinal Cord Injury Repair, Inha Baek
Graduate Theses and Dissertations
Traumatic spinal cord injury (SCI) lead to temporary or permanent sensorimotor deficit due to the complex and multifaceted features of the injury site, making the treatments ineffective. Recent research emphasizes the potential of combinatorial therapeutics approaches combining different therapeutics, such as biomaterials and stem cell transplantation. In this context, nerve composite hydrogels, fabricated from decellularized sciatic nerve (dSN) and spinal cord (dSC) extracellular matrices (ECM), might be promising platforms due to their biocompatibility and ability to mimic native microenvironments. In this study, we developed and characterized nerve mimetic composite hydrogels embedded with human adipose-derived stem cells (hASCs) and investigated their …
In Vitro Investigation Of Macrophage Functional And Metabolic Modulation, Shelby Nicole Bess
In Vitro Investigation Of Macrophage Functional And Metabolic Modulation, Shelby Nicole Bess
Graduate Theses and Dissertations
Macrophages are a subset of immune cells with a variety of functions that are abundant in numerous tumor types such as colorectal and breast cancer. It has been suggested that macrophages may serve an important function as immune mediators in tumor behavior; however, macrophage activity is complex and remains not completely understood. The overall goal of this dissertation was to explore and exploit tumor-associated macrophage phagocytic function and the changes in their interactions with cancer cells in an in vitro mouse model and in 2D and 3D in vitro culture models. First, we wanted to better understand macrophage behavior under …
Using Deep Learning And Two-Photon Excitation Fluorescence Microscopy To Predict Breast Cancer Recurrence And Response To Chemotherapy, Nicholas Powell
Using Deep Learning And Two-Photon Excitation Fluorescence Microscopy To Predict Breast Cancer Recurrence And Response To Chemotherapy, Nicholas Powell
Graduate Theses and Dissertations
Accurate prediction of breast cancer recurrence plays a critical role in guiding treatment decisions, particularly regarding the use of systemic therapy for patients. While genomic assays such as the Oncotype DX Recurrence Score offer valuable prognostic information, they are limited in accessibility and application. The work presented in this thesis explores an imaging-based approach for the prediction of breast cancer recurrence that combines multiphoton microscopy (MPM) with deep learning to classify individual breast cancer biopsy samples by risk of recurrence. Genomic differences in tumors with different recurrence potentials may translate to distinct optical and structural information that can be captured …
Multimodal Spectroscopy To Study Tumor Microenvironmental Changes In Vivo, April Mordi
Multimodal Spectroscopy To Study Tumor Microenvironmental Changes In Vivo, April Mordi
Graduate Theses and Dissertations
Every year there are millions of new cancer diagnoses and deaths worldwide. While great strides have been made in technologies and drugs for cancer treatment, there is still a need to understand tumor response to therapy as many patients still endure treatment failure after completing their treatment regimen. Current clinical imaging techniques for detecting response to therapy are utilized weeks to months after the course of treatment, and present limitations to measuring response during treatment. Optical spectroscopy techniques can help meet this need to determine the response of tumors to treatment as it allows for near real-time in vivo characterization …
Synthesis And Process Optimization Of Cellulose Nanocrystals From Miscanthus X. Giganteus For Commercial Viability, Jaspreet Kaur
Synthesis And Process Optimization Of Cellulose Nanocrystals From Miscanthus X. Giganteus For Commercial Viability, Jaspreet Kaur
Graduate Theses and Dissertations
Cellulose nanocrystals (CNCs), derivatives of an abundant biopolymer, i.e., cellulose, are emerging as promising alternatives to petrochemical-based products due to their unique properties, including biodegradability, low toxicity, high strength, low density, large surface area and low coefficient of expansion. CNCs have diverse applications ranging from food packaging and textiles to drug delivery and medical implants, etc. Despite their potential, the widespread commercialization of CNCs faces significant hurdles owing to the high environmental burden and production costs. They are mainly driven by the commonly used source utilized for CNC synthesis i.e., wood and the intensive synthesis process i.e., requirement of …
Two-Photon Imaging For Characterizing Tumor Microenvironmental Changes In Non-Small Cell Lung Cancer Associated With Metastatic Recurrence, Paola Monterroso Diaz
Two-Photon Imaging For Characterizing Tumor Microenvironmental Changes In Non-Small Cell Lung Cancer Associated With Metastatic Recurrence, Paola Monterroso Diaz
Graduate Theses and Dissertations
Lung cancer remains the leading cause of cancer deaths, comprising nearly 25% of all cancer deaths (Sung et al., 2021a). The five-year survival rate of patients with non-small cell lung carcinoma (NSCLC) remains significantly low given that over half present with locally advanced or metastatic disease at time of diagnosis, and experience tumor recurrence following therapeutic intervention (Tamura et al., 2015; Uramoto & Tanaka, 2014). Current evaluation techniques to assess treatment response are lacking, given they are implemented several weeks after treatment completion and are solely based on anatomical changes in tumor size, forgoing other criteria such as functional or …
Tissue Engineered Models Of Environmental And Metabolism-Driven Cancer-Nerve Crosstalk, Emory Gregory
Tissue Engineered Models Of Environmental And Metabolism-Driven Cancer-Nerve Crosstalk, Emory Gregory
Graduate Theses and Dissertations
Purpose: Within the last 30 years, cancer-nerve interactions have garnered attention for causing patient pain, increasing the risk of recurrence, and decreasing patient prognosis. There are 2 key forms cancer-nerve crosstalk takes: perineural invasion (PNI), in which cancer cells infiltrate nerves as a route for metastasis, and tumor innervation (TI), in which neurites penetrate solid tumors to promote tumor progression. Present in vitro models of cancer-nerve crosstalk primarily include Matrigel® mouse matrix and collagen I hydrogels; whereas collagen I hydrogels neglect tumor extracellular matrix (ECM) complexity, Matrigel® is controversial for its batch-to-batch inconsistencies and inclusion of experiment-altering growth factors. Decellularized …
Development Of A High-Throughput Screening Platform To Profile Gene Delivery And Transcriptional Control In Macrophages, Alexis Ivy
Graduate Theses and Dissertations
Macrophages are an important cell type in the body responsible for orchestrating the inflammatory response and return to homeostasis. Macrophages are often implicated in a multitude of disease pathologies including delayed wound healing, excessive fibrosis, autoimmune disease, and immunosuppression within the tumor microenvironment. Although macrophages have been targeted for therapeutic applications, success in utilizing them has remained elusive due to their relative ease of phenotype switching between inflammatory and anti-inflammatory. The challenge that remains is developing clinically relevant delivery vehicles that can target macrophages without causing a phenotypic switch counter-productive to the desired response. This is especially important when targeting …
Feasibility Study For The Application Of A Solar Powered Absorption Chilling System For Cooling Poultry Houses In Northwest Arkansas, Matthew James Kelly
Feasibility Study For The Application Of A Solar Powered Absorption Chilling System For Cooling Poultry Houses In Northwest Arkansas, Matthew James Kelly
Graduate Theses and Dissertations
This paper investigates the economic feasibility of utilizing a solar-powered absorption chiller to provide cooling for poultry houses in northwest Arkansas. An existing thermodynamic model of a poultry house was modified to account for cooling. A simulation was performed using actual weather data from Fayetteville, Arkansas for 26 flocks of broilers at four cooling capacities for the 2020, 2021, and 2022 calendar years. Based on the simulation, the savings from reduced electricity use for ventilation and the cost of the required absorption chiller and solar thermal array for an 8-house farm were calculated. While providing cooling was found to reduce …
Optimization Of And Mechanistic Insights Into Photocatalytic And Non-Thermal Plasma Degradation Of Organic Pollutants For Sustainable Wastewater Treatment, Yiting Xiao
Graduate Theses and Dissertations
Water pollution by organic pollutants poses a significant environmental threat, necessitating the development of advanced treatment technologies to ensure sustainable and efficient remediation. This dissertation explored innovative approaches to enhance the degradation of organic pollutants through the integration of photocatalytic and plasma-assisted systems, with a focus on optimizing these processes using methodologies ranging from response surface methodology (RSM) to advanced machine learning techniques. The first part of the dissertation investigated the degradation of organic pollutants in flocculated liquid digestate using photocatalytic titanate nanofibers (TNFs) synthesized via a hydrothermal method. This study represents the first application of TNFs, with a bandgap …
A Framework Of Flow Models For The Development And Testing Of Cardiovascular Devices, Megan Laughlin
A Framework Of Flow Models For The Development And Testing Of Cardiovascular Devices, Megan Laughlin
Graduate Theses and Dissertations
Cardiovascular disease (CVD) is the leading cause of death worldwide and its treatment continues to improve due to the advancement of innovative cardiovascular medical devices. In vitro and in silico models serve to replicate the in vivo environment for the development and testing of these devices prior to using animals for testing, and eventually humans in clinical trials. However, limitations related to these models exist in multiple areas including general, cardiac, and venous applications. Specifically, current gaps have been identified with customizable, tissue-mimicking phantoms (general), 2D Blood Speckle Imaging (BSI) (cardiac) and a bioprosthetic venous valve (venous). This dissertation focuses …
Tissue-Engineered Models For Investigating Nerve Infiltration In Fibrotic Microenvironments, Nikolas Ala-Kokko
Tissue-Engineered Models For Investigating Nerve Infiltration In Fibrotic Microenvironments, Nikolas Ala-Kokko
Graduate Theses and Dissertations
Neuron infiltration is involved in a multitude of diseased states and is critical in the regeneration of physiologic conditions. Modeling of innervation in three-dimensional, heterogenous, environments would allow early investigation in the crosstalk of neurons and native cells in normal and pathophysiologic microenvironments to gain an understanding of mechanistic functions. This would allow for greater insight into the role of metabolism, cell adhesion, microenvironmental alternations, cellular crosstalk, and signaling pathways that play a role in the promotion and prevention of neurite growth. In this thesis, with the construction of collagen-based hydrogels as the foundation for cell culture, we investigate cell …
Injectable Immunomodulatory Strategies To Enhance Muscle Recovery Following Injury, Tai Huu Huynh
Injectable Immunomodulatory Strategies To Enhance Muscle Recovery Following Injury, Tai Huu Huynh
Graduate Theses and Dissertations
Although skeletal muscle displays an astonishing regenerative capacity, injuries or diseases that resulted in bedridden or chronic muscle wasting can overwhelm this intrinsic feature of skeletal muscle and lead to functional deficit (range of motion and/or strength) and overall reduction in quality of life. Microenvironmental cues within injured skeletal muscle dictate regenerative and repair process which are tightly coordinated interplay among resident cells, cells recruitment and immune response following an assault in the muscle extracellular matrix (ECM). The successful regeneration of functional tissues requires both appropriate modulation of the inflammatory response, and activation of a variety of cell populations. Biomaterials …
Bio-Methane Production From Anaerobic Co-Digestion Of Poultry Litter With Wheat Straw Improved By Process Optimization And Employment Of Advanced Techniques, Yuanhang Zhan
Graduate Theses and Dissertations
Anaerobic co-digestion (Co-AD) of poultry litter (PL) and wheat straw (WS) for sustainable waste management and simultaneously renewable bioenergy generation was improved by advanced technologies including micro-aeration (MA), biochar addition (BC), nanoparticles supplementation (NP) and their integrations. Response surface methodology (RSM) and artificial neural network (ANN) were used for the process modeling and optimization. First, a method by gradually increasing OLR was developed for the start-up of Co-AD of PL and WS in an anaerobic sequencing batch reactor (ASBR), with a continuous daily biogas production of (13.06 ± 0.21) L and methane content (MC) of (54.38 ± 0.53) % observed. …
Polymeric Biomaterials Approaches For Engineering The In Vitro Cellular Microenvironment For Mscs, Mahsa Letter-Mahsa Haseli
Polymeric Biomaterials Approaches For Engineering The In Vitro Cellular Microenvironment For Mscs, Mahsa Letter-Mahsa Haseli
Graduate Theses and Dissertations
Cell therapy is a technology that relies on replacing diseased or dysfunctional cells with healthy functioning ones. One of the cells used for such advanced therapies are stem cells, owing to their ability to differentiate into specific cells required for repairing damaged or defective tissues or cells. The majority of cell-based products are intended to transiently persist in the patient, secreting factors which then allow the patient’s body to heal; in these products, the cells are subsequently eliminated from the body. Furthermore, unique manufacturing platforms, in addition to novel commercialization strategies, will be required to create a successful, sustainable cell …
The Influence Of Pesticides On Us Corn Production Environmental Impact: A Comparative Life Cycle Assessment Of Dominant Production Practices, Kyle Marinoni Lawrence
The Influence Of Pesticides On Us Corn Production Environmental Impact: A Comparative Life Cycle Assessment Of Dominant Production Practices, Kyle Marinoni Lawrence
Graduate Theses and Dissertations
In recent years there has been increasing interest in reducing agricultural environmental footprints in the United States, particularly for commodity crops like corn grain. Pest management is one aspect of agricultural production that is often identified as having potential for improvement with regard to environmental impact, but there is not a robust body of literature to identify the wide-scale influence of pesticides on yield and environmental impact. In this study, we hypothesized that the use of different classifications of pesticides has a significant impact on the overall environmental impact. To test this, agricultural models were created to represent the dominant …
Dpd Guided Insight On The Formation Process Of Polyethersulfone Membranes By Nonsolvent Induced Phase Separation And The Effects Of Additives, Eric Ledieu
Graduate Theses and Dissertations
Dissipative particle dynamics (DPD), a coarse grain simulation method, was applied to the membrane formation process of non-solvent induced phase separation (NIPS) to gain further insight on the mechanism of certain variables and how they affect the final morphology. NIPS involves two solutions, an organic polymer dissolved in an organic solvent colloquially called the dope and an aqueous coagulation bath, brought into contact with one another. The solvents then mix, causing the polymer to fall out of solution as an asymmetric membrane with a dense surface layer and a more open subsurface layer in response to the decreasing solubility. Polyethersulfone …
Effect Of Human Decellularized Skeletal Muscle On Recovery From Volumetric Muscle Loss Injury, Jacob Schluns
Effect Of Human Decellularized Skeletal Muscle On Recovery From Volumetric Muscle Loss Injury, Jacob Schluns
Graduate Theses and Dissertations
Volumetric muscle loss (VML) overwhelms muscle’s robust capacity for regeneration. A key event in the etiology of VML injury is the bulk loss of structural cues provided by the underlying extracellular matrix (ECM). While muscle is a highly structured tissue, with cell and ECM alignment in the direction of contractile force production, the impact of scaffold alignment on recovery remains unclear. Bulk human decellularized skeletal muscle (DSM) tissues were sectioned into 10 x 1-2 mm fibers. VML defects were repaired using multi-fiber implants consisting of approximately 8 fibers per defect arranged into two layers. Fibers were oriented 1) to the …
Automation Of Life Cycle Assessment, Jacob Hickman
Automation Of Life Cycle Assessment, Jacob Hickman
Graduate Theses and Dissertations
An automation program, named Jacob LCA, was created to help perform life cycle assessment (LCA). The program uses a template file to perform controlled and consistently ordered actions with the LCA program, SimaPro, and effectively removes the need for manual user input. It can be set to run using data from one or more life cycle inventory (LCI) files, which allows for rapid iteration and testing of data. It also partially addresses some of the limitations of LCA by establishing a procedure through which temporal and spatial variations in data can be integrated into LCI files and then passed to …
Mitral Valve Imaging And Biomechanics: A Workflow Towards Computational Modeling And Validation, Sam Stephens
Mitral Valve Imaging And Biomechanics: A Workflow Towards Computational Modeling And Validation, Sam Stephens
Graduate Theses and Dissertations
The mitral valve serves a critical role in healthy cardiac function by ensuring the unidirectional flow of oxygenated blood from the left atrium into the left ventricle. It experiences the highest pressures found within the heart and its closure is the result of a complex interaction of several different structures that, furthermore, are unique to each individual. Despite the valve’s vital role however, the specific function of these constituent structures is not fully understood. This, confounded by its asymmetric, personalized nature, make surgical interventions for the mitral valve far less effective than for its neighboring aortic valve. Efforts to overcome …
Development Of Medical Device For Volume Status Assessment Using Peripheral Venous Pressure, Audra Beneux
Development Of Medical Device For Volume Status Assessment Using Peripheral Venous Pressure, Audra Beneux
Graduate Theses and Dissertations
The severe side effects of acute dehydration and blood loss may be prevented if assessed and treated quickly. As amplifier technology has improved, small peripheral venous pressure (PVP) signals collected using a transducer on an intravenous catheter can be analyzed to monitor patient volume status. This thesis details the development of an embedded system prototype that quickly and accurately assesses volume status using PVP analysis. The volume prediction algorithm classified 10 seconds of PVP data as “dehydrated” or “resuscitated”. The hardware design consisted of four primary areas: analog signal conditioning, processing, user interaction, and power. After data collection, the signal …
Engineer And Test A Biodegradable Microneedle Patch To Deliver Meloxicam For Managing Pain In Cattle, Katherine Alejandra Miranda Munoz
Engineer And Test A Biodegradable Microneedle Patch To Deliver Meloxicam For Managing Pain In Cattle, Katherine Alejandra Miranda Munoz
Graduate Theses and Dissertations
Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin. In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested. The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated. In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis. In-vivo studies were performed to analyze the dissolution capability of …
Enhancing Human Schwann Cells Reparative Behavior Using Heparin/Collagen Layer-By-Layer Coatings, Luis Carlos Pinzon-Herrera
Enhancing Human Schwann Cells Reparative Behavior Using Heparin/Collagen Layer-By-Layer Coatings, Luis Carlos Pinzon-Herrera
Graduate Theses and Dissertations
When a peripheral nerve injury (PNI) occurs, the gold standard for tissue regeneration is the use of autografts. However, due to the secondary effects produced by multiple surgeries involved in the removal and implantation of autografts for very small lesions, it is possible to replace them with the use of Nerve Guide Conduits (NGCs). However, NGCs are limited to short lesions (less than 1 cm). This limitation is caused by the absence of compounds in the extracellular matrix (ECM) that autografts can provide. Since much of the regenerative process takes place on the NGC surface, our work aims to modify …
Ultrasound Mediated Tissue Growth In Bioinspired Scaffolds, Marco Fielder
Ultrasound Mediated Tissue Growth In Bioinspired Scaffolds, Marco Fielder
Graduate Theses and Dissertations
Porous scaffolds mechanically stimulated with ultrasound are used for the treatment of bone conditions such as osteoporosis or traumatic bone injury. However, one current issue in these treatment techniques is designing a scaffold structure with a high structural integrity that also does not impede tissue growth. A larger ultrasound wave transmission would also allow more of a scaffold to receive mechanical stimulation from ultrasound. Controlling mesenchymal stem cell seeding of tissue scaffolds also has potential for developing optimal seeding patterns to maximize or control tissue growth in the scaffold based on an individual’s physiology. There is also a need for …
Evaluation Of Cellulose Nanocrystal Inks And Their Structural Characteristics For 3d Bioprinting Of Customized Scaffolds, Patrick William Kuczwara
Evaluation Of Cellulose Nanocrystal Inks And Their Structural Characteristics For 3d Bioprinting Of Customized Scaffolds, Patrick William Kuczwara
Graduate Theses and Dissertations
3D bioprinting of biological scaffolds requires control of the physicochemical properties of each unique structures. A promising material for control of properties is hydrogels, which can help create biomimetic scaffolds with controlled spatial arrangement of materials by integrating biological materials directly into layers during the bioprinting process. Nanocellulose offers a unique combination of properties including mechanical, biomimetic, and biocompatibility. These properties offer flexibility over the types, shapes, and applications of their printed hydrogel scaffolds, (i.e., tissue, drug, encapsulation). However, 3D bioprinting of nanocellulose-based hydrogels requires high loading percentages (i.e., >10 wt%) or chemical crosslinkers (i.e., bis(acyl)phosphane oxides (BAPO)). High solid …
Fiber-Based Time-Correlated Single Photon Counting As A Method For Investigating Metabolism During Tetanic Contraction Of Skeletal Muscle, Kathryn Miller Priest
Fiber-Based Time-Correlated Single Photon Counting As A Method For Investigating Metabolism During Tetanic Contraction Of Skeletal Muscle, Kathryn Miller Priest
Graduate Theses and Dissertations
Nicotinamide adenine dinucleotide (NAD) is a cofactor that serves to shuttle electrons during metabolic processes such as glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation (OXPHOS) [1-5]. The reduced form of NAD, NADH, is autofluorescent, making it a measurable characteristic in the investigation of metabolic changes. The fluorescence lifetime of NADH is dependent on the state of protein binding, specifically free or bound NADH, allowing for detection of metabolic shifts between glycolysis (free NADH) and OXPHOS (bound NADH) [6-16]. Fiber-coupled time-correlated single photon counting (TCSPC) equipped with an implantable needle probe can be used to measure NADH lifetime in vivo …
Performance And Fouling During Bioreactor Harvesting, Da Zhang
Performance And Fouling During Bioreactor Harvesting, Da Zhang
Graduate Theses and Dissertations
Tangential flow filtration has many advantages for bioreactor harvesting as the permeate could be introduced directly to the subsequent capture step, the process is easy to scale up, and fouling of the filter is limited by the cross flow. However, membrane fouling has limited its widespread use. This is particularly problematic given the high cell densities encountered today. Here a reverse asymmetric commercial membrane, BioOptimal™ MF-SL (Asahi Kasei), where the more open surface faces the feed stream, and the tighter barrier layer faces the permeate stream, has been investigated for bioreactor harvesting. The open surface contains pores up to 40 …
Fabrication And Non-Covalent Functionalization And Characterization Of Graphene-Based Devices On Novel Substrate Cadmium Trithiophosphate (Iv) — Cdps, Abayomi Omotola Omolewu
Fabrication And Non-Covalent Functionalization And Characterization Of Graphene-Based Devices On Novel Substrate Cadmium Trithiophosphate (Iv) — Cdps, Abayomi Omotola Omolewu
Graduate Theses and Dissertations
With graphene at the center of several application areas such as sensing, circuits, high-frequency devices for communication systems, etc., it is crucial to understand how the intrinsic properties of devices made from graphene and other materials like platinum and palladium nanoparticles affect the performance of such devices for the specific application area. Many graphene-based devices for different application areas have focused mainly on the material composition of the graphene-based devices and how it affects performance parameters for the specific application. However, it would be insightful to understand how the intrinsic electrical properties of the graphene devices for different applications affect …