Biomedical Engineering and Bioengineering Commons^™

Evaluating The Structural And Functional Consequences Of Traumatic Joint Injury And Their Relation To Nf-Κb In A Non-Invasive Model Of Post-Traumatic Osteoarthritis, Ian Matthew Berke

McKelvey School of Engineering Graduate Student Theses & Dissertations

Post-traumatic osteoarthritis (PTOA) is a painful and debilitating disease of the synovial joint, characterized by degenerative changes to various joint tissues following traumatic joint injury. While several risk factors have been identified in the symptomatic progression of PTOA following injury, inflammation and NF-κB mediated changes are believed to significantly contribute to symptomatic joint dysfunction and pain. However, the temporal presentation of these pro-inflammatory signals following clinically relevant injury and their relationship to the development of symptomatic disease have not been thoroughly investigated. Therefore, there exists a critical need to better understand how these early inflammatory events following injury may contribute …

Neural Coding And Organization Principles In The Drosophila Olfactory System, Haoyang Rong

McKelvey School of Engineering Graduate Student Theses & Dissertations

Sensory systems receive and process external stimuli to allow an organism to perceive and react to the environment. How is sensory information subsequently represented, transformed, and interpreted in the neural system? In this dissertation, I have investigated this fundamental question using the fruit fly (Drosophila melanogaster) olfactory system.Chemical cues are transduced into neural signals in the insect antenna by the olfactory receptor neurons (ORNs). The ORNs send their axons to the antennal lobe (AL), with each ORN type innervating a specific neuropil (glomerulus), where they synapse onto excitatory and inhibitory projection neurons (ePNs and iPNs). The ePNs project their axons …

Focused Ultrasound-Mediated Drug Delivery To The Brainstem, Dezhuang Ye

McKelvey School of Engineering Graduate Student Theses & Dissertations

Brainstem gliomas are tumors that occur in the brainstem, the brain region that connects the brain to the spinal cord and controls vital body functions. The critical anatomic location of the brainstem precludes surgical intervention and limits the use of invasive therapeutic techniques. Moreover, the frequently intact blood-brain barrier (BBB) of most brainstem gliomas prevents therapeutic agents from reaching the diseased site. The currently available techniques for brain drug delivery are either invasive (e.g., convection-enhanced delivery) or lack targeting to the diseased site (e.g., intranasal brain drug delivery). Novel techniques that can noninvasively overcome the BBB are critically needed for …

Differentiating Human Embryonic Stem Cells In Micropatterns To Study Cell Fate Specification And Morphogenetic Events During Gastrulation, Kyaw Thu Minn

McKelvey School of Engineering Graduate Student Theses & Dissertations

During mammalian embryogenesis, the first major lineage segregation occurs when embryonic epiblast, and extraembryonic trophectoderm and hypoblast arise in the blastocyst. In the next fundamental and conserved phase of animal embryogenesis known as gastrulation, extraembryonic cells provide signals to epiblast to instruct embryonic patterning, and epiblast gives rise to germ layers ectoderm, mesoderm, and endoderm, that will establish all embryonic tissues. Proper specification and morphogenesis of germ layers during gastrulation is vital for correct embryonic development. Due to ethical and legal restrictions limiting human embryo studies, human gastrulation is poorly understood. Our knowledge of human gastrulation has largely been derived …

Theory, Design And Implementation Of Energy-Efficient Biotelemetry Using Ultrasound Imaging, Sri Harsha Kondapalli

McKelvey School of Engineering Graduate Student Theses & Dissertations

This dissertation investigates the fundamental limits of energy dissipation in establishing a communication link with implantable medical devices using ultrasound imaging-based biotelemetry.

Ultrasound imaging technology has undergone a revolution during the last decade due to two primary innovations: advances in ultrasonic transducers that can operate over a broad range of frequencies and progresses in high-speed, high-resolution analog-to-digital converters and signal processors. Existing clinical and FDA approved bench-top ultrasound systems cangenerate real-time high-resolution images at frame rates as high as 10000 frames per second. On the other end of the spectrum, portable and hand-held ultrasound systems can generate high-speed real-time scans, …

Evaluation Of Anticipatory Postural Adjustment Before Quantified Weight Shifting—System Development And Reliability Test, Jiunn-Woei Liaw, Rou-Shayn Chen, Vincent Chiun-Fan Chen, Yan-Ru Wang, Hsiao-Lung Chan, Ya-Ju Chang

Engineering Science Faculty Publications

Anticipatory postural adjustment (APA) existed before a self-induced perturbation is an important motor control skill for balance and gait initiation, but cannot be easily monitored. During proactive balance test, a self-initiated weight shifting is produced. This might be an optimal paradigm for APA measurement. The purpose of this study was to investigate if APAs existed in the proactive balance test which consists of quantifiable weight shifting. The feature and reliability of the APAs were also evaluated. We firstly built a proactive balance test program on the commercially available Wii balance board. The program could generate adjustable target direction and distance …

Gas Measurement Device, Megan C. Frost, Weilue He

Michigan Tech Patents

A device including a first chamber, a second chamber, and a membrane permeable to neutral gases but impermeable to water that is positioned between the first chamber and the second chamber. The membrane includes a first layer including PVDF and PDMS, and the PVDF has a plurality of pores at least partially filled with at least some of the PDMS.

Liposomal Delivery Of Remdesivir For Localized And Targeted Treatment Of Covid-19, Anupama Melam

McKelvey School of Engineering Graduate Student Theses & Dissertations

Liposomal delivery of Remdesivir for localized and targeted treatment of COVID-19

COVID-19 is a serious, and in many cases lethal, disease that is caused by infection of the upper respiratory tract by the novel betacoronavirus, SARS-Cov-2 virus. This disease has a very high mortality rate and has affected the world in a global pandemic. SARS-Cov-2 binds to the ACE2 receptor via the receptor-binding domain (RBD) in the S protein. After this, the virus fuses with the cell membrane by the formation of a six-helix bundle. Thus, the S protein plays a major role in ensuring that the virus attaches to …

Special Issue On Professor John M. Tarbell’S Contribution To Cardiovascular Engineering, Hanjoong Jo, Keefe Manning, John M. Tarbell

Publications and Research

No abstract provided.

Electrical Double Layer In Nanopores For Detection And Identification Of Molecules And Submolecular Units, Guigen Zhang, Samuel Bearden

Biomedical Engineering Faculty Patents

Systems for detecting analytes in electrical double layer nanopore devices and methods of use are provided.

In Vivo Optical Metabolic Imaging Of Long-Chain Fatty Acid Uptake In Orthotopic Models Of Triple-Negative Breast Cancer, Megan C. Madonna, Joy E. Duer, Joyce V. Lee, Jeremy Williams, Baris Avsaroglu, Caigang Zhu, Riley Deutsch, Roujia Wang, Brian T. Crouch, Matthew D. Hirschey, Andrei Goga, Nirmala Ramanujam

Biomedical Engineering Faculty Publications

Targeting a tumor’s metabolic dependencies is a clinically actionable therapeutic approach; however, identifying subtypes of tumors likely to respond remains difficult. The use of lipids as a nutrient source is of particular importance, especially in breast cancer. Imaging techniques offer the opportunity to quantify nutrient use in preclinical tumor models to guide development of new drugs that restrict uptake or utilization of these nutrients. We describe a fast and dynamic approach to image fatty acid uptake in vivo and demonstrate its relevance to study both tumor metabolic reprogramming directly, as well as the effectiveness of drugs targeting lipid metabolism. Specifically, …

Quantitative Assessment Of Intra- And Inter-Modality Deformable Image Registration Of The Heart, Left Ventricle, And Thoracic Aorta On Longitudinal 4d-Ct And Mr Images, Alireza Omidi, Elisabeth Weiss, John S. Wilson, Mihaela Rosu-Bubulac

Biomedical Engineering Publications

Purpose

Magnetic resonance imaging (MRI)-based investigations into radiotherapy (RT)-induced cardiotoxicity require reliable registrations of magnetic resonance (MR) imaging to planning computed tomography (CT) for correlation to regional dose. In this study, the accuracy of intra- and inter-modality deformable image registration (DIR) of longitudinal four-dimensional CT (4D-CT) and MR images were evaluated for heart, left ventricle (LV), and thoracic aorta (TA).

Methods and materials

Non-cardiac-gated 4D-CT and T1 volumetric interpolated breath-hold examination (T1-VIBE) MRI datasets from five lung cancer patients were obtained at two breathing phases (inspiration/expiration) and two time points (before treatment and 5 weeks after initiating RT). Heart, LV, …

Nmr Metabolomics For Optimizing Cell-Free Protein Synthesis, Angela M. Campo

Browse all Theses and Dissertations

Cell-Free Protein Synthesis (CFPS) has been utilized by biochemists to produce a variety of chemicals and therapeutics. While CFPS has spawned research in the biochemistry and medical communities, there are still unknown issues with interlaboratory variability with the technique. This work explored the black box nature of CFPS reactions by analyzing the CFPS reactions in situ with Nuclear Magnetic Resonance (NMR) spectroscopy. Aim 1 developed the protocol for conducting NMR experiments on E. coli cell-free reactions as well as a data analysis pipeline. This was accomplished with 1H NMR, capturing metabolite changes over time. The 1D NOESY experiment proved to …

Multifactorial Media Analysis Via Design Of Experiment For Type Ii Collagen In Primary Rabbit Chondrocytes, Javier A. Velez Toro

Honors Undergraduate Theses

Osteoarthritis is a prevalent disease that affects the articular cartilage of the joints. Millions of people suffer worldwide and it is a major cause of disability in the United States. Current research for treatments of osteoarthritis are studying tissue-engineered cartilage in vitro generated by articular chondrocytes. A challenge faced in vitro for cartilage tissue engineering is the failure of chondrocytes to produce adequate expression of type II collagen. Surprisingly, the media commonly used in vitro lacks 14 vitamins and minerals present in the physiological environment of chondrocytes. Therefore, studying the interactions between micronutrients and chondrocytes may help in potentially increasing …

Correlating With Local Variation In Collagen Level And Mechanical Properties Of The Heart Tissues, Talayeh Tavangar

Electronic Theses and Dissertations

The determination of the myocardium's tissue properties is essential in constructing finite element (FE) models of the heart. To obtain accurate results, especially for functionally modeling the heart, we must determine the tissue properties in-vivo. The calculation of the heart tissue properties remains a challenging area as it is categorized as a heterogeneous, anisotropic, nonlinear soft tissue that undergoes large deformation. In this work, we tried to introduce and evaluated a finite element method to determine the mechanical properties of the cardiac tissue. The introduced method combined a finite element modeling with the experimentally obtained images from the left and …

The Use Of Tissue Engineering To Fabricate Perfusable 3d Brain Microvessels, Kalpani N Udeni Galpayage Dona, Jonathan Franklin Hale, Tobi Salako, Akanksha Anandanatarajan, Kiet A Tran, Brandon J Deore, Peter Galie, Servio Heybert Ramirez, Allison Michelle Andrews

Henry M. Rowan College of Engineering Departmental Research

Tissue engineering of the blood-brain barrier (BBB) in vitro has been rapidly expanding to address the challenges of mimicking the native structure and function of the BBB. Most of these models utilize 2D conventional microfluidic techniques. However, 3D microvascular models offer the potential to more closely recapitulate the cytoarchitecture and multicellular arrangement of in vivo microvasculature, and also can recreate branching and network topologies of the vascular bed. In this perspective, we discuss current 3D brain microvessel modeling techniques including templating, printing, and self-assembling capillary networks. Furthermore, we address the use of biological matrices and fluid dynamics. Finally, key challenges …

Data-Driven Design Of Energy-Shaping Controllers For Swing-Up Control Of Underactuated Robots, Wankun Sirichotiyakul, Aykut C. Satici

Mechanical and Biomedical Engineering Faculty Publications and Presentations

We propose a novel data-driven procedure to train a neural network for the swing-up control of underactuated robotic systems. Our approach is inspired by several recent developments ranging from nonlinear control theory to machine learning. We embed a neural network indirectly into the equations of motion of the robotic manipulator as its control input. Using familiar results from passivity-based and energy-shaping control literature, this control function is determined by the appropriate gradients of a neural network, acting as an energy-like (Lyapunov) function. We encode the task of swinging-up robotic systems through the use of transverse coordinates and goal sets; which …

A Linear Predictive Coding Filtering Method For Time-Resolved Morphology Of Eeg Activity, Jin Xu, Mark Davis, Ruairí De Fréin

Conference papers

This paper introduces a new time-resolved spectral analysis method based on the Linear Prediction Coding (LPC) method that is particularly suited to the study of the dynamics of EEG (Electroencephalography) activity. The spectral dynamics of EEG signals can be challenging to analyse as they contain multiple frequency components and are often corrupted by noise. The LPC Filtering (LPCF) method described here processes the LPC poles to generate a series of reduced-order filter transform functions which can accurately estimate the dominant frequencies. The LPCF method is a parameterized time-frequency method that is suitable for identifying the dominant frequencies of multiple-component signals …

Wearable Knee Assistive Devices For Kneeling Tasks In Construction, Siyu Chen, Duncan Stevenson, Shuangyue Yu, Monika Mioskowska, Jingang Yi, Hao Su, Mitja Trkov

Henry M. Rowan College of Engineering Departmental Research

Construction workers regularly perform tasks that require kneeling, crawling, and squatting. Working in awkward kneeling postures for prolonged time periods can lead to knee pain, injuries, and osteoarthritis. In this paper, we present lightweight, wearable sensing and knee assistive devices for construction workers during kneeling and squatting tasks. Analysis of kneeling on level and slopped surfaces (0, 10, 20 degs) is performed for single- and double-leg kneeling tasks. Measurements from the integrated inertial measurement units are used for real-time gait detection and lower-limb pose estimation. Detected gait events and pose estimation are used to control the assistive knee-joint torque provided …

Ionizable Lipid Nanoparticles For In Utero Mrna Delivery., Rachel S. Riley, Meghana V Kashyap, Margaret M Billingsley, Brandon White, Mohamad-Gabriel Alameh, Sourav K Bose, Philip W Zoltick, Hiaying Li, Rui Zhang, Andrew Y Cheng, Drew Weissman, William H Peranteau, Michael J Mitchell

Henry M. Rowan College of Engineering Departmental Research

Clinical advances enable the prenatal diagnosis of genetic diseases that are candidates for gene and enzyme therapies such as messenger RNA (mRNA)-mediated protein replacement. Prenatal mRNA therapies can treat disease before the onset of irreversible pathology with high therapeutic efficacy and safety due to the small fetal size, immature immune system, and abundance of progenitor cells. However, the development of nonviral platforms for prenatal delivery is nascent. We developed a library of ionizable lipid nanoparticles (LNPs) for in utero mRNA delivery to mouse fetuses. We screened LNPs for luciferase mRNA delivery and identified formulations that accumulate within fetal livers, lungs, …