Biomedical Engineering and Bioengineering Commons^™

Conference Program, Dolores Baksh, Rod Rietze, Ivan Wall

Advancing Manufacture of Cell and Gene Therapies VI [2019]

No abstract provided.

Workshop Agenda, Fernanda Masri

Advancing Manufacture of Cell and Gene Therapies VI [2019]

No abstract provided.

Development Of A Closed Car-T Manufacturing Process, Loo-Yong Steven Kee, Calley Hirsch, Devina Ramsaroop, Elizabeth Csaszar, Danylo Sirskyj, Aaron Dulgar-Tulloch

Advancing Manufacture of Cell and Gene Therapies VI [2019]

The field of immunotherapy has emerged as a promising new type of treatment for cancer with the approval of the first two CAR-T therapies. The clinical success of T-cell based immunotherapies necessitates a robust manufacturing process for these products to be consistently produced at commercial scale. Our CAR-T workflow combines unit operation specific solutions for thaw of an apheresis unit, wash, CD3 selection, T-cell activation, lentiviral transduction, incubator- and reactor-based expansion culture, harvest, formulation, cryopreservation and thaw of CAR-T product. We have evaluated the impact of both serum-containing and xeno-free culture media, commercially available T-cell selection and activation reagents, closed …

Scale-Up Study For Ex-Vivo Expansion Of Allogeneic Natural Killer Cells In Stirred-Tank Bioreactor, Juyoung Kim, Hyung Jin Nam, Sang Hyun Lee, Jong Beom Ku, Seung Ryel Han, Hye Jin Shin, Yu Kyeong Hwang, Sang Hoon Paik

Advancing Manufacture of Cell and Gene Therapies VI [2019]

Natural killer (NK) cells are a type of lymphocyte in the blood that are responsible for innate and adaptive immune response, and they mature in the liver and bone marrow. Being a key role in host defense system with direct and indirect killing of virus-infected cells or cancer cells, NK cell has been considered an attractive candidate for cancer therapy. Peripheral blood shows the low frequency of NK cells, so ex vivo expansion method is important to obtain sufficient NK cells for therapeutic use. Currently, we successfully developed bioreactor process for NK cell expansion on lab-scale. Stirred-tank bioreactor could be …

Single Use Disposable Biosettler Removes The Dead Cells And Cell Debris Selectively To Increase The Viability Percentage Of Mammalian Cells (E.G., Car-T) During Expansion, Dhinakar Kompala

Advancing Manufacture of Cell and Gene Therapies VI [2019]

Current challenge in a FDA approved cell therapy product for adult B-cell leukemia is reported to be the percentage of viable cells after manufacturing is sometimes out of specified range. As the head of a large contract development and manufacturing organization observed, this fall in viability percentage during CAR-T cell manufacturing is a challenge to the whole industry.

We present a simple and powerful off-the-shelf solution to this big challenge in all mammalian cell culture expansion bioreactor system. Our single use disposable BioSettler has been demonstrated to be uniquely capable of removing dead cells and cell debris selectively from the …

Disciplinary Learning From An Authentic Engineering Context, Catherine Langman, Judith Zawojewski, Patricia Mcnicholas, Ali Cinar, Eric Brey, Mustafa Bilgic, Hamidreza Mehdizadeh

Journal of Pre-College Engineering Education Research (J-PEER)

This small-scale design study describes disciplinary learning in mathematical modeling and science from an authentic engineeringthemed module. Current research in tissue engineering served as source material for the module, including science content for readings and a mathematical modeling activity in which students work in small teams to design a model in response to a problem from a client. The design of the module was guided by well-established principles of model-eliciting activities (a special class of problem-solving activities deeply studied in mathematics education) and recently published implementation design principles, which emphasize the portability of model-eliciting activities to many classroom settings.

Two …

The Effect Of Electrospun Fiber Diameter On Astrocyte-Mediated Neurite Guidance And Protection, Christopher D.L. Johnson, Jonathan M. Zuidema, Kathryn R. Kearns, Alianna B. Maguire, Gregory P. Desmond, Deanna M. Thompson, Ryan J. Gilbert

Chemical and Biochemical Engineering Faculty Research & Creative Works

The topography of electro spun fiber scaffolds modifies astrocytes toward in vivo-like morphologies and behaviors. However, little is known about how electro spun fiber diameter influences astrocyte behavior. In this work, aligned fibers with two distinct nanoscale fiber diameters (808 and 386 nm) were prepared, and the astrocyte response was measured over time. Astrocytes on the large diameter fibers showed significantly increased elongation as early as 2 h after seeding and remained significantly more elongated for up to 4 days compared to those on small diameter fibers. Astrocytes extending along larger diameter fibers were better equipped to support long neurite …

Coatings On Mammalian Cells: Interfacing Cells With Their Environment, Kara A. Davis, Pei-Jung Wu, Calvin F. Cahall, Cong Li, Anuhya Gottipati, Brad J. Berron

Chemical and Materials Engineering Faculty Publications

The research community is intent on harnessing increasingly complex biological building blocks. At present, cells represent a highly functional component for integration into higher order systems. In this review, we discuss the current application space for cellular coating technologies and emphasize the relationship between the target application and coating design. We also discuss how the cell and the coating interact in common analytical techniques, and where caution must be exercised in the interpretation of results. Finally, we look ahead at emerging application areas that are ideal for innovation in cellular coatings. In all, cellular coatings leverage the machinery unique to …

Ensuring Scientific Publishing Credibility In Translational Biomedical Optics., Brian W. Pogue

Dartmouth Scholarship

Optics has consistently been the largest singular technology sector used in medicine, and major advances in biomedical optics are documented daily in peer-reviewed publications. However, the academic stature of this field can be damaged by weaknesses in scientific publishing, where a “credibility crisis” has emerged as a popularized and increasingly studied dialogue. While there are still relatively few overt cases of fraud or erroneous research, more insidious aspects are seen in papers with results that have either low statistical power, selective reporting of observations, or data or computer codes that cannot be independently verified. Interestingly, the same solutions that improve …

3d Microwave Tomography Using The Soft Prior Regularization Technique: Evaluation In Anatomically-Realistic Mri-Derived Numerical Breast Phantoms, Amir H. Golnabi, Paul M. Meaney, Shireen D. Geimer, Keith D. Paulsen

Department of Mathematics Faculty Scholarship and Creative Works

Objective: Fusion of magnetic resonance imaging (MRI) breast images with microwave tomography is accomplished through a soft prior technique, which incorporates spatial information (from MRI), i.e. accurate boundary location of different regions of interest, into the regularization process of the microwave image reconstruction algorithm. Methods: Numerical experiments were completed on a set of 3D breast geometries derived from MR breast data with different parenchymal densities, as well as a simulated tumor to evaluate performance over a range of breast shapes, sizes and property distributions. Results: When the soft prior regularization technique was applied, both permittivity and conductivity relative root mean …

Injectable, Magnetically Orienting Electrospun Fiber Conduits For Neuron Guidance, Christopher D.L. Johnson, Debmalya Ganguly, Jonathan M. Zuidema, Thomas J. Cardinal, Alexis M. Ziemba, Kathryn R. Kearns, Simon M. Mccarthy, Deanna M. Thompson, Ganpati Ramanath, Diana A. Borca-Tasciuc, Silvio Dutz, Ryan J. Gilbert

Chemical and Biochemical Engineering Faculty Research & Creative Works

Magnetic electro spun fibers are of interest for minimally invasive biomaterial applications that also strive to provide cell guidance. Magnetic electro spun fibers can be injected and then magnetically positioned in situ, and the aligned fiber scaffolds provide consistent topographical guidance to cells. In this study, magnetically responsive aligned poly-l-lactic acid electro spun fiber scaffolds were developed and tested for neural applications. Incorporating oleic acid-coated iron oxide nanoparticles significantly increased neurite outgrowth, reduced the fiber alignment, and increased the surface nano topography of the electro spun fibers. After verifying neuron viability on two-dimensional scaffolds, the system was tested as an …

The Effect Of Impact Location On Brain Strain, Stephen Tiernan, Gary Byrne Mr

Articles

Objective: To determine the effect of impact direction on strains within the brain.

Research design: Laboratory drop tests of hybrid III head-form and finite element simulation of impacts.

Methods and procedures: A head-form instrumented with accelerometers and gyroscopes was dropped from 10 different heights in four orientations: front, rear, left and right hand side. Twelve impacts with constant impact energy were chosen to simulate, to determine the effect of impact location. A finite element head model was used to simulate these impacts, using 6 degrees of freedom. Following this a further set of simulations were performed, where the same acceleration …

Communication Of Deaf People Based On Myoware Muscle Sensor, Mohamad Ataya

Research Opportunities for Engineering Undergraduates (ROEU) Program 2018-19

The current project aims to create a product that enhances communication of deaf people. The developed product has the following features:

• a muscle sensor that transcribes sign language into actual letters

• a voice detector that translates speech into actual words

• the ability to receive and send emergency alerts.

Exploring The Challenges To Sustainable Rural Drinking Water Services In Chile, Jami Nelson-Nuñez, Jeffrey P. Walters, Denisse Charpentier

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

Many countries around the world now face the dual challenges of closing the remaining gaps in access to drinking water in rural areas while further addressing the issues of equity, quality, and sustainability outlined in the new Sustainable Development Goals (SDGs). Our research explores the key factors for sustainability in rural drinking water services in Chile, an important example not only due to its success in rural water access but also because of the new directions the country is taking to achieve the SDGs. Drawing on results from a Delphi study of Chilean rural water experts, we discuss the most …

Progressive Hypoxia-On-A-Chip: An In Vitro Oxygen Gradient Model For Capturing The Effects Of Hypoxia On Primary Hepatocytes In Health And Disease, Young Bok Abraham Kang, Jinsu Eo, Beyza Bulutoglu, Martin L. Yarmush, O. Berk Usta

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

Oxygen is vital to the function of all tissues including the liver and lack of oxygen, that is, hypoxia can result in both acute and chronic injuries to the liver in vivo and ex vivo. Furthermore, a permanent oxygen gradient is naturally present along the liver sinusoid, which plays a role in the metabolic zonation and the pathophysiology of liver diseases. Accordingly, here, we introduce an in vitro microfluidic platform capable of actively creating a series of oxygen concentrations on a single continuous microtissue, ranging from normoxia to severe hypoxia. This range approximately captures both the physiologically relevant oxygen gradient …

Cancer Nanotechnology: Enhancing Tumor Cell Response To Chemotherapy For Hepatocellular Carcinoma Therapy, Sun Yongbing, Ma Wen, Yang Yuanyuan, He Mengxue, Li Aimin, Bai Lei, Yu Bin, Yu Zhiqiang

Faculty & Staff Scholarship

Abstract

Hepatocellular carcinoma (HCC) is one of the deadliest cancers due to its complexities, reoccurrence after surgical resection, metastasis and heterogeneity. In addition to sorafenib and lenvatinib for the treatment of HCC approved by FDA, various strategies including transarterial chemoembolization, radiotherapy, locoregional therapy and chemotherapy have been investigated in clinics. Recently, cancer nanotechnology has got great attention for the treatment of various cancers including HCC. Both passive and active targetings are progressing at a steady rate. Herein, we describe the lessons learned from pathogenesis of HCC and the understanding of targeted and non-targeted nanoparticles used for the delivery of small …

Feasibility Of Integrating Microfluidics Into Point-Of-Care Bio-Diagnostics, Swetha Manoharan

Theses

Potassium in the body needs to be maintained in proper levels for the normal functioning of heart and kidneys. The aim of this work is to develop a simple technique to measure the concentration of Potassium present in a bodily fluid sample, which can be used to assess the health condition of extremely sick patients, especially when the patient's condition needs to be diagnosed and results needs to be obtained rapidly under Point-of-Care settings. Microfluidics is one of the platforms used in bio-diagnostics because of several advantages such as low sample volume requirement and integration into high-throughput screening. In this …

Abso2luteu-Net: Tissue Oxygenation Calculation Using Photoacoustic Imaging And Convolutional Neural Networks, Kevin Hoffer-Hawlik, Geoffrey P. Luke

ENGS 88 Honors Thesis (AB Students)

Photoacoustic (PA) imaging uses incident light to generate ultrasound signals within tissues. Using PA imaging to accurately measure hemoglobin concentration and calculate oxygenation (sO₂) requires prior tissue knowledge and costly computational methods. However, this thesis shows that machine learning algorithms can accurately and quickly estimate sO₂. absO₂luteU-Net, a convolutional neural network, was trained on Monte Carlo simulated multispectral PA data and predicted sO₂ with higher accuracy compared to simple linear unmixing, suggesting machine learning can solve the fluence estimation problem. This project was funded by the Kaminsky Family Fund and the Neukom Institute.

Bioelectrical Circuits: Lecture 7, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.

Assessing The Role Of Polyphenols As A Vascular Protectant Against Drug Induced Vascular Injury, Anson Jacob Oommen

Browse all Theses and Dissertations

Vascular injury is identified during pre-clinical toxicity testing within certain pharmacological classes of drug candidates and induces degenerative and hyperplastic changes in endothelial (ECs) and vascular smooth muscle (VSMCs) cells. This drug-induced vascular injury has been show as a side-effect caused by various classes of drugs, including, antibacterial (e.g., azithromycin), anti-malarial (e.g., quinoline), anti-viral (e.g., anti-hepatitis C virus interferons) and nonsteroidal anti-inflammatory drugs (e.g., ibuprofen), and affects normal cardiovascular function and can further lead to various cardiovascular conditions like arrhythmia, cardiac arrest, high blood pressure, and even heart attack. The search for agents capable of reducing vascular injury side-effects by …