Biomedical Engineering and Bioengineering Commons^™

Osteon Mimetic Scaffolding, Janay Clytus

Senior Theses

The purpose of this research is to provide an alternative to naturally derived bone grafts. There is a gap in the supply of donors and the demand of bone tissue. Artificial scaffold creation can work as an implant and decrease the shortage of bone grafts and increase the range of injuries that can be repaired. Current research focuses on optimizing mechanical properties such as porosity, improving vascularization using cells, and generating osteoconductivity. For osteodifferentiation, mesenchymal stem cells (MSCs) can differentiate into mesodermal lineages such as chondrocytes, osteoblasts, adipocytes, and tenocytes by supplementing cultures with lineage-specific soluble factors (Marchetti). Co-culturing ECFCs …

Increasing Drug Delivery Efficacy Of Drug-Coated Balloons, Alexander Galan, Eric Bidinger, Franco Godoy, Shrusti Patel

Senior Theses

Peripheral artery disease (PAD), the narrowing of peripheral arteries located in areas such as the arms and legs, is a common disease that affects 12% to 20% of people over 65. Numerous therapies have been developed to treat PAD, the most recent technology being drug-coated balloons. Drug-coated balloons release drugs, such as Paclitaxel (PTX), into the arterial wall during balloon angioplasty to locally treat PAD. Current levels of drug transfer from the balloon to the vascular endothelium are at a low 10%-18%. Our Biomedical Engineering Senior Design project aims to increase drug delivery of drug-coated balloons by manipulating the clinically-controllable …

A Strategic Audit Of Medtronic Plc, Jozzy Carter

Honors Program: Senior Projects (Public)

Humans will always have a need for healthcare, and as the world continues to innovate in the tech space, consumers will demand advances in medicine and medical devices. Medtronic is at the forefront of this innovation but faces intense competition in the medical technologies industry. This poses the question: what can Medtronic do to continue its growth and stay competitive in this space? This paper offers an analysis of Medtronic as a medical device company by offering insight to its competitive advantage, financials, business mix, and value chain. It also analyzes the forces that affect Medtronic using SWOT, PEST, and …

Tracing Actin Filament Bundles In Three-Dimensional Electron Tomography Density Maps Of Hair Cell Stereocilia, Salim Sazzed, Junha Song, Julio Kovacs, Willi Wriggers, Manfred Auer, Jing He

Computer Science Faculty Publications

Cryo-electron tomography (cryo-ET) is a powerful method of visualizing the three-dimensional organization of supramolecular complexes, such as the cytoskeleton, in their native cell and tissue contexts. Due to its minimal electron dose and reconstruction artifacts arising from the missing wedge during data collection, cryo-ET typically results in noisy density maps that display anisotropic XY versus Z resolution. Molecular crowding further exacerbates the challenge of automatically detecting supramolecular complexes, such as the actin bundle in hair cell stereocilia. Stereocilia are pivotal to the mechanoelectrical transduction process in inner ear sensory epithelial hair cells. Given the complexity and dense arrangement of actin …

3d Bioprinting Systems For The Study Of Mammary Development And Tumorigenesis, John Reid

Electrical & Computer Engineering Theses & Dissertations

Understanding the microenvironmental factors that control cell function, differentiation, and stem cell renewal represent the forefront of developmental and cancer biology. To accurately recreate and model these dynamic interactions in vitro requires both precision-controlled deposition of multiple cell types and well-defined three-dimensional (3D) extracellular matrix (ECM). To achieve this goal, we hypothesized that accessible bioprinting technology would eliminate the experimental inconsistency and random cell-organoid formation associated with manual cell-matrix embedding techniques commonly used for 3D, in vitro cell cultures. The first objective of this study was to adapt a commercially-available, 3D printer into a 3D bioprinter. Goal-based computer simulations were …

Study Of Abc Membrane Transporters In Single Live Cells, Preeyaporn Songkiatisak

Chemistry & Biochemistry Theses & Dissertations

The multidrug ATP-binding cassette (ABC) membrane transporters (efflux pumps) are found in both prokaryotes and eukaryotes and they can extrude diverse structurally unrelated substrates, such as antibiotics and chemotherapeutic agents out of the cells. The efflux pumps are responsible for multidrug resistance (MDR) and the failure of numerous treatments in infections and cancers. All ABC membrane transporters share a common modular topology containing two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). The underlying molecular mechanisms regarding how the similar structural ABC membrane transporters could selectively extrude a wide variety of substrates and cause MDR, are not yet fully …

Non-Invasive Picosecond Pulse System For Electrostimulation, Ross Aaron Petrella

Electrical & Computer Engineering Theses & Dissertations

Picosecond pulsed electric fields have been shown to have stimulatory effects, such as calcium influx, activation of action potential, and membrane depolarization, on biological cells. Because the pulse duration is so short, it has been hypothesized that the pulses permeate a cell and can directly affect intracellular cell structures by bypassing the shielding of the membrane. This provides an opportunity for studying new biophysics. Furthermore, radiating picosecond pulses can be efficiently done by a compact antenna because the antenna size is comparable to the pulse width. However, all of the previous bioelectric studies regarding picosecond pulses have been conducted in …

Understanding The Mechanical Behavior Of Costal Cartilage At Their Curved Exterior Surface Via A Tactile Sensor With A Built-In Probe For Distributed-Deflection Detection, Jiayue Shen

Mechanical & Aerospace Engineering Theses & Dissertations

This dissertation is aimed to determine the mechanical properties at the exterior surface of costal cartilages (CC) and examine how they vary with the cartilage length and the anatomical sites of CC in the ribcage via conformal indentation testing which is built upon a tactile sensor for distributed-deflection detection. The sensor entails a rectangular Polydimethylsiloxane (PDMS) microstructure sensing-plate integrated with a 5 ×1 transducer array with 0.75mm spatial resolution underneath and a built-in probe of 0.5mm×5mm×3mm above. By pressing the sensor against the exterior surface of a CC tissue with a pre-defined indentation pattern, the sensor conforms to the curved …

Optimization Of Microfluidic Chip Fabrication Via Femtosecond Laser Ablation, Kenneth Aycock

Senior Theses

Microfluidic devices have become staple tools in biomedical research and have a promising future as low cost, point-of-care (POC) diagnostic devices. Despite the advancements in microfluidic device technology, the manipulation and fabrication of these systems can be tedious and expensive. Repeatable techniques in which computer-aided designs are translated into microfluidic systems in a matter of minutes are highly desirable both for researchers and manufacturers. Laser ablation of tape substrates has shown promise in producing cost-effective, rapidly manipulable devices, but the work done thus far has utilized continuous wave lasers that perform suboptimally due to the relatively short wavelengths used and …

Integration Of A Sensory Driven Model For Hand Grasp Function In 3d Printed Prostheses, Austin T. Hetherington

Senior Theses

My Honors Thesis was completed through the Biomedical Engineering senior design project I worked on. Our project took a mechanically operated 3D printed prosthetic hand and automated the process by which it makes a grasp. The purpose of this project was to provide an affordable, automatic prosthetic hand to those either capable and incapable of wrist flexion. The current 3D printed prosthetic models require users to bend their wrist to initiate a simple grasp. However, our design took an alternative approach by using an EMG sensor placed on the ventral side of the forearm to record muscle activity, which, upon …

Comparison Of Thermal Glove Wrist-Hand Orthoses In Their Effectiveness On Rheumatoid Arthritis, Brittany E. Mott

USF Tampa Graduate Theses and Dissertations

Rheumatoid arthritis (RA) is an autoimmune disease affecting nearly 1% of the world’s population with symptoms such as inflammation, pain, and reduced strength [1]. Physicians and scientists work to develop pharmaceuticals and medical devices aimed at decreasing the symptoms associated with RA to better the lives of those affected. One of the most recent developments is the addition of thermal therapy gloves to the array of upper limb orthoses available to patients with RA. It was hypothesized that this study will show that orthoses in the form of thermal therapy gloves are beneficial to the patients by reducing symptoms such …

A Model-Based Approach For Estimation Of Changes In Lumbar Segmental Kinematics Associated With Alterations In Trunk Muscle Forces, Iman Shojaei, Navid Arjmand, Judith R. Meakin, Babak Bazrgari

Biomedical Engineering Faculty Publications

The kinematics information from imaging, if combined with optimization-based biomechanical models, may provide a unique platform for personalized assessment of trunk muscle forces (TMFs). Such a method, however, is feasible only if differences in lumbar spine kinematics due to differences in TMFs can be captured by the current imaging techniques. A finite element model of the spine within an optimization procedure was used to estimate segmental kinematics of lumbar spine associated with five different sets of TMFs. Each set of TMFs was associated with a hypothetical trunk neuromuscular strategy that optimized one aspect of lower back biomechanics. For each set …

Embedded System Design Of Low-Power Wearable Bioelectronic Devices, Matthew S. Hopper

USF Tampa Graduate Theses and Dissertations

The miniaturization of electronics in modern times has enabled the possibility of creating a “continuity of care” using small wearable bioelectronic devices. Using wearable devices, such as the Fitbit or Garmin fitness trackers, allows for the exchange of data between devices which can be used to improve the accuracy of data analysis and thus patient health.

In this thesis work, three wearable bioelectronic devices are proposed: an EOG-based eye-gaze tracking assistive technology device for the physically disabled to control a computer cursor, a battery-operated miniaturized polysomnograph that can store and transmit data wirelessly to sleep technicians and a trauma-detecting personal …

Walking Speed Differentially Alters Spinal Loads In Persons With Traumatic Lower Limb Amputation, Brad D. Hendershot, Iman Shojaei, Julian C. Acasio, Christopher L. Dearth, Babak Bazrgari

Biomedical Engineering Faculty Publications

Persons with lower limb amputation (LLA) perceive altered motions of the trunk/pelvis during activities of daily living as contributing factors for low back pain. When walking (at a singular speed), larger trunk motions among persons with vs. without LLA are associated with larger spinal loads; however, modulating walking speed is necessary in daily life and thus understanding the influences of walking speed on spinal loads in persons with LLA is of particular interest here. Three-dimensional trunk-pelvic kinematics, collected during level-ground walking at self-selected (SSW) and two controlled speeds (~1.0 and ~1.4 m/s), were obtained for seventy-eight participants: 26 with transfemoral …

3d Tissue Engineering, An Emerging Technique For Pharmaceutical Research, Gregory Jensen, Christian Morrill, Yu Huang

Biological Engineering Faculty Publications

Tissue engineering and the tissue engineering model have shown promise in improving methods of drug delivery, drug action, and drug discovery in pharmaceutical research for the attenuation of the central nervous system inflammatory response. Such inflammation contributes to the lack of regenerative ability of neural cells, as well as the temporary and permanent loss of function associated with neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and traumatic brain injury. This review is focused specifically on the recent advances in the tissue engineering model made by altering scaffold biophysical and biochemical properties for use in the treatment of neurodegenerative diseases. …

Mechanical Demands On The Lower Back In Patients With Non-Chronic Low Back Pain During A Symmetric Lowering And Lifting Task, Iman Shojaei, Elizabeth G. Salt, Quenten L. Hooker, Babak Bazrgari

Biomedical Engineering Faculty Publications

There is limited information in the literature related to the lower back loading in patients with LBP, particularly those with non-chronic LBP. Toward addressing such a research gap, a case-control study was conducted to explore the differences in lower back mechanical loads between a group of females (n=19) with non-chronic, non-specific LBP and a group of asymptomatic females (n=19). The differences in lower back mechanical loads were determined when participants completed one symmetric lowering and lifting of a 4.5 kg load at their preferred cadence. The axial, shearing, and moment components of task demand at the time of peak moment …

Radiotherapy-Induced Cherenkov Luminescence Imaging In A Human Body Phantom, Syed Rakin Ahmed, Mengyu Jia, Petr Bruza, Sergei A. Vinogradov, Shudong Jiang, David J. Gladstone, Lesley A. Jarvis, Brian W. Pogue

Dartmouth Scholarship

Radiation therapy produces Cherenkov optical emission in tissue, and this light can be utilized to activate molecular probes. The feasibility of sensing luminescence from a tissue molecular oxygen sensor from within a human body phantom was examined using the geometry of the axillary lymph node region. Detection of regions down to 30-mm deep was feasible with submillimeter spatial resolution with the total quantity of the phosphorescent sensor PtG4 near 1 nanomole. Radiation sheet scanning in an epi-illumination geometry provided optimal coverage, and maximum intensity projection images provided illustration of the concept. This work provides the preliminary information needed to attempt …

Combining Thermo-Plasmonics With Microfluidics For Biological Applications, Sharad Ambardar

USF Tampa Graduate Theses and Dissertations

In this project we, for the first time, integrated microfluidics with thermo-plasmonics. While microfluidics is a popular platform allowing experiments with small volumes of fluid, thermo-plasmonics can be used for powerful particle manipulation including capturing, mixing, filtering and projection. Combined, these two techniques give us an opportunity to work with numerous complex fluids containing particles, cells, and micro-beads. Here we designed, developed and tested several devices demonstrating various aspects of this exciting hybrid technology. This required use of soft lithography, metal deposition, 3D printing, oxygen plasma treatment and several other surface modification techniques. Additional challenges were in the fabrication of …

Engaging Biomedical Engineering In Health Disparities Challenges, Maribel Vazquez

Publications and Research

Health Disparities (HD) are community-based, biomedical challenges in need of innovative contributions from Science, Technology, Engineering and Math (STEM) fields. Surprisingly, STEM professionals demonstrate a persistent lack of HD awareness and/or engagement in both research and educational activities. This project introduced Health Disparities (HD) as technical challenges to incoming undergraduates in order to elevate engineering awareness of HD. The objective was to advance STEM-based, HD literacy and outreach to young cohorts of engineers. Engineering students were introduced to HD challenges in technical and societal contexts as part of Engineering 101 courses. Findings demonstrate that student comprehension of HD challenges increased …

Enucleated Cells Reveal Differential Roles Of The Nucleus In Cell Migration, Polarity, And Mechanotransduction, Gunes Uzer

Mechanical and Biomedical Engineering Faculty Publications and Presentations

The nucleus has long been postulated to play a critical physical role during cell polarization and migration, but that role has not been defined or rigorously tested. Here, we enucleated cells to test the physical necessity of the nucleus during cell polarization and directed migration. Using enucleated mammalian cells (cytoplasts), we found that polarity establishment and cell migration in one dimension (1D) and two dimensions (2D) occur without the nucleus. Cytoplasts directionally migrate toward soluble (chemotaxis) and surface-bound (haptotaxis) extracellular cues and migrate collectively in scratch-wound assays. Consistent with previous studies, migration in 3D environments was dependent on the nucleus. …