Biomedical Engineering and Bioengineering Commons^™

Bioelectrical Circuits: Lecture 5, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.

Bioelectrical Circuits: Lecture 10, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.

Bioelectrical Circuits: Lecture 6, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.

Mitochondria-Dependent Cellular Toxicity Of Α-Synuclein Modeled In Yeast, Rajalakshmi Santhanakrishnan

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Parkinson’s disease is the second most common neurodegenerative disease. This disease is caused by the degeneration of dopaminergic neurons, leading to debilitating motor symptoms and early mortality. The protein α-synuclein (α-syn), encoded by SNCA, misfolds and forms inclusions in Parkinson’s disease brains. When α-syn is overexpressed in yeast, it causes cellular toxicity and an increased number of aggregates, recapitulating the toxic phenotypes observed in humans and animal models. Yeast models are a powerful tool to perform high-throughput overexpression screening to identify modifiers of α-syn toxicity. α-syn causes mitochondrial dysfunction by inhibiting complex I and inducing mitochondrial fragmentation. Prior screening of …

Biom 3110: Medical Physiology (Syllabus), Bradford D. Pendley

Biomedical Engineering Syllabi Archive

Course Description: Basic principles of human physiology and how these are applied in medicine and biomedical engineering problem and devices; physiological principles using appropriate mathematical and engineering models to construct an understanding of how the body works as an integrated system.

Bioelectrical Circuits: Lecture 4, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.

Bme 20500 Bioelectrical Circuits, Jacek P. Dmochowski, Luis Cardoso

Open Educational Resources

No abstract provided.

Development Of A Novel Ultrasound/Photoacoustic Tomography System For Breast Cancer Imaging Using Full-Ring Illumination Mode, Suhail Salem Alshahrani

Wayne State University Dissertations

Breast cancer is a significant health problem, not only in the United States but globally. In the last year, breast cancer was the second leading cause of cancer deaths in the United States. In addition, the incidence rates of invasive and in situ female breast cancer increased in all age classes between 1975 and 2014. Early detection is a critical component in effectively managing this disease. One of the challenges of early detection of breast cancer lies in the availability and accuracy of diagnostic imaging. Several modalities for early detection are currently available and in use. One of the most …

Initiation Of Action Potential In Murine Ventricular Myocytes By Bursts Of Nanosecond Pulses, Iurii Semenov

Bioelectrics Publications

[First slide] Current situation

I. Among 300,000 persons die from sudden cardiac arrest every year over 40% are victims of fibrillation

II. Defibrillation by an electric shock is the effective therapy against sudden cardiac death

III. Current approach to stop fibrillation may involve disruption of plasma membrane (PM) by an electroporation

IV. Electroporation is damaging, it can: I. Induce post shock arrhythmias II. Complicate patient's recovery III. Lead to death after initially successful resuscitation

Nanosecond Pulsed Electric Signals Can Affect Electrostatic Environment Of Protiens Below The Threshold Of Conformational Effects: The Case Study Of Sod1 With A Molecular Simulation Study, Elena Della Valle, Paolo Marracino, Olga Pakhomova, Micaela Liberti, Francesca Apollonio

Bioelectrics Publications

Electric fields can be a powerful tool to interact with enzymes or proteins, with an intriguing perspective to allow protein manipulation. Recently, researchers have focused the interest on intracellular enzyme modifications triggered by the application of nanosecond pulsed electric fields. These findings were also supported by theoretical predictions from molecular dynamics simulations focussing on significant variations in protein secondary structures. In this work, a theoretical study utilizing molecular dynamics simulations is proposed to explore effects of electric fields of high intensity and very short nanosecond duration applied to the superoxide dismutase (Cu/Zn-SOD or SOD-1), an important enzyme involved in the …

Selective Distant Electrostimulation By Synchronized Bipolar Nanosecond Pulses, Elena C. Gianulis, Maura Casciola, Carol Zhou, Enbo Yang, Shu Xiao, Andrei G. Pakhomov

Bioelectrics Publications

A unique aspect of electrostimulation (ES) with nanosecond electric pulses (nsEP) is the inhibition of effects when the polarity is reversed. This bipolar cancellation feature makes bipolar nsEP less efficient at biostimulation than unipolar nsEP. We propose to minimize stimulation near pulse-delivering electrodes by applying bipolar nsEP, whereas the superposition of two phase-shifted bipolar nsEP from two independent sources yields a biologically-effective unipolar pulse remotely. This is accomplished by electrical compensation of all nsEP phases except the first one, resulting in the restoration of stimulation efficiency due to cancellation of bipolar cancellation (CANCAN-ES). We experimentally proved the CANCAN-ES paradigm by …

Intratumoral Delivery Of Plasmid Il12 Via Electroporation Leads To Regression Of Injected And Noninjected Tumors In Merkel Cell Carcinoma, Shailender Bhatia, Natalie V. Longino, Natalie J. Miller, Rima Kulikauskas, Jayasri G. Iyer, Dafina Ibrani, Astrid Blom, David R. Byrd, Upendra Parvathaneni, Christopher Twitty, Jean S. Campbell, Mai H. Le, Sharron Gargosky, Robert H. Pierce, Richard Heller, Adil Daud, Paul Nghiem

Bioelectrics Publications

Purpose: Interleukin-12 (IL12) promotes adaptive type I immunity and has demonstrated antitumor efficacy, but systemic administration leads to severe adverse events (AE), including death. This pilot trial investigated safety, efficacy, and immunologic activity of intratumoral delivery of IL12 plasmid DNA (tavo) via in vivo electroporation (i.t.-tavo-EP) in patients with Merkel cell carcinoma (MCC), an aggressive virus-associated skin cancer.

Experimental Design: Fifteen patients with MCC with superficial injectable tumor(s) received i.t.-tavo-EP on days 1, 5, and 8 of each cycle. Patients with locoregional MCC (cohort A, N = 3) received one cycle before definitive surgery in week 4. …

Spatiotemporal Modeling And Model Restructuration Approaches In Studies Of Intracellular Signalling Pathways, Md Shahinuzzaman

Doctoral Dissertations

"The main focus of the research is to understand the complex phenomena of cell transduction pathways and cell biology in a single cell. Mathematical modeling and experimental evaluation are widely used approaches for this kind of research. Firstly, A multiscale framework for protein-protein interaction has been established using Brownian dynamics algorithm. Sit specific feature, steric collision, diffusion, co-localization and complex formation with time and space has been included in this spatial modeling framework. By implementation of the time adaptive feature in this framework, the computation time reduces in an order of magnitude compared with traditional modeling framework. This multiscale Brownian …

Longitudinal Investigation Of Disparity Vergence In Young Adult Convergence Insufficiency Patients, Patrick C. Crincoli

Theses

Vergence is a form of eye movement in which the eyes move in opposite directions to minimize retinal disparity. It allows an object at different distances from a viewer to appear single during binocular vision by centering the image on the fovea of each retina. Convergence insufficiency (CI) is a binocular disfunction in which blurry and double vision is a symptom. Office-based Vergence/Accommodative Therapy (OBVAT) has been shown to be effective in treating CI. A randomized clinical trial was designed to study fifty participants with CI before and after therapy using randomized therapy treatment (active and placebo), standardized clinical definitions, …

Infection Control In Total Knee Replacement Prosthesis Using Nitric Oxide Releasing Biomaterials, Ashley Widing

Master's Theses

Replacing a weight-bearing joint is accompanied by long procedures, even longer recovery times, and intensive monitoring. This burden increases after infection; a complication requiring revision surgeries and therefore extensive additional recovery time. Infection can present itself at any time during the process, making it hard to predict. Currently, a two-part revision surgery is completed by removing all infected tissue and implanting an antibiotic spacer. The spacer fills the joint gap and is removed prior to the revision total knee replacement. A revision total knee replacement follows the same procedure as the initial surgery. Not only is this detrimental to patient …

Adenovirus Co-Opts Neutrophilic Inflammation In Order To Enhance Entry Into Epithelial Cells, James Matthew Readler

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Human adenoviruses (AdV) are double-stranded DNA viruses that can cause a range of diseases. While AdV respiratory infections are often mild and self-limited, severe manifestations such as fulminant pneumonia and acute respiratory distress syndrome (ARDS) are not uncommon. In order to identify potential modalities to treat AdV infections and potentially enhance the effectiveness of AdV vector delivery, a thorough mechanistic understanding of how AdV enters host cells is needed. Previous studies have revealed polarized epithelia, one of the primary targets for AdV respiratory infections, are highly resistant to AdV entry from the apical (luminal) surface. However, upon exposure to interleukin …

Quantification Of Optical Parameters Using Frequency Domain Functional Near-Infrared Spectroscopy (Fd-Fnirs), Christopher W. Davies

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Near infrared spectroscopy (NIRS) is a non-invasive technique that uses near-infrared light and hemoglobin as the contrast to characterize tissue optical properties, namely optical absorption [����] and scattering [����′ ]. Through this characterization, it is possible to obtain information about the tissue’s oxygenated (HbO) and deoxygenated (Hb) hemoglobin concentrations and oxygen saturation which are important physiological markers for diagnosis and intervention monitoring. Many commercial devices use a continuous wave approach (CW-fNIRS), a technique that simply relies on intensity changes from a constant power laser to determine only relative changes in oxygenation. This is a significant limitation for clinical settings where …

Rescue Of Als Protein Fus Toxicity By Taf, Elliott Hayden

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Amyotrophic Lateral Scleroses (ALS) is a neurodegenerative disease characterized by the degeneration of upper and lower motor neurons in the brain and spinal cord leading to progressive paralysis and ultimately death within 5 years of symptom onset. Only two drugs are approved by the Food and Drug Administration (FDA) for treating ALS that slow the disease progression by about 3 months. Mutations in the gene Fused in Sarcoma (FUS) cause inherited forms of ALS. FUS is a nuclear, multifunctional RNA-binding protein (RBP) involved in multiple RNA metabolic pathways. Mutations in FUS cause mislocalization of the protein from the nucleus to …

Modeling Runoff From Small Agricultural Watersheds In Eastern South Dakota, Bryce Siverling

Electronic Theses and Dissertations

The capability to be able to consistently and accurately model any problem has potential time and money savings. The present study aimed to determine if the Natural Resources Conservation Service’s Curve Number (CN) model or the more detailed Soil and Water Assessment Tool (SWAT) Model can consistently and accurately model runoff events from small agricultural fields in Eastern South Dakota. The overall goal was to better understand models used to predict runoff and determine if they can produce accurate estimates of runoff from the watersheds being studied. Runoff measurements were collected from an agricultural field located south of Coleman, South …

Celiac Disease: A Physiological Overview And Possible Future Work With Emphasis On Raman Spectroscopy, Katlyn Curtin Mehne

Wayne State University Theses

Celiac Disease is a condition whereby ingesting Gluten causes an autoimmune reaction in the intestines with adverse effects throughout the body. Although statistic analysis estimates that 1% of the general population is affected by this condition, the diagnosis and treatment research lacks in key areas of understanding. This is a summary of current research and physiological information on the impact of Celiac Disease. In the last twenty years, Raman Spectroscopy assisted in diagnosis and treatment of various other ailments. In this case, Raman Spectroscopy can be used to research immunological cells involved in Celiac Disease and further research.