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Molecular Genetic Studies Of Horses, Especially With Reference To Aggrecan And Dwarfism, John Edmund Eberth 2023 University of Kentucky

Molecular Genetic Studies Of Horses, Especially With Reference To Aggrecan And Dwarfism, John Edmund Eberth

Theses and Dissertations--Veterinary Science

This work consists of studies on dwarfism in Miniature horses and a study on breakdowns in Thoroughbreds in relation to gene aggrecan. A correction of the description and designation of D3 dwarf variant in aggrecan (ACAN) from the master’s thesis Chondrodysplasia-like dwarfism in the Miniature horse (2013). Commercial sequencing showed previous sequencing reads presented an artifact and not a single base deletion. Analysis showed a single base missense mutation in exon 8 identified as D3* was the actual cause. Multiple alleles of ACAN associated with chondrodysplastic dwarfism in Miniature horses by Eberth et al. (2018), corrected the …


Evaluating Efficacy Of Anti-Batrachochytrium Dendrobatidis Probiotic Treatment On Pacific Chorus Frogs (Pseudacris Regilla) At Current And Modeled Climate Change Temperatures, Autumn N. Holley 2023 Eastern Washington University

Evaluating Efficacy Of Anti-Batrachochytrium Dendrobatidis Probiotic Treatment On Pacific Chorus Frogs (Pseudacris Regilla) At Current And Modeled Climate Change Temperatures, Autumn N. Holley

EWU Masters Thesis Collection

Amphibian populations are declining due to a variety of threats, including the chytrid fungal pathogen, Batrachochytrium dendrobatidis (Bd), the causative agent of the disease chytridiomycosis. Climate change and other stressors can have complicated interactions with amphibian disease. Some amphibian populations are less susceptible to chytridiomycosis due to factors such as symbiotic skin microbes that may inhibit Bd through secondary metabolites. There have been several attempts to develop probiotics from these symbiotic, antifungal bacteria to provide protection against Bd infection, but these studies have had mixed success. Our study evaluated anti-Bd bacteria isolated from the skin of Pacific chorus frogs (Pseudacris …


Investigation Of Small Mammal Species Richness, Abundance, And Genetic Population Structure On And Around The Eastern Washington University Prairie Restoration Site, Sarah Deshazer 2023 Eastern Washington University

Investigation Of Small Mammal Species Richness, Abundance, And Genetic Population Structure On And Around The Eastern Washington University Prairie Restoration Site, Sarah Deshazer

EWU Masters Thesis Collection

Small mammals are an ecologically important component of every landscape on Earth. They are a food source for higher trophic level animals, disperse plant seed and mycorrhizal fungi spore, engineer the landscape through burrowing and foraging activities, and alter plant community composition through selective predation of seed and grain. Studies have shown that small mammals may help facilitate the transition between successive stages in prairie restoration. Eastern Washington University has dedicated 120 acres of campus land to restoration of native prairie habitat. Small mammals can play both a positive and a negative role in restoration, therefore it is important to …


Improving Indoor Arenas For The Equine Industry, Staci McGill 2023 University of Kentucky

Improving Indoor Arenas For The Equine Industry, Staci Mcgill

Theses and Dissertations--Biosystems and Agricultural Engineering

Equine indoor arenas are a unique infrastructure investment found at equine farms and facilities. They are semi-indoor structures for exercising horses, exhibiting skills during competitive events, and other equine related activities. These spaces do not always include mechanical ventilation or stirring fans and occupancy by horses and humans can be sporadic and inconsistent, which creates a challenging space for understanding and predicting airflow. Typically, indoor arenas have a sand-based footing over which the horse travels. The impact of the hooves can cause dust to become a concern within the facilities. Environmental concerns within these facilities (temperature, respirable dust, moisture, and …


Cluster Homolog Of Immunoglobulin-Like Receptor Genes In Chicken Immune Responses, Brandi A. Sparling 2023 West Virginia University, Western University of Health Sciences

Cluster Homolog Of Immunoglobulin-Like Receptor Genes In Chicken Immune Responses, Brandi A. Sparling

Graduate Theses, Dissertations, and Problem Reports

This dissertation explores the identity and role of immunoglobulin-like (Ig-like) receptors in chickens, with focus on their implications in disease and disease progression. These receptors, wisely expressed across immune cells, interact with the major histocompatibility complex (MHC) class I molecules to modulate immune responses in mammals. Due to the insufficient representation of chicken Ig-like receptors in online databases, this study systematically annotates the chicken Cluster Homolog of Immunoglobulin-like Receptors (CHIR) genes using advanced bioinformatic techniques, aligning with the release of the 7th edition of the chicken genome assembly that comprises builds for a broiler and layer chicken. The analysis identifies …


Amphisbaenian Head Movement And Burrowing Forces In Damp Granular Media, Jacob Newell 2023 The University of Akron

Amphisbaenian Head Movement And Burrowing Forces In Damp Granular Media, Jacob Newell

Williams Honors College, Honors Research Projects

Damp granular media is a difficult environment to study because it is both practically complex and it lacks equations which fully describe its behavior. In this study, an oscillatory lateral head movement and its effects while penetrating damp granular media were tested using a robophysical model. This experimental research was inspired by the burrowing behavior of the clade Amphisbaenia, a group of usually limbless squamates that employ a variety of different burrowing behaviors, but it can apply to a wide range of burrowers. This research could help with both human burrowing technologies and the further investigation of animal behaviors.


Effects Of Duckweed On Mosquito Oviposition And Larval Survivorship, Alyssa Gushka 2023 Arcadia University

Effects Of Duckweed On Mosquito Oviposition And Larval Survivorship, Alyssa Gushka

Capstone Showcase

Invasive species are those non-natives to habitat and that negatively impact the environment or human health problems. Invasive mosquitoes threaten the environment and humans, as they transmit 17% of infectious diseases including malaria, West Nile virus and yellow fever. Aedes japonicas and Aedes albopictus are invasive mosquitoes that often deposit eggs in artificial containers. Chemical pesticides, such as Bacillus thuringiensis israelensis (BTI), utilizes endospores to release toxins into the larvae. However, BTI creates negative consequences for the environment and other species. Many plants are being examined to see if they can act as natural pesticides. Duckweeds are native to North …


Sustainability Practices In Aquaculture: Using Algae Turf Scrubber Biomass To Raise Black Soldier Flies As An Alternative Feed In Blue Tilapia, Oreochromis Aureus, Culture, Michelle C. Lowery 2023 Georgia Southern University

Sustainability Practices In Aquaculture: Using Algae Turf Scrubber Biomass To Raise Black Soldier Flies As An Alternative Feed In Blue Tilapia, Oreochromis Aureus, Culture, Michelle C. Lowery

Electronic Theses and Dissertations

Since the 1980s, non-algal aquaculture has grown to encompass 49% of all seafood production in response to a growing human population and increased seafood demand (FAO, 2022). Hurdles exist to aquaculture sustainability, including dependence on wild sourced fishmeal (FM) and the impacts wastewater discharge. It takes 4-5 tons of wild forage fish to produce one ton of dry FM (Miles and Chapman, 2006) and as aquaculture is primarily conducted in earthen ponds and public open water bodies (FAO, 2022), finfish culture can have a high impact on the surrounding environment by discharging excess nutrients. This study used algae turf scrubber …


Effects Of Increasing Levels Of Soybean Meal In Nursery Diets On Growth Performance And Fecal Characteristics Of 22- To 60-Lb Pigs, Jamil E. G. Faccin, Robert D. Goodband, Mike D. Tokach, Joel M. DeRouchey, Jordan T. Gebhardt, Jason C. Woodworth 2023 Kansas State University, Manhattan

Effects Of Increasing Levels Of Soybean Meal In Nursery Diets On Growth Performance And Fecal Characteristics Of 22- To 60-Lb Pigs, Jamil E. G. Faccin, Robert D. Goodband, Mike D. Tokach, Joel M. Derouchey, Jordan T. Gebhardt, Jason C. Woodworth

Kansas Agricultural Experiment Station Research Reports

Two experiments were conducted to determine the effects of increasing soybean meal (SBM) on late nursery pig performance. In Exp. 1, a total of 266 pigs (241 × 600 DNA; initially 22.2 ± 0.37 lb) were used in a 21-d trial with 14 replicate pens per treatment and 4 to 5 pigs per pen. Pens of pigs were randomly assigned to 1 of 4 dietary treatments which were corn-based with SBM levels of 25.0, 28.9, 32.5, or 36.2%. In Exp. 2, a total of 340 pigs (241 × 600 DNA; initially 29.8 ± 0.40 lb) were used in a 21-d …


Evaluation Of Precision Feeding Sid Lysine To Lactating Sows On Sow And Litter Performance, Nitrogen Level, And Feed Cost, Mikayla S. Spinler, Jordan T. Gebhardt, Joel M. DeRouchey, Mike D. Tokach, Robert D. Goodband, Hyatt L. Frobose, Jason C. Woodworth 2023 Kansas State University, Manhattan

Evaluation Of Precision Feeding Sid Lysine To Lactating Sows On Sow And Litter Performance, Nitrogen Level, And Feed Cost, Mikayla S. Spinler, Jordan T. Gebhardt, Joel M. Derouchey, Mike D. Tokach, Robert D. Goodband, Hyatt L. Frobose, Jason C. Woodworth

Kansas Agricultural Experiment Station Research Reports

A total of 95 mixed parity sows (DNA 241) and litters (DNA 241 × 600) were used across four batch farrowing groups to evaluate the effects of precision feeding Lys during lactation. Sows were blocked by parity and allotted to 1 of 3 treatments on day 2 (the day after farrowing) of lactation. Dietary treatments were formed by using 2 diets: a low Lys diet (0.25% SID Lys) and a high Lys diet (1.10% SID Lys). Treatments included a control, NRC (2012), or INRA (2009) treatment curve. Sows on the NRC or INRA treatment curves received a blend of the …


Effect Of Sulfate Or Hydroxychloride Forms Of Zinc, Manganese, And Copper On Growth Performance, Weight Variation, Carcass Characteristics, And Economics Of Grow-Finish Pigs, Hilario M. Cordoba, Jason C. Woodworth, Robert D. Goodband, Joel M. DeRouchey, Mike D. Tokach, Jordan T. Gebhardt, Chris P.A. van de Ligt 2023 Kansas State University, Manhattan

Effect Of Sulfate Or Hydroxychloride Forms Of Zinc, Manganese, And Copper On Growth Performance, Weight Variation, Carcass Characteristics, And Economics Of Grow-Finish Pigs, Hilario M. Cordoba, Jason C. Woodworth, Robert D. Goodband, Joel M. Derouchey, Mike D. Tokach, Jordan T. Gebhardt, Chris P.A. Van De Ligt

Kansas Agricultural Experiment Station Research Reports

A total of 1,026 grow-finish pigs (337 × 1050 PIC; initially 57.2 ± 0.73 lb) were used in a 124-d trial to compare sulfate and hydroxychloride forms of Zn, Mn, and Cu on growth performance, carcass characteristics, weight variation, and economics of grow-finish pigs. Pigs were housed in mixed gender pens with 27 pigs per pen and 19 pens per treatment. The treatments were structured as a completely randomized design and consisted of a control diet containing 150, 16, and 110 ppm of Cu, Mn, and Zn, respectively, from sulfate sources or the same inclusion provided by hydroxychloride sources. Experimental …


Effects Of Standardized Ileal Digestible Threonine To Lysine Ratio On Growth Performance Of Pic Line 337 × 1050 Pigs, Rafe Q. Royall, Mike D. Tokach, Jason C. Woodworth, Joel M. DeRouchey, Robert D. Goodband, Jordan T. Gebhardt, Carine M. Vier, Matthew Spindler, Uislei Orlando, Luis Zaragoza, Ning Lu, Wayne Cast, Danielle F. Wilson-Wells, Julia P. Holen, Alyssa M. Betlach 2023 Kansas State University, Manhattan

Effects Of Standardized Ileal Digestible Threonine To Lysine Ratio On Growth Performance Of Pic Line 337 × 1050 Pigs, Rafe Q. Royall, Mike D. Tokach, Jason C. Woodworth, Joel M. Derouchey, Robert D. Goodband, Jordan T. Gebhardt, Carine M. Vier, Matthew Spindler, Uislei Orlando, Luis Zaragoza, Ning Lu, Wayne Cast, Danielle F. Wilson-Wells, Julia P. Holen, Alyssa M. Betlach

Kansas Agricultural Experiment Station Research Reports

The objective of this research was to evaluate the impact of varying SID Thr:Lys ratios on growth performance, removals, and mortality rates of late-nursery, grower, and finishing PIC 337 × 1050 pigs. In each experiment, pens of pigs were blocked by BW and randomly assigned to 1 of 5 dietary treatments in a randomized complete block design with 19 to 27 pigs per pen and 8, 7, and 7 replications per treatment in Exp. 1, 2, and 3, respectively. In Exp. 1, 987 pigs (initially 26.0 ± 0.70 lb) were used from 26 to 54 lb. In Exp. 2, 875 …


The Effect Of Live Yeast Probiotics In Lactation Diets With And Without A Yeast Prebiotic In Nursery Diets On Lifetime Growth Performance, Antibody Titers, And Carcass Characteristics, Abigail K. Jenkins, Joel M. DeRouchey, Jordan T. Gebhardt, Jason C. Woodworth, Mike D. Tokach, Robert D. Goodband, Joseph A. Loughmiller, Brian T. Kremer 2023 Kansas State University, Manhattan

The Effect Of Live Yeast Probiotics In Lactation Diets With And Without A Yeast Prebiotic In Nursery Diets On Lifetime Growth Performance, Antibody Titers, And Carcass Characteristics, Abigail K. Jenkins, Joel M. Derouchey, Jordan T. Gebhardt, Jason C. Woodworth, Mike D. Tokach, Robert D. Goodband, Joseph A. Loughmiller, Brian T. Kremer

Kansas Agricultural Experiment Station Research Reports

A total of 28 mixed parity sows (Line 241 DNA) and their offspring were used in a farrow-to-finish study to evaluate the effect of live yeast supplementation during lactation with or without yeast extract supplementation during the nursery period on sow and litter performance and lifetime growth performance, serum antibody titers, and carcass characteristics. Sows were blocked by parity and BW on d 110 of gestation and allotted to 1 of 2 dietary treatments which consisted of a standard corn-soybean meal lactation diet with or without yeast-based probiotics (0.10% Actisaf Sc 47 HR+; Phileo by Lesaffre, Milwaukee, WI). Following weaning, …


Summary Of Methodology Used In Enterotoxigenic Escherichia Coli (Etec) Challenge Experiments In Weanling Pigs And Quantitative Assessment Of Observed Variability, Payton L. Dahmer, Joel M. DeRouchey, Jordan T. Gebhardt, Chad B. Paulk, Cassandra K. Jones 2023 Kansas State University, Manhattan

Summary Of Methodology Used In Enterotoxigenic Escherichia Coli (Etec) Challenge Experiments In Weanling Pigs And Quantitative Assessment Of Observed Variability, Payton L. Dahmer, Joel M. Derouchey, Jordan T. Gebhardt, Chad B. Paulk, Cassandra K. Jones

Kansas Agricultural Experiment Station Research Reports

Post-weaning diarrhea in pigs can be caused by the F4 or F18 strains of enterotoxigenicEscherichia coli(ETEC). To evaluate interventions for ETEC, experimental infection via a challenge model is critical. To our knowledge, there is a lack of explanation for the variability in responses observed across ETEC challenge studies. Our objective was to quantitatively summarize the responses and variability among recent ETEC challenge studies and develop a tool for sample size calculation. The most widely evaluated response criteria across ETEC challenge studies are growth performance, fecal consistency and bacterial shedding, intestinal morphology, and immune responses. Factors that contribute to …


Evaluating Hiphorius Phytase In Two Diet Formulation Strategies On Finishing Pig Growth Performance, Serum Chemistry, Bone Mineralization, And Carcass Characteristics, Macie E. Reeb, Jason C. Woodworth, Joel M. DeRouchey, Mike D. Tokach, Robert D. Goodband, Jordan T. Gebhardt, Jon R. Bergstrom 2023 Kansas State University, Manhattan

Evaluating Hiphorius Phytase In Two Diet Formulation Strategies On Finishing Pig Growth Performance, Serum Chemistry, Bone Mineralization, And Carcass Characteristics, Macie E. Reeb, Jason C. Woodworth, Joel M. Derouchey, Mike D. Tokach, Robert D. Goodband, Jordan T. Gebhardt, Jon R. Bergstrom

Kansas Agricultural Experiment Station Research Reports

Two experiments were conducted to determine the effects of HiPhorius (DSM Nutritional Products, Parsippany, NJ) phytase on finishing pig growth performance, serum chemistry, bone mineralization, and carcass characteristics. In Exp. 1, 1,161 pigs (PIC 337 × 1050; initially 80.9. ± 1.06 lb) were used in a 105-d trial. There were 27 pigs per pen and 10 or 11 replications per treatment. Treatments consisted of: 1) Control diet with no added phytase and formulated to NRC (2012) requirement estimates for standard total tract digestible (STTD) P; 2) 600 FYT/kg added phytase formulated to the same STTD P as the control diet …


Effects Of Increasing Soybean Meal Levels On Growth Performance And Carcass Characteristics Of Pigs In Grower And Late-Finishing Phases, Jamil E. G. Faccin, Mike D. Tokach, Joel M. DeRouchey, Jordan T. Gebhardt, Robert D. Goodband, Jason C. Woodworth 2023 Kansas State University, Manhattan

Effects Of Increasing Soybean Meal Levels On Growth Performance And Carcass Characteristics Of Pigs In Grower And Late-Finishing Phases, Jamil E. G. Faccin, Mike D. Tokach, Joel M. Derouchey, Jordan T. Gebhardt, Robert D. Goodband, Jason C. Woodworth

Kansas Agricultural Experiment Station Research Reports

Four experiments were conducted to determine the effects of increasing soybean meal (SBM) on grower and late-finishing pig performance. In Exp. 1, a total of 615 pigs (initially 95.2 ± 1.51 lb) were used in a 28-d trial with 14 replicate pens per treatment and 8 to 10 pigs per pen. Pens of pigs were randomly assigned to 1 of 5 dietary treatments which were corn-based with soybean meal levels of 19.1, 22.6, 26.3, 29.9, or 33.5%. In Exp. 2, a total of 615 pigs (initially 225.5 ± 3.42 lb) were used in a 30-d trial with 14 replicate pens …


Evaluating The Effects Of Soybean Meal Levels And Valine, Isoleucine, And Tryptophan Adjustment In Diets With Or Without Dried Distillers Grain Solubles On Finishing Pig Performance And Carcass Characteristics, Macie E. Reeb, Jason C. Woodworth, Joel M. DeRouchey, Mike D. Tokach, Robert D. Goodband, Jordan T. Gebhardt 2023 Kansas State University, Manhattan

Evaluating The Effects Of Soybean Meal Levels And Valine, Isoleucine, And Tryptophan Adjustment In Diets With Or Without Dried Distillers Grain Solubles On Finishing Pig Performance And Carcass Characteristics, Macie E. Reeb, Jason C. Woodworth, Joel M. Derouchey, Mike D. Tokach, Robert D. Goodband, Jordan T. Gebhardt

Kansas Agricultural Experiment Station Research Reports

A total of 1,080 pigs (PIC 337 × 1050; initially 58.4 ± 1.26 lb) were used in this 121-d experiment to determine the effects of added soybean meal (SBM) versus using an amino acid (AA) adjustment in diets with dried distillers grains with solubles (DDGS) on growth performance and carcass characteristics. Pens were randomly assigned to 1 of 4 dietary treatments in a completely randomized design. There were 27 pigs per pen and 10 replications per treatment. Treatments diets consisted of: 1) a control diet containing high SBM with no DDGS; 2) DDGS-based diet with a medium level of SBM; …


Understanding The Reduction Of Porcine Epidemic Diarrhea Virus, Porcine Reproductive And Respiratory Syndrome Virus, And Seneca Valley Virus 1 Rna In Inoculated Feed And The Environment Following Thermal Processing, Olivia L. Harrison, Haley K. Otott, Jianfa Bai, Vaughn A. Hamill, Aaron Singrey, Phillip C. Gauger, Marcelo N. Almeida, Jason C. Woodworth, Charles R. Stark, Roman M. Pogranichniy, Cassandra K. Jones, Jordan T. Gebhardt, Chad B. Paulk 2023 Kansas State University, Manhattan

Understanding The Reduction Of Porcine Epidemic Diarrhea Virus, Porcine Reproductive And Respiratory Syndrome Virus, And Seneca Valley Virus 1 Rna In Inoculated Feed And The Environment Following Thermal Processing, Olivia L. Harrison, Haley K. Otott, Jianfa Bai, Vaughn A. Hamill, Aaron Singrey, Phillip C. Gauger, Marcelo N. Almeida, Jason C. Woodworth, Charles R. Stark, Roman M. Pogranichniy, Cassandra K. Jones, Jordan T. Gebhardt, Chad B. Paulk

Kansas Agricultural Experiment Station Research Reports

Pelleting of feed has been demonstrated to be an effective mitigation strategy for porcine epidemic diarrhea virus (PEDV) contaminated feed but has not been evaluated for other endemic swine viruses like porcine reproductive and respiratory syndrome virus (PRRSV) or Seneca Valley virus 1 (SVV1). Therefore, the objective of this experiment was to evaluate the efficacy of pelleting to inactivate PEDV, PRRSV, and SVV1 inoculated feed. Ten replicates were conducted in the Cargill Feed Safety Research Center at Kansas State University (K-State) using a pilot scale mixer, bucket elevator, pellet mill (including conditioner and die), and cooler. First, a virus negative …


Effects Of Folic Acid On Nursery Pig Growth Performance And Serum Homocysteine, Larissa L. Becker, Jordan T. Gebhardt, Mike D. Tokach, Robert D. Goodband, Joel M. DeRouchey, Jason C. Woodworth 2023 Kansas State University, Manhattan

Effects Of Folic Acid On Nursery Pig Growth Performance And Serum Homocysteine, Larissa L. Becker, Jordan T. Gebhardt, Mike D. Tokach, Robert D. Goodband, Joel M. Derouchey, Jason C. Woodworth

Kansas Agricultural Experiment Station Research Reports

A total of 350 barrows (DNA 200 × 400; initially 13.2 ± 0.12 lb) were used in a 38-d growth study to determine the effects of folic acid on nursery pig growth performance and blood measurements. Pigs were weaned at approximately 21 d of age and randomly allotted to 1 of 5 dietary treatments in a completely randomized design. A total of 70 pens were used with 5 pigs per pen and 14 replications per treatment. Dietary treatments were corn-soybean meal-based and consisted of increasing folic acid: 0, 5, 10, 20, or 40 ppm. Treatment diets were fed in three …


Evaluating The Distribution Of Porcine Epidemic Diarrhea Virus, Porcine Reproductive And Respiratory Syndrome Virus, And Seneca Valley Virus 1 Inoculated Feed After The Use Of Physical Or Chemical Mitigants To Flush A Feed Manufacturing Facility, Olivia L. Harrison, Haley K. Otott, Jianfa Bai, Vaughn A. Hamill, Aaron Singrey, Phillip C. Gauger, Marcelo N. Almeida, Jason C. Woodworth, C. R. Stark, Roman M. Pogranichniy, Cassandra K. Jones, Jordan T. Gebhardt, Chad B. Paulk 2023 Kansas State University, Manhattan

Evaluating The Distribution Of Porcine Epidemic Diarrhea Virus, Porcine Reproductive And Respiratory Syndrome Virus, And Seneca Valley Virus 1 Inoculated Feed After The Use Of Physical Or Chemical Mitigants To Flush A Feed Manufacturing Facility, Olivia L. Harrison, Haley K. Otott, Jianfa Bai, Vaughn A. Hamill, Aaron Singrey, Phillip C. Gauger, Marcelo N. Almeida, Jason C. Woodworth, C. R. Stark, Roman M. Pogranichniy, Cassandra K. Jones, Jordan T. Gebhardt, Chad B. Paulk

Kansas Agricultural Experiment Station Research Reports

Contaminated feed is a route of virus transmission between feed mills and swine farms. To reduce the risk of transmission, an understanding of the virus distribution and mitigation strategies are needed. The objective of this study was to evaluate the distribution of porcine epidemic diarrhea virus (PEDV), porcine reproductive and respiratory syndrome virus (PRRSV), and Seneca Valley virus 1 (SVV1) inoculated feed in the environment and feed of a feed mill before and after the use of chemical mitigants. A 50-lb batch of feed was run through a mixer and bucket elevator followed by a batch inoculated with PEDV, PRRSV, …


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