Medicine and Health Sciences Commons^™

Qol In Child Lqts Patients Compared To Cardiac Patients, Elizabeth A. Waldron, Stephanie H. Felgoise, Carmella Tress, Katherine Lawrence, Victoria L. Vetter

Research Day

Introduction:

• Long QT Syndrome (LQTS) is a life threatening geneticallyinherited cardiac arrhythmia disorder affecting approximately 1:2500 persons1, often diagnosed in childhood.

• Management of LQTS changes patients’ lifestyles which can affect quality of life (QoL). Patients have restrictions in physical activity, diet, treatment of noncardiac conditions; take daily doses of medicine and/or have implantable cardiac devices (pacemaker/defibrillator).

• General pediatirc cardiac patients show significantly worse QoL in comparison to healthy controls2,3.

• Nearly 1 in 5 with other cardiac disorders reported impaired psychosocial functioning3.

• The effects of implanted cardiac rhythm devices in cardiac patients demonstrated a significant effect …

Changes In Expression Of Genes Associated With Autophagy And Apoptosis In Neuronal Cells Infected With Hsv-1may Suggest Infection-Induced Mechanisms Of Neurodegeneration, Alexis Mark, Fiora D. Zoga, Brian J. Balin Phd, Denah M. Appelt Phd, Susan T. Hingley

Research Day

Background:This study investigates the potential role of herpes simplex virus type 1 (HSV-1) in the pathogenesis of neurodegenerative disorders, such as Alzheimer’s disease (AD), by exploring changes in gene expression related to antiviral immunity and the autophagic pathway. Autophagy is a process that recycles organelles and proteins to create more energy for the cell. This pathway has been linked to neurodegeneration, as malfunctions in the completion of this process lead to a decline in overall cellular health and function. Interestingly, HSV-1 has been shown to block the completion of autophagy, which would potentially contribute to the cytopathic changes observed …

The Effects Of Dihydrobiopterin And Tetrahydrobiopterin On Hydrogen Peroxide And Nitric Oxide Release During Extracorporeal Shockwave Lithotripsy, Brittany L. Deiling, Edward S. Iames, Kerry-Anne Perkins, Qian Chen Phd, Lindon H. Young Phd

Research Day

Extracorporeal shockwave lithrotripsy (ESWL) is an effective, non-invasive clinical therapy utilized to break up stones in the kidney and urinary tract. A lithotripter generates high-energy acoustic pulses and propagates those shock waves through a lens on a region that focuses on the location of the stone, in turn breaking up the stone. The successive pulses generate shearing forces and cavitation bubbles. Cavitation bubbles are the formation and implosion of liquid free zones. The cavitation bubbles implode rapidly to create their own shockwaves that also put pressure on the stone. After treatment, fragmentation of the stone allows the debris to be …

Mechanisms Of Mouse Hepatitis Virus Entry Into Cells, Philip J. Koehler, Kristina Cade, Fiora D. Zoga, Susan T. Hingley

Research Day

Background: Viruses can enter cells through several mechanisms, two common ones being clathrin-mediated and caveolin-mediated endocytosis. The clathrin pathway delivers viral particles to endosomes, with subsequent acidification of the endosome and endosome/lysosome fusion often a prerequisite for release of the viral genome into the cytoplasm. The caveolin-mediated pathway delivers virus initially into vesicles called caveosomes, which have a neutral pH, before viral uncoating occurs. Viral entry pathways can be examined by using various drugs to inhibit the different endocytosis pathways, as well as by siRNA technology to down-regulate expression of clathrin or caveolin proteins on the surface of host …

Characterization Of A Small Iron Protein, Pyrococcus Furiosus Rubredoxin, As A Potential Cancer Drug Delivery System, Siri L. Chirumamilla, David L. Blum, Ray L. Mernaugh, Francis E. Jenney

Research Day

Background: Cancer is an elusive neoplastic disease that claims the lives of many people around the world every year. Though treatments have become more specific to the different types of cancer, the need for antineoplastic drugs that target cancer cells and leave normal cells unharmed, with little to no systemic toxicity remains, and rubredoxin might be such a tool. Rubredoxin is a small (53 amino acids), water soluble, non-heme iron electron transfer protein that contains an iron atom cofactor, which can be substituted with various cytotoxic transition metals such as nickel and cobalt with little or no effect on …

Predicting Acute Urinary Retention In Patients With Elevated Post Void Residuals, David B. Cahn Mbs, Curtis P. Ross Do, Michelle L. Persun Md, Philip C. Ginsberg Jd, Do

Research Day

Objectives: To perform a retrospective analysis in order to evaluate factors that may help predict which men with elevated PVRs that were at increased risk to develop AUR.

Imp2 Expression In The Mouse Nervous System, Sarah E. Chmielewski, Paige E. Black, Tiffany M. Holmes, Kelly S. Lau, Gary J. Bassell

Research Day

Background: Insulin-like growth factor-II (IGF-II) mRNA-binding protein-2 (IMP2) is one of the three homologs (IMP1-3) that play important roles in the posttranscriptional regulation of gene expression in several tissues. IMP1/ZBP1 (zipcode binding protein) has been shown to play important roles in axon guidance and regeneration by regulating the localization and translation of specific mRNAs. However, the function of IMP2 is least understood, largely because an isoform-specific antibody is not available, which makes the conventional techniques to locate protein expression not feasible.

An Analysis Of Functional Status In Multiple Sclerosis Patients After Progressive Non-Aerobic High-Intensity Maximal Effort Exercise (Mee), Hilda Lai, Michael L. Kuchera, R T. Dombroski, T Vardy, F Thomas, S O’Brien, P Yagnik, K Wenzel, S Stoll, Bruce C. Stouch, M. T. Wallin, I. Vargas,, Olufolake Odutola

Research Day

Background: Multiple Sclerosis (MS) is a disease with a wide-ranging impact on functional status. MS patient function has been assessed using Multiple Sclerosis Functional Composite Score (MSFCS). The MSFCS includes the standardized scores (Z-score) of three functional tests: the Paced Auditory Serial Addition Test (PASAT-3”) for cognitive function, 9-Hole Peg Test (9-HPT) for upper extremity function, and timed 25-foot walk (25-TW) for lower extremity function. One of the most common symptoms experienced by MS patients is severe fatigue, often brought on suddenly by aerobic exercise. Non-aerobic maximal effort exercise (MEE) is thought to increase strength without increasing fatigue. The …

The Effect Of Progressive Non-Aerobic High-Intensity Maximal Effort Exercise (Mee) On The Health-Related Quality Of Life In Patients With Multiple Sclerosis, Olufolake Odutola, Michael L. Kuchera, R T. Dombroski, T Vardy, F Thomas, S O’Brien, P Yagnik, K Wenzel, S Stoll, Bruce C. Stouch, M T. W, I Vargas, Hilda Lai

Research Day

Background: Studies indicate that Multiple Sclerosis (MS) patients are less satisfied with the quality of their lives than healthy individuals in similar circumstances. Common symptoms experienced include fatigue, cognitive dysfunction, pain, spasticity, depression, bladder/bowel dysfunction and sexual dysfunction. Several pharmacological and non-pharmacological methods have been employed for such symptoms to try to increase quality of life and reduce the mortality rate. Non-pharmacological methods recommended for MS patients include lifestyle modifications, exercise programs and physical therapy. MS patients easily fatigue during aerobic exercise but a non-aerobic progressive maximal effort exercise (MEE) protocol consisting of a few short, duration isometric and eccentric …

Infection With Chlamydia Pneumoniae Alters Calcium-Associated Gene Regulation And Processes In Neuronal Cells And Monocytes: Implications For Alzheimer’S Disease, Keith G. Williams, Juliana Zoga, Susan T. Hingley, Brian J. Balin, Denah M. Appelt, Marcus G. Bell

Research Day

Background: First proposed by Khachaturian in 1994, the calcium hypothesis postulates that sustained disturbance of intracellular calcium is the leading cause of neurodegenerative disorders. Studies showing alteration in calcium signaling in both sporadic and familial Alzheimer’s disease (AD) support this hypothesis. Intracellular calcium signaling is tightly regulated in time, intensity, and space, and is responsible for a variety of neuronal functions. Calcium influx from the extracellular environment modulates calcium levels, as do intracellular stores in the endoplasmic reticulum. The focus of this study was to test various calcium related genes in both monocytes and neuronal cells. Previous studies have shown …

Documenting Pressures Used For Manual Diagnosis And Treatment Of Cervical Spine Somatic Dysfunction, Joseph Yoha, Michael L. Kuchera, Precious Barnes, Frank Casella, Jeffrey A. Nelson

Research Day

Background: Palpatory assessment of free or restricted motion patterns is part of the diagnosis of spinal somatic dysfunction (SD). Diagnostically, local soft tissues are compressed (pre-loaded) over the structure of interest followed by one or more test impulses to assess the quality of the “end-feel” motion in several planes. These barrier sensations are often described qualitatively but have not been objectively quantified. Noninvasive, tactile pressure sensors built into a digital palpation monitoring system (IsoTOUCH®; Neuromuscular Engineering; Nashville TN, USA) were used to document loading and impulse pressures for palpatory segmental diagnosis and to first engage and then quickly move …

Effects Of Non-Aerobic Maximal Effort Exercise On Fatigue In Deconditioned Men And Women With Multiple Sclerosis, Jessica U. Korsh, Michael L. Kuchera, R T. Dombroski, T Vardy, F Thomas, S O’Brien, P Yagnik, K Wenzel, S Stoll, Bruce C. Stouch, M T. Wallin, Jeffrey A. Nelson

Research Day

Multiple Sclerosis (MS) is a neurodegenerative disease of unknown etiology affecting women more frequently than men. Mental and physical fatigue complaints are often the most disabling symptoms for an MS patient. Both are multifactorial, potentially exacerbated by aerobic exercise, may prevent sustained physical functioning, and significantly interfere with activities of daily living1. A multi-center study was designed to investigate the effects of non-aerobic maximal effort exercise (MEE) for deconditioned persons with MS, with the expectation of minimizing fatigue. The IsoPUMP (Neuromuscular Engineering; Nashville, TN), is a specialized exercise and strength-sensing machine, designed to allow individuals to safely perform and record …

The Human Pathogen Clostridium Dificle: A Look At A Putative Involved In Protection From Oxidative Stress, Tashinea Lee, Francis E. Jenney

Research Day

Background: Hospital acquired infections due to Clostridium difficile (C. diff) is associated with nosocomial diarrhea and pseudomembranous colitis. Using a published microarray analysis of C. diff strain 630, several open reading frames (ORFs) were noted for their upregulation under different environmental stresses. One of these genes (CD1134) is a putative glyoxalase I. The glyoxalase enzymes detoxify a side product of glycolysis, methylglyoxal, and use metals as a cofactor.

Effect Of Prior Anterior Superior Iliac Spine Compression Testing On Second Assessor Findings: Implications For Inter-Examiner Reliability Testing, Frank Casella Ms, Michael L. Kuchera Do, Faao, Jeffrey A. Nelson, Veronica Ferencz Mba, Nicole E. Myers

Research Day

BACKGROUND: Osteopathic physicians use palpation to diagnose sacroiliac joint somatic dysfunction (SD) -- including the Anterior Superior Iliac Spine (ASIS) Compression Test for dysfunctional side lateralization. (Literature suggests right-sided lateralization in 80% of asymptomatic individuals). Accurate, reliable tests are crucial however to diagnose SD and kappa (κ) analysis is a gold-standard to determine the degree of interexaminer reliability for tests. Few studies have examined the effect the palpatory examination has on subsequent diagnostic findings and therefore on κ-values.

Inter-Examiner Reliability Of An Anterior Superior Iliac Spine Compression Test Used To Lateralize Pelvic Somatic Dysfunction To The Right Side Or Not, Frank Casella Ms, Michael L. Kuchera Do, Faao, Nicole E. Myers, Jeffrey A. Nelson, Veronica Ferencz Mba

Research Day

BACKGROUND: Osteopathic physicians use a number of palpatory structural examinations to diagnose pelvic somatic dysfunction (SD). They may elect to use the Anterior Superior Iliac Spine (ASIS) Compression Test to lateralize the dysfunctional side. Accurate, reliable tests are crucial to neuromusculoskeletal diagnosis and this study employs the kappa (κ) analysis protocol recommended for assessing interexaminer reliability of manual medicine tests (published by the Fédération Internationale de Médecine Manuelle [FIMM]). κ-values ≥0.40 (moderate agreement) are considered to be acceptable for use in the clinical setting.

The Use Of Objective Data To Improve Interexaminer Reliability, Veronica Ferencz Mba, Frank Casella Ms, Jeffrey A. Nelson, Nicole E. Myers, Michael L. Kuchera Do, Faao

Research Day

BACKGROUND: In Osteopathic Manipulative Medicine (OMM) and Manual/Musculoskeletal Medicine (MMM), palpatory diagnosis is performed on a regular basis to diagnose somatic dysfunction (SD). This examination requires careful and precise touch coupled with subjective interpretation by individual examiners who may have been trained to evaluate SD through different methods. Interexaminer reliability studies aim to minimize variance by providing quantifiable scientific data to evaluate specific test protocols which can then be taught to practitioners. In a previous PCOM study, two examiners independently diagnosed innominate bone dysfunction lateralized using the ASIS compression test on a large group of subjects. A pressure monitoring system …

Comparing Inter-Examiner Reliability Levels When Diagnosing Male & Female Innominate Dysfunctions Using A Hemi-Pelvise Compression Lateralization Test And Pelvic Landmark Levels., Nicole E. Myers, Michael L. Kuchera Do, Faao, Veronica Ferencz Mba, Jeffrey A. Nelson, Frank Casella Ms

Research Day

BACKGROUND: When diagnosing innominate somatic dysfunctions it may be relevant to recognize that structural, functional, and hormonal differences exist between male and female pelvises. The female pelvis is less massive, ilia are less sloped, and female hormones influence ligamentous tension. Despite these differences, few studies have analyzed gender effects on inter-examiner reliability when using palpatory diagnosis to diagnose innominate dysfunctions. In this study, we hypothesized that interexaminer reliability would be higher in male subjects than in female subjects due cyclic variability of hormonal influence of ligamentous tension in the female pelvis. The kappa (κ) statistic was selected to evaluate …