Biomedical Engineering and Bioengineering Commons^™

Low-Intensity Ultrasonic Neuromodulation Of The Rat Hippocampus, Duc Nguyen

Dissertations and Theses

Techniques to non-invasively modulate brain activity are important for mapping human brain circuits, and also for the treatment of a host of neurological and psychiatric disorders marked by aberrant brain activity. Though a wide range of techniques for non-invasive neuromodulation have been proposed, the conventional approaches suffer from significant limitations. Most notably, focal stimulation of deep brain regions is presently only possible with invasive optogenetic and chemogenetic approaches that require craniotomies and genetic access to the brain.

Transcranial focused ultrasound stimulation (tFUS) possesses many of the characteristics desirable from a neuromodulation approach: non-invasiveness, a spatial resolution in the order of …

Photoacoustic Imaging For Image-Guided Interventions, Samuel John

Wayne State University Dissertations

Image-guided surgeries and therapeutic procedures utilize imaging modalities to enhance the surgeon’s capability of visualizing and guiding the medical instruments within the body. One such application is the endovenous laser ablation (EVLA), which utilizes an ultrasound (US)-guided catheter carrying high power continuous wave (CW) energy for treating varicose veins. Varicose veins, a condition defined by venous insufficiency, affects nearly 40 million people in the United States annually. In EVLA procedures, US limitations including angular dependency, poor contrast-to-noise ratio, poor signal-to-noise ratio, and artifacts limit the ability of the surgeon to detect the catheter tip within the vein. Furthermore, the absence …

การประยุกต์ใช้คิวซีเอ็มเซนเซอร์ในการวิเคราะห์อันตรกิริยาเพื่อการตรวจวัด Aggregatibacter Actinomycetemcomitans, สาธิต รอดภักดีกุล

Chulalongkorn University Theses and Dissertations (Chula ETD)

งานวิจัยนี้มีวัตถุประสงค์เพื่อศึกษาการประยุกต์ใช้ไบโอเซนเซอร์ชนิดควอตซ์คริสตัลไมโครบาลานซ์ (quartz crystal microbalance, QCM) ในการวิเคราะห์อันตรกิริยาระหว่างแบคทีเรียก่อโรคปริทันต์ชนิด Aggregatibacter actinomycetemcomitans กับโมโนโคลนอลแอนติบอดีที่จำเพาะเพื่อการตรวจวัดแบคทีเรียก่อโรคปริทันต์ ไบโอเซนเซอร์สำหรับการตรวจวัดถูกสร้างขึ้นโดยใช้คิวซีเอ็มเซนเซอร์ ชนิด 30 เมกะเฮิรตซ์ ที่ผ่านการปรับสภาพพื้นผิวอิเล็กโทรดทองด้วย 11-mercaptoundecanoic acid (11-MUA) และได้ประเมินความหนาแน่นการเรียงตัวของชั้น 11-MUA ด้วยเทคนิคไซคลิกโวลแทมเมตรี จากนั้นทำการตรึงโมโนโคลนอลแอนติบอดีชนิด Anti-A. actinomycetemcomitans กับ 11-MUA โดยใช้ 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) เพื่อใช้แอนติบอดีทำหน้าที่เป็นสารรู้จำทางชีวภาพ (biorecognition element) สำหรับการตรวจวัด A. actinomycetemcomitans การวิเคราะห์อันตรกิริยาระหว่างแบคทีเรียกับแอนติบอดีชนิด anti-A. actinomycetemcomitans ทำด้วยกัน 4 รูปแบบ ได้แก่ รูปแบบที่ 1 ใช้ข้อมูลการเปลี่ยนแปลงความถี่ (∆F) ซึ่งผลการศึกษาบ่งชี้ว่าสามารถใช้ข้อมูล ∆F ในการจำแนกความสามารถในการจับกันระหว่างแอนติบอดีและเซลล์แบคทีเรียชนิดต่าง ๆ ได้ โดยคู่แอนติบอดีและเซลล์แบคทีเรียที่มีความจำเพาะต่อกันมีรูปแบบ ∆F ที่แสดงให้เห็นแนวโน้มการเปลี่ยนแปลงของ ∆F ที่ขึ้นกับความเข้มข้น รูปแบบที่ 2 ใช้ค่าอนุพันธ์อันดับหนึ่งของการเปลี่ยนแปลงความถี่ (dF/dT) ซึ่งผลการศึกษาบ่งชี้ว่าสามารถใช้วิธีนี้จำแนกความจำเพาะของแอนติบอดีชนิด Anti-A. actinomycetemcomitans กับแบคทีเรียต่างชนิดได้ โดยเมื่อเกิดการจับกันระหว่างแอนติบอดีและเซลล์ที่มีความจำเพาะกัน ค่า dF/dT จะเปลี่ยนแปลงเป็นลบ และค่า dF/dT แสดงให้เห็นแนวโน้มการเปลี่ยนแปลงเป็นรูปแบบที่ขึ้นกับความเข้มข้นเช่นเดียวกับข้อมูล ∆F รูปแบบที่ 3 ใช้ข้อมูลเวลาการตอบสนอง (τ) ซึ่งผลการศึกษาบ่งชี้ว่าการจับกันระหว่างแอนติบอดีกับเซลล์ A. actinomycetemcomitans ที่ความเข้มข้นสูงสุด (1.16 × 108 เซลล์/มิลลิลิตร) ให้ค่า τ เฉลี่ยเพียง 143 วินาที ทำให้ทราบผลลัพธ์ของการจับกันระหว่างแอนติบอดีกับเซลล์ A. actinomycetemcomitans รวดเร็วกว่าการติดตามจากข้อมูล ∆F ถึง 3 เท่า รูปแบบที่ 4 ใช้ข้อมูลความชันของการตอบสนอง …

Type-B Energetic Processes And Their Associated Scientific Implications, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

Recently, our work has identified two thermodynamically distinct types (A and B) of energetic processes naturally occurring on Earth. Type-A energy processes such as the classical heat engines, ATP hydrolysis, and many of the known chemical, electrical, and mechanical processes apparently follow well the second law of thermodynamics; Type-B energy processes, for example, the newly discovered thermotropic function that isothermally utilizes environmental heat energy to do useful work in driving ATP synthesis, follow the first law of thermodynamics (conservation of mass and energy) but do not have to be constrained by the second law, owing to its special asymmetric functions. …

The Hitran2020 Molecular Spectroscopic Database, I. E. Gordon, L. S. Rothman, R. J. Hargreaves, R. Hashemi, E. V. Karlovets, F. M. Skinner, E. K. Conway, C. Hill, R. V. Kochanov, Y. Tan, P. Wcisło, A.A. Finenko, K. Nelson, P. F. Bernath, M. Birk, V. Boudon, A. Campargue, K. V. Chance, A. Coustenis, B. J. Drouin, J.-M. Flaud, R. R. Gamache, J. T. Hodges, D. Jacquemart, E. J. Mlawer, A. V. Nikitin, V.I. Perevalov, M. Rotger, J. Tennyson, G. C. Toon, H. Tran, V. G. Tyuterev, E. M. Adkins, A. Baker, A. Barbe, E. Canè, A. G. Császár, A. Dudaryonok, O. Egorov, A. J. Fleisher, H. Fleurbaey, A. Foltynowicz, T. Furtenbacher, J. J. Harrison, J.M. Hartmann, V.- M. Horneman, X. Huang, T. Karman, J. Karns, S. Kassi, I. Kleiner, V. Kofman, F. Kwabia-Tchana, N.N. Lavrentieva, T. J. Lee, D. A. Long, A. A. Lukashevskaya, O. M. Lyulin, V. Yu Makhnev, W. Matt, S. T. Massie, M. Melosso, S. N. Mikhailenko, D. Mondelain, H.S.P. Müller, O. V. Naumenko, A. Perrin, O. L. Polyansky, E. Raddaoui, P. L. Raston, Z. D. Reed, M. Rey, C. Richard, R. Tóbiás, I. Sadiek, D. W. Schwenke, E. Starikova, K. Sung, F. Tamassia, S. A. Tashkun, J. Vander Auwera, I.A. Vasilenko, A.A. Vigasin, G.L. Villanueva, B. Vispoel, G. Wagner, A. Yachmenev, S. N. Yurchenko

Chemistry & Biochemistry Faculty Publications

The HITRAN database is a compilation of molecular spectroscopic parameters. It was established in the early 1970s and is used by various computer codes to predict and simulate the transmission and emission of light in gaseous media (with an emphasis on terrestrial and planetary atmospheres). The HITRAN compilation is composed of five major components: the line-by-line spectroscopic parameters required for high-resolution radiative-transfer codes, experimental infrared absorption cross-sections (for molecules where it is not yet feasible for representation in a line-by-line form), collision-induced absorption data, aerosol indices of refraction, and general tables (including partition sums) that apply globally to the data. …

An Electrochemical, Fluidic, Chip-Based Biosensor For Biomarker Detection, Lauren Bell

Theses and Dissertations--Biomedical Engineering

Biosensors and their use in both the research and clinical field for the detection and monitoring of critical biomarkers are prevalent and constantly improving. However, continued research needs to be done to address shortcomings, such as low sensitivity, poor specificity, and poor readiness for integration into research use and patient care. The objective of this research was to create a combined fluidic, chip-based biosensor that could detect different biomarkers with high sensitivity and ease of use. For assessing the developed sensor, three separate biomarkers were tested: glucose, cholesterol, and oxygen. Both the glucose biosensor and cholesterol biosensor were combined with …

Characterizing The Internal Porous Structure Of Equine Proximal Sesamoid Bones Subjected To Race Training Using Fast Fourier Transforms, Joseph Erik Davis

Theses and Dissertations--Biomedical Engineering

The equine racing industry is one of the main proponents in Kentucky’s economic infrastructure. In this industry there has come a need to investigate the nature of the proximal sesamoid bone (PSB). Breakdowns involving the PSBs are the leading cause in racehorse deaths in the industry, with still little known about what causes this bone to fracture. This study seeks to shed insight by investigating the internal structure of the PSB. Using microCT scanning, the internal porous structure was captured. From there, noticeable differences in the pores were noticed and quantified using fast Fourier transform (FFT) analysis.

The dominant peak …

Use Of Image Processing Techniques And Machine Learning For Better Understanding Of T Gondii Biology, Amer Asiri

Theses and Dissertations--Biomedical Engineering

Almost one in every three people worldwide is infected with Toxoplasma gondii (T. gondii). The biology and growth of the parasite’s bradyzoite form in host tissue cysts are not well understood. T. gondii’s metabolic state influences the morphology of its single mitochondrion, which can be visualized using fluorescence microscopy with specific dyes. Hence, fluorescence microscopy images of cysts purified from infected mouse brains carry biological information about bradyzoites, the poorly understood form of the parasite within them. With the help of fluorescence microscopy techniques, previous studies extracted images of the mitochondrion, nucleus, and the inner membrane complex (IMC) …

Numerical And Scaling Study On Application Of Inkjet Technology To Automotive Coating, Masoud Arabghahestani Dr.

Theses and Dissertations--Mechanical and Aerospace Engineering

A thorough literature review identified lack of precision control over quality of droplets generated by the currently available industrial sprayers and a growing need for higher quality droplets in the coating industry. Particularly, lack of knowledge and understanding in continuous inkjets (CIJ) and drop-on-demand (DOD) technologies is identified as significant. Motivated by these needs, this dissertation is dedicated to computational fluid dynamics (CFD) and scaling studies to improve existing inkjet technologies and develop new designs of efficient coating with single and/or multiple piezoelectric sensors to produce on-demand droplets. This dissertation study aims at developing a new DOD type coating technology, …

Atmospheric Air Plasma Streamers Deliver Nanosecond Pulses For Focused Electroporation, Shu Xiao, Carol Zhou, Eric Appia, Shirshak Dhali

Bioelectrics Publications

Background: For electrotherapies that involve electrodes and high-intensity electric fields, such as in tissue ablation, we report a method of pulse delivery that can focus the electric field away from the electrodes, as demonstrated in vitro.

Materials and Methods: To electroporate cells in a monolayer seeded in a 35 mm culture dish, two atmospheric-pressure plasma channels generated by two thin, copper foil electrodes above the surface of the solution provided the current and established the electric field.

Results: Depending on the pulse duration, the plasma channels were observed as corona (100 ns), streamer (300 ns), and mixture of streamer …

Optimization Of Electrically Driven Fluid Flow Through Tissue Hydrogels, Joseph C. Walker

Electronic Theses and Dissertations

No abstract provided.

Reporter Chondrocyte Optimization Of Bioinks For Zonal 3d Bioprinting Of Cartilage, Kari Martyniak

Electronic Theses and Dissertations, 2020-2023

Osteoarthritis (OA) is the most common form of arthritis, often thought of as a disease of the elderly, but post traumatic OA predominantly impacts younger individuals. Articular cartilage is the tissue that coats the end of your bones in synovial joints. Since cartilage has limited healing capacity, defects, or injuries to it progressively erodes down to the subchondral bone. Unfortunately, current treatment options all have limitations, particularly for younger patients. Cartilage has a specific zonal architecture that is distinguished by the different cell morphologies and arrangements, biochemical composition, and mechanical properties. 3D bioprinting is a tissue engineering technique that involves …

Challenges Of Constructing Entrainment Map For Arbitrary Circadian Models, Yuxuan (Nelson) Wu

Honors Theses

The entrainment map, developed by Dr.Diekman and Dr.Bose, is claimed to be a 1-dimensional map that produces a better prediction for phase-locking than methods than the phase response curve for circadian models. In his paper, he constructs the entrainment map for the two-dimensional circadian model, the Novak-Tyson model, and the other two higher-dimensional circadian models. For this thesis, we concentrate on exploring if it is viable to construct the entrainment map for other circadian models that are not included in his paper: the Becker-Weimann model and the Relogio model. In addition, we discuss the challenges of constructing the entrainment map …

Alkaline Plasma-Activated Water (Paw) As An Innovative Therapeutic Avenue For Cancer Treatment, Bolun Pang, Zhijie Liu, Sitao Wang, Yuting Gao, Miao Qi, Dehui Xu, Renwu Zhou, Dingxin Liu, Michael G. Kong

Bioelectrics Publications

Plasma-activated water (PAW) is considered to be an effective anticancer agent due to the diverse aqueous reactive oxygen and nitrogen species (RONS: ROS and RNS), but the drawback of low dose and short duration of RONS in acidified PAW limits their clinical application. Herein, this Letter presents an innovative therapeutic avenue for cancer treatment with highly-effective alkaline PAW prepared by air surface plasma. This anticancer alkaline formulation is comprised of a rich mixture of highly chemical RONS and exhibited a prolonged half-life compared to acidified PAW. The H₂O₂, NO₂-, and ONOO^-/O_{2 …}

Framework For The Evaluation Of Perturbations In The Systems Biology Landscape And Inter-Sample Similarity From Transcriptomic Datasets — A Digital Twin Perspective, Mariah Marie Hoffman

Dissertations and Theses

One approach to interrogating the complexities of human systems in their well-regulated and dysregulated states is through the use of digital twins. Digital twins are virtual representations of physical systems that are descriptive of an individual's state of health, an object fundamentally related to precision medicine. A key element for building a functional digital twin type for a disease or predicting the therapeutic efficacy of a potential treatment is harmonized, machine-parsable domain knowledge. Hypothesis-driven investigations are the gold standard for representing subsystems, but their results encompass a limited knowledge of the full biosystem. Multi-omics data is one rich source of …

Analysis Of The Epidemiology Of Playground-Related Injuries In Relation To Existing Playground Safety Standards: How Should We Address Safe Play?, Drew Filchner

Honors Theses

Play is widely acknowledged to provide children with various physical, emotional, social, and intellectual benefits. However, playgrounds are reported to be the leading location of unintentional recreation injuries to children aged 1-10. Over 185,000 children annually are being treated in emergency departments for playground injuries. These play-related injuries have remained stagnant according to epidemiology studies of the past three decades. Past epidemiological studies for playground injuries have been based on emergency department data, resulting in an underrepresentation of the magnitude of injuries occurring. This study utilizes the unique setting of an entire school district, providing a more comprehensive understanding of …

Tumor-Targeted Ph-Sensitive Manganese Oxide Nanoparticle For Enhanced Detection Of Breast Cancer Using Mri, Celia Martinez De La Torre

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the United States, approximately 287,850 new cases of invasive breast cancer will be diagnosed, and over 43,000 women will die because of it in 2022. Currently, mammography is the gold standard for breast cancer surveillance. Although this detection technique is relatively cheap and fast, it misses about 1 in 5 breast cancers in younger women with dense breast tissue, causing a delay in treatment. Compared to mammography, magnetic resonance imaging (MRI) has superior soft-tissue contrast that elucidates structural differences within soft tissue to detect more breast cancers. However, MRI still misses

NEMO particles were produced by synthesizing manganese oxide …

Radial Artery Model For Catheterization Procedures, Kyla Beville, Mitchell Gagnon, Benjamin Stalls, Maya Ariza

Williams Honors College, Honors Research Projects

The purpose of this project was to create a radial artery model that is usable under ultrasound that would reduce complications during catheterization procedures. Each year, over one million of these procedures are done and complications can affect the outcomes of the patient. Using ultrasound during these procedures is an upcoming way to reduce these complications and improve the outcome that a patient will have.

Hands Free Fitted Shoe, Maddison Schutt, Nicole Rizkala, Elizabeth Scheatzle, Alexander Hershey, Austin Fowkes

Williams Honors College, Honors Research Projects

The ability to don and doff fitted shoes can be a challenge for many people including those diagnosed with diabetes, obesity, limb loss, pregnancy, Parkinson’s disease, arthritis, surgical complications, and aging. Though these actions may seem trivial, the ability to independently put on shoes is necessary because it can significantly impact one’s capacity to complete everyday tasks, hold relationships, and maintain mental health. Current competitors in the market are excessively expensive, not completely hands-free, or not fully enclosed shoes, so a new design for an accessible and affordable hands-free fitted shoe could be of great benefit to the general population. …

Erk3 And Dgkζ Interact To Modulate Cell Motility In Lung Cancer Cells, Amanda Myers

Browse all Theses and Dissertations

Extracellular-regulated kinase 3 (ERK3) is overexpressed in a variety of cancers, including lung cancer. This atypical mitogen-activated protein kinase (MAPK) has a unique structure which includes a C34 domain and C-terminus and about which relatively little is known. ERK3 has been demonstrated to promote cell migration and metastasis in multiple cancer types. A yeast two-hybrid assay using ERK3 as bait indicated Diacylglycerol kinase ζ (DGKζ) is a binding partner. DGKζ is an isoform in the DGK family, all the members of which phosphorylate the lipid diacylglycerol (DAG) to generate phosphatidic acid (PA). DGKζ was shown to promote migration in mouse …