Biomedical Engineering and Bioengineering Commons^™

Experiments And Modeling Of The Chemo-Mechanically Coupled Behavior Of Polymeric Gels, Nikola Bosnjak

Dissertations

Polymeric materials consist of mutually entangled or chemically crosslinked long njitmolecular chains which form a polymer network. Due to their molecular structure, the njitpolymeric materials are known to undergo large deformation in response to various njitenvironmental stimuli, such as temperature, chemical potential and light.

When a polymer network is exposed to a suitable chemical solvent, the solvent molecules are able to diffuse inside the network, causing it to undergo a large volumetric deformation, known as swelling. In addition to volumetric deformation, this process involves the chemical mixing of the polymer network and solvent molecules, and is typically environmentally responsive. A …

Blast Shock-Wave Characterization In Experimental Shock Tubes, Sudeepto Kahali

Dissertations

Blast-induced traumatic brain injuries have affected U.S. soldiers deployed for extended periods in the gulf and Afghanistan wars. To identify the biomechanical and biochemical mechanisms of injury, critical in the identification of diagnostic and therapeutic tools, compressed gas-driven shock tubes are used by investigators to study shockwave-animal specimen interactions and its biological consequences. However, shock tubes are designed and operated in a variety of geometry with a range of process parameters, and the quality of shock wave characteristics relevant to field conditions and therefore the study of blast-induced traumatic brain injuries suffered by soldiers is affected by those conditions. Lab-to-lab …

Cycloidal-Drive Joint Design For Wearable Exoskeletons, Abhi J. Rawal

Theses

This thesis's scope was to construct a highly back drivable and compact joint design that can be used for wearable exoskeletons specifically designed to assist with rehabilitation. A compact cycloidal gear transmission was designed to satisfy the compactness requirement while ensuring the minimum torque is required for backdriving the mechanism. The joint was designed based on the compact cycloidal drive specifications, and the backdrivability test was performed to measure the minimum torque required (1.20 Nm) to backdrive. The joint design incorporates various important features such as rotation lock feature to prevent hyperextension, a housing design that offers the benefit of …

The Mathematical Relationship Among Normal Patellar Dimensions, To Find The Pre-Diseased Patellar Thickness In Different Populations, Mahdie Kerdari, Shima Rastegar, Yasmeen Mustafa, Mohammadreza Abbassian, Armin Aalami Harandi, Mary M. Stahle, Robert Hube, Hamid Rs Hosseinzadeh

Henry M. Rowan College of Engineering Departmental Research

Whether resurfacing or not resurfacing the patella during total knee arthroplasty (TKA) still is a challenge to orthopedic surgeons. A significant reason for this controversy is the far from perfect outcomes of both techniques, resulting from inadequate knowledge of normal patellar dimensions in a diseased one. The primary purpose of the current study is to find the pre-diseased patellar dimensions and the ethnic differences in patellar dimensions. We measured the patella's dimensions on 927 normal young adult knee MRIs from seven different ethnicities. Besides comparing the dimensions between sexes, ages, and sides, we analyzed the differences among ethnic groups. The …

A Method For Classifying Ecg Signals With Different Possible States On A Multilayer Perceptron, Sherzod Nematov, Y Talatov

Technical science and innovation

To automatically determine the state of the cardiovascular system based on the recorded ECG signals, an artificial neural network is trained to classify signals into various possible states. At the same time, the parameters of heart rate variability (HRV) were extracted from the ECG signals and used as input functions for the neural network. HRV is the fluctuation in the time intervals between adjacent heartbeats. For this, the architecture of a neural network based on a multilayer perceptron and a method for obtaining the necessary parameters in the learning process have been developed, and the classification efficiency has been checked …

Microparticle Propulsion For In Vivo Navigation, Louis Rogowski

Mechanical Engineering Research Theses and Dissertations

Microscale propulsion impacts a diverse array of fields, with simplistic microrobots allowing for novel innovations in microscale surgery and drug delivery. Propulsion at the microscale is constrained by physics, with time-reversal and geometric symmetries limiting available propulsion mechanisms. However, certain fluid environments and surface coatings allow for the propulsion of microparticles through externally applied magnetic fields. Presented here is a detailed analysis of microparticles propelling using spontaneous symmetry breaking, flagella surface coatings, and multi-modal actuation mechanisms. Spontaneous symmetry breaking in nonlinearly viscoelastic fluids is presented for the first time in literature, with two equal and opposite propulsion states existing along …

Detection Of Nucleotides In Hydrated Ssdna Via 2-D H-Bn Nanopore With Ionic-Liquid/Salt-Water Interface, Jungsoo Lee

Multidisciplinary Studies Theses and Dissertations

Accomplishing slow translocation speed with high sensitivity has been the greatest mission for solid-state nanopore (SSN) to electrically detect nucleobases in single-stranded DNA (ssDNA). In this study, a method to detect nucleobases in ssDNA using a SSN is introduced by considerably slowing down the translocation speed and effectively increasing its sensitivity. The ultra-thin titanium dioxide (TiO₂) coated hexagonal boron nitride (h-BN) nanopore was fabricated, along with an ionic-liquid [bmim][PF₆]/2.0 M KCl aqueous (cis/trans) interfacial system, to increase both the spatial and the temporal resolutions. As the ssDNA molecules entered the nanopore, a …

Modeling Residence Time Distribution Of Chromatographic Perfusion Resin For Large Biopharmaceutical Molecules: A Computational Fluid Dynamic Study, Kevin Vehar

KGI Theses and Dissertations

The need for production processes of large biotherapeutic particles, such as virus-based particles and extracellular vesicles, has risen due to increased demand in the development of vaccinations, gene therapies, and cancer treatments. Liquid chromatography plays a significant role in the purification process and is routinely used with therapeutic protein production. However, performance with larger macromolecules is often inconsistent, and parameter estimation for process development can be extremely time- and resource-intensive. This thesis aimed to utilize advances in computational fluid dynamic (CFD) modeling to generate a first-principle model of the chromatographic process while minimizing model parameter estimation's physical resource demand. Specifically, …

Nanoscale Material Interaction With Multicellular 3d Cancer Culture, Emilie Darrigues

Theses and Dissertations

Pancreatic ductal adenocarcinoma (PDAC) is the most common form of pancreatic cancer, it is difficult to diagnose, often recurs, has high resistance to chemotherapies, and can be marked by advanced metastatic stages. There is an urgent need to fill the knowledge on nanoparticle penetration, diffusion, and cellular interaction in the context of adaptive PDAC tumor microenvironment to allow the development of new imaging and therapeutic supports. Traditionally, cancer-treating nanoparticles have been engineered and tested on 2D cell cultures, but several studies indicate that this in vitro model does not mimic tumor features closely enough. In contrast, 3D culture models offer …

Feasibility Of Continuous Fever Monitoring Using Wearable Devices, Benjamin L. Smarr, Kirstin Aschbacher, Sarah M. Fisher, Anoushka Chowdhary, Stephan Dilchert, Karena Puldon, Adam Rao, Frederick M. Hecht, Ashley E. Mason

Publications and Research

Elevated core temperature constitutes an important biomarker for COVID-19 infection; however, no standards currently exist to monitor fever using wearable peripheral temperature sensors. Evidence that sensors could be used to develop fever monitoring capabilities would enable large-scale health-monitoring research and provide high-temporal resolution data on fever responses across heterogeneous populations. We launched the TemPredict study in March of 2020 to capture continuous physiological data, including peripheral temperature, from a commercially available wearable device during the novel coronavirus pandemic. We coupled these data with symptom reports and COVID-19 diagnosis data. Here we report findings from the first 50 subjects who reported …

Functional Complementation Of The Ppgcn4 And Ppnhx2 Genes In Arabidopsis Thaliana To Study Salt Tolerance, Amanda Moravek

Fall Student Research Symposium 2020

Climate change induces unexpected weather and causes abiotic and biotic stresses in plants. These stresses negatively affect crop growth and production. Additionally, a steady increase in the world population has been leading to higher food demand. Therefore, the development of more stress-resilient crops is essential to combat these problems. One such stress is a salinity that affects crop production. Almond is a salt-sensitive crop, so there is a need to identify salt-tolerant almond rootstocks. AtGCN4 is a novel gene that was identified in Arabidopsis thaliana to play a significant role in host-pathogen interaction and drought tolerance when overexpressed. The preliminary …

Dogs In Ancient Egypt, Ella Olson

Fall Student Research Symposium 2020

The Ancient Egyptians are infamous for their love of cats, but what about dogs? It turns out that dogs were equally loved and praised in Egyptian culture. A large part of this comes from the religious significance of dogs- they are connected to the afterlife through Anubis, the god of the dead, and were thought to act as companions and guides to humans in the afterlife. Anubis is an interesting god to look at because he's one of the oldest and most respected gods. In today's terms, we refer to him as having a jackal head, but the Egyptians considered …

Framework For Water Management In The Food-Energy-Water (Few) Nexus In Mixed Land-Use Watersheds In Colombia, Camilo Torres, Margaret Gitau, Jaime Lara-Borrero, Diego Paredes-Cuervo

Purdue University Libraries Open Access Publishing Fund

The food-energy-water (FEW) nexus approach has emerged as an alternative for managing these resources more efficiently. Work from studies conducted in the FEW nexus in Latin America is scarce in the scholarly literature. This study aims to develop a framework for water management at the FEW Nexus, with a focus on Colombia. The study focuses on a typical mixed land-use watershed in the Andean region with specific objectives being to: (1) characterize the watershed with respect to land use, climate, water resources, and other factors pertinent to the nexus; (2) explore the relationship between factors in the FEW nexus that …

Array Modeling And Testing Of Fixed Owc Type Wave Energy Converters, Bret Bosma, Ted Brekken, Pedro Lomonaco, Bryony Dupont, Chris Sharp, Belinda Batten

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

If wave energy technology is to mature to commercial success, array optimization could play a key role in that process. This paper outlines physical and numerical modeling of an array of five oscillating water column wave energy converters. Numerical model simulations are compared with experimental tank test data for a non-optimal and optimal array layout. Results show a max increase of 12% in average power for regular waves, and 7% for irregular waves between the non-optimized and optimized layouts. The numerical model matches well under many conditions; however, improvement is needed to adjust for phase errors. This paper outlines the …

Peristaltic Flow In The Glymphatic System., Francesco Romanò, Vinod Suresh, Peter Galie, James B Grotberg

Henry M. Rowan College of Engineering Departmental Research

The flow inside the perivascular space (PVS) is modeled using a first-principles approach in order to investigate how the cerebrospinal fluid (CSF) enters the brain through a permeable layer of glial cells. Lubrication theory is employed to deal with the flow in the thin annular gap of the perivascular space between an impermeable artery and the brain tissue. The artery has an imposed peristaltic deformation and the deformable brain tissue is modeled by means of an elastic Hooke's law. The perivascular flow model is solved numerically, discovering that the peristaltic wave induces a steady streaming to/from the brain which strongly …

Improve The Prototype Of Low-Cost Near-Infrared Diffuse Optical Imaging System, Chen Xu, Mohammed Z. Shakil

Publications and Research

Diffuse Optical Tomography (DOT) and Optical Spectroscopy using near-infrared (NIR) diffused light has demonstrated great potential for the initial diagnosis of tumors and in the assessment of tumor vasculature response to neoadjuvant chemotherapy. The aims of this project are 1) to test the different types of LEDs in the near-infrared range, and design the driving circuit, and test the modulation of LEDs at different frequencies; 2) to test the APDs as a detector, and build the receiver system and compare efficiency with pre-built systems. In this project, we are focusing on creating a low-cost infrared transmission system for tumor and …

The Sars-Cov-2 Spike Protein Alters Barrier Function In 2d Static And 3d Microfluidic In-Vitro Models Of The Human Blood-Brain Barrier., Tetyana P Buzhdygan, Brandon J Deore, Abigail Baldwin-Leclair, Trent A Bullock, Hannah M Mcgary, Jana A Khan, Roshanak Razmpour, Jonathan F Hale, Peter Galie, Raghava Potula, Allison M Andrews, Servio H Ramirez

Henry M. Rowan College of Engineering Departmental Research

As researchers across the globe have focused their attention on understanding SARS-CoV-2, the picture that is emerging is that of a virus that has serious effects on the vasculature in multiple organ systems including the cerebral vasculature. Observed effects on the central nervous system include neurological symptoms (headache, nausea, dizziness), fatal microclot formation and in rare cases encephalitis. However, our understanding of how the virus causes these mild to severe neurological symptoms and how the cerebral vasculature is impacted remains unclear. Thus, the results presented in this report explored whether deleterious outcomes from the SARS-CoV-2 viral spike protein on primary …

Technology Aiding In Neonatal Lung Developmental Care, Megan Kirk

Undergraduate Honors Theses

In this paper, old as well as new technological findings to decrease premature infant mortality are reviewed. This paper discusses fetal development throughout pregnancy from conception to full-term status as well as fetal lung development specifically from conception until full-term status. Several ideas to rapidly develop and mature fetal lungs are discussed such as mothers ingesting artificial surfactant supplements, either independently or coupled with antenatal corticosteroids, as well as intra-amniotic instillation prior to 28 weeks gestational. Drawbacks regarding these two are mentioned as well such as the fetus’s lungs not being mature enough to use the artificial surfactant leading into …

Recombinant Piriform Spider Silk Production, Cole R. Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Spider silks are remarkable biomaterials, possessing a variety of desirable mechanical and material properties. Humans have long-sought to utilize these materials. Spiders produce a range of different types of silks for different purposes, such as web-building, egg casing, and prey swathing. Because spiders are difficult to farm, a synthetic biological approach must be used to produce silk-like proteins. This research is intended to improve the production of piriform, a specific type of spider silk. This was done with targeted-improvements to microbial metabolism and recovery methodologies. The methods developed in this research are useful for other types of silk production and …

Laboratory Scale Anaerobic Reactors For Bioenergy From Wastes: Materials, Mathematical Models, And Stoichiometry, Yehor Pererva

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Anaerobic processes are being explored for the production of bioenergy in the form of biomethane and biohydrogen in a "waste-to-value" approach that considers wastes as a resource. Laboratory scale Biomethane potential (BMP) tests and Up-Flow Anaerobic Sludge Blanket (UASB) reactors can be used to indicate the potential recovery of bioenergy from industry wastes. The goals of this research were to: (1) conduct a critical review of models for determining biomethane potential, (2) develop a theoretical relationship for stoichiometry for biomethane and biohydrogen from wastes, and (3) develop key design aspects for geometry and infrastructure, including gas-liquid-solid separation in laboratory UASB …