Biomedical Engineering and Bioengineering Commons^™

Comparative Performance Of Physiological Vital-Sign Forecasting Under Random And Patient-Wise Splitting Using Deep Learning, Lavanya Vasavi Chittem Reddy

Theses and Dissertations

Physiological vital-sign forecasting estimates future measurements based on recent temporal patterns and can support analysis of continuously recorded monitoring data. This study comparatively evaluated deep feedforward, recurrent, bidirectional recurrent, long short-term memory, and bidirectional long short-term memory architectures for one-step-ahead forecasting of peripheral oxygen saturation, heart rate, and pulse rate. Each model received consecutive observations of peripheral oxygen saturation, heart rate, pulse rate, respiratory rate, and age, while separate single-output models predicted the next value of the selected target.

Random and patient-wise data splitting were compared using identical input definitions, preprocessing procedures, model architectures, and training hyperparameters. The strongest architecture …

Enhancing The Performance Of Brain-Computer Interfaces Via A Spatially-Aware Framework, Ahmed Ayman Eloraby

Theses and Dissertations

Electroencephalography (EEG)-based brain–computer interfaces (BCIs) provide a non-invasive pathway for communication, with the P300 speller being one of the most established paradigms. Despite its practicality, EEG signals present inherent challenges, including low signal-to-noise ratio, high dimensionality, and limited spatial resolution. These characteristics complicate the extraction of discriminative spatial patterns, which are critical for accurate P300 detection and character-level decoding.

This thesis addresses these challenges through the development of AttenPCA-Net, a deep learning framework that integrates spatially-aware processing with dimensionality reduction. Principal Component Analysis (PCA) is employed to reduce redundancy and noise while preserving the most informative components of the EEG …

Injectable, In Situ Forming Peptide Nanofiber Reinforced Composite Hydrogels For Age-Related Macular Degeneration, Shambhavi Bagewadi Ms

Theses and Dissertations

Cell therapy for age-related macular degeneration (AMD) is limited by the absence of a functional substrate such as Bruch’s membrane (BM), which is critical for retinal pigment epithelial (RPE) cell survival and integration. Although preformed substrates have been explored, their implantation can disrupt the fragile retinal architecture, compromise ocular immune privilege and increase infection risk. To address these limitations, an injectable hydrogel capable of forming in situ was developed. The hydrogel consists of thiol-modified hyaluronic acid (HA-SH) and polyvinyl alcohol (PVA). Peptide nanofibers were incorporated to enhance structural reinforcement. Three self-assembling peptides - RADA-16, GAGA and GAGA-YIGSR were designed and …

Mechanical Characterization And Modeling Of Rat Myocardia Under The Influence Of Epirubicin, Abdallah Mahmoud Alkhaiyat

Theses and Dissertations

Cancer remains a predominant health challenge that is responsible for a significant portion of global morbidity and mortality. Epirubicin (EPI), a chemotherapeutic anti-cancer drug, has shown remarkable efficacy in combating various malignancies. However, it is known to have undesirable side effects on the heart, collectively referred to as cardiotoxicity. This research investigates the adverse effects of chemotherapy on cardiac contractility through an in vitro examination of the mechanics of healthy and infarcted animal heart tissues. Electrically stimulated slices of rat ventricular tissue were tested using an isometric force measurement tissue bath. The tissue slices were subjected to uniaxial stretching, allowing …

A Virtual-Reality-Based Dental Simulator For Endodontics With Automated Formative Feedback, Yousef Salah Abo El Ela

Theses and Dissertations

Advancements in virtual reality (VR) and haptic technology are transforming the landscape of medical and dental education, offering new avenues for safe, immersive, and repeatable training experiences. Within dentistry, endodontics presents unique challenges for preclinical education due to anatomical complexity, limited access to extracted teeth, ethical concerns, and the shortcomings of conventional plastic models. Despite endodontics specific plastic teeth being available, they fall short of replicating the hardness of real extracted teeth, are relatively costly compared to generic plastic teeth, and are ultimately a disposable item which makes them inadequate as a sustainable long-term solution. Extracted teeth do a much …

De Novo Protein Binding To Zinc Oxide Through Biomineralization Pathways, Jean-Mark A. Francis

Theses and Dissertations

De novo proteins are structurally distinct from proteins found in nature and thus capable of having their amino acid sequence modified to accomplish tasks such as increasing protein-nanoparticle binding without unfolding or decomposing. Zinc Oxide nanoparticles are functionally distinct from their bulk counterparts and are widely used as semiconductors in a variety of fields such as medicine and agriculture. With demand for these nanoparticles increasing, environmentally sustainable methods of Zinc Oxide nanoparticle synthesis are being investigated as an eco-friendly alternative to currently utilized but environmentally hazardous chemical and physical techniques. This research investigates the binding characteristics between Zinc Oxide nanoparticles …

Regulation Of Tgf-Β1–Induced Epithelial–Mesenchymal Transition By Integrin Inhibition, Wesam Elhawabri

Theses and Dissertations

Epithelial–mesenchymal transition (EMT) is a critical process underlying cancer progression, fibrosis, and tissue remodeling, characterized by coordinated changes in cell morphology, nuclear structure, and extracellular matrix (ECM) organization. Transforming growth factor beta 1 (TGF-β1) is a primary inducer of EMT; however, the extent to which integrin-mediated signaling and ECM remodeling regulate these structural changes remains incompletely understood. In this study, we quantitatively investigated EMT-associated remodeling in A549 lung carcinoma cells and MCF10A epithelial cells following TGF-β1 stimulation. Cells were cultured on ECM-coated substrates and treated with TGF-β1 in the presence or absence of integrin-targeting inhibitors (ATN-161, 28-G11, and STX-100). Im …

Empirical Dynamical Modeling Of Cortico-Hippocampal Information Transfer During Memory Encoding, Sam Y. Cole

Theses and Dissertations

Understanding how brain dynamics support successful memory formation remains a central challenge in neuroscience. This dissertation applies tools from empirical dynamical systems theory to investigate the neural mechanisms underlying episodic encoding, with a focus on cortico-hippocampal interactions. Using stereoelectroencephalography (sEEG) data recorded during a delayed free recall of word lists task, this work reveals nonlinear, state-dependent shifts in directed information flow between cortex and hippocampus that distinguish successful from unsuccessful encoding. To further characterize these dynamics, state-space trajectories are reconstructed using time-delay embedding and recurrence-based methods, and features derived from these trajectories are used to classify memory outcomes. By integrating …

Comparative Assessment Of Lower Limb Neuromusculoskeletal Activation And Joint Kinematics In Normal And Impaired Gait, Jack Ryan Schule

Theses and Dissertations

This thesis evaluates whether accessible and affordable technologies can provide clinically significant, quantifiable gait analysis comparable to traditional marker-based motion capture systems. Modern high-end systems offer excellent accuracy but remain financially and logistically inaccessible to many clinicians and researchers. To address this gap, a framework was developed using markerless pose estimation using the model, MediaPipe, inertial measurement units (IMUs) processed through an Extended Kalman Filter (EKF), and surface electromyography (sEMG) to measure joint kinematics and neuromuscular activity during gait. An experiment was performed where data was collected from participants with both normal gait and pathologies including: Osgood–Schlatter disease, Plica Band …

Gut Microbiome Dynamics In Heart Failure And The Therapeutic Potential Of Nmeg-Cgrp, Kamryn Michael Gleason

Theses and Dissertations

Heart failure (HF) is increasingly recognized as a multisystem disease often linked to gut dysbiosis; however, its specific effects on gut microbial composition remain poorly understood. This study examined long-term changes in the gut microbiome in a murine HF model induced by transverse aortic constriction (TAC) and evaluated the effects of NMEG-CGRP. TAC mimics pressure overload-induced cardiac dysfunction, replicating key features of HF. CGRP, a neuropeptide with vasodilatory and cardioprotective effects, shows potential as a therapy for HF but is limited by rapid degradation. A stabilized analog, NMEG-CGRP, was used to assess its impact on cardiac and gastrointestinal health. Mice …

Multiscale Geometric Analysis In Endovascular Therapies: Ex Vivo, In Silico Approaches, Dima Hussein Ali Bani Hani

Theses and Dissertations

In this work, we address a persistent global challenge of vascular disease, specifically peripheral artery disease (PAD), and arteriovenous fistula (AVF) complications in patients with end-stage kidney disease (ESKD). The important role of vascular geometry in disease intervention plans and the development of endovascular therapeutic strategies (e.g., Drug-coated balloon (DCBs)) is critical in addressing these challenges.

This study evaluates the feasibility of co-delivery of paclitaxel (PTX) and valsartan (VAL) using urea-based coatings in DCB, analyzes the coating morphology and microstructural changes to improve therapeutic results. A computational finite element model was developed to complement experimental work, simulate tissue-coating interactions, compute …

Facile Fabrication Of Protein-Polysaccharide Conjugate Multifilament Nerve Guidance Conduit For Peripheral Nerve Regeneration, Preethy Amruthavarshini R

Theses and Dissertations

Long segmental defects in peripheral nerves impair sensory and motor function by disrupting neural impulse transmission. End-to-end autologous grafting with proper fascicular complementation has demonstrated functional recovery per the Medical Research Council Classification (MRCC). However, obtaining donor nerve tissue exceeding 30 mm remains a significant challenge. Artificial hollow conduits serve as an alternative but are limited to defects smaller than 30 mm due to inadequate innervation across the lumen.

Multichannel nerve guidance conduits (mNGCs) have emerged as a promising solution by enhancing fascicular complementation similar to autografts. This study presents a two-step approach for fabricating nerve conduits with precise fascicular …

Development Of A Wearable Biosensor For Cortisol Detection In Human Sweat Utilizing Pani/Peo Polymer Blend And Nitrogen-Doped Graphene Quantum Dots, Cesar Sanchez

Theses and Dissertations

Since the development of the first biosensor in 1962, the evolution of commercially available wearable biosensors has been driven by an increasing consumer interest in real-time health tracking. However, challenges related to sensor sensitivity and cost have hindered progress. Deploying a novel approach, the biosensor integrates a blend of Polyaniline (PANi) and Polyethylene Oxide (PEO) along with Nitrogen-doped Graphene Quantum Dots (N-GQDs) to enhance sensitivity and electroactivity, crucial for cortisol detection. To optimize performance, three distinct synthesis methods for N-GQDs; hydrothermal, microwave, and hot plate synthesis were compared employing a bottom-up approach utilizing citric acid (CA) as the primary reactant …

Computational And Experimental Analysis Of Knee Kinematics And Gait Variability: The Effects Of Load, Speed, And Fatigue On Human Motion, Alfirio Trejo Jr.

Theses and Dissertations

This thesis investigates the biomechanical behavior of the human knee under varying conditions of motion, load, and fatigue, with a focus on clinical angle analysis and gait stability. Four studies were conducted using motion capture data and a modified Grood and Suntay Joint Coordinate System (JCS) to evaluate knee joint kinematics—specifically flexion-extension, abduction-adduction, and internal-external rotation. The first study examined the effects of incremental weight loads during isolated knee extension exercises, revealing dose-dependent increases in abduction and internal rotation that may contribute to joint stress. The second study assessed the impact of walking speed and asymmetric loading on gait, showing …

Interdigitated Gear-Shaped Screen-Printed Electrode Using G-Pani Ink For Sensitive Electrochemical Detection Of Stress Related Hormones Dopamine And Cortisol, Pritu Parna Sarkar

Theses and Dissertations

This research introduces a novel interdigitated gear-shaped graphene-based electrochemical biosensor for the simultaneous detection of dopamine (DA) and cortisol. Modified with graphene–polyaniline (G–PANI) conductive ink, the sensor increased active surface area—94.52% and 57% higher than Metrohm DropSens 110 and circular printed sensors, respectively. Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR) Spectroscopy and X-ray diffraction (XRD) were employed for physicochemical characterization. The sensor detects dopamine and cortisol over a linear range of 0.1–100 µM, with limits of detection of 0.043 µM (R² = 0.98) and 0.0577 µg/mL (R² = 0.976), respectively, in phosphate-buffered saline (PBS) using redox probe. Electrochemical performance …

Characterizing The Relationships Between Cranial Geometry, Brain Morphometry, And Brain Mechanical Properties Using Mr Imaging And Elastography, Jessica Restivo

Theses and Dissertations

Traumatic brain injury (TBI) is a global health concern, with over 69 million cases annually. TBI risk depends on subject-specific neuroanatomical variation, yet research often only considers generic finite element (FE) models, based on the geometry and material composition of a 50th percentile male head. Although highly accurate, subject-specific models require expensive magnetic resonance (MR) imaging, elastography, and time-consuming computational resources. There is a need to create subject-specific TBI models that account for subject-specific variations, without neuroimaging. This study aims to create mathematical models to predict brain morphometry (dimensions, volume) [Objective 1] and mechanical properties (shear stiffness, damping ratio, octahedral …

Image-Based Models For Left Heart Flow Morphodynamics In Hypertrophic Obstructive Cardiomyopathy, Abdelrahman A. Sultan

Theses and Dissertations

Relieving flow obstruction in hypertrophic obstructive cardiomyopathy is highly effective in reducing symptoms and promoting left ventricular remodeling. However, existing characteristics in the disease, such as the systolic anterior motion of the mitral, mitral regurgitation, and papillary muscle motion, and their effect on ventricular flow and vortex formation, have not been thoroughly addressed. This study aims to develop image-based computational fluid dynamics models to evaluate flow morphodynamics in hypertrophic obstructive cardiomyopathy. A computational fluid dynamics model is constructed to investigate the interaction between inflow and outflow in hypertrophic obstructive cardiomyopathy, with a focus on how the motion of the left …

Synthesis And Characterization Of Composite Hydrogels For Tissue Engineering: An Investigation On Mechanical Properties And Biocompatibility, Ismael Lamas Jr.

Theses and Dissertations

This study investigates the fabrication and characterization of Polyvinyl Alcohol (PVA)/agar double-network (DN) hydrogels for biomedical applications, focusing on mechanical strength, biocompatibility, and tissue engineering potential. Techniques like swelling ratio analysis and Fourier-transform infrared spectroscopy (FTIR), alongside antibacterial and cytotoxicity assays, were employed to evaluate the hydrogels' properties. Results show that increasing agar content enhances mechanical and physical properties, with swelling ratios increasing by 300% when agar makes up 6% of the hydrogel, compared to 140% without agar. A positive correlation was observed between agar concentration and compressive and tensile moduli. Load-relaxation rates, essential for viscoelastic properties, also improved with …

Blink-Related Brainwaves As Digital Biomarkers For Cognition Assessment During Complex Tasks, Lexine Carmlixa Sibert-Jolissaint

Theses and Dissertations

Blink-related oscillations (BROs) are neurophysiological responses observed in electroencephalography (EEG) following spontaneous blinking, reflecting the brain processing of new visual information. Prior research has demonstrated BRO responses across various cognitive states, such as resting, cognitive loading, and sensory stimulation, highlighting their potential as a non-invasive biomarker of brain function. We conducted the first investigation of BRO responses during a verbal fluency (VF) task, which is a complex, dynamic task state combining memory, visual perception, executive function, and language processing. Using publicly available EEG data from 22 healthy adults, we measured BRO responses over the left anterior frontal region corresponding to …

Online Parameter Adaptation Of Lqr Controllers Via Rls For Prosthetic Joint Control: Experimental Validation On A Quanser Qube-Servo 2 Platform, Cynthia Lopez-Jordan

Theses and Dissertations

This thesis develops and experimentally validates an online adaptive Linear Quadratic Regulator (LQR) control method for prosthetic joint systems using Recursive Least Squares (RLS)-based real-time parameter estimation on the Quanser QUBE-Servo 2 platform. Traditional LQR controllers assume a fxed system model, which limits adaptability and results in reduced tracking accuracy, poor robustness, and loss of optimal performance when applied to dynamically changing prosthetic joints, infuenced by load variations, user gait changes, and mechanical wear. Limited experimental validation exists for combining RLS with online LQR adaptation in prosthetic-like systems.

To address these limitations, an RLS-driven Adaptive LQR framework was implemented to …

Characterization Of Nanoplastic-Induced Exacerbation Of Asthmatic Pathophysiology In A Murine In Vivo Model, Keith Li

Theses and Dissertations

As global plastic production increases, so does the environmental and biological presence of nanoplastics (NPs), raising growing concerns about their potential impact on human health. These particles have been detected in various organs, including the lungs, yet their role in respiratory inflammation remains largely unexplored. Emerging evidence suggests that NP exposure may influence immune responses in the lungs, particularly in individuals with preexisting conditions such as asthma. However, the specific ways in which these particles interact with allergen driven inflammation are still unclear.

In this study, a murine model of allergic airway inflammation was used to investigate how surface modified …

Intervertebral Lateral Bending Moment Differences In The Lumbar Spine Of Baseball Pitchers Across Throwing Velocities, Justin Humenay

Theses and Dissertations

Introduction Baseball pitching imposes substantial mechanical demands on the lumbar spine, a crucial link in the kinetic chain responsible for transferring energy from the lower body to the upper extremity. While increased pitch velocity is associated with greater trunk forces the specific intervertebral loading patterns during high-velocity pitching remain unclear. This study investigates differences in lateral bending moments across lumbar spinal segments between collegiate pitchers throwing at high versus low velocities.

Methods Twenty right-handed collegiate pitchers were divided into two groups: high-velocity (n = 10, 90.3 ± 1.6 mph) and low-velocity (n = 10, 81.6 ± 1.1 mph). Motion capture …

Development Of A Mediation Model Of Pain Cognition In Adult Patients With Sickle Cell Disease, Daniel M. Sop

Theses and Dissertations

Pain is a hallmark of Sickle Cell Disease (SCD), a genetic hemoglobinopathy characterized by chronic hemolytic anemia, vaso-occlusion, and organ damage. While acute vaso-occlusive crises (VOC) have been extensively studied, the mechanisms underlying chronic pain and its interplay with brain function remain unclear. This dissertation investigates the relationships between cerebral blood flow (CBF), fluid cognition, and pain sensitivity in adults with SCD, utilizing advanced imaging techniques and validated assessment tools.

Arterial spin labeling (ASL) and functional magnetic resonance imaging (fMRI) were employed to measure CBF and brain activity, respectively. Pain sensitivity was assessed using the Pain Sensitivity Questionnaire (PSQ), and …

The Bacteriostatic, Regenerative, And Immunomodulatory Properties Of Extracellular Matrix Particles For Lung Injury, Keera P. Rhoads

Theses and Dissertations

Acute respiratory distress syndrome (ARDS) is a prevalent, life-threatening lung condition, affecting nearly 200,000 Americans annually, with a 40% international mortality rate. There is no cure for ARDS, and current pharmacological treatments have limited effectiveness. Symptoms can be mitigated with mechanical ventilation, though this often leads to ventilator-induced lung injuries (VILI) and puts critically ill patients at risk of infections, including ventilator-associated pneumonia (VAP). A promising therapeutic is the extracellular matrix (ECM), a complex network of structural proteins and bioactive molecules that has been shown to have anti-inflammatory properties and prevent fibrosis. We aim to utilize the regenerative and immunomodulatory …

Rehabilitation In Stroke-Related Flexor Synergy, Shawn J. Dirocco

Theses and Dissertations

Upper extremity abnormal flexor synergy caused by stroke can impair individuals by coupling upper extremity muscles with deltoid activation. Previous studies have shown that reduction in deltoid loading can decrease flexor synergy. Robotic rehabilitation has the ability to allow for reduced deltoid loading while assisting or constraining joints along the arm to increase rehabilitation outcomes. The effects of higher degree of freedom low impedance robotics needs to be tested to learn how various parameters affect flexor synergy in comparison to end point robotics. During the first experiment, we tested 16 chronic stroke patients with flexor synergy problems in 3 tasks. …

Modeling Synergistic Effects Of Integrin And Tgf-Β Signaling In Epithelial Mesenchymal Transition, Prerak Thakkar

Theses and Dissertations

The induction of Epithelial Mesenchymal Transition (EMT) is initiated by a

number of protiens and growth factors, one of which is the profibrotic cytokine Trans-

forming Growth Factor β (TGF-β). Within the extracellular matrix (ECM), TGF-β

is found in a latent, inactive form bound to proteins such as fibronectin (FN). For

TGF-β to contribute to EMT induction, it has to be extracted from the latent com-

plexes into a soluble form. This soluble, activated TGF-β can then bind to receptors

on the cell membrane, which causes the transduction of signals that begin EMT. Re-

search has shown that the release …

Strength Imbalances And Shoulder Pain In Manual Wheelchair Users Stratified By Age Of Spinal Cord Injury/Disorder (Sci/D) Onset, Ashlyn M. Jones

Theses and Dissertations

Introduction The majority of manual wheelchair users (MWUs) with spinal cord injury or disorder (SCI/D) develop shoulder pain. In adults with SCI/D, there is evidence that upper limb strength imbalances are associated with shoulder pain. However, strength imbalances may indicate protective compensatory strategies, and other factors may be important contributors to pain. Currently, personalized prevention and management of pain is limited, little is known about joint strength imbalances and pain in children who use manual wheelchairs, and the effect of the age of SCI/D onset is poorly understood. The purpose of this study was to assess the effect of age …

Feasibility Study On Microwave Glucose Level Monitoring, Ricardo Cepeda

Theses and Dissertations

Diabetes management heavily relies on regular blood glucose monitoring, which traditionally involves invasive techniques. In recent years, significant research has been directed towards developing non-invasive glucose monitoring methods, particularly using electromagnetic waves. This study explores the feasibility of detecting blood glucose levels non-invasively by measuring the resonant frequency shifts of an antenna, influenced by the dielectric properties of blood. Microwave measurement techniques have garnered attention due to their ability to safely penetrate human tissue and detect biochemical markers like glucose. While previous studies have established a link between glucose concentration and dielectric properties, many techniques suffer from poor sensitivity or …

Study Of Temperature Effects Of Glucose And Microwave Dielectric Dispersion Model, Ethan Grant Boone

Theses and Dissertations

In this study, the temperature effect of dielectric properties in various aqueous solutions containing glucose are examined. A design for a new testing chamber is introduced to provide improved conditions for testing to ensure accurate and precise measurements. A N-SMA Adaptor is used when obtaining dielectric characteristics of glucose solutions ranging 100-300mg/dl. Dielectric parameters are obtained adopting a modified Debye dispersion model. MATLAB is used to confirm coaxial dimensions are suitable fabrication designs using fixed dielectric parameters of air and water. Simulations and design models were implemented utilizing ANSYS High Frequency Structure Simulator and 3-D Modeler software to develop the …

Advancing Biofabrication Methodologies To Develop Biomimetic Tissue Scaffolds For Musculoskeletal Applications, Nashaita Yezdi Patrawalla

Theses and Dissertations

Musculoskeletal injuries impose a significant global burden, affecting millions and generating an economic toll exceeding $14 billion. Tissue engineering has gained traction as a promising strategy offering potential for functional restoration of damaged musculoskeletal tissues. This research aims to advance biofabrication techniques for generation of biomimetic tissue scaffolds, by optimizing scaffold design and modulating fabrication process parameters to generate scaffolds for musculoskeletal regeneration with application-specific tailored properties. In this realm, collagen-based biomaterials have been extensively investigated for tendon and ligamentous applications, and in combination with bioceramics incorporated into the collagen framework to produce composite scaffolds that better recapitulate the native …