Biomedical Engineering and Bioengineering Commons^™

Effect Of Thermal Treatments On Mechanical Properties And Bioactivity Of Third Generation Of Bioactive Glass. A Step For Commercialization Success Of Third Generation Bioactive Glasses, Rajan Pandya

Theses and Dissertations

Unavailability of donors for transplanting bone to help people with orthopedic imparities gave rise to the metal implants that could be made in factories. This seemed like a good solution as it was available to all the people who needed it. It came with limitations such as restricted movement, infection, wear and tear of the surrounding tissue, and rejection. Biologically active implants were introduced by Dr. Larry Hench in 1960s [12]. The field of regenerative medicine has been changing rapidly since then. Biologically active implants facilitate bone regeneration and do not harm the surrounding tissue in any way. The bioactive …

A Portable Impedance Biosensing System Based On A Laptop With Labview For Rapid Detection Of Avian Influenza Virus, Yixiang Wang

Graduate Theses and Dissertations

Avian Influenza Virus (AIV) H5N1 is a highly pathogenic virus found not only in birds but also in human. Rapid and sensitive detection method is needed to help prevent the spread of AIV H5N1. In this study, a portable impedance biosensing system based on a laptop with LabVIEW software was developed for detection of AIV H5N1. First, a virtual instrument was programmed with LabVIEW software to form a platform for impedance measurement, data processing and control. The audio card of a laptop was used as a function generator while a data acquisition card was used with the signal channels for …

Intention Tremor And Deficits Of Sensory Feedback Control In Multiple Sclerosis: A Pilot Study, Megan Heenan, Robert A. Scheidt, Douglas Woo, Scott A. Beardsley

Biomedical Engineering Faculty Research and Publications

Background

Intention tremor and dysmetria are leading causes of upper extremity disability in Multiple Sclerosis (MS). The development of effective therapies to reduce tremor and dysmetria is hampered by insufficient understanding of how the distributed, multi-focal lesions associated with MS impact sensorimotor control in the brain. Here we describe a systems-level approach to characterizing sensorimotor control and use this approach to examine how sensory and motor processes are differentially impacted by MS.

Methods

Eight subjects with MS and eight age- and gender-matched healthy control subjects performed visually-guided flexion/extension tasks about the elbow to characterize a sensory feedback control model that …

Reversible Blockade Of Complex I Or Inhibition Of Pkcβ Reduces Activation And Mitochondria Translocation Of P66Shc To Preserve Cardiac Function After Ischemia, Meiying Yang, David F. Stowe, Kenechukwu B. Udoh, James S. Heisner, Amadou K.S. Camara

Biomedical Engineering Faculty Research and Publications

Aim

Excess mitochondrial reactive oxygen species (mROS) play a vital role in cardiac ischemia reperfusion (IR) injury. P66^Shc, a splice variant of the ShcA adaptor protein family, enhances mROS production by oxidizing reduced cytochrome c to yield H₂O₂. Ablation of p66^Shc protects against IR injury, but it is unknown if and when p66^Shc is activated during cardiac ischemia and/or reperfusion and if attenuating complex I electron transfer or deactivating PKCβ alters p66^Shc activation during IR is associated with cardioprotection.

Methods

Isolated guinea pig hearts were perfused and subjected to increasing periods …

Smooth Muscle Cell Organization In The Stem Region Of The Gracilis Collateral Circulation In Balb/C Mice, Laura Lenae Burckhardt

Biomedical Engineering

Of the approximately 8 million Americans who suffer from ischemic peripheral arterial occlusive disease (PAOD), many present with intermittent claudication, or pain associated with exercise. Impaired vasodilation of resistance vessels is a potential explanation for this symptom. Occluded arteries can lead to increased flow through collateral vessels, which function as natural bypasses around the obstruction. This increase in blood flow and resulting shear stress can cause outward remodeling, or arteriogenesis, which improves the efficacy of collaterals. However, following femoral artery ligation in a mouse model of chronic ischemia, vasodilation in the stem region of collateral vessels is impaired at day …

Low Molecular Weight Glucosamine/L-Lactide Copolymers As Potential Carriers For The Development Of A Sustained Rifampicin Release System: Mycobacterium Smegmatis As A Tuberculosis Model, Jorge Ragusa

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Tuberculosis, a highly contagious disease, ranks as the second leading cause of death from an infectious disease, and remains a major global health problem. In 2013, 9 million new cases were diagnosed and 1.5 million people died worldwide from tuberculosis. This dissertation aims at developing a new, ultrafine particle-based efficient antibiotic delivery system for the treatment of tuberculosis. The carrier material to make the rifampicin (RIF)-loaded particles is a low molecular weight star-shaped polymer produced from glucosamine (molecular core building unit) and L-lactide (GluN-LLA). Stable particles with a very high 50% drug loading capacity were made via electrohydrodynamic atomization. Prolonged …

Designing A Biomimetic Testing Platform For Actuators In A Series-Elastic Co-Contraction System, Ryan Tyler Schroeder

UNLV Theses, Dissertations, Professional Papers, and Capstones

Actuators determine the performance of robotic systems at the most intimate of levels. As a result, much work has been done to assess the performance of different actuator systems. However, biomimetics has not previously been utilized as a pretext for tuning a series elastic actuator system with the purpose of designing an empirical testing platform. Thus, an artificial muscle tendon system has been developed in order to assess the performance of two distinct actuator types: (1) direct current electromagnetic motors and (2) ultrasonic rotary piezoelectric motors. Because the design of the system takes advantage of biomimetic operating principles such as …

Fabrication And Characterization Of Antioxidant Loaded Liposomes., Brian Gettler

Electronic Theses and Dissertations

Radiation exposure is both a major obstacle in space exploration and an occupational hazard for various careers causing DNA damage and ROS. In order to reduce the effects of radiation, the primary and most explored approach has been focused around mitigation in terms of exposure duration, exposure intensity, and shielding usage. In some situations none of these reduction strategies were viable and so the use of antioxidants is being explored as an alternate strategy for therapeutic purposes. One such antioxidant is curcumin, otherwise known as diferuloylmethane, is a component of turmeric with both antioxidant properties and anti-inflammatory properties. Another antioxidant …

Development Of A Heart Rate Variability Measurement System Using Embedded Electronics, Naresh Kumar Velmurugan

Graduate Theses and Dissertations

Recent advances in embedded electronics have a remarkable influence on the health care system. One of the most important applications is to monitor the health care of the patients at anytime and anyplace. In the last two decades, many researchers have focused mainly on heart rate variability (HRV) measurements. Patient's heart rate variability should be continuously monitored to help them in case of emergency. Under these circumstances, patients are required to have a HRV measuring kit for a constant observation.

The proposed project focuses on the development of a heart rate variability measurement system with the use of embedded electronics. …

Development Of A Transport System For Advancing Tissue Engineering And Cell Identification, Suzanne Mae Tabbaa

All Dissertations

This work centers on the development of a novel passive transport system for two tissue engineering applications – cell distribution and cell separation. This approach relies on a wicking fiber-based system, derived from the textile field that functions by directing and maintaining transport of cells as well as fluids and biomolecules. This system has the ability to enhance cell movement for both the purpose of cell seeding distribution as well as to isolate specific cell types from heterogeneous cell populations.

The success of spinal fusions and large bone defects is often limited by the decreased surrounding vasculature and the ability …

Quantification Of Protein Adhesion Strength To Surface Attached Poly (N- Isopropylacrylamide) Networks By Hydrodynamic Detachment Shear Stresses, Gulnur Sanden

USF Tampa Graduate Theses and Dissertations

Stimuli responsive coatings offer a versatile method by which to manipulate interfacial interactions of proteins in a desired way. However, there exists little guidance as to how the structure of a responsive polymer coating influences adsorption of proteins. In this dissertation, the adsorption behavior of immuglobulin G (IgG) on poly (N-isopropylacryamide) (PNIPAAm) hydrogel coatings was investigated as a function of film thickness. PNIPAAm exhibits a hydrophilic to hydrophobic transition above a critical temperature of ~32°C in aqueous solutions. In this research, through the use of quartz crystal microbalance with dissipation (QCM-D) it was observed that the adsorption was thickness dependent …

Multi-Modal, Surface-Focused Anticoagulation Using Poly-2-Methoxyethylacrylate Polymer Grafts And Surface Nitric Oxide Release, Surbhi Gupta, Kagya Amoako, Ahmed Suhaib, Keith E. Cook

Mechanical and Industrial Engineering Faculty Publications

This study examines platelet adhesion on surfaces that combine coatings to limit protein adsorption along with “anti-platelet” nitric oxide (NO) release. Uncoated and poly-2-methoxyethylacrylate (PMEA) coated, gas permeable polypropylene (PP) membranes were placed in a bioreactor to separate plasma and gas flows. Nitrogen with 100/500/1000 ppm of NO was supplied to the gas side as a proof of concept. On the plasma side, platelet rich plasma (PRP, 1 × 108 cell/mL) was recirculated at low (60)/high (300) flows (mL/min). After 8 hours, adsorbed platelets on PP was quantified via a lactate dehydrogenase assay. Compared to plain PP, the PMEA coating …

Increasing Label-Free Stem Cell Sorting Capacity To Reach Transplantation-Scale Throughput, Melinda Simon, Ying Li, Janahan Arulmoli, Lisa Mcdonnell, Adnan Akil, Jamison Nourse, Abraham Lee, Lisa Flanagan

Faculty Publications

Dielectrophoresis (DEP) has proven an invaluable tool for the enrichment of populations of stem and progenitor cells owing to its ability to sort cells in a label-free manner and its biological safety. However, DEP separation devices have suffered from a low throughput preventing researchers from undertaking studies requiring large numbers of cells, such as needed for cell transplantation. We developed a microfluidic device designed for the enrichment of stem and progenitor cell populations that sorts cells at a rate of 150,000 cells/h, corresponding to an improvement in the throughput achieved with our previous device designs by over an order of …

Conducting Scaffolds For Liver Tissue Engineering, Armin Tahmasbi Rad, Naushad Ali, Hari Shankar R. Kotturi, Mostafa Yazdimamaghani, Jim Smay, Daryoosh Vashaee, Lobat Tayebi

Chemical and Biochemical Engineering Faculty Research & Creative Works

It is known that there is a correlation between a cell membrane potential and the proliferation of the cell. The high proliferation capacity of liver cells can also be attributed to its specific cell membrane potential as liver cell is recognized as one of the most depolarized of all differentiated cells. We hypothesized that this phenomenon can be emphasized by growing liver cells in conducting scaffolds that can increase the electrical communication among the cells. In this article, using tissue engineering techniques, we grew hepatocyte cells in scaffolds with various compositions. It was found that the scaffolds containing conducting polymer …

Effect Of Plasticizer On Oxygen Permeability Of Cast Polylactic Acid (Pla) Films Determined Using Dynamic Accumulation Method, Kurniawan Yuniarto, Bruce A. Welt, Aris Purwanto, Hadi Karia Purwadaria, Ayman Abdellatief, Titi C. Sunarti, Setyo Purwanto

Journal of Applied Packaging Research

Polylactic acid (PLA) is becoming an increasingly important biopolymer for packaging applications. PLA brittleness limits its applicability. This study evaluated PLA properties with increasing amounts of added polyethylene glycol (PEG) plasticizer. Oxygen transmission rate (OTR) of cast films was determined using the newly available Dynamic Accumulation (DA) method. Arrhenius temperature sensitivity of OTR and polymer Permeability was also determined. Permeability of neat PLA is 4.848 ml mm (STP)/m² s kPa; hence, 4.84 ml mm (STP)/m² s kPa, 4.07 ml mm (STP)/m² s kPa and 5.42 ml mm (STP)/m² s kPa by adding PEG 1 %, 5% …

Reference Point Indentation Is Not Indicative Of Whole Mouse Bone Measures Of Stress Intensity Fracture Toughness, Alessandra Carriero, Jan L. Bruse, Karla J. Oldknow, José Luis Millán, Colin Farquharson, Sandra J. Shefelbine

Biomedical Engineering and Sciences Faculty Publications

Bone fragility is a concern for aged and diseased bone. Measuring bone toughness and understanding fracture properties of the bone are critical for predicting fracture risk associated with age and disease and for preclinical testing of therapies. A reference point indentation technique (BioDent) has recently been developed to determine bone's resistance to fracture in a minimally invasive way by measuring the indentation distance increase (IDI) between the first and last indentations over cyclic indentations in the same position. In this study, we investigate the relationship between fracture toughness KC and reference point indentation parameters (i.e. IDI, total indentation distance (TID) …

A Novel 2.5d Culture Platform To Investigate The Role Of Stiffness Gradients On Adhesion-Independent Cell Migration, Mark-Phillip Pebworth, Sabrina A. Cismas, Prashanth Asuri

Bioengineering

Current studies investigating the role of biophysical cues on cell migration focus on the use of culture platforms with static material parameters. However, migrating cells in vivo often encounter spatial variations in extracellular matrix stiffness. To better understand the effects of stiffness gradients on cell migration, we developed a 2.5D cell culture platform where cells are sandwiched between stiff tissue culture plastic and soft alginate hydrogel. Under these conditions, we observed migration of cells from the underlying stiff substrate into the alginate matrix. Observation of migration into alginate in the presence of integrin inhibition as well as qualitative microscopic analyses …

Wear Resistant And Biocompatible Coatings For Medical Devices And Method Of Fabrication, Mary Gilliam, Susan Farhat, Ali Zand

Chemical Engineering Patents

Abstract: A method of forming a biocompatible or biologically inert article for use in an application in which the article will make contact with at least one tissue, organ, or fluid within a human or animal body is provided. The method generally comprises providing an article having an external sur face; selecting chemical precursors; using a means to direct one or more chemical precursors towards or to apply such chemical precursors to the ex ternal surface; activating the chemical precursors by exposing said precursors to atmospheric pressure plasma; and grafting and/or cross-linking the chem ical precursors to form a solid …

Hepatic Carboxylesterase 1 Is Induced By Glucose And Regulates Postprandial Glucose Levels, Jiesi Xu, Liya Yin, Yang Xu, Yuanyuan Li, Munaf Zalzala, Gang Cheng, Yanqiao Zhang

Chemical, Biomolecular, and Corrosion Engineering Faculty Research

Metabolic syndrome, characterized by obesity, hyperglycemia, dyslipidemia and hypertension, increases the risks for cardiovascular disease, diabetes and stroke. Carboxylesterase 1 (CES1) is an enzyme that hydrolyzes triglycerides and cholesterol esters, and is important for lipid metabolism. Our previous data show that over-expression of mouse hepatic CES1 lowers plasma glucose levels and improves insulin sensitivity in diabetic ob/ob mice. In the present study, we determined the physiological role of hepatic CES1 in glucose homeostasis. Hepatic CES1 expression was reduced by fasting but increased in diabetic mice. Treatment of mice with glucose induced hepatic CES1 expression. Consistent with the in vivo study, …

Lower-Extremity Joint Kinematics And Muscle Activations During Semi-Reclined Cycling At Different Workloads In Healthy Individuals, Kamyar Momeni, Pouran D. Faghri, Martinus Evans

Open Access Author Fund Awardees' Articles

Background

A better understanding of lower-extremity muscles’ activation patterns and joint kinematics during different workloads could help rehabilitation professionals with prescribing more effective exercise regimen for elderly and those with compromised muscles. We examined the relative contribution, as well as activation and co-activation patterns, of lower-extremity muscles during semi-reclined cycling at different workloads during a constant cadence.

Methods

Fifteen healthy novice cyclists participated at three 90-second cycling trials with randomly assigned workloads of 0, 50, and 100 W, at a constant cadence of 60 rpm. During all trials, electromyograms were recorded from four lower-extremity muscles: rectus femoris (RF), biceps femoris …