Biomedical Engineering and Bioengineering Commons^™

Volumetric Fluorescence Microscopy For High-Throughput And High-Sensitivity Imaging: From Single Molecules To Tissues, Le-Mei Wang

Graduate Studies Theses and Dissertations 2026

Fluorescence microscopy is an indispensable tool in the biological sciences, enabling researchers to investigate intricate subcellular structures, particularly for volumetric studies. However, conventional optical microscopy for volumetric imaging remains fundamentally constrained by imaging speed and throughput. To bypass traditional serial z-scanning, we introduce an axially scan-free method using a phase layer cake to modulate the system's point spread function. This approach projects volumetric information onto a 2D plane in a single shot, offering high flexibility in tuning axial depth alongside simultaneous multicolor imaging with high spatial resolution and sensitivity. This dissertation divides these technical advancements into cellular and tissue imaging …

Crashheart, Ellie Riekert, Kennady Skidmore, Madison Furino

Williams Honors College, Honors Research Projects

This project aims to develop an XR (extended reality) iPad training system to improve pediatric crash cart preparedness among nurses and clinical staff at Akron Children’s Hospital. In emergencies such as respiratory failure or shock, quick and accurate access to crash cart supplies is vital, yet opportunities for hands-on practice are limited. Current nurse training relies on PowerPoint instruction or costly physical simulations, reducing engagement and skill retention. Our interactive XR solution replicates the crash cart’s layout and contents, allowing users to practice identifying and retrieving critical items in a realistic environment without using physical supplies. The system will be …

Rapid Skin Stapler, Emma J. Patterson, Allison M. Milligan, Morgan E. Dunlap

Williams Honors College, Honors Research Projects

Severe burn injuries frequently require split-thickness skin grafts, with metal surgical staples serving as the current standard for fixation. While staples provide rapid and secure placement of graft material, they also introduce significant challenges. Removal is painful, time-consuming, and often requires sedation in pediatric patients, leading to added risk, resource strain, and poor patient experience. These drawbacks highlight the need for improved fixation methods that maintain graft stability while reducing procedural burden. This project applies the engineering design process to address these challenges through identification of user needs, translation into design inputs, risk assessment, and development of a prototype aimed …

Breech Baby, Madison Kaufman, Cassidy Allen, Lilian Mills

Williams Honors College, Honors Research Projects

Our project aims to develop a non-invasive device to assist in turning breech babies to a head down position before delivery. Pregnant women facing breech presentations often need safe and effective solutions, yet current options like external cephalic version (ECV), maternal positioning techniques, and cesarean sections can present challenges. Key barriers include variability in maternal and fetal anatomy, patient comfort, compliance, and training for healthcare providers, along with cost and insurance concerns.

Our innovative device will address these issues by providing clear guidance and support throughout the process. It will prioritize minimizing discomfort and risk, ensuring adaptability for various stages …

Integrated Optical Probes For Confocal Scanning Imaging And Adjustable Coherent-Gated Dynamic Sensing, Yonglin Huang

Graduate Studies Theses and Dissertations 2026

Optics and photonics have been one of the most important sciences and technologies that impact modern human life in a big way. For example, fiber-optics for communications and artificial intelligence. Optical probes are critical components for optical imaging and optical sensing technologies that have been actively researched and developed in the past decades. Advanced fiber-optic sensor probes with smaller size, better performance, lower noise, higher photon efficiency, rapid sensing time, and lower cost are needed in many applications, such as nanoscale material science, chemistry, and biomedical fields, etc.  In this project, new fiber-optic sensor probe technologies and integrated micro-optic devices …

Synthesis And Characterization Of Glass-Ceramic Nanoparticles And Nanobubbles For Integration Into Bone Regenerative Biomaterials, Sun Latt

Honors Undergraduate Theses

Osteoporosis affects over 200 million individuals worldwide and is characterized by an imbalance between osteoclastic bone resorption and osteoblastic bone formation, leading to fragility fractures that significantly diminish quality of life. Current clinical bone cements, such as poly(methyl methacrylate), are limited by poor osseointegration, toxic monomers, and an inability to address the underlying pathophysiology of osteoporosis. This work presents the synthesis, characterization, and systematic in vitro evaluation of a sol-gel-derived, magnesium-incorporated glass-ceramic (GC) nanoparticle within the CaO-MgO-SiO₂ system for incorporation into a novel hydrogel bone cement and bioresorbable magnesium implants. XRD confirmed a biphasic amorphous-crystalline diopside composition, and TEM …

The Development And Implementation Of A Computational Framework To Simulate Patellofemoral Joint Mechanics, Mahmood Rashedi

Master’s Theses

Anterior knee pain affects approximately 25% of adults and is associated with altered joint mechanics. Patellofemoral joint (PFJ) models vary widely in complexity, with no established gold standard. Musculoskeletal models efficiently estimate joint loading but do not capture tissue-level mechanics, whereas finite element (FE) models provide tissue-level predictions at greater computational cost. This thesis developed a workflow to evaluate relationships between musculoskeletal model-predicted PFJ loads and FE-predicted cartilage mechanics, examined the impact of altered quadriceps activation, and introduced improved computational models.

Marker trajectories and ground reaction forces were collected from four adults (2 male, 2 female) during walking and squatting. …

Monitoring Eye Movements To Assess The Impact Of Sensory Feedback On Cognitive Workload During Myoelectric Prosthetic Hand Use, Naomi Tchoungang, Hailey Dirrigl, Justin Asbee

2026 Research Poster Competition

Modern prosthetic hands allow users to grasp and manipulate objects, but many users must constantly watch the device to complete even simple tasks. This heavy reliance on vision increases cognitive effort, slows movement, and makes prosthetic use feel less natural. An important question in prosthetics research is whether providing sensory feedback (enabling users to feel what the prosthetic hand is doing) can reduce this cognitive load and improve performance. In this study, sensory feedback refers to a system that provides clear tactile cues to the user upon contact between the prosthetic hand and an object, helping replace information normally felt …

Assessing Mesenchymal Stem Cell Phenotype After Heparan Sulfate Supplementation, Isani Patel

2026 Research Poster Competition

Multiple sclerosis is an autoimmune disease that damages the protective coating of nerves, often causing lasting neurological symptoms. Current treatments slow disease activity but rarely repair existing damage. In response to this, a novel treatment option has emerged: mesenchymal stem cell (MSC) therapy. MSCs are cells found in different adult tissues like bone marrow, and they have the potential to release repair-supporting signals and moderate inflammation. Nevertheless, results in a clinical setting have been inconsistent due to variations in cell potency. As a potential strategy to enhance MSC therapeutic potential, the addition of heparan sulfate, a naturally occurring signaling molecule, …

Cardiovascular Monitoring For Maternal Health Using Wearable Devices, Daniela Alejandra Vasquez

2026 Research Poster Competition

Cardiovascular disease represents the leading cause of pregnancy-related mortality in the United States, accounting for over 30% of maternal deaths, with preeclampsia and hypertensive disorders contributing significantly to this burden. In Arkansas, maternal mortality rates reach 41.9 deaths per 100,000 live births. Current prenatal care relies on intermittent office-based blood pressure measurements conducted during 12-14 clinical visits, may not capture critical cardiovascular changes in a timely manner. Preeclampsia is characterized by new-onset hypertension after 20 weeks of gestation, affects 3-7% of pregnancies, and requires early detection to enable timely intervention. The limited accessibility of ambulatory blood pressure monitoring in rural …

Uav-Based Sensor Integration For In-Situ Lake Water Quality Monitoring, Alec Campbell

2026 Research Poster Competition

Current water quality assessment methods rely heavily on manual sampling and laboratory analysis, which are time-consuming and difficult to perform in remote or hazardous environments. In surface water monitoring, spatial and temporal resolution is often constrained by labor, access, and logistical limitations of manual sampling programs. This research aims to develop a drone-based system for real-time, in situ lake water quality monitoring. To address these challenges, the project focuses on integrating multiple water quality sensors, including oxidation-reduction potential (ORP), pH, electrical conductivity (EC), turbidity, and a multispectral spectrometer, into a compact, UAV-compatible design capable of collecting spatially referenced data. Methods: …

Enhancing Prosthetic Hand Training Through A Virtual Reality–Based Framework Integrated With The Coapt Control System., Iruoghene Ojoh

2026 Research Poster Competition

Enhancing Prosthetic Hand Training Through a Virtual Reality–Based Framework  Integrated with the Coapt Control System. Ojoh, I.1,2, Shell, A.1,  Asbee, J.1, Aguilar, D.1 ,Abbas, J.1,2,   1Institute for Integrative and Innovative Research, University of Arkansas, Fayetteville, AR;  2Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR  Introduction: Advances in upper-limb prosthetic hardware have greatly improved strength, durability, and mechanical performance; however, achieving intuitive control of EMG-based myoelectric prosthetic hands remains a significant challenge for individuals with trans-radial amputations. These devices rely on electromyography (EMG) signals generated by residual forearm muscles, which require consistent and well-coordinated muscle activation. Many users experience difficulty …

Testing The Effects Of Mechanical Stimulation On Mesenchymal Stem Cell Cultures, Ella Collis

2026 Research Poster Competition

Mesenchymal stem cells (MSCs) are a promising treatment option for a variety of diseases due to their ability to migrate to far-off sites, modulate inflammatory immune responses, secrete factors that aid in tissue healing, and their capacity to become several cell types. These cells can be found and isolated from various body tissues including bone marrow and adipose tissue, even so the number of cells obtained from these tissues is minimal compared to the amounts needed for their transplantation in cell therapy treatments. Therefore, their culture in plastic dishes is required to increase the number of cells and obtain the …

Monitoring Streambank Morphology Using Iphone Lidar Technology, Lily C. Stitt

2026 Research Poster Competition

Monitoring stream morphology is crucial for maintaining the health and safety of fluvial environments, particularly as these naturally dynamic systems are shifted out of equilibrium due to climate and land-use change. Quantifying these changes in stream geometry is useful for infrastructure design, water quality assessment, water resource management, and stream restoration, among other applications. Traditional stream surveying methods contain limitations that inhibit affordable and accessible stream surveying needed for evaluating fluvial systems with high accuracy and large data samples. In recent years, several advancements have been made in the use of Light Detection and Ranging (LiDAR) technology as an alternative …

Developing A Cpp-Conjugated Cas9 Library For Efficient Non-Viral Delivery To The Dmd Gene, Amie Donathan

2026 Research Poster Competition

Duchenne muscular dystrophy, or DMD, is a genetic neuromuscular disorder caused by the mutation of the DMD gene, resulting in alterations in the functionality or production of dystrophin, a protein essential to maintain muscle fiber integrity and strength. This mutation leads to progressive muscle degeneration, causing motor loss, cardiopulmonary failure, and ultimately, premature death. Gene therapies using CRISPR aim to replace the mutated gene to provide long-term correction. Although this has promise, CRISPR-based therapies use a viral vector to deliver, which presents notable challenges, such as immune problems and liver toxicity. A non-viral delivery method is a viable approach, but …

Empirical Dynamical Modeling Of Cortico-Hippocampal Information Transfer During Memory Encoding, Sam Y. Cole

Theses and Dissertations

Understanding how brain dynamics support successful memory formation remains a central challenge in neuroscience. This dissertation applies tools from empirical dynamical systems theory to investigate the neural mechanisms underlying episodic encoding, with a focus on cortico-hippocampal interactions. Using stereoelectroencephalography (sEEG) data recorded during a delayed free recall of word lists task, this work reveals nonlinear, state-dependent shifts in directed information flow between cortex and hippocampus that distinguish successful from unsuccessful encoding. To further characterize these dynamics, state-space trajectories are reconstructed using time-delay embedding and recurrence-based methods, and features derived from these trajectories are used to classify memory outcomes. By integrating …

Regulation Of Tgf-Β1–Induced Epithelial–Mesenchymal Transition By Integrin Inhibition, Wesam Elhawabri

Theses and Dissertations

Epithelial–mesenchymal transition (EMT) is a critical process underlying cancer progression, fibrosis, and tissue remodeling, characterized by coordinated changes in cell morphology, nuclear structure, and extracellular matrix (ECM) organization. Transforming growth factor beta 1 (TGF-β1) is a primary inducer of EMT; however, the extent to which integrin-mediated signaling and ECM remodeling regulate these structural changes remains incompletely understood. In this study, we quantitatively investigated EMT-associated remodeling in A549 lung carcinoma cells and MCF10A epithelial cells following TGF-β1 stimulation. Cells were cultured on ECM-coated substrates and treated with TGF-β1 in the presence or absence of integrin-targeting inhibitors (ATN-161, 28-G11, and STX-100). Im …

De Novo Protein Binding To Zinc Oxide Through Biomineralization Pathways, Jean-Mark A. Francis

Theses and Dissertations

De novo proteins are structurally distinct from proteins found in nature and thus capable of having their amino acid sequence modified to accomplish tasks such as increasing protein-nanoparticle binding without unfolding or decomposing. Zinc Oxide nanoparticles are functionally distinct from their bulk counterparts and are widely used as semiconductors in a variety of fields such as medicine and agriculture. With demand for these nanoparticles increasing, environmentally sustainable methods of Zinc Oxide nanoparticle synthesis are being investigated as an eco-friendly alternative to currently utilized but environmentally hazardous chemical and physical techniques. This research investigates the binding characteristics between Zinc Oxide nanoparticles …

Bioprinted Scaffolds For Investigating Fibroblast Alignment, Lillian Thro, Daeha Joung Ph.D.

Undergraduate Research Posters

Fibroblasts play a vital role in tissue repair, ECM remodeling, and maintaining structural integrity in human tissues. However, their behavior in engineered 3D microenvironments remains poorly understood. Traditional 2D cultures fail to capture the complex cell-cell and cell-matrix interactions that govern fibroblast alignment, migration, and proliferation in vivo. To address this gap, this project uses bioprinted scaffolds that mimic native ECM structures and provide a platform for studying fibroblast behavior in controlled microenvironments. The project focuses on varying scaffold channel properties—such as width, depth, and geometry—to investigate how physical cues influence fibroblast organization and collective migration. A biodegradable gelatin-based ECM …

Evaluating The Impact Of Cognitive Distraction On Spaceflight-Relevant Task Performance Using Surface Electromyography And Motion Capture, Allyson K. Mitchell

UNF Graduate Theses and Dissertations

Cognitive distraction poses a risk to astronaut performance during complex, multitasking operations in spaceflight environments. This study examined the effects of cognitive load on neuromuscular coordination and task execution using surface electromyography (sEMG) and motion capture. Thirteen participants performed spaceflight-relevant tasks under undistracted and distracted conditions, with distraction induced through verbal questioning. EMG signals from eight upper-extremity muscles were processed using envelope filtering, peak normalization, and time normalization to enable inter-subject comparison, and group-level mean activation with standard deviation was analyzed. While overall muscle activation was similar between conditions, phase-dependent differences were observed, with undistracted trials showing higher activation during …