Biological Engineering Commons^™

Ultrasonic Concentration Of Microorganisms, Samuel J. Mullins

Theses and Dissertations--Biosystems and Agricultural Engineering

Concentration of microorganisms from a sample volume would increase the limits of detection of samples used for rapid-detection methods. Rapid detection methods are is advantageous for the food industry to rapidly test for bacteria in order release products on a timely basis. Ultrasonic concentration was considered a promising method for manipulation of microorganisms. An ultrasonic chamber consisting of parallel piezoceramic discs with a reticulated polyurethane foam mesh was used to concentrate Saccharomyces cerevisiae yeast and Escherichia coli bacteria. The concentration of yeast was seen to increase by 200% (from 8.0 x 10⁴ cells mL^-1 to 2.4 x 10 …

Complete Genome Sequence Of Clostridium Clariflavum Dsm 19732, Javier A. Izquierdo, Lynne Goodwin, Karen W. Davenport, Hazuki Teshima

Dartmouth Scholarship

Clostridium clariflavum is a Cluster III Clostridium within the family Clostridiaceae isolated from thermophilic anaerobic sludge (Shiratori et al, 2009). This species is of interest because of its similarity to the model cellulolytic organism Clostridium thermocellum and for the ability of environmental isolates to break down cellulose and hemicellulose. Here we describe features of the 4,897,678 bp long genome and its annotation, consisting of 4,131 protein-coding and 98 RNA genes, for the type strain DSM 19732.

Evaluation Of The Impact Of Surface Residue Cover On Single And Dual Crop Coefficient For Estimating Soybean Actual Evapotranspiration, Lameck O. Odhiambo, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Single and dual crop coefficient methods are used in conjunction with grass reference evapotranspiration (ET_o) to estimate actual crop evapotranspiration (ET_c). However, the impact of soil surface residue cover on the accuracy of ET_c estimated with these methods is not well understood. The objective of this study is to evaluate and compare the accuracy of the FAO-56 single crop coefficient (single-K_c) and dual crop coefficient (dual-K_c) methods for estimating soybean [Glycine max (L.) Merr.] ET_c in a partially residue covered field. The study was conducted …

Carbon, Water, And Heat Flux Responses To Experimental Burning And Drought In A Tallgrass Prairie, Marc L. Fischer, M S. Torn, David P. Billesbach, Geoffrey Doyle, Brian Northup, Sebastien C. Biraud

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Drought and fire are common disturbances to grassland ecosystems. We report two years of eddy covariance ecosystem–atmosphere fluxes and biometric variables measured in nearby burned and unburned pastures in the US Southern Great Plains. Over the course of the experiment, annual precipitation (∼600 mm yr⁻¹) was lower than the long term mean (∼860 mm yr⁻¹). Soil moisture decreased from productive conditions in March 2005 dry, unproductive conditions during the growing season starting in March 2006. Just prior to the burn in early March 2005, burned and unburned pastures contained 520 ± 60 and 360 ± 40 …

Subsurface Phosphorus Transport And Scale Dependent Phosphorus Leaching In Alluvial Floodplains, Derek Michael Heeren

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Increased nutrient loads have resulted in several adverse impacts on surface water quality, including excessive algal growth, fish kills, and drinking water taste and odor issues across the United States and especially in the Ozark ecoregion of northeastern Oklahoma and northwestern Arkansas. Nitrogen is a concern, but phosphorus (P) is generally considered the limiting nutrient in most surface water systems. Scientists and engineers need to identify critical nutrient source areas and transport mechanisms within a catchment in order to cost effectively protect and enhance drinking water systems, recreation activities, and aquatic ecosystems. While surface runoff is considered to be the …

Computational Analysis Of Hybrid Norwood Circulation With Distal Aortic Arch Obstruction And Reverse Blalock-Taussig Shunt, Andres Ceballos, I. Ricardo Argueta-Morales, Eduardo Divo, Ruben Osorio, Christopher A. Caldarone, Alain J. Kassab, William M. Decampli

Mechanical Engineering - Daytona Beach

BACKGROUND: The hemodynamics characteristics of the hybrid Norwood (HN) procedure differ from those of the conventional Norwood and are not fully understood. We present a multi-scale model of HN circulation to understand local hemodynamics and effects of aortic arch stenosis and a reverse Blalock-Taussig shunt (RBTS) on coronary and carotid perfusion. METHODS: Four 3-dimensional models of four HN anatomic variants were developed, with and without 90% distal preductal arch stenosis and with and without a 4-mm RBTS. A lumped parameter model of the circulation was coupled to a local 3-dimensional computational fluid dynamics model. Outputs from the lumped parameter model …

An Investigation Of Reflective Mulches For Use Over Capillary Mat Systems For Winter-Time Greenhouse Strawberry Production, George E. Meyer, Ellen T. Paparozzi, Elizabeth Walter-Shea, Erin E. Blankenship, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

Photosynthethically active radiation (PAR) is a principle environmental variable used by horticultural specialists, agronomists and ecosystem modelers to characterize the quantity and quality of light conducive to plant growth and development. Spatial distribution of PAR in a greenhouse can be quite variable and diffuse throughout the day time photoperiod, especially at low sun angles in northern regions of the United States. Four colors of reflective plastic mulches (white, red, olive, and black) were evaluated for winter-time strawberry (Fragaria × ananassa Duch.) production based on their reflectance and transmittance properties in a double-polyethylene, plastic-glazed Quonset greenhouse inNebraska. The spectral properties …

Enhanced Microbial Utilization Of Recalcitrant Cellulose By An Ex Vivo Cellulosome-Microbe Complex, Chun You, Xiao-Zhou Zhang, Noppadon Sathitsuksanoh, Lee R. Lynd

Dartmouth Scholarship

A cellulosome-microbe complex was assembled ex vivo on the surface of Bacillus subtilis displaying a miniscaffoldin that can bind with three dockerin-containing cellulase components: the endoglucanase Cel5, the processive endoglucanase Cel9, and the cellobiohydrolase Cel48. The hydrolysis performances of the synthetic cellulosome bound to living cells, the synthetic cellulosome, a noncomplexed cellulase mixture with the same catalytic components, and a commercial fungal enzyme mixture were investigated on low-accessibility recalcitrant Avicel and high accessibility regenerated amorphous cellulose (RAC). The cellbound cellulosome exhibited 4.5- and 2.3-fold-higher hydrolysis ability than cell-free cellulosome on Avicel and RAC, respectively. The cellulosome-microbe synergy was not completely …

Effect Of Extracellular Matrix (Ecm) Protein Micropatterns On The Behavior Of Human Neuroblastoma Cells, Ishwari Poudel

Department of Engineering Mechanics: Dissertations, Theses, and Student Research

Recent advances in patterning techniques and emerging surface microtechnologies have allowed cell micropatterning to control spatial location of the cells on a surface as well as cell shape, attachment area, and number of contacting neighbor cells. These parameters play important roles in cell cellular behaviors. Cell micropatterning has thus become one of the most important strategies for biomedical applications, such as, tissue engineering, diagnostic immunoassays, lab-on-chip devices, bio-sensing, etc., and cell biology studies as well. For neuronal cells, there have been attempts to distribute neuronal cells on specific patterns to control cell-to-cell interaction. However, there have been very limited understanding …

A Novel Telecommunications-Based Approach To Mathematical Modeling Of Hiv Infection, Aaron T. Sharp

Department of Computer Electronics and Engineering: Dissertations, Theses, and Student Research

It is well known that biological systems utilize communication in some form, one prolific example of this is the propagation of HIV (Human Immunodeficiency Virus) in the human body. By modeling HIV infection as a communication system, we hope to gain a unique insight into HIV and biological communication systems in general. Such a model would provide researchers a platform for experimenting and simulating various biological communication systems. We have previously developed a layered communication protocol for interpreting biological communication systems using telecommunications paradigms and will apply said model to HIV proliferation. We will also demonstrate the effectiveness of the …

Studies On Power Hop Using A Nonlinear Model Of An Agricultural Tractor, Adam B. Flaugh

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Power hop is an unstable dynamic phenomenon that is observed on agricultural tractors operating at field working speeds under typical draft loads. This dynamic instability can be identified as a bounce motion preceded by an increasing oscillation in either the pitch or bounce modes. A definite and comprehensive correction or prevention strategy for the control of power hop does not exist. In this work a linear and a nonlinear model of an agricultural tractor were developed. The method of multiple time scales has been employed in the analysis of the nonlinear model. Numerical results have been obtained using Mathematica to …

The Economics And Logistics Of The Dual Harvest Of Grain And Biomass In A Single-Pass, Matthew T. Wold

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Significant interest has developed in using cellulosic resources, especially crop residues, to create biofuels. Collecting these residues in a single-pass of the harvester across the field has the potential to be a low cost option. Two models have been developed; the first characterizes the in-field logistics of single-pass crop residue collection, the second the economics. These models allow the user to easily examine a wide variety of both grain-only and single-pass residue collection harvest cases. A variety of possible residue collection cases have been examined, and their effects both on harvester field capacity and harvest cost compared to grain-only harvest …

Design And Fabrication Of Nanofluidic Systems For Biomolecule Characterizations, Orain Ansel Hibbert

Graduate Theses and Dissertations

Nanofluidic channel systems were designed and fabricated by combining MEMS microfabrication with AFM nanolithography. In the fabrication process flow, photolithography was first utilized to pattern microfluidic channels and reservoirs on a 4" Pyrex substrate. Subsequently, atomic force microscopy (AFM) based nanolithography was used to mechanically fabricate nanochannels to connect the microreservoirs which formed the inlet and outlet of the nanofluidic system. A Tap190 Diamond-Like Carbon (DLC) AFM tip with a force constant of 48 N/m and a radius of less than 15 nm was used as the nanolithography tool. The resultant nanochannel ranges from 20 to 80 µm in length …

Designing Predictive Mathematical Models For The Metabolic Pathways Associated With Polyhydroxybutyrate Synthesis In Escherichia Coli, Angela Dixon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Plastics are a versatile and widely used material. However, traditional plastics are derived from petrochemicals and are not biodegradable. Polymers synthesized from microorganisms that have similar properties to plastic are potential biodegradable replacements. The objective of this project is to use mathematical modeling as a tool to engineer a strain of bacteria optimized for the production of bio-plastics.

Production costs can be reduced by using a bacterial strain specifically optimized for bio-plastic production. By reducing production costs, bio-plastics will be able to commercially compete with traditional plastics. Society will benefit as bio-plastics replace traditional plastics. Fossil fuels will not be …

Climate Change Policy Could Make Keystone Xl Obsolete, Adam Liska

Adam Liska Papers

Clearly the Keystone XL pipeline threatens both surface and ground water that sustains the agricultural economy of Nebraska, but the projected profitability of tar sands oil and the pipeline operation are dependent on the future economics of climate change. Three recent scientific studies estimate the total carbon intensity of tar sands-based gasoline from Alberta (from both production and burning of the fuel) at an average of roughly 22 percent higher than U.S. gasoline in 2005, with the range of average carbon intensities for tar sands-gasoline at 16 to 27 percent higher than most other sources.

Comparison Of Four Chip-Seq Analytical Algorithms Using Rice Endosperm H3k27 Trimethylation Profiling Data., Brandon M. Malone, Feng Tan, Susan M. Bridges, Zhaohua Peng

BCoE Publications

Chromatin immunoprecipitation coupled with high throughput DNA Sequencing (ChIP-Seq) has emerged as a powerful tool for genome wide profiling of the binding sites of proteins associated with DNA such as histones and transcription factors. However, no peak calling program has gained consensus acceptance by the scientific community as the preferred tool for ChIP-Seq data analysis. Analyzing the large data sets generated by ChIP-Seq studies remains highly challenging for most molecular biology laboratories.Here we profile H3K27me3 enrichment sites in rice young endosperm using the ChIP-Seq approach and analyze the data using four peak calling algorithms (FindPeaks, PeakSeq, USeq, and MACS). Comparison …

Protective Effects Of Milk Phospholipids Against Uv Photodamage In Human Skin Equivalents, Zyra Achay

Master's Theses

The ultraviolet (UV) spectrum has been known to cause damage to skin in varying degrees. UVB radiation (290-320 nm) in particular, has been proven to be highly mutagenic and carcinogenic in many animal experiments compared to either UVA or UVC. The alarming rate of increase in skin cancer incidence has prompted many investigators to pursue other alternatives to sunscreens including changes in lifestyle habits and dietary consumption in order to boost our efforts in tackling this widespread disease. Previous studies employing confocal reflectance, MTT assay and histology suggest that milk phospholipids may possess protective properties against UVB-mediated damage but the …

Mutant Alcohol Dehydrogenase Leads To Improved Ethanol Tolerance In Clostridium Thermocellum, Steven D. Brown, Adam M. Guss, Tatiana V. Karpinets, Jerry M. Parks

Dartmouth Scholarship

Clostridium thermocellum is a thermophilic, obligately anaerobic, Gram-positive bacterium that is a candidate microorganism for converting cellulosic biomass into ethanol through consolidated bioprocessing. Ethanol intolerance is an important metric in terms of process economics, and tolerance has often been described as a complex and likely multigenic trait for which complex gene interactions come into play. Here, we resequence the genome of an ethanol-tolerant mutant, show that the tolerant phenotype is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE), hypothesize based on structural analysis that cofactor specificity may be affected, and confirm this hypothesis using enzyme assays. …

Methods Of Using Black Bear Parathyroid Hormone, Seth W. Donahue

Michigan Tech Patents

Black bear parathyroid hormone (PTH) and functional fragments thereof are provided. Also provided are methods of using black bear PTH and functional fragments for increasing cAMP in a bone-forming cell; reducing apoptosis in a boneforming cell; decreasing the ratio of expression levels of Bax protein to Bcl-2 protein in a bone-forming cell; increasing the expression level of one or more of a bone matrix protein, a transcriptional activator, or a transcriptional regulator in a bone-forming cell; enhancing bone mineral density, increasing bone mass, decreasing bone loss, or reducing the incidence of bone fractures, or any combination thereof, in a subject; …

Forward Osmosis Bag: Ground Testing The Prospect Of Using Alternative Water Sources For Drinking Water, Michaela Marie Brant, Ariella Yendler, Mona Hammoudeh, Michael T. Flynn

STAR Program Research Presentations

On the STS-135 Space Shuttle mission, to be launched July 8, 2011, a forward osmosis bag (FOB) study will be conducted. At NASA Ames this summer, the ground truth testing is being conducted for results comparisons. The FOB technology is derived from a commercial product, the X-Pack water filter. Forward osmosis operates by utilizing an established concentration gradient across a semi-permeable membrane to move water molecules from one side of the membrane to the other. This concept is exploited to harvest drinking water from grey water sources such as urine, sea water, or vehicle water. In this experiment, potassium chloride …