Optics Commons^™

Resolving The Nanoscale Structure Of Β-Sheet Peptide Self-Assemblies Using Single-Molecule Orientation–Localization Microscopy, Weiyan Zhou, Conor L. O'Neill, Tianben Ding, Oumeng Zhang, Jai S. Rudra, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Synthetic peptides that self-assemble into cross-β fibrils are versatile building blocks for engineered biomaterials due to their modularity and biocompatibility, but their structural and morphological similarities to amyloid species have been a long-standing concern for their translation. Further, their polymorphs are difficult to characterize using spectroscopic and imaging techniques that rely on ensemble averaging to achieve high resolution. Here, we utilize Nile red (NR), an amyloidophilic fluorogenic probe, and single-molecule orientation-localization microscopy (SMOLM) to characterize fibrils formed by the designed amphipathic enantiomers KFE8^L and KFE8^D and the pathological amyloid-beta peptide Aβ42. Importantly, NR SMOLM reveals the helical (bilayer) …

6d Single-Fluorogen Orientation-Localization Microscopy For Elucidating The Architecture Of Beta-Sheet Assemblies And Biomolecular Condensates, Tingting Wu, Weiyan Zhou, Jai S. Rudra, Rohit V. Pappu, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

We develop six-dimensional single-molecule orientation-localization microscopy (SMOLM) to measure the 3D positions and 3D orientations simultaneously of single fluorophores. We show how careful optimization of phase and polarization modulation components can encode phase, polarization, and angular spectrum information from each fluorescence photon into a microscope’s dipole-spread function. We used the transient binding and blinking of Nile red (NR) to characterize the helical structure of fibrils formed by designed amphipathic peptides, KFE8^L and KFE8^D, and the pathological amyloid-beta peptide Aβ42. We also deployed merocyanine 540 to uncover the interfacial architectures of biomolecular condensates.

Laboratory Exercise For The Radiometry Student, Michael A. Marciniak

Faculty Publications

The U.S. Air and Space Forces require optical expertise among their personnel. The Air Force Institute of Technology offers a graduate optics curriculum, which includes a three-course sequence to educate students in the optical concepts of radiometry and radiometric instrumentation. We find radiometry is often a deceptively difficult concept for students to master. To address this, we have developed an experiment in our optics-laboratory coursework to help them gain this mastery. A Fourier-transform infrared spectrometer (FTS) is used to collect spectral data from an unknown sample. FTS calibration and data collection are discussed here, as are the two specific samples …

Modelling Hoe Performance With An Extended Source; Experimental Investigation Using Misaligned Point Sources, Jorge Lasarte, Kevin Murphy, Izabela Naydenova, Maria Victoria Collados, Jesús Atencia, Suzanne Martin

Articles

Holographic optical elements (HOEs) have the potential to enable more compact, versatile, and lightweight optical designs, but many challenges remain. Volume HOEs have the advantage of high diffraction efficiency, but they present both chromatic selectivity and chromatic dispersion, which impact their use with wide spectrum light sources. Single-color light emitting diode (LED) sources have a narrow spectrum that reduces these issues and this makes them better suited for use with volume HOEs. However, the LED source size must be taken into consideration for compact volume HOE-LED systems. To investigate the design limits for compact HOE-LED systems, a theoretical and experimental …

Two-Photon Absorption And Fluorescence Of Cadmium Sulfide, Jacob Goranson, Gregory J. Taft

Physics Faculty Publications

The two-photon absorption and fluorescence of bulk cadmium sulfide were studied using 50-fs, 800-nm pulses from an unamplified Ti:sapphire laser. The fluorescence spectrum was measured to have a main peak at 522 nm, and the power of the fluorescence was shown to vary quadratically with the 800-nm beam power. This supports the theory that the fluorescence is excited by two-photon absorption and confirms previous work done with longer duration, higher energy excitation pulses. Pump-probe measurements provided additional confirmation of the two-photon absorption. Measured spectral broadening of the wings of the laser spectrum also was observed, which likely is due to …

Exciton Dynamics, Interaction, And Transport In Monolayers Of Transition Metal Dichalcogenides, Saroj Chand

Dissertations, Theses, and Capstone Projects

Monolayers Transition metal dichalcogenides (TMDs) have attracted much attention in recent years due to their promising optical and electronic properties for applications in optoelectronic devices. The rich multivalley band structure and sizable spin-orbit coupling in monolayer TMDs result in several optically bright and dark excitonic states with different spin and valley configurations. In the proposed works, we have developed experimental techniques and theoretical models to study the dynamics, interactions, and transport of both dark and bright excitons.

In W-based monolayers of TMDs, the momentum dark exciton cannot typically recombine optically, but they represent the lowest excitonic state of the system …

Residual Optical Absorption From Native Defects In Cdsip2 Crystals, Timothy D. Gustafson, Nancy C. Giles, Elizabeth M. Scherrer, Kevin T. Zawilski, Peter G. Schunemann, Kent L. Averett, Jonathan E. Slagle, Larry E. Halliburton

Faculty Publications

CdSiP₂ crystals are used in optical parametric oscillators to produce tunable output in the mid-infrared. As expected, the performance of the OPOs is adversely affected by residual optical absorption from native defects that are unintentionally present in the crystals. Electron paramagnetic resonance (EPR) identifies these native defects. Singly ionized silicon vacancies (V^-_Si) are responsible for broad optical absorption bands peaking near 800, 1033, and 1907 nm. A fourth absorption band, peaking near 630 nm, does not involve silicon vacancies. Exposure to 1064 nm light when the temperature of the CdSiP₂ crystal is near 80K converts …

Development Of Advanced Instrumentation For High-Enthalpy Flow Characterization For The Onr-Uta Arc-Jet ”Leste” Facility, Ian Raybon

Mechanical and Aerospace Engineering Dissertations - Archive

Sustained hypersonic flight is an exceedingly challenging task comprised of a myriad of complex physical processes. At hypersonic speeds, which is defined by the presence of unique phenomena like high-temperature nonequilibrium flow, viscous and vorticity interactions, and thin shock layers, which are not seen in supersonic, transonic, or subsonic vehicles, the vehicle design is principally driven by the high levels of aerodynamic heating caused by the conversion of kinetic energy to internal energy. Gaining an understanding of how these physical processes interact with each other is required to develop a design methodology for this class of vehicles. Arc-jet wind tunnels …

Optical Seed Development For Yb-Fiber Laser, James G. Brutus

Graduate Thesis and Dissertation 2023-2024

Master Oscillator Power Amplifiers (MOPA) are laser systems that utilize a seed and pump amplification system to boost the output power of high-quality lower power seeding signals. MOPAs can generate high gain while avoiding many of the nonlinearities that negatively affect resonance-based lasers that are known to feature higher internal intensities. Additionally, MOPAs provide an easy alternative to the construction of novel laser technologies for higher output power as they can be easily combined with existing laser sources to amplify their output power.

This thesis outlines the design of an ytterbium-doped fiber laser (YDFL), featuring a MOPA architecture. The YDFL …

Visual Experience Enhancement In Augmented Reality Displays, Qian Yang

Graduate Thesis and Dissertation 2023-2024

In the dynamic arena of display technology, augmented reality (AR) displays represent a pivotal advancement, seamlessly bridging the digital and physical worlds. This dissertation delves into the realm of AR display technologies, spotlighting the challenges and limitations of current systems, including transparent and near-eye displays, and proposes innovative solutions to enhance user experience and display performance. With a focus on overcoming issues such as diffraction-induced image blur, the trade-off between resolution and field of view (FoV) in near-eye displays, and FoV constraints in waveguide-based displays, this research introduces new evaluation methods, optimization techniques, and system designs. First, the dissertation presents …

Dual Frequency Comb Mid-Ir – Thz Spectroscopy, Dmitrii Konnov

Graduate Thesis and Dissertation 2023-2024

The optical frequency comb is a coherent light source whose spectrum consists of hundreds of thousands perfectly equidistant narrow frequency components and precisely expressed in just two radio frequencies. Even though optical frequency combs were developed 25 years ago, that led to the Nobel Prize in Physics 2005, only recently there was a significant progress in generating broadband optical frequency combs in the mid-infrared. These achievements became possible due to the development of new types of robust fiber and solid-state lasers and the efficient downconverting of their frequencies through different techniques based on advanced nonlinear crystals. In this dissertation, I …

Human Visual Search Performance For Close Range Detection Of Static Targets From Moving Sensor Platforms, Jennifer Hewitt

Graduate Thesis and Dissertation 2023-2024

Search models based on human perception have been developed by military researchers over the past few decades and have both military and commercial applications for sensor design and implementation. These models are created primarily for static imagery, and accurately predict task performance for systems with stationary targets and stationary sensors, if the observer is given infinite time to make targeting decisions. To account for situations where decisions must be made on a shortened time scale, the time-limited search model was developed to describe how task performance evolves with time. Recent variations of this model have been made to account for …

The Weighty Words Of Lord Rayleigh, John Adam

Mathematics & Statistics Faculty Publications

[First paragraph] Lord Rayleigh, a.k.a. John William Strutt, 3rd Baron Rayleigh (1842–1919), was, in my opinion, a quintessential mathematical and experimental physicist and, indeed, a classical applied mathematician of the highest order for his era. Among the many honors he received, the most prestigious was the 1904 Nobel Prize in Physics “for his investigations of the densities of the most important gases and for his discovery of argon in connection with these studies.” He was one of the very few members of so-called British “higher nobility” who won fame as an outstanding scientist.

Sky Smiley Face: Solutions For Fermi Questions, November 2024, John Adam

Mathematics & Statistics Faculty Publications

Question 2(a): By rotating Fig. 2 counterclockwise by 90°, explain why circumhorizontal arcs (CHAs) [Figs. 1(b) and (c)] form (under favorable conditions) only when the solar altitude exceeds 58° of arc. (In this case, the incoming ray enters a vertical face of an ice crystal and exits via the lower horizontal face.)Question 2(b): By considering the tilt of Earth’s imaginary N–S axis toward the plane of its orbit around the Sun (∼23.5°), explain why in the northern hemisphere CHAs cannot be seen above about latitude 56° N.

Next-Generation High-Performance Virtual Reality And Augmented Reality Light Engines, Zhiyong Yang

Graduate Thesis and Dissertation 2023-2024

The immersive virtual reality (VR) and the optical see-through augmented reality (AR) are expected to revolutionize human lives in work, education, entertainment, healthcare, spatial computing, and digital twins, just to name a few. Next-generation VR/AR devices should exhibit a wide field-of-view (FoV), crisp image without screen-door effect, high dynamic range, compact form factor and lightweight, and low power consumption. Such demanding requirements pose a significant challenge to traditional direct-view display panels. To address these technical challenges, novel approaches need to be proposed. This dissertation is devoted to developing next-generation high-performance display light engines toward high resolution density, high optical efficiency, …

Intelligent Millimeter-Wave System For Human Activity Monitoring For Telemedicine, Abdullah K. Alhazmi, Mubarak A. Alanazi, Awwad H. Alshehry, Saleh M. Alshahry, Jennifer Jaszek, Cameron Djukic, Anna Brown, Kurt Jackson, Vamsy P. Chodavarapu

Electrical and Computer Engineering Faculty Publications

Telemedicine has the potential to improve access and delivery of healthcare to diverse and aging populations. Recent advances in technology allow for remote monitoring of physiological measures such as heart rate, oxygen saturation, blood glucose, and blood pressure. However, the ability to accurately detect falls and monitor physical activity remotely without invading privacy or remembering to wear a costly device remains an ongoing concern. Our proposed system utilizes a millimeter-wave (mmwave) radar sensor (IWR6843ISK-ODS) connected to an NVIDIA Jetson Nano board for continuous monitoring of human activity. We developed a PointNet neural network for real-time human activity monitoring that can …

Exponential Fusion Of Interpolated Frames Network (Efif-Net): Advancing Multi-Frame Image Super-Resolution With Convolutional Neural Networks, Hamed Elwarfalli, Dylan Flaute, Russell C. Hardie

Electrical and Computer Engineering Faculty Publications

Convolutional neural networks (CNNs) have become instrumental in advancing multi-frame image super-resolution (SR), a technique that merges multiple low-resolution images of the same scene into a high-resolution image. In this paper, a novel deep learning multi-frame SR algorithm is introduced. The proposed CNN model, named Exponential Fusion of Interpolated Frames Network (EFIF-Net), seamlessly integrates fusion and restoration within an end-to-end network. Key features of the new EFIF-Net include a custom exponentially weighted fusion (EWF) layer for image fusion and a modification of the Residual Channel Attention Network for restoration to deblur the fused image. Input frames are registered with subpixel …

Measurement Of Ascorbic Acid Samples' Optical Rotation Via An Improvised Polarimeter For Purity Assessment, Ryan Felicidario, Ramon M. Delos Santos

Physics Faculty Publications

The polarization of light finds practical application in physics and chemistry through the optical activity phenomenon, where polarimeters play a crucial role. This research builds on the improvised polarimeter designed by Kvittingen and Sjursnes, implemented with relevant modifications, to measure optical rotations of over-the-counter ascorbic acid samples. The study aims to assess the purity of two brands of ascorbic acid through polarimetry, comparing the calculated specific rotation with the literature values and supplementing the characterization with melting point determination. The constructed polarimeter, assembled using Lego bricks, provides an affordable alternative for educational purposes, addressing the challenges observed in the accessibility …

Analysis Of Macular Pigment Carotenoids In Human Blood Serum Of Glaucoma Patients As A Measure Of Ocular Health: A Raman Spectroscopic Study., Joy Udensi, Ekaterina Loskutova, James Loughman, Hugh J. Byrne

Datasets

Carotenoids are a major component of the human diet and have been widely studied for their antioxidant, and vision protection roles in the human body, and dietary supplementation is promoted in particular for ocular health. An initial trial (European Nutrition in Glaucoma Management (ENIGMA)), which assessed macular pigment optical density (MPOD) as well as ocular structural, functional and perceptual parameters before and after the 18-month supplementation of glaucoma patients with macular pigment (MP) carotenoids (lutein, zeaxanthin, meso-zeaxanthin), confirmed supplementation significantly improved the clinical ocular health of participants. Blood contains all major dietary carotenoids, presenting it as a suitable and efficient …

Applications Of Independent And Identically Distributed (Iid) Random Processes In Polarimetry And Climatology, Dan Kestner

Dissertations, Master's Theses and Master's Reports

The unifying theme of this thesis is the characterization of “perfect randomness,” i.e., independent and identically distributed (IID) stochastic processes as these are applied in physical science. Two specific and mathematically distinct applications are chosen: (i) Radar and optical polarimetry; (ii) Analysis of time series in meteorology. In (i), IID process of a special kind, namely, with a distribution defined by symmetry, is used to link its multivariate Gaussian density to uniformity on the Poincaré sphere. This “statistical ellipsometry” approach is then used to relate polarimetric mismatches or imbalances to ellipsometric variables and suitably chosen cross-correlation measures. In (ii), recently …